AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018728.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018728.01.T01 MTR_4g017610 86.170 376 51 1 1 375 100 475 0.0 649
MsG0480018728.01.T01 MTR_4g017550 61.404 399 128 8 1 377 77 471 1.73e-152 439
MsG0480018728.01.T01 MTR_4g017830 56.543 405 150 6 1 379 97 501 3.24e-145 422
MsG0480018728.01.T01 MTR_4g017830 27.074 229 140 6 173 377 15 240 4.49e-11 64.7
MsG0480018728.01.T01 MTR_4g017410 57.921 404 128 7 1 379 98 484 1.66e-144 420
MsG0480018728.01.T01 MTR_4g017410 27.311 238 126 10 173 372 12 240 8.52e-11 63.9
MsG0480018728.01.T01 MTR_4g017440 57.750 400 146 6 1 379 105 502 1.12e-142 416
MsG0480018728.01.T01 MTR_4g019340 58.398 387 132 6 1 369 128 503 2.53e-142 414
MsG0480018728.01.T01 MTR_4g019340 26.829 246 143 6 166 377 34 276 3.93e-11 65.1
MsG0480018728.01.T01 MTR_4g017470 51.225 408 132 7 1 379 101 470 8.73e-119 354
MsG0480018728.01.T01 MTR_4g019310 38.710 403 216 11 1 379 95 490 1.02e-72 235
MsG0480018728.01.T01 MTR_4g019310 27.706 231 139 6 170 372 8 238 1.13e-11 66.6
MsG0480018728.01.T01 MTR_7g058710 32.381 420 230 12 1 383 79 481 7.16e-50 175
MsG0480018728.01.T01 MTR_7g058710 26.941 219 139 4 168 372 10 221 1.07e-15 79.0
MsG0480018728.01.T01 MTR_7g066570 33.976 415 219 15 1 383 93 484 1.14e-48 173
MsG0480018728.01.T01 MTR_7g066340 32.242 397 217 12 1 379 91 453 2.86e-48 171
MsG0480018728.01.T01 MTR_7g066340 27.354 223 145 5 167 372 9 231 6.28e-13 70.5
MsG0480018728.01.T01 MTR_7g066400 32.915 398 205 12 1 375 75 433 3.37e-48 170
MsG0480018728.01.T01 MTR_7g066260 31.436 404 217 13 1 379 94 462 1.77e-46 166
MsG0480018728.01.T01 MTR_7g066260 28.384 229 131 6 169 372 14 234 1.26e-14 75.5
MsG0480018728.01.T01 MTR_7g062280 32.151 423 231 14 1 386 89 492 9.09e-46 164
MsG0480018728.01.T01 MTR_7g066430 32.603 411 237 12 1 379 83 485 1.80e-45 164
MsG0480018728.01.T01 MTR_7g066350 32.323 396 209 9 1 375 88 445 6.34e-45 161
MsG0480018728.01.T01 MTR_7g066350 29.204 226 137 6 167 373 6 227 6.36e-18 85.5
MsG0480018728.01.T01 MTR_7g066410 31.818 440 229 14 1 382 89 515 6.38e-44 159
MsG0480018728.01.T01 MTR_7g012680 31.068 412 206 11 1 386 93 452 5.16e-43 156
MsG0480018728.01.T01 MTR_7g012680 27.500 200 125 6 167 351 11 205 3.68e-12 67.8
MsG0480018728.01.T01 MTR_7g062300 32.000 400 226 14 1 375 91 469 6.78e-40 148
MsG0480018728.01.T01 MTR_7g062300 29.268 246 133 12 171 388 13 245 4.38e-11 64.7
MsG0480018728.01.T01 MTR_1g041315 31.646 395 202 16 1 363 93 451 7.80e-38 142
MsG0480018728.01.T01 MTR_7g058700 29.197 411 194 14 1 383 584 925 1.96e-37 144
MsG0480018728.01.T01 MTR_7g063660 30.542 406 247 14 1 386 88 478 3.02e-37 140
MsG0480018728.01.T01 MTR_7g063660 32.370 173 100 5 170 329 9 177 1.34e-13 72.4
MsG0480018728.01.T01 MTR_7g063880 31.514 403 234 14 1 385 90 468 3.63e-37 140
MsG0480018728.01.T01 MTR_7g063900 30.166 421 239 12 1 386 89 489 1.01e-36 139
MsG0480018728.01.T01 MTR_7g063900 27.556 225 132 7 170 372 10 225 1.23e-11 66.6
MsG0480018728.01.T01 MTR_7g063890 30.244 410 246 11 1 383 106 502 2.54e-36 139
MsG0480018728.01.T01 MTR_7g066360 31.298 393 220 11 1 379 93 449 5.26e-36 137
