AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0270280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0270280 MtrunA17_Chr2g0277071 93.789 161 3 1 1 154 1 161 1.05e-108 306
Msa0270280 MtrunA17_Chr2g0281541 45.714 105 48 2 47 148 53 151 4.00e-24 92.0
Msa0270280 MtrunA17_Chr4g0047901 45.833 120 46 5 38 149 25 133 3.47e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0270280 AT1G09812.1 46.715 137 59 3 8 144 3 125 1.44e-29 105
Msa0270280 AT1G58007.4 48.571 105 44 3 42 146 20 114 1.64e-23 89.4
Msa0270280 AT1G58007.3 48.571 105 44 3 42 146 20 114 1.64e-23 89.4
Msa0270280 AT1G58007.2 48.571 105 44 3 42 146 20 114 1.64e-23 89.4
Msa0270280 AT1G11120.2 46.341 123 58 4 32 147 24 145 2.07e-22 87.8
Msa0270280 AT1G11120.1 39.597 149 58 5 31 147 23 171 2.27e-19 80.5
Msa0270280 AT4G28170.1 36.207 116 52 6 23 133 37 135 8.94e-12 59.7
Msa0270280 AT1G58007.1 81.250 32 6 0 42 73 20 51 1.78e-11 56.6

Find 68 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTATTCATGTGACTTAA+AGG 0.213889 2_3:-1554476 Msa0270280:five_prime_UTR
AAAAGTGACACTCATATTTA+AGG 0.221587 2_3:+1554408 None:intergenic
TAAAGAGACTAAGAGATTAT+TGG 0.226828 2_3:-1553329 Msa0270280:three_prime_UTR
GATTTCAATGTTTCTAATAA+TGG 0.253747 2_3:-1553761 Msa0270280:CDS
TTTGAACAAATGTTCAAATA+TGG 0.259962 2_3:+1553738 None:intergenic
GTCCAAGGGAAGAAGCTTAT+TGG 0.267181 2_3:-1553446 Msa0270280:CDS
GTGTGAAGCATTTAAGAAAT+TGG 0.309557 2_3:-1554152 Msa0270280:five_prime_UTR
TAGAAACATTGAAATCAAAA+TGG 0.315037 2_3:+1553768 None:intergenic
AAAGAGACTAAGAGATTATT+GGG 0.315819 2_3:-1553328 Msa0270280:three_prime_UTR
ATCTATGCTTCCAACTTATC+AGG 0.349676 2_3:-1553686 Msa0270280:CDS
TCTATGAGTGCAATATCATT+TGG 0.374376 2_3:-1553805 Msa0270280:CDS
GAAGGTCCATGGAAAAGCTT+TGG 0.389743 2_3:-1553832 Msa0270280:CDS
CATCATTTACTCATTGACTT+TGG 0.395198 2_3:+1553256 None:intergenic
GGCCAATAAGCTTCTTCCCT+TGG 0.399066 2_3:+1553444 None:intergenic
GTCTTATGCATGTGATTTGT+CGG 0.403359 2_3:-1553526 Msa0270280:CDS
ATTCATGTGACTTAAAGGTT+TGG 0.410583 2_3:-1554471 Msa0270280:five_prime_UTR
ATGTAAGCCATGTTTCCATT+TGG 0.438502 2_3:-1553286 Msa0270280:three_prime_UTR
TGATTTGTCGGTGTTGATGC+CGG 0.444835 2_3:-1553514 Msa0270280:CDS
TGTTAAATACAAAATGTTGT+TGG 0.457080 2_3:+1553415 None:intergenic
CTTGGACAAGGCAGAGGAGC+AGG 0.461138 2_3:+1553462 None:intergenic
ACAAGGCAGAGGAGCAGGTT+GGG 0.462248 2_3:+1553467 None:intergenic
GTAATAGGAATAGGAGTGGT+TGG 0.462562 2_3:-1554529 None:intergenic
GATTTGTCGGTGTTGATGCC+GGG 0.464814 2_3:-1553513 Msa0270280:CDS
AATTAGAACAAAGACAAAAC+AGG 0.469285 2_3:-1553972 Msa0270280:five_prime_UTR
TCAAGAAAACGTAATATGTC+AGG 0.477996 2_3:+1553146 None:intergenic
TCTTCCCTTGGACAAGGCAG+AGG 0.486050 2_3:+1553456 None:intergenic
AAGGTCCATGGAAAAGCTTT+GGG 0.488752 2_3:-1553831 Msa0270280:CDS
TAAGTTGGAAGCATAGATTC+TGG 0.501581 2_3:+1553691 None:intergenic
AGGAGGAGGAGGAGTTGATG+AGG 0.501669 2_3:-1553854 Msa0270280:CDS
CTGCTCCTCTGCCTTGTCCA+AGG 0.512022 2_3:-1553461 Msa0270280:CDS
CCTCCTGTCTGTGAATGTGA+TGG 0.513542 2_3:+1553871 None:intergenic
