AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044881.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044881.01.T01 MTR_4g097175 100.000 136 0 0 1 136 1 136 1.38e-96 274
MsG0880044881.01.T01 MTR_1g023630 100.000 136 0 0 1 136 1 136 1.38e-96 274
MsG0880044881.01.T01 MTR_8g061940 100.000 136 0 0 1 136 1 136 1.38e-96 274
MsG0880044881.01.T01 MTR_8g099430 99.265 136 1 0 1 136 1 136 5.05e-96 272
MsG0880044881.01.T01 MTR_4g097170 99.265 136 1 0 1 136 42 177 8.03e-95 271
MsG0880044881.01.T01 MTR_7g059070 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_5g029820 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_5g029770 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_4g065990 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_4g088150 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_2g035230 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_8g092720 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_8g063500 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_8g103245 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_8g092820 97.059 136 4 0 1 136 1 136 3.42e-93 265
MsG0880044881.01.T01 MTR_7g013610 97.059 136 4 0 1 136 100 235 2.74e-92 266
MsG0880044881.01.T01 MTR_2g082310 92.647 136 10 0 1 136 1 136 4.34e-90 257
MsG0880044881.01.T01 MTR_2g082340 92.647 136 10 0 1 136 1 136 4.34e-90 257
MsG0880044881.01.T01 MTR_2g082370 86.301 146 10 1 1 136 1 146 3.95e-84 243
MsG0880044881.01.T01 MTR_2g082400 85.294 136 18 2 1 135 1 135 1.44e-76 223
MsG0880044881.01.T01 MTR_7g013600 83.036 112 19 0 25 136 2 113 1.79e-62 187
MsG0880044881.01.T01 MTR_7g023830 67.164 134 43 1 1 134 1 133 3.94e-56 172
MsG0880044881.01.T01 MTR_1g023630 77.477 111 23 1 1 111 1 109 1.57e-54 167
MsG0880044881.01.T01 MTR_7g023880 60.150 133 39 3 3 134 18 137 2.49e-44 142
MsG0880044881.01.T01 MTR_5g024630 63.793 116 39 2 1 116 1 113 1.60e-42 136
MsG0880044881.01.T01 MTR_5g024530 65.455 110 15 2 19 128 68 154 6.49e-40 131
MsG0880044881.01.T01 MTR_0197s0100 96.970 66 2 0 1 66 1 66 1.91e-39 129
MsG0880044881.01.T01 MTR_8g027840 56.122 98 42 1 37 133 28 125 4.82e-31 108
MsG0880044881.01.T01 MTR_8g105150 59.783 92 3 2 37 127 11 69 1.28e-26 95.1
MsG0880044881.01.T01 MTR_8g092740 97.368 38 1 0 99 136 118 155 5.96e-20 80.5
MsG0880044881.01.T01 MTR_5g089120 68.085 47 15 0 82 128 6 52 1.62e-16 70.1
MsG0880044881.01.T01 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.17e-15 66.6
