AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037964.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037964.01.T01 MTR_8g098595 74.790 119 16 2 15 133 1 105 1.14e-57 176
MsG0780037964.01.T01 MTR_8g028920 70.588 119 21 2 15 133 16 120 9.20e-55 170
MsG0780037964.01.T01 MTR_4g106690 70.588 119 21 3 15 133 16 120 2.08e-52 164
MsG0780037964.01.T01 MTR_8g028920 86.250 80 11 0 54 133 2 81 1.05e-47 150
MsG0780037964.01.T01 MTR_1g073600 81.250 80 15 0 38 117 18 97 9.67e-44 140
MsG0780037964.01.T01 MTR_8g098615 60.241 83 19 3 12 94 4 72 2.75e-24 89.4
MsG0780037964.01.T01 MTR_8g098615 62.025 79 16 3 16 94 1 65 8.99e-24 87.8
MsG0780037964.01.T01 MTR_8g098635 60.241 83 19 3 11 93 2 70 1.36e-21 88.6
MsG0780037964.01.T01 MTR_1g073390 51.786 112 29 7 15 125 1 88 2.54e-21 82.0
MsG0780037964.01.T01 MTR_2g078010 34.831 89 56 2 45 133 37 123 1.84e-15 68.6
MsG0780037964.01.T01 MTR_2g078010 34.831 89 56 2 45 133 37 123 2.88e-15 68.2
MsG0780037964.01.T01 MTR_2g078010 34.722 72 46 1 45 116 37 107 7.35e-14 63.5
MsG0780037964.01.T01 MTR_3g098780 32.927 82 54 1 51 132 41 121 6.53e-13 62.8
MsG0780037964.01.T01 MTR_3g098780 32.927 82 54 1 51 132 65 145 7.00e-13 63.2
MsG0780037964.01.T01 MTR_8g468530 25.984 127 78 4 20 132 13 137 7.10e-13 62.4
MsG0780037964.01.T01 MTR_4g108080 29.213 89 61 2 44 132 35 121 8.57e-13 61.6
MsG0780037964.01.T01 MTR_4g108080 30.137 73 50 1 44 116 35 106 2.74e-12 59.7
MsG0780037964.01.T01 MTR_5g075580 30.337 89 60 2 45 133 4 90 5.43e-12 58.9
MsG0780037964.01.T01 MTR_2g078010 36.486 74 45 2 60 133 66 137 6.16e-12 59.7
MsG0780037964.01.T01 MTR_7g116940 31.579 95 58 3 45 133 33 126 8.43e-12 59.3
MsG0780037964.01.T01 MTR_7g116940 30.337 89 60 2 45 133 33 119 8.49e-12 58.9
MsG0780037964.01.T01 MTR_8g071040 30.337 89 60 2 45 133 33 119 1.35e-11 58.5
MsG0780037964.01.T01 MTR_1g021150 30.337 89 60 2 45 133 33 119 1.87e-11 58.2
MsG0780037964.01.T01 MTR_4g073240 29.213 89 61 2 45 133 4 90 2.06e-11 57.4
MsG0780037964.01.T01 MTR_4g073240 29.213 89 61 2 45 133 33 119 2.65e-11 57.8
MsG0780037964.01.T01 MTR_3g110110 29.885 87 60 1 47 133 39 124 3.40e-11 57.8
MsG0780037964.01.T01 MTR_1g021150 30.337 89 60 2 45 133 41 127 3.75e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037964.01.T01 AT1G63800 70.588 119 21 3 15 133 16 120 3.51e-53 166
MsG0780037964.01.T01 AT1G63800 70.588 119 21 3 15 133 1 105 4.03e-53 165
MsG0780037964.01.T01 AT5G41340 68.908 119 23 3 15 133 16 120 1.28e-51 162
MsG0780037964.01.T01 AT2G46030 62.500 120 31 2 14 133 18 123 1.08e-46 149
MsG0780037964.01.T01 AT2G46030 62.500 120 31 2 14 133 18 123 1.08e-46 149
MsG0780037964.01.T01 AT2G46030 63.559 118 29 2 15 132 16 119 1.31e-46 149
