AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0341110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0341110 MtrunA17_Chr2g0332971 91.111 315 11 1 1 298 90 404 0.0 580
Msa0341110 MtrunA17_Chr5g0418411 44.337 309 156 5 1 296 91 396 3.97e-81 250
Msa0341110 MtrunA17_Chr8g0337571 44.118 306 152 5 1 296 92 388 1.70e-79 246
Msa0341110 MtrunA17_Chr3g0100091 43.464 306 154 4 3 296 48 346 3.16e-79 244
Msa0341110 MtrunA17_Chr3g0121891 44.551 312 150 5 1 296 91 395 3.31e-79 246
Msa0341110 MtrunA17_Chr4g0043661 41.987 312 163 5 1 296 85 394 3.19e-78 243
Msa0341110 MtrunA17_Chr3g0121881 41.694 307 166 4 1 296 95 399 2.10e-77 241
Msa0341110 MtrunA17_Chr4g0018151 46.053 304 153 5 1 296 140 440 4.18e-73 231
Msa0341110 MtrunA17_Chr2g0332801 39.735 302 165 2 1 286 119 419 2.11e-71 227
Msa0341110 MtrunA17_Chr6g0461181 37.249 349 160 5 1 296 92 434 5.63e-71 226
Msa0341110 MtrunA17_Chr8g0336481 44.444 261 127 5 1 250 14 267 5.27e-69 215
Msa0341110 MtrunA17_Chr8g0337581 41.368 307 159 7 1 296 93 389 4.60e-68 216
Msa0341110 MtrunA17_Chr7g0228411 40.299 335 153 8 1 295 102 429 5.47e-67 215
Msa0341110 MtrunA17_Chr8g0365901 38.000 300 168 2 1 284 124 421 1.60e-66 214
Msa0341110 MtrunA17_Chr8g0337511 44.068 236 125 3 50 285 3 231 6.67e-64 201
Msa0341110 MtrunA17_Chr8g0337601 44.444 243 129 2 54 296 4 240 2.51e-63 199
Msa0341110 MtrunA17_Chr1g0173451 39.560 273 155 2 1 264 135 406 8.30e-63 205
Msa0341110 MtrunA17_Chr6g0449961 37.742 310 176 7 1 298 105 409 5.62e-61 199
Msa0341110 MtrunA17_Chr1g0150291 36.333 300 172 4 1 287 107 400 4.98e-60 196
Msa0341110 MtrunA17_Chr4g0020771 37.809 283 156 2 1 264 131 412 6.86e-59 194
Msa0341110 MtrunA17_Chr1g0150301 34.667 300 177 4 1 287 89 382 1.19e-57 190
Msa0341110 MtrunA17_Chr1g0194231 34.472 322 185 5 1 297 142 462 1.84e-55 186
Msa0341110 MtrunA17_Chr5g0413141 32.792 308 183 4 1 286 118 423 1.30e-54 184
Msa0341110 MtrunA17_Chr7g0230891 33.987 306 183 6 5 296 100 400 2.53e-54 181
Msa0341110 MtrunA17_Chr3g0089021 40.071 282 152 2 1 266 129 409 4.76e-54 181
Msa0341110 MtrunA17_Chr4g0052621 32.710 321 190 5 1 296 124 443 4.99e-54 182
Msa0341110 MtrunA17_Chr8g0365891 31.965 341 184 7 1 296 167 504 1.98e-53 182
Msa0341110 MtrunA17_Chr5g0413411 31.765 340 185 7 1 296 181 517 3.88e-53 181
Msa0341110 MtrunA17_Chr7g0270361 32.298 322 193 5 1 298 128 448 1.05e-52 179
Msa0341110 MtrunA17_Chr2g0291841 35.144 313 176 9 3 296 58 362 1.38e-51 173
Msa0341110 MtrunA17_Chr4g0043431 39.453 256 139 4 24 264 116 370 1.06e-50 172
Msa0341110 MtrunA17_Chr2g0303941 37.458 299 162 9 3 284 89 379 1.72e-50 171
Msa0341110 MtrunA17_Chr1g0195221 37.276 279 153 6 14 275 110 383 2.27e-50 171
Msa0341110 MtrunA17_Chr2g0307811 36.014 286 160 5 3 267 84 367 4.01e-50 171
Msa0341110 MtrunA17_Chr2g0307831 35.069 288 164 5 1 267 82 367 1.01e-48 167
Msa0341110 MtrunA17_Chr2g0307821 35.069 288 164 5 1 267 82 367 1.01e-48 167
Msa0341110 MtrunA17_Chr3g0093011 35.417 240 152 1 47 283 9 248 5.51e-48 161
Msa0341110 MtrunA17_Chr7g0257701 40.086 232 128 3 1 222 160 390 7.42e-48 164
Msa0341110 MtrunA17_Chr4g0067631 36.042 283 157 5 5 267 117 395 2.73e-46 161
Msa0341110 MtrunA17_Chr2g0293681 30.938 320 163 7 1 296 126 411 1.52e-44 156
Msa0341110 MtrunA17_Chr8g0348981 31.615 291 181 3 24 296 108 398 2.30e-44 155
Msa0341110 MtrunA17_Chr8g0337351 42.690 171 97 1 62 231 1 171 9.89e-40 137
Msa0341110 MtrunA17_Chr8g0337551 48.485 132 68 0 50 181 3 134 1.62e-38 133
Msa0341110 MtrunA17_Chr1g0149241 30.769 312 161 6 1 296 90 362 3.39e-38 138
Msa0341110 MtrunA17_Chr5g0425811 34.454 238 129 5 3 219 41 272 6.54e-38 135
Msa0341110 MtrunA17_Chr7g0257721 39.086 197 103 2 1 181 135 330 4.35e-37 134
Msa0341110 MtrunA17_Chr5g0447901 35.498 231 130 6 1 213 82 311 2.27e-36 132
Msa0341110 MtrunA17_Chr8g0337521 40.314 191 95 4 1 181 92 273 3.33e-35 129
Msa0341110 MtrunA17_Chr2g0295451 36.564 227 122 5 5 212 118 341 4.61e-32 122
Msa0341110 MtrunA17_Chr7g0273191 44.348 115 64 0 51 165 24 138 4.43e-29 108
Msa0341110 MtrunA17_Chr8g0337561 42.609 115 60 2 184 298 8 116 2.99e-22 89.7
Msa0341110 MtrunA17_Chr8g0337531 39.231 130 72 3 167 296 9 131 3.21e-21 87.4
Msa0341110 MtrunA17_Chr7g0270821 26.582 316 175 11 26 290 143 452 1.24e-20 91.7
Msa0341110 MtrunA17_Chr7g0255311 31.100 209 113 7 18 199 154 358 8.55e-19 86.3
