AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0715150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715150 MtrunA17_Chr5g0402361 94.086 186 11 0 1 186 1 186 9.03e-123 350
Msa0715150 MtrunA17_Chr5g0402361 90.286 175 16 1 15 188 187 361 2.86e-108 314
Msa0715150 MtrunA17_Chr5g0402331 91.005 189 16 1 1 188 1 189 2.41e-121 340
Msa0715150 MtrunA17_Chr4g0044961 44.737 190 95 3 1 181 1 189 3.07e-47 153
Msa0715150 MtrunA17_Chr5g0402351 83.529 85 14 0 32 116 7 91 1.62e-44 143
Msa0715150 MtrunA17_Chr1g0161211 38.983 177 99 5 8 182 12 181 1.14e-31 113
Msa0715150 MtrunA17_Chr3g0111371 38.971 136 80 1 46 181 3 135 5.64e-30 107
Msa0715150 MtrunA17_Chr8g0391691 43.262 141 77 2 48 186 36 175 2.27e-29 107
Msa0715150 MtrunA17_Chr5g0434951 39.259 135 82 0 47 181 3 137 5.02e-28 102
Msa0715150 MtrunA17_Chr8g0392951 35.971 139 89 0 47 185 23 161 2.56e-27 101
Msa0715150 MtrunA17_Chr6g0482731 34.559 136 88 1 47 181 25 160 1.04e-25 97.4
Msa0715150 MtrunA17_Chr1g0169581 34.783 138 87 1 47 181 3 140 7.25e-25 94.4
Msa0715150 MtrunA17_Chr7g0245911 37.430 179 92 6 20 185 23 194 1.57e-24 95.1
Msa0715150 MtrunA17_Chr8g0351681 37.838 148 83 3 48 186 49 196 1.08e-23 92.8
Msa0715150 MtrunA17_Chr4g0033601 37.589 141 85 2 47 184 4 144 9.70e-23 89.0
Msa0715150 MtrunA17_Chr7g0256171 39.583 144 84 2 48 188 5 148 1.12e-21 86.3
Msa0715150 MtrunA17_Chr7g0225921 34.532 139 90 1 45 182 3 141 5.56e-21 84.3
Msa0715150 MtrunA17_Chr1g0169621 31.288 163 84 1 47 181 3 165 6.23e-21 84.7
Msa0715150 MtrunA17_Chr2g0329371 37.762 143 84 2 47 184 63 205 6.66e-21 85.9
Msa0715150 MtrunA17_Chr3g0121191 36.620 142 75 3 48 186 93 222 7.19e-21 86.3
Msa0715150 MtrunA17_Chr1g0188171 34.783 138 83 2 50 183 14 148 2.78e-20 82.8
Msa0715150 MtrunA17_Chr3g0116141 34.091 132 87 0 50 181 7 138 2.83e-20 82.4
Msa0715150 MtrunA17_Chr3g0116151 34.091 132 87 0 50 181 8 139 3.29e-20 82.0
Msa0715150 MtrunA17_Chr6g0459231 32.824 131 88 0 52 182 12 142 6.43e-20 81.6
Msa0715150 MtrunA17_Chr7g0256701 34.286 140 86 3 48 181 73 212 1.62e-19 82.4
Msa0715150 MtrunA17_Chr1g0185001 32.192 146 96 1 40 182 1 146 2.35e-19 80.1
Msa0715150 MtrunA17_Chr3g0093271 30.108 186 117 4 1 182 22 198 7.04e-19 80.1
Msa0715150 MtrunA17_Chr1g0166281 33.813 139 85 2 48 182 12 147 1.38e-18 78.2
Msa0715150 MtrunA17_Chr8g0373691 30.065 153 100 3 36 181 35 187 9.64e-18 77.0
Msa0715150 MtrunA17_Chr4g0057661 32.090 134 91 0 48 181 6 139 1.14e-17 75.5
Msa0715150 MtrunA17_Chr7g0254731 33.094 139 86 2 48 182 12 147 1.17e-17 75.9