MsG0780037964.01.T01 AT2G46030 63.559 118 29 2 15 132 16 119 1.31e-46 149
MsG0780037964.01.T01 AT2G46030 63.559 118 29 2 15 132 88 191 4.08e-46 150
MsG0780037964.01.T01 AT5G41340 79.268 82 17 0 52 133 4 85 1.94e-43 140
MsG0780037964.01.T01 AT1G16890 35.955 89 55 2 45 133 4 90 5.73e-16 69.3
MsG0780037964.01.T01 AT1G78870 35.955 89 55 2 45 133 4 90 5.79e-16 69.3
MsG0780037964.01.T01 AT1G16890 35.955 89 55 2 45 133 37 123 7.44e-16 69.7
MsG0780037964.01.T01 AT1G78870 35.955 89 55 2 45 133 37 123 9.31e-16 69.7
MsG0780037964.01.T01 AT1G16890 35.955 89 55 2 45 133 46 132 1.11e-15 69.7
MsG0780037964.01.T01 AT1G78870 35.556 90 55 3 45 133 4 91 1.69e-14 65.5
MsG0780037964.01.T01 AT1G78870 36.111 72 45 1 45 116 37 107 1.95e-14 65.1
MsG0780037964.01.T01 AT3G08700 33.696 92 57 3 44 133 31 120 6.56e-13 62.0
MsG0780037964.01.T01 AT3G08700 33.696 92 57 3 44 133 31 120 6.56e-13 62.0
MsG0780037964.01.T01 AT1G14400 29.213 89 61 2 44 132 35 121 7.19e-13 62.0
MsG0780037964.01.T01 AT1G14400 29.213 89 61 2 44 132 35 121 7.19e-13 62.0
MsG0780037964.01.T01 AT5G50870 30.000 90 62 1 43 132 33 121 7.36e-13 62.8
MsG0780037964.01.T01 AT2G02760 29.213 89 61 2 44 132 35 121 7.58e-13 62.0
MsG0780037964.01.T01 AT2G02760 29.213 89 61 2 44 132 35 121 7.58e-13 62.0
MsG0780037964.01.T01 AT2G02760 29.213 89 61 2 44 132 35 121 7.58e-13 62.0
MsG0780037964.01.T01 AT2G02760 29.213 89 61 2 44 132 35 121 7.58e-13 62.0
MsG0780037964.01.T01 AT5G50870 30.000 90 62 1 43 132 33 121 8.39e-13 62.8
MsG0780037964.01.T01 AT5G56150 31.707 82 53 2 45 126 33 111 1.20e-12 60.5
MsG0780037964.01.T01 AT5G25760 29.885 87 60 1 47 133 39 124 5.88e-12 59.7
MsG0780037964.01.T01 AT5G25760 29.885 87 60 1 47 133 39 124 5.88e-12 59.7
MsG0780037964.01.T01 AT2G16740 30.337 89 60 2 45 133 33 119 1.01e-11 58.9

Find 21 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATAGAAGCAGACTTATA+AGG 0.329409 7:-37180740 None:intergenic
ACTCACATTTCATCAACATT+AGG 0.355490 7:-37180785 None:intergenic
GTCCTTCTCAAGAAGGAGTA+TGG 0.371112 7:+37180687 MsG0780037964.01.T01:CDS
TTTGATATTCAGGTCTGGTT+CGG 0.422307 7:+37180930 MsG0780037964.01.T01:intron
CAGACTTGGAGTCCCATGTT+TGG 0.437496 7:+37180973 MsG0780037964.01.T01:CDS
CAAACATGGGACTCCAAGTC+TGG 0.448863 7:-37180972 None:intergenic
CACAATGGATCTGATGCATT+GGG 0.471037 7:-37181176 None:intergenic
TCACAATGGATCTGATGCAT+TGG 0.485086 7:-37181177 None:intergenic
TTAATAAAGCTTACCAAACA+TGG 0.504008 7:-37180986 None:intergenic
AAACTTACAGTGTATAACTT+AGG 0.519681 7:-37180239 None:intergenic
AGGAGTATGGAAGATAAGAG+TGG 0.530972 7:+37180700 MsG0780037964.01.T01:CDS
TTGGGATATATAAGAAAATG+AGG 0.531166 7:-37181158 None:intergenic
TTCCATACTCCTTCTTGAGA+AGG 0.542561 7:-37180689 None:intergenic
