Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036389.01.T01 | XP_024626106.1 | 82.752 | 487 | 42 | 2 | 1 | 466 | 1 | 466 | 0 | 809 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036389.01.T01 | A0A396GV01 | 79.466 | 487 | 41 | 2 | 1 | 466 | 1 | 449 | 0.0 | 770 |
| MsG0780036389.01.T01 | G7L479 | 80.493 | 487 | 39 | 3 | 1 | 466 | 1 | 452 | 0.0 | 770 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036389.01.T01 | MTR_7g012680 | 80.493 | 487 | 39 | 3 | 1 | 466 | 1 | 452 | 0.0 | 770 |
| MsG0780036389.01.T01 | MTR_7g066570 | 73.663 | 486 | 105 | 3 | 1 | 464 | 1 | 485 | 0.0 | 701 |
| MsG0780036389.01.T01 | MTR_7g066260 | 56.540 | 474 | 180 | 8 | 6 | 465 | 7 | 468 | 3.87e-170 | 488 |
| MsG0780036389.01.T01 | MTR_7g058710 | 54.108 | 499 | 173 | 9 | 6 | 466 | 4 | 484 | 9.12e-169 | 484 |
| MsG0780036389.01.T01 | MTR_7g066340 | 57.839 | 472 | 173 | 7 | 5 | 465 | 3 | 459 | 1.63e-163 | 470 |
| MsG0780036389.01.T01 | MTR_7g066350 | 58.297 | 458 | 162 | 10 | 10 | 455 | 5 | 445 | 1.16e-159 | 460 |
| MsG0780036389.01.T01 | MTR_1g041315 | 53.219 | 466 | 180 | 9 | 1 | 443 | 1 | 451 | 5.53e-148 | 431 |
| MsG0780036389.01.T01 | MTR_7g066580 | 50.936 | 481 | 167 | 9 | 20 | 465 | 1 | 447 | 1.89e-147 | 428 |
| MsG0780036389.01.T01 | MTR_7g066290 | 52.878 | 469 | 172 | 6 | 2 | 459 | 3 | 433 | 3.69e-145 | 422 |
| MsG0780036389.01.T01 | MTR_1g014060 | 50.337 | 445 | 158 | 6 | 23 | 466 | 9 | 391 | 1.48e-137 | 401 |
| MsG0780036389.01.T01 | MTR_7g066400 | 51.404 | 463 | 171 | 11 | 10 | 455 | 8 | 433 | 1.45e-135 | 398 |
| MsG0780036389.01.T01 | MTR_7g058700 | 49.251 | 467 | 142 | 8 | 26 | 466 | 531 | 928 | 3.39e-128 | 394 |
| MsG0780036389.01.T01 | MTR_7g066360 | 50.317 | 473 | 197 | 10 | 1 | 459 | 1 | 449 | 6.31e-128 | 380 |
| MsG0780036389.01.T01 | MTR_7g066310 | 49.127 | 458 | 158 | 10 | 11 | 465 | 4 | 389 | 3.18e-126 | 373 |
| MsG0780036389.01.T01 | MTR_7g066430 | 44.785 | 489 | 231 | 10 | 14 | 465 | 4 | 490 | 3.84e-123 | 368 |
| MsG0780036389.01.T01 | MTR_7g066430 | 28.512 | 242 | 143 | 8 | 4 | 224 | 247 | 479 | 1.62e-14 | 75.9 |
| MsG0780036389.01.T01 | MTR_2g029400 | 45.549 | 483 | 151 | 9 | 1 | 449 | 1 | 405 | 4.08e-116 | 347 |
| MsG0780036389.01.T01 | MTR_7g066410 | 43.738 | 503 | 224 | 11 | 15 | 459 | 11 | 512 | 9.31e-113 | 342 |
| MsG0780036389.01.T01 | MTR_7g066410 | 29.339 | 242 | 139 | 8 | 4 | 224 | 276 | 506 | 8.69e-16 | 80.1 |
| MsG0780036389.01.T01 | MTR_7g062300 | 37.838 | 481 | 253 | 10 | 6 | 455 | 4 | 469 | 9.24e-96 | 297 |
| MsG0780036389.01.T01 | MTR_7g062280 | 38.294 | 504 | 253 | 12 | 6 | 466 | 4 | 492 | 2.69e-95 | 296 |
| MsG0780036389.01.T01 | MTR_7g063660 | 39.220 | 487 | 247 | 11 | 13 | 466 | 8 | 478 | 8.07e-94 | 292 |
| MsG0780036389.01.T01 | MTR_7g063660 | 26.818 | 220 | 140 | 6 | 251 | 451 | 4 | 221 | 3.32e-15 | 78.2 |
| MsG0780036389.01.T01 | MTR_7g063900 | 40.733 | 491 | 242 | 13 | 14 | 466 | 10 | 489 | 2.06e-92 | 289 |
| MsG0780036389.01.T01 | MTR_7g063900 | 27.523 | 218 | 135 | 8 | 255 | 451 | 9 | 224 | 9.93e-13 | 70.5 |
| MsG0780036389.01.T01 | MTR_7g063840 | 38.134 | 493 | 253 | 16 | 5 | 466 | 3 | 474 | 2.68e-90 | 283 |
