AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0304550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0304550 MtrunA17_Chr2g0332971 91.693 313 9 1 1 296 92 404 0.0 578
Msa0304550 MtrunA17_Chr5g0418411 44.625 307 154 5 1 294 93 396 6.22e-81 250
Msa0304550 MtrunA17_Chr8g0337571 44.408 304 150 5 1 294 94 388 2.47e-79 245
Msa0304550 MtrunA17_Chr3g0100091 43.464 306 154 4 1 294 48 346 3.83e-79 244
Msa0304550 MtrunA17_Chr3g0121891 44.839 310 148 5 1 294 93 395 4.97e-79 245
Msa0304550 MtrunA17_Chr4g0043661 42.258 310 161 5 1 294 87 394 4.35e-78 242
Msa0304550 MtrunA17_Chr3g0121881 41.967 305 164 4 1 294 97 399 2.28e-77 241
Msa0304550 MtrunA17_Chr4g0018151 46.358 302 151 5 1 294 142 440 4.12e-73 231
Msa0304550 MtrunA17_Chr2g0332801 40.268 298 161 2 3 284 123 419 2.55e-71 227
Msa0304550 MtrunA17_Chr6g0461181 37.464 347 158 5 1 294 94 434 8.99e-71 225
Msa0304550 MtrunA17_Chr8g0336481 44.788 259 125 5 1 248 16 267 1.59e-68 214
Msa0304550 MtrunA17_Chr8g0337581 41.639 305 157 7 1 294 95 389 5.16e-68 216
Msa0304550 MtrunA17_Chr7g0228411 40.541 333 151 8 1 293 104 429 9.63e-67 214
Msa0304550 MtrunA17_Chr8g0365901 38.255 298 166 2 1 282 126 421 2.45e-66 214
Msa0304550 MtrunA17_Chr8g0337511 44.068 236 125 3 48 283 3 231 9.04e-64 201
Msa0304550 MtrunA17_Chr8g0337601 44.444 243 129 2 52 294 4 240 2.25e-63 200
Msa0304550 MtrunA17_Chr1g0173451 39.852 271 153 2 1 262 137 406 1.23e-62 204
Msa0304550 MtrunA17_Chr6g0449961 37.987 308 174 7 1 296 107 409 8.25e-61 199
Msa0304550 MtrunA17_Chr1g0150291 36.577 298 170 4 1 285 109 400 6.58e-60 196
Msa0304550 MtrunA17_Chr4g0020771 38.078 281 154 2 1 262 133 412 6.10e-59 194
Msa0304550 MtrunA17_Chr1g0150301 34.899 298 175 4 1 285 91 382 1.66e-57 189
Msa0304550 MtrunA17_Chr1g0194231 34.688 320 183 5 1 295 144 462 4.19e-55 185
Msa0304550 MtrunA17_Chr5g0413141 33.007 306 181 4 1 284 120 423 2.98e-54 182
Msa0304550 MtrunA17_Chr7g0230891 33.987 306 183 6 3 294 100 400 3.13e-54 181
Msa0304550 MtrunA17_Chr4g0052621 32.915 319 188 5 1 294 126 443 4.89e-54 182
Msa0304550 MtrunA17_Chr3g0089021 40.357 280 150 2 1 264 131 409 9.49e-54 181
Msa0304550 MtrunA17_Chr8g0365891 32.153 339 182 7 1 294 169 504 2.40e-53 181
Msa0304550 MtrunA17_Chr5g0413411 31.953 338 183 7 1 294 183 517 4.18e-53 181
Msa0304550 MtrunA17_Chr7g0270361 32.500 320 191 5 1 296 130 448 1.34e-52 178
Msa0304550 MtrunA17_Chr2g0291841 35.144 313 176 9 1 294 58 362 1.76e-51 173
Msa0304550 MtrunA17_Chr4g0043431 39.453 256 139 4 22 262 116 370 1.09e-50 172
Msa0304550 MtrunA17_Chr2g0303941 37.458 299 162 9 1 282 89 379 1.57e-50 171
Msa0304550 MtrunA17_Chr1g0195221 37.276 279 153 6 12 273 110 383 2.75e-50 171
Msa0304550 MtrunA17_Chr2g0307811 36.014 286 160 5 1 265 84 367 4.43e-50 170
Msa0304550 MtrunA17_Chr2g0307831 35.315 286 162 5 1 265 84 367 2.10e-48 166
Msa0304550 MtrunA17_Chr2g0307821 35.315 286 162 5 1 265 84 367 2.10e-48 166
Msa0304550 MtrunA17_Chr3g0093011 35.417 240 152 1 45 281 9 248 5.04e-48 161
Msa0304550 MtrunA17_Chr7g0257701 40.435 230 126 3 1 220 162 390 6.63e-48 164
Msa0304550 MtrunA17_Chr4g0067631 36.042 283 157 5 3 265 117 395 3.49e-46 160
Msa0304550 MtrunA17_Chr2g0293681 31.132 318 161 7 1 294 128 411 2.09e-44 156
Msa0304550 MtrunA17_Chr8g0348981 31.615 291 181 3 22 294 108 398 2.19e-44 155
Msa0304550 MtrunA17_Chr8g0337351 42.690 171 97 1 60 229 1 171 8.70e-40 137
Msa0304550 MtrunA17_Chr8g0337551 48.485 132 68 0 48 179 3 134 1.97e-38 132
Msa0304550 MtrunA17_Chr1g0149241 30.968 310 159 6 1 294 92 362 4.22e-38 138
Msa0304550 MtrunA17_Chr5g0425811 34.454 238 129 5 1 217 41 272 7.59e-38 135
Msa0304550 MtrunA17_Chr7g0257721 39.487 195 101 2 1 179 137 330 5.42e-37 134
Msa0304550 MtrunA17_Chr5g0447901 35.808 229 128 6 1 211 84 311 2.95e-36 132
Msa0304550 MtrunA17_Chr8g0337521 40.741 189 93 4 1 179 94 273 4.52e-35 128
Msa0304550 MtrunA17_Chr2g0295451 36.564 227 122 5 3 210 118 341 4.76e-32 122
Msa0304550 MtrunA17_Chr7g0273191 44.348 115 64 0 49 163 24 138 4.47e-29 108
Msa0304550 MtrunA17_Chr8g0337561 42.609 115 60 2 182 296 8 116 3.14e-22 89.4
Msa0304550 MtrunA17_Chr8g0337531 39.231 130 72 3 165 294 9 131 3.20e-21 87.4
Msa0304550 MtrunA17_Chr7g0270821 26.582 316 175 11 24 288 143 452 1.25e-20 91.7
Msa0304550 MtrunA17_Chr7g0255311 31.100 209 113 7 16 197 154 358 8.90e-19 86.3
Msa0304550 MtrunA17_Chr2g0301091 27.152 302 182 10 13 284 144 437 6.02e-18 83.6
Msa0304550 MtrunA17_Chr4g0070281 26.159 302 185 9 13 284 163 456 1.02e-17 83.2
Msa0304550 MtrunA17_Chr3g0130251 29.808 208 107 6 28 204 179 378 2.93e-17 81.6
Msa0304550 MtrunA17_Chr1g0202641 30.412 194 105 6 23 190 154 343 3.70e-17 82.0
Msa0304550 MtrunA17_Chr1g0202641 26.403 303 172 11 23 289 654 941 1.26e-16 80.1
Msa0304550 MtrunA17_Chr8g0387391 25.651 269 151 8 23 246 143 407 1.14e-16 80.1
Msa0304550 MtrunA17_Chr1g0202651 29.897 194 106 6 23 190 154 343 1.38e-16 79.7
Msa0304550 MtrunA17_Chr8g0373171 40.230 87 52 0 48 134 5 91 2.30e-15 71.2
Msa0304550 MtrunA17_Chr1g0202621 24.444 270 157 11 23 251 147 410 7.38e-15 74.3
Msa0304550 MtrunA17_Chr1g0193961 27.103 214 123 4 24 210 143 350 7.94e-15 74.3
Msa0304550 MtrunA17_Chr3g0095221 30.693 202 103 7 26 195 143 339 2.16e-14 73.2
