AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000552.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000552.01.T01 MTR_1g014240 98.039 102 2 0 1 102 577 678 1.76e-71 224
MsG0180000552.01.T01 MTR_4g093110 45.833 72 39 0 1 72 555 626 1.80e-17 76.3
MsG0180000552.01.T01 MTR_4g093140 45.833 72 39 0 1 72 555 626 1.82e-17 76.3
MsG0180000552.01.T01 MTR_4g093050 50.704 71 35 0 1 71 560 630 7.63e-17 74.7
MsG0180000552.01.T01 MTR_8g068050 47.059 68 36 0 1 68 458 525 8.98e-17 74.3
MsG0180000552.01.T01 MTR_8g068050 47.059 68 36 0 1 68 568 635 9.12e-17 74.3
MsG0180000552.01.T01 MTR_8g067735 45.588 68 37 0 1 68 573 640 1.44e-16 73.9
MsG0180000552.01.T01 MTR_4g093070 42.254 71 41 0 1 71 562 632 1.68e-16 73.6
MsG0180000552.01.T01 MTR_4g093080 40.845 71 42 0 1 71 562 632 1.97e-16 73.6
MsG0180000552.01.T01 MTR_8g067720 44.286 70 39 0 1 70 553 622 3.76e-16 72.8
MsG0180000552.01.T01 MTR_8g067930 36.275 102 52 2 1 97 554 647 4.61e-16 72.4
MsG0180000552.01.T01 MTR_8g067630 45.588 68 37 0 1 68 502 569 7.30e-16 71.6
MsG0180000552.01.T01 MTR_8g067690 35.000 100 54 2 1 100 570 658 1.95e-14 67.8
MsG0180000552.01.T01 MTR_4g093040 37.000 100 50 2 1 100 573 659 2.97e-14 67.4
MsG0180000552.01.T01 MTR_7g115740 37.500 88 55 0 4 91 499 586 3.68e-14 67.0
MsG0180000552.01.T01 MTR_7g115740 37.500 88 55 0 4 91 575 662 3.72e-14 67.0
MsG0180000552.01.T01 MTR_4g093040 37.000 100 50 2 1 100 491 577 3.78e-14 67.0
MsG0180000552.01.T01 MTR_3g460810 47.692 65 33 1 1 65 539 602 4.55e-14 66.6
MsG0180000552.01.T01 MTR_5g095970 36.047 86 42 2 1 73 572 657 9.02e-14 65.9
MsG0180000552.01.T01 MTR_3g007870 34.884 86 43 2 1 73 559 644 3.55e-13 64.3
MsG0180000552.01.T01 MTR_7g116130 45.000 60 33 0 2 61 570 629 1.14e-12 62.8
MsG0180000552.01.T01 MTR_1g099260 41.791 67 39 0 2 68 558 624 1.57e-12 62.4
MsG0180000552.01.T01 MTR_5g077100 39.706 68 41 0 1 68 586 653 6.48e-12 60.5
MsG0180000552.01.T01 MTR_5g025020 33.824 68 45 0 1 68 579 646 7.60e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000552.01.T01 AT5G03140 42.029 69 39 1 1 69 591 658 8.60e-14 65.9
MsG0180000552.01.T01 AT3G53810 42.466 73 40 2 1 71 561 633 1.69e-13 65.1
MsG0180000552.01.T01 AT5G55830 42.647 68 39 0 1 68 582 649 1.96e-13 65.1
MsG0180000552.01.T01 AT5G10530 43.478 69 38 1 4 71 552 620 2.30e-13 64.7
MsG0180000552.01.T01 AT3G53380 42.647 68 39 0 1 68 594 661 4.63e-13 63.9
MsG0180000552.01.T01 AT5G06740 38.095 105 61 3 1 102 549 652 9.27e-13 63.2
MsG0180000552.01.T01 AT5G06740 38.095 105 61 3 1 102 561 664 9.56e-13 63.2
MsG0180000552.01.T01 AT5G65600 41.791 67 38 1 4 69 573 639 2.13e-12 62.0
MsG0180000552.01.T01 AT2G37710 37.500 72 44 1 1 71 561 632 1.19e-11 59.7