| MsG0780036389.01.T01 | MTR_7g066480 | 41.126 | 462 | 206 | 12 | 13 | 461 | 15 | 423 | 3.39e-90 | 281 |
| MsG0780036389.01.T01 | MTR_7g063920 | 38.259 | 494 | 220 | 12 | 1 | 466 | 1 | 437 | 6.07e-88 | 276 |
| MsG0780036389.01.T01 | MTR_7g063890 | 40.472 | 509 | 243 | 15 | 2 | 465 | 11 | 504 | 7.29e-87 | 275 |
| MsG0780036389.01.T01 | MTR_7g063880 | 39.679 | 499 | 232 | 20 | 1 | 463 | 1 | 466 | 3.56e-86 | 272 |
| MsG0780036389.01.T01 | MTR_7g063820 | 40.361 | 498 | 243 | 17 | 8 | 466 | 3 | 485 | 4.16e-85 | 270 |
| MsG0780036389.01.T01 | MTR_7g063830 | 37.473 | 467 | 245 | 13 | 8 | 459 | 4 | 438 | 7.14e-84 | 265 |
| MsG0780036389.01.T01 | MTR_3g007740 | 39.355 | 465 | 234 | 14 | 13 | 459 | 8 | 442 | 4.17e-83 | 263 |
| MsG0780036389.01.T01 | MTR_7g066280 | 40.749 | 427 | 168 | 11 | 93 | 465 | 1 | 396 | 1.02e-82 | 261 |
| MsG0780036389.01.T01 | MTR_7g066490 | 40.708 | 452 | 206 | 13 | 14 | 461 | 16 | 409 | 1.13e-82 | 261 |
| MsG0780036389.01.T01 | MTR_4g019310 | 37.103 | 504 | 236 | 20 | 11 | 455 | 5 | 486 | 2.11e-76 | 248 |
| MsG0780036389.01.T01 | MTR_4g019310 | 28.968 | 252 | 128 | 10 | 10 | 230 | 262 | 493 | 6.27e-14 | 74.3 |
| MsG0780036389.01.T01 | MTR_3g065200 | 36.184 | 456 | 222 | 11 | 12 | 446 | 1 | 408 | 2.78e-75 | 243 |
| MsG0780036389.01.T01 | MTR_7g063670 | 38.445 | 476 | 244 | 17 | 10 | 466 | 13 | 458 | 1.20e-74 | 242 |
| MsG0780036389.01.T01 | MTR_7g066680 | 49.158 | 297 | 102 | 3 | 208 | 455 | 38 | 334 | 1.64e-74 | 238 |
| MsG0780036389.01.T01 | MTR_4g017830 | 34.483 | 522 | 262 | 16 | 6 | 465 | 4 | 507 | 1.37e-71 | 236 |
| MsG0780036389.01.T01 | MTR_4g017830 | 28.571 | 266 | 155 | 8 | 2 | 242 | 255 | 510 | 2.05e-16 | 82.0 |
| MsG0780036389.01.T01 | MTR_4g019340 | 35.818 | 483 | 240 | 14 | 15 | 447 | 39 | 501 | 2.13e-70 | 232 |
| MsG0780036389.01.T01 | MTR_4g019340 | 27.074 | 229 | 139 | 7 | 2 | 219 | 291 | 502 | 4.29e-15 | 77.8 |
| MsG0780036389.01.T01 | MTR_4g017440 | 33.781 | 521 | 276 | 17 | 1 | 465 | 1 | 508 | 8.70e-69 | 228 |
| MsG0780036389.01.T01 | MTR_4g017410 | 34.980 | 506 | 263 | 17 | 8 | 465 | 3 | 490 | 1.41e-67 | 224 |
| MsG0780036389.01.T01 | MTR_4g017610 | 34.433 | 485 | 245 | 16 | 10 | 447 | 9 | 467 | 4.34e-67 | 223 |
| MsG0780036389.01.T01 | MTR_4g017550 | 33.689 | 469 | 244 | 17 | 41 | 457 | 18 | 471 | 1.27e-58 | 200 |
| MsG0780036389.01.T01 | MTR_4g017550 | 31.120 | 241 | 116 | 8 | 14 | 224 | 247 | 467 | 1.16e-16 | 82.4 |
| MsG0780036389.01.T01 | MTR_4g017470 | 32.816 | 515 | 241 | 17 | 10 | 465 | 8 | 476 | 1.40e-57 | 198 |
| MsG0780036389.01.T01 | MTR_4g017470 | 26.506 | 249 | 143 | 8 | 6 | 224 | 226 | 464 | 1.51e-13 | 73.2 |
| MsG0780036389.01.T01 | MTR_7g062270 | 47.093 | 172 | 84 | 5 | 15 | 183 | 11 | 178 | 1.87e-41 | 152 |
| MsG0780036389.01.T01 | MTR_7g063720 | 38.679 | 212 | 128 | 2 | 256 | 466 | 23 | 233 | 2.29e-37 | 137 |
| MsG0780036389.01.T01 | MTR_7g063720 | 25.688 | 218 | 135 | 6 | 14 | 224 | 23 | 220 | 2.96e-11 | 63.9 |
| MsG0780036389.01.T01 | MTR_2g067310 | 45.665 | 173 | 54 | 3 | 25 | 197 | 58 | 190 | 1.01e-34 | 130 |
| MsG0780036389.01.T01 | MTR_8g023240 | 26.638 | 473 | 282 | 17 | 15 | 451 | 38 | 481 | 2.47e-31 | 127 |