CAGGATGATGAGTGATTACA+TGG 0.544614 7:+37180163 MsG0780037964.01.T01:intron
TAGATGTTATTAACCAGACT+TGG 0.565318 7:+37180959 MsG0780037964.01.T01:CDS
ACCGCAGGTCCTTCTCAAGA+AGG 0.579537 7:+37180680 MsG0780037964.01.T01:CDS
CAGTGTATAACTTAGGTCGA+TGG 0.584949 7:-37180232 None:intergenic
ACATTTCATCAACATTAGGA+TGG 0.594673 7:-37180781 None:intergenic
TAATAAAGCTTACCAAACAT+GGG 0.614807 7:-37180985 None:intergenic
TCAAGGAAGCAGCTTCACAA+TGG 0.625636 7:-37181191 None:intergenic
TCCTTCTTGAGAAGGACCTG+CGG 0.675840 7:-37180681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTCTAATGTTTTTGTAAT+TGG + Chr7:37180128-37180147 MsG0780037964.01.T01:intron 15.0%
!!! ATTCTAATGTTTTTGTAATT+GGG + Chr7:37180129-37180148 MsG0780037964.01.T01:intron 15.0%
!!! AAACTTTCATTGTTTTCTAA+GGG + Chr7:37180822-37180841 MsG0780037964.01.T01:intron 20.0%
!!! AATCTTTTTCTTTGATATTC+AGG + Chr7:37180920-37180939 MsG0780037964.01.T01:intron 20.0%
!!! TTTTAAGATGAAAGTTCTAT+TGG + Chr7:37180857-37180876 MsG0780037964.01.T01:intron 20.0%
! AAACTTACAGTGTATAACTT+AGG - Chr7:37180242-37180261 None:intergenic 25.0%
! AACCTTTACCTTACATATAA+TGG + Chr7:37181010-37181029 MsG0780037964.01.T01:intron 25.0%
! ATTCATTGATGCAACAAAAA+AGG - Chr7:37180295-37180314 None:intergenic 25.0%
! CAAAAACATTAGAATTTCTG+AGG - Chr7:37180124-37180143 None:intergenic 25.0%
! TAATAAAGCTTACCAAACAT+GGG - Chr7:37180988-37181007 None:intergenic 25.0%
! TTAATAAAGCTTACCAAACA+TGG - Chr7:37180989-37181008 None:intergenic 25.0%
! TTCATTGATGCAACAAAAAA+GGG - Chr7:37180294-37180313 None:intergenic 25.0%
! TTGGGATATATAAGAAAATG+AGG - Chr7:37181161-37181180 None:intergenic 25.0%
!! CAAACTTTCATTGTTTTCTA+AGG + Chr7:37180821-37180840 MsG0780037964.01.T01:intron 25.0%
!!! GTTTTTCACTCACTTTTATT+TGG + Chr7:37180653-37180672 MsG0780037964.01.T01:CDS 25.0%
ACAATAGAAGCAGACTTATA+AGG - Chr7:37180743-37180762 None:intergenic 30.0%
ACATTCAATTATGTCTTGTC+AGG + Chr7:37180535-37180554 MsG0780037964.01.T01:intron 30.0%
ACATTTCATCAACATTAGGA+TGG - Chr7:37180784-37180803 None:intergenic 30.0%
ACCACAAATACAATCAAACA+AGG - Chr7:37181083-37181102 None:intergenic 30.0%
ACTCACATTTCATCAACATT+AGG - Chr7:37180788-37180807 None:intergenic 30.0%
CACCATTATATGTAAGGTAA+AGG - Chr7:37181015-37181034 None:intergenic 30.0%
CATTACCACCATTATATGTA+AGG - Chr7:37181021-37181040 None:intergenic 30.0%
CTTTACCTTACATATAATGG+TGG + Chr7:37181013-37181032 MsG0780037964.01.T01:intron 30.0%
GCATCAACACTTCTAATTAA+AGG + Chr7:37180468-37180487 MsG0780037964.01.T01:intron 30.0%
TAGATGTTATTAACCAGACT+TGG + Chr7:37180959-37180978 MsG0780037964.01.T01:CDS 30.0%
TCATTGATGCAACAAAAAAG+GGG - Chr7:37180293-37180312 None:intergenic 30.0%
