AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0230780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230780 MtrunA17_Chr2g0277071 96.894 161 3 1 1 159 1 161 7.15e-113 317
Msa0230780 MtrunA17_Chr2g0281541 45.714 105 48 2 52 153 53 151 3.95e-24 92.4
Msa0230780 MtrunA17_Chr4g0047901 45.833 120 46 5 43 154 25 133 3.65e-23 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230780 AT1G09812.1 54.464 112 49 2 38 149 16 125 2.12e-29 104
Msa0230780 AT1G58007.4 48.571 105 44 3 47 151 20 114 2.44e-23 89.0
Msa0230780 AT1G58007.3 48.571 105 44 3 47 151 20 114 2.44e-23 89.0
Msa0230780 AT1G58007.2 48.571 105 44 3 47 151 20 114 2.44e-23 89.0
Msa0230780 AT1G11120.2 45.312 128 62 4 32 152 19 145 1.71e-22 88.2
Msa0230780 AT1G11120.1 39.216 153 61 5 32 152 19 171 1.80e-19 80.9
Msa0230780 AT4G28170.1 36.441 118 56 5 23 138 35 135 6.35e-13 62.8
Msa0230780 AT1G58007.1 81.250 32 6 0 47 78 20 51 5.39e-12 58.2

Find 70 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTATTCATGTGACTTAA+AGG 0.213889 2_2:-2999923 Msa0230780:five_prime_UTR
AAAAGTGACACTCATATTTA+AGG 0.221587 2_2:+2999855 None:intergenic
TAAAGAGACTAAGAGATTAT+TGG 0.226828 2_2:-2998762 Msa0230780:three_prime_UTR
GATTTCAATGTTTCTAATAA+TGG 0.253747 2_2:-2999194 Msa0230780:CDS
TTTGAACAAATGTTCAAATA+TGG 0.259962 2_2:+2999171 None:intergenic
GTCCAAGGGAAGAAGCTTAT+TGG 0.267181 2_2:-2998879 Msa0230780:CDS
GTGTGAAGCATTTAAGAAAT+TGG 0.309557 2_2:-2999600 Msa0230780:five_prime_UTR
TAGAAACATTGAAATCAAAA+TGG 0.315037 2_2:+2999201 None:intergenic
AAAGAGACTAAGAGATTATT+GGG 0.315819 2_2:-2998761 Msa0230780:three_prime_UTR
ATCTATGCTTCCAACTTATC+AGG 0.349676 2_2:-2999119 Msa0230780:CDS
TCTATGAGTGCAATATCATT+TGG 0.374376 2_2:-2999238 Msa0230780:CDS
GAAGGTCCATGGAAAAGCTT+TGG 0.389743 2_2:-2999265 Msa0230780:CDS
GGCCAATAAGCTTCTTCCCT+TGG 0.399066 2_2:+2998877 None:intergenic
GTCTTATGCATGTGATTTGT+CGG 0.403359 2_2:-2998959 Msa0230780:CDS
ATTCATGTGACTTAAAGGTT+TGG 0.410583 2_2:-2999918 Msa0230780:five_prime_UTR
ATGTAAGCCATGTTTCCATT+TGG 0.438502 2_2:-2998719 Msa0230780:three_prime_UTR
TGATTTGTCGGTGTTGATGC+CGG 0.444835 2_2:-2998947 Msa0230780:CDS
TGTTAAATACAAAATGTTGT+TGG 0.457080 2_2:+2998848 None:intergenic
CTTGGACAAGGCAGAGGAGC+AGG 0.461138 2_2:+2998895 None:intergenic
ACAAGGCAGAGGAGCAGGTT+GGG 0.462248 2_2:+2998900 None:intergenic
GTAATAGGAATAGGAGTGGT+TGG 0.462562 2_2:-2999976 None:intergenic
GATTTGTCGGTGTTGATGCC+GGG 0.464814 2_2:-2998946 Msa0230780:CDS
AATTAGAACAAAGACAAAAC+AGG 0.469285 2_2:-2999420 Msa0230780:five_prime_UTR
GGTTTCATCTCATGTAGTCT+AGG 0.473423 2_2:+2999881 None:intergenic
TCAAGAAAACGTAATATGTC+AGG 0.477996 2_2:+2998579 None:intergenic
TCTTCCCTTGGACAAGGCAG+AGG 0.486050 2_2:+2998889 None:intergenic
AAGGTCCATGGAAAAGCTTT+GGG 0.488752 2_2:-2999264 Msa0230780:CDS
TAAGTTGGAAGCATAGATTC+TGG 0.501581 2_2:+2999124 None:intergenic
GTGACACTCATATTTAAGGA+TGG 0.502132 2_2:+2999859 None:intergenic
AGGAGGAGGAGGAGTTGATG+AGG 0.503607 2_2:-2999287 Msa0230780:CDS
TCACAGACAGGAGGAGGAGG+AGG 0.507596 2_2:-2999310 Msa0230780:CDS
CTGCTCCTCTGCCTTGTCCA+AGG 0.512022 2_2:-2998894 Msa0230780:CDS
CCTCCTGTCTGTGAATGTGA+TGG 0.513542 2_2:+2999319 None:intergenic