Msa0341110 MtrunA17_Chr2g0301091 27.152 302 182 10 15 286 144 437 6.65e-18 83.6
Msa0341110 MtrunA17_Chr4g0070281 26.159 302 185 9 15 286 163 456 9.95e-18 83.2
Msa0341110 MtrunA17_Chr3g0130251 29.808 208 107 6 30 206 179 378 3.08e-17 81.6
Msa0341110 MtrunA17_Chr1g0202641 30.412 194 105 6 25 192 154 343 3.25e-17 82.0
Msa0341110 MtrunA17_Chr1g0202641 26.403 303 172 11 25 291 654 941 1.14e-16 80.5
Msa0341110 MtrunA17_Chr8g0387391 25.651 269 151 8 25 248 143 407 1.20e-16 79.7
Msa0341110 MtrunA17_Chr1g0202651 29.897 194 106 6 25 192 154 343 1.24e-16 79.7
Msa0341110 MtrunA17_Chr8g0373171 40.230 87 52 0 50 136 5 91 2.20e-15 71.2
Msa0341110 MtrunA17_Chr1g0193961 27.103 214 123 4 26 212 143 350 6.61e-15 74.7
Msa0341110 MtrunA17_Chr1g0202621 24.444 270 157 11 25 253 147 410 7.53e-15 74.7
Msa0341110 MtrunA17_Chr3g0095221 30.693 202 103 7 28 197 143 339 1.78e-14 73.6
Msa0341110 MtrunA17_Chr5g0442221 28.972 214 115 7 27 208 140 348 7.20e-14 71.6
Msa0341110 MtrunA17_Chr8g0348491 24.232 293 191 6 25 291 154 441 4.03e-13 69.3
Msa0341110 MtrunA17_Chr7g0248451 29.897 194 106 6 25 192 154 343 4.21e-13 69.3
Msa0341110 MtrunA17_Chr7g0273181 35.714 84 53 1 204 287 6 88 6.29e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0341110 AT5G17200.1 45.846 325 142 7 1 296 100 419 2.92e-85 262
Msa0341110 AT5G27530.1 44.482 299 159 2 1 296 115 409 5.69e-81 252
Msa0341110 AT5G44830.1 42.230 296 169 2 1 296 37 330 4.41e-78 241
Msa0341110 AT4G32380.1 41.837 294 160 2 1 283 89 382 2.43e-77 243
Msa0341110 AT4G35670.1 43.042 309 157 4 1 296 86 388 7.23e-73 229
Msa0341110 AT5G44840.1 38.889 306 175 4 1 296 86 389 1.18e-70 224
Msa0341110 AT4G35670.2 43.706 286 142 4 24 296 2 281 5.08e-70 219
Msa0341110 AT1G80170.1 36.634 303 176 1 1 287 113 415 1.09e-67 218
Msa0341110 AT3G15720.4 38.762 307 175 5 1 296 21 325 1.23e-66 213
Msa0341110 AT3G15720.2 39.474 304 174 5 1 296 84 385 2.64e-66 214
Msa0341110 AT4G32375.1 40.647 278 155 2 15 282 4 281 2.99e-66 213
Msa0341110 AT3G15720.3 38.762 307 175 5 1 296 84 388 5.82e-66 213
Msa0341110 AT3G15720.1 38.762 307 175 5 1 296 85 389 5.90e-66 213
Msa0341110 AT1G70500.1 37.667 300 169 2 1 284 126 423 9.34e-65 211
Msa0341110 AT1G56710.1 37.812 320 175 3 1 297 110 428 1.15e-64 209
Msa0341110 AT1G23460.2 37.667 300 169 2 1 284 102 399 9.04e-63 204
Msa0341110 AT1G23460.1 37.061 313 178 3 1 296 126 436 1.53e-62 204
Msa0341110 AT4G32380.2 36.644 292 137 2 1 283 89 341 8.43e-62 201
Msa0341110 AT3G57510.1 39.583 288 153 4 5 274 132 416 1.87e-61 201
Msa0341110 AT5G14650.1 37.540 309 175 7 3 296 113 418 3.05e-60 198
Msa0341110 AT2G41850.1 40.569 281 146 4 5 267 132 409 7.47e-58 192
Msa0341110 AT4G01890.1 35.127 316 177 6 8 296 134 448 2.51e-56 189
Msa0341110 AT1G48100.1 33.540 322 188 6 1 297 139 459 2.56e-56 189
Msa0341110 AT1G80140.1 36.156 307 181 5 3 296 32 336 2.61e-56 185
Msa0341110 AT1G10640.1 33.136 338 179 6 3 296 181 515 7.06e-56 189
Msa0341110 AT3G07970.1 37.500 280 157 2 1 264 131 408 2.12e-55 186
Msa0341110 AT2G43880.1 37.540 309 168 7 5 296 94 394 1.33e-54 182
Msa0341110 AT1G02460.1 35.185 324 182 8 1 297 151 473 2.20e-54 184
Msa0341110 AT4G32370.1 41.048 229 122 3 1 216 98 326 2.75e-54 180
Msa0341110 AT1G60590.1 33.724 341 176 8 3 296 185 522 5.92e-54 184
Msa0341110 AT1G05650.1 36.545 301 169 8 1 285 87 381 1.07e-53 180
Msa0341110 AT1G05660.1 34.936 312 181 8 1 296 87 392 2.73e-53 179
Msa0341110 AT3G26610.1 34.516 310 176 6 1 285 126 433 2.79e-53 181
Msa0341110 AT1G65570.1 37.124 299 169 7 1 285 93 386 2.93e-53 179
Msa0341110 AT2G43860.1 36.334 311 177 7 3 298 96 400 1.06e-51 175
Msa0341110 AT2G43890.1 37.079 267 149 6 5 256 92 354 3.64e-50 171
Msa0341110 AT5G48140.1 36.742 264 144 4 24 267 98 358 3.77e-50 171
Msa0341110 AT4G18180.1 36.882 263 143 3 24 266 122 381 6.96e-50 171
Msa0341110 AT3G07820.1 35.606 264 147 4 24 267 98 358 5.22e-48 165
Msa0341110 AT3G59850.1 37.050 278 154 8 3 264 86 358 1.31e-47 164
Msa0341110 AT2G26620.1 32.459 305 179 5 3 286 93 391 1.85e-47 164
Msa0341110 AT2G40310.1 31.875 320 187 7 1 296 91 403 1.91e-47 164
Msa0341110 AT1G17150.1 32.484 314 190 7 1 296 92 401 2.23e-47 164
Msa0341110 AT3G59850.2 37.050 278 154 8 3 264 130 402 2.28e-47 164
Msa0341110 AT1G02790.1 36.719 256 141 5 17 256 122 372 9.71e-47 162
Msa0341110 AT3G07830.1 33.221 298 168 5 24 293 99 393 9.88e-47 162
