AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0163010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163010 MtrunA17_Chr1g0177591 94.433 503 28 0 1 503 72 574 0.0 979
Msa0163010 MtrunA17_Chr1g0177611 94.382 445 25 0 59 503 1 445 0.0 870
Msa0163010 MtrunA17_Chr1g0177631 93.483 445 29 0 59 503 1 445 0.0 832
Msa0163010 MtrunA17_Chr2g0331511 73.512 521 119 4 1 502 51 571 0.0 784
Msa0163010 MtrunA17_Chr2g0331471 70.385 520 135 4 1 501 118 637 0.0 746
Msa0163010 MtrunA17_Chr2g0331481 70.192 520 136 4 1 501 55 574 0.0 742
Msa0163010 MtrunA17_Chr4g0003761 68.015 519 149 4 1 502 60 578 0.0 703
Msa0163010 MtrunA17_Chr2g0331461 67.179 521 152 3 1 502 55 575 0.0 697
Msa0163010 MtrunA17_Chr2g0331521 57.280 522 204 5 1 503 52 573 0.0 593
Msa0163010 MtrunA17_Chr8g0390331 46.680 512 253 8 1 494 45 554 1.31e-158 462
Msa0163010 MtrunA17_Chr1g0157601 44.660 515 263 6 1 496 45 556 3.35e-157 459
Msa0163010 MtrunA17_Chr1g0147631 48.276 522 249 7 1 503 55 574 3.10e-153 449
Msa0163010 MtrunA17_Chr1g0157611 46.139 518 257 7 1 499 46 560 9.70e-151 442
Msa0163010 MtrunA17_Chr1g0157621 44.788 518 263 7 1 499 10 523 9.62e-147 432
Msa0163010 MtrunA17_Chr4g0059971 37.044 521 297 11 1 493 64 581 1.52e-107 332
Msa0163010 MtrunA17_Chr5g0415791 35.135 518 307 9 2 493 60 574 1.28e-105 328
Msa0163010 MtrunA17_Chr4g0059991 34.791 526 303 11 1 493 52 570 5.18e-105 326
Msa0163010 MtrunA17_Chr4g0059981 36.154 520 303 10 1 493 61 578 4.80e-104 323
Msa0163010 MtrunA17_Chr5g0422881 34.556 518 308 10 1 493 50 561 1.56e-99 311
Msa0163010 MtrunA17_Chr3g0104011 36.381 514 301 10 1 493 52 560 3.56e-97 305
Msa0163010 MtrunA17_Chr8g0375311 36.024 508 296 12 10 493 77 579 1.99e-96 303
Msa0163010 MtrunA17_Chr7g0255601 32.115 520 319 11 1 493 57 569 2.88e-95 301
Msa0163010 MtrunA17_Chr7g0255621 31.767 532 326 14 1 503 50 573 2.85e-92 293
Msa0163010 MtrunA17_Chr4g0005901 35.673 513 304 11 1 493 62 568 8.27e-92 291
Msa0163010 MtrunA17_Chr8g0375351 34.834 511 298 11 10 493 77 579 1.98e-91 290
Msa0163010 MtrunA17_Chr8g0362801 36.066 427 256 7 81 493 35 458 2.41e-91 286
Msa0163010 MtrunA17_Chr8g0375341 35.167 509 299 11 10 493 77 579 4.46e-91 289
Msa0163010 MtrunA17_Chr8g0375321 35.352 512 298 12 10 495 74 578 7.41e-91 288
Msa0163010 MtrunA17_Chr8g0375331 35.861 488 284 10 31 495 84 565 1.79e-90 287
Msa0163010 MtrunA17_Chr8g0362781 33.077 520 318 11 2 493 61 578 4.42e-90 286
Msa0163010 MtrunA17_Chr8g0375291 35.317 504 295 12 10 488 77 574 5.78e-90 286
Msa0163010 MtrunA17_Chr7g0241741 36.495 485 280 11 24 487 75 552 8.51e-90 285
Msa0163010 MtrunA17_Chr7g0255611 30.192 520 329 11 1 493 59 571 5.38e-85 274
Msa0163010 MtrunA17_Chr8g0375361 34.979 486 287 11 31 493 99 578 8.13e-85 273
Msa0163010 MtrunA17_Chr4g0060001 32.072 502 313 12 24 502 82 578 6.35e-81 263
Msa0163010 MtrunA17_Chr3g0142101 32.158 482 301 9 25 490 89 560 2.47e-80 261
Msa0163010 MtrunA17_Chr4g0008651 32.621 515 313 13 1 493 64 566 2.56e-80 261
Msa0163010 MtrunA17_Chr5g0415841 30.607 428 282 6 78 493 32 456 2.72e-78 252
Msa0163010 MtrunA17_Chr3g0077151 31.028 506 313 10 1 493 58 540 2.77e-78 254
Msa0163010 MtrunA17_Chr4g0014871 30.392 510 319 8 1 493 60 550 1.47e-77 253
Msa0163010 MtrunA17_Chr4g0014781 32.024 509 313 13 1 493 59 550 1.92e-77 253
Msa0163010 MtrunA17_Chr5g0399061 31.698 530 308 15 1 493 57 569 1.97e-77 253
Msa0163010 MtrunA17_Chr7g0240381 31.041 509 323 11 1 493 59 555 4.39e-77 253
Msa0163010 MtrunA17_Chr4g0014791 31.496 508 315 9 1 493 59 548 4.40e-77 252
Msa0163010 MtrunA17_Chr3g0112671 31.393 481 310 7 24 493 86 557 9.04e-77 251
Msa0163010 MtrunA17_Chr4g0014801 31.299 508 316 12 1 493 59 548 1.29e-74 245
Msa0163010 MtrunA17_Chr4g0014841 31.028 506 318 9 1 493 59 546 2.33e-74 244
Msa0163010 MtrunA17_Chr3g0142091 32.136 501 305 14 24 503 79 565 6.71e-74 244
Msa0163010 MtrunA17_Chr3g0112611 32.454 493 297 11 25 493 86 566 3.67e-73 243
Msa0163010 MtrunA17_Chr2g0325951 32.685 514 307 13 1 493 49 544 4.42e-73 241
Msa0163010 MtrunA17_Chr7g0240281 30.469 512 329 11 1 487 54 563 4.92e-73 242
Msa0163010 MtrunA17_Chr7g0214731 30.930 527 318 12 1 493 95 609 2.24e-72 241
Msa0163010 MtrunA17_Chr2g0284701 29.720 535 339 12 4 503 65 597 3.77e-72 240
Msa0163010 MtrunA17_Chr7g0240271 29.608 510 334 11 1 487 54 561 8.04e-72 239
Msa0163010 MtrunA17_Chr5g0400371 30.041 486 313 11 32 493 173 655 4.45e-70 236
Msa0163010 MtrunA17_Chr2g0325961 33.204 515 304 13 1 493 49 545 3.33e-69 231
Msa0163010 MtrunA17_Chr8g0353031 32.716 486 288 10 25 487 72 541 8.36e-69 230
Msa0163010 MtrunA17_Chr7g0261811 30.280 535 326 14 1 503 57 576 9.91e-69 231
Msa0163010 MtrunA17_Chr4g0063591 31.703 511 329 9 1 495 52 558 1.08e-68 230
Msa0163010 MtrunA17_Chr7g0214461 30.350 514 327 12 1 493 108 611 2.02e-68 231
Msa0163010 MtrunA17_Chr1g0213711 30.977 481 305 10 32 493 82 554 2.31e-68 229
Msa0163010 MtrunA17_Chr3g0114501 30.801 487 303 13 31 493 82 558 4.15e-68 229
Msa0163010 MtrunA17_Chr5g0439381 29.961 514 333 10 1 499 58 559 4.19e-68 229
Msa0163010 MtrunA17_Chr7g0261881 30.801 487 296 12 30 488 86 559 9.23e-68 228