Msa0715150 MtrunA17_Chr4g0057641 32.090 134 91 0 48 181 6 139 2.33e-17 74.7
Msa0715150 MtrunA17_Chr5g0440931 33.094 139 86 2 48 182 12 147 4.74e-17 74.3
Msa0715150 MtrunA17_Chr6g0460211 33.094 139 86 2 48 182 12 147 5.38e-17 73.9
Msa0715150 MtrunA17_Chr4g0057651 32.353 136 91 1 48 183 6 140 1.90e-16 72.4
Msa0715150 MtrunA17_Chr4g0064231 31.551 187 105 5 17 184 37 219 2.19e-16 74.3
Msa0715150 MtrunA17_Chr7g0245231 30.968 155 104 3 30 183 13 165 1.92e-15 70.5
Msa0715150 MtrunA17_Chr7g0274101 34.091 132 86 1 53 183 17 148 2.16e-15 69.7
Msa0715150 MtrunA17_Chr6g0450921 30.968 155 104 3 30 183 12 164 2.40e-15 70.1
Msa0715150 MtrunA17_Chr8g0366401 27.536 138 96 1 48 181 17 154 7.00e-15 68.9
Msa0715150 MtrunA17_Chr3g0125311 29.801 151 87 4 48 182 13 160 1.78e-14 67.8
Msa0715150 MtrunA17_Chr4g0062221 27.338 139 96 2 47 181 10 147 3.59e-14 67.0
Msa0715150 MtrunA17_Chr1g0160151 31.293 147 84 4 47 182 21 161 1.01e-13 65.9
Msa0715150 MtrunA17_Chr7g0267751 30.147 136 93 1 49 184 441 574 1.07e-13 68.6
Msa0715150 MtrunA17_Chr1g0176751 30.657 137 91 2 49 184 430 563 1.91e-13 67.8
Msa0715150 MtrunA17_Chr8g0368681 27.517 149 100 4 40 181 1 148 2.11e-13 64.7
Msa0715150 MtrunA17_Chr5g0441691 30.147 136 94 1 49 184 374 508 2.74e-13 67.4
Msa0715150 MtrunA17_Chr1g0170281 30.882 136 92 1 49 184 424 557 2.84e-13 67.4
Msa0715150 MtrunA17_Chr1g0157131 29.710 138 92 2 49 184 380 514 3.58e-13 67.0
Msa0715150 MtrunA17_Chr5g0396261 36.364 88 51 2 97 180 26 112 3.91e-13 63.2
Msa0715150 MtrunA17_Chr5g0448721 30.147 136 93 1 49 184 402 535 7.74e-13 65.9
Msa0715150 MtrunA17_Chr0c02g0489761 30.000 140 97 1 49 188 384 522 1.12e-12 65.5
Msa0715150 MtrunA17_Chr4g0068501 27.815 151 104 3 36 181 4 154 1.14e-12 62.8
Msa0715150 MtrunA17_Chr5g0444171 29.412 136 95 1 49 184 398 532 1.48e-12 65.1
Msa0715150 MtrunA17_Chr1g0173541 33.333 132 88 0 53 184 362 493 2.09e-12 64.7
Msa0715150 MtrunA17_Chr5g0397101 29.710 138 92 2 49 184 372 506 3.19e-12 64.3
Msa0715150 MtrunA17_Chr1g0200601 30.882 136 92 1 49 184 336 469 3.22e-12 64.3
Msa0715150 MtrunA17_Chr4g0074811 28.261 138 94 2 49 184 397 531 5.45e-12 63.5
Msa0715150 MtrunA17_Chr8g0385341 31.618 136 91 1 49 184 336 469 6.16e-12 63.5
Msa0715150 MtrunA17_Chr7g0242561 28.261 138 99 0 47 184 360 497 9.16e-12 62.8
Msa0715150 MtrunA17_Chr5g0405421 31.618 136 80 3 53 181 411 540 1.02e-11 62.8
Msa0715150 MtrunA17_Chr7g0257751 30.303 132 92 0 53 184 368 499 1.37e-11 62.4