Msa0341110 AT1G78400.1 35.036 274 158 5 3 259 96 366 1.08e-46 162
Msa0341110 AT1G43080.1 31.875 320 187 7 1 296 91 403 1.45e-46 161
Msa0341110 AT2G15460.1 33.443 305 178 8 3 286 93 393 2.55e-46 160
Msa0341110 AT4G13760.1 33.929 280 155 6 3 259 93 365 4.28e-46 160
Msa0341110 AT2G43870.1 34.727 311 176 12 3 296 84 384 6.50e-46 159
Msa0341110 AT1G43090.1 32.468 308 177 7 1 284 91 391 8.26e-46 160
Msa0341110 AT1G43100.1 32.468 308 177 7 1 284 91 391 8.43e-46 160
Msa0341110 AT2G15450.1 33.115 305 179 8 3 286 93 393 1.05e-45 159
Msa0341110 AT2G15470.1 33.115 305 179 8 3 286 93 393 1.35e-45 159
Msa0341110 AT3G07850.1 35.463 313 170 8 1 293 127 427 2.21e-45 159
Msa0341110 AT3G07840.1 37.109 256 138 4 24 259 99 351 3.95e-45 157
Msa0341110 AT2G33160.1 33.775 302 176 8 3 284 94 391 5.55e-44 159
Msa0341110 AT3G14040.1 34.824 313 172 8 1 293 128 428 6.99e-44 155
Msa0341110 AT1G80170.2 38.693 199 106 1 1 183 113 311 1.14e-41 147
Msa0341110 AT2G43880.2 38.865 229 123 5 5 219 94 319 2.77e-40 143
Msa0341110 AT5G39910.1 36.161 224 137 3 1 222 98 317 3.85e-39 141
Msa0341110 AT5G14650.2 40.206 194 102 3 3 183 113 305 1.47e-36 133
Msa0341110 AT3G62110.1 25.862 290 183 6 33 295 149 433 1.26e-20 92.0
Msa0341110 AT3G62110.2 25.862 290 183 6 33 295 149 433 1.26e-20 92.0
Msa0341110 AT4G33440.1 32.701 211 104 6 26 206 172 374 7.83e-20 89.7
Msa0341110 AT3G42950.1 27.425 299 183 8 15 285 154 446 3.64e-18 84.7
Msa0341110 AT2G23900.1 25.413 303 174 11 26 291 156 443 9.89e-18 83.6
Msa0341110 AT3G48950.1 27.700 213 118 7 20 200 139 347 1.97e-16 79.7
Msa0341110 AT5G41870.1 30.909 220 122 6 15 208 134 349 4.15e-16 78.6
Msa0341110 AT4G23820.1 30.693 202 105 7 27 197 136 333 7.73e-16 77.8
Msa0341110 AT3G61490.4 31.053 190 100 6 30 192 261 446 1.71e-15 77.0
Msa0341110 AT3G61490.5 31.053 190 100 6 30 192 163 348 2.46e-15 76.6
Msa0341110 AT3G61490.2 31.053 190 100 6 30 192 148 333 2.85e-15 76.3
Msa0341110 AT3G61490.3 31.053 190 100 6 30 192 148 333 2.85e-15 76.3
Msa0341110 AT3G61490.1 31.053 190 100 6 30 192 148 333 2.85e-15 76.3
Msa0341110 AT1G19170.1 25.828 302 186 10 15 286 176 469 2.18e-14 73.6
Msa0341110 AT4G23500.1 24.549 277 160 10 18 249 160 432 1.78e-12 67.8

Find 71 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAATCCATGTGTTTATT+TGG 0.080796 2_4:+62500764 None:intergenic
ATATCAATGCCATCAGTATT+AGG 0.225865 2_4:+62499946 None:intergenic
TGCTATCGATTTCAAATAAA+AGG 0.252766 2_4:-62500066 Msa0341110:CDS
TTTGATTAACACAATAAAAT+TGG 0.261059 2_4:+62499201 None:intergenic
AACTCTAACAAGCTGCAAAA+AGG 0.263487 2_4:-62500010 Msa0341110:CDS
ACCAATCCATGTGTTTATTT+GGG 0.310797 2_4:+62500765 None:intergenic
TAAAATTGGTCAATCATAAT+GGG 0.312360 2_4:+62499215 None:intergenic
GGTTAGAGCACCCCACATTT+TGG 0.323169 2_4:+62498961 None:intergenic
GGTCAATCATAATGGGGTTA+TGG 0.323679 2_4:+62499222 None:intergenic
TGCAGCTTGTTAGAGTTATA+TGG 0.325682 2_4:+62500016 None:intergenic
ATAAAATTGGTCAATCATAA+TGG 0.333850 2_4:+62499214 None:intergenic
AAGGAGAAAACCTTCATCTT+AGG 0.335531 2_4:+62498903 None:intergenic
TTCAACTAGCATATATTATA+TGG 0.344021 2_4:+62498798 None:intergenic
AAAACAAAATCGGAATATTC+AGG 0.348846 2_4:-62500790 Msa0341110:CDS
TGTTGTAGCATTGGATCATT+GGG 0.360291 2_4:-62499579 Msa0341110:intron
ACGTTGACTGAAACATTAAC+TGG 0.363004 2_4:-62499507 Msa0341110:CDS
CTTAACTCTTTCATTGCAAC+TGG 0.372034 2_4:-62499898 Msa0341110:intron
TTGAGCAAGCAGTTTAGAAT+TGG 0.374243 2_4:+62498843 None:intergenic
TTGTTGTAGCATTGGATCAT+TGG 0.378542 2_4:-62499580 Msa0341110:intron
GTCAATCATAATGGGGTTAT+GGG 0.383263 2_4:+62499223 None:intergenic
AGGCTCTTCCATGAGCATTA+TGG 0.387155 2_4:+62498877 None:intergenic
TATCAATGCCATCAGTATTA+GGG 0.410347 2_4:+62499947 None:intergenic
CAAGCAGTTTAGAATTGGTT+TGG 0.412319 2_4:+62498848 None:intergenic
AACATCCGTTTAATTGCTCC+TGG 0.419774 2_4:-62499976 Msa0341110:CDS
AGAGTTATATGGCTTCTTGC+TGG 0.423641 2_4:+62500027 None:intergenic
GGTTTGATTATAAGTGGAAA+AGG 0.427621 2_4:-62500727 Msa0341110:CDS
GTGGTCCAGGACATGGTATC+AGG 0.443137 2_4:-62499708 Msa0341110:intron
AGTGGAAAAGGGACTATTGA+TGG 0.444615 2_4:-62500715 Msa0341110:CDS
ATGCACATCCTCTACAATAT+CGG 0.446275 2_4:+62499542 None:intergenic
GTCAGTGATGTAACATATAG+AGG 0.449992 2_4:-62499032 Msa0341110:CDS
TGTTGCACCTAAAACAAAAT+CGG 0.452223 2_4:-62500800 Msa0341110:CDS
GTTTGATTATAAGTGGAAAA+GGG 0.469200 2_4:-62500726 Msa0341110:CDS
