AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045535.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045535.01.T01 MTR_8g073345 59.677 124 1 2 1 75 1 124 1.51e-36 120
MsG0880045535.01.T01 MTR_8g073345 59.677 124 1 2 1 75 1 124 2.01e-36 120
MsG0880045535.01.T01 MTR_5g024180 49.367 79 23 2 14 75 10 88 1.84e-16 68.6
MsG0880045535.01.T01 MTR_5g024180 37.600 125 28 3 1 75 1 125 3.08e-15 66.2
MsG0880045535.01.T01 MTR_3g115580 60.784 51 20 0 16 66 58 108 6.15e-15 65.9
MsG0880045535.01.T01 MTR_7g068280 55.556 45 20 0 16 60 64 108 2.22e-11 57.0
MsG0880045535.01.T01 MTR_7g082820 59.574 47 18 1 15 61 25 70 5.35e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045535.01.T01 AT1G20696 50.633 79 23 1 13 75 3 81 5.26e-16 67.4
MsG0880045535.01.T01 AT1G20696 50.633 79 23 1 13 75 3 81 6.14e-16 67.0
MsG0880045535.01.T01 AT1G20696 51.471 68 33 0 16 83 48 115 2.57e-15 66.6
MsG0880045535.01.T01 AT1G20696 51.316 76 21 1 16 75 48 123 4.05e-15 66.2
MsG0880045535.01.T01 AT1G20696 45.714 70 38 0 16 85 48 117 8.49e-15 65.1
MsG0880045535.01.T01 AT1G20693 48.684 76 23 1 16 75 51 126 2.49e-14 64.3
MsG0880045535.01.T01 AT1G20693 48.684 76 23 1 16 75 51 126 2.55e-14 64.3
MsG0880045535.01.T01 AT1G20693 48.684 76 23 1 16 75 51 126 2.90e-14 63.9
MsG0880045535.01.T01 AT2G17560 48.052 77 23 1 16 75 48 124 3.13e-14 63.5
MsG0880045535.01.T01 AT2G17560 48.052 77 23 1 16 75 48 124 3.52e-14 63.5
MsG0880045535.01.T01 AT2G17560 48.052 77 23 1 16 75 48 124 3.52e-14 63.5
MsG0880045535.01.T01 AT3G51880 47.761 67 25 1 16 72 66 132 1.29e-12 60.1
MsG0880045535.01.T01 AT3G51880 47.761 67 25 1 16 72 66 132 1.95e-12 60.1
MsG0880045535.01.T01 AT3G51880 47.761 67 25 1 16 72 66 132 2.35e-12 59.7
MsG0880045535.01.T01 AT3G51880 47.761 67 25 1 16 72 66 132 2.35e-12 59.7
MsG0880045535.01.T01 AT3G51880 47.761 67 25 1 16 72 66 132 2.35e-12 59.7

Find 39 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGGGAGTGAAGGGTTAT+AGG 0.256661 8:+60653158 None:intergenic
GATACTGAACCGTACTTTAG+AGG 0.264950 8:+60651738 None:intergenic
TGTGCGTTATTGTGAGATTT+TGG 0.303526 8:+60653111 None:intergenic
CTTATACTGTTCTCTAAAAT+CGG 0.321982 8:+60652626 None:intergenic
GCGGCAACGGATTTGTTGTT+AGG 0.341320 8:+60652594 None:intergenic
ACCACCACATGATGAAGTTT+AGG 0.342275 8:-60651866 MsG0880045535.01.T01:three_prime_UTR
TTACCGGGTATCAGCGGGTT+TGG 0.344714 8:+60652996 None:intergenic
AGAGGAAAACGCAAAGAAAA+AGG 0.356896 8:+60653181 None:intergenic
TTGGAATCAGCTTTAACTTT+CGG 0.360829 8:+60653015 None:intergenic
CTTCATCCTCATCATTGATT+CGG 0.371386 8:+60652046 None:intergenic
CCACCACATGATGAAGTTTA+GGG 0.386215 8:-60651865 MsG0880045535.01.T01:three_prime_UTR
