AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0230840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230840 MtrunA17_Chr2g0277071 93.789 161 3 1 1 154 1 161 1.05e-108 306
Msa0230840 MtrunA17_Chr2g0281541 45.714 105 48 2 47 148 53 151 4.00e-24 92.0
Msa0230840 MtrunA17_Chr4g0047901 45.833 120 46 5 38 149 25 133 3.47e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230840 AT1G09812.1 46.715 137 59 3 8 144 3 125 1.44e-29 105
Msa0230840 AT1G58007.4 48.571 105 44 3 42 146 20 114 1.64e-23 89.4
Msa0230840 AT1G58007.3 48.571 105 44 3 42 146 20 114 1.64e-23 89.4
Msa0230840 AT1G58007.2 48.571 105 44 3 42 146 20 114 1.64e-23 89.4
Msa0230840 AT1G11120.2 46.341 123 58 4 32 147 24 145 2.07e-22 87.8
Msa0230840 AT1G11120.1 39.597 149 58 5 31 147 23 171 2.27e-19 80.5
Msa0230840 AT4G28170.1 36.207 116 52 6 23 133 37 135 8.94e-12 59.7
Msa0230840 AT1G58007.1 81.250 32 6 0 42 73 20 51 1.78e-11 56.6

Find 72 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATATTCTCAAAACATTTA+AGG 0.096185 2_2:-3047365 Msa0230840:five_prime_UTR
ACACTATTCATGTGACTTAA+AGG 0.213889 2_2:-3047778 Msa0230840:five_prime_UTR
AAAAGTGACACTCATATTTA+AGG 0.221587 2_2:+3047710 None:intergenic
TAAAGAGACTAAGAGATTAT+TGG 0.226828 2_2:-3046623 Msa0230840:three_prime_UTR
GATTTCAATGTTTCTAATAA+TGG 0.253747 2_2:-3047055 Msa0230840:CDS
TTTGAACAAATGTTCAAATA+TGG 0.259962 2_2:+3047032 None:intergenic
GTCCAAGGGAAGAAGCTTAT+TGG 0.267181 2_2:-3046740 Msa0230840:CDS
GTGTGAAGCATTTAAGAAAT+TGG 0.309557 2_2:-3047451 Msa0230840:five_prime_UTR
TAGAAACATTGAAATCAAAA+TGG 0.315037 2_2:+3047062 None:intergenic
AAAGAGACTAAGAGATTATT+GGG 0.315819 2_2:-3046622 Msa0230840:three_prime_UTR
ATCTATGCTTCCAACTTATC+AGG 0.349676 2_2:-3046980 Msa0230840:CDS
TCTATGAGTGCAATATCATT+TGG 0.374376 2_2:-3047099 Msa0230840:CDS
CATTTAAGGCTCGTCTAACT+TGG 0.389061 2_2:-3047351 Msa0230840:five_prime_UTR
GAAGGTCCATGGAAAAGCTT+TGG 0.389743 2_2:-3047126 Msa0230840:CDS
CATCATTTACTCATTGACTT+TGG 0.395198 2_2:+3046550 None:intergenic
GGCCAATAAGCTTCTTCCCT+TGG 0.399066 2_2:+3046738 None:intergenic
GTCTTATGCATGTGATTTGT+CGG 0.403359 2_2:-3046820 Msa0230840:CDS
ATTCATGTGACTTAAAGGTT+TGG 0.410583 2_2:-3047773 Msa0230840:five_prime_UTR
ATGTAAGCCATGTTTCCATT+TGG 0.438502 2_2:-3046580 Msa0230840:three_prime_UTR
TGATTTGTCGGTGTTGATGC+CGG 0.444835 2_2:-3046808 Msa0230840:CDS
TGTTAAATACAAAATGTTGT+TGG 0.457080 2_2:+3046709 None:intergenic
CTTGGACAAGGCAGAGGAGC+AGG 0.461138 2_2:+3046756 None:intergenic
ACAAGGCAGAGGAGCAGGTT+GGG 0.462248 2_2:+3046761 None:intergenic
GTAATAGGAATAGGAGTGGT+TGG 0.462562 2_2:-3047831 None:intergenic
