AlfalfaGEDB | Msa0304550

M. sativa cultivar zhongmu-4 / Msa0304550

KEGG: K01184
GO: -
PFAM: PF00295.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304550	QKO47089.1	93.291	313	4	1	1	296	92	404	0.0	584

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304550	sp\|Q94AJ5\|PGLR5_ARATH	36.877	301	174	1	1	285	115	415	2.56e-66	216

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304550	A0A6N0D4P9	93.291	313	4	1	1	296	92	404	0.0	584

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012780	Msa0304550	0.820581	6.360888e-53	-8.615850e-47
Msa0012790	Msa0304550	0.834393	3.076422e-56	-8.615850e-47
Msa0048950	Msa0304550	0.820837	5.554374e-53	-8.615850e-47
Msa0059880	Msa0304550	0.800894	1.163544e-48	-8.615850e-47
Msa0061590	Msa0304550	0.823122	1.641597e-53	-8.615850e-47
Msa0072030	Msa0304550	0.823253	1.530376e-53	-8.615850e-47
Msa0089010	Msa0304550	-0.819352	1.214748e-52	-8.615850e-47
Msa0106110	Msa0304550	0.827469	1.537935e-54	-8.615850e-47
Msa0131460	Msa0304550	0.818026	2.428975e-52	-8.615850e-47
Msa0152640	Msa0304550	0.834308	3.231864e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304550	MtrunA17_Chr2g0332971	91.693	313	9	1	1	296	92	404	0.0	578
Msa0304550	MtrunA17_Chr5g0418411	44.625	307	154	5	1	294	93	396	6.22e-81	250
Msa0304550	MtrunA17_Chr8g0337571	44.408	304	150	5	1	294	94	388	2.47e-79	245
Msa0304550	MtrunA17_Chr3g0100091	43.464	306	154	4	1	294	48	346	3.83e-79	244
Msa0304550	MtrunA17_Chr3g0121891	44.839	310	148	5	1	294	93	395	4.97e-79	245
Msa0304550	MtrunA17_Chr4g0043661	42.258	310	161	5	1	294	87	394	4.35e-78	242
Msa0304550	MtrunA17_Chr3g0121881	41.967	305	164	4	1	294	97	399	2.28e-77	241
Msa0304550	MtrunA17_Chr4g0018151	46.358	302	151	5	1	294	142	440	4.12e-73	231
Msa0304550	MtrunA17_Chr2g0332801	40.268	298	161	2	3	284	123	419	2.55e-71	227
Msa0304550	MtrunA17_Chr6g0461181	37.464	347	158	5	1	294	94	434	8.99e-71	225

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304550	AT5G17200.1	45.342	322	144	5	1	294	102	419	1.25e-84	260
Msa0304550	AT5G27530.1	44.781	297	157	2	1	294	117	409	1.95e-80	251
Msa0304550	AT5G44830.1	42.517	294	167	2	1	294	39	330	7.55e-78	240
Msa0304550	AT4G32380.1	42.123	292	158	2	1	281	91	382	2.75e-77	243
Msa0304550	AT4G35670.1	43.322	307	155	4	1	294	88	388	8.02e-73	229
Msa0304550	AT5G44840.1	39.145	304	173	4	1	294	88	389	1.53e-70	223
Msa0304550	AT4G35670.2	43.706	286	142	4	22	294	2	281	3.78e-70	219
Msa0304550	AT1G80170.1	36.877	301	174	1	1	285	115	415	2.60e-67	216
Msa0304550	AT3G15720.4	39.274	303	171	5	3	294	25	325	1.28e-66	213
Msa0304550	AT4G32375.1	40.647	278	155	2	13	280	4	281	2.83e-66	213

