AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0374130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374130 MtrunA17_Chr3g0124301 100.000 160 0 0 1 160 1 160 4.99e-120 335
Msa0374130 MtrunA17_Chr3g0115561 39.189 148 82 3 11 152 8 153 1.73e-31 110
Msa0374130 MtrunA17_Chr2g0283801 32.704 159 96 7 1 152 1 155 1.20e-22 88.6
Msa0374130 MtrunA17_Chr2g0316151 29.688 128 85 3 24 151 42 164 3.40e-13 63.9
Msa0374130 MtrunA17_Chr2g0316111 29.688 128 85 3 24 151 42 164 3.82e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374130 AT5G10130.1 46.914 162 81 3 1 159 1 160 6.84e-46 147
Msa0374130 AT4G08685.1 40.000 165 87 4 1 159 1 159 2.70e-36 123
Msa0374130 AT1G78040.1 36.095 169 92 5 1 159 1 163 1.66e-34 119
Msa0374130 AT1G78040.3 36.095 169 92 5 1 159 1 163 1.66e-34 119
Msa0374130 AT1G78040.2 36.095 169 92 5 1 159 1 163 1.66e-34 119
Msa0374130 AT1G29140.1 35.669 157 92 3 4 152 6 161 2.57e-26 98.2
Msa0374130 AT5G45880.1 39.024 123 70 3 25 143 35 156 4.40e-23 90.1
Msa0374130 AT4G18596.1 33.117 154 90 4 1 142 1 153 1.86e-22 88.2
Msa0374130 AT4G18596.2 33.117 154 90 4 1 142 47 199 4.72e-22 88.6

Find 45 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGATTGTGTTGCTGTTAA+TGG 0.278593 3_1:-73326945 Msa0374130:CDS
AATAAACACAACCTTTGATA+TGG 0.316996 3_1:+73326876 None:intergenic
GTGTATTACACGATGATATT+GGG 0.373900 3_1:+73326426 None:intergenic
GGTGTATTACACGATGATAT+TGG 0.406710 3_1:+73326425 None:intergenic
CACTGGAACATACAACATAA+TGG 0.429786 3_1:-73326498 Msa0374130:CDS
CGTCCATAGTCTTCCTGTCT+TGG 0.431661 3_1:+73326552 None:intergenic
ACAAGTAATATTTGAGGAGT+TGG 0.437901 3_1:+73326279 None:intergenic
TAAAGGGAGGATAGAGATGA+GGG 0.446926 3_1:+73326916 None:intergenic
AAATGCTATGGGTTACTTCA+AGG 0.456909 3_1:-73326327 Msa0374130:CDS
TAGGTGCAAAGGTGGGAATT+CGG 0.460306 3_1:-73326577 Msa0374130:CDS
TGGGAATTCGGTGCCAAGAC+AGG 0.469322 3_1:-73326565 Msa0374130:CDS
TATTGTGATACTTGCCGTGC+TGG 0.471219 3_1:-73326857 Msa0374130:CDS
GTGCCAAGACAGGAAGACTA+TGG 0.475430 3_1:-73326555 Msa0374130:CDS
TCTTCACTATGCAAATGCTA+TGG 0.481660 3_1:-73326339 Msa0374130:CDS
TAGCATTTGCATAGTGAAGA+TGG 0.484441 3_1:+73326342 None:intergenic
GATCTGTTACGCCCAGGGTC+CGG 0.486304 3_1:+73326404 None:intergenic
AACATAGCTGTGGCAAGTAA+AGG 0.486675 3_1:+73326899 None:intergenic
ACATAGCTGTGGCAAGTAAA+GGG 0.489307 3_1:+73326900 None:intergenic