GATTTGTCGGTGTTGATGCC+GGG 0.464814 2_2:-3046807 Msa0230840:CDS
GAACAAAGACAAGACAAAAC+AGG 0.473838 2_2:-3047266 Msa0230840:five_prime_UTR
TCAAGAAAACGTAATATGTC+AGG 0.477996 2_2:+3046440 None:intergenic
TCTTCCCTTGGACAAGGCAG+AGG 0.486050 2_2:+3046750 None:intergenic
AAGGTCCATGGAAAAGCTTT+GGG 0.488752 2_2:-3047125 Msa0230840:CDS
TAAGTTGGAAGCATAGATTC+TGG 0.501581 2_2:+3046985 None:intergenic
AGGAGGAGGAGGAGTTGATG+AGG 0.501669 2_2:-3047148 Msa0230840:CDS
CTGCTCCTCTGCCTTGTCCA+AGG 0.512022 2_2:-3046755 Msa0230840:CDS
CCTCCTGTCTGTGAATGTGA+TGG 0.513542 2_2:+3047165 None:intergenic
GACTTTGGAGAACAGCCAAA+TGG 0.513956 2_2:+3046565 None:intergenic
GATTTCATCTCATGTAGTCT+AGG 0.515564 2_2:+3047736 None:intergenic
ACAAAATGTTGTTGGTGAGA+AGG 0.517710 2_2:+3046717 None:intergenic
TTCTGCCTCTTGAGACAGCA+AGG 0.520372 2_2:-3047648 Msa0230840:five_prime_UTR
TGTGAGATCAGAATGAAGTT+TGG 0.528238 2_2:+3047424 None:intergenic
TGCTCCTCTGCCTTGTCCAA+GGG 0.530867 2_2:-3046754 Msa0230840:CDS
TAAGTAGGATATTGCTGCCC+CGG 0.537196 2_2:+3046789 None:intergenic
GACAAGGCAGAGGAGCAGGT+TGG 0.540635 2_2:+3046760 None:intergenic
GTATACACGGCTTCAAACGC+AGG 0.541704 2_2:-3047189 Msa0230840:CDS
AAGTCTTAACACTAAAATGA+AGG 0.550501 2_2:+3046465 None:intergenic
GAGTTGATGAGGAAGGTCCA+TGG 0.553113 2_2:-3047137 Msa0230840:CDS
GTGACACTCATATTTAAGGA+TGG 0.554314 2_2:+3047714 None:intergenic
CATTGAAATCAAAATGGCTG+TGG 0.558224 2_2:+3047068 None:intergenic
CATTCACAGACAGGAGGAGG+AGG 0.558241 2_2:-3047159 Msa0230840:CDS
GAGTGGTGTTCCTGATAAGT+TGG 0.558569 2_2:+3046970 None:intergenic
GCAGGTTGGGCAATGTAAGT+AGG 0.571068 2_2:+3046774 None:intergenic
ACATGAATAGTGTGCCTTGT+GGG 0.572826 2_2:+3047788 None:intergenic
TAAGTTTGCTTCATCTGACA+AGG 0.587342 2_2:-3047602 Msa0230840:five_prime_UTR
GGTCCATCACATTCACAGAC+AGG 0.587985 2_2:-3047168 Msa0230840:CDS
TCACATTCACAGACAGGAGG+AGG 0.591315 2_2:-3047162 Msa0230840:CDS
ATGGGCCTTGCTGTCTCAAG+AGG 0.592353 2_2:+3047643 None:intergenic
GGAGGAGGAGTTGATGAGGA+AGG 0.605051 2_2:-3047144 Msa0230840:CDS
TAAGCTTCTTCCCTTGGACA+AGG 0.606151 2_2:+3046744 None:intergenic
TCACTACCAACCACAAAACA+AGG 0.611869 2_2:-3046949 Msa0230840:CDS
AGTCAATGAGTAAATGATGA+CGG 0.613545 2_2:-3046546 Msa0230840:three_prime_UTR
AAGAATCAGACAGAATAACT+AGG 0.615224 2_2:+3047320 None:intergenic
GTGGAAGAAAAGTGTTGTGT+GGG 0.618638 2_2:+3047009 None:intergenic
AGAACAGCCAAATGGAAACA+TGG 0.620971 2_2:+3046573 None:intergenic
TTGCTTGCACTTGTCCCACA+AGG 0.622885 2_2:-3047802 Msa0230840:five_prime_UTR
TTATAAAGCTTAGCTAACTG+TGG 0.627301 2_2:-3047488 Msa0230840:five_prime_UTR