GAAAGGAGCGGTTGCTGAAT+CGG 0.393709 8:+60653051 None:intergenic
ATAAGAAGGGTAGGGAGTGA+AGG 0.407782 8:+60653150 None:intergenic
TTCAATGCCCTAGCAGCATA+AGG 0.424128 8:+60652258 None:intergenic
CGAACGAGAGAGGGATAAGA+AGG 0.432367 8:+60653136 None:intergenic
GCGATTTACCGGGTATCAGC+GGG 0.444107 8:+60652991 None:intergenic
AAAGGAGCGGTTGCTGAATC+GGG 0.456016 8:+60653052 None:intergenic
GAGAGAGGGATAAGAAGGGT+AGG 0.471864 8:+60653141 None:intergenic
CCCTAAACTTCATCATGTGG+TGG 0.495261 8:+60651865 None:intergenic
GAGATTTCTTACAGCGGCAA+CGG 0.506191 8:+60652581 None:intergenic
CAAGGCTCCTTATGCTGCTA+GGG 0.507356 8:-60652265 MsG0880045535.01.T01:CDS
AGAGAGGGATAAGAAGGGTA+GGG 0.509126 8:+60653142 None:intergenic
ACAAGGCTCCTTATGCTGCT+AGG 0.511412 8:-60652266 MsG0880045535.01.T01:CDS
GAAAAGCTTGCGGTGAAGCA+TGG 0.538505 8:-60652445 MsG0880045535.01.T01:CDS
TAAGAAGGGTAGGGAGTGAA+GGG 0.543568 8:+60653151 None:intergenic
GTGAAGAATCCTCTAAAGTA+CGG 0.552251 8:-60651747 MsG0880045535.01.T01:three_prime_UTR
GGAGTGAAGGGTTATAGGAG+AGG 0.554023 8:+60653163 None:intergenic
AGCGATTTACCGGGTATCAG+CGG 0.554649 8:+60652990 None:intergenic
ATTCCAAACCCGCTGATACC+CGG 0.556619 8:-60652999 MsG0880045535.01.T01:intron
ATCAGCTTTAACTTTCGGCA+TGG 0.563633 8:+60653020 None:intergenic
TCATAACATTGACTTTACAT+AGG 0.564554 8:+60651707 None:intergenic
TAACATTGACTTTACATAGG+AGG 0.564623 8:+60651710 None:intergenic
GAGGGAAAAGACGAGGAAGA+TGG 0.570227 8:-60652086 MsG0880045535.01.T01:CDS
TTTCCCTAAACTTCATCATG+TGG 0.574768 8:+60651862 None:intergenic
CAATGATGAGGATGAAGATG+AGG 0.591186 8:-60652040 MsG0880045535.01.T01:intron
GAACGAGAGAGGGATAAGAA+GGG 0.602505 8:+60653137 None:intergenic
CAAAATCCGAATCAATGATG+AGG 0.606497 8:-60652052 MsG0880045535.01.T01:CDS
TATTAGGTCGGAAAAGCTTG+CGG 0.611496 8:-60652455 MsG0880045535.01.T01:intron
TTATTAGGAGGGAAAAGACG+AGG 0.645171 8:-60652093 MsG0880045535.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTATTTTATTTTT+TGG - Chr8:60652334-60652353 MsG0880045535.01.T01:intron 0.0%
!!! ATTAATTTTTCGAAATTATT+AGG - Chr8:60652401-60652420 MsG0880045535.01.T01:intron 10.0%
!!! GAAAAAAATATGTTTTTTTT+AGG - Chr8:60653041-60653060 MsG0880045535.01.T01:five_prime_UTR 10.0%
!!! TTAATTTTAATTGATGATTT+AGG - Chr8:60652222-60652241 MsG0880045535.01.T01:intron 10.0%
!! ATGATAATTGAATAATTGAA+TGG - Chr8:60652901-60652920 MsG0880045535.01.T01:intron 15.0%
!!! AATATGTTTTTTTTAGGTTA+TGG - Chr8:60653047-60653066 MsG0880045535.01.T01:five_prime_UTR 15.0%
!!! ATAATTTGTGATTTTATTGT+CGG - Chr8:60652360-60652379 MsG0880045535.01.T01:intron 15.0%
!!! TAACTTTTTGTTGATTTATT+AGG - Chr8:60652764-60652783 MsG0880045535.01.T01:intron 15.0%