ACAAAATGTTGTTGGTGAGA+AGG 0.517710 2_2:+2998856 None:intergenic
TGTGAGATCAGAATGAAGTT+TGG 0.528238 2_2:+2999573 None:intergenic
TGCTCCTCTGCCTTGTCCAA+GGG 0.530867 2_2:-2998893 Msa0230780:CDS
TGACACTCATATTTAAGGAT+GGG 0.531344 2_2:+2999860 None:intergenic
TAAGTAGGATATTGCTGCCC+CGG 0.537196 2_2:+2998928 None:intergenic
GACAAGGCAGAGGAGCAGGT+TGG 0.540635 2_2:+2998899 None:intergenic
GTATACACGGCTTCAAACGC+AGG 0.541704 2_2:-2999343 Msa0230780:CDS
CATCATTTACTCATTGACTT+CGG 0.541939 2_2:+2998689 None:intergenic
AAGTCTTAACACTAAAATGA+AGG 0.550501 2_2:+2998604 None:intergenic
GAGTTGATGAGGAAGGTCCA+TGG 0.553113 2_2:-2999276 Msa0230780:CDS
CATTGAAATCAAAATGGCTG+TGG 0.558224 2_2:+2999207 None:intergenic
GAGTGGTGTTCCTGATAAGT+TGG 0.558569 2_2:+2999109 None:intergenic
TTCTGCCTCTTGAAACAGCA+AGG 0.561807 2_2:-2999793 Msa0230780:five_prime_UTR
GACTTCGGAGAACAGCCAAA+TGG 0.565451 2_2:+2998704 None:intergenic
GCAGGTTGGGCAATGTAAGT+AGG 0.571068 2_2:+2998913 None:intergenic
ACATGAATAGTGTGCCTTGT+GGG 0.572826 2_2:+2999933 None:intergenic
CATTCACAGACAGGAGGAGG+AGG 0.572987 2_2:-2999313 Msa0230780:CDS
ATGGGCCTTGCTGTTTCAAG+AGG 0.575502 2_2:+2999788 None:intergenic
TAAGTTTGCTTCATCTGACA+AGG 0.587342 2_2:-2999747 Msa0230780:intron
GGTCCATCACATTCACAGAC+AGG 0.587985 2_2:-2999322 Msa0230780:CDS
TCACATTCACAGACAGGAGG+AGG 0.591315 2_2:-2999316 Msa0230780:CDS
GGAGGAGGAGTTGATGAGGA+AGG 0.605051 2_2:-2999283 Msa0230780:CDS
TAAGCTTCTTCCCTTGGACA+AGG 0.606151 2_2:+2998883 None:intergenic
TCACTACCAACCACAAAACA+AGG 0.611869 2_2:-2999088 Msa0230780:CDS
GTGGAAGAAAAGTGTTGTGT+GGG 0.618638 2_2:+2999148 None:intergenic
AGTCAATGAGTAAATGATGA+CGG 0.620366 2_2:-2998685 Msa0230780:three_prime_UTR
TTGCTTGCACTTGTCCCACA+AGG 0.622885 2_2:-2999947 Msa0230780:five_prime_UTR
TTATAAAGCTTAGCTAACTG+TGG 0.627301 2_2:-2999637 Msa0230780:intron
ACAGTGAAAGATCAAAGATG+TGG 0.629582 2_2:-2999369 Msa0230780:CDS
AGAACAGCCAAATGGAAACA+TGG 0.630550 2_2:+2998712 None:intergenic
CGTGGAAGAAAAGTGTTGTG+TGG 0.639502 2_2:+2999147 None:intergenic
ATTTGTCGGTGTTGATGCCG+GGG 0.648111 2_2:-2998945 Msa0230780:CDS
AAAGATGTGGAACGTATACA+CGG 0.657288 2_2:-2999356 Msa0230780:CDS
TAATGTAATAGGAATAGGAG+TGG 0.674683 2_2:-2999980 None:intergenic
CACATGAATAGTGTGCCTTG+TGG 0.676976 2_2:+2999932 None:intergenic
TGGAAGCATAGATTCTGGCG+TGG 0.678051 2_2:+2999129 None:intergenic
CCATCACATTCACAGACAGG+AGG 0.694212 2_2:-2999319 Msa0230780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAAAAAAGAATAAT+GGG + chr2_2:2998783-2998802 None:intergenic 10.0%
!! AAAGAAATAAAAAAGAATAA+TGG + chr2_2:2998784-2998803 None:intergenic 10.0%
!! AAGAAATAAAAAAGAATAAT+GGG + chr2_2:2998783-2998802 None:intergenic 10.0%
!! AAAGAAATAAAAAAGAATAA+TGG + chr2_2:2998784-2998803 None:intergenic 10.0%
!!! TCATCATACTTTTTTTTTAT+TGG - chr2_2:2998890-2998909 Msa0230780:CDS 15.0%
!! ATATATTCTCAAAACATTTA+AGG - chr2_2:2999036-2999055 Msa0230780:intron 15.0%
!! CATTATACATATGTTAAAAA+AGG + chr2_2:2999537-2999556 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - chr2_2:2999558-2999577 Msa0230780:five_prime_UTR 15.0%
!!! TCATCATACTTTTTTTTTAT+TGG - chr2_2:2998890-2998909 Msa0230780:CDS 15.0%
!! ATATATTCTCAAAACATTTA+AGG - chr2_2:2999036-2999055 Msa0230780:intron 15.0%