Msa0163010 MtrunA17_Chr2g0326211 32.685 514 307 13 1 493 49 544 1.25e-67 227
Msa0163010 MtrunA17_Chr1g0193391 29.630 513 337 12 1 499 47 549 2.00e-67 228
Msa0163010 MtrunA17_Chr4g0014861 30.930 430 272 7 76 493 9 425 3.31e-67 223
Msa0163010 MtrunA17_Chr7g0271221 30.409 513 333 12 1 499 47 549 3.16e-66 224
Msa0163010 MtrunA17_Chr1g0187241 30.550 491 300 11 31 493 86 563 6.06e-66 223
Msa0163010 MtrunA17_Chr4g0056431 30.019 523 323 14 1 490 55 567 9.00e-66 223
Msa0163010 MtrunA17_Chr7g0240211 31.106 434 281 9 70 487 19 450 3.96e-65 218
Msa0163010 MtrunA17_Chr7g0240181 31.106 434 281 9 70 487 19 450 4.18e-65 218
Msa0163010 MtrunA17_Chr7g0240131 31.106 434 281 9 70 487 19 450 5.91e-65 218
Msa0163010 MtrunA17_Chr5g0399071 30.189 530 316 17 1 493 55 567 6.83e-65 220
Msa0163010 MtrunA17_Chr7g0235521 31.106 434 281 9 70 487 19 450 8.53e-65 218
Msa0163010 MtrunA17_Chr4g0054891 30.083 482 307 13 32 490 171 645 1.68e-64 221
Msa0163010 MtrunA17_Chr4g0011471 30.000 490 319 12 25 493 70 556 3.54e-64 218
Msa0163010 MtrunA17_Chr1g0147591 51.010 198 92 4 87 281 5 200 4.21e-64 207
Msa0163010 MtrunA17_Chr7g0261771 29.344 518 325 12 1 490 61 565 6.04e-64 218
Msa0163010 MtrunA17_Chr3g0133341 30.682 528 320 13 1 499 44 554 1.20e-63 216
Msa0163010 MtrunA17_Chr1g0166741 30.901 466 290 12 31 476 87 540 1.77e-63 216
Msa0163010 MtrunA17_Chr8g0379991 31.673 562 314 19 4 498 13 571 2.46e-63 216
Msa0163010 MtrunA17_Chr1g0166791 30.408 490 302 12 31 493 84 561 3.97e-63 216
Msa0163010 MtrunA17_Chr1g0166771 29.399 466 297 11 31 476 51 504 6.41e-63 214
Msa0163010 MtrunA17_Chr3g0082631 32.184 435 251 12 86 490 33 453 6.90e-63 212
Msa0163010 MtrunA17_Chr2g0276751 30.443 519 331 13 1 503 68 572 9.19e-63 215
Msa0163010 MtrunA17_Chr7g0218851 29.207 517 331 13 1 493 76 581 9.56e-63 215
Msa0163010 MtrunA17_Chr8g0379971 30.556 540 321 15 4 493 53 588 5.91e-62 213
Msa0163010 MtrunA17_Chr7g0261791 30.675 489 299 10 30 490 88 564 6.74e-61 210
Msa0163010 MtrunA17_Chr7g0261851 30.285 492 302 9 30 493 87 565 1.28e-60 209
Msa0163010 MtrunA17_Chr7g0240151 30.023 433 269 9 70 487 19 432 2.58e-59 202
Msa0163010 MtrunA17_Chr1g0166711 29.231 520 328 12 1 493 55 561 3.32e-58 202
Msa0163010 MtrunA17_Chr7g0217461 29.638 469 294 12 32 471 85 546 5.64e-57 199
Msa0163010 MtrunA17_Chr4g0069581 30.353 481 307 13 30 493 45 514 3.41e-55 194
Msa0163010 MtrunA17_Chr5g0415961 28.049 492 301 12 38 492 91 566 1.66e-54 192
Msa0163010 MtrunA17_Chr7g0261661 29.343 426 275 8 88 498 41 455 6.36e-54 189
Msa0163010 MtrunA17_Chr4g0011041 27.218 496 327 15 25 493 90 578 1.43e-53 190
Msa0163010 MtrunA17_Chr8g0362711 28.543 494 295 15 38 492 91 565 6.72e-52 185
Msa0163010 MtrunA17_Chr6g0451841 29.481 424 245 13 104 496 137 537 1.40e-51 183
Msa0163010 MtrunA17_Chr1g0166731 28.416 461 294 11 58 493 9 458 3.55e-48 173
Msa0163010 MtrunA17_Chr3g0112631 33.942 274 163 6 231 493 3 269 2.09e-47 166
Msa0163010 MtrunA17_Chr3g0142121 33.942 274 169 6 232 503 11 274 3.87e-43 154
Msa0163010 MtrunA17_Chr4g0060071 32.971 276 160 8 227 492 32 292 1.37e-39 145
Msa0163010 MtrunA17_Chr7g0261751 26.515 396 245 10 30 380 90 484 1.75e-35 139
Msa0163010 MtrunA17_Chr7g0234381 25.694 432 286 10 68 493 16 418 5.17e-32 127
Msa0163010 MtrunA17_Chr7g0234181 25.799 438 278 12 68 493 11 413 1.26e-30 123
Msa0163010 MtrunA17_Chr7g0261731 29.492 295 176 10 1 276 61 342 1.33e-30 122
Msa0163010 MtrunA17_Chr7g0234151 25.576 434 284 12 68 493 16 418 3.16e-28 116
Msa0163010 MtrunA17_Chr1g0177601 89.474 57 6 0 1 57 1 57 2.94e-27 103
Msa0163010 MtrunA17_Chr1g0177621 89.474 57 6 0 1 57 1 57 2.94e-27 103
Msa0163010 MtrunA17_Chr7g0261721 31.282 195 127 1 306 493 4 198 1.24e-26 107
Msa0163010 MtrunA17_Chr3g0142111 29.921 254 159 7 1 238 76 326 2.86e-26 109
Msa0163010 MtrunA17_Chr2g0326221 30.263 228 135 5 268 493 15 220 5.49e-26 105
Msa0163010 MtrunA17_Chr8g0382591 41.284 109 58 4 231 335 3 109 1.49e-17 79.3
Msa0163010 MtrunA17_Chr3g0112651 28.934 197 128 5 46 232 9 203 1.13e-14 73.2
Msa0163010 MtrunA17_Chr7g0261831 29.189 185 105 5 193 361 3 177 5.70e-12 65.1
Msa0163010 MtrunA17_Chr1g0147581 33.333 111 60 2 367 469 11 115 7.25e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163010 AT1G52190.1 50.857 525 233 7 1 500 49 573 0.0 548
Msa0163010 AT3G16180.1 51.711 526 230 10 1 503 51 575 0.0 541
Msa0163010 AT1G69870.1 34.594 529 316 11 2 503 80 605 5.27e-105 327
Msa0163010 AT1G62200.1 39.792 480 260 12 31 489 110 581 1.18e-103 322
Msa0163010 AT5G11570.1 38.507 509 226 12 1 494 41 477 1.06e-101 314
Msa0163010 AT2G02040.1 36.293 518 297 14 1 493 69 578 1.35e-99 311
Msa0163010 AT1G62200.2 38.264 507 259 13 31 489 110 610 1.16e-98 310
Msa0163010 AT1G62200.3 38.264 507 259 13 31 489 124 624 1.87e-98 310
Msa0163010 AT3G54140.1 36.253 491 287 11 24 493 75 560 1.27e-96 303
Msa0163010 AT3G54140.2 36.253 491 287 11 24 493 75 560 1.27e-96 303
Msa0163010 AT1G27080.2 32.634 524 324 12 2 503 1 517 9.58e-96 300
Msa0163010 AT5G62680.1 34.709 533 312 11 1 501 71 599 2.24e-95 301
Msa0163010 AT1G27080.1 32.634 524 324 12 2 503 43 559 4.17e-95 300
Msa0163010 AT1G18880.1 33.141 519 319 8 1 493 47 563 5.21e-95 300