Msa0304550 MtrunA17_Chr5g0442221 28.972 214 115 7 25 206 140 348 7.47e-14 71.6
Msa0304550 MtrunA17_Chr8g0348491 24.232 293 191 6 23 289 154 441 3.51e-13 69.7
Msa0304550 MtrunA17_Chr7g0248451 29.897 194 106 6 23 190 154 343 3.98e-13 69.3
Msa0304550 MtrunA17_Chr7g0273181 35.714 84 53 1 202 285 6 88 5.87e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0304550 AT5G17200.1 45.342 322 144 5 1 294 102 419 1.25e-84 260
Msa0304550 AT5G27530.1 44.781 297 157 2 1 294 117 409 1.95e-80 251
Msa0304550 AT5G44830.1 42.517 294 167 2 1 294 39 330 7.55e-78 240
Msa0304550 AT4G32380.1 42.123 292 158 2 1 281 91 382 2.75e-77 243
Msa0304550 AT4G35670.1 43.322 307 155 4 1 294 88 388 8.02e-73 229
Msa0304550 AT5G44840.1 39.145 304 173 4 1 294 88 389 1.53e-70 223
Msa0304550 AT4G35670.2 43.706 286 142 4 22 294 2 281 3.78e-70 219
Msa0304550 AT1G80170.1 36.877 301 174 1 1 285 115 415 2.60e-67 216
Msa0304550 AT3G15720.4 39.274 303 171 5 3 294 25 325 1.28e-66 213
Msa0304550 AT4G32375.1 40.647 278 155 2 13 280 4 281 2.83e-66 213
Msa0304550 AT3G15720.2 40.000 300 170 5 3 294 88 385 2.90e-66 214
Msa0304550 AT3G15720.1 39.274 303 171 5 3 294 89 389 6.98e-66 213
Msa0304550 AT3G15720.3 39.274 303 171 5 3 294 88 388 7.50e-66 213
Msa0304550 AT1G70500.1 37.919 298 167 2 1 282 128 423 1.02e-64 210
Msa0304550 AT1G56710.1 38.050 318 173 3 1 295 112 428 1.96e-64 209
Msa0304550 AT1G23460.2 37.919 298 167 2 1 282 104 399 1.06e-62 204
Msa0304550 AT1G23460.1 37.299 311 176 3 1 294 128 436 1.79e-62 204
Msa0304550 AT4G32380.2 36.897 290 135 2 1 281 91 341 1.44e-61 201
Msa0304550 AT3G57510.1 39.583 288 153 4 3 272 132 416 2.46e-61 201
Msa0304550 AT5G14650.1 37.540 309 175 7 1 294 113 418 3.90e-60 197
Msa0304550 AT2G41850.1 40.569 281 146 4 3 265 132 409 1.07e-57 191
Msa0304550 AT1G80140.1 36.156 307 181 5 1 294 32 336 2.16e-56 185
Msa0304550 AT4G01890.1 35.127 316 177 6 6 294 134 448 2.31e-56 189
Msa0304550 AT1G48100.1 33.750 320 186 6 1 295 141 459 2.91e-56 189
Msa0304550 AT1G10640.1 33.136 338 179 6 1 294 181 515 8.35e-56 189
Msa0304550 AT3G07970.1 37.770 278 155 2 1 262 133 408 2.41e-55 185
Msa0304550 AT2G43880.1 37.540 309 168 7 3 294 94 394 1.87e-54 182
Msa0304550 AT1G02460.1 35.404 322 180 8 1 295 153 473 2.44e-54 184
Msa0304550 AT4G32370.1 41.410 227 120 3 1 214 100 326 4.51e-54 179
Msa0304550 AT1G60590.1 33.724 341 176 8 1 294 185 522 4.70e-54 184
Msa0304550 AT1G05650.1 36.789 299 167 8 1 283 89 381 1.14e-53 180
Msa0304550 AT1G05660.1 35.161 310 179 8 1 294 89 392 3.17e-53 179
Msa0304550 AT1G65570.1 37.374 297 167 7 1 283 95 386 5.04e-53 178
Msa0304550 AT3G26610.1 34.740 308 174 6 1 283 128 433 5.77e-53 180
Msa0304550 AT2G43860.1 36.334 311 177 7 1 296 96 400 1.28e-51 175
Msa0304550 AT5G48140.1 36.742 264 144 4 22 265 98 358 4.12e-50 171
Msa0304550 AT2G43890.1 37.079 267 149 6 3 254 92 354 5.18e-50 170
Msa0304550 AT4G18180.1 36.882 263 143 3 22 264 122 381 9.00e-50 170
Msa0304550 AT3G07820.1 35.606 264 147 4 22 265 98 358 6.02e-48 165
Msa0304550 AT3G59850.1 37.050 278 154 8 1 262 86 358 1.51e-47 164
Msa0304550 AT2G26620.1 32.459 305 179 5 1 284 93 391 1.72e-47 164
Msa0304550 AT2G40310.1 32.075 318 185 7 1 294 93 403 2.39e-47 163
Msa0304550 AT1G17150.1 32.692 312 188 7 1 294 94 401 2.55e-47 163
Msa0304550 AT3G59850.2 37.050 278 154 8 1 262 130 402 3.10e-47 164
Msa0304550 AT1G78400.1 35.036 274 158 5 1 257 96 366 1.14e-46 162
Msa0304550 AT1G02790.1 36.719 256 141 5 15 254 122 372 1.18e-46 162
Msa0304550 AT3G07830.1 33.221 298 168 5 22 291 99 393 1.31e-46 161
Msa0304550 AT1G43080.1 32.390 318 184 7 1 294 93 403 1.48e-46 161
Msa0304550 AT2G15460.1 33.443 305 178 8 1 284 93 393 2.43e-46 160
Msa0304550 AT4G13760.1 33.929 280 155 6 1 257 93 365 5.09e-46 160
Msa0304550 AT2G43870.1 34.727 311 176 12 1 294 84 384 7.04e-46 159
Msa0304550 AT2G15450.1 33.115 305 179 8 1 284 93 393 9.37e-46 159
Msa0304550 AT1G43090.1 32.680 306 175 7 1 282 93 391 1.22e-45 160
Msa0304550 AT2G15470.1 32.698 315 187 8 1 294 93 403 1.34e-45 159
Msa0304550 AT1G43100.1 32.680 306 175 7 1 282 93 391 1.51e-45 159
Msa0304550 AT3G07840.1 37.109 256 138 4 22 257 99 351 3.61e-45 157
Msa0304550 AT3G07850.1 35.691 311 168 8 1 291 129 427 4.91e-45 158
Msa0304550 AT2G33160.1 33.775 302 176 8 1 282 94 391 5.62e-44 159
Msa0304550 AT3G14040.1 35.048 311 170 8 1 291 130 428 1.34e-43 154
Msa0304550 AT1G80170.2 39.086 197 104 1 1 181 115 311 2.46e-41 146
Msa0304550 AT2G43880.2 38.865 229 123 5 3 217 94 319 3.28e-40 143
Msa0304550 AT5G39910.1 36.486 222 135 3 1 220 100 317 1.49e-38 139
Msa0304550 AT5G14650.2 40.206 194 102 3 1 181 113 305 1.53e-36 133
Msa0304550 AT3G62110.1 25.862 290 183 6 31 293 149 433 1.47e-20 91.7
Msa0304550 AT3G62110.2 25.862 290 183 6 31 293 149 433 1.47e-20 91.7
Msa0304550 AT4G33440.1 32.701 211 104 6 24 204 172 374 8.00e-20 89.7
Msa0304550 AT3G42950.1 27.425 299 183 8 13 283 154 446 4.85e-18 84.3
Msa0304550 AT2G23900.1 26.144 306 180 12 24 295 156 449 1.22e-17 83.2
Msa0304550 AT3G48950.1 27.700 213 118 7 18 198 139 347 2.09e-16 79.7
Msa0304550 AT5G41870.1 30.909 220 122 6 13 206 134 349 4.37e-16 78.6
Msa0304550 AT4G23820.1 30.693 202 105 7 25 195 136 333 9.05e-16 77.4