MsG0480018728.01.T01 MTR_7g063820 30.918 414 242 16 1 386 88 485 7.87e-36 137
MsG0480018728.01.T01 MTR_7g063920 29.798 396 220 9 1 386 90 437 1.74e-35 135
MsG0480018728.01.T01 MTR_7g063840 27.628 409 250 12 1 386 89 474 1.10e-34 134
MsG0480018728.01.T01 MTR_7g066280 28.266 421 229 17 1 379 1 390 1.86e-34 132
MsG0480018728.01.T01 MTR_7g066580 28.851 409 218 13 1 376 74 442 2.17e-34 132
MsG0480018728.01.T01 MTR_7g066290 30.000 400 199 10 1 379 94 433 1.87e-33 129
MsG0480018728.01.T01 MTR_3g065200 27.073 410 219 13 1 385 72 426 7.08e-33 128
MsG0480018728.01.T01 MTR_7g063670 27.737 411 236 15 2 386 83 458 4.33e-29 118
MsG0480018728.01.T01 MTR_3g007740 27.720 386 251 9 2 379 77 442 8.18e-28 114
MsG0480018728.01.T01 MTR_7g063830 28.429 401 214 14 1 379 89 438 5.60e-27 112
MsG0480018728.01.T01 MTR_7g066480 28.070 399 195 12 1 379 95 421 1.61e-26 110
MsG0480018728.01.T01 MTR_7g066480 26.577 222 138 8 169 372 15 229 3.92e-11 64.7
MsG0480018728.01.T01 MTR_7g066680 31.034 261 156 5 117 358 31 286 2.78e-25 105
MsG0480018728.01.T01 MTR_7g066310 31.604 212 124 4 169 379 192 383 1.60e-23 101
MsG0480018728.01.T01 MTR_1g014060 31.884 207 111 5 192 386 203 391 3.16e-20 92.0
MsG0480018728.01.T01 MTR_1g014060 32.886 149 85 4 1 142 79 219 3.88e-12 67.8
MsG0480018728.01.T01 MTR_7g063720 30.303 231 125 5 160 375 13 222 1.19e-19 87.8
MsG0480018728.01.T01 MTR_7g066490 30.165 242 134 8 1 232 95 311 7.40e-18 85.1
MsG0480018728.01.T01 MTR_8g445860 28.139 231 128 9 170 373 13 232 5.22e-16 77.4
MsG0480018728.01.T01 MTR_2g029400 24.750 400 179 14 1 367 93 403 1.47e-15 78.2
MsG0480018728.01.T01 MTR_1g115440 27.311 238 126 7 169 373 10 233 1.54e-14 73.2
MsG0480018728.01.T01 MTR_4g019330 32.432 185 77 5 184 367 32 169 1.77e-14 71.6
MsG0480018728.01.T01 MTR_8g023240 23.977 342 212 10 59 371 159 481 2.68e-14 74.7
MsG0480018728.01.T01 MTR_0150s0010 28.761 226 138 7 169 373 9 232 7.21e-14 71.2
MsG0480018728.01.T01 MTR_7g100420 27.957 279 156 9 123 375 134 393 7.38e-13 70.1
MsG0480018728.01.T01 MTR_8g445920 42.857 63 36 0 169 231 9 71 3.87e-11 60.1
MsG0480018728.01.T01 MTR_8g044890 42.857 63 36 0 169 231 9 71 3.87e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018728.01.T01 AT5G01120 29.353 402 253 12 1 375 88 485 1.42e-38 145
MsG0480018728.01.T01 AT5G01140 30.946 391 239 12 1 375 72 447 1.92e-38 144
MsG0480018728.01.T01 AT3G09140 29.756 410 245 13 1 375 88 489 2.09e-38 144
MsG0480018728.01.T01 AT3G09140 26.471 238 143 8 167 372 6 243 6.28e-13 70.5
MsG0480018728.01.T01 AT5G43240 30.340 412 240 17 1 375 88 489 2.35e-35 136
MsG0480018728.01.T01 AT5G43240 30.340 412 240 17 1 375 88 489 2.35e-35 136
MsG0480018728.01.T01 AT5G43240 30.340 412 240 17 1 375 88 489 2.35e-35 136
MsG0480018728.01.T01 AT3G09140 27.626 438 246 14 1 375 88 517 7.43e-33 129
MsG0480018728.01.T01 AT5G01130 28.608 395 228 14 1 379 88 444 4.06e-32 126
MsG0480018728.01.T01 AT5G37320 28.606 409 244 14 1 379 89 479 5.91e-29 118
MsG0480018728.01.T01 AT5G01150 26.933 401 254 10 1 375 88 475 4.07e-28 115
MsG0480018728.01.T01 AT5G01150 26.754 228 124 8 170 367 9 223 4.91e-12 67.8
