Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041967.01.T01 | KAF5961329.1 | 96.429 | 84 | 1 | 1 | 1 | 84 | 72 | 153 | 6.21E-51 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041967.01.T01 | Q9XHE4 | 97.436 | 78 | 2 | 0 | 7 | 84 | 15 | 92 | 5.61E-50 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041967.01.T01 | A0A7J7I8S3 | 96.429 | 84 | 1 | 1 | 1 | 84 | 72 | 153 | 2.96e-51 | 167 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048135.01 | MsG0880041967.01 | 0.810066 | 1.386284e-50 | 8.275361e-48 |
| MsG0080048687.01 | MsG0880041967.01 | 0.800731 | 1.255856e-48 | 5.891225e-46 |
| MsG0080048971.01 | MsG0880041967.01 | 0.860499 | 1.979828e-63 | 5.423046e-60 |
| MsG0180000575.01 | MsG0880041967.01 | 0.886290 | 3.890042e-72 | 2.761767e-68 |
| MsG0180000614.01 | MsG0880041967.01 | 0.832754 | 7.891127e-56 | 8.903525e-53 |
| MsG0180000615.01 | MsG0880041967.01 | 0.841586 | 4.345001e-58 | 6.413886e-55 |
| MsG0180000743.01 | MsG0880041967.01 | 0.824165 | 9.358367e-54 | 8.224985e-51 |
| MsG0180003313.01 | MsG0880041967.01 | 0.820569 | 6.400403e-53 | 5.082472e-50 |
| MsG0180003612.01 | MsG0880041967.01 | 0.850147 | 2.048712e-60 | 3.974973e-57 |
| MsG0180003729.01 | MsG0880041967.01 | 0.805959 | 1.037520e-49 | 5.563127e-47 |
| MsG0180003793.01 | MsG0880041967.01 | 0.841490 | 4.604829e-58 | 6.777315e-55 |
| MsG0180004129.01 | MsG0880041967.01 | 0.838903 | 2.180912e-57 | 2.962615e-54 |
| MsG0180004130.01 | MsG0880041967.01 | 0.817849 | 2.664549e-52 | 1.962458e-49 |
| MsG0180004509.01 | MsG0880041967.01 | 0.832398 | 9.672359e-56 | 1.079826e-52 |
| MsG0180004625.01 | MsG0880041967.01 | 0.805996 | 1.019330e-49 | 5.470861e-47 |
| MsG0180004854.01 | MsG0880041967.01 | 0.816949 | 4.247191e-52 | 3.052168e-49 |
| MsG0180005366.01 | MsG0880041967.01 | 0.855620 | 5.584463e-62 | 1.297003e-58 |
| MsG0180005426.01 | MsG0880041967.01 | 0.864689 | 1.014890e-64 | 3.214871e-61 |
| MsG0180005659.01 | MsG0880041967.01 | 0.828051 | 1.114209e-54 | 1.095089e-51 |
| MsG0280006424.01 | MsG0880041967.01 | 0.859820 | 3.174618e-63 | 8.495482e-60 |
| MsG0780040782.01 | MsG0880041967.01 | 0.905920 | 2.576707e-80 | 4.264470e-76 |
| MsG0780040900.01 | MsG0880041967.01 | 0.891790 | 2.879172e-74 | 2.561193e-70 |
| MsG0780041048.01 | MsG0880041967.01 | 0.809277 | 2.049137e-50 | 1.197963e-47 |
| MsG0780041147.01 | MsG0880041967.01 | 0.862795 | 3.935087e-64 | 1.167024e-60 |
| MsG0780041223.01 | MsG0880041967.01 | 0.846898 | 1.626311e-59 | 2.840829e-56 |