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Find 71 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAATCGGAATATTC+AGG	0.348846	2_3:-61873809	Msa0304550:CDS
AAAATAACTGCTATAACATG+TGG	0.629212	2_3:-61872736	Msa0304550:CDS
AAAATTGGTCAATCATAATG+GGG	0.641528	2_3:+61872215	None:intergenic
AAAGACACGGTAATCGTACA+CGG	0.682275	2_3:+61871694	None:intergenic
AAAGGGACTATTGATGGTCG+AGG	0.592324	2_3:-61873728	Msa0304550:CDS
AACATCCGTTTAATTGCTCC+TGG	0.419774	2_3:-61872985	Msa0304550:CDS
AACCAATCCATGTGTTTATT+TGG	0.080796	2_3:+61873783	None:intergenic
AACTCTAACAAGCTGCAAAA+AGG	0.263487	2_3:-61873019	Msa0304550:CDS
AAGCACTTGAATGTCTCTTG+AGG	0.634460	2_3:+61872927	None:intergenic
AAGGAGAAAACCTTCATCTT+AGG	0.335531	2_3:+61871902	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTGCACCTAAAACAAAAT+CGG	-	chr2_3:61871716-61871735	Msa0304550:three_prime_UTR	30.0%
ACCAATCCATGTGTTTATTT+GGG	+	chr2_3:61871754-61871773	None:intergenic	30.0%
AACCAATCCATGTGTTTATT+TGG	+	chr2_3:61871755-61871774	None:intergenic	30.0%
GTGAATGGTTTGATTATAAG+TGG	-	chr2_3:61871783-61871802	Msa0304550:three_prime_UTR	30.0%
GGTTTGATTATAAGTGGAAA+AGG	-	chr2_3:61871789-61871808	Msa0304550:three_prime_UTR	30.0%
GTTACAAACGTGAAACTTAA+AGG	+	chr2_3:61872136-61872155	None:intergenic	30.0%
CTGAAACCTTAGAAACTTAA+AGG	+	chr2_3:61871989-61872008	None:intergenic	30.0%
GAAACAAACTCGAAACTTAA+AGG	+	chr2_3:61872018-61872037	None:intergenic	30.0%
GTTACAAACGTGAAACTTAA+AGG	-	chr2_3:61872136-61872155	Msa0304550:intron	30.0%
TATCAATGCCATCAGTATTA+GGG	+	chr2_3:61872582-61872601	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	61871702	61873855	61871702	ID=Msa0304550;Name=Msa0304550
chr2_3	mRNA	61871702	61873855	61871702	ID=Msa0304550-mRNA-1;Parent=Msa0304550;Name=Msa0304550-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0.66\|0.71\|1\|0.66\|0.85\|7\|136\|296
chr2_3	exon	61871702	61872068	61871702	ID=Msa0304550-mRNA-1:exon:20593;Parent=Msa0304550-mRNA-1
chr2_3	exon	61872183	61872299	61872183	ID=Msa0304550-mRNA-1:exon:20592;Parent=Msa0304550-mRNA-1
chr2_3	exon	61872493	61872601	61872493	ID=Msa0304550-mRNA-1:exon:20591;Parent=Msa0304550-mRNA-1
chr2_3	exon	61872718	61872799	61872718	ID=Msa0304550-mRNA-1:exon:20590;Parent=Msa0304550-mRNA-1
chr2_3	exon	61872908	61873115	61872908	ID=Msa0304550-mRNA-1:exon:20589;Parent=Msa0304550-mRNA-1
chr2_3	exon	61873267	61873273	61873267	ID=Msa0304550-mRNA-1:exon:20588;Parent=Msa0304550-mRNA-1
chr2_3	exon	61873719	61873855	61873719	ID=Msa0304550-mRNA-1:exon:20587;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61873719	61873855	61873719	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61873267	61873273	61873267	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61872908	61873115	61872908	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61872718	61872799	61872718	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61872493	61872601	61872493	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61872183	61872299	61872183	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	CDS	61871838	61872068	61871838	ID=Msa0304550-mRNA-1:cds;Parent=Msa0304550-mRNA-1
chr2_3	three_prime_UTR	61871702	61871837	61871702	ID=Msa0304550-mRNA-1:three_prime_utr;Parent=Msa0304550-mRNA-1

Gene Sequence

>Msa0304550

CTTTCAGGGAATATTGTTGCACCTAAAACAAAATCGGAATATTCAGGATCCCAAATAAACACATGGATTGGTTTCTCATTTGTGAATGGTTTGATTATAAGTGGAAAAGGGACTATTGATGGTCGAGGCTCTATGTGGGGACAAGCAATAACATTGAATAGATGCTATCGATTTCAAATAAAAGGATATACAAGTGTTAATCCAGCAAGAAGCCATATAACTCTAACAAGCTGCAAAAAGGGCATTATCTCTAACATCCGTTTAATTGCTCCTGGAGAGAGCCCTAATACTGATGGCATTGATATTTCTGCCTCAAGAGACATTCAAGTGCTTAACTCTTTCATTGCAACTGGTGATGATTGCATTGCTATTAGTGCTGGATCATCAGTAATCAAAATAACTGCTATAACATGTGGTCCAGGACATGGTATCAGCATTGGATCATTGGGGGCACGTGGAGACACCGATATTGTAGAGGATGTGCATGTGAAGAATTGTACGTTGACTGAAACATTAACTGGAGTAAGAATCAAGACAAAGCAGGGTGGAGGTGGATTTGCTAGGAGGATCACGTTTGAAAATATTAGATTTGTTCGAGCCCATAACCCCATTATGATTGACCAATTTTATTGTGTTAATCAAATGGTCTGCAAAAACATGACTAAAGCAATCAAAGTCAGTGATGTAACATATAGAGGGATTAGTGGAACATCATTAACAGACAAGGCAATAAATTTGAACTGCGACCAAAATGTGGGGTGCTCTAACCTTGTATTTGATCGTGTTTATGTAAGATCTGCTGTTCCTAAGATGAAGGTTTTCTCCTTCTGCCATAATGCTCATGGAAGAGCCTCACACACCAAACCAATTCTAAACTGCTTGCTCAAGTAG

Protein sequence

>Msa0304550

LSGNIVAPKTKSEYSGSQINTWIGFSFVNGLIISGKGTIDGRGSMWGQAITLNRCYRFQIKGYTSVNPARSHITLTSCKKGIISNIRLIAPGESPNTDGIDISASRDIQVLNSFIATGDDCIAISAGSSVIKITAITCGPGHGISIGSLGARGDTDIVEDVHVKNCTLTETLTGVRIKTKQGGGGFARRITFENIRFVRAHNPIMIDQFYCVNQMVCKNMTKAIKVSDVTYRGISGTSLTDKAINLNCDQNVGCSNLVFDRVYVRSAVPKMKVFSFCHNAHGRASHTKPILNCLLK*