AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018751.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018751.01.T01 MTR_4g017610 89.666 329 33 1 37 364 1 329 0.0 601
MsG0480018751.01.T01 MTR_4g017410 69.817 328 91 4 43 362 4 331 1.42e-156 450
MsG0480018751.01.T01 MTR_4g017830 61.890 328 122 2 37 363 1 326 2.27e-140 410
MsG0480018751.01.T01 MTR_4g017830 24.756 307 170 9 1 276 221 497 2.05e-12 68.9
MsG0480018751.01.T01 MTR_4g019340 64.134 329 105 5 47 364 37 363 2.34e-140 409
MsG0480018751.01.T01 MTR_4g019340 27.509 269 170 7 1 268 257 501 2.49e-14 74.7
MsG0480018751.01.T01 MTR_4g017440 64.478 335 109 4 37 363 1 333 5.10e-137 400
MsG0480018751.01.T01 MTR_4g017550 66.990 309 92 5 62 364 3 307 6.92e-136 396
MsG0480018751.01.T01 MTR_4g017550 27.016 248 131 9 51 276 250 469 1.25e-11 66.2
MsG0480018751.01.T01 MTR_4g017470 55.856 333 103 4 37 364 1 294 4.74e-114 341
MsG0480018751.01.T01 MTR_4g019310 40.181 331 177 10 44 363 5 325 5.96e-65 214
MsG0480018751.01.T01 MTR_7g066340 37.463 339 181 10 37 362 1 321 4.80e-62 206
MsG0480018751.01.T01 MTR_7g066340 28.814 236 126 8 41 276 256 449 4.20e-15 77.0
MsG0480018751.01.T01 MTR_7g066260 36.842 342 188 9 34 362 1 327 3.22e-61 205
MsG0480018751.01.T01 MTR_7g066350 38.080 323 174 7 51 362 12 319 1.28e-59 199
MsG0480018751.01.T01 MTR_7g058710 36.522 345 166 9 37 362 1 311 2.77e-56 191
MsG0480018751.01.T01 MTR_1g041315 36.390 349 186 12 36 362 2 336 4.69e-55 188
MsG0480018751.01.T01 MTR_7g066570 38.253 332 166 12 51 369 17 322 1.49e-54 189
MsG0480018751.01.T01 MTR_7g066570 28.099 242 145 6 45 276 254 476 1.73e-13 72.0
MsG0480018751.01.T01 MTR_7g012680 35.549 346 161 12 34 362 1 301 1.07e-53 184
MsG0480018751.01.T01 MTR_7g066290 37.538 325 175 9 34 346 1 309 6.30e-52 179
MsG0480018751.01.T01 MTR_7g066430 36.280 328 174 12 51 362 7 315 1.94e-48 171
MsG0480018751.01.T01 MTR_7g066430 25.564 266 170 5 22 276 233 481 1.22e-14 75.5
MsG0480018751.01.T01 MTR_7g063660 36.533 323 172 13 51 364 12 310 8.03e-48 169
MsG0480018751.01.T01 MTR_7g066410 34.111 343 184 11 55 367 17 347 3.83e-47 168
MsG0480018751.01.T01 MTR_7g066410 26.199 271 167 8 21 276 256 508 1.71e-11 65.9
MsG0480018751.01.T01 MTR_7g063880 34.718 337 178 13 34 362 1 303 5.67e-45 161
MsG0480018751.01.T01 MTR_4g019240 62.185 119 43 1 40 158 5 121 1.53e-44 152
MsG0480018751.01.T01 MTR_7g066360 35.423 319 179 10 36 342 2 305 2.63e-44 159
MsG0480018751.01.T01 MTR_7g066400 32.743 339 174 10 37 362 1 298 9.73e-44 157
MsG0480018751.01.T01 MTR_7g066580 33.962 318 154 10 55 362 2 273 2.78e-43 156
MsG0480018751.01.T01 MTR_7g066580 28.163 245 148 7 45 276 212 441 1.51e-11 65.9
MsG0480018751.01.T01 MTR_7g063890 33.529 340 194 9 33 362 9 326 4.54e-43 157
MsG0480018751.01.T01 MTR_7g063820 37.353 340 171 15 37 362 1 312 6.12e-43 156
MsG0480018751.01.T01 MTR_7g063900 35.692 325 173 11 51 364 13 312 1.04e-42 155
MsG0480018751.01.T01 MTR_7g058700 33.828 337 144 12 54 362 525 810 1.32e-42 159
MsG0480018751.01.T01 MTR_7g063830 34.675 323 177 12 51 362 13 312 4.27e-42 153
MsG0480018751.01.T01 MTR_7g062300 33.038 339 192 10 34 362 1 314 7.74e-41 150
MsG0480018751.01.T01 MTR_7g062280 33.333 351 182 13 36 362 2 324 3.13e-40 149
MsG0480018751.01.T01 MTR_7g063840 33.030 330 185 11 43 362 5 308 6.35e-40 147
MsG0480018751.01.T01 MTR_7g066480 35.404 322 179 11 51 362 19 321 1.36e-39 146
MsG0480018751.01.T01 MTR_7g066490 32.416 327 178 10 51 367 19 312 8.00e-37 138
MsG0480018751.01.T01 MTR_2g029400 34.894 235 96 3 44 278 10 187 1.64e-35 134
MsG0480018751.01.T01 MTR_7g066310 31.776 321 131 9 51 362 10 251 2.85e-34 131
MsG0480018751.01.T01 MTR_7g063920 31.532 333 170 10 34 364 1 277 3.74e-34 131