| MsG0780041224.01 | MsG0880041967.01 | 0.868812 | 4.941419e-66 | 1.812015e-62 |
| MsG0780041462.01 | MsG0880041967.01 | 0.808147 | 3.573407e-50 | 2.027629e-47 |
| MsG0780041519.01 | MsG0880041967.01 | 0.874145 | 8.488659e-68 | 3.779265e-64 |
| MsG0880041967.01 | MsG0880042125.01 | 0.858186 | 9.789294e-63 | 2.477415e-59 |
| MsG0880041967.01 | MsG0880042131.01 | 0.821874 | 3.201711e-53 | 2.637099e-50 |
| MsG0880041967.01 | MsG0880043517.01 | 0.800221 | 1.595408e-48 | 7.387836e-46 |
| MsG0880041967.01 | MsG0880043770.01 | 0.801267 | 9.762254e-49 | 4.641977e-46 |
| MsG0880041967.01 | MsG0880044711.01 | 0.848460 | 6.045247e-60 | 1.110262e-56 |
| MsG0880041967.01 | MsG0880046444.01 | 0.878913 | 1.910310e-69 | 1.015229e-65 |
| MsG0880041967.01 | MsG0880046575.01 | 0.803544 | 3.313593e-49 | 1.669789e-46 |
| MsG0880041967.01 | MsG0880046879.01 | 0.863134 | 3.092713e-64 | 9.277081e-61 |
| MsG0280010801.01 | MsG0880041967.01 | 0.831624 | 1.501752e-55 | 1.638746e-52 |
| MsG0280010995.01 | MsG0880041967.01 | 0.858654 | 7.103719e-63 | 1.826141e-59 |
| MsG0280011234.01 | MsG0880041967.01 | 0.838292 | 3.136025e-57 | 4.181208e-54 |
| MsG0280011308.01 | MsG0880041967.01 | 0.855588 | 5.703835e-62 | 1.323421e-58 |
| MsG0380012073.01 | MsG0880041967.01 | 0.848059 | 7.800008e-60 | 1.413816e-56 |
| MsG0680031628.01 | MsG0880041967.01 | 0.802201 | 6.275896e-49 | 3.055673e-46 |
| MsG0680031763.01 | MsG0880041967.01 | 0.861195 | 1.216491e-63 | 3.413337e-60 |
| MsG0680032770.01 | MsG0880041967.01 | 0.869063 | 4.098642e-66 | 1.517060e-62 |
| MsG0480018477.01 | MsG0880041967.01 | 0.825061 | 5.754303e-54 | 5.189114e-51 |
| MsG0480020959.01 | MsG0880041967.01 | 0.825630 | 4.220241e-54 | 3.867924e-51 |
| MsG0480021006.01 | MsG0880041967.01 | 0.826095 | 3.273426e-54 | 3.039941e-51 |
| MsG0480021159.01 | MsG0880041967.01 | 0.870476 | 1.417435e-66 | 5.521779e-63 |
| MsG0480022913.01 | MsG0880041967.01 | 0.827123 | 1.861183e-54 | 1.780277e-51 |
| MsG0480023149.01 | MsG0880041967.01 | 0.822645 | 2.120883e-53 | 1.785780e-50 |
| MsG0480023399.01 | MsG0880041967.01 | 0.836565 | 8.684693e-57 | 1.098561e-53 |
| MsG0480023467.01 | MsG0880041967.01 | 0.872945 | 2.153000e-67 | 9.176032e-64 |
| MsG0480023771.01 | MsG0880041967.01 | 0.839750 | 1.314184e-57 | 1.832221e-54 |
| MsG0580024369.01 | MsG0880041967.01 | 0.861480 | 9.961707e-64 | 2.822744e-60 |
| MsG0380016778.01 | MsG0880041967.01 | 0.830658 | 2.594740e-55 | 2.751439e-52 |