MsG0880044881.01.T01 MTR_4g128040 72.973 37 10 0 79 115 20 56 6.28e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044881.01.T01 AT5G10980 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40040 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40040 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40040 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40030 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40030 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40030 100.000 136 0 0 1 136 1 136 1.45e-96 274
MsG0880044881.01.T01 AT4G40030 100.000 136 0 0 1 136 29 164 2.12e-96 275
MsG0880044881.01.T01 AT5G65360 97.059 136 4 0 1 136 1 136 4.00e-93 265
MsG0880044881.01.T01 AT5G10390 97.059 136 4 0 1 136 1 136 4.00e-93 265
MsG0880044881.01.T01 AT5G10400 97.059 136 4 0 1 136 1 136 4.00e-93 265
MsG0880044881.01.T01 AT3G27360 97.059 136 4 0 1 136 1 136 4.00e-93 265
MsG0880044881.01.T01 AT1G09200 97.059 136 4 0 1 136 1 136 4.00e-93 265
MsG0880044881.01.T01 AT1G75600 96.324 136 5 0 1 136 1 136 1.06e-92 264
MsG0880044881.01.T01 AT1G13370 94.853 136 7 0 1 136 1 136 2.25e-91 261
MsG0880044881.01.T01 AT5G65350 92.647 136 10 0 1 136 1 136 1.32e-88 254
MsG0880044881.01.T01 AT1G19890 90.511 137 12 1 1 136 1 137 1.97e-85 246
MsG0880044881.01.T01 AT5G12910 71.111 135 34 2 1 135 1 130 1.19e-65 196
MsG0880044881.01.T01 AT1G01370 54.867 113 48 2 24 134 63 174 3.36e-32 112
MsG0880044881.01.T01 AT1G01370 54.867 113 48 2 24 134 63 174 3.36e-32 112
MsG0880044881.01.T01 AT1G01370 54.867 113 48 2 24 134 63 174 3.36e-32 112
MsG0880044881.01.T01 AT1G01370 54.867 113 48 2 24 134 63 174 3.36e-32 112

Find 39 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGCTGTTCTTGCACTTC+AGG 0.281295 8:+50878252 MsG0880044881.01.T01:CDS
TCACGAAGAGCAACGGTTCC+AGG 0.316584 8:-50878121 None:intergenic
AGCAGATTTCCTTGCAGCTT+TGG 0.347069 8:-50878059 None:intergenic
CTTCCTTGGTGCTTTGCCGC+CGG 0.391014 8:-50878026 None:intergenic
GGAAGCGGCTGAGGCTTATC+TGG 0.391484 8:+50878273 MsG0880044881.01.T01:CDS
TATTCGTGGTGAACGTGCTT+AGG 0.412470 8:+50878381 MsG0880044881.01.T01:CDS
TTCTTCACTCCGCCGGTGGT+TGG 0.414328 8:-50878082 None:intergenic
TTATCTGGTGGGATTGTTTG+AGG 0.425602 8:+50878288 MsG0880044881.01.T01:CDS
CAGCTTGCTCGTCGTATTCG+TGG 0.425806 8:+50878367 MsG0880044881.01.T01:CDS
TGCACTTCAGGAAGCGGCTG+AGG 0.430700 8:+50878264 MsG0880044881.01.T01:CDS
TTGGTGGCGAGTTGCTTCCT+TGG 0.434612 8:-50878040 None:intergenic
CCTTGGTGCTTTGCCGCCGG+TGG 0.443251 8:-50878023 None:intergenic
AGATTTCCTTGCAGCTTTGG+TGG 0.476244 8:-50878056 None:intergenic
TCTTGTTCGTGAAATTGCTC+AGG 0.482836 8:+50878201 MsG0880044881.01.T01:CDS
CGGTACTCTTCTGATACTTA+CGG 0.500491 8:-50878147 None:intergenic
AAGCCTCATCGTTATCGTCC+TGG 0.515117 8:+50878103 MsG0880044881.01.T01:CDS
AGGAAATCTGCTCCAACCAC+CGG 0.534350 8:+50878070 MsG0880044881.01.T01:CDS
AATTGCTCAGGATTTCAAAA+CGG 0.537563 8:+50878213 MsG0880044881.01.T01:CDS
AGGCTTCTTCACTCCGCCGG+TGG 0.558370 8:-50878086 None:intergenic