Msa0304550 AT3G61490.4 31.053 190 100 6 28 190 261 446 1.78e-15 77.0
Msa0304550 AT3G61490.5 31.053 190 100 6 28 190 163 348 2.87e-15 76.3
Msa0304550 AT3G61490.2 31.053 190 100 6 28 190 148 333 3.27e-15 75.9
Msa0304550 AT3G61490.3 31.053 190 100 6 28 190 148 333 3.27e-15 75.9
Msa0304550 AT3G61490.1 31.053 190 100 6 28 190 148 333 3.27e-15 75.9
Msa0304550 AT1G19170.1 25.828 302 186 10 13 284 176 469 1.83e-14 73.9
Msa0304550 AT4G23500.1 24.549 277 160 10 16 247 160 432 1.83e-12 67.8

Find 71 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAATCCATGTGTTTATT+TGG 0.080796 2_3:+61873783 None:intergenic
ATATCAATGCCATCAGTATT+AGG 0.225865 2_3:+61872955 None:intergenic
TGCTATCGATTTCAAATAAA+AGG 0.252766 2_3:-61873075 Msa0304550:CDS
TTTGATTAACACAATAAAAT+TGG 0.261059 2_3:+61872200 None:intergenic
AACTCTAACAAGCTGCAAAA+AGG 0.263487 2_3:-61873019 Msa0304550:CDS
ACCAATCCATGTGTTTATTT+GGG 0.310797 2_3:+61873784 None:intergenic
TAAAATTGGTCAATCATAAT+GGG 0.312360 2_3:+61872214 None:intergenic
GGTTAGAGCACCCCACATTT+TGG 0.323169 2_3:+61871960 None:intergenic
GGTCAATCATAATGGGGTTA+TGG 0.323679 2_3:+61872221 None:intergenic
TGCAGCTTGTTAGAGTTATA+TGG 0.325682 2_3:+61873025 None:intergenic
ATAAAATTGGTCAATCATAA+TGG 0.333850 2_3:+61872213 None:intergenic
AAGGAGAAAACCTTCATCTT+AGG 0.335531 2_3:+61871902 None:intergenic
TTCAACTAGCATATATTATA+TGG 0.344021 2_3:+61871797 None:intergenic
AAAACAAAATCGGAATATTC+AGG 0.348846 2_3:-61873809 Msa0304550:CDS
TGTTGTAGCATTGGATCATT+GGG 0.360291 2_3:-61872587 Msa0304550:intron
ACGTTGACTGAAACATTAAC+TGG 0.363004 2_3:-61872515 Msa0304550:CDS
CTTAACTCTTTCATTGCAAC+TGG 0.372034 2_3:-61872907 Msa0304550:intron
TTGAGCAAGCAGTTTAGAAT+TGG 0.374243 2_3:+61871842 None:intergenic
TTGTTGTAGCATTGGATCAT+TGG 0.378542 2_3:-61872588 Msa0304550:intron
GTCAATCATAATGGGGTTAT+GGG 0.383263 2_3:+61872222 None:intergenic
AGGCTCTTCCATGAGCATTA+TGG 0.387155 2_3:+61871876 None:intergenic
TATCAATGCCATCAGTATTA+GGG 0.410347 2_3:+61872956 None:intergenic
CAAGCAGTTTAGAATTGGTT+TGG 0.412319 2_3:+61871847 None:intergenic
AACATCCGTTTAATTGCTCC+TGG 0.419774 2_3:-61872985 Msa0304550:CDS
AGAGTTATATGGCTTCTTGC+TGG 0.423641 2_3:+61873036 None:intergenic
GGTTTGATTATAAGTGGAAA+AGG 0.427621 2_3:-61873746 Msa0304550:CDS
GTGGTCCAGGACATGGTATC+AGG 0.443137 2_3:-61872717 Msa0304550:intron
AGTGGAAAAGGGACTATTGA+TGG 0.444615 2_3:-61873734 Msa0304550:CDS
ATGCACATCCTCTACAATAT+CGG 0.446275 2_3:+61872550 None:intergenic
GTCAGTGATGTAACATATAG+AGG 0.449992 2_3:-61872031 Msa0304550:CDS
TGTTGCACCTAAAACAAAAT+CGG 0.452223 2_3:-61873819 Msa0304550:CDS
GTTTGATTATAAGTGGAAAA+GGG 0.469200 2_3:-61873745 Msa0304550:CDS
TCCTTCTGCCATAATGCTCA+TGG 0.470413 2_3:-61871884 Msa0304550:CDS
GTGAATGGTTTGATTATAAG+TGG 0.472070 2_3:-61873752 Msa0304550:CDS
GATTGCATTGCTATTAGTGC+TGG 0.472202 2_3:-61872772 Msa0304550:CDS
ATTGGTTTCTCATTTGTGAA+TGG 0.475752 2_3:-61873767 Msa0304550:CDS
ATCTGCTGTTCCTAAGATGA+AGG 0.477417 2_3:-61871912 Msa0304550:CDS
ATTTCTGAACAGGGTGGAGG+TGG 0.479630 2_3:-61872289 Msa0304550:intron
TCCCAAATAAACACATGGAT+TGG 0.484532 2_3:-61873785 Msa0304550:CDS
TCTCTCCAGGAGCAATTAAA+CGG 0.494330 2_3:+61872980 None:intergenic
GTAACATATAGAGGGATTAG+TGG 0.503191 2_3:-61872022 Msa0304550:CDS
TGTATTTCTGAACAGGGTGG+AGG 0.533598 2_3:-61872292 Msa0304550:intron
ACTAACCTGATACCATGTCC+TGG 0.541363 2_3:+61872712 None:intergenic
TTTGAACTGCGACCAAAATG+TGG 0.542733 2_3:-61871972 Msa0304550:CDS
AGTAAGAATCAAGACAAAGC+AGG 0.556906 2_3:-61872492 Msa0304550:intron
TCAGTATTAGGGCTCTCTCC+AGG 0.559038 2_3:+61872967 None:intergenic
TGCAACTATTATGATTCCAA+AGG 0.560466 2_3:+61871757 None:intergenic
CATAAACACGATCAAATACA+AGG 0.567940 2_3:+61871939 None:intergenic
ACTGCTATAACATGTGGTCC+AGG 0.572929 2_3:-61872730 Msa0304550:CDS
TAGAATTGGTTTGGTGTGTG+AGG 0.574986 2_3:+61871856 None:intergenic
AGGGTGGAGGTGGATTTGCT+AGG 0.585416 2_3:-61872279 Msa0304550:CDS
GTGGAGGTGGATTTGCTAGG+AGG 0.587173 2_3:-61872276 Msa0304550:CDS
TTGTAGCATTGGATCATTGG+GGG 0.588643 2_3:-61872585 Msa0304550:intron
AAAGGGACTATTGATGGTCG+AGG 0.592324 2_3:-61873728 Msa0304550:CDS
GGAGAGAGCCCTAATACTGA+TGG 0.597997 2_3:-61872964 Msa0304550:CDS
TCCATGAGCATTATGGCAGA+AGG 0.604867 2_3:+61871883 None:intergenic
ATAACATGTGGTCCAGGACA+TGG 0.613113 2_3:-61872724 Msa0304550:CDS
CAGGATCCCAAATAAACACA+TGG 0.616392 2_3:-61873790 Msa0304550:CDS
TCAGTGATGTAACATATAGA+GGG 0.625634 2_3:-61872030 Msa0304550:CDS
AAAATAACTGCTATAACATG+TGG 0.629212 2_3:-61872736 Msa0304550:CDS
TTGAACTGCGACCAAAATGT+GGG 0.634447 2_3:-61871971 Msa0304550:CDS
AAGCACTTGAATGTCTCTTG+AGG 0.634460 2_3:+61872927 None:intergenic
GCTCAAGTAGCTAGCTACCT+CGG 0.636157 2_3:-61871825 Msa0304550:exon
AAAATTGGTCAATCATAATG+GGG 0.641528 2_3:+61872215 None:intergenic
GTTGTAGCATTGGATCATTG+GGG 0.669066 2_3:-61872586 Msa0304550:intron
TGGAGACACCGATATTGTAG+AGG 0.670467 2_3:-61872558 Msa0304550:CDS
TGGAACATCATTAACAGACA+AGG 0.670604 2_3:-61872002 Msa0304550:CDS
TTGATGGTCGAGGCTCTATG+TGG 0.678132 2_3:-61873718 Msa0304550:intron
AAAGACACGGTAATCGTACA+CGG 0.682275 2_3:+61871694 None:intergenic
TATATTATATGGTAGCTCCG+AGG 0.755322 2_3:+61871808 None:intergenic