TCCTTCTGCCATAATGCTCA+TGG 0.470413 2_4:-62498885 Msa0341110:CDS
GTGAATGGTTTGATTATAAG+TGG 0.472070 2_4:-62500733 Msa0341110:CDS
GATTGCATTGCTATTAGTGC+TGG 0.472202 2_4:-62499763 Msa0341110:CDS
ATTGGTTTCTCATTTGTGAA+TGG 0.475752 2_4:-62500748 Msa0341110:CDS
ATCTGCTGTTCCTAAGATGA+AGG 0.477417 2_4:-62498913 Msa0341110:CDS
ATTTCTGAACAGGGTGGAGG+TGG 0.479630 2_4:-62499290 Msa0341110:intron
TCCCAAATAAACACATGGAT+TGG 0.484532 2_4:-62500766 Msa0341110:CDS
TCTCTCCAGGAGCAATTAAA+CGG 0.494330 2_4:+62499971 None:intergenic
GTAACATATAGAGGGATTAG+TGG 0.503191 2_4:-62499023 Msa0341110:CDS
TGTATTTCTGAACAGGGTGG+AGG 0.533598 2_4:-62499293 Msa0341110:intron
ACTAACCTGATACCATGTCC+TGG 0.541363 2_4:+62499703 None:intergenic
TTTGAACTGCGACCAAAATG+TGG 0.542733 2_4:-62498973 Msa0341110:CDS
AGTAAGAATCAAGACAAAGC+AGG 0.556906 2_4:-62499484 Msa0341110:intron
TCAGTATTAGGGCTCTCTCC+AGG 0.559038 2_4:+62499958 None:intergenic
TGCAACTATTATGATTCCAA+AGG 0.560466 2_4:+62498758 None:intergenic
CATAAACACGATCAAATACA+AGG 0.567940 2_4:+62498940 None:intergenic
ACTGCTATAACATGTGGTCC+AGG 0.572929 2_4:-62499721 Msa0341110:CDS
TAGAATTGGTTTGGTGTGTG+AGG 0.574986 2_4:+62498857 None:intergenic
AGGGTGGAGGTGGATTTGCT+AGG 0.585416 2_4:-62499280 Msa0341110:CDS
GTGGAGGTGGATTTGCTAGG+AGG 0.587173 2_4:-62499277 Msa0341110:CDS
TTGTAGCATTGGATCATTGG+GGG 0.588643 2_4:-62499577 Msa0341110:intron
AAAGGGACTATTGATGGTCG+AGG 0.592324 2_4:-62500709 Msa0341110:CDS
GGAGAGAGCCCTAATACTGA+TGG 0.597997 2_4:-62499955 Msa0341110:CDS
TCCATGAGCATTATGGCAGA+AGG 0.604867 2_4:+62498884 None:intergenic
ATAACATGTGGTCCAGGACA+TGG 0.613113 2_4:-62499715 Msa0341110:CDS
CAGGATCCCAAATAAACACA+TGG 0.616392 2_4:-62500771 Msa0341110:CDS
TCAGTGATGTAACATATAGA+GGG 0.625634 2_4:-62499031 Msa0341110:CDS
AAAATAACTGCTATAACATG+TGG 0.629212 2_4:-62499727 Msa0341110:CDS
TTGAACTGCGACCAAAATGT+GGG 0.634447 2_4:-62498972 Msa0341110:CDS
AAGCACTTGAATGTCTCTTG+AGG 0.634460 2_4:+62499918 None:intergenic
GCTCAAGTAGCTAGCTACCT+CGG 0.636157 2_4:-62498826 Msa0341110:exon
AAAATTGGTCAATCATAATG+GGG 0.641528 2_4:+62499216 None:intergenic
GTTGTAGCATTGGATCATTG+GGG 0.669066 2_4:-62499578 Msa0341110:intron
TGGAGACACCGATATTGTAG+AGG 0.670467 2_4:-62499550 Msa0341110:CDS
TGGAACATCATTAACAGACA+AGG 0.670604 2_4:-62499003 Msa0341110:CDS
TTGATGGTCGAGGCTCTATG+TGG 0.678132 2_4:-62500699 Msa0341110:intron
AAAGACACGGTAATCGTACA+CGG 0.682275 2_4:+62498695 None:intergenic
TATATTATATGGTAGCTCCG+AGG 0.755322 2_4:+62498809 None:intergenic
TGAACTGCGACCAAAATGTG+GGG 0.756518 2_4:-62498971 Msa0341110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAACAACAAAAAAAAAA+TGG - chr2_4:62499316-62499335 Msa0341110:intron 10.0%
!! TACATATAAAAATTTACTTA+CGG + chr2_4:62499768-62499787 None:intergenic 10.0%
!!! TTATATGTATAGTTTTTTTA+AGG - chr2_4:62499779-62499798 Msa0341110:intron 10.0%
!!! AATAAGTATTTTCTTAATTA+AGG + chr2_4:62500805-62500824 None:intergenic 10.0%
!!! AGAAATTTAGTTTATTTTTT+GGG + chr2_4:62501299-62501318 None:intergenic 10.0%
!!! TAGAAATTTAGTTTATTTTT+TGG + chr2_4:62501300-62501319 None:intergenic 10.0%
!! NNNAAAACATATGTTATAAG+AGG - chr2_4:62499201-62499220 Msa0341110:CDS 15.0%
!! AAAAATACTTTGGTAAATTA+AGG + chr2_4:62499264-62499283 None:intergenic 15.0%
!!! TTTACCAAAGTATTTTTAAA+TGG - chr2_4:62499267-62499286 Msa0341110:CDS 15.0%
!! ATATCCATTTAAAAATACTT+TGG + chr2_4:62499274-62499293 None:intergenic 15.0%
!!! TTTTTTTTGTTGTTAAATCA+AGG + chr2_4:62499314-62499333 None:intergenic 15.0%
!! TAAAATAAAATAATCCACAA+AGG - chr2_4:62499495-62499514 Msa0341110:CDS 15.0%
!! ACATATAAAAATTTACTTAC+GGG + chr2_4:62499767-62499786 None:intergenic 15.0%
!!! AATTTTTTTTTTCCTTTTTC+AGG - chr2_4:62500146-62500165 Msa0341110:intron 15.0%
!!! ATTTTTTTTTTCCTTTTTCA+GGG - chr2_4:62500147-62500166 Msa0341110:intron 15.0%
!! ATTGTATCTAATCATATTAT+AGG - chr2_4:62500620-62500639 Msa0341110:intron 15.0%
!!! TTTCACTTATACTATAATTT+TGG - chr2_4:62501034-62501053 Msa0341110:intron 15.0%
!! TTTGATTAACACAATAAAAT+TGG + chr2_4:62501212-62501231 None:intergenic 15.0%
!!! ATTTTATTGTGTTAATCAAA+TGG - chr2_4:62501212-62501231 Msa0341110:intron 15.0%
!! AGTAACATATATAAGAAAAA+TGG + chr2_4:62501275-62501294 None:intergenic 15.0%
!!! GAAATTTAGTTTATTTTTTG+GGG + chr2_4:62501298-62501317 None:intergenic 15.0%
!!! TAAGAGGTCAAATTTTTAAA+TGG - chr2_4:62499217-62499236 Msa0341110:CDS 20.0%