!!! TTCATTCATATTAGTAATTT+TGG - Chr8:60652691-60652710 MsG0880045535.01.T01:intron 15.0%
!!! TTTTTTTTGTTAACTTTCAT+CGG - Chr8:60652491-60652510 MsG0880045535.01.T01:intron 15.0%
!!! ATTTTTCGAAATTATTAGGT+CGG - Chr8:60652405-60652424 MsG0880045535.01.T01:intron 20.0%
!!! GATTTGTTCGTTTTTTTTAA+CGG - Chr8:60652195-60652214 MsG0880045535.01.T01:intron 20.0%
!!! TTTCAGATTTGAATCTTTTT+TGG - Chr8:60652154-60652173 MsG0880045535.01.T01:intron 20.0%
!!! TTTTTGTTGATTTATTAGGA+GGG - Chr8:60652768-60652787 MsG0880045535.01.T01:intron 20.0%
!!! AGTTTATTTTTAACCCTTGT+AGG - Chr8:60652933-60652952 MsG0880045535.01.T01:intron 25.0%
!!! CTTATACTGTTCTCTAAAAT+CGG + Chr8:60652249-60652268 None:intergenic 25.0%
!!! CTTTTTGTTGATTTATTAGG+AGG - Chr8:60652767-60652786 MsG0880045535.01.T01:intron 25.0%
!!! TCATAACATTGACTTTACAT+AGG + Chr8:60653168-60653187 None:intergenic 25.0%
ATTGGAGAAGAAAAATCGAA+CGG + Chr8:60651913-60651932 None:intergenic 30.0%
GAAGTTTAGGGAAAAATTGT+TGG - Chr8:60653019-60653038 MsG0880045535.01.T01:CDS 30.0%
! TTGGAATCAGCTTTAACTTT+CGG + Chr8:60651860-60651879 None:intergenic 30.0%
!! TAACATTGACTTTACATAGG+AGG + Chr8:60653165-60653184 None:intergenic 30.0%
!!! GTACTGTGATTTTGATTTTC+AGG - Chr8:60652583-60652602 MsG0880045535.01.T01:intron 30.0%
!!! TGATTTTGATTTTCAGGACA+AGG - Chr8:60652589-60652608 MsG0880045535.01.T01:intron 30.0%
AGAGGAAAACGCAAAGAAAA+AGG + Chr8:60651694-60651713 None:intergenic 35.0%
CAAAATCCGAATCAATGATG+AGG - Chr8:60652820-60652839 MsG0880045535.01.T01:intron 35.0%
GTAAACGAGATTTCTTACAG+CGG + Chr8:60652300-60652319 None:intergenic 35.0%
TTTCCCTAAACTTCATCATG+TGG + Chr8:60653013-60653032 None:intergenic 35.0%
! ATTCACCAGTTTCTTGTTGT+AGG + Chr8:60652670-60652689 None:intergenic 35.0%
! CTTCATCCTCATCATTGATT+CGG + Chr8:60652829-60652848 None:intergenic 35.0%
! TGTGCGTTATTGTGAGATTT+TGG + Chr8:60651764-60651783 None:intergenic 35.0%
! TTTTAACCCTTGTAGGAGAT+GGG - Chr8:60652940-60652959 MsG0880045535.01.T01:intron 35.0%
! TTTTTAACCCTTGTAGGAGA+TGG - Chr8:60652939-60652958 MsG0880045535.01.T01:intron 35.0%
!! ATGAAAACGAAGAAAGCACT+TGG + Chr8:60652107-60652126 None:intergenic 35.0%
!! GTGAAGAATCCTCTAAAGTA+CGG - Chr8:60653125-60653144 MsG0880045535.01.T01:five_prime_UTR 35.0%
ACAAGCCTACAACAAGAAAC+TGG - Chr8:60652662-60652681 MsG0880045535.01.T01:intron 40.0%
ACCACCACATGATGAAGTTT+AGG - Chr8:60653006-60653025 MsG0880045535.01.T01:CDS 40.0%
CAATGATGAGGATGAAGATG+AGG - Chr8:60652832-60652851 MsG0880045535.01.T01:intron 40.0%
CCACCACATGATGAAGTTTA+GGG - Chr8:60653007-60653026 MsG0880045535.01.T01:CDS 40.0%
GATACTGAACCGTACTTTAG+AGG + Chr8:60653137-60653156 None:intergenic 40.0%