Msa0715150 MtrunA17_Chr8g0387671 28.986 138 93 2 49 184 372 506 1.46e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715150 AT2G15680.1 41.489 188 97 3 1 181 1 182 1.54e-43 144
Msa0715150 AT5G37770.1 39.860 143 81 1 47 184 16 158 1.25e-31 112
Msa0715150 AT5G37770.2 39.860 143 81 1 47 184 16 158 1.25e-31 112
Msa0715150 AT1G24620.1 42.553 141 78 2 48 186 37 176 1.40e-31 113
Msa0715150 AT1G18210.2 35.443 158 97 2 24 181 1 153 5.11e-29 106
Msa0715150 AT1G18210.1 35.443 158 97 2 24 181 1 153 5.11e-29 106
Msa0715150 AT1G66400.1 38.298 141 80 2 47 182 14 152 7.26e-29 105
Msa0715150 AT5G17470.1 41.727 139 77 3 52 186 6 144 5.28e-28 102
Msa0715150 AT3G03400.1 43.200 125 66 2 52 172 9 132 2.72e-27 100
Msa0715150 AT2G36180.1 41.549 142 78 3 49 186 1 141 3.24e-26 98.2
Msa0715150 AT5G42380.1 32.044 181 117 2 3 181 8 184 3.45e-26 99.4
Msa0715150 AT1G73630.1 36.301 146 89 2 37 181 8 150 7.55e-26 97.8
Msa0715150 AT2G43290.1 37.931 145 82 2 48 184 65 209 2.87e-23 92.4
Msa0715150 AT3G59440.1 38.028 142 80 3 48 184 51 189 3.00e-23 92.0
Msa0715150 AT3G50770.1 32.759 174 102 3 14 181 39 203 1.54e-22 90.5
Msa0715150 AT1G76650.1 31.609 174 112 2 14 181 4 176 1.63e-22 89.7
Msa0715150 AT1G76650.3 31.609 174 112 2 14 181 4 176 1.63e-22 89.7
Msa0715150 AT4G14640.1 35.000 140 84 2 48 183 13 149 6.23e-21 84.7
Msa0715150 AT1G05990.1 39.007 141 82 2 48 184 5 145 1.15e-20 84.0
Msa0715150 AT3G50360.1 34.783 138 89 1 45 181 24 161 5.49e-20 82.8
Msa0715150 AT1G76640.1 34.641 153 96 2 33 181 6 158 5.72e-20 82.4
Msa0715150 AT3G07490.1 37.143 140 85 2 48 184 5 144 9.49e-20 81.6
Msa0715150 AT4G03290.1 36.111 144 86 2 48 185 5 148 8.14e-19 79.3
Msa0715150 AT3G22930.1 34.532 139 89 2 48 184 35 173 8.67e-19 79.7
Msa0715150 AT4G14640.2 34.127 126 76 2 62 183 9 131 1.01e-18 78.6
Msa0715150 AT2G41410.1 30.573 157 98 4 36 182 53 208 1.78e-17 77.4
Msa0715150 AT1G76650.2 30.286 175 103 4 14 181 4 166 2.07e-17 75.9
Msa0715150 AT2G27030.3 32.867 143 89 2 48 186 12 151 3.13e-17 75.9
Msa0715150 AT5G21274.1 33.813 139 85 2 48 182 12 147 3.19e-17 75.1
Msa0715150 AT1G66410.1 33.094 139 86 2 48 182 12 147 3.70e-17 74.7
Msa0715150 AT5G37780.1 33.094 139 86 2 48 182 12 147 3.70e-17 74.7
Msa0715150 AT3G43810.2 32.653 147 90 3 48 188 12 155 4.11e-17 76.3
Msa0715150 AT4G12860.1 36.620 142 87 2 46 184 3 144 4.13e-17 74.7
Msa0715150 AT3G56800.1 33.813 139 85 2 48 182 12 147 4.38e-17 74.7
Msa0715150 AT2G41110.1 33.813 139 85 2 48 182 12 147 4.38e-17 74.7