GACGCTGGAATGGAAGCTTA+CGG 0.558442 8:-50878180 None:intergenic
TGTTCTTGCACTTCAGGAAG+CGG 0.559874 8:+50878258 MsG0880044881.01.T01:CDS
CGAGCAAGCTGAATATCCTT+CGG 0.565564 8:-50878355 None:intergenic
TCACGAACAAGACGCTGGAA+TGG 0.567002 8:-50878190 None:intergenic
GCGGCTGAGGCTTATCTGGT+GGG 0.567245 8:+50878277 MsG0880044881.01.T01:CDS
CAAACTGCTCGCAAATCCAC+CGG 0.571388 8:+50878007 MsG0880044881.01.T01:CDS
ATGAGGCTTCTTCACTCCGC+CGG 0.573608 8:-50878089 None:intergenic
AGCGGCTGAGGCTTATCTGG+TGG 0.574844 8:+50878276 MsG0880044881.01.T01:CDS
AGGATTTCAAAACGGATCTG+AGG 0.592218 8:+50878221 MsG0880044881.01.T01:CDS
ACGGATCTCACGAAGAGCAA+CGG 0.595480 8:-50878128 None:intergenic
AACTCGCCACCAAAGCTGCA+AGG 0.602982 8:+50878050 MsG0880044881.01.T01:CDS
CAATTTCACGAACAAGACGC+TGG 0.604340 8:-50878195 None:intergenic
GTGTGCTATTCATGCTAAGA+GGG 0.612146 8:+50878321 MsG0880044881.01.T01:CDS
TGTGTGCTATTCATGCTAAG+AGG 0.615466 8:+50878320 MsG0880044881.01.T01:CDS
GAGGGTGACGATTATGCCGA+AGG 0.625074 8:+50878339 MsG0880044881.01.T01:CDS
CCACCGGCGGCAAAGCACCA+AGG 0.632902 8:+50878023 MsG0880044881.01.T01:CDS
AAGCTTACGGATCAAAAGCT+CGG 0.636318 8:-50878167 None:intergenic
ACTGCTCGCAAATCCACCGG+CGG 0.667900 8:+50878010 MsG0880044881.01.T01:CDS
AAATCTGCTCCAACCACCGG+CGG 0.687441 8:+50878073 MsG0880044881.01.T01:CDS
GTTCCAGGACGATAACGATG+AGG 0.738178 8:-50878106 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATTGCTCAGGATTTCAAAA+CGG + Chr8:50878213-50878232 MsG0880044881.01.T01:CDS 30.0%
AAGCTTACGGATCAAAAGCT+CGG - Chr8:50878170-50878189 None:intergenic 40.0%
AGGATTTCAAAACGGATCTG+AGG + Chr8:50878221-50878240 MsG0880044881.01.T01:CDS 40.0%
CGGTACTCTTCTGATACTTA+CGG - Chr8:50878150-50878169 None:intergenic 40.0%
TCTTGTTCGTGAAATTGCTC+AGG + Chr8:50878201-50878220 MsG0880044881.01.T01:CDS 40.0%
TTATCTGGTGGGATTGTTTG+AGG + Chr8:50878288-50878307 MsG0880044881.01.T01:CDS 40.0%
! GTGTGCTATTCATGCTAAGA+GGG + Chr8:50878321-50878340 MsG0880044881.01.T01:CDS 40.0%
! TGTGTGCTATTCATGCTAAG+AGG + Chr8:50878320-50878339 MsG0880044881.01.T01:CDS 40.0%
AGCAGATTTCCTTGCAGCTT+TGG - Chr8:50878062-50878081 None:intergenic 45.0%
CAATTTCACGAACAAGACGC+TGG - Chr8:50878198-50878217 None:intergenic 45.0%
CGAGCAAGCTGAATATCCTT+CGG - Chr8:50878358-50878377 None:intergenic 45.0%
TCATGCTGTTCTTGCACTTC+AGG + Chr8:50878252-50878271 MsG0880044881.01.T01:CDS 45.0%
TGTTCTTGCACTTCAGGAAG+CGG + Chr8:50878258-50878277 MsG0880044881.01.T01:CDS 45.0%
! AGATTTCCTTGCAGCTTTGG+TGG - Chr8:50878059-50878078 None:intergenic 45.0%
AAGCCTCATCGTTATCGTCC+TGG + Chr8:50878103-50878122 MsG0880044881.01.T01:CDS 50.0%
ACGGATCTCACGAAGAGCAA+CGG - Chr8:50878131-50878150 None:intergenic 50.0%