GACTTTGGAGAACAGCCAAA+TGG 0.513956 2_3:+1553271 None:intergenic
GATTTCATCTCATGTAGTCT+AGG 0.515564 2_3:+1554434 None:intergenic
ACAAAATGTTGTTGGTGAGA+AGG 0.517710 2_3:+1553423 None:intergenic
TTCTGCCTCTTGAGACAGCA+AGG 0.520372 2_3:-1554346 Msa0270280:five_prime_UTR
TGTGAGATCAGAATGAAGTT+TGG 0.528238 2_3:+1554125 None:intergenic
TGCTCCTCTGCCTTGTCCAA+GGG 0.530867 2_3:-1553460 Msa0270280:CDS
TAAGTAGGATATTGCTGCCC+CGG 0.537196 2_3:+1553495 None:intergenic
GACAAGGCAGAGGAGCAGGT+TGG 0.540635 2_3:+1553466 None:intergenic
GTATACACGGCTTCAAACGC+AGG 0.541704 2_3:-1553895 Msa0270280:CDS
AAGTCTTAACACTAAAATGA+AGG 0.550501 2_3:+1553171 None:intergenic
GAGTTGATGAGGAAGGTCCA+TGG 0.553113 2_3:-1553843 Msa0270280:CDS
GTGACACTCATATTTAAGGA+TGG 0.554314 2_3:+1554412 None:intergenic
CATTGAAATCAAAATGGCTG+TGG 0.558224 2_3:+1553774 None:intergenic
CATTCACAGACAGGAGGAGG+AGG 0.558241 2_3:-1553865 Msa0270280:CDS
GAGTGGTGTTCCTGATAAGT+TGG 0.558569 2_3:+1553676 None:intergenic
GCAGGTTGGGCAATGTAAGT+AGG 0.571068 2_3:+1553480 None:intergenic
ACATGAATAGTGTGCCTTGT+GGG 0.572826 2_3:+1554486 None:intergenic
TAAGTTTGCTTCATCTGACA+AGG 0.587342 2_3:-1554300 Msa0270280:intron
GGTCCATCACATTCACAGAC+AGG 0.587985 2_3:-1553874 Msa0270280:CDS
TCACATTCACAGACAGGAGG+AGG 0.591315 2_3:-1553868 Msa0270280:CDS
ATGGGCCTTGCTGTCTCAAG+AGG 0.592353 2_3:+1554341 None:intergenic
GGAGGAGGAGTTGATGAGGA+AGG 0.605051 2_3:-1553850 Msa0270280:CDS
TAAGCTTCTTCCCTTGGACA+AGG 0.606151 2_3:+1553450 None:intergenic
TCACTACCAACCACAAAACA+AGG 0.611869 2_3:-1553655 Msa0270280:CDS
AGTCAATGAGTAAATGATGA+CGG 0.613545 2_3:-1553252 Msa0270280:three_prime_UTR
GTGGAAGAAAAGTGTTGTGT+GGG 0.618638 2_3:+1553715 None:intergenic
AGAACAGCCAAATGGAAACA+TGG 0.620971 2_3:+1553279 None:intergenic
TTGCTTGCACTTGTCCCACA+AGG 0.622885 2_3:-1554500 Msa0270280:five_prime_UTR
TTATAAAGCTTAGCTAACTG+TGG 0.627301 2_3:-1554189 Msa0270280:intron
ACAGTGAAAGATCAAAGATG+TGG 0.629582 2_3:-1553921 Msa0270280:CDS
CGTGGAAGAAAAGTGTTGTG+TGG 0.639502 2_3:+1553714 None:intergenic
ATTTGTCGGTGTTGATGCCG+GGG 0.648111 2_3:-1553512 Msa0270280:CDS
AAAGATGTGGAACGTATACA+CGG 0.657288 2_3:-1553908 Msa0270280:CDS
TAATGTAATAGGAATAGGAG+TGG 0.674683 2_3:-1554533 None:intergenic
CACATGAATAGTGTGCCTTG+TGG 0.676976 2_3:+1554485 None:intergenic
TGGAAGCATAGATTCTGGCG+TGG 0.678051 2_3:+1553696 None:intergenic
CCATCACATTCACAGACAGG+AGG 0.694212 2_3:-1553871 Msa0270280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAAAAAAGAATAAT+GGG + chr2_3:1553350-1553369 None:intergenic 10.0%
!! AAAGAAATAAAAAAGAATAA+TGG + chr2_3:1553351-1553370 None:intergenic 10.0%
!! AAGAAATAAAAAAGAATAAT+GGG + chr2_3:1553350-1553369 None:intergenic 10.0%
!! AAAGAAATAAAAAAGAATAA+TGG + chr2_3:1553351-1553370 None:intergenic 10.0%
!!! CATCATACTTTTTTTTTTAT+TGG - chr2_3:1553458-1553477 Msa0270280:CDS 15.0%
!! ATATATTCTCAAAACATTTA+AGG - chr2_3:1553604-1553623 Msa0270280:intron 15.0%
!! CATTATACATATGTTAAAAA+AGG + chr2_3:1554090-1554109 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - chr2_3:1554111-1554130 Msa0270280:five_prime_UTR 15.0%