| MsG0380017382.01 | MsG0880041967.01 | 0.848649 | 5.359137e-60 | 9.901425e-57 |
| MsG0480018084.01 | MsG0880041967.01 | 0.828943 | 6.786561e-55 | 6.845015e-52 |
| MsG0580027043.01 | MsG0880041967.01 | 0.847897 | 8.647746e-60 | 1.559675e-56 |
| MsG0580029132.01 | MsG0880041967.01 | 0.848005 | 8.073034e-60 | 1.460909e-56 |
| MsG0280006819.01 | MsG0880041967.01 | 0.819017 | 1.448039e-52 | 1.101497e-49 |
| MsG0280006836.01 | MsG0880041967.01 | 0.877870 | 4.439585e-69 | 2.268673e-65 |
| MsG0280006993.01 | MsG0880041967.01 | 0.833827 | 4.263845e-56 | 4.968392e-53 |
| MsG0280007634.01 | MsG0880041967.01 | 0.808176 | 3.522346e-50 | 2.000264e-47 |
| MsG0280008118.01 | MsG0880041967.01 | 0.802684 | 4.992489e-49 | 2.460918e-46 |
| MsG0280009372.01 | MsG0880041967.01 | 0.815286 | 9.995236e-52 | 6.862522e-49 |
| MsG0280009375.01 | MsG0880041967.01 | 0.819806 | 9.572871e-53 | 7.442943e-50 |
| MsG0280010383.01 | MsG0880041967.01 | 0.857544 | 1.518388e-62 | 3.761075e-59 |
| MsG0280010495.01 | MsG0880041967.01 | 0.873301 | 1.634718e-67 | 7.059265e-64 |
| MsG0280010525.01 | MsG0880041967.01 | 0.846018 | 2.827280e-59 | 4.800968e-56 |
| MsG0680035848.01 | MsG0880041967.01 | 0.811258 | 7.660821e-51 | 4.719613e-48 |
| MsG0780036546.01 | MsG0880041967.01 | 0.819401 | 1.183908e-52 | 9.100934e-50 |
| MsG0780036547.01 | MsG0880041967.01 | 0.864177 | 1.467270e-64 | 4.564547e-61 |
| MsG0780036822.01 | MsG0880041967.01 | 0.851573 | 8.127604e-61 | 1.652190e-57 |
| MsG0780038400.01 | MsG0880041967.01 | 0.855625 | 5.565594e-62 | 1.292846e-58 |
| MsG0780038587.01 | MsG0880041967.01 | 0.824540 | 7.637439e-54 | 6.784975e-51 |
| MsG0780039848.01 | MsG0880041967.01 | 0.847670 | 9.984769e-60 | 1.788008e-56 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041967.01.T01 | MTR_4g099160 | 100.000 | 78 | 0 | 0 | 7 | 84 | 15 | 92 | 1.05e-52 | 159 |
| MsG0880041967.01.T01 | MTR_4g103340 | 100.000 | 78 | 0 | 0 | 7 | 84 | 15 | 92 | 1.05e-52 | 159 |
| MsG0880041967.01.T01 | MTR_8g007485 | 100.000 | 78 | 0 | 0 | 7 | 84 | 15 | 92 | 1.05e-52 | 159 |
| MsG0880041967.01.T01 | MTR_0199s0040 | 100.000 | 78 | 0 | 0 | 7 | 84 | 96 | 173 | 3.17e-52 | 160 |
| MsG0880041967.01.T01 | MTR_4g103340 | 98.276 | 58 | 1 | 0 | 7 | 64 | 15 | 72 | 6.31e-37 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041967.01.T01 | AT3G10950 | 93.590 | 78 | 5 | 0 | 7 | 84 | 15 | 92 | 1.77e-50 | 154 |
| MsG0880041967.01.T01 | AT3G60245 | 93.506 | 77 | 5 | 0 | 7 | 83 | 15 | 91 | 2.09e-49 | 151 |