TGAACTGCGACCAAAATGTG+GGG 0.756518 2_3:-61871970 Msa0304550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATATAAAAATTTACTTA+CGG + chr2_3:61871874-61871893 None:intergenic 10.0%
!!! TTATATGTATAGTTTTTTTA+AGG - chr2_3:61871885-61871904 Msa0304550:CDS 10.0%
!!! AATAAGTATTTTCTTAATTA+AGG + chr2_3:61872922-61872941 None:intergenic 10.0%
!!! AGAAATTTAGTTTATTTTTT+GGG + chr2_3:61873425-61873444 None:intergenic 10.0%
!!! TAGAAATTTAGTTTATTTTT+TGG + chr2_3:61873426-61873445 None:intergenic 10.0%
!! TACATATAAAAATTTACTTA+CGG + chr2_3:61871874-61871893 None:intergenic 10.0%
!!! TTATATGTATAGTTTTTTTA+AGG - chr2_3:61871885-61871904 Msa0304550:CDS 10.0%
!!! AATAAGTATTTTCTTAATTA+AGG + chr2_3:61872922-61872941 None:intergenic 10.0%
!!! AGAAATTTAGTTTATTTTTT+GGG + chr2_3:61873425-61873444 None:intergenic 10.0%
!!! TAGAAATTTAGTTTATTTTT+TGG + chr2_3:61873426-61873445 None:intergenic 10.0%
!! ACATATAAAAATTTACTTAC+GGG + chr2_3:61871873-61871892 None:intergenic 15.0%
!! TGAAATGTTAAGTTAAAAAA+AGG + chr2_3:61872180-61872199 None:intergenic 15.0%
!!! ATTTTTTTTTTTCCTTTTTC+AGG - chr2_3:61872262-61872281 Msa0304550:CDS 15.0%
!!! TTTTTTTTTTTCCTTTTTCA+GGG - chr2_3:61872263-61872282 Msa0304550:CDS 15.0%
!! ATTGTATCTAATCATATTAT+AGG - chr2_3:61872736-61872755 Msa0304550:CDS 15.0%
!! AATAAAGAAATTAATACTCA+TGG + chr2_3:61873114-61873133 None:intergenic 15.0%
!!! TTTCACTTATACTATAATTT+TGG - chr2_3:61873160-61873179 Msa0304550:intron 15.0%
!! TTTGATTAACACAATAAAAT+TGG + chr2_3:61873338-61873357 None:intergenic 15.0%
!!! ATTTTATTGTGTTAATCAAA+TGG - chr2_3:61873338-61873357 Msa0304550:intron 15.0%
!! AGTAACATATATAAGAAAAA+TGG + chr2_3:61873401-61873420 None:intergenic 15.0%
!!! GAAATTTAGTTTATTTTTTG+GGG + chr2_3:61873424-61873443 None:intergenic 15.0%
!! ACATATAAAAATTTACTTAC+GGG + chr2_3:61871873-61871892 None:intergenic 15.0%
!! TGAAATGTTAAGTTAAAAAA+AGG + chr2_3:61872180-61872199 None:intergenic 15.0%
!!! ATTTTTTTTTTTCCTTTTTC+AGG - chr2_3:61872262-61872281 Msa0304550:CDS 15.0%
!!! TTTTTTTTTTTCCTTTTTCA+GGG - chr2_3:61872263-61872282 Msa0304550:CDS 15.0%
!! ATTGTATCTAATCATATTAT+AGG - chr2_3:61872736-61872755 Msa0304550:CDS 15.0%
!! AATAAAGAAATTAATACTCA+TGG + chr2_3:61873114-61873133 None:intergenic 15.0%
!!! TTTCACTTATACTATAATTT+TGG - chr2_3:61873160-61873179 Msa0304550:intron 15.0%
!! TTTGATTAACACAATAAAAT+TGG + chr2_3:61873338-61873357 None:intergenic 15.0%
!!! ATTTTATTGTGTTAATCAAA+TGG - chr2_3:61873338-61873357 Msa0304550:intron 15.0%
!! AGTAACATATATAAGAAAAA+TGG + chr2_3:61873401-61873420 None:intergenic 15.0%
!!! GAAATTTAGTTTATTTTTTG+GGG + chr2_3:61873424-61873443 None:intergenic 15.0%
!!! TTTTTTTTTTCCTTTTTCAG+GGG - chr2_3:61872264-61872283 Msa0304550:CDS 20.0%
!! AAAATAAATACCAGTTCATA+TGG + chr2_3:61872401-61872420 None:intergenic 20.0%
!!! TATTTATTTTTGTGTGTGTA+TGG - chr2_3:61872411-61872430 Msa0304550:intron 20.0%
!!! ATTTTAATTTGTTGTAGCAT+TGG - chr2_3:61872939-61872958 Msa0304550:CDS 20.0%
!! ACCAAAATAACTAACATTAT+TGG - chr2_3:61873079-61873098 Msa0304550:CDS 20.0%
!!! ACCAATAATGTTAGTTATTT+TGG + chr2_3:61873083-61873102 None:intergenic 20.0%
!! ATAAGTGAAAAACCTTATAT+AGG + chr2_3:61873150-61873169 None:intergenic 20.0%
!! TAAAATTGGTCAATCATAAT+GGG + chr2_3:61873324-61873343 None:intergenic 20.0%
!! ATAAAATTGGTCAATCATAA+TGG + chr2_3:61873325-61873344 None:intergenic 20.0%
!! TTCAACTAGCATATATTATA+TGG + chr2_3:61873741-61873760 None:intergenic 20.0%
!!! TTTTTTTTTTCCTTTTTCAG+GGG - chr2_3:61872264-61872283 Msa0304550:CDS 20.0%
!! AAAATAAATACCAGTTCATA+TGG + chr2_3:61872401-61872420 None:intergenic 20.0%
!!! TATTTATTTTTGTGTGTGTA+TGG - chr2_3:61872411-61872430 Msa0304550:intron 20.0%
!!! ATTTTAATTTGTTGTAGCAT+TGG - chr2_3:61872939-61872958 Msa0304550:CDS 20.0%
!! ACCAAAATAACTAACATTAT+TGG - chr2_3:61873079-61873098 Msa0304550:CDS 20.0%
!!! ACCAATAATGTTAGTTATTT+TGG + chr2_3:61873083-61873102 None:intergenic 20.0%
!! ATAAGTGAAAAACCTTATAT+AGG + chr2_3:61873150-61873169 None:intergenic 20.0%
!! TAAAATTGGTCAATCATAAT+GGG + chr2_3:61873324-61873343 None:intergenic 20.0%
!! ATAAAATTGGTCAATCATAA+TGG + chr2_3:61873325-61873344 None:intergenic 20.0%
!! TTCAACTAGCATATATTATA+TGG + chr2_3:61873741-61873760 None:intergenic 20.0%
!!! GAATATTCCGATTTTGTTTT+AGG + chr2_3:61871726-61871745 None:intergenic 25.0%
! AAAACAAAATCGGAATATTC+AGG - chr2_3:61871726-61871745 Msa0304550:three_prime_UTR 25.0%
! GTTTGATTATAAGTGGAAAA+GGG - chr2_3:61871790-61871809 Msa0304550:three_prime_UTR 25.0%
! TATAAAAATTTACTTACGGG+TGG + chr2_3:61871870-61871889 None:intergenic 25.0%
!! ATAGGTCATTTAAGTTTCAA+AGG - chr2_3:61871951-61871970 Msa0304550:CDS 25.0%
!! ATATGTCCTTTAAGTTTCTA+AGG - chr2_3:61871980-61871999 Msa0304550:CDS 25.0%
!!! AAATTTCTAAGGTTTCAGAT+AGG + chr2_3:61872074-61872093 None:intergenic 25.0%
! CTGAAACCTTAGAAATTTAA+AGG - chr2_3:61872076-61872095 Msa0304550:intron 25.0%
! ATAGGTCCTTTAAATTTCTA+AGG + chr2_3:61872085-61872104 None:intergenic 25.0%
! AAGTTTCAAATGTTACAGAT+AGG + chr2_3:61872103-61872122 None:intergenic 25.0%
!! CTGTAACATTTGAAACTTAA+AGG - chr2_3:61872105-61872124 Msa0304550:intron 25.0%
! TGATGCAAGTATTACTATAA+AGG + chr2_3:61872233-61872252 None:intergenic 25.0%
!! TCTATAGTATGTATTTTGCA+AGG + chr2_3:61872338-61872357 None:intergenic 25.0%