ACAGTGAAAGATCAAAGATG+TGG 0.629582 2_2:-3047215 Msa0230840:CDS
AAGTTAAGAAGCATCAGTTG+AGG 0.635181 2_2:-3047553 Msa0230840:five_prime_UTR
CGTGGAAGAAAAGTGTTGTG+TGG 0.639502 2_2:+3047008 None:intergenic
ATTTGTCGGTGTTGATGCCG+GGG 0.648111 2_2:-3046806 Msa0230840:CDS
AAAGATGTGGAACGTATACA+CGG 0.657288 2_2:-3047202 Msa0230840:CDS
TAATGTAATAGGAATAGGAG+TGG 0.674683 2_2:-3047835 None:intergenic
CACATGAATAGTGTGCCTTG+TGG 0.676976 2_2:+3047787 None:intergenic
TGGAAGCATAGATTCTGGCG+TGG 0.678051 2_2:+3046990 None:intergenic
CCATCACATTCACAGACAGG+AGG 0.694212 2_2:-3047165 Msa0230840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAAAAAAGAATAAT+GGG + chr2_2:3046644-3046663 None:intergenic 10.0%
!! AAAGAAATAAAAAAGAATAA+TGG + chr2_2:3046645-3046664 None:intergenic 10.0%
!! AAGAAATAAAAAAGAATAAT+GGG + chr2_2:3046644-3046663 None:intergenic 10.0%
!! AAAGAAATAAAAAAGAATAA+TGG + chr2_2:3046645-3046664 None:intergenic 10.0%
!!! CATCATACTTTTTTTTTTAT+TGG - chr2_2:3046755-3046774 Msa0230840:CDS 15.0%
!! ATATATTCTCAAAACATTTA+AGG - chr2_2:3046901-3046920 Msa0230840:intron 15.0%
!! CATTATACATATGTTAAAAA+AGG + chr2_2:3047392-3047411 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - chr2_2:3047413-3047432 Msa0230840:five_prime_UTR 15.0%
!!! CATCATACTTTTTTTTTTAT+TGG - chr2_2:3046755-3046774 Msa0230840:CDS 15.0%
!! ATATATTCTCAAAACATTTA+AGG - chr2_2:3046901-3046920 Msa0230840:intron 15.0%
!! CATTATACATATGTTAAAAA+AGG + chr2_2:3047392-3047411 None:intergenic 15.0%
!! GTATGTTTATTTATTATTAC+AGG - chr2_2:3047413-3047432 Msa0230840:five_prime_UTR 15.0%
!! TAGAAACATTGAAATCAAAA+TGG + chr2_2:3047207-3047226 None:intergenic 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - chr2_2:3047211-3047230 Msa0230840:CDS 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + chr2_2:3047237-3047256 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + chr2_2:3047560-3047579 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - chr2_2:3047565-3047584 Msa0230840:five_prime_UTR 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + chr2_2:3047606-3047625 None:intergenic 20.0%
!! TAGAAACATTGAAATCAAAA+TGG + chr2_2:3047207-3047226 None:intergenic 20.0%
!!! GATTTCAATGTTTCTAATAA+TGG - chr2_2:3047211-3047230 Msa0230840:CDS 20.0%
!! TTTGAACAAATGTTCAAATA+TGG + chr2_2:3047237-3047256 None:intergenic 20.0%
!! TGTTAAATACAAAATGTTGT+TGG + chr2_2:3047560-3047579 None:intergenic 20.0%
!!! ATTTTGTATTTAACAACTAG+TGG - chr2_2:3047565-3047584 Msa0230840:five_prime_UTR 20.0%
!!! ATCGATTAATAACATTTTGT+AGG + chr2_2:3047606-3047625 None:intergenic 20.0%