MsG0480018751.01.T01 MTR_7g063670 30.595 353 190 12 37 371 4 319 4.01e-32 126
MsG0480018751.01.T01 MTR_1g014060 35.242 227 124 6 57 276 9 219 9.89e-32 124
MsG0480018751.01.T01 MTR_3g007740 29.846 325 195 7 44 362 5 302 1.50e-30 121
MsG0480018751.01.T01 MTR_3g065200 31.650 297 146 11 51 342 6 250 2.91e-30 120
MsG0480018751.01.T01 MTR_7g062270 36.735 196 94 9 43 228 5 180 2.09e-21 95.1
MsG0480018751.01.T01 MTR_7g066280 31.381 239 122 11 135 362 1 208 3.02e-21 94.7
MsG0480018751.01.T01 MTR_8g023240 25.000 328 201 8 48 363 37 331 3.33e-18 86.7
MsG0480018751.01.T01 MTR_8g445860 27.642 246 145 6 46 278 11 236 1.53e-16 78.6
MsG0480018751.01.T01 MTR_6g006800 75.556 45 11 0 39 83 2 46 2.59e-15 70.5
MsG0480018751.01.T01 MTR_1g115440 27.426 237 150 6 51 278 14 237 6.63e-15 73.9
MsG0480018751.01.T01 MTR_1g115455 28.514 249 128 10 51 278 12 231 2.20e-13 69.3
MsG0480018751.01.T01 MTR_7g066680 36.134 119 64 2 251 362 31 144 1.56e-12 68.6
MsG0480018751.01.T01 MTR_0150s0010 29.167 240 142 8 51 278 13 236 3.68e-12 65.9
MsG0480018751.01.T01 MTR_1g115435 26.582 237 143 6 46 282 5 210 4.23e-12 65.5
MsG0480018751.01.T01 MTR_7g063720 25.641 234 133 8 49 276 24 222 5.28e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018751.01.T01 AT3G09110 33.841 328 193 9 45 364 6 317 6.31e-48 166
MsG0480018751.01.T01 AT3G09140 32.464 345 202 9 42 364 3 338 4.84e-45 162
MsG0480018751.01.T01 AT3G09120 34.337 332 179 13 45 358 3 313 2.02e-39 142
MsG0480018751.01.T01 AT3G09140 30.027 373 202 10 42 364 3 366 2.38e-39 147
MsG0480018751.01.T01 AT5G43240 33.770 305 177 10 51 341 12 305 2.04e-38 144
MsG0480018751.01.T01 AT5G43240 33.770 305 177 10 51 341 12 305 2.04e-38 144
MsG0480018751.01.T01 AT5G43240 33.770 305 177 10 51 341 12 305 2.04e-38 144
MsG0480018751.01.T01 AT5G01120 30.514 331 210 7 45 364 6 327 3.76e-38 143
MsG0480018751.01.T01 AT5G01150 30.347 346 193 10 42 364 3 323 4.39e-38 143
MsG0480018751.01.T01 AT5G01130 31.045 335 194 11 42 364 3 312 5.60e-36 136
MsG0480018751.01.T01 AT5G01140 32.132 333 181 11 42 364 3 300 1.99e-34 132
MsG0480018751.01.T01 AT5G37320 31.722 331 196 13 55 366 18 337 1.73e-31 124

Find 68 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCCTTACCATTCCATT+AGG 0.251119 4:+9389867 MsG0480018751.01.T01:CDS
TAATAGACAACCTAAGAATT+TGG 0.262420 4:+9388920 MsG0480018751.01.T01:CDS
CAATTATAAGATAAATAAAA+TGG 0.268027 4:+9389764 MsG0480018751.01.T01:CDS
CAGGGGAATCATGCCTCTAA+TGG 0.286513 4:+9388954 MsG0480018751.01.T01:CDS
CGTCGTCTGAGTTGAGGTTT+TGG 0.288022 4:-9388716 None:intergenic
GGGTATTAAAGTCTTTATAA+TGG 0.290144 4:-9389892 None:intergenic
CCCTCAGAGTCCAAATTCTT+AGG 0.309465 4:-9388930 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.310647 4:-9388918 None:intergenic
ATTCTTCTCCATCAACATAT+TGG 0.310876 4:-9389737 None:intergenic
TCAGAATCATCACCATTCTT+TGG 0.319703 4:+9388666 MsG0480018751.01.T01:CDS
ATGCAGATGTTGCTGGAATC+AGG 0.320991 4:+9389056 MsG0480018751.01.T01:CDS
TCCAATATTGTATTCGTAAG+AGG 0.327753 4:-9389681 None:intergenic
AGCAACATTTGCTTACATAA+AGG 0.329982 4:-9388750 None:intergenic
TGTTGTTGGTGGTTGCTAAA+AGG 0.334739 4:-9388626 None:intergenic
GCCTCTTACGAATACAATAT+TGG 0.339705 4:+9389680 MsG0480018751.01.T01:CDS
GTTTCCTTACCATTCCATTA+GGG 0.340279 4:+9389868 MsG0480018751.01.T01:CDS
GCAACATTTGCTTACATAAA+GGG 0.341137 4:-9388749 None:intergenic
TGTATCAAACACCCCATTAG+AGG 0.344943 4:-9388967 None:intergenic
ACCTCAACTCAGACGACGTT+TGG 0.355641 4:+9388721 MsG0480018751.01.T01:CDS
ATATGTTGATGGAGAAGAAT+TGG 0.357603 4:+9389740 MsG0480018751.01.T01:CDS
CAGACATAAAACTTACTTGA+TGG 0.362318 4:-9388837 None:intergenic