GGAGGATAGAGATGAGGGAA+AGG 0.493157 3_1:+73326921 None:intergenic
AGGAGTTGGTGGCAATATTG+AGG 0.508425 3_1:+73326293 None:intergenic
AATCAGACAAGTAATATTTG+AGG 0.510401 3_1:+73326273 None:intergenic
TGCTGGATCTGTTACGCCCA+GGG 0.512778 3_1:+73326399 None:intergenic
AGTAATATTTGAGGAGTTGG+TGG 0.513246 3_1:+73326282 None:intergenic
GTAGTGAGTGAGCACAATGC+TGG 0.521929 3_1:+73326382 None:intergenic
GAGGATAGAGATGAGGGAAA+GGG 0.521934 3_1:+73326922 None:intergenic
ATGCTGGATCTGTTACGCCC+AGG 0.535067 3_1:+73326398 None:intergenic
CTTCACTATGCAAATGCTAT+GGG 0.535954 3_1:-73326338 Msa0374130:CDS
TCGTGTAATACACCGGACCC+TGG 0.543647 3_1:-73326416 Msa0374130:CDS
TTGCATAGTGAAGATGGTTG+AGG 0.549905 3_1:+73326348 None:intergenic
TTCTTGATTAGGTGCAAAGG+TGG 0.550705 3_1:-73326585 Msa0374130:intron
CGTGTAATACACCGGACCCT+GGG 0.556675 3_1:-73326415 Msa0374130:CDS
TGGTGGCAATATTGAGGCAA+AGG 0.557338 3_1:+73326299 None:intergenic
CATTATGTTGTATGTTCCAG+TGG 0.561706 3_1:+73326499 None:intergenic
GTAAAGGGAGGATAGAGATG+AGG 0.562442 3_1:+73326915 None:intergenic
CATTGCTAATCATCATGGCA+AGG 0.587013 3_1:-73326964 None:intergenic
CGATTCTTGATTAGGTGCAA+AGG 0.587702 3_1:-73326588 Msa0374130:intron
ACAGCTATGTTCCATATCAA+AGG 0.587960 3_1:-73326887 Msa0374130:CDS
GAAGGAGTGACAGATTCCAC+TGG 0.617675 3_1:-73326515 Msa0374130:CDS
TTGGATGTAAAATGTGGCGT+TGG 0.634933 3_1:+73326826 None:intergenic
CGTTGGTTTCGAATCCAGCA+CGG 0.637233 3_1:+73326843 None:intergenic
TCTTGATTAGGTGCAAAGGT+GGG 0.642740 3_1:-73326584 Msa0374130:intron
AATATCATCGTGTAATACAC+CGG 0.667061 3_1:-73326423 Msa0374130:CDS
TTTACCTTGGATGTAAAATG+TGG 0.673598 3_1:+73326820 None:intergenic
TTTGATATGGAACATAGCTG+TGG 0.697489 3_1:+73326889 None:intergenic
TAGCTGTGGCAAGTAAAGGG+AGG 0.702643 3_1:+73326903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTAAAAAATTTACGTTG+AGG + chr3_1:73326424-73326443 None:intergenic 20.0%
! AATAAACACAACCTTTGATA+TGG + chr3_1:73326339-73326358 None:intergenic 25.0%
! AATCAGACAAGTAATATTTG+AGG + chr3_1:73326942-73326961 None:intergenic 25.0%
TTTACCTTGGATGTAAAATG+TGG + chr3_1:73326395-73326414 None:intergenic 30.0%
TGTAAAATGTACACATGATC+AGG + chr3_1:73326495-73326514 None:intergenic 30.0%
TATTGTGACGATTCTTGATT+AGG - chr3_1:73326616-73326635 Msa0374130:intron 30.0%
! CAGTTTTGCAAATATTGTCA+TGG + chr3_1:73326753-73326772 None:intergenic 30.0%
GTGTATTACACGATGATATT+GGG + chr3_1:73326789-73326808 None:intergenic 30.0%