Find 29 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTTGTGCTTTAAAATTTC+AGG | 0.190246 | 8:+1930838 | None:intergenic |
| AGAGGAAGGCTGTCGGTATT+TGG | 0.231684 | 8:+1931047 | MsG0880041967.01.T01:CDS |
| GAGGAAGGCTGTCGGTATTT+GGG | 0.275108 | 8:+1931048 | MsG0880041967.01.T01:CDS |
| AAAATTTCAGGCACCCGATA+TGG | 0.306749 | 8:+1930850 | MsG0880041967.01.T01:CDS |
| TCCTCAAACTAGCACCATAT+CGG | 0.361347 | 8:-1930864 | None:intergenic |
| CTATATGTTGTCTAGGGTTA+AGG | 0.365835 | 8:+1931527 | MsG0880041967.01.T01:three_prime_UTR |
| ATCAAATATAATCTTAAAAC+AGG | 0.400478 | 8:-1931337 | None:intergenic |
| GAGGAAGCAAATTAAGAAGA+TGG | 0.413539 | 8:+1930882 | MsG0880041967.01.T01:CDS |
| CCCGATATGGTGCTAGTTTG+AGG | 0.444329 | 8:+1930863 | MsG0880041967.01.T01:CDS |
| CAGTCTGCTCCCTCAACCTT+CGG | 0.446744 | 8:-1931250 | None:intergenic |
| TAATACAGTATGCTGTTAAG+AGG | 0.458691 | 8:+1931029 | MsG0880041967.01.T01:intron |
| TGATTTGCTATATGTTGTCT+AGG | 0.463453 | 8:+1931520 | MsG0880041967.01.T01:three_prime_UTR |
| CCTCAAACTAGCACCATATC+GGG | 0.503731 | 8:-1930863 | None:intergenic |
| GAAGCACCATCCGAAGGTTG+AGG | 0.512356 | 8:+1931240 | MsG0880041967.01.T01:CDS |
| ATTTGGGGCTGCAAAGATTG+TGG | 0.525352 | 8:+1931064 | MsG0880041967.01.T01:CDS |
| GATTTGCTATATGTTGTCTA+GGG | 0.527970 | 8:+1931521 | MsG0880041967.01.T01:three_prime_UTR |
| AAGCACCATCCGAAGGTTGA+GGG | 0.541427 | 8:+1931241 | MsG0880041967.01.T01:CDS |
| AGGAAGGCTGTCGGTATTTG+GGG | 0.543829 | 8:+1931049 | MsG0880041967.01.T01:CDS |
| CTGCTCCCTCAACCTTCGGA+TGG | 0.565963 | 8:-1931246 | None:intergenic |
| GATTGTGGAAAAGTGAAAGC+TGG | 0.576075 | 8:+1931079 | MsG0880041967.01.T01:CDS |
| AAATTCTTCTGCGAGTTCTG+TGG | 0.586791 | 8:+1930922 | MsG0880041967.01.T01:CDS |
| ACAGTATGCTGTTAAGAGGA+AGG | 0.590694 | 8:+1931033 | MsG0880041967.01.T01:intron |
| GCTTCTCACAGTTACAGCAC+TGG | 0.604667 | 8:-1931222 | None:intergenic |
| CTTCTGCGAGTTCTGTGGAA+AGG | 0.605984 | 8:+1930927 | MsG0880041967.01.T01:CDS |
| CTGTGAGAAGCACCATCCGA+AGG | 0.653930 | 8:+1931234 | MsG0880041967.01.T01:CDS |
| GCTGTTAAGAGGAAGGCTGT+CGG | 0.664645 | 8:+1931040 | MsG0880041967.01.T01:CDS |
| TCAAATGCTATCTAGTTACG+TGG | 0.670577 | 8:+1931429 | MsG0880041967.01.T01:three_prime_UTR |
| ACTGATTCATCATCGTACGA+AGG | 0.681141 | 8:-1931456 | None:intergenic |
| TGTGGAAAAGTGAAAGCTGG+CGG | 0.773884 | 8:+1931082 | MsG0880041967.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAGATTATATTTGATT+TGG | + | Chr8:1931341-1931360 | MsG0880041967.01.T01:three_prime_UTR | 10.0% |
