AlfalfaGEDB | MsG0480018721.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018721.01

KEGG: -
GO: -
PFAM: PF05056

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018721.01.T01	AES86950.1	68.826	494	114	5	207	691	4	466	0	646

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018721.01.T01	G7JQ74	68.826	494	114	5	207	691	4	466	0.0	646

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0480018545.01	MsG0480018721.01	PPI
MsG0480018548.01	MsG0480018721.01	PPI
MsG0480018550.01	MsG0480018721.01	PPI
MsG0480018551.01	MsG0480018721.01	PPI
MsG0480018553.01	MsG0480018721.01	PPI
MsG0480018554.01	MsG0480018721.01	PPI
MsG0480018558.01	MsG0480018721.01	PPI
MsG0480018719.01	MsG0480018721.01	PPI
MsG0480018720.01	MsG0480018721.01	PPI
MsG0480018721.01	MsG0480018857.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018721.01.T01	MTR_4g017410	68.826	494	114	5	207	691	4	466	0.0	646
MsG0480018721.01.T01	MTR_4g017440	64.040	495	149	6	203	687	5	480	0.0	596
MsG0480018721.01.T01	MTR_4g017830	61.155	502	163	8	202	691	2	483	0.0	587
MsG0480018721.01.T01	MTR_4g017610	59.438	498	159	9	200	691	1	461	0.0	546
MsG0480018721.01.T01	MTR_4g017550	64.783	460	133	10	239	691	18	455	0.0	538
MsG0480018721.01.T01	MTR_4g019340	59.719	499	143	9	211	691	37	495	0.0	534
MsG0480018721.01.T01	MTR_4g017470	58.954	497	146	8	200	687	1	448	1.07e-179	521
MsG0480018721.01.T01	MTR_4g019310	38.521	514	251	14	200	691	1	471	3.50e-98	311
MsG0480018721.01.T01	MTR_7g058710	31.650	515	263	12	200	686	1	454	1.13e-61	214
MsG0480018721.01.T01	MTR_7g066350	38.043	368	196	11	206	555	3	356	6.97e-61	211

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018721.01.T01	AT5G01130	30.677	502	254	16	206	691	3	426	5.08e-54	192
MsG0480018721.01.T01	AT5G01120	29.960	504	294	14	209	691	6	471	2.44e-50	184
MsG0480018721.01.T01	AT5G01150	29.980	507	286	16	206	691	3	461	2.32e-46	172
MsG0480018721.01.T01	AT3G09140	29.094	519	289	19	206	691	3	475	4.75e-46	171
MsG0480018721.01.T01	AT5G43240	30.859	512	275	18	213	691	10	475	6.69e-46	171
MsG0480018721.01.T01	AT5G43240	30.859	512	275	18	213	691	10	475	6.69e-46	171
MsG0480018721.01.T01	AT5G43240	30.859	512	275	18	213	691	10	475	6.69e-46	171
MsG0480018721.01.T01	AT3G09110	35.435	333	186	11	209	531	6	319	2.37e-45	165
MsG0480018721.01.T01	AT5G01140	29.940	501	248	18	215	691	12	433	3.71e-44	166
MsG0480018721.01.T01	AT3G09120	33.531	337	176	11	209	523	3	313	1.17e-41	154

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Find 132 sgRNAs with CRISPR-Local