Msa0163010 AT1G68570.2 34.151 530 319 10 1 503 21 547 2.12e-93 295
Msa0163010 AT1G68570.1 34.151 530 319 10 1 503 51 577 5.13e-93 295
Msa0163010 AT5G01180.2 35.294 476 285 8 31 487 83 554 4.28e-92 292
Msa0163010 AT5G01180.3 35.294 476 285 8 31 487 83 554 4.28e-92 292
Msa0163010 AT5G01180.1 35.294 476 285 8 31 487 83 554 4.28e-92 292
Msa0163010 AT3G47960.1 33.908 522 311 11 1 493 89 605 1.37e-90 290
Msa0163010 AT5G28470.2 33.054 478 294 12 30 486 74 546 4.67e-83 268
Msa0163010 AT2G02020.1 34.511 481 257 13 31 493 100 540 8.86e-83 267
Msa0163010 AT3G45650.1 32.812 512 304 11 1 493 50 540 1.12e-80 261
Msa0163010 AT5G28470.1 32.845 478 293 13 30 486 74 544 5.31e-80 260
Msa0163010 AT3G45650.2 31.227 538 304 12 1 493 50 566 4.39e-75 248
Msa0163010 AT3G54450.1 30.913 482 298 8 27 487 66 533 2.41e-74 245
Msa0163010 AT3G45710.1 30.980 510 318 11 1 493 49 541 3.35e-74 244
Msa0163010 AT1G72125.1 31.836 512 315 10 1 493 57 553 8.73e-74 244
Msa0163010 AT2G26690.3 32.157 510 327 10 1 495 7 512 1.22e-73 243
Msa0163010 AT2G26690.2 32.157 510 327 10 1 495 19 524 2.15e-73 242
Msa0163010 AT3G45660.1 30.994 513 312 12 1 493 49 539 2.33e-73 242
Msa0163010 AT3G45660.3 30.994 513 312 12 1 493 49 539 2.33e-73 242
Msa0163010 AT2G26690.1 32.157 510 327 10 1 495 52 557 4.16e-73 242
Msa0163010 AT3G45660.2 30.994 513 312 12 1 493 70 560 4.82e-73 242
Msa0163010 AT3G45720.1 30.845 509 318 12 1 493 47 537 4.65e-72 239
Msa0163010 AT2G37900.2 30.290 482 311 13 24 490 10 481 4.79e-72 237
Msa0163010 AT2G37900.1 30.290 482 311 13 24 490 89 560 2.97e-71 237
Msa0163010 AT3G53960.1 30.550 491 312 13 24 493 88 570 5.59e-71 237
Msa0163010 AT3G45680.1 32.417 509 311 12 1 493 48 539 2.66e-70 234
Msa0163010 AT5G13400.1 30.057 529 338 7 1 503 96 618 9.77e-70 234
Msa0163010 AT3G45700.1 30.784 510 312 12 1 493 44 529 2.44e-69 231
Msa0163010 AT1G69860.1 32.347 507 307 14 10 493 53 546 2.98e-69 231
Msa0163010 AT1G72130.1 31.176 510 302 14 1 493 53 530 4.61e-69 231
Msa0163010 AT1G22540.1 31.323 514 310 11 1 493 58 549 1.70e-68 229
Msa0163010 AT1G32450.1 29.532 491 316 11 32 495 96 583 3.97e-68 230
Msa0163010 AT1G22570.2 31.200 500 299 11 1 474 21 501 3.34e-67 225
Msa0163010 AT1G22570.1 31.200 500 299 11 1 474 58 538 7.91e-67 225
Msa0163010 AT1G33440.1 31.723 476 292 10 39 493 104 567 1.42e-66 226
Msa0163010 AT1G22550.1 29.730 518 320 10 1 493 58 556 1.51e-66 224
Msa0163010 AT1G72140.1 30.344 524 296 13 5 493 62 551 2.43e-66 224
Msa0163010 AT1G59740.1 31.323 514 318 15 1 490 69 571 5.14e-66 224
Msa0163010 AT5G46050.1 30.720 472 289 10 31 476 85 544 1.24e-65 223
Msa0163010 AT1G12110.1 30.725 524 320 13 1 493 55 566 2.74e-65 222
Msa0163010 AT2G02020.2 31.875 480 250 15 31 493 100 519 2.87e-65 220
Msa0163010 AT1G72120.1 30.545 514 315 11 1 493 57 549 1.35e-63 216
Msa0163010 AT4G21680.1 28.425 489 318 12 32 493 87 570 2.06e-63 217
Msa0163010 AT5G46040.1 30.867 473 287 10 31 476 85 544 4.40e-63 216
Msa0163010 AT3G21670.1 29.109 505 330 10 1 488 57 550 4.57e-63 216
Msa0163010 AT5G14940.1 30.604 513 317 13 1 488 35 533 2.12e-61 211
Msa0163010 AT2G40460.1 28.685 502 322 10 24 503 75 562 2.28e-61 211
Msa0163010 AT1G27040.3 29.615 520 319 12 1 492 23 523 4.37e-60 207
Msa0163010 AT1G27040.1 29.615 520 319 12 1 492 55 555 1.26e-59 206
Msa0163010 AT1G27040.2 29.615 520 319 12 1 492 51 551 1.50e-59 206
Msa0163010 AT5G19640.1 30.103 485 299 15 32 495 118 583 3.28e-59 206
Msa0163010 AT5G46050.2 30.641 421 264 9 72 476 24 432 4.36e-58 200
Msa0163010 AT1G72130.2 31.776 428 250 11 75 487 6 406 1.05e-55 192
Msa0163010 AT1G72130.3 31.776 428 250 11 75 487 6 406 1.05e-55 192
Msa0163010 AT3G01350.1 28.000 525 332 14 1 491 35 547 2.37e-54 192
Msa0163010 AT5G62730.2 28.487 509 307 14 14 492 39 520 6.08e-52 184
Msa0163010 AT5G62730.1 28.487 509 307 14 14 492 98 579 1.13e-51 185
Msa0163010 AT3G25260.1 27.044 477 302 12 25 492 73 512 1.15e-51 184
Msa0163010 AT3G45690.1 28.487 509 290 16 1 493 46 496 3.59e-50 179
Msa0163010 AT1G69850.1 28.425 489 311 14 38 492 90 573 1.27e-48 177
Msa0163010 AT3G25280.1 25.251 499 331 12 4 492 52 518 3.57e-40 152
Msa0163010 AT2G38100.2 24.710 518 330 21 1 493 2 484 4.25e-24 105
Msa0163010 AT2G38100.1 24.660 515 334 20 1 493 18 500 5.25e-24 105
Msa0163010 AT2G38100.3 24.660 515 334 20 1 493 24 506 6.63e-24 105

Find 117 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGTTGCTGCTTCCCTTTC+TGG 0.040603 1_4:-40589833 Msa0163010:CDS
CTGTATAATAAAACTCATTT+TGG 0.135749 1_4:+40589876 None:intergenic
GAACAAAGCTTGTGTTATTA+AGG 0.192435 1_4:-40590398 Msa0163010:CDS
TCCAAGACCCTATTGTTATT+AGG 0.209799 1_4:+40590826 None:intergenic
GCTGCTTCCCTTTCTGGATT+AGG 0.220823 1_4:-40589827 Msa0163010:CDS
CCAAGACCCTATTGTTATTA+GGG 0.240592 1_4:+40590827 None:intergenic
CTAGTTTGCAGTTGGGCTTA+TGG 0.253488 1_4:-40589638 Msa0163010:CDS
CTAGATAATAGAGTATATTA+AGG 0.272271 1_4:+40589657 None:intergenic
AGGTTCTGCTGTCAGTTTCC+TGG 0.294557 1_4:-40591802 Msa0163010:intron
TTCTTTGCACTGATATTAAC+TGG 0.295292 1_4:+40590067 None:intergenic
GTATATATCCAAGATCATTT+TGG 0.295506 1_4:-40590739 Msa0163010:CDS
CGGAAGATAAGAGGAGTATT+TGG 0.301250 1_4:+40591901 None:intergenic