! AAAAGTGACACTCATATTTA+AGG + chr2_2:3046559-3046578 None:intergenic 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - chr2_2:3047643-3047662 Msa0230840:five_prime_UTR 25.0%
! AAAGAGACTAAGAGATTATT+GGG - chr2_2:3047644-3047663 Msa0230840:five_prime_UTR 25.0%
! AAGTCTTAACACTAAAATGA+AGG + chr2_2:3047804-3047823 None:intergenic 25.0%
! AAAAGTGACACTCATATTTA+AGG + chr2_2:3046559-3046578 None:intergenic 25.0%
! TAAAGAGACTAAGAGATTAT+TGG - chr2_2:3047643-3047662 Msa0230840:five_prime_UTR 25.0%
! AAAGAGACTAAGAGATTATT+GGG - chr2_2:3047644-3047663 Msa0230840:five_prime_UTR 25.0%
! AAGTCTTAACACTAAAATGA+AGG + chr2_2:3047804-3047823 None:intergenic 25.0%
ACACTATTCATGTGACTTAA+AGG - chr2_2:3046488-3046507 Msa0230840:three_prime_UTR 30.0%
ATTCATGTGACTTAAAGGTT+TGG - chr2_2:3046493-3046512 Msa0230840:three_prime_UTR 30.0%
TTATAAAGCTTAGCTAACTG+TGG - chr2_2:3046778-3046797 Msa0230840:CDS 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - chr2_2:3046815-3046834 Msa0230840:CDS 30.0%
AAGAATCAGACAGAATAACT+AGG + chr2_2:3046949-3046968 None:intergenic 30.0%
TCTATGAGTGCAATATCATT+TGG - chr2_2:3047167-3047186 Msa0230840:CDS 30.0%
ACATGAAACAGGTAGTAAAA+GGG + chr2_2:3047362-3047381 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + chr2_2:3047373-3047392 None:intergenic 30.0%
!! CATCATTTACTCATTGACTT+TGG + chr2_2:3047719-3047738 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - chr2_2:3047720-3047739 Msa0230840:five_prime_UTR 30.0%
ACACTATTCATGTGACTTAA+AGG - chr2_2:3046488-3046507 Msa0230840:three_prime_UTR 30.0%
ATTCATGTGACTTAAAGGTT+TGG - chr2_2:3046493-3046512 Msa0230840:three_prime_UTR 30.0%
TTATAAAGCTTAGCTAACTG+TGG - chr2_2:3046778-3046797 Msa0230840:CDS 30.0%
! GTGTGAAGCATTTAAGAAAT+TGG - chr2_2:3046815-3046834 Msa0230840:CDS 30.0%
AAGAATCAGACAGAATAACT+AGG + chr2_2:3046949-3046968 None:intergenic 30.0%
TCTATGAGTGCAATATCATT+TGG - chr2_2:3047167-3047186 Msa0230840:CDS 30.0%
ACATGAAACAGGTAGTAAAA+GGG + chr2_2:3047362-3047381 None:intergenic 30.0%
AAGGAAAAAACACATGAAAC+AGG + chr2_2:3047373-3047392 None:intergenic 30.0%
!! CATCATTTACTCATTGACTT+TGG + chr2_2:3047719-3047738 None:intergenic 30.0%
AGTCAATGAGTAAATGATGA+CGG - chr2_2:3047720-3047739 Msa0230840:five_prime_UTR 30.0%
GATTTCATCTCATGTAGTCT+AGG + chr2_2:3046533-3046552 None:intergenic 35.0%
GTGACACTCATATTTAAGGA+TGG + chr2_2:3046555-3046574 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - chr2_2:3046664-3046683 Msa0230840:three_prime_UTR 35.0%
!! AAGTTAAGAAGCATCAGTTG+AGG - chr2_2:3046713-3046732 Msa0230840:CDS 35.0%