Find 622 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACGATAGAGGATGTTG+CGG	0.627800	4:+8958953	MsG0480018721.01.T01:CDS
AAATCAACAGCCTCGAAATT+TGG	0.164742	4:+8962086	MsG0480018721.01.T01:CDS
AACAAATGTTGTTGTATCCC+AGG	0.489709	4:+8961926	MsG0480018721.01.T01:CDS
AAGAGGGTTGTTGACAGTTA+TGG	0.391241	4:+8958605	MsG0480018721.01.T01:CDS
AAGAGTTCTACTTGCTGACA+TGG	0.574763	4:+8961609	MsG0480018721.01.T01:CDS
AAGGAATGGTGATGATTCTG+AGG	0.543097	4:-8961830	None:intergenic
AAGTAAGTCACAGACGAACA+TGG	0.633871	4:-8962192	None:intergenic
AATAGTGCGGAAGCCCAAAA+TGG	0.402429	4:+8962132	MsG0480018721.01.T01:CDS
AATTATGTAACAAATCAACA+TGG	0.693917	4:-8958782	None:intergenic
ACAAAATGGTCTCGCAAGTA+TGG	0.308598	4:-8958684	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGAATGGTTCAAATTGA+AGG	-	Chr4:8959741-8959760	None:intergenic	30.0%
AAATTGCAATAACATCACTC+AGG	-	Chr4:8953808-8953827	None:intergenic	30.0%
AACCCTTTCCTTTATGATTA+GGG	-	Chr4:8958041-8958060	None:intergenic	30.0%
AAGGACTTGTATAATTACAC+TGG	-	Chr4:8959768-8959787	None:intergenic	30.0%
AAGTAGCCTATGATTTCATT+CGG	+	Chr4:8957788-8957807	MsG0480018721.01.T01:intron	30.0%
AATTATTAGGGATTTGAGAG+AGG	-	Chr4:8960528-8960547	None:intergenic	30.0%
AATTGCCTATGTGTTTGTAA+AGG	-	Chr4:8954559-8954578	None:intergenic	30.0%
AGAAAGATTTGACCAATGAA+AGG	-	Chr4:8955173-8955192	None:intergenic	30.0%
AGGTAATTAATTCTGTTGTG+TGG	+	Chr4:8962289-8962308	MsG0480018721.01.T01:intron	30.0%
AGTCTCCTCAACAAAATTAA+TGG	-	Chr4:8957186-8957205	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	8953763	8963734	8953763	ID=MsG0480018721.01;Name=MsG0480018721.01
Chr4	mRNA	8953763	8963734	8953763	ID=MsG0480018721.01.T01;Parent=MsG0480018721.01;Name=MsG0480018721.01.T01;_AED=0.46;_eAED=0.47;_QI=0\|0\|0\|0.8\|0.5\|0.4\|5\|0\|694
Chr4	exon	8953763	8953777	8953763	ID=MsG0480018721.01.T01:exon:2868;Parent=MsG0480018721.01.T01
Chr4	exon	8953977	8954086	8953977	ID=MsG0480018721.01.T01:exon:2869;Parent=MsG0480018721.01.T01
Chr4	exon	8958590	8958974	8958590	ID=MsG0480018721.01.T01:exon:2870;Parent=MsG0480018721.01.T01
Chr4	exon	8961541	8962290	8961541	ID=MsG0480018721.01.T01:exon:2871;Parent=MsG0480018721.01.T01
Chr4	exon	8962910	8963734	8962910	ID=MsG0480018721.01.T01:exon:2872;Parent=MsG0480018721.01.T01
Chr4	CDS	8953763	8953777	8953763	ID=MsG0480018721.01.T01:cds;Parent=MsG0480018721.01.T01
Chr4	CDS	8953977	8954086	8953977	ID=MsG0480018721.01.T01:cds;Parent=MsG0480018721.01.T01
Chr4	CDS	8958590	8958974	8958590	ID=MsG0480018721.01.T01:cds;Parent=MsG0480018721.01.T01
Chr4	CDS	8961541	8962290	8961541	ID=MsG0480018721.01.T01:cds;Parent=MsG0480018721.01.T01
Chr4	CDS	8962910	8963734	8962910	ID=MsG0480018721.01.T01:cds;Parent=MsG0480018721.01.T01