Msa0715150 AT2G27030.1 33.813 139 85 2 48 182 12 147 4.38e-17 74.7
Msa0715150 AT3G43810.1 33.094 139 86 2 48 182 12 147 7.43e-17 73.9
Msa0715150 AT3G03410.1 34.586 133 79 1 50 182 4 128 2.79e-16 72.0
Msa0715150 AT1G32250.1 29.452 146 88 3 47 181 15 156 3.63e-16 72.8
Msa0715150 AT3G10190.1 30.882 136 92 2 48 181 70 205 6.50e-16 72.8
Msa0715150 AT5G44460.1 36.527 167 86 9 32 181 11 174 2.27e-15 70.9
Msa0715150 AT5G04870.1 32.117 137 89 2 49 184 456 589 7.34e-15 72.4
Msa0715150 AT4G04695.1 33.088 136 90 1 49 184 337 471 1.06e-14 71.6
Msa0715150 AT2G17290.1 33.088 136 89 1 49 184 391 524 1.67e-14 71.2
Msa0715150 AT2G17290.2 33.088 136 89 1 49 184 391 524 1.67e-14 71.2
Msa0715150 AT5G23580.1 32.331 133 88 1 49 181 328 458 1.91e-14 70.9
Msa0715150 AT4G37010.1 28.261 138 98 1 45 181 24 161 3.83e-14 67.4
Msa0715150 AT4G37010.2 28.261 138 98 1 45 181 28 165 4.05e-14 67.4
Msa0715150 AT2G41110.2 31.126 151 85 3 48 182 12 159 4.05e-14 67.0
Msa0715150 AT1G18530.1 31.159 138 90 3 48 181 7 143 4.96e-14 67.0
Msa0715150 AT5G19360.1 31.618 136 92 1 49 184 374 508 5.04e-14 69.7
Msa0715150 AT5G37780.4 32.000 125 78 2 62 182 13 134 5.28e-14 66.2
Msa0715150 AT5G12180.1 31.618 136 92 1 49 184 379 513 5.40e-14 69.7
Msa0715150 AT1G66410.2 32.000 125 78 2 62 182 36 157 5.79e-14 66.6
Msa0715150 AT5G37780.3 32.000 125 78 2 62 182 52 173 7.05e-14 67.0
Msa0715150 AT3G03000.1 30.714 140 93 3 48 184 20 158 8.29e-14 66.2
Msa0715150 AT5G37780.2 32.000 125 78 2 62 182 41 162 9.91e-14 66.2
Msa0715150 AT3G10660.2 30.882 136 92 1 49 184 492 625 1.11e-13 68.9
Msa0715150 AT3G10660.1 30.882 136 92 1 49 184 492 625 1.11e-13 68.9
Msa0715150 AT2G41100.7 30.921 152 87 3 48 181 66 217 1.12e-13 68.2
Msa0715150 AT2G41100.6 30.921 152 87 3 48 181 66 217 1.12e-13 68.2
Msa0715150 AT2G41100.3 30.921 152 87 3 48 181 66 217 1.12e-13 68.2
Msa0715150 AT1G21550.1 31.293 147 90 4 47 183 9 154 1.17e-13 65.9
Msa0715150 AT1G66410.4 38.710 93 50 2 94 182 22 111 1.28e-13 64.7
Msa0715150 AT1G66410.3 38.710 93 50 2 94 182 22 111 1.28e-13 64.7
Msa0715150 AT5G12480.2 33.577 137 90 1 49 184 271 407 1.30e-13 68.6
Msa0715150 AT2G41100.4 31.410 156 88 4 48 185 101 255 1.38e-13 68.2
Msa0715150 AT2G41100.4 29.801 151 89 3 48 181 12 162 3.05e-11 61.2
Msa0715150 AT2G41100.1 31.410 156 88 4 48 185 101 255 1.38e-13 68.2
Msa0715150 AT2G41100.1 29.801 151 89 3 48 181 12 162 3.05e-11 61.2
Msa0715150 AT4G04720.2 31.618 136 92 1 49 184 385 519 1.64e-13 68.6
Msa0715150 AT4G04720.1 31.618 136 92 1 49 184 385 519 1.64e-13 68.6