| MsG0780036389.01.T01 | MTR_8g445860 | 33.761 | 234 | 128 | 6 | 14 | 229 | 13 | 237 | 2.88e-30 | 118 |
| MsG0780036389.01.T01 | MTR_8g445860 | 28.182 | 220 | 132 | 5 | 256 | 451 | 13 | 230 | 2.16e-17 | 81.6 |
| MsG0780036389.01.T01 | MTR_0150s0010 | 32.932 | 249 | 127 | 9 | 8 | 230 | 4 | 238 | 1.06e-28 | 113 |
| MsG0780036389.01.T01 | MTR_1g115440 | 33.898 | 236 | 132 | 6 | 13 | 230 | 10 | 239 | 9.25e-27 | 108 |
| MsG0780036389.01.T01 | MTR_1g115440 | 28.571 | 224 | 131 | 6 | 255 | 451 | 10 | 231 | 8.38e-17 | 80.1 |
| MsG0780036389.01.T01 | MTR_7g063690 | 32.302 | 291 | 118 | 10 | 1 | 283 | 3 | 222 | 1.41e-25 | 105 |
| MsG0780036389.01.T01 | MTR_1g115455 | 31.579 | 247 | 127 | 10 | 8 | 229 | 3 | 232 | 1.63e-21 | 93.6 |
| MsG0780036389.01.T01 | MTR_4g019240 | 39.535 | 129 | 68 | 4 | 3 | 123 | 2 | 128 | 6.29e-21 | 90.5 |
| MsG0780036389.01.T01 | MTR_7g066390 | 37.278 | 169 | 65 | 2 | 297 | 465 | 1 | 128 | 9.44e-21 | 89.0 |
| MsG0780036389.01.T01 | MTR_7g100420 | 31.329 | 316 | 183 | 10 | 166 | 459 | 94 | 397 | 7.13e-19 | 89.0 |
| MsG0780036389.01.T01 | MTR_7g100420 | 54.545 | 77 | 29 | 2 | 15 | 85 | 40 | 116 | 7.16e-14 | 73.6 |
| MsG0780036389.01.T01 | MTR_1g115450 | 43.529 | 85 | 43 | 1 | 14 | 98 | 7 | 86 | 5.97e-17 | 77.8 |
| MsG0780036389.01.T01 | MTR_0150s0030 | 41.758 | 91 | 47 | 2 | 8 | 97 | 2 | 87 | 1.25e-15 | 75.9 |
| MsG0780036389.01.T01 | MTR_8g445910 | 41.758 | 91 | 47 | 2 | 8 | 97 | 2 | 87 | 1.40e-14 | 74.3 |
| MsG0780036389.01.T01 | MTR_8g044880 | 41.758 | 91 | 47 | 2 | 8 | 97 | 2 | 87 | 1.74e-14 | 73.9 |
| MsG0780036389.01.T01 | MTR_1g115435 | 28.879 | 232 | 123 | 9 | 14 | 231 | 7 | 210 | 2.28e-13 | 69.7 |
| MsG0780036389.01.T01 | MTR_8g445920 | 38.824 | 85 | 47 | 1 | 8 | 92 | 4 | 83 | 4.76e-13 | 65.9 |
| MsG0780036389.01.T01 | MTR_8g044890 | 38.824 | 85 | 47 | 1 | 8 | 92 | 4 | 83 | 4.76e-13 | 65.9 |
| MsG0780036389.01.T01 | MTR_7g063870 | 56.364 | 55 | 24 | 0 | 8 | 62 | 25 | 79 | 8.23e-13 | 68.9 |
| MsG0780036389.01.T01 | MTR_1g115470 | 37.113 | 97 | 54 | 2 | 14 | 108 | 6 | 97 | 1.56e-12 | 69.7 |
| MsG0780036389.01.T01 | MTR_4g019330 | 28.415 | 183 | 89 | 3 | 270 | 451 | 32 | 173 | 1.86e-12 | 66.2 |
| MsG0780036389.01.T01 | MTR_7g063760 | 43.662 | 71 | 39 | 1 | 397 | 466 | 4 | 74 | 8.34e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036389.01.T01 | AT5G01130 | 32.538 | 461 | 271 | 9 | 14 | 459 | 9 | 444 | 5.62e-59 | 201 |
| MsG0780036389.01.T01 | AT3G09110 | 37.117 | 326 | 184 | 8 | 6 | 318 | 2 | 319 | 2.09e-57 | 193 |
| MsG0780036389.01.T01 | AT5G01120 | 30.962 | 478 | 291 | 11 | 15 | 455 | 10 | 485 | 4.69e-56 | 194 |
| MsG0780036389.01.T01 | AT3G09140 | 31.727 | 498 | 259 | 16 | 14 | 451 | 9 | 485 | 1.54e-53 | 187 |
| MsG0780036389.01.T01 | AT5G01150 | 30.722 | 485 | 283 | 11 | 14 | 459 | 9 | 479 | 3.84e-53 | 186 |
| MsG0780036389.01.T01 | AT3G09140 | 30.228 | 526 | 258 | 16 | 14 | 451 | 9 | 513 | 5.60e-50 | 178 |
| MsG0780036389.01.T01 | AT5G43240 | 29.459 | 499 | 275 | 14 | 15 | 455 | 10 | 489 | 1.02e-48 | 174 |
| MsG0780036389.01.T01 | AT5G43240 | 29.459 | 499 | 275 | 14 | 15 | 455 | 10 | 489 | 1.02e-48 | 174 |