Gene Sequence

>MsG0480018721.01.T01

ATGTTTCTTTCGTTGGCTTTGAGCCTCCTTGGAGCTTCCTTGACATCAGATTTTACCTTGACTCGTGGACTTGAACTCTTTTTGAATGTGCCAAAACAAGAACAAACTTTTACATCACCAAACTATTTAAGTTTGGGTGGAAGAGGGTTGTTGACAGTTATGGTATCAAAGAATAAGTGGGTGATTAAAATTTATGAATTGAAGCAAATGTGGGCAAGTCCATACTTGCGAGACCATTTTGTGTGTGGAGTCGGAACGAAATCAATTTGCGAAGGTGTGAATTCATTTATCAAGAAGTATGTTCAAAACAAAAATAGCCATGTTGATTTGTTACATAATTTTGAAAGAGCTGTCAAAGACTACAAACAGAATGAATTGTTGGCTGATTTCAAATTGTTTTATTATAAACCAGTGTTGACTACTTCCTTGCCTGGAATTGAGCATGGAACTTCAATAATATTCACTTTTATAAAGTTCCAAGAAGTCAAAAAGACGATAGAGGATGTTGCGGGGTTTAGGGAATACAAGAGCAACACATTGTTGAAAAAAGTGGGCATTGAAGAGCCAAAAACAACACGAAGAGTTCTACTTGCTGACATGGCTTCTCAACACGAACAACAACAAGAGTCAAAAACCATTCCCCTTAAAATCATAGTAGACAAACAAAGCAACAAAGTTATTTTTCTGGAAGCAACCAAGGATTTTGTTGACACTCTCTTTAGCTTCCTATCTCTTCCACTTGGCACCATTGTCCGCCTTTTAGCCACCATAAACAGCAACATTGATCAACCAAACAAACCCTCAGAATCATCACCATTCCTTGAAAACATCAAAAACCTCTACCAAACCGTCCAAGACCTACACTCAGACGATGTTTGGAACACAACTTTTGTCAAACAAATGTTGTTGTATCCCAGGAATCCATGTGAATCACTGTGCATGAAGATGTTCTTGAACGTTGATGACACTGAACCATTAAACAAGTTTTTTGTGTGTGATTCTTGTAACAAGTTCACTACTTTTCAAAACCTTTATTGCACTTGTGGACAGCCAACAAATCAACAGCCTCGAAATTTGGATTCGGAGGGTCAAGAGAATAGTAATAGTGCGGAAGCCCAAAATGGACCAAAGTTTCTGGTGTCTGATGATTTGAAGATTGTTCCATGTTCGTCTGTGACTTACTTGCAGCTGTTGATGGAATTGGGGTATTCAGACGCAACTCAATTAGAGGAAGTCACACACAACATTGGAAAACAGGAGATATTGAAACTACTCAAGAATACCTTAACCTCTAATGAGCCTCTCACAAATACAATTTTGGCAAGCAGCTCCAAAAATAAAGACAGCCTACCTAAGCAATCTGCATCAACAGTTAGATTAATGCCTTGCACCGGTAACATAAGTAAAATCAATGTTAAGGTAGTGCAAAGTAAATCTCAGAAAAAGGTGATATATGCAGAAGCCAACGGTGATTTTGTTGACTTCATTTTTAGTTTCCTTACCATGCCATTAGGATCCATTGTAAAGCTTTTGGGATCTAACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTGGAGGACCTTAACCCTGTTTCTGTGTTACTCAATCCTGGTATAGCACCACAGTTTGGTTGTCCAAACCAACCACTCAACATTCCTCAGATTGAACACCCTGCTTATTATTATGGTACAGGAACACCAAAGAGAAAGAGGGATCAACATTCCTCAGATTTCGCGGATTATAATGGTAATCGTATAATGATGGATTGTAGTGGTAATACTGTGATAGAAGAAAAGATAGAAGGAGGAGTGATTTCTAAGTCACGCGAATCAATTTATTTTGCGAAGTCCTTAATTGCACTGGAACCAAGGTCCCCCAACAGGTCAAAAATAGGTGTTGTGGGATTTGTGAAGAGAGAAGGGTTATATTGTGTAGGAGATGATCTAAAAGCAAAACCACTTTCTGTTCATTCGCATCTTTCATTTTTGAAAGAGTTAAGCCTTCCTCTTGATGATCTTGAAATGAAGCTGATAAGCATTGGTGAAGTTGAGGTAAAGATGCTATTATTTTATCACTAA

Protein sequence

>MsG0480018721.01.T01

MFLSLALSLLGASLTSDFTLTRGLELFLNVPKQEQTFTSPNYLSLGGRGLLTVMVSKNKWVIKIYELKQMWASPYLRDHFVCGVGTKSICEGVNSFIKKYVQNKNSHVDLLHNFERAVKDYKQNELLADFKLFYYKPVLTTSLPGIEHGTSIIFTFIKFQEVKKTIEDVAGFREYKSNTLLKKVGIEEPKTTRRVLLADMASQHEQQQESKTIPLKIIVDKQSNKVIFLEATKDFVDTLFSFLSLPLGTIVRLLATINSNIDQPNKPSESSPFLENIKNLYQTVQDLHSDDVWNTTFVKQMLLYPRNPCESLCMKMFLNVDDTEPLNKFFVCDSCNKFTTFQNLYCTCGQPTNQQPRNLDSEGQENSNSAEAQNGPKFLVSDDLKIVPCSSVTYLQLLMELGYSDATQLEEVTHNIGKQEILKLLKNTLTSNEPLTNTILASSSKNKDSLPKQSASTVRLMPCTGNISKINVKVVQSKSQKKVIYAEANGDFVDFIFSFLTMPLGSIVKLLGSNSFAGCVGNLYKSVEDLNPVSVLLNPGIAPQFGCPNQPLNIPQIEHPAYYYGTGTPKRKRDQHSSDFADYNGNRIMMDCSGNTVIEEKIEGGVISKSRESIYFAKSLIALEPRSPNRSKIGVVGFVKREGLYCVGDDLKAKPLSVHSHLSFLKELSLPLDDLEMKLISIGEVEVKMLLFYH*