Msa0715150 AT4G38230.1 33.088 136 89 1 49 184 186 319 1.71e-13 67.8
Msa0715150 AT5G12480.1 33.577 137 90 1 49 184 365 501 1.95e-13 68.2
Msa0715150 AT3G43810.4 38.710 93 50 2 94 182 22 111 2.39e-13 63.9
Msa0715150 AT3G43810.3 38.710 93 50 2 94 182 22 111 2.39e-13 63.9
Msa0715150 AT4G38230.4 33.088 136 89 1 49 184 330 463 2.59e-13 67.8
Msa0715150 AT4G38230.3 33.088 136 89 1 49 184 330 463 2.59e-13 67.8
Msa0715150 AT4G38230.2 33.088 136 89 1 49 184 360 493 2.64e-13 67.8
Msa0715150 AT4G09570.1 33.088 136 89 2 49 184 331 464 2.74e-13 67.8
Msa0715150 AT2G17890.1 34.559 136 76 3 53 181 420 549 5.30e-13 67.0
Msa0715150 AT1G35670.1 32.353 136 90 2 49 184 332 465 6.39e-13 66.6
Msa0715150 AT4G36070.1 33.099 142 82 4 47 181 377 512 7.70e-13 66.2
Msa0715150 AT4G04700.1 32.331 133 89 1 49 181 337 468 7.83e-13 66.2
Msa0715150 AT4G36070.2 33.099 142 82 4 47 181 377 512 9.18e-13 66.2
Msa0715150 AT2G38910.1 31.111 135 91 1 49 183 440 572 1.13e-12 65.9
Msa0715150 AT3G51850.1 28.986 138 98 0 47 184 358 495 1.13e-12 65.9
Msa0715150 AT4G20780.1 30.508 177 95 5 26 181 14 183 1.35e-12 63.5
Msa0715150 AT4G23650.1 31.159 138 90 2 49 184 384 518 1.58e-12 65.5
Msa0715150 AT4G04710.2 31.618 136 92 1 49 184 338 472 1.62e-12 65.5
Msa0715150 AT3G25600.1 28.986 138 93 3 48 181 12 148 1.70e-12 62.8
Msa0715150 AT4G35310.2 30.882 136 92 1 49 184 403 536 1.77e-12 65.5
Msa0715150 AT4G35310.1 30.882 136 92 1 49 184 403 536 1.77e-12 65.5
Msa0715150 AT4G04710.3 31.618 136 92 1 49 184 324 458 1.97e-12 65.1
Msa0715150 AT4G04710.1 31.618 136 92 1 49 184 351 485 2.23e-12 65.1
Msa0715150 AT2G41090.1 29.630 135 93 1 48 182 12 144 2.50e-12 63.2
Msa0715150 AT4G04710.4 31.884 138 91 2 49 184 351 487 4.12e-12 64.3
Msa0715150 AT4G04740.4 28.986 138 93 2 49 184 374 508 4.90e-12 63.9
Msa0715150 AT4G04740.6 28.986 138 93 2 49 184 374 508 4.90e-12 63.9
Msa0715150 AT4G04740.5 28.986 138 93 2 49 184 374 508 4.90e-12 63.9
Msa0715150 AT4G04740.3 28.986 138 93 2 49 184 374 508 4.90e-12 63.9
Msa0715150 AT4G04740.1 28.986 138 93 2 49 184 374 508 4.90e-12 63.9
Msa0715150 AT4G04740.7 28.986 138 93 2 49 184 374 508 4.90e-12 63.9
Msa0715150 AT3G57530.1 33.083 133 86 2 53 184 373 503 5.14e-12 63.9
Msa0715150 AT1G50700.1 30.147 136 94 1 49 184 379 513 1.20e-11 62.8
Msa0715150 AT5G66210.1 31.655 139 76 4 53 181 374 503 1.35e-11 62.8
Msa0715150 AT5G66210.2 31.655 139 76 4 53 181 374 503 1.35e-11 62.8
Msa0715150 AT1G18890.1 30.539 167 109 6 22 184 341 504 1.76e-11 62.4