| MsG0780036389.01.T01 | AT5G43240 | 29.459 | 499 | 275 | 14 | 15 | 455 | 10 | 489 | 1.02e-48 | 174 |
| MsG0780036389.01.T01 | AT3G09120 | 34.921 | 315 | 182 | 8 | 13 | 310 | 5 | 313 | 4.54e-47 | 165 |
| MsG0780036389.01.T01 | AT5G01140 | 29.918 | 488 | 255 | 12 | 14 | 459 | 9 | 451 | 1.88e-46 | 167 |
| MsG0780036389.01.T01 | AT5G37320 | 27.198 | 489 | 285 | 20 | 15 | 455 | 10 | 475 | 4.50e-32 | 128 |
| MsG0780036389.01.T01 | AT5G43240 | 26.590 | 346 | 187 | 11 | 159 | 455 | 18 | 345 | 3.81e-14 | 74.3 |
Find 67 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATTCTCACGATAAAAT+TGG | 0.210786 | 7:-7440255 | MsG0780036389.01.T01:CDS |
| TCAACATCTGCTTTGCAATA+TGG | 0.234722 | 7:-7439895 | MsG0780036389.01.T01:CDS |
| CTATATTTAGTTTGAGCTTC+TGG | 0.242708 | 7:+7441169 | None:intergenic |
| TAATCTTCCATCGAGTTTCT+AGG | 0.245861 | 7:+7441195 | None:intergenic |
| TCCGAACCGAAGAAACAATT+TGG | 0.269005 | 7:-7440688 | MsG0780036389.01.T01:CDS |
| GAAGAAAGAGTTATCAAAAT+TGG | 0.297409 | 7:-7440047 | MsG0780036389.01.T01:CDS |
| AACAATTTGGAGTTTCTGAT+TGG | 0.320877 | 7:-7440675 | MsG0780036389.01.T01:CDS |
| ATTGAGCAGCATTTCTTTGC+AGG | 0.322996 | 7:+7441218 | None:intergenic |
| TAAGTATGACAATACTTTAT+AGG | 0.329214 | 7:+7440327 | None:intergenic |
| TCCAAATTGTTTCTTCGGTT+CGG | 0.334477 | 7:+7440687 | None:intergenic |
| GTTCTTCTTTAAAATAAAAC+TGG | 0.335816 | 7:+7440725 | None:intergenic |
| AAAGTTTGTACCATTAGAAT+TGG | 0.341082 | 7:-7440223 | MsG0780036389.01.T01:CDS |
| CAAAGCAGCCTTTGAGGTTA+AGG | 0.345875 | 7:+7440749 | None:intergenic |
| TGCGACATTAAATATATATC+TGG | 0.357846 | 7:+7440438 | None:intergenic |
| GAAACTCCAAATTGTTTCTT+CGG | 0.358111 | 7:+7440682 | None:intergenic |
| TTGTCATTTCTCCACCTTAA+CGG | 0.368739 | 7:+7439852 | None:intergenic |
| AATATTGAAGCAGTGAGATT+TGG | 0.383738 | 7:-7441303 | MsG0780036389.01.T01:CDS |
| TATTGCAAGATTAATGTCTA+AGG | 0.384286 | 7:-7441331 | MsG0780036389.01.T01:CDS |
| CAAATTCTGTTTGTTGAAGC+CGG | 0.398212 | 7:-7440588 | MsG0780036389.01.T01:CDS |
| AACTTGACCAACAATATCTA+TGG | 0.419276 | 7:-7441248 | MsG0780036389.01.T01:CDS |
| CACTGATCAGCCAATTCTAA+TGG | 0.421900 | 7:+7440213 | None:intergenic |
| GAAATTTATAAGAACCGTTA+AGG | 0.430047 | 7:-7439866 | MsG0780036389.01.T01:CDS |
| ATCGGTAAGAGTTGGTTCAT+TGG | 0.433957 | 7:+7440366 | None:intergenic |
| CTTTCTTCCAAGTCAGATAG+AGG | 0.443297 | 7:+7440062 | None:intergenic |
| AGCTACCGTCTGACGACAAT+AGG | 0.447310 | 7:-7440650 | MsG0780036389.01.T01:CDS |
| TTCTAACCTTCCCCTTAGGA+GGG | 0.463532 | 7:-7440536 | MsG0780036389.01.T01:CDS |
| TTTCTAACCTTCCCCTTAGG+AGG | 0.466529 | 7:-7440537 | MsG0780036389.01.T01:CDS |
| ACTGGTCAAAGCAGCCTTTG+AGG | 0.467868 | 7:+7440743 | None:intergenic |
| TTACGTCTGCTGCAAAAGCT+TGG | 0.475407 | 7:-7440919 | MsG0780036389.01.T01:CDS |
| TCAAATTGCGATGCGATAAG+AGG | 0.478068 | 7:+7440390 | None:intergenic |
| AGATATATATTTAATGTCGC+AGG | 0.479517 | 7:-7440436 | MsG0780036389.01.T01:CDS |
| TCTGTATGCTGAAGCTGGTA+AGG | 0.484594 | 7:-7441400 | MsG0780036389.01.T01:CDS |
| AAATTCTGTTTGTTGAAGCC+GGG | 0.488713 | 7:-7440587 | MsG0780036389.01.T01:CDS |
| GAATATTCAAACCTAGAAAA+CGG | 0.489274 | 7:-7441015 | MsG0780036389.01.T01:CDS |