TTTGGTTGGAAAATTGGTTT+TGG 0.301389 1_4:-40590721 Msa0163010:CDS
AAATGCGAACGATGGAGTTT+TGG 0.301463 1_4:-40589969 Msa0163010:CDS
GCATGTTGTAGTAAGAATTA+AGG 0.309274 1_4:-40589337 Msa0163010:three_prime_UTR
TGCAAAACTAGCGAACAAAT+TGG 0.320251 1_4:+40590618 None:intergenic
TTGTTAGAGTTATTCCATTA+TGG 0.323550 1_4:-40590288 Msa0163010:CDS
AAGATAACCCTAATAACAAT+AGG 0.342050 1_4:-40590834 Msa0163010:CDS
ATATAGGTACGGTAATTATA+TGG 0.342184 1_4:-40590144 Msa0163010:CDS
ATCTACTGTGCATAGATTCC+AGG 0.353747 1_4:+40590333 None:intergenic
CCTCTTATCTTTGATGCTAA+AGG 0.364995 1_4:+40590094 None:intergenic
TTTAGTCTCATGTCAATTGC+AGG 0.365344 1_4:-40590907 Msa0163010:CDS
GCTTGCACCTCAACTTTGTC+TGG 0.381207 1_4:-40589930 Msa0163010:CDS
GTTTCATGTTCCATGGCATT+TGG 0.391537 1_4:-40590877 Msa0163010:CDS
AACCACAGGCATGAACTTGC+TGG 0.394025 1_4:+40591875 None:intergenic
CGATTCTTAAGTGTTGGATT+AGG 0.396315 1_4:-40591822 Msa0163010:CDS
ATCAAATACAATATCATGTT+GGG 0.404738 1_4:+40591957 None:intergenic
GCAAAGAAAAGAATGGGAAT+TGG 0.406768 1_4:-40590052 Msa0163010:CDS
GGACCTTTCAAGCTTCATCT+TGG 0.414516 1_4:-40591933 Msa0163010:CDS
CCCTAATAACAATAGGGTCT+TGG 0.418911 1_4:-40590827 Msa0163010:CDS
CCTTTAGCATCAAAGATAAG+AGG 0.419532 1_4:-40590094 Msa0163010:CDS
TCAAGATATGGAAGGCTTTG+TGG 0.419950 1_4:-40589574 Msa0163010:three_prime_UTR
CTATTATCTAGTTTGCAGTT+GGG 0.424374 1_4:-40589645 Msa0163010:CDS
CAACATGATATTGTATTTGA+TGG 0.424400 1_4:-40591955 Msa0163010:exon
TCTTTGAGCGTTATCAATAT+AGG 0.428181 1_4:-40590160 Msa0163010:CDS
CTCGGTCGATTCTTAAGTGT+TGG 0.428412 1_4:-40591828 Msa0163010:CDS
TTTGTTGCAGATTCATATCT+CGG 0.435403 1_4:-40591846 Msa0163010:CDS
TCAGAAGCAGTCAATTTCAT+AGG 0.436089 1_4:-40589899 Msa0163010:CDS
GCACCTCAACTTTGTCTGGC+TGG 0.437194 1_4:-40589926 Msa0163010:CDS
TCTATTATCTAGTTTGCAGT+TGG 0.444992 1_4:-40589646 Msa0163010:CDS
ATGATCCCACTAGACCATAA+TGG 0.450318 1_4:+40590274 None:intergenic
AATGAGGTTGACTCAAGATA+TGG 0.450892 1_4:-40589586 Msa0163010:three_prime_UTR
TGCTGGCTGCGGAAGATAAG+AGG 0.457100 1_4:+40591892 None:intergenic
TATCAGTGCAAAGAAAAGAA+TGG 0.458159 1_4:-40590059 Msa0163010:CDS
ATCACCAACATCATGGGAAA+TGG 0.460646 1_4:-40590947 Msa0163010:CDS
GGAGGTGATATGTCTGTTCA+AGG 0.462389 1_4:+40590204 None:intergenic
TTCCCTTTCTGGATTAGGAA+TGG 0.462743 1_4:-40589822 Msa0163010:CDS
ATCAGTGCAAAGAAAAGAAT+GGG 0.469148 1_4:-40590058 Msa0163010:CDS
TTGTCACAATTGAGTAGGCT+TGG 0.469392 1_4:-40589417 Msa0163010:three_prime_UTR
AGATAACCCTAATAACAATA+GGG 0.471415 1_4:-40590833 Msa0163010:CDS
CGCTGGAACTTCAAAGTTGG+AGG 0.472600 1_4:+40590186 None:intergenic
ACCTACCTTAGTTTATCAGT+TGG 0.486210 1_4:+40590496 None:intergenic
TAGATAATAGAGTATATTAA+GGG 0.487308 1_4:+40589658 None:intergenic
ACTTCAAGAGGAGGAAAAGA+TGG 0.489744 1_4:-40589749 Msa0163010:CDS
GCGATCAATCACCAACATCA+TGG 0.496498 1_4:-40590954 Msa0163010:CDS
TGCAGTTGAAGAGTTAACTA+AGG 0.499479 1_4:-40589612 Msa0163010:CDS
TGCGGGTGTTTCATGTTCCA+TGG 0.499735 1_4:-40590884 Msa0163010:CDS
AAGTACTCTTGGCTTATAGC+TGG 0.504830 1_4:-40589689 Msa0163010:CDS
TTAGTTAACTCTTCAACTGC+AGG 0.507601 1_4:+40589614 None:intergenic
CTTGGCAATACTGCAAGCTT+AGG 0.510734 1_4:-40591987 Msa0163010:five_prime_UTR
AATTGATAATTTAAGATACT+CGG 0.510889 1_4:+40589271 None:intergenic
AGGCATGAACTTGCTGGCTG+CGG 0.511026 1_4:+40591881 None:intergenic
ATGATGACCCTTAGCATTGG+AGG 0.512129 1_4:-40590253 Msa0163010:CDS
TCCCGCTGGAACTTCAAAGT+TGG 0.516812 1_4:+40590183 None:intergenic
CATCAAATACAATATCATGT+TGG 0.523970 1_4:+40591956 None:intergenic
GCAACACTAGACATAGTACT+TGG 0.530889 1_4:+40589848 None:intergenic
AACATGATATTGTATTTGAT+GGG 0.530924 1_4:-40591954 Msa0163010:CDS
TCTCCAAGATGAAGCTTGAA+AGG 0.532522 1_4:+40591930 None:intergenic
GTTATTCCATTATGGTCTAG+TGG 0.537272 1_4:-40590280 Msa0163010:CDS
CTCATGTCAATTGCAGGTGC+GGG 0.540593 1_4:-40590901 Msa0163010:CDS
CGATCAATCACCAACATCAT+GGG 0.540764 1_4:-40590953 Msa0163010:CDS
AAAATGAGCCTCCAATGCTA+AGG 0.543292 1_4:+40590245 None:intergenic
ATGGTTCAAGAATAAACCCC+TGG 0.543754 1_4:-40590351 Msa0163010:CDS
GTGATTGTTTGCAGCAAATG+AGG 0.544320 1_4:-40592012 Msa0163010:five_prime_UTR
TCTCATGTCAATTGCAGGTG+CGG 0.545222 1_4:-40590902 Msa0163010:CDS
CAGCCATTCCTAATCCAGAA+AGG 0.545428 1_4:+40589819 None:intergenic
CTTTAGCATCAAAGATAAGA+GGG 0.548696 1_4:-40590093 Msa0163010:CDS
TGCAATTGACATGAGACTAA+AGG 0.550395 1_4:+40590909 None:intergenic
TGGTTCCAACTGATAAACTA+AGG 0.551260 1_4:-40590501 Msa0163010:intron
TCTGGATTAGGAATGGCTGC+AGG 0.559407 1_4:-40589815 Msa0163010:CDS
TAATGGAATAACTCTAACAA+TGG 0.561428 1_4:+40590291 None:intergenic
TTTGCAGCAAATGAGGCACT+TGG 0.561736 1_4:-40592005 Msa0163010:five_prime_UTR
ATCATGATGACCCTTAGCAT+TGG 0.562857 1_4:-40590256 Msa0163010:CDS
TTATTCCATTATGGTCTAGT+GGG 0.564458 1_4:-40590279 Msa0163010:CDS
TCTACTGTGCATAGATTCCA+GGG 0.566589 1_4:+40590334 None:intergenic
AGCCATTCCTAATCCAGAAA+GGG 0.568017 1_4:+40589820 None:intergenic
GGTGATTGATCGCCTTCCGT+TGG 0.568053 1_4:+40590964 None:intergenic
GAGTTAACTAAGGATTAATG+AGG 0.568152 1_4:-40589602 Msa0163010:exon
TTGTTGTGATGGTAAAATTG+AGG 0.569745 1_4:+40590538 None:intergenic
GAACAAGATATAGCATGTGA+TGG 0.572383 1_4:-40590370 Msa0163010:CDS