!!! TGCTATCGATTTCAAATAAA+AGG - chr2_3:61872460-61872479 Msa0304550:intron 25.0%
! AAAATAACTGCTATAACATG+TGG - chr2_3:61872799-61872818 Msa0304550:intron 25.0%
!! TTTCTATTGTATTTCTGAAC+AGG - chr2_3:61873236-61873255 Msa0304550:intron 25.0%
!! TTCTATTGTATTTCTGAACA+GGG - chr2_3:61873237-61873256 Msa0304550:intron 25.0%
! AAAATTGGTCAATCATAATG+GGG + chr2_3:61873323-61873342 None:intergenic 25.0%
!!! ATCTTTTTTTCTTCTTCCTT+TGG - chr2_3:61873762-61873781 Msa0304550:CDS 25.0%
!!! GAATATTCCGATTTTGTTTT+AGG + chr2_3:61871726-61871745 None:intergenic 25.0%
! AAAACAAAATCGGAATATTC+AGG - chr2_3:61871726-61871745 Msa0304550:three_prime_UTR 25.0%
! GTTTGATTATAAGTGGAAAA+GGG - chr2_3:61871790-61871809 Msa0304550:three_prime_UTR 25.0%
! TATAAAAATTTACTTACGGG+TGG + chr2_3:61871870-61871889 None:intergenic 25.0%
!! ATAGGTCATTTAAGTTTCAA+AGG - chr2_3:61871951-61871970 Msa0304550:CDS 25.0%
!! ATATGTCCTTTAAGTTTCTA+AGG - chr2_3:61871980-61871999 Msa0304550:CDS 25.0%
!!! AAATTTCTAAGGTTTCAGAT+AGG + chr2_3:61872074-61872093 None:intergenic 25.0%
! CTGAAACCTTAGAAATTTAA+AGG - chr2_3:61872076-61872095 Msa0304550:intron 25.0%
! ATAGGTCCTTTAAATTTCTA+AGG + chr2_3:61872085-61872104 None:intergenic 25.0%
! AAGTTTCAAATGTTACAGAT+AGG + chr2_3:61872103-61872122 None:intergenic 25.0%
!! CTGTAACATTTGAAACTTAA+AGG - chr2_3:61872105-61872124 Msa0304550:intron 25.0%
! TGATGCAAGTATTACTATAA+AGG + chr2_3:61872233-61872252 None:intergenic 25.0%
!! TCTATAGTATGTATTTTGCA+AGG + chr2_3:61872338-61872357 None:intergenic 25.0%
!!! TGCTATCGATTTCAAATAAA+AGG - chr2_3:61872460-61872479 Msa0304550:intron 25.0%
! AAAATAACTGCTATAACATG+TGG - chr2_3:61872799-61872818 Msa0304550:intron 25.0%
!! TTTCTATTGTATTTCTGAAC+AGG - chr2_3:61873236-61873255 Msa0304550:intron 25.0%
!! TTCTATTGTATTTCTGAACA+GGG - chr2_3:61873237-61873256 Msa0304550:intron 25.0%
! AAAATTGGTCAATCATAATG+GGG + chr2_3:61873323-61873342 None:intergenic 25.0%
!!! ATCTTTTTTTCTTCTTCCTT+TGG - chr2_3:61873762-61873781 Msa0304550:CDS 25.0%
TGTTGCACCTAAAACAAAAT+CGG - chr2_3:61871716-61871735 Msa0304550:three_prime_UTR 30.0%
ACCAATCCATGTGTTTATTT+GGG + chr2_3:61871754-61871773 None:intergenic 30.0%
AACCAATCCATGTGTTTATT+TGG + chr2_3:61871755-61871774 None:intergenic 30.0%
! ATTGGTTTCTCATTTGTGAA+TGG - chr2_3:61871768-61871787 Msa0304550:three_prime_UTR 30.0%
GTGAATGGTTTGATTATAAG+TGG - chr2_3:61871783-61871802 Msa0304550:three_prime_UTR 30.0%
GGTTTGATTATAAGTGGAAA+AGG - chr2_3:61871789-61871808 Msa0304550:three_prime_UTR 30.0%
GTTACAAACGTGAAACTTAA+AGG + chr2_3:61872136-61872155 None:intergenic 30.0%
CTGAAACCTTAGAAACTTAA+AGG + chr2_3:61871989-61872008 None:intergenic 30.0%
!! AAGTTTCTAAGGTTTCAGAT+AGG - chr2_3:61871991-61872010 Msa0304550:CDS 30.0%
GAAACAAACTCGAAACTTAA+AGG + chr2_3:61872018-61872037 None:intergenic 30.0%
GTTACAAACGTGAAACTTAA+AGG - chr2_3:61872136-61872155 Msa0304550:intron 30.0%
TATCAATGCCATCAGTATTA+GGG + chr2_3:61872582-61872601 None:intergenic 30.0%
ATATCAATGCCATCAGTATT+AGG + chr2_3:61872583-61872602 None:intergenic 30.0%
!! TAATTTTGGTCACATGACTT+AGG - chr2_3:61873174-61873193 Msa0304550:intron 30.0%
TCAGTGATGTAACATATAGA+GGG - chr2_3:61873505-61873524 Msa0304550:intron 30.0%
CATAAACACGATCAAATACA+AGG + chr2_3:61873599-61873618 None:intergenic 30.0%
TGCAACTATTATGATTCCAA+AGG + chr2_3:61873781-61873800 None:intergenic 30.0%
TGTTGCACCTAAAACAAAAT+CGG - chr2_3:61871716-61871735 Msa0304550:three_prime_UTR 30.0%
ACCAATCCATGTGTTTATTT+GGG + chr2_3:61871754-61871773 None:intergenic 30.0%
AACCAATCCATGTGTTTATT+TGG + chr2_3:61871755-61871774 None:intergenic 30.0%
! ATTGGTTTCTCATTTGTGAA+TGG - chr2_3:61871768-61871787 Msa0304550:three_prime_UTR 30.0%
GTGAATGGTTTGATTATAAG+TGG - chr2_3:61871783-61871802 Msa0304550:three_prime_UTR 30.0%
GGTTTGATTATAAGTGGAAA+AGG - chr2_3:61871789-61871808 Msa0304550:three_prime_UTR 30.0%
GTTACAAACGTGAAACTTAA+AGG + chr2_3:61872136-61872155 None:intergenic 30.0%
CTGAAACCTTAGAAACTTAA+AGG + chr2_3:61871989-61872008 None:intergenic 30.0%
!! AAGTTTCTAAGGTTTCAGAT+AGG - chr2_3:61871991-61872010 Msa0304550:CDS 30.0%
GAAACAAACTCGAAACTTAA+AGG + chr2_3:61872018-61872037 None:intergenic 30.0%
GTTACAAACGTGAAACTTAA+AGG - chr2_3:61872136-61872155 Msa0304550:intron 30.0%
TATCAATGCCATCAGTATTA+GGG + chr2_3:61872582-61872601 None:intergenic 30.0%
ATATCAATGCCATCAGTATT+AGG + chr2_3:61872583-61872602 None:intergenic 30.0%
!! TAATTTTGGTCACATGACTT+AGG - chr2_3:61873174-61873193 Msa0304550:intron 30.0%
TCAGTGATGTAACATATAGA+GGG - chr2_3:61873505-61873524 Msa0304550:intron 30.0%
CATAAACACGATCAAATACA+AGG + chr2_3:61873599-61873618 None:intergenic 30.0%
TGCAACTATTATGATTCCAA+AGG + chr2_3:61873781-61873800 None:intergenic 30.0%
TCCCAAATAAACACATGGAT+TGG - chr2_3:61871750-61871769 Msa0304550:three_prime_UTR 35.0%
AAAAATTTACTTACGGGTGG+TGG + chr2_3:61871867-61871886 None:intergenic 35.0%
GTTTCACGTTTGTAACAGAT+AGG - chr2_3:61871933-61871952 Msa0304550:CDS 35.0%
!! GTTTCGAGTTTGTTTCAGAT+AGG - chr2_3:61872022-61872041 Msa0304550:CDS 35.0%
TTGTGTGTGTATGGATGTAT+AGG - chr2_3:61872420-61872439 Msa0304550:intron 35.0%
!! TGCAGCTTGTTAGAGTTATA+TGG + chr2_3:61872513-61872532 None:intergenic 35.0%
AACTCTAACAAGCTGCAAAA+AGG - chr2_3:61872516-61872535 Msa0304550:CDS 35.0%