!!! CATCATACTTTTTTTTTTAT+TGG - chr2_3:1553458-1553477 Msa0270280:CDS 15.0%
!! ATATATTCTCAAAACATTTA+AGG - chr2_3:1553604-1553623 Msa0270280:intron 15.0%
!! CATTATACATATGTTAAAAA+AGG + chr2_3:1554090-1554109 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - chr2_3:1554111-1554130 Msa0270280:five_prime_UTR 15.0%
!! TAGAAACATTGAAATCAAAA+TGG + chr2_3:1553905-1553924 None:intergenic 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - chr2_3:1553909-1553928 Msa0270280:CDS 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + chr2_3:1553935-1553954 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + chr2_3:1554258-1554277 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - chr2_3:1554263-1554282 Msa0270280:intron 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + chr2_3:1554304-1554323 None:intergenic 20.0%
!! TAGAAACATTGAAATCAAAA+TGG + chr2_3:1553905-1553924 None:intergenic 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - chr2_3:1553909-1553928 Msa0270280:CDS 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + chr2_3:1553935-1553954 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + chr2_3:1554258-1554277 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - chr2_3:1554263-1554282 Msa0270280:intron 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + chr2_3:1554304-1554323 None:intergenic 20.0%
! AAAAGTGACACTCATATTTA+AGG + chr2_3:1553265-1553284 None:intergenic 25.0%
! AATTAGAACAAAGACAAAAC+AGG - chr2_3:1553698-1553717 Msa0270280:CDS 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - chr2_3:1554341-1554360 Msa0270280:five_prime_UTR 25.0%
! AAAGAGACTAAGAGATTATT+GGG - chr2_3:1554342-1554361 Msa0270280:five_prime_UTR 25.0%
! AAGTCTTAACACTAAAATGA+AGG + chr2_3:1554502-1554521 None:intergenic 25.0%
! AAAAGTGACACTCATATTTA+AGG + chr2_3:1553265-1553284 None:intergenic 25.0%
! AATTAGAACAAAGACAAAAC+AGG - chr2_3:1553698-1553717 Msa0270280:CDS 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - chr2_3:1554341-1554360 Msa0270280:five_prime_UTR 25.0%
! AAAGAGACTAAGAGATTATT+GGG - chr2_3:1554342-1554361 Msa0270280:five_prime_UTR 25.0%
! AAGTCTTAACACTAAAATGA+AGG + chr2_3:1554502-1554521 None:intergenic 25.0%
ACACTATTCATGTGACTTAA+AGG - chr2_3:1553194-1553213 Msa0270280:three_prime_UTR 30.0%
ATTCATGTGACTTAAAGGTT+TGG - chr2_3:1553199-1553218 Msa0270280:three_prime_UTR 30.0%
ATTAAGTTAAGCATCAGTTG+AGG - chr2_3:1553416-1553435 Msa0270280:CDS 30.0%
TTATAAAGCTTAGCTAACTG+TGG - chr2_3:1553481-1553500 Msa0270280:CDS 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - chr2_3:1553518-1553537 Msa0270280:CDS 30.0%
AAGAATCAGACAGAATAACT+AGG + chr2_3:1553652-1553671 None:intergenic 30.0%
TCTATGAGTGCAATATCATT+TGG - chr2_3:1553865-1553884 Msa0270280:CDS 30.0%
ACATGAAACAGGTAGTAAAA+GGG + chr2_3:1554060-1554079 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + chr2_3:1554071-1554090 None:intergenic 30.0%