AGCCAGCAAGTTCATGCCTG+TGG 0.576101 1_4:-40591877 Msa0163010:CDS
AGCAAGTTCATGCCTGTGGT+TGG 0.578750 1_4:-40591873 Msa0163010:CDS
GAGCGTTATCAATATAGGTA+CGG 0.578923 1_4:-40590155 Msa0163010:CDS
TCCAACTTTGAAGTTCCAGC+GGG 0.578991 1_4:-40590184 Msa0163010:CDS
GATTTCTGATGATATTAACA+AGG 0.586027 1_4:-40589723 Msa0163010:CDS
TTGGTCTGCACCAAATGCCA+TGG 0.588107 1_4:+40590867 None:intergenic
ATTGCCATTTCCCATGATGT+TGG 0.593954 1_4:+40590943 None:intergenic
GATTTGAGTCCTTGTTGTGA+TGG 0.596972 1_4:+40590527 None:intergenic
TACAGGGAATGGCATTGTTG+TGG 0.598433 1_4:-40591005 Msa0163010:intron
GTAGAAAACACTACTTCAAG+AGG 0.601842 1_4:-40589761 Msa0163010:CDS
CTTCAAGAGGAGGAAAAGAT+GGG 0.604855 1_4:-40589748 Msa0163010:CDS
ATAACGCTCAAAGATCCCGC+TGG 0.608804 1_4:+40590169 None:intergenic
AGGAGAAGAAATGCGAACGA+TGG 0.609047 1_4:-40589977 Msa0163010:CDS
GCAACAAAAGCACCAACCAC+AGG 0.611443 1_4:+40591861 None:intergenic
CTCCAACTTTGAAGTTCCAG+CGG 0.612248 1_4:-40590185 Msa0163010:CDS
AAATGAGCCTCCAATGCTAA+GGG 0.618035 1_4:+40590246 None:intergenic
GAAAACACTACTTCAAGAGG+AGG 0.636406 1_4:-40589758 Msa0163010:CDS
AACAGCAATGATCAAACCAA+CGG 0.645820 1_4:-40590980 Msa0163010:CDS
GCAATGATCAAACCAACGGA+AGG 0.652956 1_4:-40590976 Msa0163010:CDS
ATTTCTGATGATATTAACAA+GGG 0.659432 1_4:-40589722 Msa0163010:CDS
CAACAAGGACTCAAATCTCG+TGG 0.669262 1_4:-40590521 Msa0163010:CDS
CAAGAGGAGGAAAAGATGGG+TGG 0.679323 1_4:-40589745 Msa0163010:CDS
CTACTGTGCATAGATTCCAG+GGG 0.694955 1_4:+40590335 None:intergenic
AGGTTGACTCAAGATATGGA+AGG 0.696704 1_4:-40589582 Msa0163010:three_prime_UTR
ATACCAGCCAGACAAAGTTG+AGG 0.697765 1_4:+40589923 None:intergenic
AGCAAGAAAGAATAACAGTG+TGG 0.706784 1_4:+40590672 None:intergenic
ATGCTAAAGGAATAAGAACG+CGG 0.757544 1_4:+40590107 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTAGTTTATATTTTT+GGG - chr1_4:40590018-40590037 Msa0163010:CDS 10.0%
!!! TCTATATAATTTTTGAATCA+TGG - chr1_4:40589489-40589508 Msa0163010:three_prime_UTR 15.0%
!! TTCATAAATAAATAGTGTAT+TGG + chr1_4:40589694-40589713 None:intergenic 15.0%
!!! TGATTTTCTCAAATTATTAA+TGG + chr1_4:40589770-40589789 None:intergenic 15.0%
!!! GAAGTTTTAGTTTATATTTT+TGG - chr1_4:40590017-40590036 Msa0163010:CDS 15.0%
!!! TTTTTAATCTTTACATAAAG+AGG + chr1_4:40590621-40590640 None:intergenic 15.0%
!!! ATAAATTTTGGTGAAATATT+CGG - chr1_4:40590817-40590836 Msa0163010:CDS 15.0%
!! TAGATAATAGAGTATATTAA+GGG + chr1_4:40591609-40591628 None:intergenic 15.0%
!!! AATTGATAATTTAAGATACT+CGG + chr1_4:40591996-40592015 None:intergenic 15.0%
!! ATCAAATACAATATCATGTT+GGG + chr1_4:40589310-40589329 None:intergenic 20.0%
!! AACATGATATTGTATTTGAT+GGG - chr1_4:40589310-40589329 Msa0163010:three_prime_UTR 20.0%
!! AAAAATTATATAGACATACC+AGG + chr1_4:40589483-40589502 None:intergenic 20.0%
!! TTTAGTGTAATTATATGTGT+CGG - chr1_4:40589795-40589814 Msa0163010:CDS 20.0%
!! TTATCCAATGAAAAAACTTA+TGG + chr1_4:40589973-40589992 None:intergenic 20.0%
!!! GGTTATCTTTTATATTTACT+TGG + chr1_4:40590419-40590438 None:intergenic 20.0%
!!! TAAAATTGAGGTGAATTTTT+AGG + chr1_4:40590717-40590736 None:intergenic 20.0%
!!! TTTGCATATTCCATAAATTT+TGG - chr1_4:40590805-40590824 Msa0163010:CDS 20.0%
!! GAATATTTCACCAAAATTTA+TGG + chr1_4:40590818-40590837 None:intergenic 20.0%
!!! CTGTATAATAAAACTCATTT+TGG + chr1_4:40591391-40591410 None:intergenic 20.0%
!! ATTTCTGATGATATTAACAA+GGG - chr1_4:40591542-40591561 Msa0163010:intron 20.0%
!! CTAGATAATAGAGTATATTA+AGG + chr1_4:40591610-40591629 None:intergenic 20.0%
!! ATACTGCAAAAATTCTTAAT+AGG - chr1_4:40591780-40591799 Msa0163010:intron 20.0%
! CATCAAATACAATATCATGT+TGG + chr1_4:40589311-40589330 None:intergenic 25.0%
! CAACATGATATTGTATTTGA+TGG - chr1_4:40589309-40589328 Msa0163010:three_prime_UTR 25.0%
!!! CTTGGTTTTAAATTACAGTT+GGG - chr1_4:40589566-40589585 Msa0163010:three_prime_UTR 25.0%
!!! TTGGTTTTAAATTACAGTTG+GGG - chr1_4:40589567-40589586 Msa0163010:three_prime_UTR 25.0%
! CACTATTTATTTATGAAGCA+CGG - chr1_4:40589697-40589716 Msa0163010:CDS 25.0%
!!! CTAACCATAAGTTTTTTCAT+TGG - chr1_4:40589966-40589985 Msa0163010:CDS 25.0%
!! CAACTTTTCTCATTAAATTG+CGG + chr1_4:40590208-40590227 None:intergenic 25.0%
! TGTAACAACAAAAAAGGATA+AGG + chr1_4:40590243-40590262 None:intergenic 25.0%
!!! TTATCCTTTTTTGTTGTTAC+AGG - chr1_4:40590242-40590261 Msa0163010:CDS 25.0%
!!! TATCCTTTTTTGTTGTTACA+GGG - chr1_4:40590243-40590262 Msa0163010:CDS 25.0%
! AAGATAACCCTAATAACAAT+AGG - chr1_4:40590430-40590449 Msa0163010:intron 25.0%
! AGATAACCCTAATAACAATA+GGG - chr1_4:40590431-40590450 Msa0163010:intron 25.0%
!! GTATATATCCAAGATCATTT+TGG - chr1_4:40590525-40590544 Msa0163010:CDS 25.0%
! TAATGGAATAACTCTAACAA+TGG + chr1_4:40590976-40590995 None:intergenic 25.0%
! TTGTTAGAGTTATTCCATTA+TGG - chr1_4:40590976-40590995 Msa0163010:CDS 25.0%
! ATATAGGTACGGTAATTATA+TGG - chr1_4:40591120-40591139 Msa0163010:intron 25.0%
!! GTTTTTATCTTTTCTCTACT+CGG - chr1_4:40591238-40591257 Msa0163010:intron 25.0%
! GATTTCTGATGATATTAACA+AGG - chr1_4:40591541-40591560 Msa0163010:intron 25.0%
! ATGAAGTCTAAAAAATTGTG+TGG - chr1_4:40591724-40591743 Msa0163010:intron 25.0%
! AATTGTGACAAAAACGAATA+TGG + chr1_4:40591838-40591857 None:intergenic 25.0%