AGGGGAATCATGCCTCTAAT+GGG 0.380156 4:+9388955 MsG0480018751.01.T01:CDS
GAACTCAATGCAGATGTTGC+TGG 0.386915 4:+9389049 MsG0480018751.01.T01:CDS
GAATTTGGACTCTGAGGGTC+AGG 0.397162 4:+9388935 MsG0480018751.01.T01:CDS
GTCTTTATAATGGACCCTAA+TGG 0.418519 4:-9389882 None:intergenic
GCATAAGTGTATTGTTAGAA+AGG 0.437351 4:-9387091 None:intergenic
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4:+9388591 MsG0480018751.01.T01:CDS
AAGAGGCTCATGAGAGGTTA+AGG 0.445820 4:-9389664 None:intergenic
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4:-9388596 None:intergenic
GAGAGGTTAAGGTATACTTC+AGG 0.468779 4:-9389653 None:intergenic
TATGTTGATGGAGAAGAATT+GGG 0.477595 4:+9389741 MsG0480018751.01.T01:CDS
CCACGGAACCAATATGTTGA+TGG 0.488534 4:+9389729 MsG0480018751.01.T01:CDS
TTGTCGATCATTGTTGTTGG+TGG 0.499724 4:-9388637 None:intergenic
ATTGAGTTCAGCAAGGAACT+TGG 0.501314 4:-9389035 None:intergenic
AACACATCCAGGAAAATTGT+TGG 0.509207 4:-9389913 None:intergenic
CACACAAAGCATTGGCAAGA+AGG 0.511399 4:+9389109 MsG0480018751.01.T01:CDS
CGAAACCTTGACTGCACCTG+CGG 0.512942 4:+9388888 MsG0480018751.01.T01:CDS
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4:-9388915 None:intergenic
GTTAGAAAGGTTGTGATGGT+TGG 0.531870 4:-9387078 None:intergenic
CCATCAACATATTGGTTCCG+TGG 0.536459 4:-9389729 None:intergenic
TCCAAACGTCGTCTGAGTTG+AGG 0.536465 4:-9388722 None:intergenic
TGCAGATGTTGCTGGAATCA+GGG 0.536790 4:+9389057 MsG0480018751.01.T01:CDS
TGTTTGATGTTGCCAAAGAA+TGG 0.539653 4:-9388678 None:intergenic
TACAAATTACCAACACATCC+AGG 0.541284 4:-9389924 None:intergenic
ACACATCCAGGAAAATTGTT+GGG 0.541913 4:-9389912 None:intergenic
ATCATCATTGCAGAAGCTGA+TGG 0.549559 4:+9389822 MsG0480018751.01.T01:CDS
ATTCATGTGAATCACTATGC+AGG 0.552422 4:+9388784 MsG0480018751.01.T01:CDS
TTGTTGTTGTTGAGAAGTCA+TGG 0.559710 4:-9388469 None:intergenic
ACCTAAGAATTTGGACTCTG+AGG 0.575501 4:+9388929 MsG0480018751.01.T01:CDS
AGTGATTCACATGAATTCCT+CGG 0.577391 4:-9388777 None:intergenic
TGGACCCTAATGGAATGGTA+AGG 0.578424 4:-9389872 None:intergenic
TATAATGGACCCTAATGGAA+TGG 0.581198 4:-9389877 None:intergenic
AGCAAATGTTGCTGCATCCG+AGG 0.591888 4:+9388760 MsG0480018751.01.T01:CDS
CCTAAGAATTTGGACTCTGA+GGG 0.592717 4:+9388930 MsG0480018751.01.T01:CDS
AATTTGGACTCTGAGGGTCA+GGG 0.595588 4:+9388936 MsG0480018751.01.T01:CDS
ATTTGGACTCTGAGGGTCAG+GGG 0.598326 4:+9388937 MsG0480018751.01.T01:CDS
GAGGAAGTCACACAAAGCAT+TGG 0.599245 4:+9389101 MsG0480018751.01.T01:CDS
TATTGTTAGAAAGGTTGTGA+TGG 0.617762 4:-9387082 None:intergenic
AGGTTAAGGTATACTTCAGG+AGG 0.621580 4:-9389650 None:intergenic
TTCAGACTCAACGCAACTAG+AGG 0.625063 4:+9389082 MsG0480018751.01.T01:CDS
AACGTTTCCCCTCAAAATCA+CGG 0.627379 4:+9388503 MsG0480018751.01.T01:CDS
GGGGAATCATGCCTCTAATG+GGG 0.639572 4:+9388956 MsG0480018751.01.T01:CDS
CAAAGAACAATGACGAACCA+CGG 0.642115 4:+9389712 MsG0480018751.01.T01:CDS
ACAAAGCATTGGCAAGAAGG+AGG 0.647102 4:+9389112 MsG0480018751.01.T01:CDS
GGTTGCTAAAAGGCGAACGA+TGG 0.680679 4:-9388616 None:intergenic
ATTCGTAAGAGGCTCATGAG+AGG 0.692406 4:-9389670 None:intergenic
CATCTGCATTGAGTTCAGCA+AGG 0.698278 4:-9389042 None:intergenic
AGGCGAACGATGGTACCAAG+TGG 0.729828 4:-9388606 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATAAGATAAATAAAA+TGG + Chr4:9389764-9389783 MsG0480018751.01.T01:CDS 10.0%
!! TAATAATAATATGTATTTGT+TGG + Chr4:9387782-9387801 MsG0480018751.01.T01:intron 10.0%
!!! TTCATTTTTATTATATTTAG+AGG + Chr4:9387239-9387258 MsG0480018751.01.T01:intron 10.0%
!! ATAAAATGTTAAAACACAAA+TGG - Chr4:9389618-9389637 None:intergenic 15.0%
!! ATATTATTGTTGAAAATTTC+TGG - Chr4:9388264-9388283 None:intergenic 15.0%
!! TAAATATGGTCTTTATAATT+TGG + Chr4:9387433-9387452 MsG0480018751.01.T01:intron 15.0%