ACTCTAACAAGCTGCAAAAA+GGG - chr2_3:61872517-61872536 Msa0304550:CDS 35.0%
CTTAACTCTTTCATTGCAAC+TGG - chr2_3:61872628-61872647 Msa0304550:intron 35.0%
! TTGTTGTAGCATTGGATCAT+TGG - chr2_3:61872947-61872966 Msa0304550:CDS 35.0%
! TGTTGTAGCATTGGATCATT+GGG - chr2_3:61872948-61872967 Msa0304550:CDS 35.0%
ATGCACATCCTCTACAATAT+CGG + chr2_3:61872988-61873007 None:intergenic 35.0%
! ACGTTGACTGAAACATTAAC+TGG - chr2_3:61873020-61873039 Msa0304550:CDS 35.0%
AGTAAGAATCAAGACAAAGC+AGG - chr2_3:61873043-61873062 Msa0304550:CDS 35.0%
CGTACTACTACTCCTATATA+AGG - chr2_3:61873135-61873154 Msa0304550:intron 35.0%
! TATTGTATTTCTGAACAGGG+TGG - chr2_3:61873240-61873259 Msa0304550:intron 35.0%
! GTCAATCATAATGGGGTTAT+GGG + chr2_3:61873316-61873335 None:intergenic 35.0%
TCAAATGGTCTGCAAAAACA+TGG - chr2_3:61873353-61873372 Msa0304550:intron 35.0%
GTCAGTGATGTAACATATAG+AGG - chr2_3:61873504-61873523 Msa0304550:intron 35.0%
GTAACATATAGAGGGATTAG+TGG - chr2_3:61873513-61873532 Msa0304550:intron 35.0%
TGGAACATCATTAACAGACA+AGG - chr2_3:61873533-61873552 Msa0304550:intron 35.0%
AAGGAGAAAACCTTCATCTT+AGG + chr2_3:61873636-61873655 None:intergenic 35.0%
! CAAGCAGTTTAGAATTGGTT+TGG + chr2_3:61873691-61873710 None:intergenic 35.0%
! TTGAGCAAGCAGTTTAGAAT+TGG + chr2_3:61873696-61873715 None:intergenic 35.0%
TATATTATATGGTAGCTCCG+AGG + chr2_3:61873730-61873749 None:intergenic 35.0%
TGCAACAATATTCCCTGAAA+GGG + chr2_3:61871703-61871722 None:intergenic 35.0%
TCCCAAATAAACACATGGAT+TGG - chr2_3:61871750-61871769 Msa0304550:three_prime_UTR 35.0%
AAAAATTTACTTACGGGTGG+TGG + chr2_3:61871867-61871886 None:intergenic 35.0%
GTTTCACGTTTGTAACAGAT+AGG - chr2_3:61871933-61871952 Msa0304550:CDS 35.0%
!! GTTTCGAGTTTGTTTCAGAT+AGG - chr2_3:61872022-61872041 Msa0304550:CDS 35.0%
TTGTGTGTGTATGGATGTAT+AGG - chr2_3:61872420-61872439 Msa0304550:intron 35.0%
!! TGCAGCTTGTTAGAGTTATA+TGG + chr2_3:61872513-61872532 None:intergenic 35.0%
AACTCTAACAAGCTGCAAAA+AGG - chr2_3:61872516-61872535 Msa0304550:CDS 35.0%
ACTCTAACAAGCTGCAAAAA+GGG - chr2_3:61872517-61872536 Msa0304550:CDS 35.0%
CTTAACTCTTTCATTGCAAC+TGG - chr2_3:61872628-61872647 Msa0304550:intron 35.0%
! TTGTTGTAGCATTGGATCAT+TGG - chr2_3:61872947-61872966 Msa0304550:CDS 35.0%
! TGTTGTAGCATTGGATCATT+GGG - chr2_3:61872948-61872967 Msa0304550:CDS 35.0%
ATGCACATCCTCTACAATAT+CGG + chr2_3:61872988-61873007 None:intergenic 35.0%
! ACGTTGACTGAAACATTAAC+TGG - chr2_3:61873020-61873039 Msa0304550:CDS 35.0%
AGTAAGAATCAAGACAAAGC+AGG - chr2_3:61873043-61873062 Msa0304550:CDS 35.0%
CGTACTACTACTCCTATATA+AGG - chr2_3:61873135-61873154 Msa0304550:intron 35.0%
! TATTGTATTTCTGAACAGGG+TGG - chr2_3:61873240-61873259 Msa0304550:intron 35.0%
! GTCAATCATAATGGGGTTAT+GGG + chr2_3:61873316-61873335 None:intergenic 35.0%
TCAAATGGTCTGCAAAAACA+TGG - chr2_3:61873353-61873372 Msa0304550:intron 35.0%
GTCAGTGATGTAACATATAG+AGG - chr2_3:61873504-61873523 Msa0304550:intron 35.0%
GTAACATATAGAGGGATTAG+TGG - chr2_3:61873513-61873532 Msa0304550:intron 35.0%
TGGAACATCATTAACAGACA+AGG - chr2_3:61873533-61873552 Msa0304550:intron 35.0%
AAGGAGAAAACCTTCATCTT+AGG + chr2_3:61873636-61873655 None:intergenic 35.0%
! CAAGCAGTTTAGAATTGGTT+TGG + chr2_3:61873691-61873710 None:intergenic 35.0%
! TTGAGCAAGCAGTTTAGAAT+TGG + chr2_3:61873696-61873715 None:intergenic 35.0%
TATATTATATGGTAGCTCCG+AGG + chr2_3:61873730-61873749 None:intergenic 35.0%
CAGGATCCCAAATAAACACA+TGG - chr2_3:61871745-61871764 Msa0304550:three_prime_UTR 40.0%
AGTGGAAAAGGGACTATTGA+TGG - chr2_3:61871801-61871820 Msa0304550:three_prime_UTR 40.0%
!! CTTAACGGAGGTTGATAGAA+AGG + chr2_3:61872049-61872068 None:intergenic 40.0%
CTTTCTATCAACCTCCGTTA+AGG - chr2_3:61872047-61872066 Msa0304550:CDS 40.0%
GGTTTCAGATAGGTCCTTAA+CGG + chr2_3:61872064-61872083 None:intergenic 40.0%
! TACTACTTACTGTTGGTGGA+CGG + chr2_3:61872298-61872317 None:intergenic 40.0%
TAGGTACTACTTACTGTTGG+TGG + chr2_3:61872302-61872321 None:intergenic 40.0%
GAGTAGGTACTACTTACTGT+TGG + chr2_3:61872305-61872324 None:intergenic 40.0%
GCAAGGATCAATACAAGAGT+AGG + chr2_3:61872321-61872340 None:intergenic 40.0%
CTGTTACGTACCATATGAAC+TGG - chr2_3:61872388-61872407 Msa0304550:intron 40.0%
AGAGTTATATGGCTTCTTGC+TGG + chr2_3:61872502-61872521 None:intergenic 40.0%
AACATCCGTTTAATTGCTCC+TGG - chr2_3:61872550-61872569 Msa0304550:CDS 40.0%
TCTCTCCAGGAGCAATTAAA+CGG + chr2_3:61872558-61872577 None:intergenic 40.0%
AAGCACTTGAATGTCTCTTG+AGG + chr2_3:61872611-61872630 None:intergenic 40.0%
! GATTGCATTGCTATTAGTGC+TGG - chr2_3:61872763-61872782 Msa0304550:CDS 40.0%
! GTTGTAGCATTGGATCATTG+GGG - chr2_3:61872949-61872968 Msa0304550:CDS 40.0%
! TTGTAGCATTGGATCATTGG+GGG - chr2_3:61872950-61872969 Msa0304550:CDS 40.0%
! GGTCAATCATAATGGGGTTA+TGG + chr2_3:61873317-61873336 None:intergenic 40.0%
TTTGAACTGCGACCAAAATG+TGG - chr2_3:61873563-61873582 Msa0304550:intron 40.0%
TTGAACTGCGACCAAAATGT+GGG - chr2_3:61873564-61873583 Msa0304550:intron 40.0%
ATCTGCTGTTCCTAAGATGA+AGG - chr2_3:61873623-61873642 Msa0304550:intron 40.0%
!! TAGAATTGGTTTGGTGTGTG+AGG + chr2_3:61873682-61873701 None:intergenic 40.0%
GTGCAACAATATTCCCTGAA+AGG + chr2_3:61871704-61871723 None:intergenic 40.0%