! CAGAAAAAGAGAATTCATAT+GGG - chr1_4:40591878-40591897 Msa0163010:CDS 25.0%
TTTGTTGCAGATTCATATCT+CGG - chr1_4:40589418-40589437 Msa0163010:three_prime_UTR 30.0%
TCAATCAATACTTTGAACCT+TGG - chr1_4:40589548-40589567 Msa0163010:three_prime_UTR 30.0%
! CCAACTGTAATTTAAAACCA+AGG + chr1_4:40589568-40589587 None:intergenic 30.0%
!!! CCTTGGTTTTAAATTACAGT+TGG - chr1_4:40589565-40589584 Msa0163010:three_prime_UTR 30.0%
! TTTTAAACTATTAAACGCCG+CGG + chr1_4:40589640-40589659 None:intergenic 30.0%
ACAAACCATATAAAGTGACA+CGG - chr1_4:40589665-40589684 Msa0163010:CDS 30.0%
TCTTGGTTTACCTTGTAAAT+AGG - chr1_4:40589906-40589925 Msa0163010:CDS 30.0%
CTTGGTTTACCTTGTAAATA+GGG - chr1_4:40589907-40589926 Msa0163010:CDS 30.0%
ATAAGTATCACACAATTTCG+AGG + chr1_4:40590063-40590082 None:intergenic 30.0%
CAATATTTCAACCACAATTG+TGG - chr1_4:40590175-40590194 Msa0163010:CDS 30.0%
CGCAATTTAATGAGAAAAGT+TGG - chr1_4:40590206-40590225 Msa0163010:CDS 30.0%
ATTCCCTGTAACAACAAAAA+AGG + chr1_4:40590249-40590268 None:intergenic 30.0%
!!! TTTTTTGTTGTTACAGGGAA+TGG - chr1_4:40590248-40590267 Msa0163010:CDS 30.0%
!! TCTTGGAAATGTTTTTCAAC+TGG - chr1_4:40590454-40590473 Msa0163010:intron 30.0%
!! ATATCCAAGATCATTTTGGT+TGG - chr1_4:40590529-40590548 Msa0163010:CDS 30.0%
! TTTTCCAACCAAAATGATCT+TGG + chr1_4:40590536-40590555 None:intergenic 30.0%
!!! GATCATTTTGGTTGGAAAAT+TGG - chr1_4:40590537-40590556 Msa0163010:CDS 30.0%
!! TTTGGTTGGAAAATTGGTTT+TGG - chr1_4:40590543-40590562 Msa0163010:CDS 30.0%
! TGGAAGTTTTCTGTTGTTAT+AGG + chr1_4:40590688-40590707 None:intergenic 30.0%
!! AATTGAGGTGAATTTTTAGG+CGG + chr1_4:40590714-40590733 None:intergenic 30.0%
TTGTTGTGATGGTAAAATTG+AGG + chr1_4:40590729-40590748 None:intergenic 30.0%
! TCAATTTTACCATCACAACA+AGG - chr1_4:40590728-40590747 Msa0163010:CDS 30.0%
! GGAACTAACTTTTATATCTC+AGG - chr1_4:40590838-40590857 Msa0163010:CDS 30.0%
! GAACAAAGCTTGTGTTATTA+AGG - chr1_4:40590866-40590885 Msa0163010:CDS 30.0%
TTATTCCATTATGGTCTAGT+GGG - chr1_4:40590985-40591004 Msa0163010:CDS 30.0%
TCTTTGAGCGTTATCAATAT+AGG - chr1_4:40591104-40591123 Msa0163010:intron 30.0%
CTTTAGCATCAAAGATAAGA+GGG - chr1_4:40591171-40591190 Msa0163010:intron 30.0%
! TTCTTTGCACTGATATTAAC+TGG + chr1_4:40591200-40591219 None:intergenic 30.0%
TATCAGTGCAAAGAAAAGAA+TGG - chr1_4:40591205-40591224 Msa0163010:intron 30.0%
ATCAGTGCAAAGAAAAGAAT+GGG - chr1_4:40591206-40591225 Msa0163010:intron 30.0%
! TCTATTATCTAGTTTGCAGT+TGG - chr1_4:40591618-40591637 Msa0163010:intron 30.0%
! CTATTATCTAGTTTGCAGTT+GGG - chr1_4:40591619-40591638 Msa0163010:intron 30.0%
GAGTTAACTAAGGATTAATG+AGG - chr1_4:40591662-40591681 Msa0163010:intron 30.0%
! CCATATGAATTCTCTTTTTC+TGG + chr1_4:40591880-40591899 None:intergenic 30.0%
CCAGAAAAAGAGAATTCATA+TGG - chr1_4:40591877-40591896 Msa0163010:CDS 30.0%
GCATGTTGTAGTAAGAATTA+AGG - chr1_4:40591927-40591946 Msa0163010:CDS 30.0%
!! CGATTCTTAAGTGTTGGATT+AGG - chr1_4:40589442-40589461 Msa0163010:three_prime_UTR 35.0%
CAAACCATATAAAGTGACAC+GGG - chr1_4:40589666-40589685 Msa0163010:CDS 35.0%
TAAACCAAGATTGCTTTGGT+GGG + chr1_4:40589896-40589915 None:intergenic 35.0%
AAGGTAAACCAAGATTGCTT+TGG + chr1_4:40589900-40589919 None:intergenic 35.0%
TACCTTGTAAATAGGGATTG+AGG - chr1_4:40589914-40589933 Msa0163010:CDS 35.0%
ACCTTGTAAATAGGGATTGA+GGG - chr1_4:40589915-40589934 Msa0163010:CDS 35.0%
TACTTATCCTGATGCAGTTA+CGG - chr1_4:40590076-40590095 Msa0163010:CDS 35.0%
AACAGCAATGATCAAACCAA+CGG - chr1_4:40590284-40590303 Msa0163010:CDS 35.0%
TGCAATTGACATGAGACTAA+AGG + chr1_4:40590358-40590377 None:intergenic 35.0%
TTTAGTCTCATGTCAATTGC+AGG - chr1_4:40590357-40590376 Msa0163010:CDS 35.0%
! CCAAGACCCTATTGTTATTA+GGG + chr1_4:40590440-40590459 None:intergenic 35.0%
! TCCAAGACCCTATTGTTATT+AGG + chr1_4:40590441-40590460 None:intergenic 35.0%
! GATAAAAACATGAGTGCTGT+TGG + chr1_4:40590573-40590592 None:intergenic 35.0%
AGCAAGAAAGAATAACAGTG+TGG + chr1_4:40590595-40590614 None:intergenic 35.0%
TGCAAAACTAGCGAACAAAT+TGG + chr1_4:40590649-40590668 None:intergenic 35.0%
TGGTTCCAACTGATAAACTA+AGG - chr1_4:40590763-40590782 Msa0163010:CDS 35.0%
TCCAACTGATAAACTAAGGT+AGG - chr1_4:40590767-40590786 Msa0163010:CDS 35.0%
ACCTACCTTAGTTTATCAGT+TGG + chr1_4:40590771-40590790 None:intergenic 35.0%
GAACAAGATATAGCATGTGA+TGG - chr1_4:40590894-40590913 Msa0163010:CDS 35.0%
GTTATTCCATTATGGTCTAG+TGG - chr1_4:40590984-40591003 Msa0163010:CDS 35.0%
GAGCGTTATCAATATAGGTA+CGG - chr1_4:40591109-40591128 Msa0163010:intron 35.0%
ATGCTAAAGGAATAAGAACG+CGG + chr1_4:40591160-40591179 None:intergenic 35.0%
CCTCTTATCTTTGATGCTAA+AGG + chr1_4:40591173-40591192 None:intergenic 35.0%
CCTTTAGCATCAAAGATAAG+AGG - chr1_4:40591170-40591189 Msa0163010:intron 35.0%
GCAAAGAAAAGAATGGGAAT+TGG - chr1_4:40591212-40591231 Msa0163010:intron 35.0%
!!! CAGCGATTTTTGAGACTATA+AGG - chr1_4:40591267-40591286 Msa0163010:intron 35.0%
TCAGAAGCAGTCAATTTCAT+AGG - chr1_4:40591365-40591384 Msa0163010:intron 35.0%
GTAGAAAACACTACTTCAAG+AGG - chr1_4:40591503-40591522 Msa0163010:intron 35.0%
!! GTCATTTTGACAAGTACTCT+TGG - chr1_4:40591564-40591583 Msa0163010:intron 35.0%