!! TATTATTGTTGAAAATTTCT+GGG - Chr4:9388263-9388282 None:intergenic 15.0%
!! TTTCATCTTAAATAATGATT+TGG - Chr4:9387939-9387958 None:intergenic 15.0%
!!! AAATTTAAAATCATAGGTTA+AGG - Chr4:9389256-9389275 None:intergenic 15.0%
!!! ATTTTCTTTTCTTTTTTAGA+TGG + Chr4:9387750-9387769 MsG0480018751.01.T01:intron 15.0%
!!! TAACCTATGATTTTAAATTT+AGG + Chr4:9389256-9389275 MsG0480018751.01.T01:intron 15.0%
!!! TAATTTTGTTTCCATTATTT+TGG - Chr4:9387851-9387870 None:intergenic 15.0%
!!! TTTTAACATAATCTATGTTT+CGG - Chr4:9387907-9387926 None:intergenic 15.0%
!!! TTTTCTTGAAAATATAATCA+GGG - Chr4:9387877-9387896 None:intergenic 15.0%
!! AAAACTACAAATATACATGT+TGG - Chr4:9387480-9387499 None:intergenic 20.0%
!! AATAAAGAGAAATAGAACTT+TGG - Chr4:9389549-9389568 None:intergenic 20.0%
!! ACTTAATCAAAAGTAATCAA+AGG + Chr4:9387185-9387204 MsG0480018751.01.T01:intron 20.0%
!! GATATAAATATGTATGCATA+AGG - Chr4:9388299-9388318 None:intergenic 20.0%
!! GGATATATATAGTTAATACT+TGG + Chr4:9389398-9389417 MsG0480018751.01.T01:intron 20.0%
!! TTTGTACATTAATGAAAGTT+GGG - Chr4:9387519-9387538 None:intergenic 20.0%
!!! CTTTTCTTGAAAATATAATC+AGG - Chr4:9387878-9387897 None:intergenic 20.0%
!!! GATTTTATTGACTTCATTTT+TGG + Chr4:9389846-9389865 MsG0480018751.01.T01:CDS 20.0%
!!! TGATTACTTTTGATTAAGTA+TGG - Chr4:9387184-9387203 None:intergenic 20.0%
! AAGAACACTACTAGAAAAAT+CGG + Chr4:9389957-9389976 MsG0480018751.01.T01:CDS 25.0%
! AAGGCTTAATCAAAAGTAAT+AGG + Chr4:9387204-9387223 MsG0480018751.01.T01:intron 25.0%
! ACCATATTTAGCTAAATGAA+TGG - Chr4:9387423-9387442 None:intergenic 25.0%
! CAACTTTCATTAATGTACAA+AGG + Chr4:9387518-9387537 MsG0480018751.01.T01:intron 25.0%
! CTTTGTACATTAATGAAAGT+TGG - Chr4:9387520-9387539 None:intergenic 25.0%
! GAAACATAACACCAAAATAA+TGG + Chr4:9387837-9387856 MsG0480018751.01.T01:intron 25.0%
! GGGTATTAAAGTCTTTATAA+TGG - Chr4:9389895-9389914 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG + Chr4:9388920-9388939 MsG0480018751.01.T01:CDS 25.0%
! TCTCATTGTTGAAAAAAAGT+AGG + Chr4:9388405-9388424 MsG0480018751.01.T01:intron 25.0%
! TGAATCTTATGCTTCTAAAT+TGG + Chr4:9387280-9387299 MsG0480018751.01.T01:intron 25.0%
! TTTCGTTGTGAATCTATATT+GGG + Chr4:9389284-9389303 MsG0480018751.01.T01:intron 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG - Chr4:9388921-9388940 None:intergenic 25.0%
!! GCACCTAAATTTAAAATCAT+AGG - Chr4:9389262-9389281 None:intergenic 25.0%
!! TATCTAAAACCATTACATTG+TGG + Chr4:9389354-9389373 MsG0480018751.01.T01:intron 25.0%
AAAAGAAAAGAAAATGCACG+AGG - Chr4:9387745-9387764 None:intergenic 30.0%
AAATCTTCTTCGTTCTTCTT+CGG + Chr4:9387686-9387705 MsG0480018751.01.T01:intron 30.0%
AAGAGAAATAGAACTTTGGT+AGG - Chr4:9389545-9389564 None:intergenic 30.0%
AATGCAAAAGATAGTAGCAA+TGG + Chr4:9387957-9387976 MsG0480018751.01.T01:intron 30.0%
AATGGAATAGGAACAAACAA+TGG - Chr4:9388321-9388340 None:intergenic 30.0%
AGAGAAATAGAACTTTGGTA+GGG - Chr4:9389544-9389563 None:intergenic 30.0%
AGCAACATTTGCTTACATAA+AGG - Chr4:9388753-9388772 None:intergenic 30.0%
AGCATAAGATTCACAGTTAA+AGG - Chr4:9387273-9387292 None:intergenic 30.0%
AGTATGATGTTATCTGTTAG+TGG + Chr4:9389430-9389449 MsG0480018751.01.T01:intron 30.0%
ATCTATATTGGGGTAAAATG+TGG + Chr4:9389295-9389314 MsG0480018751.01.T01:intron 30.0%
ATGAAGCTAAGAAAAGGATT+TGG - Chr4:9387715-9387734 None:intergenic 30.0%
ATTCTTCTCCATCAACATAT+TGG - Chr4:9389740-9389759 None:intergenic 30.0%
CAAATGGTTAGAATGAAAGT+TGG - Chr4:9389602-9389621 None:intergenic 30.0%
CAGACATAAAACTTACTTGA+TGG - Chr4:9388840-9388859 None:intergenic 30.0%
CTATGTCAGATCTATTATTG+TGG + Chr4:9387326-9387345 MsG0480018751.01.T01:intron 30.0%