!! CATTATACATATGTTAAAAA+AGG + chr2_2:2999537-2999556 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - chr2_2:2999558-2999577 Msa0230780:five_prime_UTR 15.0%
!! TAGAAACATTGAAATCAAAA+TGG + chr2_2:2999352-2999371 None:intergenic 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - chr2_2:2999356-2999375 Msa0230780:CDS 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + chr2_2:2999382-2999401 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + chr2_2:2999705-2999724 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - chr2_2:2999710-2999729 Msa0230780:intron 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + chr2_2:2999751-2999770 None:intergenic 20.0%
!! TAGAAACATTGAAATCAAAA+TGG + chr2_2:2999352-2999371 None:intergenic 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - chr2_2:2999356-2999375 Msa0230780:CDS 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + chr2_2:2999382-2999401 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + chr2_2:2999705-2999724 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - chr2_2:2999710-2999729 Msa0230780:intron 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + chr2_2:2999751-2999770 None:intergenic 20.0%
! AAAAGTGACACTCATATTTA+AGG + chr2_2:2998698-2998717 None:intergenic 25.0%
! AATTAGAACAAAGACAAAAC+AGG - chr2_2:2999130-2999149 Msa0230780:CDS 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - chr2_2:2999788-2999807 Msa0230780:five_prime_UTR 25.0%
! AAAGAGACTAAGAGATTATT+GGG - chr2_2:2999789-2999808 Msa0230780:five_prime_UTR 25.0%
! AAGTCTTAACACTAAAATGA+AGG + chr2_2:2999949-2999968 None:intergenic 25.0%
! AAAAGTGACACTCATATTTA+AGG + chr2_2:2998698-2998717 None:intergenic 25.0%
! AATTAGAACAAAGACAAAAC+AGG - chr2_2:2999130-2999149 Msa0230780:CDS 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - chr2_2:2999788-2999807 Msa0230780:five_prime_UTR 25.0%
! AAAGAGACTAAGAGATTATT+GGG - chr2_2:2999789-2999808 Msa0230780:five_prime_UTR 25.0%
! AAGTCTTAACACTAAAATGA+AGG + chr2_2:2999949-2999968 None:intergenic 25.0%
ACACTATTCATGTGACTTAA+AGG - chr2_2:2998627-2998646 Msa0230780:three_prime_UTR 30.0%
ATTCATGTGACTTAAAGGTT+TGG - chr2_2:2998632-2998651 Msa0230780:three_prime_UTR 30.0%
TGACACTCATATTTAAGGAT+GGG + chr2_2:2998693-2998712 None:intergenic 30.0%
ATTAAGTTAAGCATCAGTTG+AGG - chr2_2:2998849-2998868 Msa0230780:CDS 30.0%
TTATAAAGCTTAGCTAACTG+TGG - chr2_2:2998913-2998932 Msa0230780:CDS 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - chr2_2:2998950-2998969 Msa0230780:CDS 30.0%
AAGAATCAGACAGAATAACT+AGG + chr2_2:2999084-2999103 None:intergenic 30.0%
TCTATGAGTGCAATATCATT+TGG - chr2_2:2999312-2999331 Msa0230780:CDS 30.0%
ACATGAAACAGGTAGTAAAA+GGG + chr2_2:2999507-2999526 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + chr2_2:2999518-2999537 None:intergenic 30.0%
!! CATCATTTACTCATTGACTT+CGG + chr2_2:2999864-2999883 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - chr2_2:2999865-2999884 Msa0230780:five_prime_UTR 30.0%
ACACTATTCATGTGACTTAA+AGG - chr2_2:2998627-2998646 Msa0230780:three_prime_UTR 30.0%
ATTCATGTGACTTAAAGGTT+TGG - chr2_2:2998632-2998651 Msa0230780:three_prime_UTR 30.0%