TTCAACAAAAAACTGTTGCA+TGG - Chr7:37180210-37180229 None:intergenic 30.0%
TTGAATGTAATCACATGTGT+CGG - Chr7:37180523-37180542 None:intergenic 30.0%
! ACCTTGTTTGATTGTATTTG+TGG + Chr7:37181079-37181098 MsG0780037964.01.T01:intron 30.0%
! TTTTCTTTGATATTCAGGTC+TGG + Chr7:37180925-37180944 MsG0780037964.01.T01:intron 30.0%
AACGAGGATGAATTGCATTA+GGG + Chr7:37180332-37180351 MsG0780037964.01.T01:intron 35.0%
AGTCTACTCTTCAGTAAACA+TGG + Chr7:37180062-37180081 MsG0780037964.01.T01:intron 35.0%
AGTGAAAAACCTATGTAGCA+CGG - Chr7:37180643-37180662 None:intergenic 35.0%
CATTGATGCAACAAAAAAGG+GGG - Chr7:37180292-37180311 None:intergenic 35.0%
CGAATTGTGTTTAGCACATA+AGG + Chr7:37180415-37180434 MsG0780037964.01.T01:intron 35.0%
GGGAAAAAATTGATACAACG+GGG - Chr7:37180272-37180291 None:intergenic 35.0%
GGGGAAAAAATTGATACAAC+GGG - Chr7:37180273-37180292 None:intergenic 35.0%
GGGGGAAAAAATTGATACAA+CGG - Chr7:37180274-37180293 None:intergenic 35.0%
TCATCCTCGTTGAAGAAAAT+AGG - Chr7:37180323-37180342 None:intergenic 35.0%
TTTGATATTCAGGTCTGGTT+CGG + Chr7:37180930-37180949 MsG0780037964.01.T01:intron 35.0%
! TAATTGGGTTGTTGTGAATC+AGG + Chr7:37180144-37180163 MsG0780037964.01.T01:intron 35.0%
! TTTGCCTATTTTCTTCAACG+AGG + Chr7:37180316-37180335 MsG0780037964.01.T01:intron 35.0%
!! TAGAAGTGTTGATGCTATAC+AGG - Chr7:37180463-37180482 None:intergenic 35.0%
ATTGCATTAGGGTCTATGAC+TGG + Chr7:37180343-37180362 MsG0780037964.01.T01:intron 40.0%
CAACGAGGATGAATTGCATT+AGG + Chr7:37180331-37180350 MsG0780037964.01.T01:intron 40.0%
CACAATGGATCTGATGCATT+GGG - Chr7:37181179-37181198 None:intergenic 40.0%
CAGGATGATGAGTGATTACA+TGG + Chr7:37180163-37180182 MsG0780037964.01.T01:intron 40.0%
CAGTGTATAACTTAGGTCGA+TGG - Chr7:37180235-37180254 None:intergenic 40.0%
TCACAATGGATCTGATGCAT+TGG - Chr7:37181180-37181199 None:intergenic 40.0%
TTCCATACTCCTTCTTGAGA+AGG - Chr7:37180692-37180711 None:intergenic 40.0%
! AGGAGTATGGAAGATAAGAG+TGG + Chr7:37180700-37180719 MsG0780037964.01.T01:CDS 40.0%
! CTTCTAATTAAAGGCGTGTC+CGG + Chr7:37180477-37180496 MsG0780037964.01.T01:intron 40.0%
! GTCTATGACTGGTTAACTCT+AGG + Chr7:37180354-37180373 MsG0780037964.01.T01:intron 40.0%
! TTAAAGGCATGTCCAATGTC+CGG - Chr7:37180610-37180629 None:intergenic 40.0%
!! TGTTTTCTAAGGGACATCGA+AGG + Chr7:37180832-37180851 MsG0780037964.01.T01:intron 40.0%
GCACGGACTCTTCAGATTAA+AGG - Chr7:37180626-37180645 None:intergenic 45.0%
GTCCTTCTCAAGAAGGAGTA+TGG + Chr7:37180687-37180706 MsG0780037964.01.T01:CDS 45.0%
!! CTTTTATTTGGACTCACCGC+AGG + Chr7:37180665-37180684 MsG0780037964.01.T01:CDS 45.0%
CAAACATGGGACTCCAAGTC+TGG - Chr7:37180975-37180994 None:intergenic 50.0%
CAACACGACACTGACACACA+TGG - Chr7:37180584-37180603 None:intergenic 50.0%