MsG0480018728.01.T01 AT3G09110 32.031 256 155 8 2 246 89 336 5.84e-28 113
MsG0480018728.01.T01 AT5G43240 29.885 348 206 13 58 375 6 345 3.55e-26 108
MsG0480018728.01.T01 AT3G09120 31.950 241 136 9 1 224 84 313 1.64e-19 89.0
MsG0480018728.01.T01 AT3G09120 26.872 227 146 5 167 373 3 229 1.25e-14 74.7

Find 84 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCTTACGAATACAATTT+TGG 0.148306 4:-9051331 MsG0480018728.01.T01:CDS
GAATCCCCTACTTATTATTA+TGG 0.206471 4:-9050964 MsG0480018728.01.T01:CDS
TAATAGACAACCTAAGAATT+TGG 0.262420 4:-9052091 MsG0480018728.01.T01:CDS
CAATTATAAGATAAATAAAA+TGG 0.268027 4:-9051247 MsG0480018728.01.T01:CDS
CAGGGGAATCATGCCTCTAA+TGG 0.286513 4:-9052057 MsG0480018728.01.T01:CDS
GGGTATTAAAGTCTTTATAA+TGG 0.290144 4:+9051119 None:intergenic
AGTTTCCTTACCATTCCATT+AGG 0.290188 4:-9051144 MsG0480018728.01.T01:CDS
CCCTCAGAGTCCAAATTCTT+AGG 0.309465 4:+9052081 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.310647 4:+9052093 None:intergenic
ATTCTTCTCCATCAACATAT+TGG 0.310876 4:+9051274 None:intergenic
ATGCAGATGTTGCTGGAATC+AGG 0.320991 4:-9051955 MsG0480018728.01.T01:CDS
TCCAAAATTGTATTCGTAAG+AGG 0.327361 4:+9051330 None:intergenic
TGTATCAAACACCCCATTAG+AGG 0.344943 4:+9052044 None:intergenic
CACATGTATCAATTGTTAAT+CGG 0.352686 4:-9050882 MsG0480018728.01.T01:CDS
ATATGTTGATGGAGAAGAAT+TGG 0.357603 4:-9051271 MsG0480018728.01.T01:CDS
CAGACATAAAACTTACTTGA+TGG 0.362318 4:+9052174 None:intergenic
GTTTCCTTACCATTCCATTA+GGG 0.375690 4:-9051143 MsG0480018728.01.T01:CDS
AGGGGAATCATGCCTCTAAT+GGG 0.380156 4:-9052056 MsG0480018728.01.T01:CDS
AACGTCTAAGTCCACTAGTC+AGG 0.385798 4:+9050540 None:intergenic
GAACTCAATGCAGATGTTGC+TGG 0.386915 4:-9051962 MsG0480018728.01.T01:CDS
GAATTTGGACTCTGAGGGTC+AGG 0.397162 4:-9052076 MsG0480018728.01.T01:CDS
GTCTTTATAATGGACCCTAA+TGG 0.418519 4:+9051129 None:intergenic
TCTTACCTCCCTTTCATCAA+TGG 0.421763 4:+9050687 None:intergenic
AAGAGGCTCATGAGAGGTTA+AGG 0.445820 4:+9051347 None:intergenic
AATCCTACAGCACCTTCTCT+TGG 0.447362 4:+9050838 None:intergenic
ATTCTCTTCCATTTGAAAGC+TGG 0.450179 4:+9050512 None:intergenic
TGAATACCATAATAATAAGT+AGG 0.466481 4:+9050958 None:intergenic
GAGAGGTTAAGGTATACTTC+AGG 0.468779 4:+9051358 None:intergenic
TATGTTGATGGAGAAGAATT+GGG 0.477595 4:-9051270 MsG0480018728.01.T01:CDS
GTACAACAGTGTGAAGAACT+TGG 0.479946 4:-9051067 MsG0480018728.01.T01:CDS
GAATACCATAATAATAAGTA+GGG 0.484408 4:+9050959 None:intergenic
ATCCTACAGCACCTTCTCTT+GGG 0.485199 4:+9050839 None:intergenic
CCACGGAACCAATATGTTGA+TGG 0.488534 4:-9051282 MsG0480018728.01.T01:CDS
CGTGATTACCATTGATGAAA+GGG 0.498975 4:-9050695 MsG0480018728.01.T01:CDS
ATTGAGTTCAGCAAGGAACT+TGG 0.501314 4:+9051976 None:intergenic
AACACATCCAGGAAAATTGT+TGG 0.509207 4:+9051098 None:intergenic
CACACAAAGCATTGGCAAGA+AGG 0.511399 4:-9051902 MsG0480018728.01.T01:CDS
CGAAACCTTGACTGCACCTG+CGG 0.512942 4:-9052123 MsG0480018728.01.T01:CDS
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4:+9052096 None:intergenic
AGAGCAGCATTATATGTAGT+AGG 0.519344 4:-9050808 MsG0480018728.01.T01:CDS
TTAATTCTTTCAGATATGAA+AGG 0.526183 4:+9050746 None:intergenic
CCATCAACATATTGGTTCCG+TGG 0.536459 4:+9051282 None:intergenic
TGCAGATGTTGCTGGAATCA+GGG 0.536790 4:-9051954 MsG0480018728.01.T01:CDS
TACAAATTACCAACACATCC+AGG 0.541284 4:+9051087 None:intergenic
ACACATCCAGGAAAATTGTT+GGG 0.541913 4:+9051099 None:intergenic
ATCATCATTGCAGAAGCTGA+TGG 0.549559 4:-9051189 MsG0480018728.01.T01:CDS
ATTCATGTGAATCACTATGC+AGG 0.552422 4:-9052227 MsG0480018728.01.T01:CDS
CCGTGATTACCATTGATGAA+AGG 0.554883 4:-9050696 MsG0480018728.01.T01:CDS
TCAAAATTTACCCTGACTAG+TGG 0.555267 4:-9050551 MsG0480018728.01.T01:CDS
TGATGATTATAATACAATAG+AGG 0.560654 4:-9050920 MsG0480018728.01.T01:CDS
TTCTAAAGGATGAATGTCAA+GGG 0.563362 4:+9050984 None:intergenic
GCAAAGTGTGGTGCTATACA+AGG 0.564905 4:+9051021 None:intergenic
ACCTAAGAATTTGGACTCTG+AGG 0.575501 4:-9052082 MsG0480018728.01.T01:CDS
AGTGATTCACATGAATTCCT+CGG 0.577391 4:+9052234 None:intergenic
TGGACCCTAATGGAATGGTA+AGG 0.578424 4:+9051139 None:intergenic
TATAATGGACCCTAATGGAA+TGG 0.581198 4:+9051134 None:intergenic
AGGAAAGAATTAGCAGCAAG+TGG 0.583512 4:+9050766 None:intergenic
TAATAAGTAGGGGATTCTAA+AGG 0.590723 4:+9050970 None:intergenic
ACGTCTAAGTCCACTAGTCA+GGG 0.592093 4:+9050541 None:intergenic
CCTAAGAATTTGGACTCTGA+GGG 0.592717 4:-9052081 MsG0480018728.01.T01:CDS
CTTCAAGATCATCAAGAGGA+AGG 0.593465 4:+9050722 None:intergenic
AATTTGGACTCTGAGGGTCA+GGG 0.595588 4:-9052075 MsG0480018728.01.T01:CDS
ATTTGGACTCTGAGGGTCAG+GGG 0.598326 4:-9052074 MsG0480018728.01.T01:CDS
CCTTTCATCAATGGTAATCA+CGG 0.598872 4:+9050696 None:intergenic
GAGGAAGTCACACAAAGCAT+TGG 0.599245 4:-9051910 MsG0480018728.01.T01:CDS
GGATGGAGCAACTCAGACAC+GGG 0.602700 4:+9051042 None:intergenic
ATTCTAAAGGATGAATGTCA+AGG 0.609187 4:+9050983 None:intergenic
ATTGAACTTGACCCAAGAGA+AGG 0.610689 4:-9050850 MsG0480018728.01.T01:CDS
AGGTTAAGGTATACTTCAGG+AGG 0.621580 4:+9051361 None:intergenic
AGGATGGAGCAACTCAGACA+CGG 0.622529 4:+9051041 None:intergenic
TTCAGACTCAACGCAACTAG+AGG 0.625063 4:-9051929 MsG0480018728.01.T01:CDS
AAGAAATGTTGCTGCATCCG+AGG 0.627559 4:-9052251 None:intergenic
AGTGTGGTGCTATACAAGGA+TGG 0.638386 4:+9051025 None:intergenic
GGGGAATCATGCCTCTAATG+GGG 0.639572 4:-9052055 MsG0480018728.01.T01:CDS
TTGATGTGAAGAATGCTCGA+AGG 0.642051 4:+9050570 None:intergenic
CAAAGAACAATGACGAACCA+CGG 0.642115 4:-9051299 MsG0480018728.01.T01:CDS
ACAAAGCATTGGCAAGAAGG+AGG 0.647102 4:-9051899 MsG0480018728.01.T01:intron
GACCCAAGAGAAGGTGCTGT+AGG 0.653060 4:-9050841 MsG0480018728.01.T01:CDS
GATTACCATTGATGAAAGGG+AGG 0.668082 4:-9050692 MsG0480018728.01.T01:intron
TCTAAAGGATGAATGTCAAG+GGG 0.670617 4:+9050985 None:intergenic
GTCACTTCAAGATCATCAAG+AGG 0.671503 4:+9050718 None:intergenic
AATACCATAATAATAAGTAG+GGG 0.676299 4:+9050960 None:intergenic
ATTCGTAAGAGGCTCATGAG+AGG 0.692406 4:+9051341 None:intergenic
CATCTGCATTGAGTTCAGCA+AGG 0.698278 4:+9051969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATAAGATAAATAAAA+TGG - Chr4:9051495-9051514 MsG0480018728.01.T01:intron 10.0%
!! ATAAAATGTTAAAACACAAA+TGG + Chr4:9051349-9051368 None:intergenic 15.0%
!!! AAATTTAAAATCATAGGTTA+AGG + Chr4:9050987-9051006 None:intergenic 15.0%
!!! TAACCTATGATTTTAAATTT+AGG - Chr4:9050987-9051006 MsG0480018728.01.T01:CDS 15.0%
!! AATAAAGAGAAATAGAACTT+TGG + Chr4:9051280-9051299 None:intergenic 20.0%
!! AATACCATAATAATAAGTAG+GGG + Chr4:9051785-9051804 None:intergenic 20.0%
!! AGGTTAAAAAAACAGAAAAA+AGG + Chr4:9052114-9052133 None:intergenic 20.0%
!! GAATACCATAATAATAAGTA+GGG + Chr4:9051786-9051805 None:intergenic 20.0%
!! GGATATATATAGTTAATACT+TGG - Chr4:9051129-9051148 MsG0480018728.01.T01:CDS 20.0%
!! TGAATACCATAATAATAAGT+AGG + Chr4:9051787-9051806 None:intergenic 20.0%
!! TGATGATTATAATACAATAG+AGG - Chr4:9051822-9051841 MsG0480018728.01.T01:intron 20.0%
!! TTAATTCTTTCAGATATGAA+AGG + Chr4:9051999-9052018 None:intergenic 20.0%
! CACATGTATCAATTGTTAAT+CGG - Chr4:9051860-9051879 MsG0480018728.01.T01:intron 25.0%
! GGAAAAAAAATCAATGGAAT+GGG + Chr4:9050927-9050946 None:intergenic 25.0%
! GGGTATTAAAGTCTTTATAA+TGG + Chr4:9051626-9051645 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG - Chr4:9050651-9050670 MsG0480018728.01.T01:intron 25.0%
! TGGAAAAAAAATCAATGGAA+TGG + Chr4:9050928-9050947 None:intergenic 25.0%
! TTTCGTTGTGAATCTATATT+GGG - Chr4:9051015-9051034 MsG0480018728.01.T01:CDS 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG + Chr4:9050652-9050671 None:intergenic 25.0%
!! GCACCTAAATTTAAAATCAT+AGG + Chr4:9050993-9051012 None:intergenic 25.0%
!! TATCTAAAACCATTACATTG+TGG - Chr4:9051085-9051104 MsG0480018728.01.T01:CDS 25.0%
AAGAGAAATAGAACTTTGGT+AGG + Chr4:9051276-9051295 None:intergenic 30.0%
ACAATTAAAGGGAATCAAAG+AGG + Chr4:9052134-9052153 None:intergenic 30.0%
AGAGAAATAGAACTTTGGTA+GGG + Chr4:9051275-9051294 None:intergenic 30.0%
ATCTATATTGGGGTAAAATG+CGG - Chr4:9051026-9051045 MsG0480018728.01.T01:CDS 30.0%
ATGTTATCTGTTAGTGATAG+AGG - Chr4:9051167-9051186 MsG0480018728.01.T01:CDS 30.0%
ATTCTTCTCCATCAACATAT+TGG + Chr4:9051471-9051490 None:intergenic 30.0%
CAAATGGTTAGAATGAAAGT+TGG + Chr4:9051333-9051352 None:intergenic 30.0%
CAGACATAAAACTTACTTGA+TGG + Chr4:9050571-9050590 None:intergenic 30.0%
CTGCAATGACAATATTTGTT+AGG - Chr4:9051108-9051127 MsG0480018728.01.T01:CDS 30.0%
GAATCCCCTACTTATTATTA+TGG - Chr4:9051778-9051797 MsG0480018728.01.T01:intron 30.0%
GCAAGTGGAAAAAAAATCAA+TGG + Chr4:9050933-9050952 None:intergenic 30.0%
GTTTCGTTGTGAATCTATAT+TGG - Chr4:9051014-9051033 MsG0480018728.01.T01:CDS 30.0%
TAATAAGTAGGGGATTCTAA+AGG + Chr4:9051775-9051794 None:intergenic 30.0%
TCCAAAATTGTATTCGTAAG+AGG + Chr4:9051415-9051434 None:intergenic 30.0%
TCTTTGATTCCCTTTAATTG+TGG - Chr4:9052133-9052152 MsG0480018728.01.T01:CDS 30.0%
TTCGTTGTGAATCTATATTG+GGG - Chr4:9051016-9051035 MsG0480018728.01.T01:CDS 30.0%
! ATATGTTGATGGAGAAGAAT+TGG - Chr4:9051471-9051490 MsG0480018728.01.T01:intron 30.0%
! ATTCTAAAGGATGAATGTCA+AGG + Chr4:9051762-9051781 None:intergenic 30.0%
! TATGTTGATGGAGAAGAATT+GGG - Chr4:9051472-9051491 MsG0480018728.01.T01:intron 30.0%
! TTAATACCCAACAATTTTCC+TGG - Chr4:9051637-9051656 MsG0480018728.01.T01:intron 30.0%
! TTCTAAAGGATGAATGTCAA+GGG + Chr4:9051761-9051780 None:intergenic 30.0%
!!! GTTTGTTGTGTTTCCATTTT+AGG - Chr4:9050891-9050910 MsG0480018728.01.T01:CDS 30.0%