AATATCATCGTGTAATACAC+CGG - chr3_1:73326789-73326808 Msa0374130:intron 30.0%
ACAAGTAATATTTGAGGAGT+TGG + chr3_1:73326936-73326955 None:intergenic 30.0%
! AAGGATTGTGTTGCTGTTAA+TGG - chr3_1:73326267-73326286 Msa0374130:CDS 35.0%
TTTGATATGGAACATAGCTG+TGG + chr3_1:73326326-73326345 None:intergenic 35.0%
ACAGCTATGTTCCATATCAA+AGG - chr3_1:73326325-73326344 Msa0374130:CDS 35.0%
CATTATGTTGTATGTTCCAG+TGG + chr3_1:73326716-73326735 None:intergenic 35.0%
CACTGGAACATACAACATAA+TGG - chr3_1:73326714-73326733 Msa0374130:intron 35.0%
GGTGTATTACACGATGATAT+TGG + chr3_1:73326790-73326809 None:intergenic 35.0%
TAGCATTTGCATAGTGAAGA+TGG + chr3_1:73326873-73326892 None:intergenic 35.0%
TCTTCACTATGCAAATGCTA+TGG - chr3_1:73326873-73326892 Msa0374130:CDS 35.0%
CTTCACTATGCAAATGCTAT+GGG - chr3_1:73326874-73326893 Msa0374130:CDS 35.0%
! AAATGCTATGGGTTACTTCA+AGG - chr3_1:73326885-73326904 Msa0374130:CDS 35.0%
AGTAATATTTGAGGAGTTGG+TGG + chr3_1:73326933-73326952 None:intergenic 35.0%
TAAAGGGAGGATAGAGATGA+GGG + chr3_1:73326299-73326318 None:intergenic 40.0%
ACATAGCTGTGGCAAGTAAA+GGG + chr3_1:73326315-73326334 None:intergenic 40.0%
AACATAGCTGTGGCAAGTAA+AGG + chr3_1:73326316-73326335 None:intergenic 40.0%
TTGGATGTAAAATGTGGCGT+TGG + chr3_1:73326389-73326408 None:intergenic 40.0%
! AACGCCACATTTTACATCCA+AGG - chr3_1:73326388-73326407 Msa0374130:CDS 40.0%
GTTGAGGTGTGTATTTACCT+TGG + chr3_1:73326408-73326427 None:intergenic 40.0%
CGATTCTTGATTAGGTGCAA+AGG - chr3_1:73326624-73326643 Msa0374130:intron 40.0%
TTCTTGATTAGGTGCAAAGG+TGG - chr3_1:73326627-73326646 Msa0374130:intron 40.0%
TCTTGATTAGGTGCAAAGGT+GGG - chr3_1:73326628-73326647 Msa0374130:intron 40.0%
! GACGAAGTTTTCTACACAGA+AGG - chr3_1:73326679-73326698 Msa0374130:intron 40.0%
GTGCTCACTCACTACAAAAA+TGG - chr3_1:73326838-73326857 Msa0374130:CDS 40.0%
TTGCATAGTGAAGATGGTTG+AGG + chr3_1:73326867-73326886 None:intergenic 40.0%
GAGGATAGAGATGAGGGAAA+GGG + chr3_1:73326293-73326312 None:intergenic 45.0%
GTAAAGGGAGGATAGAGATG+AGG + chr3_1:73326300-73326319 None:intergenic 45.0%
TATTGTGATACTTGCCGTGC+TGG - chr3_1:73326355-73326374 Msa0374130:CDS 45.0%
TAGGTGCAAAGGTGGGAATT+CGG - chr3_1:73326635-73326654 Msa0374130:intron 45.0%
TGGTGGCAATATTGAGGCAA+AGG + chr3_1:73326916-73326935 None:intergenic 45.0%
!! AGGAGTTGGTGGCAATATTG+AGG + chr3_1:73326922-73326941 None:intergenic 45.0%