!! CAAGTAGTAAAATTCATTAA+AGG - chr2_4:62499432-62499451 Msa0341110:intron 20.0%
!! AATAACGAAATACTTTACAA+TGG - chr2_4:62499459-62499478 Msa0341110:intron 20.0%
!! TGAAAGGTTAAGTTAAAAAA+AGG + chr2_4:62500065-62500084 None:intergenic 20.0%
!!! TTTAAGTAACATAAGTTGAA+AGG + chr2_4:62500081-62500100 None:intergenic 20.0%
!!! TTTTTTTTTTCCTTTTTCAG+GGG - chr2_4:62500148-62500167 Msa0341110:intron 20.0%
!! AAAATAAATACCAGTTCATA+TGG + chr2_4:62500285-62500304 None:intergenic 20.0%
!!! TATTTATTTTTGTGTGTGTA+TGG - chr2_4:62500295-62500314 Msa0341110:intron 20.0%
!!! ATTTTAATTTGTTGTAGCAT+TGG - chr2_4:62500822-62500841 Msa0341110:intron 20.0%
!! ACCAAAATAACTAACATTAT+TGG - chr2_4:62500962-62500981 Msa0341110:intron 20.0%
!!! ACCAATAATGTTAGTTATTT+TGG + chr2_4:62500966-62500985 None:intergenic 20.0%
!! TAAAATTGGTCAATCATAAT+GGG + chr2_4:62501198-62501217 None:intergenic 20.0%
!! ATAAAATTGGTCAATCATAA+TGG + chr2_4:62501199-62501218 None:intergenic 20.0%
!! TTCAACTAGCATATATTATA+TGG + chr2_4:62501615-62501634 None:intergenic 20.0%
!! TGTTGTCTTTGTTCTTATTT+GGG - chr2_4:62499004-62499023 Msa0341110:CDS 25.0%
!!! TCTTATTTGGGTGTTTTTTA+AGG - chr2_4:62499016-62499035 Msa0341110:CDS 25.0%
!!! CTTATTTGGGTGTTTTTTAA+GGG - chr2_4:62499017-62499036 Msa0341110:CDS 25.0%
!!! TTATTTGGGTGTTTTTTAAG+GGG - chr2_4:62499018-62499037 Msa0341110:CDS 25.0%
! AAAAATGGTATAACAATCCA+TGG - chr2_4:62499331-62499350 Msa0341110:intron 25.0%
! ATGATAACATCAAAGATTGA+TGG + chr2_4:62499357-62499376 None:intergenic 25.0%
!! GAATTTTACTACTTGTCTAA+AGG + chr2_4:62499427-62499446 None:intergenic 25.0%
!!! GAATATTCCGATTTTGTTTT+AGG + chr2_4:62499620-62499639 None:intergenic 25.0%
! AAAACAAAATCGGAATATTC+AGG - chr2_4:62499620-62499639 Msa0341110:intron 25.0%
! GTTTGATTATAAGTGGAAAA+GGG - chr2_4:62499684-62499703 Msa0341110:intron 25.0%
! TATAAAAATTTACTTACGGG+TGG + chr2_4:62499764-62499783 None:intergenic 25.0%
!! ATATGTCCTTTAAGTTTCTA+AGG - chr2_4:62499874-62499893 Msa0341110:intron 25.0%
! AAGTTTCAAATGTTACAGAT+AGG + chr2_4:62499996-62500015 None:intergenic 25.0%
!! CTGTAACATTTGAAACTTAA+AGG - chr2_4:62499998-62500017 Msa0341110:CDS 25.0%
! TGATGCAAGTATTACTATAA+AGG + chr2_4:62500118-62500137 None:intergenic 25.0%
!! TCTATAGTATGTATTTTGCA+AGG + chr2_4:62500222-62500241 None:intergenic 25.0%
!!! TGCTATCGATTTCAAATAAA+AGG - chr2_4:62500344-62500363 Msa0341110:intron 25.0%
! AAAATAACTGCTATAACATG+TGG - chr2_4:62500683-62500702 Msa0341110:intron 25.0%
! GCTAAAGAAATTAATACTCA+TGG + chr2_4:62500997-62501016 None:intergenic 25.0%
!! TTTCTATTGTATTTCTGAAC+AGG - chr2_4:62501110-62501129 Msa0341110:intron 25.0%
!! TTCTATTGTATTTCTGAACA+GGG - chr2_4:62501111-62501130 Msa0341110:intron 25.0%
! AAAATTGGTCAATCATAATG+GGG + chr2_4:62501197-62501216 None:intergenic 25.0%
!!! ATCTTTTTTTCTTCTTCCTT+TGG - chr2_4:62501636-62501655 Msa0341110:intron 25.0%
TATAGTTCCGCATCATTATT+TGG - chr2_4:62498979-62498998 Msa0341110:CDS 30.0%
GTGTTGTCTTTGTTCTTATT+TGG - chr2_4:62499003-62499022 Msa0341110:CDS 30.0%
AAAAACATACAAGGGGAAAT+CGG + chr2_4:62499073-62499092 None:intergenic 30.0%
TGGGAAAAAAAACATACAAG+GGG + chr2_4:62499080-62499099 None:intergenic 30.0%
GTGGGAAAAAAAACATACAA+GGG + chr2_4:62499081-62499100 None:intergenic 30.0%
TGTTGCACCTAAAACAAAAT+CGG - chr2_4:62499610-62499629 Msa0341110:intron 30.0%
ACCAATCCATGTGTTTATTT+GGG + chr2_4:62499648-62499667 None:intergenic 30.0%
AACCAATCCATGTGTTTATT+TGG + chr2_4:62499649-62499668 None:intergenic 30.0%
! ATTGGTTTCTCATTTGTGAA+TGG - chr2_4:62499662-62499681 Msa0341110:intron 30.0%
GTGAATGGTTTGATTATAAG+TGG - chr2_4:62499677-62499696 Msa0341110:intron 30.0%
GGTTTGATTATAAGTGGAAA+AGG - chr2_4:62499683-62499702 Msa0341110:intron 30.0%
GTTACAAACGTGAAACTTAA+AGG + chr2_4:62499823-62499842 None:intergenic 30.0%
! ATAGGTCCTTTAAGTTTCAA+AGG - chr2_4:62499845-62499864 Msa0341110:intron 30.0%
!! CTGTAACCTTTGAAACTTAA+AGG + chr2_4:62499854-62499873 None:intergenic 30.0%
CTGAAACCTTAGAAACTTAA+AGG + chr2_4:62499970-62499989 None:intergenic 30.0%
!! AAGTTTCTAAGGTTTCAGAT+AGG - chr2_4:62499885-62499904 Msa0341110:intron 30.0%
GAAACAAACTCGAAACTTAA+AGG + chr2_4:62499912-62499931 None:intergenic 30.0%
!! AAGTTTCTAAGGTTTCAGAT+AGG + chr2_4:62499885-62499904 None:intergenic 30.0%
! GATAGGTCTTTAAGTTTCTA+AGG + chr2_4:62499979-62499998 None:intergenic 30.0%