CAGACTTGGAGTCCCATGTT+TGG + Chr7:37180973-37180992 MsG0780037964.01.T01:CDS 50.0%
CATGTGTGTCAGTGTCGTGT+TGG + Chr7:37180582-37180601 MsG0780037964.01.T01:intron 50.0%
CTGAAGAGTCCGTGCTACAT+AGG + Chr7:37180631-37180650 MsG0780037964.01.T01:intron 50.0%
TCCTTCTTGAGAAGGACCTG+CGG - Chr7:37180684-37180703 None:intergenic 50.0%
ACCGCAGGTCCTTCTCAAGA+AGG + Chr7:37180680-37180699 MsG0780037964.01.T01:CDS 55.0%
! GTCGTGTTGGTGTAGGACAC+CGG - Chr7:37180499-37180518 None:intergenic 55.0%
! TGTCAGTGTCGTGTTGGTGC+CGG + Chr7:37180588-37180607 MsG0780037964.01.T01:intron 55.0%
!! CACATGTGTCGGTGTCGTGT+TGG - Chr7:37180512-37180531 None:intergenic 55.0%
!! TGTCGGTGTCGTGTTGGTGT+AGG - Chr7:37180506-37180525 None:intergenic 55.0%
!! GTCGTGTTGGTGCCGGACAT+TGG + Chr7:37180595-37180614 MsG0780037964.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 37180034 37181199 37180034 ID=MsG0780037964.01;Name=MsG0780037964.01
Chr7 mRNA 37180034 37181199 37180034 ID=MsG0780037964.01.T01;Parent=MsG0780037964.01;Name=MsG0780037964.01.T01;_AED=0.32;_eAED=0.33;_QI=0|0.71|0.62|1|0.71|0.62|8|0|133
Chr7 exon 37181129 37181199 37181129 ID=MsG0780037964.01.T01:exon:5746;Parent=MsG0780037964.01.T01
Chr7 exon 37180942 37180994 37180942 ID=MsG0780037964.01.T01:exon:5745;Parent=MsG0780037964.01.T01
Chr7 exon 37180653 37180801 37180653 ID=MsG0780037964.01.T01:exon:5744;Parent=MsG0780037964.01.T01
Chr7 exon 37180166 37180253 37180166 ID=MsG0780037964.01.T01:exon:5743;Parent=MsG0780037964.01.T01
Chr7 exon 37180034 37180074 37180034 ID=MsG0780037964.01.T01:exon:5742;Parent=MsG0780037964.01.T01
Chr7 CDS 37180034 37180074 37180034 ID=MsG0780037964.01.T01:cds;Parent=MsG0780037964.01.T01
Chr7 CDS 37180166 37180253 37180166 ID=MsG0780037964.01.T01:cds;Parent=MsG0780037964.01.T01
Chr7 CDS 37180653 37180801 37180653 ID=MsG0780037964.01.T01:cds;Parent=MsG0780037964.01.T01
Chr7 CDS 37180942 37180994 37180942 ID=MsG0780037964.01.T01:cds;Parent=MsG0780037964.01.T01
Chr7 CDS 37181129 37181199 37181129 ID=MsG0780037964.01.T01:cds;Parent=MsG0780037964.01.T01
Gene Sequence

>MsG0780037964.01.T01

ATGGGTAATATTGTAATTAGCGTTCTTAAGTCTACTCTTCAGATGATGAGTGATTACATGGTGAAGAAGATGATCAATGATGCCATGCAACAGTTTTTTGTTGAATTCCATCGACCTAAGTTATACACTGTTTTTCACTCACTTTTATTTGGACTCACCGCAGGTCCTTCTCAAGAAGGAGTATGGAAGATAAGAGTGGAGCTACATGATGCTTATCCTTATAAGTCTGCTTCTATTGTTTTTGTCAACAAAATTTACCATCCTAATGTTGATGAAATGTCTGGTTCGGTTTGTTTAGATGTTATTAACCAGACTTGGAGTCCCATGTTTGATCTTGTTAATGTTTTTGAAGTGTTTCTTCCTCATTTTCTTATATATCCCAATGCATCAGATCCATTGTGA

Protein sequence

>MsG0780037964.01.T01

MGNIVISVLKSTLQMMSDYMVKKMINDAMQQFFVEFHRPKLYTVFHSLLFGLTAGPSQEGVWKIRVELHDAYPYKSASIVFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPHFLIYPNASDPL*