AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020832.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020832.01.T01 MTR_4g060620 96.809 94 3 0 1 94 1 94 7.50e-64 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020832.01.T01 AT5G60400 40.449 89 51 1 6 94 5 91 1.51e-11 55.8

Find 17 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTCCTCTCTGTCTTTC+TGG 0.075036 4:-49317957 MsG0480020832.01.T01:CDS
ACTTACTAGAATGTTAATTT+AGG 0.250075 4:+49317462 None:intergenic
GTTTAACATAGACTGCATTT+TGG 0.292624 4:+49317995 None:intergenic
AAAAGCTGCTTAGTTAGATT+TGG 0.304540 4:-49317595 MsG0480020832.01.T01:exon
TGATGATGATCTTGAAGATC+TGG 0.442771 4:-49317667 MsG0480020832.01.T01:CDS
AGTGCATGCAAATCAAGAAA+AGG 0.472454 4:-49317733 MsG0480020832.01.T01:CDS
CTGTCTCTTACTTATCTCCC+AGG 0.495221 4:-49317627 MsG0480020832.01.T01:CDS
TTAAACCTTCGTGCTACTCA+TGG 0.498726 4:-49317978 MsG0480020832.01.T01:CDS
ACCGCAGAAGAAAAGATATG+AGG 0.523865 4:-49317767 MsG0480020832.01.T01:CDS
GTGCATGCAAATCAAGAAAA+GGG 0.524986 4:-49317732 MsG0480020832.01.T01:CDS
TACTCTTTGGAAAGAGCAAG+AGG 0.575505 4:+49317924 None:intergenic
AACATTCTAGTAAGTTCAGA+CGG 0.600784 4:-49317454 None:intergenic
TTTGGAAAGAGCAAGAGGGT+TGG 0.601098 4:+49317929 None:intergenic
AAAAGGATGGGAAATAAAGG+AGG 0.624688 4:+49318046 None:intergenic
ACTCTTTGGAAAGAGCAAGA+GGG 0.667434 4:+49317925 None:intergenic
GTGATTCCAGAAAGACAGAG+AGG 0.712435 4:+49317951 None:intergenic
GAACTCCATGAGTAGCACGA+AGG 0.732005 4:+49317973 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTAATTTACTCTT+TGG + Chr4:49317655-49317674 None:intergenic 10.0%
!! ACTTACTAGAATGTTAATTT+AGG + Chr4:49318104-49318123 None:intergenic 20.0%
!! TGATATATGTTTGAATTTGA+TGG - Chr4:49317754-49317773 MsG0480020832.01.T01:CDS 20.0%
!! TCAAAGAAACAATGATTTTG+TGG - Chr4:49317863-49317882 MsG0480020832.01.T01:intron 25.0%
!! TCTAAATCACTTTCATTTTC+TGG - Chr4:49318005-49318024 MsG0480020832.01.T01:exon 25.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + Chr4:49317537-49317556 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + Chr4:49317545-49317564 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + Chr4:49317551-49317570 None:intergenic 25.0%
AAAAGCTGCTTAGTTAGATT+TGG - Chr4:49317968-49317987 MsG0480020832.01.T01:CDS 30.0%
GAAAAAAGGATGGGAAATAA+AGG + Chr4:49317523-49317542 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + Chr4:49317532-49317551 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - Chr4:49317873-49317892 MsG0480020832.01.T01:intron 30.0%
! GTTTAACATAGACTGCATTT+TGG + Chr4:49317571-49317590 None:intergenic 30.0%
!! ATGTTTGAATTTGATGGTGT+AGG - Chr4:49317760-49317779 MsG0480020832.01.T01:CDS 30.0%
AAAAGGATGGGAAATAAAGG+AGG + Chr4:49317520-49317539 None:intergenic 35.0%
AGTGCATGCAAATCAAGAAA+AGG - Chr4:49317830-49317849 MsG0480020832.01.T01:intron 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + Chr4:49317533-49317552 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + Chr4:49317697-49317716 None:intergenic 35.0%
GTGCATGCAAATCAAGAAAA+GGG - Chr4:49317831-49317850 MsG0480020832.01.T01:intron 35.0%
! TCCTCATATCTTTTCTTCTG+CGG + Chr4:49317800-49317819 None:intergenic 35.0%
! TGATGATGATCTTGAAGATC+TGG - Chr4:49317896-49317915 MsG0480020832.01.T01:intron 35.0%
!! TTGAATTTGATGGTGTAGGA+TGG - Chr4:49317764-49317783 MsG0480020832.01.T01:CDS 35.0%
ACCGCAGAAGAAAAGATATG+AGG - Chr4:49317796-49317815 MsG0480020832.01.T01:intron 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + Chr4:49317641-49317660 None:intergenic 40.0%
! CAGCTTTTGCTGATAAACCT+GGG + Chr4:49317956-49317975 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + Chr4:49317642-49317661 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - Chr4:49317585-49317604 MsG0480020832.01.T01:three_prime_UTR 40.0%
CTGTCTCTTACTTATCTCCC+AGG - Chr4:49317936-49317955 MsG0480020832.01.T01:CDS 45.0%
GTGATTCCAGAAAGACAGAG+AGG + Chr4:49317615-49317634 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + Chr4:49317637-49317656 None:intergenic 45.0%
! GCAGCTTTTGCTGATAAACC+TGG + Chr4:49317957-49317976 None:intergenic 45.0%
GAACTCCATGAGTAGCACGA+AGG + Chr4:49317593-49317612 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - Chr4:49317606-49317625 MsG0480020832.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 49317455 49318130 49317455 ID=MsG0480020832.01;Name=MsG0480020832.01
Chr4 mRNA 49317455 49318130 49317455 ID=MsG0480020832.01.T01;Parent=MsG0480020832.01;Name=MsG0480020832.01.T01;_AED=0.25;_eAED=0.25;_QI=118|1|1|1|1|1|2|150|94
Chr4 exon 49317455 49317803 49317455 ID=MsG0480020832.01.T01:exon:745;Parent=MsG0480020832.01.T01
Chr4 exon 49317927 49318130 49317927 ID=MsG0480020832.01.T01:exon:744;Parent=MsG0480020832.01.T01
Chr4 five_prime_UTR 49318013 49318130 49318013 ID=MsG0480020832.01.T01:five_prime_utr;Parent=MsG0480020832.01.T01
Chr4 CDS 49317927 49318012 49317927 ID=MsG0480020832.01.T01:cds;Parent=MsG0480020832.01.T01
Chr4 CDS 49317605 49317803 49317605 ID=MsG0480020832.01.T01:cds;Parent=MsG0480020832.01.T01
Chr4 three_prime_UTR 49317455 49317604 49317455 ID=MsG0480020832.01.T01:three_prime_utr;Parent=MsG0480020832.01.T01
Gene Sequence

>MsG0480020832.01.T01

ATGCAGTCTATGTTAAACCTTCGTGCTACTCATGGAGTTCCTCTCTGTCTTTCTGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGATGGCAGATCATGACCGCAGAAGAAAAGATATGAGGAAGCAAAGATCAGTGCATGCAAATCAAGAAAAGGGTGCACAAAGTCAAAGAAACAATGATTTTGTGGAGATGAAAGGTGATGATGATCTTGAAGATCTGGATGAAATCTTGCATCAACTGTCTCTTACTTATCTCCCAGGTTTATCAGCAAAAGCTGCTTAG

Protein sequence

>MsG0480020832.01.T01

MQSMLNLRATHGVPLCLSGITNPLALSKEMADHDRRRKDMRKQRSVHANQEKGAQSQRNNDFVEMKGDDDLEDLDEILHQLSLTYLPGLSAKAA*