TGACACTCATATTTAAGGAT+GGG + chr2_2:2998693-2998712 None:intergenic 30.0%
ATTAAGTTAAGCATCAGTTG+AGG - chr2_2:2998849-2998868 Msa0230780:CDS 30.0%
TTATAAAGCTTAGCTAACTG+TGG - chr2_2:2998913-2998932 Msa0230780:CDS 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - chr2_2:2998950-2998969 Msa0230780:CDS 30.0%
AAGAATCAGACAGAATAACT+AGG + chr2_2:2999084-2999103 None:intergenic 30.0%
TCTATGAGTGCAATATCATT+TGG - chr2_2:2999312-2999331 Msa0230780:CDS 30.0%
ACATGAAACAGGTAGTAAAA+GGG + chr2_2:2999507-2999526 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + chr2_2:2999518-2999537 None:intergenic 30.0%
!! CATCATTTACTCATTGACTT+CGG + chr2_2:2999864-2999883 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - chr2_2:2999865-2999884 Msa0230780:five_prime_UTR 30.0%
GTGACACTCATATTTAAGGA+TGG + chr2_2:2998694-2998713 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - chr2_2:2998803-2998822 Msa0230780:three_prime_UTR 35.0%
TGTGAGATCAGAATGAAGTT+TGG + chr2_2:2998980-2998999 None:intergenic 35.0%
ACAGTGAAAGATCAAAGATG+TGG - chr2_2:2999181-2999200 Msa0230780:CDS 35.0%
AAAGATGTGGAACGTATACA+CGG - chr2_2:2999194-2999213 Msa0230780:CDS 35.0%
CATTGAAATCAAAATGGCTG+TGG + chr2_2:2999346-2999365 None:intergenic 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + chr2_2:2999429-2999448 None:intergenic 35.0%
ATCTATGCTTCCAACTTATC+AGG - chr2_2:2999431-2999450 Msa0230780:intron 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + chr2_2:2999475-2999494 None:intergenic 35.0%
CACATGAAACAGGTAGTAAA+AGG + chr2_2:2999508-2999527 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - chr2_2:2999591-2999610 Msa0230780:five_prime_UTR 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + chr2_2:2999697-2999716 None:intergenic 35.0%
ATGTAAGCCATGTTTCCATT+TGG - chr2_2:2999831-2999850 Msa0230780:five_prime_UTR 35.0%
GTGACACTCATATTTAAGGA+TGG + chr2_2:2998694-2998713 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - chr2_2:2998803-2998822 Msa0230780:three_prime_UTR 35.0%
TGTGAGATCAGAATGAAGTT+TGG + chr2_2:2998980-2998999 None:intergenic 35.0%
ACAGTGAAAGATCAAAGATG+TGG - chr2_2:2999181-2999200 Msa0230780:CDS 35.0%
AAAGATGTGGAACGTATACA+CGG - chr2_2:2999194-2999213 Msa0230780:CDS 35.0%
CATTGAAATCAAAATGGCTG+TGG + chr2_2:2999346-2999365 None:intergenic 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + chr2_2:2999429-2999448 None:intergenic 35.0%
ATCTATGCTTCCAACTTATC+AGG - chr2_2:2999431-2999450 Msa0230780:intron 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + chr2_2:2999475-2999494 None:intergenic 35.0%
CACATGAAACAGGTAGTAAA+AGG + chr2_2:2999508-2999527 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - chr2_2:2999591-2999610 Msa0230780:five_prime_UTR 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + chr2_2:2999697-2999716 None:intergenic 35.0%
ATGTAAGCCATGTTTCCATT+TGG - chr2_2:2999831-2999850 Msa0230780:five_prime_UTR 35.0%
ACATGAATAGTGTGCCTTGT+GGG + chr2_2:2998620-2998639 None:intergenic 40.0%
GGTTTCATCTCATGTAGTCT+AGG + chr2_2:2998672-2998691 None:intergenic 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - chr2_2:2999050-2999069 Msa0230780:intron 40.0%