Find 25 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGCTTATTGTAGGTTTA+TGG 0.178488 1:+7712875 MsG0180000552.01.T01:CDS
TTCCTTGATGAATTGGGTTT+GGG 0.254127 1:+7712774 None:intergenic
GGACCATTGATTTGCCTAAA+AGG 0.283416 1:-7713018 None:intergenic
TTTCATGTTCCTTGATGAAT+TGG 0.284388 1:+7712767 None:intergenic
ACTAACAGTCTTGTTACTTC+TGG 0.290183 1:+7713060 MsG0180000552.01.T01:CDS
TAAGGCTTATGAAGTAATTA+AGG 0.292782 1:+7712927 MsG0180000552.01.T01:CDS
GTTCCTTGATGAATTGGGTT+TGG 0.348317 1:+7712773 None:intergenic
TAAGGTTCTTCAACTTGAAA+TGG 0.357917 1:+7712945 MsG0180000552.01.T01:CDS
ATGAAGAGTTTGCTTATTGT+AGG 0.372487 1:+7712868 MsG0180000552.01.T01:CDS
TTCATGTTCCTTGATGAATT+GGG 0.382059 1:+7712768 None:intergenic
ATTTGCCTAAAAGGTACAAT+AGG 0.390367 1:-7713009 None:intergenic
TGCATATCAAGTGGAAGTTC+AGG 0.394428 1:-7712973 None:intergenic
TTGGGTTTGGGGACTTTATG+TGG 0.431991 1:+7712786 MsG0180000552.01.T01:CDS
GTCATAGGTGGTGCCATTGA+GGG 0.477311 1:-7713039 None:intergenic
GTAACAAGACTGTTAGTCAT+AGG 0.484343 1:-7713054 None:intergenic
CTCATTCAAATGATAAAGAA+AGG 0.500852 1:+7712902 MsG0180000552.01.T01:CDS
TTAATTACTTCATAAGCCTT+AGG 0.506325 1:-7712925 None:intergenic
AGTCATAGGTGGTGCCATTG+AGG 0.524601 1:-7713040 None:intergenic
TCCTTGATGAATTGGGTTTG+GGG 0.537881 1:+7712775 None:intergenic
GCAAATCAATGGTCCCTCAA+TGG 0.539122 1:+7713026 MsG0180000552.01.T01:CDS
TGCCTAAAAGGTACAATAGG+AGG 0.560063 1:-7713006 None:intergenic
GTTTGGGGACTTTATGTGGA+AGG 0.573280 1:+7712790 MsG0180000552.01.T01:CDS
AAATGATAAAGAAAGGCCTA+AGG 0.599723 1:+7712909 MsG0180000552.01.T01:CDS
GCACGATCATGCATATCAAG+TGG 0.608568 1:-7712982 None:intergenic
ACAAGACTGTTAGTCATAGG+TGG 0.653990 1:-7713051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTCATTCAAATGATAAAGAA+AGG + Chr1:7712902-7712921 MsG0180000552.01.T01:CDS 25.0%
! TAAGGCTTATGAAGTAATTA+AGG + Chr1:7712927-7712946 MsG0180000552.01.T01:CDS 25.0%
! TGCTGATGAAAAATTGAATA+TGG + Chr1:7712828-7712847 MsG0180000552.01.T01:CDS 25.0%
! TTAATTACTTCATAAGCCTT+AGG - Chr1:7712928-7712947 None:intergenic 25.0%
AAATGATAAAGAAAGGCCTA+AGG + Chr1:7712909-7712928 MsG0180000552.01.T01:CDS 30.0%
GTTTGCTTATTGTAGGTTTA+TGG + Chr1:7712875-7712894 MsG0180000552.01.T01:CDS 30.0%
TAAGGTTCTTCAACTTGAAA+TGG + Chr1:7712945-7712964 MsG0180000552.01.T01:CDS 30.0%
! ATGAAGAGTTTGCTTATTGT+AGG + Chr1:7712868-7712887 MsG0180000552.01.T01:CDS 30.0%
! ATTTGCCTAAAAGGTACAAT+AGG - Chr1:7713012-7713031 None:intergenic 30.0%
GTAACAAGACTGTTAGTCAT+AGG - Chr1:7713057-7713076 None:intergenic 35.0%
! GTACCTTTTAGGCAAATCAA+TGG + Chr1:7713015-7713034 MsG0180000552.01.T01:CDS 35.0%
!! ACTAACAGTCTTGTTACTTC+TGG + Chr1:7713060-7713079 MsG0180000552.01.T01:CDS 35.0%
ACAAGACTGTTAGTCATAGG+TGG - Chr1:7713054-7713073 None:intergenic 40.0%
TGCATATCAAGTGGAAGTTC+AGG - Chr1:7712976-7712995 None:intergenic 40.0%
! CTCCTCCTATTGTACCTTTT+AGG + Chr1:7713004-7713023 MsG0180000552.01.T01:CDS 40.0%
! TGCCTAAAAGGTACAATAGG+AGG - Chr1:7713009-7713028 None:intergenic 40.0%
!! GGACCATTGATTTGCCTAAA+AGG - Chr1:7713021-7713040 None:intergenic 40.0%
GCAAATCAATGGTCCCTCAA+TGG + Chr1:7713026-7713045 MsG0180000552.01.T01:CDS 45.0%
GCACGATCATGCATATCAAG+TGG - Chr1:7712985-7713004 None:intergenic 45.0%
GTTTGGGGACTTTATGTGGA+AGG + Chr1:7712790-7712809 MsG0180000552.01.T01:CDS 45.0%
TTGGGTTTGGGGACTTTATG+TGG + Chr1:7712786-7712805 MsG0180000552.01.T01:CDS 45.0%
!! AGTCATAGGTGGTGCCATTG+AGG - Chr1:7713043-7713062 None:intergenic 50.0%
!! GTCATAGGTGGTGCCATTGA+GGG - Chr1:7713042-7713061 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 7712781 7713089 7712781 ID=MsG0180000552.01;Name=MsG0180000552.01
Chr1 mRNA 7712781 7713089 7712781 ID=MsG0180000552.01.T01;Parent=MsG0180000552.01;Name=MsG0180000552.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|102
Chr1 exon 7712781 7713089 7712781 ID=MsG0180000552.01.T01:exon:2820;Parent=MsG0180000552.01.T01
Chr1 CDS 7712781 7713089 7712781 ID=MsG0180000552.01.T01:cds;Parent=MsG0180000552.01.T01
Gene Sequence

>MsG0180000552.01.T01

ATGAATTGGGTTTGGGGACTTTATGTGGAAGGAAATCTTATGTGTGCTGCTGATGAAAAATTGAATATGGAGTTTGATGTTAGTGAAATGAAGAGTTTGCTTATTGTAGGTTTATGGTGTACTCATTCAAATGATAAAGAAAGGCCTAAGGCTTATGAAGTAATTAAGGTTCTTCAACTTGAAATGGCATTACCTGAACTTCCACTTGATATGCATGATCGTGCTCCTCCTATTGTACCTTTTAGGCAAATCAATGGTCCCTCAATGGCACCACCTATGACTAACAGTCTTGTTACTTCTGGAAGATAG

Protein sequence

>MsG0180000552.01.T01

MNWVWGLYVEGNLMCAADEKLNMEFDVSEMKSLLIVGLWCTHSNDKERPKAYEVIKVLQLEMALPELPLDMHDRAPPIVPFRQINGPSMAPPMTNSLVTSGR*