| ACTTACTATGATCACAAAAG+TGG | 0.494646 | 7:-7440309 | MsG0780036389.01.T01:CDS |
| CATGTGTAAAACCCCTCCTA+AGG | 0.499269 | 7:+7440524 | None:intergenic |
| AAAAGTTCCTCTATCTGACT+TGG | 0.514582 | 7:-7440069 | MsG0780036389.01.T01:CDS |
| GGTTCGGATTAACAACAAAT+CGG | 0.521316 | 7:+7440703 | None:intergenic |
| CAAGTTATCCTTAACCTCAA+AGG | 0.525899 | 7:-7440757 | MsG0780036389.01.T01:CDS |
| GGTAAGGTAGCTGCACCAAT+CGG | 0.526064 | 7:+7440348 | None:intergenic |
| AGTTTCTTAACATTGCCTCT+AGG | 0.529178 | 7:-7441357 | MsG0780036389.01.T01:CDS |
| TCTAACCTTCCCCTTAGGAG+GGG | 0.532923 | 7:-7440535 | MsG0780036389.01.T01:CDS |
| GAATTGGGATTGCAGAAGAA+AGG | 0.538556 | 7:-7441115 | MsG0780036389.01.T01:CDS |
| ATGACAATACTTTATAGGTA+AGG | 0.538944 | 7:+7440332 | None:intergenic |
| AGTTATCAAAATTGGTGTGA+AGG | 0.549919 | 7:-7440039 | MsG0780036389.01.T01:CDS |
| AGAGTTCTGTATGCTGAAGC+TGG | 0.551876 | 7:-7441405 | MsG0780036389.01.T01:CDS |
| TATGTGACCATAGATATTGT+TGG | 0.554667 | 7:+7441241 | None:intergenic |
| ATGTGTAAAACCCCTCCTAA+GGG | 0.556431 | 7:+7440525 | None:intergenic |
| TATCAAAATTGGTGTGAAGG+AGG | 0.560634 | 7:-7440036 | MsG0780036389.01.T01:intron |
| ATGAACCAACTCTTACCGAT+TGG | 0.580595 | 7:-7440363 | MsG0780036389.01.T01:CDS |
| ATTTATAAGAACCGTTAAGG+TGG | 0.581384 | 7:-7439863 | MsG0780036389.01.T01:CDS |
| CTGCACCAATCGGTAAGAGT+TGG | 0.587039 | 7:+7440358 | None:intergenic |
| AAAGTCAATAAAATCATCCC+CGG | 0.589690 | 7:+7440569 | None:intergenic |
| GATATATATTTAATGTCGCA+GGG | 0.593064 | 7:-7440435 | MsG0780036389.01.T01:CDS |
| CTTACTATGATCACAAAAGT+GGG | 0.603766 | 7:-7440308 | MsG0780036389.01.T01:CDS |
| GCTCAATCCTAGAAACTCGA+TGG | 0.610851 | 7:-7441202 | MsG0780036389.01.T01:CDS |
| TAAAACCCCTCCTAAGGGGA+AGG | 0.611425 | 7:+7440530 | None:intergenic |
| CCGCACACAAAATACTGCAT+AGG | 0.614576 | 7:+7441138 | None:intergenic |
| AATTCTGTTTGTTGAAGCCG+GGG | 0.617462 | 7:-7440586 | MsG0780036389.01.T01:CDS |
| TTTCTAGGTTTGAATATTCG+GGG | 0.622034 | 7:+7441019 | None:intergenic |
| ATCTGCCTATTGTCGTCAGA+CGG | 0.629290 | 7:+7440645 | None:intergenic |
| TATCAATCTTACAAAATCCG+CGG | 0.645310 | 7:-7440277 | MsG0780036389.01.T01:CDS |
| ATATTGCAAAGCAGATGTTG+AGG | 0.659281 | 7:+7439897 | None:intergenic |
| TGTGTAAAACCCCTCCTAAG+GGG | 0.668372 | 7:+7440526 | None:intergenic |
| AATCTTGCAATAGTACCTAG+AGG | 0.704960 | 7:+7441342 | None:intergenic |
| GATTGCAGAAGAAAGGACAG+TGG | 0.732973 | 7:-7441108 | MsG0780036389.01.T01:CDS |
| TTTATCGTGAGAATAATCCG+CGG | 0.765639 | 7:+7440260 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTATTCATTAAATGTTACA+GGG | - | Chr7:7441392-7441411 | MsG0780036389.01.T01:CDS | 20.0% |
| !! | TAAGTATGACAATACTTTAT+AGG | + | Chr7:7441014-7441033 | None:intergenic | 20.0% |
| !!! | CTCTAATTGATTATTTCTAT+AGG | - | Chr7:7440545-7440564 | MsG0780036389.01.T01:CDS | 20.0% |
| !!! | GTTCTTCTTTAAAATAAAAC+TGG | + | Chr7:7440616-7440635 | None:intergenic | 20.0% |
| !!! | TTGTTCATTTGATTTTCTTA+CGG | + | Chr7:7440733-7440752 | None:intergenic | 20.0% |