GTTACAAACGTGAAAGTTAA+AGG - chr2_4:62500029-62500048 Msa0341110:CDS 30.0%
TGAAAGTTAAAGGACGTATT+TGG - chr2_4:62500039-62500058 Msa0341110:CDS 30.0%
TATCAATGCCATCAGTATTA+GGG + chr2_4:62500466-62500485 None:intergenic 30.0%
ATATCAATGCCATCAGTATT+AGG + chr2_4:62500467-62500486 None:intergenic 30.0%
TCTTTAGCACTACTACTATA+AGG - chr2_4:62501009-62501028 Msa0341110:intron 30.0%
!! TAATTTTGGTCACATGACTT+AGG - chr2_4:62501048-62501067 Msa0341110:intron 30.0%
TCAGTGATGTAACATATAGA+GGG - chr2_4:62501379-62501398 Msa0341110:intron 30.0%
CATAAACACGATCAAATACA+AGG + chr2_4:62501473-62501492 None:intergenic 30.0%
TGCAACTATTATGATTCCAA+AGG + chr2_4:62501655-62501674 None:intergenic 30.0%
CAACACACCAAATAATGATG+CGG + chr2_4:62498989-62499008 None:intergenic 35.0%
GGTGGGAAAAAAAACATACA+AGG + chr2_4:62499082-62499101 None:intergenic 35.0%
! GGTAAATTAAGGTATCTTCG+AGG + chr2_4:62499253-62499272 None:intergenic 35.0%
! TTTTGTGAACTCAGCTTTCA+GGG - chr2_4:62499582-62499601 Msa0341110:intron 35.0%
TCCCAAATAAACACATGGAT+TGG - chr2_4:62499644-62499663 Msa0341110:intron 35.0%
AAAAATTTACTTACGGGTGG+TGG + chr2_4:62499761-62499780 None:intergenic 35.0%
! TTTAAGGCCAAATACATCTC+TGG - chr2_4:62499795-62499814 Msa0341110:intron 35.0%
TTAAGGCCAAATACATCTCT+GGG - chr2_4:62499796-62499815 Msa0341110:intron 35.0%
GTTTCACGTTTGTAACAGAT+AGG - chr2_4:62499827-62499846 Msa0341110:intron 35.0%
!! GTTTCGAGTTTGTTTCAGAT+AGG - chr2_4:62499916-62499935 Msa0341110:CDS 35.0%
CTTTCACGTTTGTAACAGAT+AGG + chr2_4:62500025-62500044 None:intergenic 35.0%
TTGTGTGTGTATGGATGTAT+AGG - chr2_4:62500304-62500323 Msa0341110:intron 35.0%
!! TGCAGCTTGTTAGAGTTATA+TGG + chr2_4:62500397-62500416 None:intergenic 35.0%
AACTCTAACAAGCTGCAAAA+AGG - chr2_4:62500400-62500419 Msa0341110:intron 35.0%
ACTCTAACAAGCTGCAAAAA+GGG - chr2_4:62500401-62500420 Msa0341110:intron 35.0%
CTTAACTCTTTCATTGCAAC+TGG - chr2_4:62500512-62500531 Msa0341110:intron 35.0%
! TTGTTGTAGCATTGGATCAT+TGG - chr2_4:62500830-62500849 Msa0341110:intron 35.0%
! TGTTGTAGCATTGGATCATT+GGG - chr2_4:62500831-62500850 Msa0341110:intron 35.0%
ATGCACATCCTCTACAATAT+CGG + chr2_4:62500871-62500890 None:intergenic 35.0%
! ACGTTGACTGAAACATTAAC+TGG - chr2_4:62500903-62500922 Msa0341110:intron 35.0%
AGTAAGAATCAAGACAAAGC+AGG - chr2_4:62500926-62500945 Msa0341110:intron 35.0%
! TATTGTATTTCTGAACAGGG+TGG - chr2_4:62501114-62501133 Msa0341110:intron 35.0%
! GTCAATCATAATGGGGTTAT+GGG + chr2_4:62501190-62501209 None:intergenic 35.0%
TCAAATGGTCTGCAAAAACA+TGG - chr2_4:62501227-62501246 Msa0341110:intron 35.0%
GTCAGTGATGTAACATATAG+AGG - chr2_4:62501378-62501397 Msa0341110:intron 35.0%
GTAACATATAGAGGGATTAG+TGG - chr2_4:62501387-62501406 Msa0341110:intron 35.0%
TGGAACATCATTAACAGACA+AGG - chr2_4:62501407-62501426 Msa0341110:intron 35.0%
AAGGAGAAAACCTTCATCTT+AGG + chr2_4:62501510-62501529 None:intergenic 35.0%
! CAAGCAGTTTAGAATTGGTT+TGG + chr2_4:62501565-62501584 None:intergenic 35.0%
! TTGAGCAAGCAGTTTAGAAT+TGG + chr2_4:62501570-62501589 None:intergenic 35.0%
TATATTATATGGTAGCTCCG+AGG + chr2_4:62501604-62501623 None:intergenic 35.0%
ATGAAGCTGTGGTATTCATG+TGG - chr2_4:62498944-62498963 Msa0341110:CDS 40.0%
! TTGTTCGAGCCTTTTACTTG+TGG - chr2_4:62499044-62499063 Msa0341110:CDS 40.0%
AATCGGTTGCCACAAGTAAA+AGG + chr2_4:62499056-62499075 None:intergenic 40.0%
! ACATCAAAGATTGATGGCCA+TGG + chr2_4:62499351-62499370 None:intergenic 40.0%
! CTTTTGTGAACTCAGCTTTC+AGG - chr2_4:62499581-62499600 Msa0341110:intron 40.0%
CAGGATCCCAAATAAACACA+TGG - chr2_4:62499639-62499658 Msa0341110:intron 40.0%
AGTGGAAAAGGGACTATTGA+TGG - chr2_4:62499695-62499714 Msa0341110:intron 40.0%
AAAGGACCCAGAGATGTATT+TGG + chr2_4:62499805-62499824 None:intergenic 40.0%
!! CTTAACGGAGGTTGATAGAA+AGG + chr2_4:62499943-62499962 None:intergenic 40.0%
CTTTCTATCAACCTCCGTTA+AGG - chr2_4:62499941-62499960 Msa0341110:CDS 40.0%
GGTTTCAGATAGGTCCTTAA+CGG + chr2_4:62499958-62499977 None:intergenic 40.0%
! TACTACTTACTGTTGGTGGA+CGG + chr2_4:62500182-62500201 None:intergenic 40.0%
TAGGTACTACTTACTGTTGG+TGG + chr2_4:62500186-62500205 None:intergenic 40.0%
GAGTAGGTACTACTTACTGT+TGG + chr2_4:62500189-62500208 None:intergenic 40.0%
GCAAGGATCAATACAAGAGT+AGG + chr2_4:62500205-62500224 None:intergenic 40.0%
CTGTTACGTACCATATGAAC+TGG - chr2_4:62500272-62500291 Msa0341110:intron 40.0%
AGAGTTATATGGCTTCTTGC+TGG + chr2_4:62500386-62500405 None:intergenic 40.0%