TATTAGGTCGGAAAAGCTTG+CGG - Chr8:60652417-60652436 MsG0880045535.01.T01:intron 40.0%
TTATTAGGAGGGAAAAGACG+AGG - Chr8:60652779-60652798 MsG0880045535.01.T01:intron 40.0%
! ATCAGCTTTAACTTTCGGCA+TGG + Chr8:60651855-60651874 None:intergenic 40.0%
!! AAAACGAAGAAAGCACTTGG+AGG + Chr8:60652104-60652123 None:intergenic 40.0%
!!! GATTTTAGCTTGAAGCGCAA+AGG - Chr8:60652021-60652040 MsG0880045535.01.T01:intron 40.0%
AACGGTGAGAAGCGATTTAC+CGG + Chr8:60651895-60651914 None:intergenic 45.0%
AGAGAGGGATAAGAAGGGTA+GGG + Chr8:60651733-60651752 None:intergenic 45.0%
ATAAGAAGGGTAGGGAGTGA+AGG + Chr8:60651725-60651744 None:intergenic 45.0%
ATGGCGAAAAACGAAAGGAG+CGG + Chr8:60651836-60651855 None:intergenic 45.0%
CATCATCCCATCTCCTACAA+GGG + Chr8:60652949-60652968 None:intergenic 45.0%
CCCTAAACTTCATCATGTGG+TGG + Chr8:60653010-60653029 None:intergenic 45.0%
GAACGAGAGAGGGATAAGAA+GGG + Chr8:60651738-60651757 None:intergenic 45.0%
GAGATTTCTTACAGCGGCAA+CGG + Chr8:60652294-60652313 None:intergenic 45.0%
TAAGAAGGGTAGGGAGTGAA+GGG + Chr8:60651724-60651743 None:intergenic 45.0%
TCATCATCCCATCTCCTACA+AGG + Chr8:60652950-60652969 None:intergenic 45.0%
TTCAATGCCCTAGCAGCATA+AGG + Chr8:60652617-60652636 None:intergenic 45.0%
! AAAGCACTTGGAGGTCTCTT+TGG + Chr8:60652095-60652114 None:intergenic 45.0%
!!! ATTTTGGAGCGAACGAGAGA+GGG + Chr8:60651748-60651767 None:intergenic 45.0%
!! CTAAAAAATATTAAAAAAAT+TGG + Chr8:60651931-60651950 None:intergenic 5.0%
AAAGGAGCGGTTGCTGAATC+GGG + Chr8:60651823-60651842 None:intergenic 50.0%
ACAAGGCTCCTTATGCTGCT+AGG - Chr8:60652606-60652625 MsG0880045535.01.T01:CDS 50.0%
ACGGTGAGAAGCGATTTACC+GGG + Chr8:60651894-60651913 None:intergenic 50.0%
AGCGATTTACCGGGTATCAG+CGG + Chr8:60651885-60651904 None:intergenic 50.0%
ATTCCAAACCCGCTGATACC+CGG - Chr8:60651873-60651892 MsG0880045535.01.T01:three_prime_UTR 50.0%
CAAGGCTCCTTATGCTGCTA+GGG - Chr8:60652607-60652626 MsG0880045535.01.T01:CDS 50.0%
CGAACGAGAGAGGGATAAGA+AGG + Chr8:60651739-60651758 None:intergenic 50.0%
GAAAAGCTTGCGGTGAAGCA+TGG - Chr8:60652427-60652446 MsG0880045535.01.T01:CDS 50.0%
GAAAGGAGCGGTTGCTGAAT+CGG + Chr8:60651824-60651843 None:intergenic 50.0%
GAGAGAGGGATAAGAAGGGT+AGG + Chr8:60651734-60651753 None:intergenic 50.0%
GAGGGAAAAGACGAGGAAGA+TGG - Chr8:60652786-60652805 MsG0880045535.01.T01:intron 50.0%
GGAGTGAAGGGTTATAGGAG+AGG + Chr8:60651712-60651731 None:intergenic 50.0%
GGTAGGGAGTGAAGGGTTAT+AGG + Chr8:60651717-60651736 None:intergenic 50.0%
TCGGCATGGCGAAAAACGAA+AGG + Chr8:60651841-60651860 None:intergenic 50.0%
!! GCGGCAACGGATTTGTTGTT+AGG + Chr8:60652281-60652300 None:intergenic 50.0%
!! GGAGGTCTCTTTGGCTTGTT+AGG + Chr8:60652086-60652105 None:intergenic 50.0%