CTGCAATGACAATATTTGTT+AGG + Chr4:9389377-9389396 MsG0480018751.01.T01:intron 30.0%
GCAACATTTGCTTACATAAA+GGG - Chr4:9388752-9388771 None:intergenic 30.0%
GCATAAGTGTATTGTTAGAA+AGG - Chr4:9387094-9387113 None:intergenic 30.0%
GCCATTCATTTAGCTAAATA+TGG + Chr4:9387419-9387438 MsG0480018751.01.T01:intron 30.0%
GTAACATGATGATTGCAATT+AGG + Chr4:9387979-9387998 MsG0480018751.01.T01:intron 30.0%
GTTTCGTTGTGAATCTATAT+TGG + Chr4:9389283-9389302 MsG0480018751.01.T01:intron 30.0%
GTTTGTTCCTATTCCATTTA+GGG + Chr4:9388323-9388342 MsG0480018751.01.T01:intron 30.0%
TATTGTTAGAAAGGTTGTGA+TGG - Chr4:9387085-9387104 None:intergenic 30.0%
TATTTACTCAGTTGGAGTTT+GGG + Chr4:9387647-9387666 MsG0480018751.01.T01:intron 30.0%
TCCAATATTGTATTCGTAAG+AGG - Chr4:9389684-9389703 None:intergenic 30.0%
TGGAAAAAAACTCAATGGAA+CGG - Chr4:9389197-9389216 None:intergenic 30.0%
TGTTTGTTCCTATTCCATTT+AGG + Chr4:9388322-9388341 MsG0480018751.01.T01:intron 30.0%
TTAATGTACAAAGGACGATT+TGG + Chr4:9387527-9387546 MsG0480018751.01.T01:intron 30.0%
TTCGTTGTGAATCTATATTG+GGG + Chr4:9389285-9389304 MsG0480018751.01.T01:intron 30.0%
TTTGTTCCTATTCCATTTAG+GGG + Chr4:9388324-9388343 MsG0480018751.01.T01:intron 30.0%
! AACAACTTTTCCTCAGTAAA+AGG + Chr4:9387596-9387615 MsG0480018751.01.T01:intron 30.0%
! ACAACTTTTCCTCAGTAAAA+GGG + Chr4:9387597-9387616 MsG0480018751.01.T01:intron 30.0%
! ATATGTTGATGGAGAAGAAT+TGG + Chr4:9389740-9389759 MsG0480018751.01.T01:CDS 30.0%
! TATGTTGATGGAGAAGAATT+GGG + Chr4:9389741-9389760 MsG0480018751.01.T01:CDS 30.0%
! TTAATACCCAACAATTTTCC+TGG + Chr4:9389906-9389925 MsG0480018751.01.T01:CDS 30.0%
! TTTTTGTCGATCATTGTTGT+TGG - Chr4:9388643-9388662 None:intergenic 30.0%
!!! AAATCTGTCATTTTAAGCCA+CGG - Chr4:9388157-9388176 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG + Chr4:9388536-9388555 MsG0480018751.01.T01:CDS 30.0%
!!! ACTGAGGAAAAGTTGTTTTT+TGG - Chr4:9387593-9387612 None:intergenic 30.0%
!!! CTGAGGAAAAGTTGTTTTTT+GGG - Chr4:9387592-9387611 None:intergenic 30.0%
!!! GTTTGTTGTGTTTCCATTTT+AGG + Chr4:9389160-9389179 MsG0480018751.01.T01:intron 30.0%
!!! TGTCTTATTTTCTACTTCCT+CGG - Chr4:9387625-9387644 None:intergenic 30.0%
!!! TGTTTTTTGGGTACATAATC+AGG - Chr4:9387580-9387599 None:intergenic 30.0%
AACACATCCAGGAAAATTGT+TGG - Chr4:9389916-9389935 None:intergenic 35.0%
AAGACACGTATTTACTCAGT+TGG + Chr4:9387639-9387658 MsG0480018751.01.T01:intron 35.0%
AATCACCAAAGTCACTTAAG+TGG - Chr4:9388372-9388391 None:intergenic 35.0%
ACACATCCAGGAAAATTGTT+GGG - Chr4:9389915-9389934 None:intergenic 35.0%
AGTATGTGATCTAAACTGAG+AGG + Chr4:9387116-9387135 MsG0480018751.01.T01:intron 35.0%
AGTGATTCACATGAATTCCT+CGG - Chr4:9388780-9388799 None:intergenic 35.0%
ATGTTATCTGTTAGTGGTAG+AGG + Chr4:9389436-9389455 MsG0480018751.01.T01:intron 35.0%
ATTCATGTGAATCACTATGC+AGG + Chr4:9388784-9388803 MsG0480018751.01.T01:CDS 35.0%
CTATTCCATTTAGGGGTTTA+GGG + Chr4:9388331-9388350 MsG0480018751.01.T01:intron 35.0%
GATTAGATCATGTGGTGTTT+AGG + Chr4:9388030-9388049 MsG0480018751.01.T01:intron 35.0%
GCAAGTGGAAAAAAACTCAA+TGG - Chr4:9389202-9389221 None:intergenic 35.0%
GCCTCTTACGAATACAATAT+TGG + Chr4:9389680-9389699 MsG0480018751.01.T01:CDS 35.0%
GGAAAAAAACTCAATGGAAC+GGG - Chr4:9389196-9389215 None:intergenic 35.0%
GTATGTGATCTAAACTGAGA+GGG + Chr4:9387117-9387136 MsG0480018751.01.T01:intron 35.0%
GTATTTACTCAGTTGGAGTT+TGG + Chr4:9387646-9387665 MsG0480018751.01.T01:intron 35.0%
GTCTATAACATATTGACACG+CGG + Chr4:9388088-9388107 MsG0480018751.01.T01:intron 35.0%
GTTTCCTTACCATTCCATTA+GGG + Chr4:9389868-9389887 MsG0480018751.01.T01:CDS 35.0%
TAAATGAATGGCATCTTGGT+TGG - Chr4:9387411-9387430 None:intergenic 35.0%
TACAAATTACCAACACATCC+AGG - Chr4:9389927-9389946 None:intergenic 35.0%