AACATCCGTTTAATTGCTCC+TGG - chr2_4:62500434-62500453 Msa0341110:intron 40.0%
TCTCTCCAGGAGCAATTAAA+CGG + chr2_4:62500442-62500461 None:intergenic 40.0%
AAGCACTTGAATGTCTCTTG+AGG + chr2_4:62500495-62500514 None:intergenic 40.0%
! GATTGCATTGCTATTAGTGC+TGG - chr2_4:62500647-62500666 Msa0341110:intron 40.0%
! GTTGTAGCATTGGATCATTG+GGG - chr2_4:62500832-62500851 Msa0341110:intron 40.0%
! TTGTAGCATTGGATCATTGG+GGG - chr2_4:62500833-62500852 Msa0341110:intron 40.0%
! GGTCAATCATAATGGGGTTA+TGG + chr2_4:62501191-62501210 None:intergenic 40.0%
TTTGAACTGCGACCAAAATG+TGG - chr2_4:62501437-62501456 Msa0341110:intron 40.0%
TTGAACTGCGACCAAAATGT+GGG - chr2_4:62501438-62501457 Msa0341110:intron 40.0%
ATCTGCTGTTCCTAAGATGA+AGG - chr2_4:62501497-62501516 Msa0341110:intron 40.0%
!! TAGAATTGGTTTGGTGTGTG+AGG + chr2_4:62501556-62501575 None:intergenic 40.0%
GACAACTGATGCAGAAACCA+AGG + chr2_4:62498739-62498758 None:intergenic 45.0%
TCTGCATCAGTTGTCAGATG+CGG - chr2_4:62498744-62498763 Msa0341110:three_prime_UTR 45.0%
!! GCTTGGTTTTTGTGTTGCCT+AGG - chr2_4:62498919-62498938 Msa0341110:CDS 45.0%
ATACCACAGCTTCATCACCT+AGG + chr2_4:62498939-62498958 None:intergenic 45.0%
CTAGAGACTGTTCTCCTTTG+TGG + chr2_4:62499512-62499531 None:intergenic 45.0%
! AAAGGGACTATTGATGGTCG+AGG - chr2_4:62499701-62499720 Msa0341110:intron 45.0%
TGGTGGAAACAACCTTGCAT+AGG - chr2_4:62499731-62499750 Msa0341110:CDS 45.0%
TTCAGATAGGTCCTTAACGG+AGG + chr2_4:62499955-62499974 None:intergenic 45.0%
ACTGCTATAACATGTGGTCC+AGG - chr2_4:62500689-62500708 Msa0341110:intron 45.0%
ATAACATGTGGTCCAGGACA+TGG - chr2_4:62500695-62500714 Msa0341110:intron 45.0%
ACTAACCTGATACCATGTCC+TGG + chr2_4:62500710-62500729 None:intergenic 45.0%
TGGAGACACCGATATTGTAG+AGG - chr2_4:62500860-62500879 Msa0341110:intron 45.0%
! TGTATTTCTGAACAGGGTGG+AGG - chr2_4:62501117-62501136 Msa0341110:intron 45.0%
TGAACTGCGACCAAAATGTG+GGG - chr2_4:62501439-62501458 Msa0341110:intron 45.0%
TCCTTCTGCCATAATGCTCA+TGG - chr2_4:62501525-62501544 Msa0341110:intron 45.0%
TCCATGAGCATTATGGCAGA+AGG + chr2_4:62501529-62501548 None:intergenic 45.0%
AGGCTCTTCCATGAGCATTA+TGG + chr2_4:62501536-62501555 None:intergenic 45.0%
TTTAGCAGCTGTTGCTGCCT+TGG - chr2_4:62498719-62498738 Msa0341110:three_prime_UTR 50.0%
! AGTGCTTGGCTGTTGCTGTT+CGG - chr2_4:62498818-62498837 Msa0341110:three_prime_UTR 50.0%
! GTGTTTGTTGCTGGTGTGGT+TGG - chr2_4:62498846-62498865 Msa0341110:CDS 50.0%
TTGTTGCTGGTGTGGTTGGT+TGG - chr2_4:62498850-62498869 Msa0341110:CDS 50.0%
! TGTTGCTGGTGTGGTTGGTT+GGG - chr2_4:62498851-62498870 Msa0341110:CDS 50.0%
!! GCGTTTCTGGTGTGGTCTTT+GGG - chr2_4:62498875-62498894 Msa0341110:CDS 50.0%
!! CGTTTCTGGTGTGGTCTTTG+GGG - chr2_4:62498876-62498895 Msa0341110:CDS 50.0%
TTGCCTAGGTGATGAAGCTG+TGG - chr2_4:62498933-62498952 Msa0341110:CDS 50.0%
! TTGATGGTCGAGGCTCTATG+TGG - chr2_4:62499711-62499730 Msa0341110:CDS 50.0%
!! GGTGGTGGATTTCCTATGCA+AGG + chr2_4:62499746-62499765 None:intergenic 50.0%
GGCAACTTGTCCCCTGAAAA+AGG + chr2_4:62500161-62500180 None:intergenic 50.0%
TCAGTATTAGGGCTCTCTCC+AGG + chr2_4:62500455-62500474 None:intergenic 50.0%
GGAGAGAGCCCTAATACTGA+TGG - chr2_4:62500455-62500474 Msa0341110:intron 50.0%
ATTTCTGAACAGGGTGGAGG+TGG - chr2_4:62501120-62501139 Msa0341110:intron 50.0%
!! GGTTAGAGCACCCCACATTT+TGG + chr2_4:62501452-62501471 None:intergenic 50.0%
GCTCAAGTAGCTAGCTACCT+CGG - chr2_4:62501584-62501603 Msa0341110:intron 50.0%
GCATCAGTTGTCAGATGCGG+TGG - chr2_4:62498747-62498766 Msa0341110:three_prime_UTR 55.0%
TCAGTTGTCAGATGCGGTGG+TGG - chr2_4:62498750-62498769 Msa0341110:three_prime_UTR 55.0%
! TGTCAGATGCGGTGGTGGTT+AGG - chr2_4:62498755-62498774 Msa0341110:three_prime_UTR 55.0%
TGGTGGTTAGGCAGTGCAGT+TGG - chr2_4:62498767-62498786 Msa0341110:three_prime_UTR 55.0%
GGTGGTTAGGCAGTGCAGTT+GGG - chr2_4:62498768-62498787 Msa0341110:three_prime_UTR 55.0%
CAGCCTGCTACTGAAACGGA+AGG + chr2_4:62498794-62498813 None:intergenic 55.0%
CTTCCGTTTCAGTAGCAGGC+TGG - chr2_4:62498792-62498811 Msa0341110:three_prime_UTR 55.0%
GCACCAGCCTGCTACTGAAA+CGG + chr2_4:62498798-62498817 None:intergenic 55.0%
! TGCGGTGTTTGTTGCTGGTG+TGG - chr2_4:62498842-62498861 Msa0341110:CDS 55.0%
!! CGCGTTTCTGGTGTGGTCTT+TGG - chr2_4:62498874-62498893 Msa0341110:CDS 55.0%
!! TCTGGTGTGGTCTTTGGGGT+AGG - chr2_4:62498880-62498899 Msa0341110:CDS 55.0%