!!! GTTTTTTACCAGCTTTCAAA+TGG - Chr4:9052222-9052241 MsG0480018728.01.T01:CDS 30.0%
AAAGCCTACACCACAATTAA+AGG + Chr4:9052146-9052165 None:intergenic 35.0%
AACACATCCAGGAAAATTGT+TGG + Chr4:9051647-9051666 None:intergenic 35.0%
AAGCCTACACCACAATTAAA+GGG + Chr4:9052145-9052164 None:intergenic 35.0%
ACACATCCAGGAAAATTGTT+GGG + Chr4:9051646-9051665 None:intergenic 35.0%
AGAGCAGCATTATATGTAGT+AGG - Chr4:9051934-9051953 MsG0480018728.01.T01:CDS 35.0%
AGTGATTCACATGAATTCCT+CGG + Chr4:9050511-9050530 None:intergenic 35.0%
AGTTTCCTTACCATTCCATT+AGG - Chr4:9051598-9051617 MsG0480018728.01.T01:intron 35.0%
ATTCATGTGAATCACTATGC+AGG - Chr4:9050515-9050534 MsG0480018728.01.T01:CDS 35.0%
ATTCCCTTTAATTGTGGTGT+AGG - Chr4:9052139-9052158 MsG0480018728.01.T01:CDS 35.0%
ATTCTCTTCCATTTGAAAGC+TGG + Chr4:9052233-9052252 None:intergenic 35.0%
CCTTTCATCAATGGTAATCA+CGG + Chr4:9052049-9052068 None:intergenic 35.0%
CGTGATTACCATTGATGAAA+GGG - Chr4:9052047-9052066 MsG0480018728.01.T01:CDS 35.0%
GTTTCCTTACCATTCCATTA+GGG - Chr4:9051599-9051618 MsG0480018728.01.T01:intron 35.0%
TACAAATTACCAACACATCC+AGG + Chr4:9051658-9051677 None:intergenic 35.0%
TCAAAATTTACCCTGACTAG+TGG - Chr4:9052191-9052210 MsG0480018728.01.T01:CDS 35.0%
TCTAAAGGATGAATGTCAAG+GGG + Chr4:9051760-9051779 None:intergenic 35.0%
! AACAATTTTCCTGGATGTGT+TGG - Chr4:9051646-9051665 MsG0480018728.01.T01:intron 35.0%
! GCCTCTTACGAATACAATTT+TGG - Chr4:9051411-9051430 MsG0480018728.01.T01:intron 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG + Chr4:9050649-9050668 None:intergenic 35.0%
!! ATAGAGGTGTCATTAGGAAA+GGG - Chr4:9051183-9051202 MsG0480018728.01.T01:CDS 35.0%
!! CTTGTTTCTACAATGACCTA+AGG + Chr4:9050962-9050981 None:intergenic 35.0%
!! GTCTTTATAATGGACCCTAA+TGG + Chr4:9051616-9051635 None:intergenic 35.0%
!! TATAATGGACCCTAATGGAA+TGG + Chr4:9051611-9051630 None:intergenic 35.0%
!! TTAGTGATAGAGGTGTCATT+AGG - Chr4:9051177-9051196 MsG0480018728.01.T01:CDS 35.0%
ACCTAAGAATTTGGACTCTG+AGG - Chr4:9050660-9050679 MsG0480018728.01.T01:intron 40.0%
AGGAAAGAATTAGCAGCAAG+TGG + Chr4:9051979-9051998 None:intergenic 40.0%
AGGTTAAGGTATACTTCAGG+AGG + Chr4:9051384-9051403 None:intergenic 40.0%
AGTTAGAACTTAGAGGAGAC+TGG - Chr4:9051210-9051229 MsG0480018728.01.T01:CDS 40.0%
ATCATCATTGCAGAAGCTGA+TGG - Chr4:9051553-9051572 MsG0480018728.01.T01:intron 40.0%
ATTGAACTTGACCCAAGAGA+AGG - Chr4:9051892-9051911 MsG0480018728.01.T01:intron 40.0%
ATTGAGTTCAGCAAGGAACT+TGG + Chr4:9050769-9050788 None:intergenic 40.0%
CAAAGAACAATGACGAACCA+CGG - Chr4:9051443-9051462 MsG0480018728.01.T01:intron 40.0%
CCGTGATTACCATTGATGAA+AGG - Chr4:9052046-9052065 MsG0480018728.01.T01:CDS 40.0%
CCTAAGAATTTGGACTCTGA+GGG - Chr4:9050661-9050680 MsG0480018728.01.T01:intron 40.0%
CTTCAAGATCATCAAGAGGA+AGG + Chr4:9052023-9052042 None:intergenic 40.0%
GAAATAGAACTTTGGTAGGG+TGG + Chr4:9051272-9051291 None:intergenic 40.0%
GAGAGGTTAAGGTATACTTC+AGG + Chr4:9051387-9051406 None:intergenic 40.0%
GATTACCATTGATGAAAGGG+AGG - Chr4:9052050-9052069 MsG0480018728.01.T01:CDS 40.0%
GGGAACTAGTTAGAACTTAG+AGG - Chr4:9051203-9051222 MsG0480018728.01.T01:CDS 40.0%