AAGGTCCATGGAAAAGCTTT+GGG - chr2_2:2999286-2999305 Msa0230780:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + chr2_2:2999405-2999424 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - chr2_2:2999462-2999481 Msa0230780:intron 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + chr2_2:2999471-2999490 None:intergenic 40.0%
AGAACAGCCAAATGGAAACA+TGG + chr2_2:2999841-2999860 None:intergenic 40.0%
ACATGAATAGTGTGCCTTGT+GGG + chr2_2:2998620-2998639 None:intergenic 40.0%
GGTTTCATCTCATGTAGTCT+AGG + chr2_2:2998672-2998691 None:intergenic 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - chr2_2:2999050-2999069 Msa0230780:intron 40.0%
AAGGTCCATGGAAAAGCTTT+GGG - chr2_2:2999286-2999305 Msa0230780:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + chr2_2:2999405-2999424 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - chr2_2:2999462-2999481 Msa0230780:intron 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + chr2_2:2999471-2999490 None:intergenic 40.0%
AGAACAGCCAAATGGAAACA+TGG + chr2_2:2999841-2999860 None:intergenic 40.0%
CACATGAATAGTGTGCCTTG+TGG + chr2_2:2998621-2998640 None:intergenic 45.0%
TTCTGCCTCTTGAAACAGCA+AGG - chr2_2:2998757-2998776 Msa0230780:three_prime_UTR 45.0%
GAAGGTCCATGGAAAAGCTT+TGG - chr2_2:2999285-2999304 Msa0230780:CDS 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + chr2_2:2999294-2999313 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + chr2_2:2999406-2999425 None:intergenic 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + chr2_2:2999444-2999463 None:intergenic 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + chr2_2:2999461-2999480 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - chr2_2:2999603-2999622 Msa0230780:five_prime_UTR 45.0%
TAAGTAGGATATTGCTGCCC+CGG + chr2_2:2999625-2999644 None:intergenic 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + chr2_2:2999670-2999689 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - chr2_2:2999671-2999690 Msa0230780:intron 45.0%
CACATGAATAGTGTGCCTTG+TGG + chr2_2:2998621-2998640 None:intergenic 45.0%
TTCTGCCTCTTGAAACAGCA+AGG - chr2_2:2998757-2998776 Msa0230780:three_prime_UTR 45.0%
GAAGGTCCATGGAAAAGCTT+TGG - chr2_2:2999285-2999304 Msa0230780:CDS 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + chr2_2:2999294-2999313 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + chr2_2:2999406-2999425 None:intergenic 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + chr2_2:2999444-2999463 None:intergenic 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + chr2_2:2999461-2999480 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - chr2_2:2999603-2999622 Msa0230780:five_prime_UTR 45.0%
TAAGTAGGATATTGCTGCCC+CGG + chr2_2:2999625-2999644 None:intergenic 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + chr2_2:2999670-2999689 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - chr2_2:2999671-2999690 Msa0230780:intron 45.0%
TTGCTTGCACTTGTCCCACA+AGG - chr2_2:2998603-2998622 Msa0230780:three_prime_UTR 50.0%
!! ATGGGCCTTGCTGTTTCAAG+AGG + chr2_2:2998765-2998784 None:intergenic 50.0%
GTATACACGGCTTCAAACGC+AGG - chr2_2:2999207-2999226 Msa0230780:CDS 50.0%