| ! | AAAGTTTGTACCATTAGAAT+TGG | - | Chr7:7441115-7441134 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | AGATATATATTTAATGTCGC+AGG | - | Chr7:7440902-7440921 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | ATGACAATACTTTATAGGTA+AGG | + | Chr7:7441009-7441028 | None:intergenic | 25.0% |
| ! | GAAATTTATAAGAACCGTTA+AGG | - | Chr7:7441472-7441491 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | GAAGAAAGAGTTATCAAAAT+TGG | - | Chr7:7441291-7441310 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | GAATATTCAAACCTAGAAAA+CGG | - | Chr7:7440323-7440342 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | GATATATATTTAATGTCGCA+GGG | - | Chr7:7440903-7440922 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | GATTATTCTCACGATAAAAT+TGG | - | Chr7:7441083-7441102 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | GCTTATTCATTAAATGTTAC+AGG | - | Chr7:7441391-7441410 | MsG0780036389.01.T01:CDS | 25.0% |
| ! | TATTGCAAGATTAATGTCTA+AGG | - | Chr7:7440007-7440026 | MsG0780036389.01.T01:intron | 25.0% |
| ! | TGCGACATTAAATATATATC+TGG | + | Chr7:7440903-7440922 | None:intergenic | 25.0% |
| !! | AAATGATGATTTTGCAATAG+AGG | + | Chr7:7441160-7441179 | None:intergenic | 25.0% |
| !! | GCAAAATCATCATTTGTTAA+AGG | - | Chr7:7441165-7441184 | MsG0780036389.01.T01:CDS | 25.0% |
| !! | GTTTTCTAGGTTTGAATATT+CGG | + | Chr7:7440324-7440343 | None:intergenic | 25.0% |
| !! | TTTTCTAGGTTTGAATATTC+GGG | + | Chr7:7440323-7440342 | None:intergenic | 25.0% |
| AAAGTCAATAAAATCATCCC+CGG | + | Chr7:7440772-7440791 | None:intergenic | 30.0% | |
| AACTTGACCAACAATATCTA+TGG | - | Chr7:7440090-7440109 | MsG0780036389.01.T01:CDS | 30.0% | |
| AATATTGAAGCAGTGAGATT+TGG | - | Chr7:7440035-7440054 | MsG0780036389.01.T01:intron | 30.0% | |
| ACTTACTATGATCACAAAAG+TGG | - | Chr7:7441029-7441048 | MsG0780036389.01.T01:CDS | 30.0% | |
| AGGAACAAAAAATGCTATTG+TGG | - | Chr7:7440272-7440291 | MsG0780036389.01.T01:CDS | 30.0% | |
| AGTTATCAAAATTGGTGTGA+AGG | - | Chr7:7441299-7441318 | MsG0780036389.01.T01:CDS | 30.0% | |
| ATTTATAAGAACCGTTAAGG+TGG | - | Chr7:7441475-7441494 | MsG0780036389.01.T01:CDS | 30.0% | |
| CTTACTATGATCACAAAAGT+GGG | - | Chr7:7441030-7441049 | MsG0780036389.01.T01:CDS | 30.0% | |
| GGAACAAAAAATGCTATTGT+GGG | - | Chr7:7440273-7440292 | MsG0780036389.01.T01:CDS | 30.0% | |
| TATCAATCTTACAAAATCCG+CGG | - | Chr7:7441061-7441080 | MsG0780036389.01.T01:CDS | 30.0% | |
| TATGTGACCATAGATATTGT+TGG | + | Chr7:7440100-7440119 | None:intergenic | 30.0% | |
| TTTCTAGGTTTGAATATTCG+GGG | + | Chr7:7440322-7440341 | None:intergenic | 30.0% | |
| ! | AACAATTTGGAGTTTCTGAT+TGG | - | Chr7:7440663-7440682 | MsG0780036389.01.T01:CDS | 30.0% |
| ! | GAAACTCCAAATTGTTTCTT+CGG | + | Chr7:7440659-7440678 | None:intergenic | 30.0% |
| ! | TCTAATGGTACAAACTTTTC+TGG | + | Chr7:7441113-7441132 | None:intergenic | 30.0% |
| !! | CTATATTTAGTTTGAGCTTC+TGG | + | Chr7:7440172-7440191 | None:intergenic | 30.0% |
| AAAAGTTCCTCTATCTGACT+TGG | - | Chr7:7441269-7441288 | MsG0780036389.01.T01:CDS | 35.0% | |
| AAACTTGCCCCAAAAACATT+AGG | + | Chr7:7440401-7440420 | None:intergenic | 35.0% | |
| AATCTTGCAATAGTACCTAG+AGG | + | Chr7:7439999-7440018 | None:intergenic | 35.0% | |