| !! | ATCAAATATAATCTTAAAAC+AGG | - | Chr8:1931340-1931359 | None:intergenic | 15.0% |
| !!! | GTTTTTTTTTTTTTGCTAAT+AGG | + | Chr8:1931477-1931496 | MsG0880041967.01.T01:three_prime_UTR | 15.0% |
| ! | ATAGACAAGAAAATAAACCA+AGG | - | Chr8:1931152-1931171 | None:intergenic | 25.0% |
| ! | CAAAACGACAAAAATGTAAA+TGG | - | Chr8:1931287-1931306 | None:intergenic | 25.0% |
| ! | TAGACAAGAAAATAAACCAA+GGG | - | Chr8:1931151-1931170 | None:intergenic | 25.0% |
| !!! | CATTTTTGTCGTTTTGTTTT+TGG | + | Chr8:1931291-1931310 | MsG0880041967.01.T01:three_prime_UTR | 25.0% |
| AGACAAGAAAATAAACCAAG+GGG | - | Chr8:1931150-1931169 | None:intergenic | 30.0% | |
| TAATACAGTATGCTGTTAAG+AGG | + | Chr8:1931029-1931048 | MsG0880041967.01.T01:intron | 30.0% | |
| !! | GATTTGCTATATGTTGTCTA+GGG | + | Chr8:1931521-1931540 | MsG0880041967.01.T01:three_prime_UTR | 30.0% |
| !! | TGATTTGCTATATGTTGTCT+AGG | + | Chr8:1931520-1931539 | MsG0880041967.01.T01:three_prime_UTR | 30.0% |
| AATACACAAGAACAGAAGCA+CGG | - | Chr8:1931186-1931205 | None:intergenic | 35.0% | |
| GACAAGAAAATAAACCAAGG+GGG | - | Chr8:1931149-1931168 | None:intergenic | 35.0% | |
| GAGGAAGCAAATTAAGAAGA+TGG | + | Chr8:1930882-1930901 | MsG0880041967.01.T01:CDS | 35.0% | |
| TCAAATGCTATCTAGTTACG+TGG | + | Chr8:1931429-1931448 | MsG0880041967.01.T01:three_prime_UTR | 35.0% | |
| ! | CTATATGTTGTCTAGGGTTA+AGG | + | Chr8:1931527-1931546 | MsG0880041967.01.T01:three_prime_UTR | 35.0% |
| ! | TGATGAACAAGAAGTTTTGC+TGG | + | Chr8:1931369-1931388 | MsG0880041967.01.T01:three_prime_UTR | 35.0% |
| ! | TTCAAATCTATTTTCCCCCT+TGG | + | Chr8:1931132-1931151 | MsG0880041967.01.T01:intron | 35.0% |
| AAAATTTCAGGCACCCGATA+TGG | + | Chr8:1930850-1930869 | MsG0880041967.01.T01:CDS | 40.0% | |
| AAATTCTTCTGCGAGTTCTG+TGG | + | Chr8:1930922-1930941 | MsG0880041967.01.T01:CDS | 40.0% | |
| ACAGTATGCTGTTAAGAGGA+AGG | + | Chr8:1931033-1931052 | MsG0880041967.01.T01:intron | 40.0% | |
| GATTGAATTCATCGTCGTAG+AGG | - | Chr8:1930964-1930983 | None:intergenic | 40.0% | |
| GATTGTGGAAAAGTGAAAGC+TGG | + | Chr8:1931079-1931098 | MsG0880041967.01.T01:CDS | 40.0% | |
| GTATTAACAACATCGCGTGA+CGG | - | Chr8:1931015-1931034 | None:intergenic | 40.0% | |
| TCCTCAAACTAGCACCATAT+CGG | - | Chr8:1930867-1930886 | None:intergenic | 40.0% | |
| ! | ACTGATTCATCATCGTACGA+AGG | - | Chr8:1931459-1931478 | None:intergenic | 40.0% |