!! CATCATTTACTCATTGACTT+TGG + chr2_3:1554417-1554436 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - chr2_3:1554418-1554437 Msa0270280:five_prime_UTR 30.0%
ACACTATTCATGTGACTTAA+AGG - chr2_3:1553194-1553213 Msa0270280:three_prime_UTR 30.0%
ATTCATGTGACTTAAAGGTT+TGG - chr2_3:1553199-1553218 Msa0270280:three_prime_UTR 30.0%
ATTAAGTTAAGCATCAGTTG+AGG - chr2_3:1553416-1553435 Msa0270280:CDS 30.0%
TTATAAAGCTTAGCTAACTG+TGG - chr2_3:1553481-1553500 Msa0270280:CDS 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - chr2_3:1553518-1553537 Msa0270280:CDS 30.0%
AAGAATCAGACAGAATAACT+AGG + chr2_3:1553652-1553671 None:intergenic 30.0%
TCTATGAGTGCAATATCATT+TGG - chr2_3:1553865-1553884 Msa0270280:CDS 30.0%
ACATGAAACAGGTAGTAAAA+GGG + chr2_3:1554060-1554079 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + chr2_3:1554071-1554090 None:intergenic 30.0%
!! CATCATTTACTCATTGACTT+TGG + chr2_3:1554417-1554436 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - chr2_3:1554418-1554437 Msa0270280:five_prime_UTR 30.0%
GATTTCATCTCATGTAGTCT+AGG + chr2_3:1553239-1553258 None:intergenic 35.0%
GTGACACTCATATTTAAGGA+TGG + chr2_3:1553261-1553280 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - chr2_3:1553370-1553389 Msa0270280:three_prime_UTR 35.0%
TGTGAGATCAGAATGAAGTT+TGG + chr2_3:1553548-1553567 None:intergenic 35.0%
ACAGTGAAAGATCAAAGATG+TGG - chr2_3:1553749-1553768 Msa0270280:CDS 35.0%
AAAGATGTGGAACGTATACA+CGG - chr2_3:1553762-1553781 Msa0270280:CDS 35.0%
CATTGAAATCAAAATGGCTG+TGG + chr2_3:1553899-1553918 None:intergenic 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + chr2_3:1553982-1554001 None:intergenic 35.0%
ATCTATGCTTCCAACTTATC+AGG - chr2_3:1553984-1554003 Msa0270280:intron 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + chr2_3:1554028-1554047 None:intergenic 35.0%
CACATGAAACAGGTAGTAAA+AGG + chr2_3:1554061-1554080 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - chr2_3:1554144-1554163 Msa0270280:five_prime_UTR 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + chr2_3:1554250-1554269 None:intergenic 35.0%
ATGTAAGCCATGTTTCCATT+TGG - chr2_3:1554384-1554403 Msa0270280:five_prime_UTR 35.0%
GATTTCATCTCATGTAGTCT+AGG + chr2_3:1553239-1553258 None:intergenic 35.0%
GTGACACTCATATTTAAGGA+TGG + chr2_3:1553261-1553280 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - chr2_3:1553370-1553389 Msa0270280:three_prime_UTR 35.0%
TGTGAGATCAGAATGAAGTT+TGG + chr2_3:1553548-1553567 None:intergenic 35.0%
ACAGTGAAAGATCAAAGATG+TGG - chr2_3:1553749-1553768 Msa0270280:CDS 35.0%
AAAGATGTGGAACGTATACA+CGG - chr2_3:1553762-1553781 Msa0270280:CDS 35.0%
CATTGAAATCAAAATGGCTG+TGG + chr2_3:1553899-1553918 None:intergenic 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + chr2_3:1553982-1554001 None:intergenic 35.0%
ATCTATGCTTCCAACTTATC+AGG - chr2_3:1553984-1554003 Msa0270280:intron 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + chr2_3:1554028-1554047 None:intergenic 35.0%