TACCTGATGAAGCTAAGAAA+AGG - Chr4:9387721-9387740 None:intergenic 35.0%
TAGCTAAATGAATGGCATCT+TGG - Chr4:9387415-9387434 None:intergenic 35.0%
TCAGAATCATCACCATTCTT+TGG + Chr4:9388666-9388685 MsG0480018751.01.T01:CDS 35.0%
TGTGGTGTTTAGGAAATGTT+TGG + Chr4:9388040-9388059 MsG0480018751.01.T01:intron 35.0%
TGTTTGATGTTGCCAAAGAA+TGG - Chr4:9388681-9388700 None:intergenic 35.0%
TTTGGGTACATAATCAGGAA+AGG - Chr4:9387575-9387594 None:intergenic 35.0%
! AACAATTTTCCTGGATGTGT+TGG + Chr4:9389915-9389934 MsG0480018751.01.T01:CDS 35.0%
! AGAAAAATCGGATTTTGCGA+CGG + Chr4:9389969-9389988 MsG0480018751.01.T01:CDS 35.0%
! ATCCTTTTCTTAGCTTCATC+AGG + Chr4:9387716-9387735 MsG0480018751.01.T01:intron 35.0%
! CAACTTTTCCTCAGTAAAAG+GGG + Chr4:9387598-9387617 MsG0480018751.01.T01:intron 35.0%
! GGGTGTTGTACAAAAACTAA+TGG + Chr4:9387137-9387156 MsG0480018751.01.T01:intron 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG - Chr4:9388918-9388937 None:intergenic 35.0%
! TTGTTGTTGTTGAGAAGTCA+TGG - Chr4:9388472-9388491 None:intergenic 35.0%
! TTTGTCTACCGTGATTTTGA+GGG - Chr4:9388514-9388533 None:intergenic 35.0%
!! AGGAAATGTTTGGTGTATTG+AGG + Chr4:9388050-9388069 MsG0480018751.01.T01:intron 35.0%
!! CTTGTTTCTACAATGACCTA+AGG - Chr4:9389231-9389250 None:intergenic 35.0%
!! GGAAATGTTTGGTGTATTGA+GGG + Chr4:9388051-9388070 MsG0480018751.01.T01:intron 35.0%
!! GTCTTTATAATGGACCCTAA+TGG - Chr4:9389885-9389904 None:intergenic 35.0%
!! TATAATGGACCCTAATGGAA+TGG - Chr4:9389880-9389899 None:intergenic 35.0%
!!! ACACACTTTTTCTTGTGTTG+TGG + Chr4:9387815-9387834 MsG0480018751.01.T01:intron 35.0%
AACGTTTCCCCTCAAAATCA+CGG + Chr4:9388503-9388522 MsG0480018751.01.T01:CDS 40.0%
AATCGATAACACGAAGTAGC+GGG + Chr4:9388206-9388225 MsG0480018751.01.T01:intron 40.0%
ACCTAAGAATTTGGACTCTG+AGG + Chr4:9388929-9388948 MsG0480018751.01.T01:CDS 40.0%
AGGTTAAGGTATACTTCAGG+AGG - Chr4:9389653-9389672 None:intergenic 40.0%
AGTTAGAACTTAGAGGAGAC+TGG + Chr4:9389479-9389498 MsG0480018751.01.T01:intron 40.0%
ATCATCATTGCAGAAGCTGA+TGG + Chr4:9389822-9389841 MsG0480018751.01.T01:CDS 40.0%
ATTGAGTTCAGCAAGGAACT+TGG - Chr4:9389038-9389057 None:intergenic 40.0%
CAAAGAACAATGACGAACCA+CGG + Chr4:9389712-9389731 MsG0480018751.01.T01:CDS 40.0%
CAATCGATAACACGAAGTAG+CGG + Chr4:9388205-9388224 MsG0480018751.01.T01:intron 40.0%
CCTAAACCCCTAAATGGAAT+AGG - Chr4:9388333-9388352 None:intergenic 40.0%
CCTAAGAATTTGGACTCTGA+GGG + Chr4:9388930-9388949 MsG0480018751.01.T01:CDS 40.0%
CCTATTCCATTTAGGGGTTT+AGG + Chr4:9388330-9388349 MsG0480018751.01.T01:intron 40.0%
CGGTTGTAAATATCGGTCAT+GGG + Chr4:9388108-9388127 MsG0480018751.01.T01:intron 40.0%
GAAATAGAACTTTGGTAGGG+TGG - Chr4:9389541-9389560 None:intergenic 40.0%
GAGAGGTTAAGGTATACTTC+AGG - Chr4:9389656-9389675 None:intergenic 40.0%
GGCTGTTAGATTAGATCATG+TGG + Chr4:9388022-9388041 MsG0480018751.01.T01:intron 40.0%
GGGAACTAGTTAGAACTTAG+AGG + Chr4:9389472-9389491 MsG0480018751.01.T01:intron 40.0%
GGTTTCCACTTAAGTGACTT+TGG + Chr4:9388364-9388383 MsG0480018751.01.T01:intron 40.0%
GGTTTCCTTACCATTCCATT+AGG + Chr4:9389867-9389886 MsG0480018751.01.T01:CDS 40.0%
GTTAGAAAGGTTGTGATGGT+TGG - Chr4:9387081-9387100 None:intergenic 40.0%
TACCTCGACAACGACATTAA+TGG - Chr4:9389336-9389355 None:intergenic 40.0%
TCATTGCAGCCACAATGTAA+TGG - Chr4:9389366-9389385 None:intergenic 40.0%
TGACACGCGGTTGTAAATAT+CGG + Chr4:9388101-9388120 MsG0480018751.01.T01:intron 40.0%
TGTATCAAACACCCCATTAG+AGG - Chr4:9388970-9388989 None:intergenic 40.0%
TTCCACTTGCAAATGACCTT+AGG + Chr4:9389212-9389231 MsG0480018751.01.T01:intron 40.0%
TTGCAATTAGGTGACCATGA+TGG + Chr4:9387991-9388010 MsG0480018751.01.T01:intron 40.0%