GGAGGATAGAGATGAGGGAA+AGG + chr3_1:73326294-73326313 None:intergenic 50.0%
TAGCTGTGGCAAGTAAAGGG+AGG + chr3_1:73326312-73326331 None:intergenic 50.0%
!! CGTTGGTTTCGAATCCAGCA+CGG + chr3_1:73326372-73326391 None:intergenic 50.0%
GTGCCAAGACAGGAAGACTA+TGG - chr3_1:73326657-73326676 Msa0374130:intron 50.0%
CGTCCATAGTCTTCCTGTCT+TGG + chr3_1:73326663-73326682 None:intergenic 50.0%
GAAGGAGTGACAGATTCCAC+TGG - chr3_1:73326697-73326716 Msa0374130:intron 50.0%
GTAGTGAGTGAGCACAATGC+TGG + chr3_1:73326833-73326852 None:intergenic 50.0%
!! TGGGAATTCGGTGCCAAGAC+AGG - chr3_1:73326647-73326666 Msa0374130:intron 55.0%
TCGTGTAATACACCGGACCC+TGG - chr3_1:73326796-73326815 Msa0374130:intron 55.0%
CGTGTAATACACCGGACCCT+GGG - chr3_1:73326797-73326816 Msa0374130:intron 55.0%
TGCTGGATCTGTTACGCCCA+GGG + chr3_1:73326816-73326835 None:intergenic 55.0%
ATGCTGGATCTGTTACGCCC+AGG + chr3_1:73326817-73326836 None:intergenic 55.0%
GATCTGTTACGCCCAGGGTC+CGG + chr3_1:73326811-73326830 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 73326262 73326972 73326262 ID=Msa0374130;Name=Msa0374130
chr3_1 mRNA 73326262 73326972 73326262 ID=Msa0374130-mRNA-1;Parent=Msa0374130;Name=Msa0374130-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|160
chr3_1 exon 73326825 73326972 73326825 ID=Msa0374130-mRNA-1:exon:16677;Parent=Msa0374130-mRNA-1
chr3_1 exon 73326262 73326596 73326262 ID=Msa0374130-mRNA-1:exon:16676;Parent=Msa0374130-mRNA-1
chr3_1 CDS 73326825 73326972 73326825 ID=Msa0374130-mRNA-1:cds;Parent=Msa0374130-mRNA-1
chr3_1 CDS 73326262 73326596 73326262 ID=Msa0374130-mRNA-1:cds;Parent=Msa0374130-mRNA-1
Gene Sequence

>Msa0374130

ATGGCAAGGATTGTGTTGCTGTTAATGGCCCTTTCCCTCATCTCTATCCTCCCTTTACTTGCCACAGCTATGTTCCATATCAAAGGTTGTGTTTATTGTGATACTTGCCGTGCTGGATTCGAAACCAACGCCACATTTTACATCCAAGGTGCAAAGGTGGGAATTCGGTGCCAAGACAGGAAGACTATGGACGAAGTTTTCTACACAGAAGGAGTGACAGATTCCACTGGAACATACAACATAATGGTTGAGAATGATCACCATGACAATATTTGCAAAACTGTGCTTGTTAGCAGCCCAATATCATCGTGTAATACACCGGACCCTGGGCGTAACAGATCCAGCATTGTGCTCACTCACTACAAAAATGGTGTCCTCAACCATCTTCACTATGCAAATGCTATGGGTTACTTCAAGGATGATCCTTTGCCTCAATATTGCCACCAACTCCTCAAATATTACTTGTCTGATTCAGATGTTTGA

Protein sequence

>Msa0374130

MARIVLLLMALSLISILPLLATAMFHIKGCVYCDTCRAGFETNATFYIQGAKVGIRCQDRKTMDEVFYTEGVTDSTGTYNIMVENDHHDNICKTVLVSSPISSCNTPDPGRNRSSIVLTHYKNGVLNHLHYANAMGYFKDDPLPQYCHQLLKYYLSDSDV*