TGTGAGATCAGAATGAAGTT+TGG + chr2_2:3046845-3046864 None:intergenic 35.0%
GAACAAAGACAAGACAAAAC+AGG - chr2_2:3047000-3047019 Msa0230840:CDS 35.0%
ACAGTGAAAGATCAAAGATG+TGG - chr2_2:3047051-3047070 Msa0230840:CDS 35.0%
AAAGATGTGGAACGTATACA+CGG - chr2_2:3047064-3047083 Msa0230840:CDS 35.0%
CATTGAAATCAAAATGGCTG+TGG + chr2_2:3047201-3047220 None:intergenic 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + chr2_2:3047284-3047303 None:intergenic 35.0%
ATCTATGCTTCCAACTTATC+AGG - chr2_2:3047286-3047305 Msa0230840:five_prime_UTR 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + chr2_2:3047330-3047349 None:intergenic 35.0%
CACATGAAACAGGTAGTAAA+AGG + chr2_2:3047363-3047382 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - chr2_2:3047446-3047465 Msa0230840:five_prime_UTR 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + chr2_2:3047552-3047571 None:intergenic 35.0%
ATGTAAGCCATGTTTCCATT+TGG - chr2_2:3047686-3047705 Msa0230840:five_prime_UTR 35.0%
GATTTCATCTCATGTAGTCT+AGG + chr2_2:3046533-3046552 None:intergenic 35.0%
GTGACACTCATATTTAAGGA+TGG + chr2_2:3046555-3046574 None:intergenic 35.0%
! TAAGTTTGCTTCATCTGACA+AGG - chr2_2:3046664-3046683 Msa0230840:three_prime_UTR 35.0%
!! AAGTTAAGAAGCATCAGTTG+AGG - chr2_2:3046713-3046732 Msa0230840:CDS 35.0%
TGTGAGATCAGAATGAAGTT+TGG + chr2_2:3046845-3046864 None:intergenic 35.0%
GAACAAAGACAAGACAAAAC+AGG - chr2_2:3047000-3047019 Msa0230840:CDS 35.0%
ACAGTGAAAGATCAAAGATG+TGG - chr2_2:3047051-3047070 Msa0230840:CDS 35.0%
AAAGATGTGGAACGTATACA+CGG - chr2_2:3047064-3047083 Msa0230840:CDS 35.0%
CATTGAAATCAAAATGGCTG+TGG + chr2_2:3047201-3047220 None:intergenic 35.0%
! TAAGTTGGAAGCATAGATTC+TGG + chr2_2:3047284-3047303 None:intergenic 35.0%
ATCTATGCTTCCAACTTATC+AGG - chr2_2:3047286-3047305 Msa0230840:five_prime_UTR 35.0%
!!! TTGTGCATTTCCTTGTTTTG+TGG + chr2_2:3047330-3047349 None:intergenic 35.0%
CACATGAAACAGGTAGTAAA+AGG + chr2_2:3047363-3047382 None:intergenic 35.0%
! GTCTTATGCATGTGATTTGT+CGG - chr2_2:3047446-3047465 Msa0230840:five_prime_UTR 35.0%
! ACAAAATGTTGTTGGTGAGA+AGG + chr2_2:3047552-3047571 None:intergenic 35.0%
ATGTAAGCCATGTTTCCATT+TGG - chr2_2:3047686-3047705 Msa0230840:five_prime_UTR 35.0%
ACATGAATAGTGTGCCTTGT+GGG + chr2_2:3046481-3046500 None:intergenic 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - chr2_2:3046915-3046934 Msa0230840:intron 40.0%
AAGGTCCATGGAAAAGCTTT+GGG - chr2_2:3047141-3047160 Msa0230840:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + chr2_2:3047260-3047279 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - chr2_2:3047317-3047336 Msa0230840:five_prime_UTR 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + chr2_2:3047326-3047345 None:intergenic 40.0%