Msa0715150 AT4G21940.2 30.435 138 91 2 49 184 407 541 3.11e-11 61.6
Msa0715150 AT4G21940.1 30.882 136 93 1 49 184 407 541 3.19e-11 61.6

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCATTTCATCTTTCTT+TGG 0.177414 5_1:-8058495 None:intergenic
TTTCAAGTATTTGATATAAA+TGG 0.216373 5_1:+8058738 Msa0715150:CDS
CGTAGACTTTAAAGAGTTCA+TGG 0.279918 5_1:+8058662 Msa0715150:CDS
TTGAGTTTCCTTAAGTTTCA+TGG 0.307474 5_1:+8058375 Msa0715150:CDS
CCTTAGTGCTTGTAAGAAAA+TGG 0.341545 5_1:+8058824 Msa0715150:CDS
CAAAGAAAGATGAAATGAAA+TGG 0.359646 5_1:+8058496 Msa0715150:CDS
CTTTGGTTGGAAGCTCATTT+CGG 0.372802 5_1:-8058478 None:intergenic
GCTGCAAAAGCTTTGGATAA+GGG 0.402200 5_1:+8058579 Msa0715150:CDS
TAAAGCAGCTGCAAAAGCTT+TGG 0.411228 5_1:+8058572 Msa0715150:CDS
TCATTTCATCTTTCTTTGGT+TGG 0.420505 5_1:-8058491 None:intergenic
AAGCTTTGGATAAGGGGATT+GGG 0.425029 5_1:+8058586 Msa0715150:CDS
AAAAGCTACCATGAAACTTA+AGG 0.427001 5_1:-8058383 None:intergenic
AACTGTCTCAAATATTTAAG+AGG 0.427192 5_1:+8058784 Msa0715150:CDS
AGCTGCAAAAGCTTTGGATA+AGG 0.434736 5_1:+8058578 Msa0715150:CDS
CAAAGATGGCAAGATTAGTC+TGG 0.440035 5_1:+8058542 Msa0715150:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.448139 5_1:+8058789 Msa0715150:CDS
AAAGCTTTGGATAAGGGGAT+TGG 0.463563 5_1:+8058585 Msa0715150:CDS
GTGAAAGGGGTTGACGGTGA+TGG 0.473054 5_1:+8058846 Msa0715150:CDS
AAAGAAAGATGAAATGAAAT+GGG 0.477872 5_1:+8058497 Msa0715150:CDS
CTTGTAAGAAAATGGTGAAA+GGG 0.492061 5_1:+8058832 Msa0715150:CDS
CTGCAAAAGCTTTGGATAAG+GGG 0.499395 5_1:+8058580 Msa0715150:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_1:+8058880 Msa0715150:CDS
AAAATGGTGAAAGGGGTTGA+CGG 0.504340 5_1:+8058840 Msa0715150:CDS
GCCATCTTTGTCAGAGCCCA+TGG 0.521936 5_1:-8058637 None:intergenic
AGCTTTGGATAAGGGGATTG+GGG 0.547088 5_1:+8058587 Msa0715150:CDS
GTATTTGATATAAATGGTGA+TGG 0.547891 5_1:+8058744 Msa0715150:CDS
GAGTTCATGGAAATGTTCAA+TGG 0.548455 5_1:+8058675 Msa0715150:CDS
AATGGCAAGAAATGTGCCTA+AGG 0.556472 5_1:+8058912 Msa0715150:CDS
GGGTAGTAAGATAAAAGAAG+AGG 0.559838 5_1:+8058701 Msa0715150:CDS
AGTGAAAGCATTTAAAGCCA+TGG 0.561156 5_1:+8058620 Msa0715150:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.569070 5_1:+8058831 Msa0715150:CDS
GTGAAAGCATTTAAAGCCAT+GGG 0.572114 5_1:+8058621 Msa0715150:CDS
GGGGTTGACGGTGATGGAGA+TGG 0.573082 5_1:+8058852 Msa0715150:CDS
AAATTTGACACAAACAAAGA+TGG 0.579839 5_1:+8058528 Msa0715150:CDS
ATGGAAATGTTCAATGGAGA+GGG 0.592453 5_1:+8058681 Msa0715150:CDS