| AGTTTCTTAACATTGCCTCT+AGG | - | Chr7:7439981-7440000 | MsG0780036389.01.T01:intron | 35.0% | |
| ATATTGCAAAGCAGATGTTG+AGG | + | Chr7:7441444-7441463 | None:intergenic | 35.0% | |
| CAAGTTATCCTTAACCTCAA+AGG | - | Chr7:7440581-7440600 | MsG0780036389.01.T01:CDS | 35.0% | |
| CTCTTTCTTGTCGATATCAA+CGG | + | Chr7:7440477-7440496 | None:intergenic | 35.0% | |
| GGTTCGGATTAACAACAAAT+CGG | + | Chr7:7440638-7440657 | None:intergenic | 35.0% | |
| TAAAGGACCATCAACTTACA+TGG | - | Chr7:7441182-7441201 | MsG0780036389.01.T01:CDS | 35.0% | |
| TAATCTTCCATCGAGTTTCT+AGG | + | Chr7:7440146-7440165 | None:intergenic | 35.0% | |
| TATCAAAATTGGTGTGAAGG+AGG | - | Chr7:7441302-7441321 | MsG0780036389.01.T01:CDS | 35.0% | |
| TCAACATCTGCTTTGCAATA+TGG | - | Chr7:7441443-7441462 | MsG0780036389.01.T01:CDS | 35.0% | |
| TTTATCGTGAGAATAATCCG+CGG | + | Chr7:7441081-7441100 | None:intergenic | 35.0% | |
| ! | AAATTCTGTTTGTTGAAGCC+GGG | - | Chr7:7440751-7440770 | MsG0780036389.01.T01:CDS | 35.0% |
| ! | ACGTGATGCCTAATGTTTTT+GGG | - | Chr7:7440390-7440409 | MsG0780036389.01.T01:CDS | 35.0% |
| ! | CAAATTCTGTTTGTTGAAGC+CGG | - | Chr7:7440750-7440769 | MsG0780036389.01.T01:CDS | 35.0% |
| ! | CCTTAACAAAACCGTTTTCT+AGG | + | Chr7:7440337-7440356 | None:intergenic | 35.0% |
| ! | TACGTGATGCCTAATGTTTT+TGG | - | Chr7:7440389-7440408 | MsG0780036389.01.T01:CDS | 35.0% |
| ! | TCCAAATTGTTTCTTCGGTT+CGG | + | Chr7:7440654-7440673 | None:intergenic | 35.0% |
| !! | GAGTTTTAGTCGACAAAGAA+AGG | - | Chr7:7439907-7439926 | MsG0780036389.01.T01:CDS | 35.0% |
| !! | GATGTCGTTTGAGCATTTTT+AGG | - | Chr7:7440252-7440271 | MsG0780036389.01.T01:CDS | 35.0% |
| !!! | CCTAGAAAACGGTTTTGTTA+AGG | - | Chr7:7440334-7440353 | MsG0780036389.01.T01:CDS | 35.0% |
| ATGAACCAACTCTTACCGAT+TGG | - | Chr7:7440975-7440994 | MsG0780036389.01.T01:CDS | 40.0% | |
| ATGTGTAAAACCCCTCCTAA+GGG | + | Chr7:7440816-7440835 | None:intergenic | 40.0% | |
| ATTGAGCAGCATTTCTTTGC+AGG | + | Chr7:7440123-7440142 | None:intergenic | 40.0% | |
| CACTGATCAGCCAATTCTAA+TGG | + | Chr7:7441128-7441147 | None:intergenic | 40.0% | |
| CGTTGATATCGACAAGAAAG+AGG | - | Chr7:7440475-7440494 | MsG0780036389.01.T01:CDS | 40.0% | |
| CTTTCTTCCAAGTCAGATAG+AGG | + | Chr7:7441279-7441298 | None:intergenic | 40.0% | |
| GAATTGGGATTGCAGAAGAA+AGG | - | Chr7:7440223-7440242 | MsG0780036389.01.T01:CDS | 40.0% | |
| TCAAATTGCGATGCGATAAG+AGG | + | Chr7:7440951-7440970 | None:intergenic | 40.0% | |
| TCCGAACCGAAGAAACAATT+TGG | - | Chr7:7440650-7440669 | MsG0780036389.01.T01:CDS | 40.0% | |
| ! | AATTCTGTTTGTTGAAGCCG+GGG | - | Chr7:7440752-7440771 | MsG0780036389.01.T01:CDS | 40.0% |
| ! | AGTATTTTGTGTGCGGGAAT+TGG | - | Chr7:7440207-7440226 | MsG0780036389.01.T01:intron | 40.0% |
| ! | AGTTTTCTAACCTTCCCCTT+AGG | - | Chr7:7440798-7440817 | MsG0780036389.01.T01:intron | 40.0% |
| ! | ATCGGTAAGAGTTGGTTCAT+TGG | + | Chr7:7440975-7440994 | None:intergenic | 40.0% |
| ! | CCTATGCAGTATTTTGTGTG+CGG | - | Chr7:7440200-7440219 | MsG0780036389.01.T01:intron | 40.0% |
| ! | CTATGCAGTATTTTGTGTGC+GGG | - | Chr7:7440201-7440220 | MsG0780036389.01.T01:intron | 40.0% |
| ! | GTATTTTGTGTGCGGGAATT+GGG | - | Chr7:7440208-7440227 | MsG0780036389.01.T01:intron | 40.0% |