CAGGATCCCAAATAAACACA+TGG - chr2_3:61871745-61871764 Msa0304550:three_prime_UTR 40.0%
AGTGGAAAAGGGACTATTGA+TGG - chr2_3:61871801-61871820 Msa0304550:three_prime_UTR 40.0%
!! CTTAACGGAGGTTGATAGAA+AGG + chr2_3:61872049-61872068 None:intergenic 40.0%
CTTTCTATCAACCTCCGTTA+AGG - chr2_3:61872047-61872066 Msa0304550:CDS 40.0%
GGTTTCAGATAGGTCCTTAA+CGG + chr2_3:61872064-61872083 None:intergenic 40.0%
! TACTACTTACTGTTGGTGGA+CGG + chr2_3:61872298-61872317 None:intergenic 40.0%
TAGGTACTACTTACTGTTGG+TGG + chr2_3:61872302-61872321 None:intergenic 40.0%
GAGTAGGTACTACTTACTGT+TGG + chr2_3:61872305-61872324 None:intergenic 40.0%
GCAAGGATCAATACAAGAGT+AGG + chr2_3:61872321-61872340 None:intergenic 40.0%
CTGTTACGTACCATATGAAC+TGG - chr2_3:61872388-61872407 Msa0304550:intron 40.0%
AGAGTTATATGGCTTCTTGC+TGG + chr2_3:61872502-61872521 None:intergenic 40.0%
AACATCCGTTTAATTGCTCC+TGG - chr2_3:61872550-61872569 Msa0304550:CDS 40.0%
TCTCTCCAGGAGCAATTAAA+CGG + chr2_3:61872558-61872577 None:intergenic 40.0%
AAGCACTTGAATGTCTCTTG+AGG + chr2_3:61872611-61872630 None:intergenic 40.0%
! GATTGCATTGCTATTAGTGC+TGG - chr2_3:61872763-61872782 Msa0304550:CDS 40.0%
! GTTGTAGCATTGGATCATTG+GGG - chr2_3:61872949-61872968 Msa0304550:CDS 40.0%
! TTGTAGCATTGGATCATTGG+GGG - chr2_3:61872950-61872969 Msa0304550:CDS 40.0%
! GGTCAATCATAATGGGGTTA+TGG + chr2_3:61873317-61873336 None:intergenic 40.0%
TTTGAACTGCGACCAAAATG+TGG - chr2_3:61873563-61873582 Msa0304550:intron 40.0%
TTGAACTGCGACCAAAATGT+GGG - chr2_3:61873564-61873583 Msa0304550:intron 40.0%
ATCTGCTGTTCCTAAGATGA+AGG - chr2_3:61873623-61873642 Msa0304550:intron 40.0%
!! TAGAATTGGTTTGGTGTGTG+AGG + chr2_3:61873682-61873701 None:intergenic 40.0%
! AAAGGGACTATTGATGGTCG+AGG - chr2_3:61871807-61871826 Msa0304550:three_prime_UTR 45.0%
TGGTGGAAACAACCTTGCAT+AGG - chr2_3:61871837-61871856 Msa0304550:exon 45.0%
! TTTAAGGCCAAATACCTCCC+TGG - chr2_3:61871901-61871920 Msa0304550:CDS 45.0%
TTAAGGCCAAATACCTCCCT+GGG - chr2_3:61871902-61871921 Msa0304550:CDS 45.0%
CGTGAAACTTAAAGGACCCA+GGG + chr2_3:61872144-61872163 None:intergenic 45.0%
ACGTGAAACTTAAAGGACCC+AGG + chr2_3:61872143-61872162 None:intergenic 45.0%
TTCAGATAGGTCCTTAACGG+AGG + chr2_3:61872061-61872080 None:intergenic 45.0%
ACGTGAAACTTAAAGGACCC+AGG - chr2_3:61872143-61872162 Msa0304550:intron 45.0%
CGTGAAACTTAAAGGACCCA+GGG - chr2_3:61872144-61872163 Msa0304550:intron 45.0%
AAAAGGCCAAATACCTCCCT+GGG + chr2_3:61872163-61872182 None:intergenic 45.0%
AAAAAGGCCAAATACCTCCC+TGG + chr2_3:61872164-61872183 None:intergenic 45.0%
ACTGCTATAACATGTGGTCC+AGG - chr2_3:61872805-61872824 Msa0304550:intron 45.0%
ATAACATGTGGTCCAGGACA+TGG - chr2_3:61872811-61872830 Msa0304550:intron 45.0%
ACTAACCTGATACCATGTCC+TGG + chr2_3:61872826-61872845 None:intergenic 45.0%
TGGAGACACCGATATTGTAG+AGG - chr2_3:61872977-61872996 Msa0304550:CDS 45.0%
! TGTATTTCTGAACAGGGTGG+AGG - chr2_3:61873243-61873262 Msa0304550:intron 45.0%
TGAACTGCGACCAAAATGTG+GGG - chr2_3:61873565-61873584 Msa0304550:intron 45.0%
TCCTTCTGCCATAATGCTCA+TGG - chr2_3:61873651-61873670 Msa0304550:intron 45.0%
TCCATGAGCATTATGGCAGA+AGG + chr2_3:61873655-61873674 None:intergenic 45.0%
AGGCTCTTCCATGAGCATTA+TGG + chr2_3:61873662-61873681 None:intergenic 45.0%
! AAAGGGACTATTGATGGTCG+AGG - chr2_3:61871807-61871826 Msa0304550:three_prime_UTR 45.0%
TGGTGGAAACAACCTTGCAT+AGG - chr2_3:61871837-61871856 Msa0304550:exon 45.0%
! TTTAAGGCCAAATACCTCCC+TGG - chr2_3:61871901-61871920 Msa0304550:CDS 45.0%
TTAAGGCCAAATACCTCCCT+GGG - chr2_3:61871902-61871921 Msa0304550:CDS 45.0%
CGTGAAACTTAAAGGACCCA+GGG + chr2_3:61872144-61872163 None:intergenic 45.0%
ACGTGAAACTTAAAGGACCC+AGG + chr2_3:61872143-61872162 None:intergenic 45.0%
TTCAGATAGGTCCTTAACGG+AGG + chr2_3:61872061-61872080 None:intergenic 45.0%
ACGTGAAACTTAAAGGACCC+AGG - chr2_3:61872143-61872162 Msa0304550:intron 45.0%
CGTGAAACTTAAAGGACCCA+GGG - chr2_3:61872144-61872163 Msa0304550:intron 45.0%
AAAAGGCCAAATACCTCCCT+GGG + chr2_3:61872163-61872182 None:intergenic 45.0%
AAAAAGGCCAAATACCTCCC+TGG + chr2_3:61872164-61872183 None:intergenic 45.0%
ACTGCTATAACATGTGGTCC+AGG - chr2_3:61872805-61872824 Msa0304550:intron 45.0%
ATAACATGTGGTCCAGGACA+TGG - chr2_3:61872811-61872830 Msa0304550:intron 45.0%
ACTAACCTGATACCATGTCC+TGG + chr2_3:61872826-61872845 None:intergenic 45.0%
TGGAGACACCGATATTGTAG+AGG - chr2_3:61872977-61872996 Msa0304550:CDS 45.0%
! TGTATTTCTGAACAGGGTGG+AGG - chr2_3:61873243-61873262 Msa0304550:intron 45.0%
TGAACTGCGACCAAAATGTG+GGG - chr2_3:61873565-61873584 Msa0304550:intron 45.0%
TCCTTCTGCCATAATGCTCA+TGG - chr2_3:61873651-61873670 Msa0304550:intron 45.0%
TCCATGAGCATTATGGCAGA+AGG + chr2_3:61873655-61873674 None:intergenic 45.0%
AGGCTCTTCCATGAGCATTA+TGG + chr2_3:61873662-61873681 None:intergenic 45.0%
! TTGATGGTCGAGGCTCTATG+TGG - chr2_3:61871817-61871836 Msa0304550:three_prime_UTR 50.0%
!! GGTGGTGGATTTCCTATGCA+AGG + chr2_3:61871852-61871871 None:intergenic 50.0%
!! AAAGGACCCAGGGAGGTATT+TGG + chr2_3:61872154-61872173 None:intergenic 50.0%
GAAACTTAAAGGACCCAGGG+AGG + chr2_3:61872147-61872166 None:intergenic 50.0%
GAAACTTAAAGGACCCAGGG+AGG - chr2_3:61872147-61872166 Msa0304550:intron 50.0%