CATGGAAATGTTCAATGGAG+AGG 0.594726 5_1:+8058680 Msa0715150:CDS
TTTACAAGGATGATGATGAA+TGG 0.601621 5_1:+8058894 Msa0715150:CDS
TATTTGATATAAATGGTGAT+GGG 0.605312 5_1:+8058745 Msa0715150:CDS
GCCATGGGCTCTGACAAAGA+TGG 0.632822 5_1:+8058636 Msa0715150:CDS
TTGTAAGAAAATGGTGAAAG+GGG 0.653751 5_1:+8058833 Msa0715150:CDS
TGATGGGAAAATCAATGCTG+AGG 0.707496 5_1:+8058761 Msa0715150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAGTATTTGATATAAA+TGG + chr5_1:8058738-8058757 Msa0715150:CDS 15.0%
!! AAAGAAAGATGAAATGAAAT+GGG + chr5_1:8058497-8058516 Msa0715150:CDS 20.0%
!! TATTTGATATAAATGGTGAT+GGG + chr5_1:8058745-8058764 Msa0715150:CDS 20.0%
!!! TTTTGAGCTATATTTTTTCG+TGG - chr5_1:8058426-8058445 None:intergenic 25.0%
! CATTTCATTTCATCTTTCTT+TGG - chr5_1:8058498-8058517 None:intergenic 25.0%
! CAAAGAAAGATGAAATGAAA+TGG + chr5_1:8058496-8058515 Msa0715150:CDS 25.0%
! AAATTTGACACAAACAAAGA+TGG + chr5_1:8058528-8058547 Msa0715150:CDS 25.0%
! GTATTTGATATAAATGGTGA+TGG + chr5_1:8058744-8058763 Msa0715150:CDS 25.0%
! AACTGTCTCAAATATTTAAG+AGG + chr5_1:8058784-8058803 Msa0715150:CDS 25.0%
! TCTCAAATATTTAAGAGGTT+AGG + chr5_1:8058789-8058808 Msa0715150:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG + chr5_1:8058880-8058899 Msa0715150:CDS 25.0%
TTGAGTTTCCTTAAGTTTCA+TGG + chr5_1:8058375-8058394 Msa0715150:CDS 30.0%
AAAAGCTACCATGAAACTTA+AGG - chr5_1:8058386-8058405 None:intergenic 30.0%
!!! ATATTTTTTCGTGGAGATGT+TGG - chr5_1:8058417-8058436 None:intergenic 30.0%
TCATTTCATCTTTCTTTGGT+TGG - chr5_1:8058494-8058513 None:intergenic 30.0%
CTTGTAAGAAAATGGTGAAA+GGG + chr5_1:8058832-8058851 Msa0715150:CDS 30.0%
TTGTAAGAAAATGGTGAAAG+GGG + chr5_1:8058833-8058852 Msa0715150:CDS 30.0%
! TTTACAAGGATGATGATGAA+TGG + chr5_1:8058894-8058913 Msa0715150:CDS 30.0%
!! AGTGAAAGCATTTAAAGCCA+TGG + chr5_1:8058620-8058639 Msa0715150:CDS 35.0%
!! GTGAAAGCATTTAAAGCCAT+GGG + chr5_1:8058621-8058640 Msa0715150:CDS 35.0%
! CGTAGACTTTAAAGAGTTCA+TGG + chr5_1:8058662-8058681 Msa0715150:CDS 35.0%
GAGTTCATGGAAATGTTCAA+TGG + chr5_1:8058675-8058694 Msa0715150:CDS 35.0%
ATGGAAATGTTCAATGGAGA+GGG + chr5_1:8058681-8058700 Msa0715150:CDS 35.0%
GGGTAGTAAGATAAAAGAAG+AGG + chr5_1:8058701-8058720 Msa0715150:CDS 35.0%
! CCATTTTCTTACAAGCACTA+AGG - chr5_1:8058827-8058846 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG + chr5_1:8058824-8058843 Msa0715150:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG + chr5_1:8058831-8058850 Msa0715150:CDS 35.0%