CACATGAAACAGGTAGTAAA+AGG + chr2_3:1554061-1554080 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - chr2_3:1554144-1554163 Msa0270280:five_prime_UTR 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + chr2_3:1554250-1554269 None:intergenic 35.0%
ATGTAAGCCATGTTTCCATT+TGG - chr2_3:1554384-1554403 Msa0270280:five_prime_UTR 35.0%
ACATGAATAGTGTGCCTTGT+GGG + chr2_3:1553187-1553206 None:intergenic 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - chr2_3:1553618-1553637 Msa0270280:intron 40.0%
AAGGTCCATGGAAAAGCTTT+GGG - chr2_3:1553839-1553858 Msa0270280:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + chr2_3:1553958-1553977 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - chr2_3:1554015-1554034 Msa0270280:intron 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + chr2_3:1554024-1554043 None:intergenic 40.0%
AGAACAGCCAAATGGAAACA+TGG + chr2_3:1554394-1554413 None:intergenic 40.0%
ACATGAATAGTGTGCCTTGT+GGG + chr2_3:1553187-1553206 None:intergenic 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - chr2_3:1553618-1553637 Msa0270280:intron 40.0%
AAGGTCCATGGAAAAGCTTT+GGG - chr2_3:1553839-1553858 Msa0270280:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + chr2_3:1553958-1553977 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - chr2_3:1554015-1554034 Msa0270280:intron 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + chr2_3:1554024-1554043 None:intergenic 40.0%
AGAACAGCCAAATGGAAACA+TGG + chr2_3:1554394-1554413 None:intergenic 40.0%
CACATGAATAGTGTGCCTTG+TGG + chr2_3:1553188-1553207 None:intergenic 45.0%
GAAGGTCCATGGAAAAGCTT+TGG - chr2_3:1553838-1553857 Msa0270280:CDS 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + chr2_3:1553847-1553866 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + chr2_3:1553959-1553978 None:intergenic 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + chr2_3:1553997-1554016 None:intergenic 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + chr2_3:1554014-1554033 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - chr2_3:1554156-1554175 Msa0270280:five_prime_UTR 45.0%
TAAGTAGGATATTGCTGCCC+CGG + chr2_3:1554178-1554197 None:intergenic 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + chr2_3:1554223-1554242 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - chr2_3:1554224-1554243 Msa0270280:intron 45.0%
! GACTTTGGAGAACAGCCAAA+TGG + chr2_3:1554402-1554421 None:intergenic 45.0%
CACATGAATAGTGTGCCTTG+TGG + chr2_3:1553188-1553207 None:intergenic 45.0%
GAAGGTCCATGGAAAAGCTT+TGG - chr2_3:1553838-1553857 Msa0270280:CDS 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + chr2_3:1553847-1553866 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + chr2_3:1553959-1553978 None:intergenic 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + chr2_3:1553997-1554016 None:intergenic 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + chr2_3:1554014-1554033 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - chr2_3:1554156-1554175 Msa0270280:five_prime_UTR 45.0%