| ! | TCAGTCACCATGTAAGTTGA+TGG | + | Chr7:7441192-7441211 | None:intergenic | 40.0% |
| !! | CGTGATGCCTAATGTTTTTG+GGG | - | Chr7:7440391-7440410 | MsG0780036389.01.T01:CDS | 40.0% |
| AGAGTTCTGTATGCTGAAGC+TGG | - | Chr7:7439933-7439952 | MsG0780036389.01.T01:intron | 45.0% | |
| ATCTGCCTATTGTCGTCAGA+CGG | + | Chr7:7440696-7440715 | None:intergenic | 45.0% | |
| CATGTGTAAAACCCCTCCTA+AGG | + | Chr7:7440817-7440836 | None:intergenic | 45.0% | |
| CCGCACACAAAATACTGCAT+AGG | + | Chr7:7440203-7440222 | None:intergenic | 45.0% | |
| GATTGCAGAAGAAAGGACAG+TGG | - | Chr7:7440230-7440249 | MsG0780036389.01.T01:CDS | 45.0% | |
| GCTCAATCCTAGAAACTCGA+TGG | - | Chr7:7440136-7440155 | MsG0780036389.01.T01:intron | 45.0% | |
| TCTGTATGCTGAAGCTGGTA+AGG | - | Chr7:7439938-7439957 | MsG0780036389.01.T01:intron | 45.0% | |
| TGTGTAAAACCCCTCCTAAG+GGG | + | Chr7:7440815-7440834 | None:intergenic | 45.0% | |
| TTACGTCTGCTGCAAAAGCT+TGG | - | Chr7:7440419-7440438 | MsG0780036389.01.T01:CDS | 45.0% | |
| TTCTAACCTTCCCCTTAGGA+GGG | - | Chr7:7440802-7440821 | MsG0780036389.01.T01:intron | 45.0% | |
| TTTCTAACCTTCCCCTTAGG+AGG | - | Chr7:7440801-7440820 | MsG0780036389.01.T01:intron | 45.0% | |
| ! | GGGGTTTTACACATGCTTCA+AGG | - | Chr7:7440822-7440841 | MsG0780036389.01.T01:intron | 45.0% |
| !! | CAAAGCAGCCTTTGAGGTTA+AGG | + | Chr7:7440592-7440611 | None:intergenic | 45.0% |
| ACTGGTCAAAGCAGCCTTTG+AGG | + | Chr7:7440598-7440617 | None:intergenic | 50.0% | |
| AGCTACCGTCTGACGACAAT+AGG | - | Chr7:7440688-7440707 | MsG0780036389.01.T01:CDS | 50.0% | |
| CTGCACCAATCGGTAAGAGT+TGG | + | Chr7:7440983-7441002 | None:intergenic | 50.0% | |
| GGTAAGGTAGCTGCACCAAT+CGG | + | Chr7:7440993-7441012 | None:intergenic | 50.0% | |
| TAAAACCCCTCCTAAGGGGA+AGG | + | Chr7:7440811-7440830 | None:intergenic | 50.0% | |
| TCTAACCTTCCCCTTAGGAG+GGG | - | Chr7:7440803-7440822 | MsG0780036389.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 7439855 | 7441505 | 7439855 | ID=MsG0780036389.01;Name=MsG0780036389.01 |
| Chr7 | mRNA | 7439855 | 7441505 | 7439855 | ID=MsG0780036389.01.T01;Parent=MsG0780036389.01;Name=MsG0780036389.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|4|0|466 |
| Chr7 | exon | 7440885 | 7441505 | 7440885 | ID=MsG0780036389.01.T01:exon:3943;Parent=MsG0780036389.01.T01 |
| Chr7 | exon | 7440216 | 7440793 | 7440216 | ID=MsG0780036389.01.T01:exon:3942;Parent=MsG0780036389.01.T01 |
| Chr7 | exon | 7440037 | 7440145 | 7440037 | ID=MsG0780036389.01.T01:exon:3941;Parent=MsG0780036389.01.T01 |
| Chr7 | exon | 7439855 | 7439947 | 7439855 | ID=MsG0780036389.01.T01:exon:3940;Parent=MsG0780036389.01.T01 |
| Chr7 | CDS | 7440885 | 7441505 | 7440885 | ID=MsG0780036389.01.T01:cds;Parent=MsG0780036389.01.T01 |
| Chr7 | CDS | 7440216 | 7440793 | 7440216 | ID=MsG0780036389.01.T01:cds;Parent=MsG0780036389.01.T01 |
| Chr7 | CDS | 7440037 | 7440145 | 7440037 | ID=MsG0780036389.01.T01:cds;Parent=MsG0780036389.01.T01 |
| Chr7 | CDS | 7439855 | 7439947 | 7439855 | ID=MsG0780036389.01.T01:cds;Parent=MsG0780036389.01.T01 |
| Gene Sequence |
| Protein sequence |