!! AAAGGACCCAGGGAGGTATT+TGG - chr2_3:61872154-61872173 Msa0304550:intron 50.0%
GGCAACTTGTCCCCTGAAAA+AGG + chr2_3:61872277-61872296 None:intergenic 50.0%
TCAGTATTAGGGCTCTCTCC+AGG + chr2_3:61872571-61872590 None:intergenic 50.0%
GGAGAGAGCCCTAATACTGA+TGG - chr2_3:61872571-61872590 Msa0304550:CDS 50.0%
ATTTCTGAACAGGGTGGAGG+TGG - chr2_3:61873246-61873265 Msa0304550:intron 50.0%
!! GGTTAGAGCACCCCACATTT+TGG + chr2_3:61873578-61873597 None:intergenic 50.0%
GCTCAAGTAGCTAGCTACCT+CGG - chr2_3:61873710-61873729 Msa0304550:intron 50.0%
! TTGATGGTCGAGGCTCTATG+TGG - chr2_3:61871817-61871836 Msa0304550:three_prime_UTR 50.0%
!! GGTGGTGGATTTCCTATGCA+AGG + chr2_3:61871852-61871871 None:intergenic 50.0%
!! AAAGGACCCAGGGAGGTATT+TGG + chr2_3:61872154-61872173 None:intergenic 50.0%
GAAACTTAAAGGACCCAGGG+AGG + chr2_3:61872147-61872166 None:intergenic 50.0%
GAAACTTAAAGGACCCAGGG+AGG - chr2_3:61872147-61872166 Msa0304550:intron 50.0%
!! AAAGGACCCAGGGAGGTATT+TGG - chr2_3:61872154-61872173 Msa0304550:intron 50.0%
GGCAACTTGTCCCCTGAAAA+AGG + chr2_3:61872277-61872296 None:intergenic 50.0%
TCAGTATTAGGGCTCTCTCC+AGG + chr2_3:61872571-61872590 None:intergenic 50.0%
GGAGAGAGCCCTAATACTGA+TGG - chr2_3:61872571-61872590 Msa0304550:CDS 50.0%
ATTTCTGAACAGGGTGGAGG+TGG - chr2_3:61873246-61873265 Msa0304550:intron 50.0%
!! GGTTAGAGCACCCCACATTT+TGG + chr2_3:61873578-61873597 None:intergenic 50.0%
GCTCAAGTAGCTAGCTACCT+CGG - chr2_3:61873710-61873729 Msa0304550:intron 50.0%
ATGGTCGAGGCTCTATGTGG+TGG - chr2_3:61871820-61871839 Msa0304550:exon 55.0%
GTGGTCCAGGACATGGTATC+AGG - chr2_3:61872818-61872837 Msa0304550:intron 55.0%
! ATTGGATCATTGGGGGCACG+TGG - chr2_3:61872957-61872976 Msa0304550:CDS 55.0%
! AGGGTGGAGGTGGATTTGCT+AGG - chr2_3:61873256-61873275 Msa0304550:intron 55.0%
!! GTGGAGGTGGATTTGCTAGG+AGG - chr2_3:61873259-61873278 Msa0304550:intron 55.0%
ATGGTCGAGGCTCTATGTGG+TGG - chr2_3:61871820-61871839 Msa0304550:exon 55.0%
GTGGTCCAGGACATGGTATC+AGG - chr2_3:61872818-61872837 Msa0304550:intron 55.0%
! ATTGGATCATTGGGGGCACG+TGG - chr2_3:61872957-61872976 Msa0304550:CDS 55.0%
! AGGGTGGAGGTGGATTTGCT+AGG - chr2_3:61873256-61873275 Msa0304550:intron 55.0%
!! GTGGAGGTGGATTTGCTAGG+AGG - chr2_3:61873259-61873278 Msa0304550:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 61871702 61873855 61871702 ID=Msa0304550;Name=Msa0304550
chr2_3 mRNA 61871702 61873855 61871702 ID=Msa0304550-mRNA-1;Parent=Msa0304550;Name=Msa0304550-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.66|0.71|1|0.66|0.85|7|136|296
chr2_3 exon 61871702 61872068 61871702 ID=Msa0304550-mRNA-1:exon:20593;Parent=Msa0304550-mRNA-1
chr2_3 exon 61872183 61872299 61872183 ID=Msa0304550-mRNA-1:exon:20592;Parent=Msa0304550-mRNA-1
chr2_3 exon 61872493 61872601 61872493 ID=Msa0304550-mRNA-1:exon:20591;Parent=Msa0304550-mRNA-1
chr2_3 exon 61872718 61872799 61872718 ID=Msa0304550-mRNA-1:exon:20590;Parent=Msa0304550-mRNA-1
chr2_3 exon 61872908 61873115 61872908 ID=Msa0304550-mRNA-1:exon:20589;Parent=Msa0304550-mRNA-1
chr2_3 exon 61873267 61873273 61873267 ID=Msa0304550-mRNA-1:exon:20588;Parent=Msa0304550-mRNA-1
chr2_3 exon 61873719 61873855 61873719 ID=Msa0304550-mRNA-1:exon:20587;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61873719 61873855 61873719 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61873267 61873273 61873267 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61872908 61873115 61872908 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61872718 61872799 61872718 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61872493 61872601 61872493 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61872183 61872299 61872183 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 CDS 61871838 61872068 61871838 ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3 three_prime_UTR 61871702 61871837 61871702 ID=Msa0304550-mRNA-1:three_prime_utr;Parent=Msa0304550-mRNA-1
Gene Sequence

>Msa0304550

CTTTCAGGGAATATTGTTGCACCTAAAACAAAATCGGAATATTCAGGATCCCAAATAAACACATGGATTGGTTTCTCATTTGTGAATGGTTTGATTATAAGTGGAAAAGGGACTATTGATGGTCGAGGCTCTATGTGGGGACAAGCAATAACATTGAATAGATGCTATCGATTTCAAATAAAAGGATATACAAGTGTTAATCCAGCAAGAAGCCATATAACTCTAACAAGCTGCAAAAAGGGCATTATCTCTAACATCCGTTTAATTGCTCCTGGAGAGAGCCCTAATACTGATGGCATTGATATTTCTGCCTCAAGAGACATTCAAGTGCTTAACTCTTTCATTGCAACTGGTGATGATTGCATTGCTATTAGTGCTGGATCATCAGTAATCAAAATAACTGCTATAACATGTGGTCCAGGACATGGTATCAGCATTGGATCATTGGGGGCACGTGGAGACACCGATATTGTAGAGGATGTGCATGTGAAGAATTGTACGTTGACTGAAACATTAACTGGAGTAAGAATCAAGACAAAGCAGGGTGGAGGTGGATTTGCTAGGAGGATCACGTTTGAAAATATTAGATTTGTTCGAGCCCATAACCCCATTATGATTGACCAATTTTATTGTGTTAATCAAATGGTCTGCAAAAACATGACTAAAGCAATCAAAGTCAGTGATGTAACATATAGAGGGATTAGTGGAACATCATTAACAGACAAGGCAATAAATTTGAACTGCGACCAAAATGTGGGGTGCTCTAACCTTGTATTTGATCGTGTTTATGTAAGATCTGCTGTTCCTAAGATGAAGGTTTTCTCCTTCTGCCATAATGCTCATGGAAGAGCCTCACACACCAAACCAATTCTAAACTGCTTGCTCAAGTAG
Protein sequence

>Msa0304550

LSGNIVAPKTKSEYSGSQINTWIGFSFVNGLIISGKGTIDGRGSMWGQAITLNRCYRFQIKGYTSVNPARSHITLTSCKKGIISNIRLIAPGESPNTDGIDISASRDIQVLNSFIATGDDCIAISAGSSVIKITAITCGPGHGISIGSLGARGDTDIVEDVHVKNCTLTETLTGVRIKTKQGGGGFARRITFENIRFVRAHNPIMIDQFYCVNQMVCKNMTKAIKVSDVTYRGISGTSLTDKAINLNCDQNVGCSNLVFDRVYVRSAVPKMKVFSFCHNAHGRASHTKPILNCLLK*