TAAGTAGGATATTGCTGCCC+CGG + chr2_3:1554178-1554197 None:intergenic 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + chr2_3:1554223-1554242 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - chr2_3:1554224-1554243 Msa0270280:intron 45.0%
! GACTTTGGAGAACAGCCAAA+TGG + chr2_3:1554402-1554421 None:intergenic 45.0%
TTGCTTGCACTTGTCCCACA+AGG - chr2_3:1553170-1553189 Msa0270280:three_prime_UTR 50.0%
TTCTGCCTCTTGAGACAGCA+AGG - chr2_3:1553324-1553343 Msa0270280:three_prime_UTR 50.0%
GTATACACGGCTTCAAACGC+AGG - chr2_3:1553775-1553794 Msa0270280:CDS 50.0%
GGTCCATCACATTCACAGAC+AGG - chr2_3:1553796-1553815 Msa0270280:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + chr2_3:1553802-1553821 None:intergenic 50.0%
CCATCACATTCACAGACAGG+AGG - chr2_3:1553799-1553818 Msa0270280:CDS 50.0%
TCACATTCACAGACAGGAGG+AGG - chr2_3:1553802-1553821 Msa0270280:CDS 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - chr2_3:1553827-1553846 Msa0270280:CDS 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + chr2_3:1553977-1553996 None:intergenic 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - chr2_3:1554157-1554176 Msa0270280:five_prime_UTR 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - chr2_3:1554158-1554177 Msa0270280:five_prime_UTR 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + chr2_3:1554193-1554212 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + chr2_3:1554229-1554248 None:intergenic 50.0%
TTGCTTGCACTTGTCCCACA+AGG - chr2_3:1553170-1553189 Msa0270280:three_prime_UTR 50.0%
TTCTGCCTCTTGAGACAGCA+AGG - chr2_3:1553324-1553343 Msa0270280:three_prime_UTR 50.0%
GTATACACGGCTTCAAACGC+AGG - chr2_3:1553775-1553794 Msa0270280:CDS 50.0%
GGTCCATCACATTCACAGAC+AGG - chr2_3:1553796-1553815 Msa0270280:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + chr2_3:1553802-1553821 None:intergenic 50.0%
CCATCACATTCACAGACAGG+AGG - chr2_3:1553799-1553818 Msa0270280:CDS 50.0%
TCACATTCACAGACAGGAGG+AGG - chr2_3:1553802-1553821 Msa0270280:CDS 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - chr2_3:1553827-1553846 Msa0270280:CDS 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + chr2_3:1553977-1553996 None:intergenic 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - chr2_3:1554157-1554176 Msa0270280:five_prime_UTR 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - chr2_3:1554158-1554177 Msa0270280:five_prime_UTR 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + chr2_3:1554193-1554212 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + chr2_3:1554229-1554248 None:intergenic 50.0%
! ATGGGCCTTGCTGTCTCAAG+AGG + chr2_3:1553332-1553351 None:intergenic 55.0%
CATTCACAGACAGGAGGAGG+AGG - chr2_3:1553805-1553824 Msa0270280:CDS 55.0%
!! AGGAGGAGGAGGAGTTGATG+AGG - chr2_3:1553816-1553835 Msa0270280:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - chr2_3:1553820-1553839 Msa0270280:CDS 55.0%
ACAAGGCAGAGGAGCAGGTT+GGG + chr2_3:1554206-1554225 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - chr2_3:1554210-1554229 Msa0270280:intron 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + chr2_3:1554217-1554236 None:intergenic 55.0%