AGAACAGCCAAATGGAAACA+TGG + chr2_2:3047696-3047715 None:intergenic 40.0%
ACATGAATAGTGTGCCTTGT+GGG + chr2_2:3046481-3046500 None:intergenic 40.0%
! CATTTAAGGCTCGTCTAACT+TGG - chr2_2:3046915-3046934 Msa0230840:intron 40.0%
AAGGTCCATGGAAAAGCTTT+GGG - chr2_2:3047141-3047160 Msa0230840:CDS 40.0%
!! GTGGAAGAAAAGTGTTGTGT+GGG + chr2_2:3047260-3047279 None:intergenic 40.0%
TCACTACCAACCACAAAACA+AGG - chr2_2:3047317-3047336 Msa0230840:five_prime_UTR 40.0%
!!! GCATTTCCTTGTTTTGTGGT+TGG + chr2_2:3047326-3047345 None:intergenic 40.0%
AGAACAGCCAAATGGAAACA+TGG + chr2_2:3047696-3047715 None:intergenic 40.0%
CACATGAATAGTGTGCCTTG+TGG + chr2_2:3046482-3046501 None:intergenic 45.0%
GAAGGTCCATGGAAAAGCTT+TGG - chr2_2:3047140-3047159 Msa0230840:CDS 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + chr2_2:3047149-3047168 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + chr2_2:3047261-3047280 None:intergenic 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + chr2_2:3047299-3047318 None:intergenic 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + chr2_2:3047316-3047335 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - chr2_2:3047458-3047477 Msa0230840:five_prime_UTR 45.0%
TAAGTAGGATATTGCTGCCC+CGG + chr2_2:3047480-3047499 None:intergenic 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + chr2_2:3047525-3047544 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - chr2_2:3047526-3047545 Msa0230840:five_prime_UTR 45.0%
! GACTTTGGAGAACAGCCAAA+TGG + chr2_2:3047704-3047723 None:intergenic 45.0%
CACATGAATAGTGTGCCTTG+TGG + chr2_2:3046482-3046501 None:intergenic 45.0%
GAAGGTCCATGGAAAAGCTT+TGG - chr2_2:3047140-3047159 Msa0230840:CDS 45.0%
! GAAGTCCCAAAGCTTTTCCA+TGG + chr2_2:3047149-3047168 None:intergenic 45.0%
!! CGTGGAAGAAAAGTGTTGTG+TGG + chr2_2:3047261-3047280 None:intergenic 45.0%
GAGTGGTGTTCCTGATAAGT+TGG + chr2_2:3047299-3047318 None:intergenic 45.0%
!!! GTTTTGTGGTTGGTAGTGAG+TGG + chr2_2:3047316-3047335 None:intergenic 45.0%
!! TGATTTGTCGGTGTTGATGC+CGG - chr2_2:3047458-3047477 Msa0230840:five_prime_UTR 45.0%
TAAGTAGGATATTGCTGCCC+CGG + chr2_2:3047480-3047499 None:intergenic 45.0%
TAAGCTTCTTCCCTTGGACA+AGG + chr2_2:3047525-3047544 None:intergenic 45.0%
GTCCAAGGGAAGAAGCTTAT+TGG - chr2_2:3047526-3047545 Msa0230840:five_prime_UTR 45.0%
! GACTTTGGAGAACAGCCAAA+TGG + chr2_2:3047704-3047723 None:intergenic 45.0%
TTGCTTGCACTTGTCCCACA+AGG - chr2_2:3046464-3046483 Msa0230840:three_prime_UTR 50.0%