TTAGTTAACTCTTCAACTGC+AGG + chr1_4:40591653-40591672 None:intergenic 35.0%
TGCAGTTGAAGAGTTAACTA+AGG - chr1_4:40591652-40591671 Msa0163010:intron 35.0%
! AATGAGGTTGACTCAAGATA+TGG - chr1_4:40591678-40591697 Msa0163010:intron 35.0%
! CGTTTTTGTCACAATTGAGT+AGG - chr1_4:40591842-40591861 Msa0163010:CDS 35.0%
GTGATTGTTTGCAGCAAATG+AGG - chr1_4:40589252-40589271 Msa0163010:three_prime_UTR 40.0%
TCTCCAAGATGAAGCTTGAA+AGG + chr1_4:40589337-40589356 None:intergenic 40.0%
!! CGGAAGATAAGAGGAGTATT+TGG + chr1_4:40589366-40589385 None:intergenic 40.0%
CGTTGCGATTGTGATCATTA+CGG - chr1_4:40589591-40589610 Msa0163010:exon 40.0%
GTAAACCAAGATTGCTTTGG+TGG + chr1_4:40589897-40589916 None:intergenic 40.0%
CCTTGTAAATAGGGATTGAG+GGG - chr1_4:40589916-40589935 Msa0163010:CDS 40.0%
CGATCAATCACCAACATCAT+GGG - chr1_4:40590311-40590330 Msa0163010:CDS 40.0%
ATCACCAACATCATGGGAAA+TGG - chr1_4:40590317-40590336 Msa0163010:CDS 40.0%
! ATTGCCATTTCCCATGATGT+TGG + chr1_4:40590324-40590343 None:intergenic 40.0%
GTTTCATGTTCCATGGCATT+TGG - chr1_4:40590387-40590406 Msa0163010:CDS 40.0%
CCCTAATAACAATAGGGTCT+TGG - chr1_4:40590437-40590456 Msa0163010:intron 40.0%
!! GGTGAATTTTTAGGCGGTAA+TGG + chr1_4:40590708-40590727 None:intergenic 40.0%
! GATTTGAGTCCTTGTTGTGA+TGG + chr1_4:40590740-40590759 None:intergenic 40.0%
ATGGTTCAAGAATAAACCCC+TGG - chr1_4:40590913-40590932 Msa0163010:CDS 40.0%
TCTACTGTGCATAGATTCCA+GGG + chr1_4:40590933-40590952 None:intergenic 40.0%
ATCTACTGTGCATAGATTCC+AGG + chr1_4:40590934-40590953 None:intergenic 40.0%
ATGATCCCACTAGACCATAA+TGG + chr1_4:40590993-40591012 None:intergenic 40.0%
ATCATGATGACCCTTAGCAT+TGG - chr1_4:40591008-40591027 Msa0163010:intron 40.0%
AAATGAGCCTCCAATGCTAA+GGG + chr1_4:40591021-40591040 None:intergenic 40.0%
AAAATGAGCCTCCAATGCTA+AGG + chr1_4:40591022-40591041 None:intergenic 40.0%
! AAATGCGAACGATGGAGTTT+TGG - chr1_4:40591295-40591314 Msa0163010:intron 40.0%
!!! CGATGGAGTTTTGGAAATGT+CGG - chr1_4:40591304-40591323 Msa0163010:intron 40.0%
!! TTTTGGAAATGTCGGCATTG+TGG - chr1_4:40591312-40591331 Msa0163010:intron 40.0%
GCAACACTAGACATAGTACT+TGG + chr1_4:40591419-40591438 None:intergenic 40.0%
! TTCCCTTTCTGGATTAGGAA+TGG - chr1_4:40591442-40591461 Msa0163010:intron 40.0%
AGCCATTCCTAATCCAGAAA+GGG + chr1_4:40591447-40591466 None:intergenic 40.0%
GAAAACACTACTTCAAGAGG+AGG - chr1_4:40591506-40591525 Msa0163010:intron 40.0%
ACTTCAAGAGGAGGAAAAGA+TGG - chr1_4:40591515-40591534 Msa0163010:intron 40.0%
CTTCAAGAGGAGGAAAAGAT+GGG - chr1_4:40591516-40591535 Msa0163010:intron 40.0%
AAGTACTCTTGGCTTATAGC+TGG - chr1_4:40591575-40591594 Msa0163010:intron 40.0%
! AGGTTGACTCAAGATATGGA+AGG - chr1_4:40591682-40591701 Msa0163010:intron 40.0%
TCAAGATATGGAAGGCTTTG+TGG - chr1_4:40591690-40591709 Msa0163010:intron 40.0%
TTGTCACAATTGAGTAGGCT+TGG - chr1_4:40591847-40591866 Msa0163010:CDS 40.0%
! TTTGCAGCAAATGAGGCACT+TGG - chr1_4:40589259-40589278 Msa0163010:three_prime_UTR 45.0%
CTTGGCAATACTGCAAGCTT+AGG - chr1_4:40589277-40589296 Msa0163010:three_prime_UTR 45.0%
GGACCTTTCAAGCTTCATCT+TGG - chr1_4:40589331-40589350 Msa0163010:three_prime_UTR 45.0%
! CTCGGTCGATTCTTAAGTGT+TGG - chr1_4:40589436-40589455 Msa0163010:three_prime_UTR 45.0%
!! ATGTGTCGGTGTCGTGTTAT+CGG - chr1_4:40589809-40589828 Msa0163010:CDS 45.0%
TTTGCCCACCAAAGCAATCT+TGG - chr1_4:40589889-40589908 Msa0163010:CDS 45.0%
CCCCTCAATCCCTATTTACA+AGG + chr1_4:40589919-40589938 None:intergenic 45.0%
TAAAGTTCACACCGCAGTTG+CGG - chr1_4:40590121-40590140 Msa0163010:CDS 45.0%
TACAGGGAATGGCATTGTTG+TGG - chr1_4:40590259-40590278 Msa0163010:CDS 45.0%
GCAATGATCAAACCAACGGA+AGG - chr1_4:40590288-40590307 Msa0163010:CDS 45.0%
GCGATCAATCACCAACATCA+TGG - chr1_4:40590310-40590329 Msa0163010:CDS 45.0%
TCTCATGTCAATTGCAGGTG+CGG - chr1_4:40590362-40590381 Msa0163010:CDS 45.0%
CAACAAGGACTCAAATCTCG+TGG - chr1_4:40590743-40590762 Msa0163010:CDS 45.0%
CTACTGTGCATAGATTCCAG+GGG + chr1_4:40590932-40590951 None:intergenic 45.0%
! ATGATGACCCTTAGCATTGG+AGG - chr1_4:40591011-40591030 Msa0163010:intron 45.0%
GGAGGTGATATGTCTGTTCA+AGG + chr1_4:40591063-40591082 None:intergenic 45.0%
!! CTCCAACTTTGAAGTTCCAG+CGG - chr1_4:40591079-40591098 Msa0163010:intron 45.0%
!! TCCAACTTTGAAGTTCCAGC+GGG - chr1_4:40591080-40591099 Msa0163010:intron 45.0%
AGGAGAAGAAATGCGAACGA+TGG - chr1_4:40591287-40591306 Msa0163010:intron 45.0%
ATACCAGCCAGACAAAGTTG+AGG + chr1_4:40591344-40591363 None:intergenic 45.0%
CAGCCATTCCTAATCCAGAA+AGG + chr1_4:40591448-40591467 None:intergenic 45.0%
! CTAGTTTGCAGTTGGGCTTA+TGG - chr1_4:40591626-40591645 Msa0163010:intron 45.0%
AACCACAGGCATGAACTTGC+TGG + chr1_4:40589392-40589411 None:intergenic 50.0%
AGCAAGTTCATGCCTGTGGT+TGG - chr1_4:40589391-40589410 Msa0163010:three_prime_UTR 50.0%
!! GCAACAAAAGCACCAACCAC+AGG + chr1_4:40589406-40589425 None:intergenic 50.0%
AGGTTCTGCTGTCAGTTTCC+TGG - chr1_4:40589462-40589481 Msa0163010:three_prime_UTR 50.0%
!! GGCACCCGTGTCACTTTATA+TGG + chr1_4:40589673-40589692 None:intergenic 50.0%
GATTAGGCATGTCCCAGTGA+CGG + chr1_4:40589843-40589862 None:intergenic 50.0%
ACTGGGACATGCCTAATCTG+AGG - chr1_4:40589845-40589864 Msa0163010:CDS 50.0%