! ATGGGCCTTGCTGTCTCAAG+AGG + chr2_3:1553332-1553351 None:intergenic 55.0%
CATTCACAGACAGGAGGAGG+AGG - chr2_3:1553805-1553824 Msa0270280:CDS 55.0%
!! AGGAGGAGGAGGAGTTGATG+AGG - chr2_3:1553816-1553835 Msa0270280:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - chr2_3:1553820-1553839 Msa0270280:CDS 55.0%
ACAAGGCAGAGGAGCAGGTT+GGG + chr2_3:1554206-1554225 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - chr2_3:1554210-1554229 Msa0270280:intron 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + chr2_3:1554217-1554236 None:intergenic 55.0%
GACAAGGCAGAGGAGCAGGT+TGG + chr2_3:1554207-1554226 None:intergenic 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + chr2_3:1554211-1554230 None:intergenic 60.0%
CTGCTCCTCTGCCTTGTCCA+AGG - chr2_3:1554209-1554228 Msa0270280:intron 60.0%
GACAAGGCAGAGGAGCAGGT+TGG + chr2_3:1554207-1554226 None:intergenic 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + chr2_3:1554211-1554230 None:intergenic 60.0%
CTGCTCCTCTGCCTTGTCCA+AGG - chr2_3:1554209-1554228 Msa0270280:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 1553150 1554542 1553150 ID=Msa0270280;Name=Msa0270280
chr2_3 mRNA 1553150 1554542 1553150 ID=Msa0270280-mRNA-1;Parent=Msa0270280;Name=Msa0270280-mRNA-1;_AED=0.08;_eAED=0.08;_QI=382|1|1|1|0.33|0.25|4|260|154
chr2_3 exon 1553150 1553559 1553150 ID=Msa0270280-mRNA-1:exon:696;Parent=Msa0270280-mRNA-1
chr2_3 exon 1553642 1553994 1553642 ID=Msa0270280-mRNA-1:exon:695;Parent=Msa0270280-mRNA-1
chr2_3 exon 1554102 1554203 1554102 ID=Msa0270280-mRNA-1:exon:694;Parent=Msa0270280-mRNA-1
chr2_3 exon 1554301 1554542 1554301 ID=Msa0270280-mRNA-1:exon:693;Parent=Msa0270280-mRNA-1
chr2_3 five_prime_UTR 1554301 1554542 1554301 ID=Msa0270280-mRNA-1:five_prime_utr;Parent=Msa0270280-mRNA-1
chr2_3 five_prime_UTR 1554102 1554203 1554102 ID=Msa0270280-mRNA-1:five_prime_utr;Parent=Msa0270280-mRNA-1
chr2_3 five_prime_UTR 1553957 1553994 1553957 ID=Msa0270280-mRNA-1:five_prime_utr;Parent=Msa0270280-mRNA-1
chr2_3 CDS 1553642 1553956 1553642 ID=Msa0270280-mRNA-1:cds;Parent=Msa0270280-mRNA-1
chr2_3 CDS 1553410 1553559 1553410 ID=Msa0270280-mRNA-1:cds;Parent=Msa0270280-mRNA-1
chr2_3 three_prime_UTR 1553150 1553409 1553150 ID=Msa0270280-mRNA-1:three_prime_utr;Parent=Msa0270280-mRNA-1
Gene Sequence

>Msa0270280

ATGAGCGATTTAAACAGTGAAAGATCAAAGATGTGGAACGTATACACGGCTTCAAACGCAGGTCCATCACATTCACAGACAGGAGGAGGAGGAGTTGATGAGGAAGGTCCATGGAAAAGCTTTGGGACTTCTATGAGTGCAATATCATTTGGTTTTGTTGCCACAGCCATTTTGATTTCAATGTTTCTAATAATGGCCATATTTGAACATTTGTTCAAACCCACACAACACTTTTCTTCCACGCCAGAATCTATGCTTCCAACTTATCAGGAACACCACTCACTACCAACCACAAAACAAGGAAATGCACAATCAGTTCTATCATCGTCTTATGCATGTGATTTGTCGGTGTTGATGCCGGGGCAGCAATATCCTACTTACATTGCCCAACCTGCTCCTCTGCCTTGTCCAAGGGAAGAAGCTTATTGGCCTTCTCACCAACAACATTTTGTATTTAACAACTAG
Protein sequence

>Msa0270280

MSDLNSERSKMWNVYTASNAGPSHSQTGGGGVDEEGPWKSFGTSMSAISFGFVATAILISMFLIMAIFEHLFKPTQHFSSTPESMLPTYQEHHSLPTTKQGNAQSVLSSSYACDLSVLMPGQQYPTYIAQPAPLPCPREEAYWPSHQQHFVFNN*