GTACAACAGTGTGAAGAACT+TGG - Chr4:9051675-9051694 MsG0480018728.01.T01:intron 40.0%
GTCACTTCAAGATCATCAAG+AGG + Chr4:9052027-9052046 None:intergenic 40.0%
TACCTCGACAACGACATTAA+TGG + Chr4:9051067-9051086 None:intergenic 40.0%
TCAATGGAATGGGTGCATAA+TGG + Chr4:9050917-9050936 None:intergenic 40.0%
TCATTGCAGCCACAATGTAA+TGG + Chr4:9051097-9051116 None:intergenic 40.0%
TCTTACCTCCCTTTCATCAA+TGG + Chr4:9052058-9052077 None:intergenic 40.0%
TGTATCAAACACCCCATTAG+AGG + Chr4:9050701-9050720 None:intergenic 40.0%
TTCCACTTGCAAATGACCTT+AGG - Chr4:9050943-9050962 MsG0480018728.01.T01:CDS 40.0%
TTGATGAAAGGGAGGTAAGA+TGG - Chr4:9052058-9052077 MsG0480018728.01.T01:CDS 40.0%
TTGATGTGAAGAATGCTCGA+AGG + Chr4:9052175-9052194 None:intergenic 40.0%
! TGCTTTTGACAAGCAAAGTG+TGG + Chr4:9051736-9051755 None:intergenic 40.0%
!! GATAGAGGTGTCATTAGGAA+AGG - Chr4:9051182-9051201 MsG0480018728.01.T01:CDS 40.0%
AACGTCTAAGTCCACTAGTC+AGG + Chr4:9052205-9052224 None:intergenic 45.0%
AAGAGGCTCATGAGAGGTTA+AGG + Chr4:9051398-9051417 None:intergenic 45.0%
AATCCTACAGCACCTTCTCT+TGG + Chr4:9051907-9051926 None:intergenic 45.0%
ACGTCTAAGTCCACTAGTCA+GGG + Chr4:9052204-9052223 None:intergenic 45.0%
ACTTTGGTAGGGTGGAACAA+TGG + Chr4:9051264-9051283 None:intergenic 45.0%
AGGGGAATCATGCCTCTAAT+GGG - Chr4:9050686-9050705 MsG0480018728.01.T01:intron 45.0%
ATCCTACAGCACCTTCTCTT+GGG + Chr4:9051906-9051925 None:intergenic 45.0%
ATGCAGATGTTGCTGGAATC+AGG - Chr4:9050787-9050806 MsG0480018728.01.T01:CDS 45.0%
ATTCGTAAGAGGCTCATGAG+AGG + Chr4:9051404-9051423 None:intergenic 45.0%
CATCTGCATTGAGTTCAGCA+AGG + Chr4:9050776-9050795 None:intergenic 45.0%
CCACGGAACCAATATGTTGA+TGG - Chr4:9051460-9051479 MsG0480018728.01.T01:intron 45.0%
CCCTCAGAGTCCAAATTCTT+AGG + Chr4:9050664-9050683 None:intergenic 45.0%
GAACTCAATGCAGATGTTGC+TGG - Chr4:9050780-9050799 MsG0480018728.01.T01:CDS 45.0%
GACCTAAGGTCATTTGCAAG+TGG + Chr4:9050948-9050967 None:intergenic 45.0%
GAGGAAGTCACACAAAGCAT+TGG - Chr4:9050832-9050851 MsG0480018728.01.T01:CDS 45.0%
GGGTGCATAATGGCCTAAAA+TGG + Chr4:9050907-9050926 None:intergenic 45.0%
TGCAGATGTTGCTGGAATCA+GGG - Chr4:9050788-9050807 MsG0480018728.01.T01:CDS 45.0%
TGCCATTAATGTCGTTGTCG+AGG - Chr4:9051062-9051081 MsG0480018728.01.T01:CDS 45.0%
TGGACCCTAATGGAATGGTA+AGG + Chr4:9051606-9051625 None:intergenic 45.0%
TGGCAAGAAGGAGGTAACTA+AGG - Chr4:9050852-9050871 MsG0480018728.01.T01:CDS 45.0%
TTCAGACTCAACGCAACTAG+AGG - Chr4:9050813-9050832 MsG0480018728.01.T01:CDS 45.0%
! AATTTGGACTCTGAGGGTCA+GGG - Chr4:9050667-9050686 MsG0480018728.01.T01:intron 45.0%
! TCTATTAGGAGGTTTTCCGC+AGG + Chr4:9050638-9050657 None:intergenic 45.0%
!! ACAAAGCATTGGCAAGAAGG+AGG - Chr4:9050843-9050862 MsG0480018728.01.T01:CDS 45.0%
!! AGTGTGGTGCTATACAAGGA+TGG + Chr4:9051720-9051739 None:intergenic 45.0%
!! CACACAAAGCATTGGCAAGA+AGG - Chr4:9050840-9050859 MsG0480018728.01.T01:CDS 45.0%
!! CCATCAACATATTGGTTCCG+TGG + Chr4:9051463-9051482 None:intergenic 45.0%
!! GCAAAGTGTGGTGCTATACA+AGG + Chr4:9051724-9051743 None:intergenic 45.0%
AGGATGGAGCAACTCAGACA+CGG + Chr4:9051704-9051723 None:intergenic 50.0%
CAGGGGAATCATGCCTCTAA+TGG - Chr4:9050685-9050704 MsG0480018728.01.T01:intron 50.0%