GCACGAACACTCCTCAGATT+AGG + chr1_4:40589859-40589878 None:intergenic 50.0%
ATCCTGATGCAGTTACGGTG+TGG - chr1_4:40590081-40590100 Msa0163010:CDS 50.0%
CTCATGTCAATTGCAGGTGC+GGG - chr1_4:40590363-40590382 Msa0163010:CDS 50.0%
TGCGGGTGTTTCATGTTCCA+TGG - chr1_4:40590380-40590399 Msa0163010:CDS 50.0%
TTGGTCTGCACCAAATGCCA+TGG + chr1_4:40590400-40590419 None:intergenic 50.0%
CGCTGGAACTTCAAAGTTGG+AGG + chr1_4:40591081-40591100 None:intergenic 50.0%
TCCCGCTGGAACTTCAAAGT+TGG + chr1_4:40591084-40591103 None:intergenic 50.0%
ATAACGCTCAAAGATCCCGC+TGG + chr1_4:40591098-40591117 None:intergenic 50.0%
GCTTGCACCTCAACTTTGTC+TGG - chr1_4:40591334-40591353 Msa0163010:intron 50.0%
! AGTGTTGCTGCTTCCCTTTC+TGG - chr1_4:40591431-40591450 Msa0163010:intron 50.0%
GCTGCTTCCCTTTCTGGATT+AGG - chr1_4:40591437-40591456 Msa0163010:intron 50.0%
! TCTGGATTAGGAATGGCTGC+AGG - chr1_4:40591449-40591468 Msa0163010:intron 50.0%
CAAGAGGAGGAAAAGATGGG+TGG - chr1_4:40591519-40591538 Msa0163010:intron 50.0%
! TGCTGGCTGCGGAAGATAAG+AGG + chr1_4:40589375-40589394 None:intergenic 55.0%
!! AGGCATGAACTTGCTGGCTG+CGG + chr1_4:40589386-40589405 None:intergenic 55.0%
AGCCAGCAAGTTCATGCCTG+TGG - chr1_4:40589387-40589406 Msa0163010:three_prime_UTR 55.0%
AGCCACACCGTAACTGCATC+AGG + chr1_4:40590086-40590105 None:intergenic 55.0%
ATGGCATCACACCGCAACTG+CGG + chr1_4:40590135-40590154 None:intergenic 55.0%
! TGATGCGCTAGCCTCTACGA+TGG + chr1_4:40590154-40590173 None:intergenic 55.0%
GCGGTCTGCATCCACAATTG+TGG + chr1_4:40590189-40590208 None:intergenic 55.0%
GGTGATTGATCGCCTTCCGT+TGG + chr1_4:40590303-40590322 None:intergenic 55.0%
GCACCTCAACTTTGTCTGGC+TGG - chr1_4:40591338-40591357 Msa0163010:intron 55.0%
CGACTGCATTGCAACTGCCG+CGG - chr1_4:40589620-40589639 Msa0163010:CDS 60.0%
ATCGGACACGTGTCCGTCAC+TGG - chr1_4:40589827-40589846 Msa0163010:CDS 60.0%
TCGGACACGTGTCCGTCACT+GGG - chr1_4:40589828-40589847 Msa0163010:CDS 60.0%
GCGGTGTGATGCCATCGTAG+AGG - chr1_4:40590140-40590159 Msa0163010:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 40589240 40592046 40589240 ID=Msa0163010;Name=Msa0163010
chr1_4 mRNA 40589240 40592046 40589240 ID=Msa0163010-mRNA-1;Parent=Msa0163010;Name=Msa0163010-mRNA-1;_AED=0.10;_eAED=0.10;_QI=73|1|1|1|1|1|3|367|503
chr1_4 exon 40589240 40590426 40589240 ID=Msa0163010-mRNA-1:exon:11745;Parent=Msa0163010-mRNA-1
chr1_4 exon 40590502 40591022 40590502 ID=Msa0163010-mRNA-1:exon:11744;Parent=Msa0163010-mRNA-1
chr1_4 exon 40591803 40592046 40591803 ID=Msa0163010-mRNA-1:exon:11743;Parent=Msa0163010-mRNA-1
chr1_4 five_prime_UTR 40591974 40592046 40591974 ID=Msa0163010-mRNA-1:five_prime_utr;Parent=Msa0163010-mRNA-1
chr1_4 CDS 40591803 40591973 40591803 ID=Msa0163010-mRNA-1:cds;Parent=Msa0163010-mRNA-1
chr1_4 CDS 40590502 40591022 40590502 ID=Msa0163010-mRNA-1:cds;Parent=Msa0163010-mRNA-1
chr1_4 CDS 40589607 40590426 40589607 ID=Msa0163010-mRNA-1:cds;Parent=Msa0163010-mRNA-1
chr1_4 three_prime_UTR 40589240 40589606 40589240 ID=Msa0163010-mRNA-1:three_prime_utr;Parent=Msa0163010-mRNA-1
Gene Sequence

>Msa0163010

ATGATATTGTATTTGATGGGACCTTTCAAGCTTCATCTTGGAGAAGCTAACCAAATACTCCTCTTATCTTCCGCAGCCAGCAAGTTCATGCCTGTGGTTGGTGCTTTTGTTGCAGATTCATATCTCGGTCGATTCTTAAGTGTTGGATTAGGTTCTGCTGTCAGTTTCCTGGGAATGGCATTGTTGTGGTTAACAGCAATGATCAAACCAACGGAAGGCGATCAATCACCAACATCATGGGAAATGGCAATGTTAATATCTGCCTTTAGTCTCATGTCAATTGCAGGTGCGGGTGTTTCATGTTCCATGGCATTTGGTGCAGACCAAGTAAATATAAAAGATAACCCTAATAACAATAGGGTCTTGGAAATGTTTTTCAACTGGTATTATGCTTTTGCAAGTATCTCTGCGATAATAGCTCTCACTGTAATAGTATATATCCAAGATCATTTTGGTTGGAAAATTGGTTTTGGAGTTCCAACAGCACTCATGTTTTTATCCACACTGTTATTCTTTCTTGCTTCTCCTCTTTATGTAAAGATTAAAAAAAGAACCAATTTGTTCGCTAGTTTTGCACAAGTAATCGTTGCTTCCTATAACAACAGAAAACTTCCATTACCGCCTAAAAATTCACCTCAATTTTACCATCACAACAAGGACTCAAATCTCGTGGTTCCAACTGATAAACTAAGGTTTCTGAACAAAGCTTGTGTTATTAAGGAATTTGAACAAGATATAGCATGTGATGGTTCAAGAATAAACCCCTGGAATCTATGCACAGTAGATCAAGTAGAAGAACTAAAAGCCATTGTTAGAGTTATTCCATTATGGTCTAGTGGGATCATGATGACCCTTAGCATTGGAGGCTCATTTTCATTGCTTCAAGCTAAAACCTTGAACAGACATATCACCTCCAACTTTGAAGTTCCAGCGGGATCTTTGAGCGTTATCAATATAGGTACGGTAATTATATGGATAGTTTTTTATGACCGCGTTCTTATTCCTTTAGCATCAAAGATAAGAGGGAAACCAGTTAATATCAGTGCAAAGAAAAGAATGGGAATTGGTTTGTTTTTATCTTTTCTCTACTCGGTAAATGCAGCGATTTTTGAGACTATAAGGAGAAGAAATGCGAACGATGGAGTTTTGGAAATGTCGGCATTGTGGCTTGCACCTCAACTTTGTCTGGCTGGTATATCAGAAGCAGTCAATTTCATAGGCCAAAATGAGTTTTATTATACAGAGTTTCCAAGTACTATGTCTAGTGTTGCTGCTTCCCTTTCTGGATTAGGAATGGCTGCAGGAAGCTTGTTATCTTCTTTATTGTTCAGCATTGTAGAAAACACTACTTCAAGAGGAGGAAAAGATGGGTGGATTTCTGATGATATTAACAAGGGTCATTTTGACAAGTACTCTTGGCTTATAGCTGGAGTTAGTGCCCTTAATATACTCTATTATCTAGTTTGCAGTTGGGCTTATGGACCTGCAGTTGAAGAGTTAACTAAGGATTAA
Protein sequence

>Msa0163010

MILYLMGPFKLHLGEANQILLLSSAASKFMPVVGAFVADSYLGRFLSVGLGSAVSFLGMALLWLTAMIKPTEGDQSPTSWEMAMLISAFSLMSIAGAGVSCSMAFGADQVNIKDNPNNNRVLEMFFNWYYAFASISAIIALTVIVYIQDHFGWKIGFGVPTALMFLSTLLFFLASPLYVKIKKRTNLFASFAQVIVASYNNRKLPLPPKNSPQFYHHNKDSNLVVPTDKLRFLNKACVIKEFEQDIACDGSRINPWNLCTVDQVEELKAIVRVIPLWSSGIMMTLSIGGSFSLLQAKTLNRHITSNFEVPAGSLSVINIGTVIIWIVFYDRVLIPLASKIRGKPVNISAKKRMGIGLFLSFLYSVNAAIFETIRRRNANDGVLEMSALWLAPQLCLAGISEAVNFIGQNEFYYTEFPSTMSSVAASLSGLGMAAGSLLSSLLFSIVENTTSRGGKDGWISDDINKGHFDKYSWLIAGVSALNILYYLVCSWAYGPAVEELTKD*