! GTTTGTCTACCGTGATTTTG+AGG - Chr4:9388515-9388534 None:intergenic 40.0%
! TAAATATCGGTCATGGGCTA+GGG + Chr4:9388114-9388133 MsG0480018751.01.T01:intron 40.0%
! TTGTCGATCATTGTTGTTGG+TGG - Chr4:9388640-9388659 None:intergenic 40.0%
! TTGTCTACCGTGATTTTGAG+GGG - Chr4:9388513-9388532 None:intergenic 40.0%
!! GTAGAGGTGTCATTAGGAAA+GGG + Chr4:9389452-9389471 MsG0480018751.01.T01:intron 40.0%
!! TGGTGTATTGAGGGTTTTGA+AGG + Chr4:9388060-9388079 MsG0480018751.01.T01:intron 40.0%
!! TGTTGTTGGTGGTTGCTAAA+AGG - Chr4:9388629-9388648 None:intergenic 40.0%
!! TTAGTGGTAGAGGTGTCATT+AGG + Chr4:9389446-9389465 MsG0480018751.01.T01:intron 40.0%
!!! AGTTGAGGTTTTGGACAGTT+TGG - Chr4:9388710-9388729 None:intergenic 40.0%
!!! GGTTTTGGACAGTTTGGTAA+AGG - Chr4:9388704-9388723 None:intergenic 40.0%
AAGAGGCTCATGAGAGGTTA+AGG - Chr4:9389667-9389686 None:intergenic 45.0%
ACAGCCATCTCTAACCATCA+TGG - Chr4:9388008-9388027 None:intergenic 45.0%
ACTTTGGTAGGGTGGAACAA+TGG - Chr4:9389533-9389552 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG + Chr4:9388591-9388610 MsG0480018751.01.T01:CDS 45.0%
AGGGGAATCATGCCTCTAAT+GGG + Chr4:9388955-9388974 MsG0480018751.01.T01:CDS 45.0%
ATGCAGATGTTGCTGGAATC+AGG + Chr4:9389056-9389075 MsG0480018751.01.T01:CDS 45.0%
ATTCGTAAGAGGCTCATGAG+AGG - Chr4:9389673-9389692 None:intergenic 45.0%
CAATGCCCTAAACCCCTAAA+TGG - Chr4:9388339-9388358 None:intergenic 45.0%
CATCTGCATTGAGTTCAGCA+AGG - Chr4:9389045-9389064 None:intergenic 45.0%
CCACGGAACCAATATGTTGA+TGG + Chr4:9389729-9389748 MsG0480018751.01.T01:CDS 45.0%
CCCTCAGAGTCCAAATTCTT+AGG - Chr4:9388933-9388952 None:intergenic 45.0%
GAACTCAATGCAGATGTTGC+TGG + Chr4:9389049-9389068 MsG0480018751.01.T01:CDS 45.0%
GACCTAAGGTCATTTGCAAG+TGG - Chr4:9389217-9389236 None:intergenic 45.0%
GAGGAAGTCACACAAAGCAT+TGG + Chr4:9389101-9389120 MsG0480018751.01.T01:CDS 45.0%
GCGGTTGTAAATATCGGTCA+TGG + Chr4:9388107-9388126 MsG0480018751.01.T01:intron 45.0%
GGGTGCATAATGGCCTAAAA+TGG - Chr4:9389176-9389195 None:intergenic 45.0%
GTAAATATCGGTCATGGGCT+AGG + Chr4:9388113-9388132 MsG0480018751.01.T01:intron 45.0%
GTGACCATGATGGTTAGAGA+TGG + Chr4:9388001-9388020 MsG0480018751.01.T01:intron 45.0%
TCAATGGAACGGGTGCATAA+TGG - Chr4:9389186-9389205 None:intergenic 45.0%
TGCAGATGTTGCTGGAATCA+GGG + Chr4:9389057-9389076 MsG0480018751.01.T01:CDS 45.0%
TGCCATTAATGTCGTTGTCG+AGG + Chr4:9389331-9389350 MsG0480018751.01.T01:intron 45.0%
TGGACCCTAATGGAATGGTA+AGG - Chr4:9389875-9389894 None:intergenic 45.0%
TGGCAAGAAGGAGGTAACTA+AGG + Chr4:9389121-9389140 MsG0480018751.01.T01:intron 45.0%
TGGTACCAAGTGGCAAAGAT+AGG - Chr4:9388599-9388618 None:intergenic 45.0%
TTCAGACTCAACGCAACTAG+AGG + Chr4:9389082-9389101 MsG0480018751.01.T01:CDS 45.0%
! AATTTGGACTCTGAGGGTCA+GGG + Chr4:9388936-9388955 MsG0480018751.01.T01:CDS 45.0%
! TCTATTAGGAGGTTTTCCGC+AGG - Chr4:9388907-9388926 None:intergenic 45.0%
!! ACAAAGCATTGGCAAGAAGG+AGG + Chr4:9389112-9389131 MsG0480018751.01.T01:CDS 45.0%
!! CACACAAAGCATTGGCAAGA+AGG + Chr4:9389109-9389128 MsG0480018751.01.T01:CDS 45.0%
!! CCATCAACATATTGGTTCCG+TGG - Chr4:9389732-9389751 None:intergenic 45.0%
!! GGTAGAGGTGTCATTAGGAA+AGG + Chr4:9389451-9389470 MsG0480018751.01.T01:intron 45.0%
ACCTCAACTCAGACGACGTT+TGG + Chr4:9388721-9388740 MsG0480018751.01.T01:CDS 50.0%
AGCAAATGTTGCTGCATCCG+AGG + Chr4:9388760-9388779 MsG0480018751.01.T01:CDS 50.0%
CAGGGGAATCATGCCTCTAA+TGG + Chr4:9388954-9388973 MsG0480018751.01.T01:CDS 50.0%
GGGGAATCATGCCTCTAATG+GGG + Chr4:9388956-9388975 MsG0480018751.01.T01:CDS 50.0%
GGTTGCTAAAAGGCGAACGA+TGG - Chr4:9388619-9388638 None:intergenic 50.0%
TCCAAACGTCGTCTGAGTTG+AGG - Chr4:9388725-9388744 None:intergenic 50.0%