TTCTGCCTCTTGAGACAGCA+AGG - chr2_2:3046618-3046637 Msa0230840:three_prime_UTR 50.0%
GTATACACGGCTTCAAACGC+AGG - chr2_2:3047077-3047096 Msa0230840:CDS 50.0%
GGTCCATCACATTCACAGAC+AGG - chr2_2:3047098-3047117 Msa0230840:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + chr2_2:3047104-3047123 None:intergenic 50.0%
CCATCACATTCACAGACAGG+AGG - chr2_2:3047101-3047120 Msa0230840:CDS 50.0%
TCACATTCACAGACAGGAGG+AGG - chr2_2:3047104-3047123 Msa0230840:CDS 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - chr2_2:3047129-3047148 Msa0230840:CDS 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + chr2_2:3047279-3047298 None:intergenic 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - chr2_2:3047459-3047478 Msa0230840:five_prime_UTR 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - chr2_2:3047460-3047479 Msa0230840:five_prime_UTR 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + chr2_2:3047495-3047514 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + chr2_2:3047531-3047550 None:intergenic 50.0%
TTGCTTGCACTTGTCCCACA+AGG - chr2_2:3046464-3046483 Msa0230840:three_prime_UTR 50.0%
TTCTGCCTCTTGAGACAGCA+AGG - chr2_2:3046618-3046637 Msa0230840:three_prime_UTR 50.0%
GTATACACGGCTTCAAACGC+AGG - chr2_2:3047077-3047096 Msa0230840:CDS 50.0%
GGTCCATCACATTCACAGAC+AGG - chr2_2:3047098-3047117 Msa0230840:CDS 50.0%
CCTCCTGTCTGTGAATGTGA+TGG + chr2_2:3047104-3047123 None:intergenic 50.0%
CCATCACATTCACAGACAGG+AGG - chr2_2:3047101-3047120 Msa0230840:CDS 50.0%
TCACATTCACAGACAGGAGG+AGG - chr2_2:3047104-3047123 Msa0230840:CDS 50.0%
!! GAGTTGATGAGGAAGGTCCA+TGG - chr2_2:3047129-3047148 Msa0230840:CDS 50.0%
! TGGAAGCATAGATTCTGGCG+TGG + chr2_2:3047279-3047298 None:intergenic 50.0%
!! GATTTGTCGGTGTTGATGCC+GGG - chr2_2:3047459-3047478 Msa0230840:five_prime_UTR 50.0%
!! ATTTGTCGGTGTTGATGCCG+GGG - chr2_2:3047460-3047479 Msa0230840:five_prime_UTR 50.0%
! GCAGGTTGGGCAATGTAAGT+AGG + chr2_2:3047495-3047514 None:intergenic 50.0%
GGCCAATAAGCTTCTTCCCT+TGG + chr2_2:3047531-3047550 None:intergenic 50.0%
! ATGGGCCTTGCTGTCTCAAG+AGG + chr2_2:3046626-3046645 None:intergenic 55.0%
CATTCACAGACAGGAGGAGG+AGG - chr2_2:3047107-3047126 Msa0230840:CDS 55.0%
!! AGGAGGAGGAGGAGTTGATG+AGG - chr2_2:3047118-3047137 Msa0230840:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - chr2_2:3047122-3047141 Msa0230840:CDS 55.0%
ACAAGGCAGAGGAGCAGGTT+GGG + chr2_2:3047508-3047527 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - chr2_2:3047512-3047531 Msa0230840:five_prime_UTR 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + chr2_2:3047519-3047538 None:intergenic 55.0%