| CCTCAAACTAGCACCATATC+GGG | - | Chr8:1930866-1930885 | None:intergenic | 45.0% | |
| TGTGGAAAAGTGAAAGCTGG+CGG | + | Chr8:1931082-1931101 | MsG0880041967.01.T01:CDS | 45.0% | |
| ! | ATTTGGGGCTGCAAAGATTG+TGG | + | Chr8:1931064-1931083 | MsG0880041967.01.T01:CDS | 45.0% |
| AAGCACCATCCGAAGGTTGA+GGG | + | Chr8:1931241-1931260 | MsG0880041967.01.T01:CDS | 50.0% | |
| AGAGGAAGGCTGTCGGTATT+TGG | + | Chr8:1931047-1931066 | MsG0880041967.01.T01:CDS | 50.0% | |
| AGGAAGGCTGTCGGTATTTG+GGG | + | Chr8:1931049-1931068 | MsG0880041967.01.T01:CDS | 50.0% | |
| CTTCTGCGAGTTCTGTGGAA+AGG | + | Chr8:1930927-1930946 | MsG0880041967.01.T01:CDS | 50.0% | |
| GAGGAAGGCTGTCGGTATTT+GGG | + | Chr8:1931048-1931067 | MsG0880041967.01.T01:CDS | 50.0% | |
| GCTGTTAAGAGGAAGGCTGT+CGG | + | Chr8:1931040-1931059 | MsG0880041967.01.T01:CDS | 50.0% | |
| GCTTCTCACAGTTACAGCAC+TGG | - | Chr8:1931225-1931244 | None:intergenic | 50.0% | |
| ! | CCCGATATGGTGCTAGTTTG+AGG | + | Chr8:1930863-1930882 | MsG0880041967.01.T01:CDS | 50.0% |
| CAGTCTGCTCCCTCAACCTT+CGG | - | Chr8:1931253-1931272 | None:intergenic | 55.0% | |
| ! | GAAGCACCATCCGAAGGTTG+AGG | + | Chr8:1931240-1931259 | MsG0880041967.01.T01:CDS | 55.0% |
| !! | CTGTGAGAAGCACCATCCGA+AGG | + | Chr8:1931234-1931253 | MsG0880041967.01.T01:CDS | 55.0% |
| CTGCTCCCTCAACCTTCGGA+TGG | - | Chr8:1931249-1931268 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 1930841 | 1931571 | 1930841 | ID=MsG0880041967.01;Name=MsG0880041967.01 |
| Chr8 | mRNA | 1930841 | 1931571 | 1930841 | ID=MsG0880041967.01.T01;Parent=MsG0880041967.01;Name=MsG0880041967.01.T01;_AED=0.46;_eAED=0.48;_QI=0|1|0.66|1|1|1|3|291|84 |
| Chr8 | exon | 1930841 | 1930948 | 1930841 | ID=MsG0880041967.01.T01:exon:1184;Parent=MsG0880041967.01.T01 |
| Chr8 | exon | 1931037 | 1931119 | 1931037 | ID=MsG0880041967.01.T01:exon:1185;Parent=MsG0880041967.01.T01 |
| Chr8 | exon | 1931217 | 1931571 | 1931217 | ID=MsG0880041967.01.T01:exon:1186;Parent=MsG0880041967.01.T01 |
| Chr8 | CDS | 1930841 | 1930948 | 1930841 | ID=MsG0880041967.01.T01:cds;Parent=MsG0880041967.01.T01 |
| Chr8 | CDS | 1931037 | 1931119 | 1931037 | ID=MsG0880041967.01.T01:cds;Parent=MsG0880041967.01.T01 |
| Chr8 | CDS | 1931217 | 1931280 | 1931217 | ID=MsG0880041967.01.T01:cds;Parent=MsG0880041967.01.T01 |
| Chr8 | three_prime_UTR | 1931281 | 1931571 | 1931281 | ID=MsG0880041967.01.T01:three_prime_utr;Parent=MsG0880041967.01.T01 |
| Gene Sequence |
| Protein sequence |