!! CTGGTGTGGTCTTTGGGGTA+GGG - chr2_4:62498881-62498900 Msa0341110:CDS 55.0%
!! TGGTGTGGTCTTTGGGGTAG+GGG - chr2_4:62498882-62498901 Msa0341110:CDS 55.0%
! GGGAGCGGTTATTTGCTGCT+TGG - chr2_4:62498902-62498921 Msa0341110:CDS 55.0%
ATGGTCGAGGCTCTATGTGG+TGG - chr2_4:62499714-62499733 Msa0341110:CDS 55.0%
GTGGTCCAGGACATGGTATC+AGG - chr2_4:62500702-62500721 Msa0341110:CDS 55.0%
! ATTGGATCATTGGGGGCACG+TGG - chr2_4:62500840-62500859 Msa0341110:intron 55.0%
! AGGGTGGAGGTGGATTTGCT+AGG - chr2_4:62501130-62501149 Msa0341110:intron 55.0%
!! GTGGAGGTGGATTTGCTAGG+AGG - chr2_4:62501133-62501152 Msa0341110:intron 55.0%
GGGCCTTCCGTTTCAGTAGC+AGG - chr2_4:62498788-62498807 Msa0341110:three_prime_UTR 60.0%
TAGCAGGCTGGTGCAGTGCT+TGG - chr2_4:62498804-62498823 Msa0341110:three_prime_UTR 60.0%
! TGGCTGTTGCTGTTCGGCTG+CGG - chr2_4:62498824-62498843 Msa0341110:exon 60.0%
! TCGGCTGCGGTGTTTGTTGC+TGG - chr2_4:62498837-62498856 Msa0341110:exon 60.0%
! TGGTTGGTTGGGCGCGTTTC+TGG - chr2_4:62498862-62498881 Msa0341110:CDS 60.0%
!! TGGTCTTTGGGGTAGGGGAG+CGG - chr2_4:62498887-62498906 Msa0341110:CDS 60.0%
!! GGTTGGGCGCGTTTCTGGTG+TGG - chr2_4:62498867-62498886 Msa0341110:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 62498703 62501729 62498703 ID=Msa0341110;Name=Msa0341110
chr2_4 mRNA 62498703 62501729 62498703 ID=Msa0341110-mRNA-1;Parent=Msa0341110;Name=Msa0341110-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.57|0.62|0.87|0.71|0.75|8|136|298
chr2_4 exon 62498703 62499069 62498703 ID=Msa0341110-mRNA-1:exon:19939;Parent=Msa0341110-mRNA-1
chr2_4 exon 62499184 62499300 62499184 ID=Msa0341110-mRNA-1:exon:19938;Parent=Msa0341110-mRNA-1
chr2_4 exon 62499485 62499593 62499485 ID=Msa0341110-mRNA-1:exon:19937;Parent=Msa0341110-mRNA-1
chr2_4 exon 62499709 62499790 62499709 ID=Msa0341110-mRNA-1:exon:19936;Parent=Msa0341110-mRNA-1
chr2_4 exon 62499899 62500106 62499899 ID=Msa0341110-mRNA-1:exon:19935;Parent=Msa0341110-mRNA-1
chr2_4 exon 62500258 62500264 62500258 ID=Msa0341110-mRNA-1:exon:19934;Parent=Msa0341110-mRNA-1
chr2_4 exon 62500700 62500836 62500700 ID=Msa0341110-mRNA-1:exon:19933;Parent=Msa0341110-mRNA-1
chr2_4 exon 62501724 62501729 62501724 ID=Msa0341110-mRNA-1:exon:19932;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62501724 62501729 62501724 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62500700 62500836 62500700 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62500258 62500264 62500258 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62499899 62500106 62499899 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62499709 62499790 62499709 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62499485 62499593 62499485 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62499184 62499300 62499184 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 CDS 62498839 62499069 62498839 ID=Msa0341110-mRNA-1:cds;Parent=Msa0341110-mRNA-1
chr2_4 three_prime_UTR 62498703 62498838 62498703 ID=Msa0341110-mRNA-1:three_prime_utr;Parent=Msa0341110-mRNA-1
Gene Sequence

>Msa0341110

ATGGCTCTTTCAGGGAATATTGTTGCACCTAAAACAAAATCGGAATATTCAGGATCCCAAATAAACACATGGATTGGTTTCTCATTTGTGAATGGTTTGATTATAAGTGGAAAAGGGACTATTGATGGTCGAGGCTCTATGTGGGGACAAGCAATAACATTGAATAGATGCTATCGATTTCAAATAAAAGGATATACAAGTGTTAATCCAGCAAGAAGCCATATAACTCTAACAAGCTGCAAAAAGGGCATTATCTCTAACATCCGTTTAATTGCTCCTGGAGAGAGCCCTAATACTGATGGCATTGATATTTCTGCCTCAAGAGACATTCAAGTGCTTAACTCTTTCATTGCAACTGGTGATGATTGCATTGCTATTAGTGCTGGATCATCAGTAATCAAAATAACTGCTATAACATGTGGTCCAGGACATGGTATCAGCATTGGATCATTGGGGGCACGTGGAGACACCGATATTGTAGAGGATGTGCATGTGAAGAATTGTACGTTGACTGAAACATTAACTGGAGTAAGAATCAAGACAAAGCAGGGTGGAGGTGGATTTGCTAGGAGGATCACGTTTGAAAATATTAGATTTGTTCGAGCCCATAACCCCATTATGATTGACCAATTTTATTGTGTTAATCAAATGGTCTGCAAAAACATGACTAAAGCAATCAAAGTCAGTGATGTAACATATAGAGGGATTAGTGGAACATCATTAACAGACAAGGCAATAAATTTGAACTGCGACCAAAATGTGGGGTGCTCTAACCTTGTATTTGATCGTGTTTATGTAAGATCTGCTGTTCCTAAGATGAAGGTTTTCTCCTTCTGCCATAATGCTCATGGAAGAGCCTCACACACCAAACCAATTCTAAACTGCTTGCTCAAGTAG
Protein sequence

>Msa0341110

MALSGNIVAPKTKSEYSGSQINTWIGFSFVNGLIISGKGTIDGRGSMWGQAITLNRCYRFQIKGYTSVNPARSHITLTSCKKGIISNIRLIAPGESPNTDGIDISASRDIQVLNSFIATGDDCIAISAGSSVIKITAITCGPGHGISIGSLGARGDTDIVEDVHVKNCTLTETLTGVRIKTKQGGGGFARRITFENIRFVRAHNPIMIDQFYCVNQMVCKNMTKAIKVSDVTYRGISGTSLTDKAINLNCDQNVGCSNLVFDRVYVRSAVPKMKVFSFCHNAHGRASHTKPILNCLLK*