! ATGGGCCTTGCTGTCTCAAG+AGG + chr2_2:3046626-3046645 None:intergenic 55.0%
CATTCACAGACAGGAGGAGG+AGG - chr2_2:3047107-3047126 Msa0230840:CDS 55.0%
!! AGGAGGAGGAGGAGTTGATG+AGG - chr2_2:3047118-3047137 Msa0230840:CDS 55.0%
!! GGAGGAGGAGTTGATGAGGA+AGG - chr2_2:3047122-3047141 Msa0230840:CDS 55.0%
ACAAGGCAGAGGAGCAGGTT+GGG + chr2_2:3047508-3047527 None:intergenic 55.0%
TGCTCCTCTGCCTTGTCCAA+GGG - chr2_2:3047512-3047531 Msa0230840:five_prime_UTR 55.0%
TCTTCCCTTGGACAAGGCAG+AGG + chr2_2:3047519-3047538 None:intergenic 55.0%
GACAAGGCAGAGGAGCAGGT+TGG + chr2_2:3047509-3047528 None:intergenic 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + chr2_2:3047513-3047532 None:intergenic 60.0%
CTGCTCCTCTGCCTTGTCCA+AGG - chr2_2:3047511-3047530 Msa0230840:five_prime_UTR 60.0%
GACAAGGCAGAGGAGCAGGT+TGG + chr2_2:3047509-3047528 None:intergenic 60.0%
CTTGGACAAGGCAGAGGAGC+AGG + chr2_2:3047513-3047532 None:intergenic 60.0%
CTGCTCCTCTGCCTTGTCCA+AGG - chr2_2:3047511-3047530 Msa0230840:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 3046444 3047844 3046444 ID=Msa0230840;Name=Msa0230840
chr2_2 mRNA 3046444 3047844 3046444 ID=Msa0230840-mRNA-1;Parent=Msa0230840;Name=Msa0230840-mRNA-1;_AED=0.00;_eAED=0.00;_QI=594|1|1|1|1|1|2|260|154
chr2_2 exon 3046444 3046853 3046444 ID=Msa0230840-mRNA-1:exon:1112;Parent=Msa0230840-mRNA-1
chr2_2 exon 3046936 3047844 3046936 ID=Msa0230840-mRNA-1:exon:1111;Parent=Msa0230840-mRNA-1
chr2_2 five_prime_UTR 3047251 3047844 3047251 ID=Msa0230840-mRNA-1:five_prime_utr;Parent=Msa0230840-mRNA-1
chr2_2 CDS 3046936 3047250 3046936 ID=Msa0230840-mRNA-1:cds;Parent=Msa0230840-mRNA-1
chr2_2 CDS 3046704 3046853 3046704 ID=Msa0230840-mRNA-1:cds;Parent=Msa0230840-mRNA-1
chr2_2 three_prime_UTR 3046444 3046703 3046444 ID=Msa0230840-mRNA-1:three_prime_utr;Parent=Msa0230840-mRNA-1
Gene Sequence

>Msa0230840

ATGAGCGATTTAAACAGTGAAAGATCAAAGATGTGGAACGTATACACGGCTTCAAACGCAGGTCCATCACATTCACAGACAGGAGGAGGAGGAGTTGATGAGGAAGGTCCATGGAAAAGCTTTGGGACTTCTATGAGTGCAATATCATTTGGTTTTGTTGCCACAGCCATTTTGATTTCAATGTTTCTAATAATGGCCATATTTGAACATTTGTTCAAACCCACACAACACTTTTCTTCCACGCCAGAATCTATGCTTCCAACTTATCAGGAACACCACTCACTACCAACCACAAAACAAGGAAATGCACAATCAGTTCTATCATCGTCTTATGCATGTGATTTGTCGGTGTTGATGCCGGGGCAGCAATATCCTACTTACATTGCCCAACCTGCTCCTCTGCCTTGTCCAAGGGAAGAAGCTTATTGGCCTTCTCACCAACAACATTTTGTATTTAACAACTAG
Protein sequence

>Msa0230840

MSDLNSERSKMWNVYTASNAGPSHSQTGGGGVDEEGPWKSFGTSMSAISFGFVATAILISMFLIMAIFEHLFKPTQHFSSTPESMLPTYQEHHSLPTTKQGNAQSVLSSSYACDLSVLMPGQQYPTYIAQPAPLPCPREEAYWPSHQQHFVFNN*