GGGGAATCATGCCTCTAATG+GGG - Chr4:9050687-9050706 MsG0480018728.01.T01:intron 50.0%
! ATTTGGACTCTGAGGGTCAG+GGG - Chr4:9050668-9050687 MsG0480018728.01.T01:intron 50.0%
! GAATTTGGACTCTGAGGGTC+AGG - Chr4:9050666-9050685 MsG0480018728.01.T01:intron 50.0%
CGAAACCTTGACTGCACCTG+CGG - Chr4:9050619-9050638 MsG0480018728.01.T01:intron 55.0%
GGATGGAGCAACTCAGACAC+GGG + Chr4:9051703-9051722 None:intergenic 55.0%
! GTTTTCCGCAGGTGCAGTCA+AGG + Chr4:9050627-9050646 None:intergenic 55.0%
!! GACCCAAGAGAAGGTGCTGT+AGG - Chr4:9051901-9051920 MsG0480018728.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 9050496 9052268 9050496 ID=MsG0480018728.01;Name=MsG0480018728.01
Chr4 mRNA 9050496 9052268 9050496 ID=MsG0480018728.01.T01;Parent=MsG0480018728.01;Name=MsG0480018728.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|0.5|0.66|3|0|391
Chr4 exon 9051900 9052268 9051900 ID=MsG0480018728.01.T01:exon:2224;Parent=MsG0480018728.01.T01
Chr4 exon 9050693 9051391 9050693 ID=MsG0480018728.01.T01:exon:2223;Parent=MsG0480018728.01.T01
Chr4 exon 9050496 9050603 9050496 ID=MsG0480018728.01.T01:exon:2222;Parent=MsG0480018728.01.T01
Chr4 CDS 9051900 9052268 9051900 ID=MsG0480018728.01.T01:cds;Parent=MsG0480018728.01.T01
Chr4 CDS 9050693 9051391 9050693 ID=MsG0480018728.01.T01:cds;Parent=MsG0480018728.01.T01
Chr4 CDS 9050496 9050603 9050496 ID=MsG0480018728.01.T01:cds;Parent=MsG0480018728.01.T01
Gene Sequence

>MsG0480018728.01.T01

ATGTTGCTGCATCCGAGGAATTCATGTGAATCACTATGCAGGAAGTTGTACTTGAATATTGATGATTCTGAACCATCAAGTAAGTTTTATGTCTGTGATTCTTGTAACAAGTTCACTACTTTTCGAAACCTTGACTGCACCTGCGGAAAACCTCCTAATAGACAACCTAAGAATTTGGACTCTGAGGGTCAGGGGAATCATGCCTCTAATGGGGTGTTTGATACAGAAAGTGAACAAATGTTTCTAGTGTCTGATGATTTGAATATTGTGCCAAGTTCCTTGCTGAACTCAATGCAGATGTTGCTGGAATCAGGGATTTCAGACTCAACGCAACTAGAGGAAGTCACACAAAGCATTGGCAAGAAGGAGATATTGAACCTCCTGAAGTATACCTTAACCTCTCATGAGCCTCTTACGAATACAATTTTGGAAAGTAGCTCAAAGAACAATGACGAACCACGGAACCAATATGTTGATGGAGAAGAATTGGGCAATTATAAGATAAATAAAATGGATATTAAAGTATTGCAAAGCAAATCTCAGAATAAGATCATCATTGCAGAAGCTGATGGAGATTTTATTGACTTCATTTTTAGTTTCCTTACCATTCCATTAGGGTCCATTATAAAGACTTTAATACCCAACAATTTTCCTGGATGTGTTGGTAATTTGTACAACAGTGTGAAGAACTTGGATCCCGTGTCTGAGTTGCTCCATCCTTGTATAGCACCACACTTTGCTTGTCAAAAGCAACCCCTTGACATTCATCCTTTAGAATCCCCTACTTATTATTATGGTATTCATCACAATGATGATGATGATGATTATAATACAATAGAGGAAGTGATTTCTAATTCACATGTATCAATTGTTAATCGGATAAACGTGATTGAACTTGACCCAAGAGAAGGTGCTGTAGGATTTGTGAAGAGAGCAGCATTATATGTAGTAGGAGATGATCTAGAAGTAAAACCACTTGCTGCTAATTCTTTCCTTTCATATCTGAAAGAATTAAGCCTTCCTCTTGATGATCTTGAAGTGACCGTGATTACCATTGATGAAAGGGAGGCTTTGTGCTTCCTTCGAGCATTCTTCACATCAAAATTTACCCTGACTAGTGGACTTAGACGTTTTTTACCAGCTTTCAAATGGAAGAGAATTATCTATGAGTTTTAG
Protein sequence

>MsG0480018728.01.T01

MLLHPRNSCESLCRKLYLNIDDSEPSSKFYVCDSCNKFTTFRNLDCTCGKPPNRQPKNLDSEGQGNHASNGVFDTESEQMFLVSDDLNIVPSSLLNSMQMLLESGISDSTQLEEVTQSIGKKEILNLLKYTLTSHEPLTNTILESSSKNNDEPRNQYVDGEELGNYKINKMDIKVLQSKSQNKIIIAEADGDFIDFIFSFLTIPLGSIIKTLIPNNFPGCVGNLYNSVKNLDPVSELLHPCIAPHFACQKQPLDIHPLESPTYYYGIHHNDDDDDYNTIEEVISNSHVSIVNRINVIELDPREGAVGFVKRAALYVVGDDLEVKPLAANSFLSYLKELSLPLDDLEVTVITIDEREALCFLRAFFTSKFTLTSGLRRFLPAFKWKRIIYEF*