AGGAAATCTGCTCCAACCAC+CGG + Chr8:50878070-50878089 MsG0880044881.01.T01:CDS 50.0%
CAAACTGCTCGCAAATCCAC+CGG + Chr8:50878007-50878026 MsG0880044881.01.T01:CDS 50.0%
GACGCTGGAATGGAAGCTTA+CGG - Chr8:50878183-50878202 None:intergenic 50.0%
GTTCCAGGACGATAACGATG+AGG - Chr8:50878109-50878128 None:intergenic 50.0%
TCACGAACAAGACGCTGGAA+TGG - Chr8:50878193-50878212 None:intergenic 50.0%
AAATCTGCTCCAACCACCGG+CGG + Chr8:50878073-50878092 MsG0880044881.01.T01:CDS 55.0%
AACTCGCCACCAAAGCTGCA+AGG + Chr8:50878050-50878069 MsG0880044881.01.T01:CDS 55.0%
ATGAGGCTTCTTCACTCCGC+CGG - Chr8:50878092-50878111 None:intergenic 55.0%
GAGGGTGACGATTATGCCGA+AGG + Chr8:50878339-50878358 MsG0880044881.01.T01:CDS 55.0%
TCACGAAGAGCAACGGTTCC+AGG - Chr8:50878124-50878143 None:intergenic 55.0%
! CAGCTTGCTCGTCGTATTCG+TGG + Chr8:50878367-50878386 MsG0880044881.01.T01:CDS 55.0%
!! TTGGTGGCGAGTTGCTTCCT+TGG - Chr8:50878043-50878062 None:intergenic 55.0%
ACTGCTCGCAAATCCACCGG+CGG + Chr8:50878010-50878029 MsG0880044881.01.T01:CDS 60.0%
AGCGGCTGAGGCTTATCTGG+TGG + Chr8:50878276-50878295 MsG0880044881.01.T01:CDS 60.0%
GCGGCTGAGGCTTATCTGGT+GGG + Chr8:50878277-50878296 MsG0880044881.01.T01:CDS 60.0%
GGAAGCGGCTGAGGCTTATC+TGG + Chr8:50878273-50878292 MsG0880044881.01.T01:CDS 60.0%
TGCACTTCAGGAAGCGGCTG+AGG + Chr8:50878264-50878283 MsG0880044881.01.T01:CDS 60.0%
TTCTTCACTCCGCCGGTGGT+TGG - Chr8:50878085-50878104 None:intergenic 60.0%
!! CTTCCTTGGTGCTTTGCCGC+CGG - Chr8:50878029-50878048 None:intergenic 60.0%
AGGCTTCTTCACTCCGCCGG+TGG - Chr8:50878089-50878108 None:intergenic 65.0%
! CCACCGGCGGCAAAGCACCA+AGG + Chr8:50878023-50878042 MsG0880044881.01.T01:CDS 70.0%
!! CCTTGGTGCTTTGCCGCCGG+TGG - Chr8:50878026-50878045 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 50877992 50878402 50877992 ID=MsG0880044881.01;Name=MsG0880044881.01
Chr8 mRNA 50877992 50878402 50877992 ID=MsG0880044881.01.T01;Parent=MsG0880044881.01;Name=MsG0880044881.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|136
Chr8 exon 50877992 50878402 50877992 ID=MsG0880044881.01.T01:exon:21543;Parent=MsG0880044881.01.T01
Chr8 CDS 50877992 50878402 50877992 ID=MsG0880044881.01.T01:cds;Parent=MsG0880044881.01.T01
Gene Sequence

>MsG0880044881.01.T01

ATGGCTCGTACGAAGCAAACTGCTCGCAAATCCACCGGCGGCAAAGCACCAAGGAAGCAACTCGCCACCAAAGCTGCAAGGAAATCTGCTCCAACCACCGGCGGAGTGAAGAAGCCTCATCGTTATCGTCCTGGAACCGTTGCTCTTCGTGAGATCCGTAAGTATCAGAAGAGTACCGAGCTTTTGATCCGTAAGCTTCCATTCCAGCGTCTTGTTCGTGAAATTGCTCAGGATTTCAAAACGGATCTGAGGTTTCAGAGTCATGCTGTTCTTGCACTTCAGGAAGCGGCTGAGGCTTATCTGGTGGGATTGTTTGAGGATACTAATTTGTGTGCTATTCATGCTAAGAGGGTGACGATTATGCCGAAGGATATTCAGCTTGCTCGTCGTATTCGTGGTGAACGTGCTTAG

Protein sequence

>MsG0880044881.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*