GGTCCATCACATTCACAGAC+AGG - chr2_2:2999228-2999247 Msa0230780:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + chr2_2:2999234-2999253 None:intergenic 50.0%
CCATCACATTCACAGACAGG+AGG - chr2_2:2999231-2999250 Msa0230780:CDS 50.0%
TCACATTCACAGACAGGAGG+AGG - chr2_2:2999234-2999253 Msa0230780:CDS 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - chr2_2:2999274-2999293 Msa0230780:CDS 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + chr2_2:2999424-2999443 None:intergenic 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - chr2_2:2999604-2999623 Msa0230780:five_prime_UTR 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - chr2_2:2999605-2999624 Msa0230780:five_prime_UTR 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + chr2_2:2999640-2999659 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + chr2_2:2999676-2999695 None:intergenic 50.0%
GACTTCGGAGAACAGCCAAA+TGG + chr2_2:2999849-2999868 None:intergenic 50.0%
TTGCTTGCACTTGTCCCACA+AGG - chr2_2:2998603-2998622 Msa0230780:three_prime_UTR 50.0%
!! ATGGGCCTTGCTGTTTCAAG+AGG + chr2_2:2998765-2998784 None:intergenic 50.0%
GTATACACGGCTTCAAACGC+AGG - chr2_2:2999207-2999226 Msa0230780:CDS 50.0%
GGTCCATCACATTCACAGAC+AGG - chr2_2:2999228-2999247 Msa0230780:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + chr2_2:2999234-2999253 None:intergenic 50.0%
CCATCACATTCACAGACAGG+AGG - chr2_2:2999231-2999250 Msa0230780:CDS 50.0%
TCACATTCACAGACAGGAGG+AGG - chr2_2:2999234-2999253 Msa0230780:CDS 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - chr2_2:2999274-2999293 Msa0230780:CDS 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + chr2_2:2999424-2999443 None:intergenic 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - chr2_2:2999604-2999623 Msa0230780:five_prime_UTR 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - chr2_2:2999605-2999624 Msa0230780:five_prime_UTR 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + chr2_2:2999640-2999659 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + chr2_2:2999676-2999695 None:intergenic 50.0%
GACTTCGGAGAACAGCCAAA+TGG + chr2_2:2999849-2999868 None:intergenic 50.0%
CATTCACAGACAGGAGGAGG+AGG - chr2_2:2999237-2999256 Msa0230780:CDS 55.0%
!! AGGAGGAGGAGGAGTTGATG+AGG - chr2_2:2999263-2999282 Msa0230780:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - chr2_2:2999267-2999286 Msa0230780:CDS 55.0%
ACAAGGCAGAGGAGCAGGTT+GGG + chr2_2:2999653-2999672 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - chr2_2:2999657-2999676 Msa0230780:intron 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + chr2_2:2999664-2999683 None:intergenic 55.0%
CATTCACAGACAGGAGGAGG+AGG - chr2_2:2999237-2999256 Msa0230780:CDS 55.0%
!! AGGAGGAGGAGGAGTTGATG+AGG - chr2_2:2999263-2999282 Msa0230780:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - chr2_2:2999267-2999286 Msa0230780:CDS 55.0%
ACAAGGCAGAGGAGCAGGTT+GGG + chr2_2:2999653-2999672 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - chr2_2:2999657-2999676 Msa0230780:intron 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + chr2_2:2999664-2999683 None:intergenic 55.0%
TCACAGACAGGAGGAGGAGG+AGG - chr2_2:2999240-2999259 Msa0230780:CDS 60.0%