! ATTTGGACTCTGAGGGTCAG+GGG + Chr4:9388937-9388956 MsG0480018751.01.T01:CDS 50.0%
! GAATTTGGACTCTGAGGGTC+AGG + Chr4:9388935-9388954 MsG0480018751.01.T01:CDS 50.0%
! GTGTTATCGATTGCAGACGC+TGG - Chr4:9388198-9388217 None:intergenic 50.0%
!! CGTCGTCTGAGTTGAGGTTT+TGG - Chr4:9388719-9388738 None:intergenic 50.0%
!! GGGGTTTAGGGCATTGAACA+AGG + Chr4:9388343-9388362 MsG0480018751.01.T01:intron 50.0%
!!! CTAGGGCGGATTTTTGGCAA+CGG + Chr4:9388131-9388150 MsG0480018751.01.T01:intron 50.0%
AGGCGAACGATGGTACCAAG+TGG - Chr4:9388609-9388628 None:intergenic 55.0%
CGAAACCTTGACTGCACCTG+CGG + Chr4:9388888-9388907 MsG0480018751.01.T01:CDS 55.0%
CGATTGCAGACGCTGGACTA+TGG - Chr4:9388191-9388210 None:intergenic 55.0%
TCCTCAGTAAAAGGGGACCG+AGG + Chr4:9387605-9387624 MsG0480018751.01.T01:intron 55.0%
! GTTTTCCGCAGGTGCAGTCA+AGG - Chr4:9388896-9388915 None:intergenic 55.0%
! TCCTCGGTCCCCTTTTACTG+AGG - Chr4:9387609-9387628 None:intergenic 55.0%
!! ATATCGGTCATGGGCTAGGG+CGG + Chr4:9388117-9388136 MsG0480018751.01.T01:intron 55.0%
!!! CATGGGCTAGGGCGGATTTT+TGG + Chr4:9388125-9388144 MsG0480018751.01.T01:intron 55.0%
!!! CGGATTTTTGGCAACGGCCG+TGG + Chr4:9388137-9388156 MsG0480018751.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 9387053 9390001 9387053 ID=MsG0480018751.01;Name=MsG0480018751.01
Chr4 mRNA 9387053 9390001 9387053 ID=MsG0480018751.01.T01;Parent=MsG0480018751.01;Name=MsG0480018751.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.66|0.5|0.33|3|0|376
Chr4 exon 9387053 9387116 9387053 ID=MsG0480018751.01.T01:exon:2604;Parent=MsG0480018751.01.T01
Chr4 exon 9388427 9389133 9388427 ID=MsG0480018751.01.T01:exon:2605;Parent=MsG0480018751.01.T01
Chr4 exon 9389642 9390001 9389642 ID=MsG0480018751.01.T01:exon:2606;Parent=MsG0480018751.01.T01
Chr4 CDS 9387053 9387116 9387053 ID=MsG0480018751.01.T01:cds;Parent=MsG0480018751.01.T01
Chr4 CDS 9388427 9389133 9388427 ID=MsG0480018751.01.T01:cds;Parent=MsG0480018751.01.T01
Chr4 CDS 9389642 9390001 9389642 ID=MsG0480018751.01.T01:cds;Parent=MsG0480018751.01.T01
Gene Sequence

>MsG0480018751.01.T01

ATGAATTTTGAACACAAAACTTACTCCAACCATCACAACCTTTCTAACAATACACTTATGCTAAGCATTGAAGAGCTAAAAACAACAAGAGTTCTACAACTTGCTGCCATGACTTCTCAACAACAACAACAAGAGTCGAAAACGTTTCCCCTCAAAATCACGGTAGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGAGACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCAACCACCAACAACAATGATCGACAAAAACTCTCAGAATCATCACCATTCTTTGGCAACATCAAACACCTTTACCAAACTGTCCAAAACCTCAACTCAGACGACGTTTGGAACAACCCTTTATGTAAGCAAATGTTGCTGCATCCGAGGAATTCATGTGAATCACTATGCAGGAAGTTGTACTTGAATATTGATGATTCTGAACCATCAAGTAAGTTTTATGTCTGTGATTCTTGTAACAAGTTCACTACTTTTCGAAACCTTGACTGCACCTGCGGAAAACCTCCTAATAGACAACCTAAGAATTTGGACTCTGAGGGTCAGGGGAATCATGCCTCTAATGGGGTGTTTGATACAGAAAGTGAACAAATGTTTCTAGTGTCTGATGATTTGAATATTGTGCCAAGTTCCTTGCTGAACTCAATGCAGATGTTGCTGGAATCAGGGATTTCAGACTCAACGCAACTAGAGGAAGTCACACAAAGCATTGGCAAGAAGGAGATATTGAACCTCCTGAAGTATACCTTAACCTCTCATGAGCCTCTTACGAATACAATATTGGAAAGTAGCTCAAAGAACAATGACGAACCACGGAACCAATATGTTGATGGAGAAGAATTGGGCAATTATAAGATAAATAAAATGGATATTAAAGTATTGCAAAGCAAATCTCAGAATAAGATCATCATTGCAGAAGCTGATGGAGATTTTATTGACTTCATTTTTGGTTTCCTTACCATTCCATTAGGGTCCATTATAAAGACTTTAATACCCAACAATTTTCCTGGATGTGTTGGTAATTTGTACAACAGTGTGAAGAACACTACTAGAAAAATCGGATTTTGCGACGGAAATTTGTGA
Protein sequence

>MsG0480018751.01.T01

MNFEHKTYSNHHNLSNNTLMLSIEELKTTRVLQLAAMTSQQQQQESKTFPLKITVDKQSNKVVFVEATKDFVETLFSFLSLPLGTIVRLLATTNNNDRQKLSESSPFFGNIKHLYQTVQNLNSDDVWNNPLCKQMLLHPRNSCESLCRKLYLNIDDSEPSSKFYVCDSCNKFTTFRNLDCTCGKPPNRQPKNLDSEGQGNHASNGVFDTESEQMFLVSDDLNIVPSSLLNSMQMLLESGISDSTQLEEVTQSIGKKEILNLLKYTLTSHEPLTNTILESSSKNNDEPRNQYVDGEELGNYKINKMDIKVLQSKSQNKIIIAEADGDFIDFIFGFLTIPLGSIIKTLIPNNFPGCVGNLYNSVKNTTRKIGFCDGNL*