!!! GATTTTGGAGCGAACGAGAG+AGG + Chr8:60651749-60651768 None:intergenic 50.0%
GCGATTTACCGGGTATCAGC+GGG + Chr8:60651884-60651903 None:intergenic 55.0%
! AGCTTGAAGCGCAAAGGTGC+TGG - Chr8:60652027-60652046 MsG0880045535.01.T01:intron 55.0%
! TTACCGGGTATCAGCGGGTT+TGG + Chr8:60651879-60651898 None:intergenic 55.0%
!! TGAAGCGCAAAGGTGCTGGC+TGG - Chr8:60652031-60652050 MsG0880045535.01.T01:intron 60.0%
!! GCGCAAAGGTGCTGGCTGGA+AGG - Chr8:60652035-60652054 MsG0880045535.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 60651684 60653210 60651684 ID=MsG0880045535.01;Name=MsG0880045535.01
Chr8 mRNA 60651684 60653210 60651684 ID=MsG0880045535.01.T01;Parent=MsG0880045535.01;Name=MsG0880045535.01.T01;_AED=0.26;_eAED=0.26;_QI=170|0.6|0.83|1|1|1|6|225|92
Chr8 exon 60651684 60651939 60651684 ID=MsG0880045535.01.T01:exon:23259;Parent=MsG0880045535.01.T01
Chr8 exon 60652041 60652108 60652041 ID=MsG0880045535.01.T01:exon:23258;Parent=MsG0880045535.01.T01
Chr8 exon 60652254 60652289 60652254 ID=MsG0880045535.01.T01:exon:23257;Parent=MsG0880045535.01.T01
Chr8 exon 60652427 60652471 60652427 ID=MsG0880045535.01.T01:exon:23256;Parent=MsG0880045535.01.T01
Chr8 exon 60652593 60652650 60652593 ID=MsG0880045535.01.T01:exon:23255;Parent=MsG0880045535.01.T01
Chr8 exon 60653000 60653210 60653000 ID=MsG0880045535.01.T01:exon:23254;Parent=MsG0880045535.01.T01
Chr8 five_prime_UTR 60653041 60653210 60653041 ID=MsG0880045535.01.T01:five_prime_utr;Parent=MsG0880045535.01.T01
Chr8 CDS 60653000 60653040 60653000 ID=MsG0880045535.01.T01:cds;Parent=MsG0880045535.01.T01
Chr8 CDS 60652593 60652650 60652593 ID=MsG0880045535.01.T01:cds;Parent=MsG0880045535.01.T01
Chr8 CDS 60652427 60652471 60652427 ID=MsG0880045535.01.T01:cds;Parent=MsG0880045535.01.T01
Chr8 CDS 60652254 60652289 60652254 ID=MsG0880045535.01.T01:cds;Parent=MsG0880045535.01.T01
Chr8 CDS 60652041 60652108 60652041 ID=MsG0880045535.01.T01:cds;Parent=MsG0880045535.01.T01
Chr8 CDS 60651909 60651939 60651909 ID=MsG0880045535.01.T01:cds;Parent=MsG0880045535.01.T01
Chr8 three_prime_UTR 60651684 60651908 60651684 ID=MsG0880045535.01.T01:three_prime_utr;Parent=MsG0880045535.01.T01
Gene Sequence

>MsG0880045535.01.T01

ATGCCGAAAGTTAAAGCTGATTCCAAACCCGCTGATACCCGGTCCGATTTTAGAGAACAGTATAAGAAAGATCATCCTAACAACAAATCCGTTGCCGCTGTCGGAAAAGCTTGCGGTGAAGCATGGAAATCGATGTCTGAAGAAGACAAGGCTCCTTATGCTGCTAGGGCATTGAAGAAAGAGGGAAAAGACGAGGAAGATGGTTCTGACAAGTCAAAATCCGAATCAATGATGAGGATGAAGATGAGGAGATGGGATGATGATGAAGATGATGACTGA

Protein sequence

>MsG0880045535.01.T01

MPKVKADSKPADTRSDFREQYKKDHPNNKSVAAVGKACGEAWKSMSEEDKAPYAARALKKEGKDEEDGSDKSKSESMMRMKMRRWDDDEDDD*