GACAAGGCAGAGGAGCAGGT+TGG + chr2_2:2999654-2999673 None:intergenic 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + chr2_2:2999658-2999677 None:intergenic 60.0%
CTGCTCCTCTGCCTTGTCCA+AGG - chr2_2:2999656-2999675 Msa0230780:intron 60.0%
TCACAGACAGGAGGAGGAGG+AGG - chr2_2:2999240-2999259 Msa0230780:CDS 60.0%
GACAAGGCAGAGGAGCAGGT+TGG + chr2_2:2999654-2999673 None:intergenic 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + chr2_2:2999658-2999677 None:intergenic 60.0%
CTGCTCCTCTGCCTTGTCCA+AGG - chr2_2:2999656-2999675 Msa0230780:intron 60.0%
CAGACAGGAGGAGGAGGAGG+AGG - chr2_2:2999243-2999262 Msa0230780:CDS 65.0%
ACAGGAGGAGGAGGAGGAGG+AGG - chr2_2:2999246-2999265 Msa0230780:CDS 65.0%
CAGACAGGAGGAGGAGGAGG+AGG - chr2_2:2999243-2999262 Msa0230780:CDS 65.0%
ACAGGAGGAGGAGGAGGAGG+AGG - chr2_2:2999246-2999265 Msa0230780:CDS 65.0%
GGAGGAGGAGGAGGAGGAGG+AGG - chr2_2:2999249-2999268 Msa0230780:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG - chr2_2:2999249-2999268 Msa0230780:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG - chr2_2:2999249-2999268 Msa0230780:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG - chr2_2:2999249-2999268 Msa0230780:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 2998583 2999989 2998583 ID=Msa0230780;Name=Msa0230780
chr2_2 mRNA 2998583 2999989 2998583 ID=Msa0230780-mRNA-1;Parent=Msa0230780;Name=Msa0230780-mRNA-1;_AED=0.08;_eAED=0.08;_QI=382|1|1|1|0.33|0.25|4|260|159
chr2_2 exon 2998583 2998992 2998583 ID=Msa0230780-mRNA-1:exon:1116;Parent=Msa0230780-mRNA-1
chr2_2 exon 2999075 2999442 2999075 ID=Msa0230780-mRNA-1:exon:1115;Parent=Msa0230780-mRNA-1
chr2_2 exon 2999550 2999651 2999550 ID=Msa0230780-mRNA-1:exon:1114;Parent=Msa0230780-mRNA-1
chr2_2 exon 2999748 2999989 2999748 ID=Msa0230780-mRNA-1:exon:1113;Parent=Msa0230780-mRNA-1
chr2_2 five_prime_UTR 2999748 2999989 2999748 ID=Msa0230780-mRNA-1:five_prime_utr;Parent=Msa0230780-mRNA-1
chr2_2 five_prime_UTR 2999550 2999651 2999550 ID=Msa0230780-mRNA-1:five_prime_utr;Parent=Msa0230780-mRNA-1
chr2_2 five_prime_UTR 2999405 2999442 2999405 ID=Msa0230780-mRNA-1:five_prime_utr;Parent=Msa0230780-mRNA-1
chr2_2 CDS 2999075 2999404 2999075 ID=Msa0230780-mRNA-1:cds;Parent=Msa0230780-mRNA-1
chr2_2 CDS 2998843 2998992 2998843 ID=Msa0230780-mRNA-1:cds;Parent=Msa0230780-mRNA-1
chr2_2 three_prime_UTR 2998583 2998842 2998583 ID=Msa0230780-mRNA-1:three_prime_utr;Parent=Msa0230780-mRNA-1
Gene Sequence

>Msa0230780

ATGAGCGATTTAAACAGTGAAAGATCAAAGATGTGGAACGTATACACGGCTTCAAACGCAGGTCCATCACATTCACAGACAGGAGGAGGAGGAGGAGGAGGAGGAGGAGTTGATGAGGAAGGTCCATGGAAAAGCTTTGGGACTTCTATGAGTGCAATATCATTTGGTTTTGTTGCCACAGCCATTTTGATTTCAATGTTTCTAATAATGGCCATATTTGAACATTTGTTCAAACCCACACAACACTTTTCTTCCACGCCAGAATCTATGCTTCCAACTTATCAGGAACACCACTCACTACCAACCACAAAACAAGGAAATGCACAATCAGTTCTATCATCGTCTTATGCATGTGATTTGTCGGTGTTGATGCCGGGGCAGCAATATCCTACTTACATTGCCCAACCTGCTCCTCTGCCTTGTCCAAGGGAAGAAGCTTATTGGCCTTCTCACCAACAACATTTTGTATTTAACAACTAG
Protein sequence

>Msa0230780

MSDLNSERSKMWNVYTASNAGPSHSQTGGGGGGGGGVDEEGPWKSFGTSMSAISFGFVATAILISMFLIMAIFEHLFKPTQHFSSTPESMLPTYQEHHSLPTTKQGNAQSVLSSSYACDLSVLMPGQQYPTYIAQPAPLPCPREEAYWPSHQQHFVFNN*