CTTTGGTTGGAAGCTCATTT+CGG - chr5_1:8058481-8058500 None:intergenic 40.0%
! CAAAGATGGCAAGATTAGTC+TGG + chr5_1:8058542-8058561 Msa0715150:CDS 40.0%
TAAAGCAGCTGCAAAAGCTT+TGG + chr5_1:8058572-8058591 Msa0715150:CDS 40.0%
!! AGCTGCAAAAGCTTTGGATA+AGG + chr5_1:8058578-8058597 Msa0715150:CDS 40.0%
!! GCTGCAAAAGCTTTGGATAA+GGG + chr5_1:8058579-8058598 Msa0715150:CDS 40.0%
!! CTGCAAAAGCTTTGGATAAG+GGG + chr5_1:8058580-8058599 Msa0715150:CDS 40.0%
!! AAAGCTTTGGATAAGGGGAT+TGG + chr5_1:8058585-8058604 Msa0715150:CDS 40.0%
!! AAGCTTTGGATAAGGGGATT+GGG + chr5_1:8058586-8058605 Msa0715150:CDS 40.0%
CATGGAAATGTTCAATGGAG+AGG + chr5_1:8058680-8058699 Msa0715150:CDS 40.0%
TGATGGGAAAATCAATGCTG+AGG + chr5_1:8058761-8058780 Msa0715150:CDS 40.0%
AAAATGGTGAAAGGGGTTGA+CGG + chr5_1:8058840-8058859 Msa0715150:CDS 40.0%
!! TTTTCGTGGAGATGTTGGCT+TGG - chr5_1:8058412-8058431 None:intergenic 45.0%
!! AGCTTTGGATAAGGGGATTG+GGG + chr5_1:8058587-8058606 Msa0715150:CDS 45.0%
! GCCATGGGCTCTGACAAAGA+TGG + chr5_1:8058636-8058655 Msa0715150:CDS 55.0%
GCCATCTTTGTCAGAGCCCA+TGG - chr5_1:8058640-8058659 None:intergenic 55.0%
GTGAAAGGGGTTGACGGTGA+TGG + chr5_1:8058846-8058865 Msa0715150:CDS 55.0%
!! GGGGTTGACGGTGATGGAGA+TGG + chr5_1:8058852-8058871 Msa0715150:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 8058366 8058932 8058366 ID=Msa0715150;Name=Msa0715150
chr5_1 mRNA 8058366 8058932 8058366 ID=Msa0715150-mRNA-1;Parent=Msa0715150;Name=Msa0715150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|188
chr5_1 exon 8058366 8058932 8058366 ID=Msa0715150-mRNA-1:exon:3317;Parent=Msa0715150-mRNA-1
chr5_1 CDS 8058366 8058932 8058366 ID=Msa0715150-mRNA-1:cds;Parent=Msa0715150-mRNA-1
Gene Sequence

>Msa0715150

ATGTCAAAATTGAGTTTCCTTAAGTTTCATGGTAGCTTTTCAGCCAAGCCAACATCTCCACGAAAAAATATAGCTCAAAAGAACAGCTTCAAGTCGAGACAAACATCGAGTACCGAAATGAGCTTCCAACCAAAGAAAGATGAAATGAAATGGGTGTTCGAGAAATTTGACACAAACAAAGATGGCAAGATTAGTCTGGAAGAGTATAAAGCAGCTGCAAAAGCTTTGGATAAGGGGATTGGGGATACTGATGCAGTGAAAGCATTTAAAGCCATGGGCTCTGACAAAGATGGCTTCGTAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAGGGTAGTAAGATAAAAGAAGAGGAGATTAAGAGTGCTTTTCAAGTATTTGATATAAATGGTGATGGGAAAATCAATGCTGAGGAACTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGGGTTGACGGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA
Protein sequence

>Msa0715150

MSKLSFLKFHGSFSAKPTSPRKNIAQKNSFKSRQTSSTEMSFQPKKDEMKWVFEKFDTNKDGKISLEEYKAAAKALDKGIGDTDAVKAFKAMGSDKDGFVDFKEFMEMFNGEGSKIKEEEIKSAFQVFDINGDGKINAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRMMMNGKKCA*