AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0340650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0340650 MtrunA17_Chr2g0329641 95.536 336 12 1 1 333 1 336 0.0 676
Msa0340650 MtrunA17_Chr7g0254231 44.702 302 153 4 33 332 22 311 4.49e-81 249
Msa0340650 MtrunA17_Chr7g0254201 40.924 303 168 4 33 333 20 313 2.93e-76 236
Msa0340650 MtrunA17_Chr7g0258811 39.286 336 182 8 5 332 50 371 1.86e-75 236
Msa0340650 MtrunA17_Chr1g0175561 41.748 309 167 7 32 332 23 326 1.61e-74 233
Msa0340650 MtrunA17_Chr5g0405991 39.458 332 180 8 4 332 3 316 7.40e-71 223
Msa0340650 MtrunA17_Chr2g0299691 38.182 330 191 5 12 333 35 359 2.04e-69 220
Msa0340650 MtrunA17_Chr2g0292241 37.427 342 192 7 1 332 1 330 2.07e-69 220
Msa0340650 MtrunA17_Chr8g0371381 38.614 303 174 6 33 332 26 319 2.08e-69 219
Msa0340650 MtrunA17_Chr2g0292211 37.870 338 190 8 3 332 16 341 2.89e-69 220
Msa0340650 MtrunA17_Chr6g0460711 40.264 303 171 4 32 332 20 314 1.26e-68 217
Msa0340650 MtrunA17_Chr2g0322861 37.771 323 191 5 15 333 71 387 7.54e-68 217
Msa0340650 MtrunA17_Chr4g0063671 39.871 311 175 5 28 332 16 320 2.49e-67 214
Msa0340650 MtrunA17_Chr2g0279041 37.798 336 191 6 2 332 3 325 2.73e-67 214
Msa0340650 MtrunA17_Chr4g0070951 37.942 311 181 5 30 333 25 330 6.15e-67 213
Msa0340650 MtrunA17_Chr4g0024121 38.390 323 189 4 15 332 9 326 3.69e-66 211
Msa0340650 MtrunA17_Chr2g0316831 38.182 330 169 7 32 328 111 438 5.81e-66 214
Msa0340650 MtrunA17_Chr7g0268501 36.228 334 194 6 3 332 2 320 5.92e-66 210
Msa0340650 MtrunA17_Chr7g0268521 36.228 334 194 6 3 332 2 320 5.92e-66 210
Msa0340650 MtrunA17_Chr3g0136751 39.264 326 182 6 11 332 9 322 1.32e-65 209
Msa0340650 MtrunA17_Chr2g0316481 37.805 328 173 6 32 328 75 402 2.73e-65 211
Msa0340650 MtrunA17_Chr2g0292251 37.500 312 177 7 32 333 27 330 3.20e-65 209
Msa0340650 MtrunA17_Chr1g0204011 36.890 328 188 7 9 332 10 322 5.23e-65 208
Msa0340650 MtrunA17_Chr5g0400331 39.610 308 170 6 36 332 29 331 1.06e-64 207
Msa0340650 MtrunA17_Chr2g0316791 37.805 328 173 6 32 328 75 402 1.67e-64 209
Msa0340650 MtrunA17_Chr4g0076211 40.468 299 167 6 37 333 45 334 2.58e-64 206
Msa0340650 MtrunA17_Chr5g0402571 39.542 306 175 5 32 332 26 326 4.16e-64 206
Msa0340650 MtrunA17_Chr2g0292261 35.976 328 198 6 12 332 8 330 5.19e-64 206
Msa0340650 MtrunA17_Chr8g0372601 37.126 334 177 9 14 332 15 330 5.60e-64 206
Msa0340650 MtrunA17_Chr2g0291751 36.036 333 188 8 11 333 14 331 6.34e-64 206
Msa0340650 MtrunA17_Chr5g0402581 39.869 306 174 5 32 332 26 326 1.34e-63 204
Msa0340650 MtrunA17_Chr3g0128061 37.015 335 193 6 3 332 5 326 1.71e-63 204
Msa0340650 MtrunA17_Chr1g0194151 37.879 330 193 7 11 332 8 333 1.98e-63 204
Msa0340650 MtrunA17_Chr5g0404721 38.284 303 176 6 36 332 33 330 3.73e-63 203
Msa0340650 MtrunA17_Chr2g0316191 37.082 329 175 6 32 328 75 403 4.69e-63 206
Msa0340650 MtrunA17_Chr3g0129051 37.338 308 174 6 32 332 27 322 6.55e-63 202
Msa0340650 MtrunA17_Chr5g0438021 36.170 329 188 7 11 332 12 325 3.65e-62 201
Msa0340650 MtrunA17_Chr2g0292271 35.691 311 182 6 32 332 28 330 7.42e-62 201
Msa0340650 MtrunA17_Chr2g0292181 35.045 331 197 7 12 332 17 339 1.05e-61 201
Msa0340650 MtrunA17_Chr1g0157051 38.333 300 172 7 33 328 24 314 1.38e-61 199
Msa0340650 MtrunA17_Chr2g0292111 34.545 330 204 6 11 333 7 331 2.44e-61 199
Msa0340650 MtrunA17_Chr7g0224371 37.542 301 170 5 37 332 35 322 3.16e-61 198
Msa0340650 MtrunA17_Chr1g0194271 37.838 333 196 5 11 332 8 340 5.40e-61 198
Msa0340650 MtrunA17_Chr2g0292151 34.412 340 207 6 1 333 1 331 1.06e-60 197
Msa0340650 MtrunA17_Chr7g0225221 34.024 338 199 7 1 332 1 320 5.44e-60 195
Msa0340650 MtrunA17_Chr4g0013171 37.785 307 172 7 33 332 27 321 6.37e-60 195
Msa0340650 MtrunA17_Chr2g0292091 37.143 280 164 5 60 332 51 325 1.80e-59 194
Msa0340650 MtrunA17_Chr2g0278671 40.193 311 170 7 30 332 21 323 2.21e-59 193
Msa0340650 MtrunA17_Chr2g0311251 36.280 328 177 7 32 328 10 336 4.97e-59 193
Msa0340650 MtrunA17_Chr4g0046131 36.336 333 198 7 5 328 15 342 5.02e-59 193
Msa0340650 MtrunA17_Chr3g0114451 35.207 338 193 6 1 332 1 318 6.21e-59 192
Msa0340650 MtrunA17_Chr5g0421411 39.362 282 148 10 65 332 13 285 7.11e-59 191
Msa0340650 MtrunA17_Chr4g0075241 35.417 336 200 6 5 332 1 327 1.01e-58 192
Msa0340650 MtrunA17_Chr1g0164891 37.785 307 179 7 34 332 90 392 1.67e-58 193
Msa0340650 MtrunA17_Chr1g0188431 35.522 335 199 4 1 332 7 327 1.80e-58 191
Msa0340650 MtrunA17_Chr4g0043391 36.310 336 189 9 7 332 4 324 2.62e-58 191
Msa0340650 MtrunA17_Chr3g0129041 36.585 328 185 7 12 332 11 322 2.89e-58 191
Msa0340650 MtrunA17_Chr4g0013201 35.897 312 180 6 29 332 39 338 3.41e-58 191
Msa0340650 MtrunA17_Chr2g0292101 37.857 280 162 4 60 332 52 326 4.07e-58 191
Msa0340650 MtrunA17_Chr1g0197411 34.118 340 208 6 1 333 1 331 4.60e-58 190
Msa0340650 MtrunA17_Chr3g0129061 36.280 328 186 7 12 332 11 322 7.26e-58 189
Msa0340650 MtrunA17_Chr7g0244461 32.934 334 197 7 4 332 1 312 3.16e-57 187
Msa0340650 MtrunA17_Chr4g0013821 34.426 305 187 5 33 332 28 324 5.25e-57 188
Msa0340650 MtrunA17_Chr3g0106921 34.663 326 193 6 13 332 9 320 5.49e-57 187
Msa0340650 MtrunA17_Chr4g0038161 34.431 334 197 7 7 332 1 320 6.59e-57 187
Msa0340650 MtrunA17_Chr4g0038151 34.431 334 198 8 7 332 1 321 8.39e-57 187
Msa0340650 MtrunA17_Chr4g0013181 37.171 304 172 6 36 332 62 353 9.92e-57 187
Msa0340650 MtrunA17_Chr1g0155611 37.134 307 181 5 33 332 46 347 1.01e-56 187
Msa0340650 MtrunA17_Chr2g0320051 34.756 328 192 7 11 332 7 318 1.03e-56 186
Msa0340650 MtrunA17_Chr2g0311241 33.152 368 175 7 32 328 59 426 1.28e-56 189
Msa0340650 MtrunA17_Chr4g0013721 35.667 300 180 5 38 332 105 396 1.87e-56 188
Msa0340650 MtrunA17_Chr1g0148021 33.140 344 202 7 1 332 1 328 2.96e-56 186
Msa0340650 MtrunA17_Chr2g0292291 33.828 337 211 6 5 333 1 333 4.28e-56 186
Msa0340650 MtrunA17_Chr2g0320071 33.033 333 202 5 5 332 1 317 4.85e-56 184
Msa0340650 MtrunA17_Chr5g0432441 36.156 307 181 5 32 332 29 326 5.38e-56 185
Msa0340650 MtrunA17_Chr3g0107881 36.637 333 181 10 11 332 11 324 5.99e-56 184
Msa0340650 MtrunA17_Chr2g0320061 34.146 328 194 7 11 332 7 318 8.41e-56 184
Msa0340650 MtrunA17_Chr2g0320101 34.451 328 192 7 11 332 7 317 1.22e-55 184
Msa0340650 MtrunA17_Chr4g0072091 36.156 307 184 5 32 332 23 323 1.31e-55 184
Msa0340650 MtrunA17_Chr6g0467251 34.940 332 195 6 11 333 10 329 1.37e-55 184
Msa0340650 MtrunA17_Chr5g0401431 33.657 309 184 5 32 328 26 325 1.90e-55 183
Msa0340650 MtrunA17_Chr6g0477071 33.433 335 200 6 1 332 28 342 2.45e-55 183
Msa0340650 MtrunA17_Chr4g0038171 35.484 310 180 7 32 332 23 321 3.99e-55 182
Msa0340650 MtrunA17_Chr4g0068621 36.877 301 177 5 38 332 43 336 5.89e-55 182
Msa0340650 MtrunA17_Chr2g0279061 40.230 261 145 5 77 332 12 266 6.05e-55 180
Msa0340650 MtrunA17_Chr2g0296621 37.624 303 175 6 33 332 50 341 6.16e-55 182
Msa0340650 MtrunA17_Chr2g0320031 35.061 328 195 8 11 332 4 319 8.81e-55 181
Msa0340650 MtrunA17_Chr4g0013191 37.500 296 170 6 42 332 55 340 1.66e-54 181
Msa0340650 MtrunA17_Chr5g0432341 34.743 331 193 9 13 333 5 322 7.47e-54 179
Msa0340650 MtrunA17_Chr2g0320091 33.951 324 195 7 15 332 8 318 1.49e-53 178
Msa0340650 MtrunA17_Chr5g0432351 34.018 341 193 11 3 333 4 322 1.97e-53 178
Msa0340650 MtrunA17_Chr4g0074851 34.385 317 191 5 24 333 23 329 1.18e-52 176
Msa0340650 MtrunA17_Chr6g0467121 32.934 334 204 7 6 332 43 363 1.52e-52 177
Msa0340650 MtrunA17_Chr1g0181481 33.550 307 193 4 30 332 24 323 1.88e-52 176
Msa0340650 MtrunA17_Chr3g0106901 36.601 306 177 6 32 332 30 323 2.53e-52 175
Msa0340650 MtrunA17_Chr1g0213061 32.628 331 208 6 7 332 1 321 2.67e-52 175
Msa0340650 MtrunA17_Chr1g0181511 32.248 307 197 4 30 332 24 323 3.79e-52 175
Msa0340650 MtrunA17_Chr5g0432331 35.256 312 179 9 32 333 55 353 1.00e-51 174
Msa0340650 MtrunA17_Chr1g0181431 32.787 305 194 4 32 332 26 323 5.93e-51 172
Msa0340650 MtrunA17_Chr6g0467161 32.727 330 202 6 11 332 19 336 1.52e-50 171
Msa0340650 MtrunA17_Chr7g0244451 33.231 325 196 6 13 332 8 316 2.27e-50 170
Msa0340650 MtrunA17_Chr4g0053871 33.443 305 185 6 32 328 26 320 7.49e-50 169
Msa0340650 MtrunA17_Chr4g0004591 33.546 313 154 5 33 328 77 352 2.33e-49 168
Msa0340650 MtrunA17_Chr6g0451571 36.364 308 183 7 32 332 33 334 2.93e-49 167
Msa0340650 MtrunA17_Chr5g0413051 32.154 311 200 4 30 332 70 377 2.57e-48 166
Msa0340650 MtrunA17_Chr8g0364291 31.515 330 212 6 11 332 1 324 1.08e-46 160
Msa0340650 MtrunA17_Chr2g0316811 37.339 233 125 3 32 243 49 281 6.60e-42 148
Msa0340650 MtrunA17_Chr5g0432401 33.550 307 142 9 33 329 29 283 6.69e-41 144
Msa0340650 MtrunA17_Chr2g0292281 36.279 215 118 3 122 332 17 216 2.14e-40 142
Msa0340650 MtrunA17_Chr2g0316141 33.688 282 137 7 79 328 11 274 7.75e-40 141
Msa0340650 MtrunA17_Chr2g0316181 33.688 282 137 7 79 328 11 274 7.75e-40 141
Msa0340650 MtrunA17_Chr3g0114461 31.633 196 117 5 141 332 10 192 5.91e-26 102
Msa0340650 MtrunA17_Chr2g0316771 30.601 183 102 2 173 330 2 184 3.69e-23 94.7
Msa0340650 MtrunA17_Chr1g0181501 29.897 194 126 4 141 332 4 189 5.12e-23 94.4
Msa0340650 MtrunA17_Chr3g0114491 44.545 110 58 1 30 136 15 124 2.38e-20 85.9
Msa0340650 MtrunA17_Chr6g0468731 58.462 65 26 1 72 136 22 85 1.72e-18 79.3
Msa0340650 MtrunA17_Chr3g0104101 33.548 155 83 8 188 332 5 149 4.93e-18 79.7
Msa0340650 MtrunA17_Chr7g0254181 39.216 102 61 1 93 194 22 122 8.47e-17 76.6
Msa0340650 MtrunA17_Chr1g0181491 38.053 113 67 2 30 140 9 120 3.11e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0340650 AT5G24070.1 63.988 336 113 3 4 332 1 335 2.43e-158 446
Msa0340650 AT2G43480.1 66.337 303 99 2 32 332 34 335 3.11e-157 444
Msa0340650 AT5G22410.1 41.049 324 178 6 11 331 11 324 4.54e-75 234
Msa0340650 AT5G17820.1 40.594 303 166 3 32 332 23 313 2.32e-74 232
Msa0340650 AT3G03670.1 40.984 305 171 3 32 332 22 321 1.46e-73 230
Msa0340650 AT5G17820.2 40.594 303 166 3 32 332 76 366 8.97e-73 229
Msa0340650 AT5G06730.1 37.764 331 190 6 9 332 13 334 1.38e-71 226
Msa0340650 AT5G06720.1 37.386 329 190 6 11 332 14 333 2.44e-70 222
Msa0340650 AT5G15180.1 39.803 304 173 4 33 332 32 329 3.09e-70 222
Msa0340650 AT2G41480.1 42.671 307 162 6 33 332 29 328 3.05e-69 219
Msa0340650 AT1G34510.1 39.672 305 164 6 32 332 21 309 8.49e-69 218
Msa0340650 AT5G19880.1 39.701 335 169 10 12 332 14 329 8.14e-68 216
Msa0340650 AT5G39580.1 40.123 324 185 6 11 332 3 319 8.59e-68 215
Msa0340650 AT5G51890.1 39.755 327 181 6 11 332 7 322 8.70e-68 215
Msa0340650 AT4G26010.1 39.344 305 167 5 32 333 21 310 1.17e-67 214
Msa0340650 AT5G66390.1 39.144 327 186 7 12 332 14 333 1.41e-67 215
Msa0340650 AT4G11290.1 38.835 309 179 4 30 332 22 326 2.03e-67 214
Msa0340650 AT1G05260.1 38.961 308 180 5 30 333 23 326 2.84e-67 214
Msa0340650 AT4G26010.2 39.344 305 167 5 32 333 49 338 3.92e-67 214
Msa0340650 AT5G19890.1 37.879 330 184 8 11 333 13 328 1.22e-66 213
Msa0340650 AT5G42180.1 40.000 300 169 6 33 329 23 314 1.55e-66 212
Msa0340650 AT5G64120.1 38.838 327 188 6 11 332 9 328 1.38e-65 210
Msa0340650 AT3G32980.1 37.152 323 186 7 20 333 21 335 2.40e-65 210
Msa0340650 AT4G36430.1 36.196 326 193 6 13 332 14 330 3.70e-65 209
Msa0340650 AT1G05240.1 37.255 306 180 4 33 332 26 325 5.58e-65 208
Msa0340650 AT1G05250.1 37.255 306 180 4 33 332 26 325 5.58e-65 208
Msa0340650 AT4G33870.1 35.758 330 178 4 33 328 68 397 6.19e-65 211
Msa0340650 AT3G50990.1 36.556 331 191 7 9 332 23 341 7.29e-65 209
Msa0340650 AT3G01190.1 38.511 309 182 3 26 332 19 321 5.36e-64 206
Msa0340650 AT2G18140.1 35.484 341 207 8 1 333 1 336 8.62e-64 206
Msa0340650 AT2G39040.1 40.777 309 172 7 32 332 45 350 6.96e-63 204
Msa0340650 AT3G21770.1 36.393 305 186 4 28 328 24 324 1.07e-62 202
Msa0340650 AT5G14130.1 35.030 334 192 7 10 332 11 330 1.12e-62 202
Msa0340650 AT1G30870.1 40.656 305 166 6 33 332 51 345 1.50e-62 202
Msa0340650 AT2G18150.1 35.224 335 200 8 11 333 8 337 1.66e-62 202
Msa0340650 AT3G49110.1 36.446 332 193 8 11 333 15 337 3.49e-62 202
Msa0340650 AT5G14130.2 35.030 334 192 7 10 332 52 371 3.82e-62 202
Msa0340650 AT3G49120.1 36.446 332 193 9 11 333 14 336 2.99e-61 199
Msa0340650 AT2G24800.1 37.785 307 178 6 32 332 30 329 5.55e-61 198
Msa0340650 AT1G68850.1 34.211 342 208 8 1 332 1 335 1.08e-60 197
Msa0340650 AT4G31760.1 35.621 306 185 5 32 332 28 326 4.42e-60 196
Msa0340650 AT5G05340.1 34.839 310 183 5 30 332 27 324 2.11e-59 194
Msa0340650 AT1G14550.1 36.334 311 178 6 30 332 23 321 6.61e-59 192
Msa0340650 AT2G38380.1 35.061 328 194 7 16 332 14 333 8.18e-59 193
Msa0340650 AT5G58400.1 36.615 325 189 6 14 332 12 325 1.12e-58 192
Msa0340650 AT5G64110.1 37.377 305 182 3 31 332 32 330 1.75e-58 192
Msa0340650 AT2G22420.1 35.621 306 184 5 33 333 23 320 2.18e-58 191
Msa0340650 AT4G08770.1 39.716 282 156 7 60 332 51 327 2.40e-58 192
Msa0340650 AT2G18980.1 35.077 325 198 6 14 332 6 323 4.51e-58 190
Msa0340650 AT1G49570.1 37.582 306 180 6 32 330 47 348 4.56e-58 191
Msa0340650 AT2G38390.1 35.077 325 198 6 16 332 14 333 1.64e-57 190
Msa0340650 AT4G37530.1 36.066 305 182 5 32 328 26 325 2.33e-57 189
Msa0340650 AT5G64100.1 38.462 299 171 5 38 332 42 331 3.15e-57 188
Msa0340650 AT4G25980.1 35.329 334 195 4 5 332 8 326 3.87e-57 188
Msa0340650 AT1G14540.1 35.583 326 188 8 13 332 6 315 1.20e-56 186
Msa0340650 AT4G21960.1 35.015 337 198 6 4 333 6 328 1.40e-56 187
Msa0340650 AT1G44970.1 34.604 341 206 6 4 332 11 346 2.21e-56 187
Msa0340650 AT1G71695.1 34.286 315 184 9 28 332 38 339 2.39e-56 187
Msa0340650 AT4G08780.1 36.774 310 182 7 32 332 23 327 2.60e-56 186
Msa0340650 AT5G58390.1 35.238 315 184 5 26 332 14 316 3.50e-56 185
Msa0340650 AT2G35380.1 36.202 337 195 9 7 332 8 335 6.96e-56 185
Msa0340650 AT4G30170.1 35.179 307 186 6 32 332 26 325 5.23e-55 182
Msa0340650 AT4G33420.1 36.964 303 174 4 33 332 37 325 5.23e-55 182
Msa0340650 AT4G37520.1 35.738 305 183 5 32 328 26 325 9.21e-55 182
Msa0340650 AT4G33420.2 36.964 303 174 4 33 332 84 372 1.58e-54 182
Msa0340650 AT2G34060.1 36.422 313 184 7 28 329 35 343 7.92e-54 180
Msa0340650 AT4G16270.1 38.562 306 175 7 33 332 50 348 3.19e-53 179
Msa0340650 AT4G37520.2 35.738 305 180 6 32 328 26 322 1.54e-52 176
Msa0340650 AT1G77100.1 35.119 336 194 8 4 332 1 319 1.59e-52 176
Msa0340650 AT3G17070.1 33.775 302 190 4 33 328 38 335 2.43e-52 176
Msa0340650 AT4G17690.1 35.350 314 186 7 27 333 20 323 6.37e-52 174
Msa0340650 AT5G67400.1 33.538 325 203 5 12 328 6 325 2.81e-51 173
Msa0340650 AT3G49960.1 33.770 305 189 5 32 328 26 325 1.30e-50 171
Msa0340650 AT2G37130.1 32.353 306 195 5 32 332 29 327 1.67e-48 166
Msa0340650 AT5G47000.1 33.435 329 203 7 10 332 12 330 1.29e-47 164
Msa0340650 AT2G37130.2 32.450 302 192 5 36 332 2 296 1.34e-47 162
Msa0340650 AT4G37530.2 37.165 261 150 6 32 284 26 280 2.08e-47 161
Msa0340650 AT1G24110.1 34.146 328 190 10 20 332 6 322 4.99e-45 156
Msa0340650 AT3G17070.2 34.231 260 160 5 75 328 18 272 6.58e-43 149
Msa0340650 AT2G35380.2 36.546 249 144 6 93 332 4 247 4.57e-41 144
Msa0340650 AT5G40150.1 31.511 311 198 5 28 332 27 328 2.64e-40 144
Msa0340650 AT3G28200.1 30.097 309 200 5 30 332 18 316 1.81e-39 142
Msa0340650 AT2G24800.2 43.902 164 89 2 32 193 30 192 5.71e-39 138
Msa0340650 AT5G39580.2 40.404 198 112 3 38 230 30 226 9.02e-37 132
Msa0340650 AT4G25980.2 34.934 229 139 3 13 235 55 279 2.33e-35 130
Msa0340650 AT3G42570.1 34.653 101 63 2 98 195 49 149 1.48e-11 62.0

Find 76 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTCCTTTCTTTGTTCT+TGG 0.121919 2_4:-61995587 None:intergenic
TGCTCAATCCATGTTCAAAA+TGG 0.176494 2_4:+61995793 Msa0340650:CDS
ATCGATCAACAATTACTATT+TGG 0.229792 2_4:+61995707 Msa0340650:CDS
TTTGTCCTTTCTTTGTTCTT+GGG 0.255932 2_4:-61995586 None:intergenic
TCAGAGAATCATACTATAAA+AGG 0.256963 2_4:+61995657 Msa0340650:CDS
TCCTTTCTTCCTGTGAAAAC+TGG 0.305455 2_4:-61995238 None:intergenic
AGCTAGGGATGCTGCTAAAA+TGG 0.328938 2_4:+61995077 Msa0340650:CDS
TGCCATTGCAACTTTGGCTT+TGG 0.331079 2_4:-61994369 None:intergenic
TTAGATATGACAACACTCTT+AGG 0.336759 2_4:+61995358 Msa0340650:CDS
TGACAAGATCAAAACTGTTC+TGG 0.348274 2_4:+61995005 Msa0340650:CDS
AAATTCCTCGGTGATCTCTT+TGG 0.357017 2_4:-61995738 None:intergenic
TCAGCGCAGGAGACAATTCC+AGG 0.375749 2_4:-61995039 None:intergenic
GCTCAATCCATGTTCAAAAT+GGG 0.411312 2_4:+61995794 Msa0340650:CDS
CCTGCGCAATGAAGCTGTCC+TGG 0.413855 2_4:+61995682 Msa0340650:CDS
CTCTAGCATACTTTAAATCA+AGG 0.418727 2_4:+61995323 Msa0340650:CDS
TTGTCCTTTCTTTGTTCTTG+GGG 0.428214 2_4:-61995585 None:intergenic
CTGTATAATTACAATGGTAC+TGG 0.433726 2_4:+61995506 Msa0340650:CDS
CGAAAAGTAGCATCCATGCT+TGG 0.456509 2_4:-61995539 None:intergenic
GAAAAGTAGCATCCATGCTT+GGG 0.480736 2_4:-61995538 None:intergenic
TACTGCCAAGTTACTACGCT+TGG 0.481182 2_4:+61994473 Msa0340650:CDS
TAGTGATGCCATTGCAACTT+TGG 0.483722 2_4:-61994375 None:intergenic
GTAATTGTTGATCGATTCCC+AGG 0.485944 2_4:-61995700 None:intergenic
ACTGCAAAAGCTATTATTAG+TGG 0.486300 2_4:-61994312 None:intergenic
GGCACCAAGTGAAGATTCAC+TGG 0.487472 2_4:+61994433 Msa0340650:CDS
ATCACCGAGGAATTTGCTGC+TGG 0.488175 2_4:+61995746 Msa0340650:CDS
TGTCAACAGGCAGGTGCACC+AGG 0.491420 2_4:+61995211 Msa0340650:intron
CAATGTGGCTACAATGTGTT+TGG 0.492341 2_4:-61995475 None:intergenic
GATCCCAGTGAATCTTCACT+TGG 0.511521 2_4:-61994437 None:intergenic
GTAAACCAAGCGTAGTAACT+TGG 0.512999 2_4:-61994478 None:intergenic
CCAGGACAGCTTCATTGCGC+AGG 0.514985 2_4:-61995682 None:intergenic
ATCACCATCCATCTCATGGC+AGG 0.525540 2_4:+61995297 Msa0340650:CDS
GTTCTGGAATCACGATGCCC+TGG 0.534270 2_4:+61995021 Msa0340650:CDS
CTCCAAAGCCAAAGTTGCAA+TGG 0.535232 2_4:+61994367 Msa0340650:CDS
GTATACCTAAACCCAGACTC+TGG 0.544857 2_4:+61995620 Msa0340650:CDS
TGTCCTTTCTTTGTTCTTGG+GGG 0.550196 2_4:-61995584 None:intergenic
ATATACTCCATCTCGCAGCT+AGG 0.550645 2_4:+61995061 Msa0340650:CDS
CAGCATCCCTAGCTGCGAGA+TGG 0.550746 2_4:-61995068 None:intergenic
CTGCGCAATGAAGCTGTCCT+GGG 0.555200 2_4:+61995683 Msa0340650:CDS
ATTGTAGCCACATTGTTGAC+AGG 0.555596 2_4:+61995483 Msa0340650:CDS
CTGCAAAAGCTATTATTAGT+GGG 0.556067 2_4:-61994311 None:intergenic
GTACAAATCCTCCAAGCCCC+CGG 0.559364 2_4:-61994978 None:intergenic
AATGGGTAGACTTATGTATG+TGG 0.565516 2_4:+61994284 None:intergenic
GCAGTGTTGAGCTTGTGCCA+TGG 0.566470 2_4:+61994330 Msa0340650:CDS
AGTGAATCTTCACTTGGTGC+CGG 0.566856 2_4:-61994431 None:intergenic
CCTGTGAAAACTGGATAACC+TGG 0.572521 2_4:-61995229 None:intergenic
TGCCATCACCATCCATCTCA+TGG 0.573493 2_4:+61995293 Msa0340650:CDS
TCTAGCATACTTTAAATCAA+GGG 0.578417 2_4:+61995324 Msa0340650:CDS
TATACTCCATCTCGCAGCTA+GGG 0.579210 2_4:+61995062 Msa0340650:CDS
GAAATCAAGGAGAGGTACGC+CGG 0.584932 2_4:+61995837 Msa0340650:CDS
ATACTTTAAATCAAGGGGAC+TGG 0.589178 2_4:+61995330 Msa0340650:CDS
GAAAGGACAAAGTGACCCAC+TGG 0.589445 2_4:+61995598 Msa0340650:CDS
GCGACGCAGAGGTGTATGTC+CGG 0.597008 2_4:+61994412 Msa0340650:CDS
GCATCAATACTGCTTGATGA+AGG 0.603718 2_4:+61994925 Msa0340650:CDS
ATGAGATGGATGGTGATGGC+AGG 0.611656 2_4:-61995291 None:intergenic
CTAGCATACTTTAAATCAAG+GGG 0.615884 2_4:+61995325 Msa0340650:CDS
AAATCCAGCAGCAAATTCCT+CGG 0.621559 2_4:-61995750 None:intergenic
AACACAGGAGCACACACACT+AGG 0.622191 2_4:+61995452 Msa0340650:intron
TGGAAACTCAGACCCAAGCA+TGG 0.631385 2_4:+61995526 Msa0340650:CDS
CAATGTATGCCGCGACGCAG+AGG 0.638012 2_4:+61994401 Msa0340650:CDS
GAGATGGAGTATATCAGCGC+AGG 0.640347 2_4:-61995052 None:intergenic
TGCCATGAGATGGATGGTGA+TGG 0.640627 2_4:-61995295 None:intergenic
TAGAGATTCCTGCCATGAGA+TGG 0.641062 2_4:-61995305 None:intergenic
GACAGGCTGTATAATTACAA+TGG 0.641447 2_4:+61995500 Msa0340650:CDS
ACATACATTGACAAAACAGT+CGG 0.657112 2_4:-61994502 None:intergenic
AAAAGAGCACCACAAAACCG+GGG 0.661005 2_4:+61994961 Msa0340650:CDS
TTTAACAGGAAATCAAGGAG+AGG 0.672719 2_4:+61995829 Msa0340650:CDS
CAGCGCAGGAGACAATTCCA+GGG 0.677474 2_4:-61995038 None:intergenic
GCACCAAGTGAAGATTCACT+GGG 0.684010 2_4:+61994434 Msa0340650:CDS
GATTCCTGCCATGAGATGGA+TGG 0.684323 2_4:-61995301 None:intergenic
TATTTGTGTATCGACAACTC+CGG 0.685062 2_4:-61995856 None:intergenic
TCTGGGTTTAGGTATACCAG+TGG 0.695699 2_4:-61995614 None:intergenic
CTGGGTTTAGGTATACCAGT+GGG 0.698864 2_4:-61995613 None:intergenic
TATACAGCCTGTCAACAATG+TGG 0.699068 2_4:-61995490 None:intergenic
GGACATACACCTCTGCGTCG+CGG 0.704072 2_4:-61994410 None:intergenic
AAAGAGCACCACAAAACCGG+GGG 0.709202 2_4:+61994962 Msa0340650:CDS
TGACACCAAAGAGATCACCG+AGG 0.853016 2_4:+61995733 Msa0340650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAGAAAAAAGGAAAT+GGG - chr2_4:61994529-61994548 None:intergenic 15.0%
!! TTATAAAAGAAAAAAGGAAA+TGG - chr2_4:61994530-61994549 None:intergenic 15.0%
!!! TCCTTTTTTCTTTTATAATT+TGG + chr2_4:61994532-61994551 Msa0340650:intron 15.0%
!! ACCAAATTATAAAAGAAAAA+AGG - chr2_4:61994536-61994555 None:intergenic 15.0%
!! TTCATTTATAATGTTTAGAA+TGG - chr2_4:61994844-61994863 None:intergenic 15.0%
!! TATAAAAGAAAAAAGGAAAT+GGG - chr2_4:61994529-61994548 None:intergenic 15.0%
!! TTATAAAAGAAAAAAGGAAA+TGG - chr2_4:61994530-61994549 None:intergenic 15.0%
!!! TCCTTTTTTCTTTTATAATT+TGG + chr2_4:61994532-61994551 Msa0340650:intron 15.0%
!! ACCAAATTATAAAAGAAAAA+AGG - chr2_4:61994536-61994555 None:intergenic 15.0%
!! TTCATTTATAATGTTTAGAA+TGG - chr2_4:61994844-61994863 None:intergenic 15.0%
!! ATAAAAGAAAAAAGGAAATG+GGG - chr2_4:61994528-61994547 None:intergenic 20.0%
!! TAGCTTAAAAAGTTGAAAAA+TGG - chr2_4:61994581-61994600 None:intergenic 20.0%
!!! TTTAGAATGGTAAGTTTAAA+TGG - chr2_4:61994831-61994850 None:intergenic 20.0%
!!! TACTAACAATATGTTCTAAA+TGG + chr2_4:61995413-61995432 Msa0340650:intron 20.0%
!!! ACTAACAATATGTTCTAAAT+GGG + chr2_4:61995414-61995433 Msa0340650:intron 20.0%
!! ATAAAAGAAAAAAGGAAATG+GGG - chr2_4:61994528-61994547 None:intergenic 20.0%
!! TAGCTTAAAAAGTTGAAAAA+TGG - chr2_4:61994581-61994600 None:intergenic 20.0%
!!! TTTAGAATGGTAAGTTTAAA+TGG - chr2_4:61994831-61994850 None:intergenic 20.0%
!!! TACTAACAATATGTTCTAAA+TGG + chr2_4:61995413-61995432 Msa0340650:intron 20.0%
!!! ACTAACAATATGTTCTAAAT+GGG + chr2_4:61995414-61995433 Msa0340650:intron 20.0%
!!! TGCATATATTTTAGCTTCAA+GGG - chr2_4:61994696-61994715 None:intergenic 25.0%
!!! TTGCATATATTTTAGCTTCA+AGG - chr2_4:61994697-61994716 None:intergenic 25.0%
! ATTTAGATAGAACAACAAGA+GGG - chr2_4:61994752-61994771 None:intergenic 25.0%
! AATTTAGATAGAACAACAAG+AGG - chr2_4:61994753-61994772 None:intergenic 25.0%
! ACTCTAAATACCAAGTTAAA+TGG - chr2_4:61994784-61994803 None:intergenic 25.0%
! GTTGACATTAATTCTCAAAT+CGG - chr2_4:61995198-61995217 None:intergenic 25.0%
! ATTTGAGAATTAATGTCAAC+AGG + chr2_4:61995198-61995217 Msa0340650:intron 25.0%
! TCTAGCATACTTTAAATCAA+GGG + chr2_4:61995324-61995343 Msa0340650:CDS 25.0%
! TCAGAGAATCATACTATAAA+AGG + chr2_4:61995657-61995676 Msa0340650:CDS 25.0%
! ATCGATCAACAATTACTATT+TGG + chr2_4:61995707-61995726 Msa0340650:CDS 25.0%
!!! GGAAATATCAAAGTTTTAAC+AGG + chr2_4:61995815-61995834 Msa0340650:CDS 25.0%
!!! AAAGTTTTAACAGGAAATCA+AGG + chr2_4:61995824-61995843 Msa0340650:CDS 25.0%
!!! TGCATATATTTTAGCTTCAA+GGG - chr2_4:61994696-61994715 None:intergenic 25.0%
!!! TTGCATATATTTTAGCTTCA+AGG - chr2_4:61994697-61994716 None:intergenic 25.0%
! ATTTAGATAGAACAACAAGA+GGG - chr2_4:61994752-61994771 None:intergenic 25.0%
! AATTTAGATAGAACAACAAG+AGG - chr2_4:61994753-61994772 None:intergenic 25.0%
! ACTCTAAATACCAAGTTAAA+TGG - chr2_4:61994784-61994803 None:intergenic 25.0%
! GTTGACATTAATTCTCAAAT+CGG - chr2_4:61995198-61995217 None:intergenic 25.0%
! ATTTGAGAATTAATGTCAAC+AGG + chr2_4:61995198-61995217 Msa0340650:intron 25.0%
! TCTAGCATACTTTAAATCAA+GGG + chr2_4:61995324-61995343 Msa0340650:CDS 25.0%
! TCAGAGAATCATACTATAAA+AGG + chr2_4:61995657-61995676 Msa0340650:CDS 25.0%
! ATCGATCAACAATTACTATT+TGG + chr2_4:61995707-61995726 Msa0340650:CDS 25.0%
!!! GGAAATATCAAAGTTTTAAC+AGG + chr2_4:61995815-61995834 Msa0340650:CDS 25.0%
!!! AAAGTTTTAACAGGAAATCA+AGG + chr2_4:61995824-61995843 Msa0340650:CDS 25.0%
CTGCAAAAGCTATTATTAGT+GGG - chr2_4:61994314-61994333 None:intergenic 30.0%
ACTGCAAAAGCTATTATTAG+TGG - chr2_4:61994315-61994334 None:intergenic 30.0%
ACATACATTGACAAAACAGT+CGG - chr2_4:61994505-61994524 None:intergenic 30.0%
!! GAAAGGAACTTTTAGGTAAT+AGG + chr2_4:61994631-61994650 Msa0340650:intron 30.0%
!! AACTTTTAGGTAATAGGACT+AGG + chr2_4:61994637-61994656 Msa0340650:intron 30.0%
TTCAGTTGTTCCATTTAACT+TGG + chr2_4:61994771-61994790 Msa0340650:intron 30.0%
AAAAATCTTCTCTCAAGGTA+GGG + chr2_4:61995150-61995169 Msa0340650:intron 30.0%
CTCTAGCATACTTTAAATCA+AGG + chr2_4:61995323-61995342 Msa0340650:CDS 30.0%
CTAGCATACTTTAAATCAAG+GGG + chr2_4:61995325-61995344 Msa0340650:CDS 30.0%
TTAGATATGACAACACTCTT+AGG + chr2_4:61995358-61995377 Msa0340650:CDS 30.0%
CTGTATAATTACAATGGTAC+TGG + chr2_4:61995506-61995525 Msa0340650:CDS 30.0%
TTTGTCCTTTCTTTGTTCTT+GGG - chr2_4:61995589-61995608 None:intergenic 30.0%
! GATATTTCCCATTTTGAACA+TGG - chr2_4:61995804-61995823 None:intergenic 30.0%
CTGCAAAAGCTATTATTAGT+GGG - chr2_4:61994314-61994333 None:intergenic 30.0%
ACTGCAAAAGCTATTATTAG+TGG - chr2_4:61994315-61994334 None:intergenic 30.0%
ACATACATTGACAAAACAGT+CGG - chr2_4:61994505-61994524 None:intergenic 30.0%
!! GAAAGGAACTTTTAGGTAAT+AGG + chr2_4:61994631-61994650 Msa0340650:intron 30.0%
!! AACTTTTAGGTAATAGGACT+AGG + chr2_4:61994637-61994656 Msa0340650:intron 30.0%
TTCAGTTGTTCCATTTAACT+TGG + chr2_4:61994771-61994790 Msa0340650:intron 30.0%
AAAAATCTTCTCTCAAGGTA+GGG + chr2_4:61995150-61995169 Msa0340650:intron 30.0%
CTCTAGCATACTTTAAATCA+AGG + chr2_4:61995323-61995342 Msa0340650:CDS 30.0%
CTAGCATACTTTAAATCAAG+GGG + chr2_4:61995325-61995344 Msa0340650:CDS 30.0%
TTAGATATGACAACACTCTT+AGG + chr2_4:61995358-61995377 Msa0340650:CDS 30.0%
CTGTATAATTACAATGGTAC+TGG + chr2_4:61995506-61995525 Msa0340650:CDS 30.0%
TTTGTCCTTTCTTTGTTCTT+GGG - chr2_4:61995589-61995608 None:intergenic 30.0%
! GATATTTCCCATTTTGAACA+TGG - chr2_4:61995804-61995823 None:intergenic 30.0%
! ATTGGCTGAAAGGAACTTTT+AGG + chr2_4:61994624-61994643 Msa0340650:intron 35.0%
!! TGTTTTTGTAACCTGTCCTT+TGG - chr2_4:61994668-61994687 None:intergenic 35.0%
! CTTGGTATTTAGAGTTCATG+AGG + chr2_4:61994789-61994808 Msa0340650:intron 35.0%
! GTTCATGAGGTTTAAAGCAT+AGG + chr2_4:61994802-61994821 Msa0340650:intron 35.0%
! GAAACAGCGATTTAAGTTTC+AGG - chr2_4:61994887-61994906 None:intergenic 35.0%
! AAACTTAAATCGCTGTTTCG+TGG + chr2_4:61994888-61994907 Msa0340650:intron 35.0%
! TTTTGTGGTGCTCTTTTTTC+TGG - chr2_4:61994958-61994977 None:intergenic 35.0%
!! AAAAAAGAGCACCACAAAAC+CGG + chr2_4:61994959-61994978 Msa0340650:CDS 35.0%
TGACAAGATCAAAACTGTTC+TGG + chr2_4:61995005-61995024 Msa0340650:CDS 35.0%
! CTTGAGAGAAGATTTTTCTG+CGG - chr2_4:61995147-61995166 None:intergenic 35.0%
GAAAAATCTTCTCTCAAGGT+AGG + chr2_4:61995149-61995168 Msa0340650:intron 35.0%
AAAATCTTCTCTCAAGGTAG+GGG + chr2_4:61995151-61995170 Msa0340650:intron 35.0%
ATACTTTAAATCAAGGGGAC+TGG + chr2_4:61995330-61995349 Msa0340650:CDS 35.0%
TATGACAACACTCTTAGGTA+AGG + chr2_4:61995363-61995382 Msa0340650:intron 35.0%
TGCATGTTTCATATCAACAC+AGG + chr2_4:61995437-61995456 Msa0340650:intron 35.0%
GACAGGCTGTATAATTACAA+TGG + chr2_4:61995500-61995519 Msa0340650:CDS 35.0%
TTGTCCTTTCTTTGTTCTTG+GGG - chr2_4:61995588-61995607 None:intergenic 35.0%
CTTTGTCCTTTCTTTGTTCT+TGG - chr2_4:61995590-61995609 None:intergenic 35.0%
! TGTAATCTTTTCCAGAGTCT+GGG - chr2_4:61995634-61995653 None:intergenic 35.0%
! ATGTAATCTTTTCCAGAGTC+TGG - chr2_4:61995635-61995654 None:intergenic 35.0%
! CTGCTGGATTTGAAGATTTT+AGG + chr2_4:61995762-61995781 Msa0340650:CDS 35.0%
TGCTCAATCCATGTTCAAAA+TGG + chr2_4:61995793-61995812 Msa0340650:CDS 35.0%
GCTCAATCCATGTTCAAAAT+GGG + chr2_4:61995794-61995813 Msa0340650:CDS 35.0%
TTTAACAGGAAATCAAGGAG+AGG + chr2_4:61995829-61995848 Msa0340650:CDS 35.0%
! TATTTGTGTATCGACAACTC+CGG - chr2_4:61995859-61995878 None:intergenic 35.0%
! ATTGGCTGAAAGGAACTTTT+AGG + chr2_4:61994624-61994643 Msa0340650:intron 35.0%
!! TGTTTTTGTAACCTGTCCTT+TGG - chr2_4:61994668-61994687 None:intergenic 35.0%
! CTTGGTATTTAGAGTTCATG+AGG + chr2_4:61994789-61994808 Msa0340650:intron 35.0%
! GTTCATGAGGTTTAAAGCAT+AGG + chr2_4:61994802-61994821 Msa0340650:intron 35.0%
! GAAACAGCGATTTAAGTTTC+AGG - chr2_4:61994887-61994906 None:intergenic 35.0%
! AAACTTAAATCGCTGTTTCG+TGG + chr2_4:61994888-61994907 Msa0340650:intron 35.0%
! TTTTGTGGTGCTCTTTTTTC+TGG - chr2_4:61994958-61994977 None:intergenic 35.0%
!! AAAAAAGAGCACCACAAAAC+CGG + chr2_4:61994959-61994978 Msa0340650:CDS 35.0%
TGACAAGATCAAAACTGTTC+TGG + chr2_4:61995005-61995024 Msa0340650:CDS 35.0%
! CTTGAGAGAAGATTTTTCTG+CGG - chr2_4:61995147-61995166 None:intergenic 35.0%
GAAAAATCTTCTCTCAAGGT+AGG + chr2_4:61995149-61995168 Msa0340650:intron 35.0%
AAAATCTTCTCTCAAGGTAG+GGG + chr2_4:61995151-61995170 Msa0340650:intron 35.0%
ATACTTTAAATCAAGGGGAC+TGG + chr2_4:61995330-61995349 Msa0340650:CDS 35.0%
TATGACAACACTCTTAGGTA+AGG + chr2_4:61995363-61995382 Msa0340650:intron 35.0%
TGCATGTTTCATATCAACAC+AGG + chr2_4:61995437-61995456 Msa0340650:intron 35.0%
GACAGGCTGTATAATTACAA+TGG + chr2_4:61995500-61995519 Msa0340650:CDS 35.0%
TTGTCCTTTCTTTGTTCTTG+GGG - chr2_4:61995588-61995607 None:intergenic 35.0%
CTTTGTCCTTTCTTTGTTCT+TGG - chr2_4:61995590-61995609 None:intergenic 35.0%
! TGTAATCTTTTCCAGAGTCT+GGG - chr2_4:61995634-61995653 None:intergenic 35.0%
! ATGTAATCTTTTCCAGAGTC+TGG - chr2_4:61995635-61995654 None:intergenic 35.0%
! CTGCTGGATTTGAAGATTTT+AGG + chr2_4:61995762-61995781 Msa0340650:CDS 35.0%
TGCTCAATCCATGTTCAAAA+TGG + chr2_4:61995793-61995812 Msa0340650:CDS 35.0%
GCTCAATCCATGTTCAAAAT+GGG + chr2_4:61995794-61995813 Msa0340650:CDS 35.0%
TTTAACAGGAAATCAAGGAG+AGG + chr2_4:61995829-61995848 Msa0340650:CDS 35.0%
! TATTTGTGTATCGACAACTC+CGG - chr2_4:61995859-61995878 None:intergenic 35.0%
TAGTGATGCCATTGCAACTT+TGG - chr2_4:61994378-61994397 None:intergenic 40.0%
GTAAACCAAGCGTAGTAACT+TGG - chr2_4:61994481-61994500 None:intergenic 40.0%
TAAGAAAGAGACATCGTGTG+AGG + chr2_4:61994599-61994618 Msa0340650:intron 40.0%
ATAGGACTAGGAACAACCAA+AGG + chr2_4:61994649-61994668 Msa0340650:intron 40.0%
GCATCAATACTGCTTGATGA+AGG + chr2_4:61994925-61994944 Msa0340650:CDS 40.0%
!!! GGTGCTCTTTTTTCTGGATT+TGG - chr2_4:61994952-61994971 None:intergenic 40.0%
!! AAAAAGAGCACCACAAAACC+GGG + chr2_4:61994960-61994979 Msa0340650:CDS 40.0%
! AGAGAAGATTTTTCTGCGGA+GGG - chr2_4:61995143-61995162 None:intergenic 40.0%
CGCAGAAAAATCTTCTCTCA+AGG + chr2_4:61995145-61995164 Msa0340650:intron 40.0%
GAGAATTAATGTCAACAGGC+AGG + chr2_4:61995202-61995221 Msa0340650:intron 40.0%
! TCCAGTTTTCACAGGAAGAA+AGG + chr2_4:61995237-61995256 Msa0340650:CDS 40.0%
TCCTTTCTTCCTGTGAAAAC+TGG - chr2_4:61995241-61995260 None:intergenic 40.0%
! GTTTTCACAGGAAGAAAGGA+TGG + chr2_4:61995241-61995260 Msa0340650:CDS 40.0%
CAATGTGGCTACAATGTGTT+TGG - chr2_4:61995478-61995497 None:intergenic 40.0%
ATTGTAGCCACATTGTTGAC+AGG + chr2_4:61995483-61995502 Msa0340650:CDS 40.0%
TATACAGCCTGTCAACAATG+TGG - chr2_4:61995493-61995512 None:intergenic 40.0%
GAAAAGTAGCATCCATGCTT+GGG - chr2_4:61995541-61995560 None:intergenic 40.0%
!! TACTTTTCGAGACACGTTGA+GGG + chr2_4:61995553-61995572 Msa0340650:CDS 40.0%
TGTCCTTTCTTTGTTCTTGG+GGG - chr2_4:61995587-61995606 None:intergenic 40.0%
! GTAATTGTTGATCGATTCCC+AGG - chr2_4:61995703-61995722 None:intergenic 40.0%
AAATTCCTCGGTGATCTCTT+TGG - chr2_4:61995741-61995760 None:intergenic 40.0%
AAATCCAGCAGCAAATTCCT+CGG - chr2_4:61995753-61995772 None:intergenic 40.0%
TAGTGATGCCATTGCAACTT+TGG - chr2_4:61994378-61994397 None:intergenic 40.0%
GTAAACCAAGCGTAGTAACT+TGG - chr2_4:61994481-61994500 None:intergenic 40.0%
TAAGAAAGAGACATCGTGTG+AGG + chr2_4:61994599-61994618 Msa0340650:intron 40.0%
ATAGGACTAGGAACAACCAA+AGG + chr2_4:61994649-61994668 Msa0340650:intron 40.0%
GCATCAATACTGCTTGATGA+AGG + chr2_4:61994925-61994944 Msa0340650:CDS 40.0%
!!! GGTGCTCTTTTTTCTGGATT+TGG - chr2_4:61994952-61994971 None:intergenic 40.0%
!! AAAAAGAGCACCACAAAACC+GGG + chr2_4:61994960-61994979 Msa0340650:CDS 40.0%
! AGAGAAGATTTTTCTGCGGA+GGG - chr2_4:61995143-61995162 None:intergenic 40.0%
CGCAGAAAAATCTTCTCTCA+AGG + chr2_4:61995145-61995164 Msa0340650:intron 40.0%
GAGAATTAATGTCAACAGGC+AGG + chr2_4:61995202-61995221 Msa0340650:intron 40.0%
! TCCAGTTTTCACAGGAAGAA+AGG + chr2_4:61995237-61995256 Msa0340650:CDS 40.0%
TCCTTTCTTCCTGTGAAAAC+TGG - chr2_4:61995241-61995260 None:intergenic 40.0%
! GTTTTCACAGGAAGAAAGGA+TGG + chr2_4:61995241-61995260 Msa0340650:CDS 40.0%
CAATGTGGCTACAATGTGTT+TGG - chr2_4:61995478-61995497 None:intergenic 40.0%
ATTGTAGCCACATTGTTGAC+AGG + chr2_4:61995483-61995502 Msa0340650:CDS 40.0%
TATACAGCCTGTCAACAATG+TGG - chr2_4:61995493-61995512 None:intergenic 40.0%
GAAAAGTAGCATCCATGCTT+GGG - chr2_4:61995541-61995560 None:intergenic 40.0%
!! TACTTTTCGAGACACGTTGA+GGG + chr2_4:61995553-61995572 Msa0340650:CDS 40.0%
TGTCCTTTCTTTGTTCTTGG+GGG - chr2_4:61995587-61995606 None:intergenic 40.0%
! GTAATTGTTGATCGATTCCC+AGG - chr2_4:61995703-61995722 None:intergenic 40.0%
AAATTCCTCGGTGATCTCTT+TGG - chr2_4:61995741-61995760 None:intergenic 40.0%
AAATCCAGCAGCAAATTCCT+CGG - chr2_4:61995753-61995772 None:intergenic 40.0%
CTCCAAAGCCAAAGTTGCAA+TGG + chr2_4:61994367-61994386 Msa0340650:CDS 45.0%
! TGCCATTGCAACTTTGGCTT+TGG - chr2_4:61994372-61994391 None:intergenic 45.0%
!! AGTGAATCTTCACTTGGTGC+CGG - chr2_4:61994434-61994453 None:intergenic 45.0%
GCACCAAGTGAAGATTCACT+GGG + chr2_4:61994434-61994453 Msa0340650:CDS 45.0%
GATCCCAGTGAATCTTCACT+TGG - chr2_4:61994440-61994459 None:intergenic 45.0%
TACTGCCAAGTTACTACGCT+TGG + chr2_4:61994473-61994492 Msa0340650:CDS 45.0%
GAGACATCGTGTGAGGTTAT+TGG + chr2_4:61994606-61994625 Msa0340650:intron 45.0%
! GTGTGAGGTTATTGGCTGAA+AGG + chr2_4:61994614-61994633 Msa0340650:intron 45.0%
ACTAGGAACAACCAAAGGAC+AGG + chr2_4:61994654-61994673 Msa0340650:intron 45.0%
! AAATCGCTGTTTCGTGGCAT+AGG + chr2_4:61994894-61994913 Msa0340650:intron 45.0%
! AATCGCTGTTTCGTGGCATA+GGG + chr2_4:61994895-61994914 Msa0340650:intron 45.0%
!! AAAAGAGCACCACAAAACCG+GGG + chr2_4:61994961-61994980 Msa0340650:CDS 45.0%
ATATACTCCATCTCGCAGCT+AGG + chr2_4:61995061-61995080 Msa0340650:CDS 45.0%
TATACTCCATCTCGCAGCTA+GGG + chr2_4:61995062-61995081 Msa0340650:CDS 45.0%
AGCTAGGGATGCTGCTAAAA+TGG + chr2_4:61995077-61995096 Msa0340650:CDS 45.0%
! GAGAAGATTTTTCTGCGGAG+GGG - chr2_4:61995142-61995161 None:intergenic 45.0%
! GAGAGAAGATTTTTCTGCGG+AGG - chr2_4:61995144-61995163 None:intergenic 45.0%
CCTGTGAAAACTGGATAACC+TGG - chr2_4:61995232-61995251 None:intergenic 45.0%
! CCAGGTTATCCAGTTTTCAC+AGG + chr2_4:61995229-61995248 Msa0340650:CDS 45.0%
TAGAGATTCCTGCCATGAGA+TGG - chr2_4:61995308-61995327 None:intergenic 45.0%
CGAAAAGTAGCATCCATGCT+TGG - chr2_4:61995542-61995561 None:intergenic 45.0%
!! CTACTTTTCGAGACACGTTG+AGG + chr2_4:61995552-61995571 Msa0340650:CDS 45.0%
!! ACTTTTCGAGACACGTTGAG+GGG + chr2_4:61995554-61995573 Msa0340650:CDS 45.0%
TGTCCCCCAAGAACAAAGAA+AGG + chr2_4:61995581-61995600 Msa0340650:CDS 45.0%
CTGGGTTTAGGTATACCAGT+GGG - chr2_4:61995616-61995635 None:intergenic 45.0%
TCTGGGTTTAGGTATACCAG+TGG - chr2_4:61995617-61995636 None:intergenic 45.0%
GTATACCTAAACCCAGACTC+TGG + chr2_4:61995620-61995639 Msa0340650:CDS 45.0%
! CTTTTCCAGAGTCTGGGTTT+AGG - chr2_4:61995628-61995647 None:intergenic 45.0%
CTCCAAAGCCAAAGTTGCAA+TGG + chr2_4:61994367-61994386 Msa0340650:CDS 45.0%
! TGCCATTGCAACTTTGGCTT+TGG - chr2_4:61994372-61994391 None:intergenic 45.0%
!! AGTGAATCTTCACTTGGTGC+CGG - chr2_4:61994434-61994453 None:intergenic 45.0%
GCACCAAGTGAAGATTCACT+GGG + chr2_4:61994434-61994453 Msa0340650:CDS 45.0%
GATCCCAGTGAATCTTCACT+TGG - chr2_4:61994440-61994459 None:intergenic 45.0%
TACTGCCAAGTTACTACGCT+TGG + chr2_4:61994473-61994492 Msa0340650:CDS 45.0%
GAGACATCGTGTGAGGTTAT+TGG + chr2_4:61994606-61994625 Msa0340650:intron 45.0%
! GTGTGAGGTTATTGGCTGAA+AGG + chr2_4:61994614-61994633 Msa0340650:intron 45.0%
ACTAGGAACAACCAAAGGAC+AGG + chr2_4:61994654-61994673 Msa0340650:intron 45.0%
! AAATCGCTGTTTCGTGGCAT+AGG + chr2_4:61994894-61994913 Msa0340650:intron 45.0%
! AATCGCTGTTTCGTGGCATA+GGG + chr2_4:61994895-61994914 Msa0340650:intron 45.0%
!! AAAAGAGCACCACAAAACCG+GGG + chr2_4:61994961-61994980 Msa0340650:CDS 45.0%
ATATACTCCATCTCGCAGCT+AGG + chr2_4:61995061-61995080 Msa0340650:CDS 45.0%
TATACTCCATCTCGCAGCTA+GGG + chr2_4:61995062-61995081 Msa0340650:CDS 45.0%
AGCTAGGGATGCTGCTAAAA+TGG + chr2_4:61995077-61995096 Msa0340650:CDS 45.0%
! GAGAAGATTTTTCTGCGGAG+GGG - chr2_4:61995142-61995161 None:intergenic 45.0%
! GAGAGAAGATTTTTCTGCGG+AGG - chr2_4:61995144-61995163 None:intergenic 45.0%
CCTGTGAAAACTGGATAACC+TGG - chr2_4:61995232-61995251 None:intergenic 45.0%
! CCAGGTTATCCAGTTTTCAC+AGG + chr2_4:61995229-61995248 Msa0340650:CDS 45.0%
TAGAGATTCCTGCCATGAGA+TGG - chr2_4:61995308-61995327 None:intergenic 45.0%
CGAAAAGTAGCATCCATGCT+TGG - chr2_4:61995542-61995561 None:intergenic 45.0%
!! CTACTTTTCGAGACACGTTG+AGG + chr2_4:61995552-61995571 Msa0340650:CDS 45.0%
!! ACTTTTCGAGACACGTTGAG+GGG + chr2_4:61995554-61995573 Msa0340650:CDS 45.0%
TGTCCCCCAAGAACAAAGAA+AGG + chr2_4:61995581-61995600 Msa0340650:CDS 45.0%
CTGGGTTTAGGTATACCAGT+GGG - chr2_4:61995616-61995635 None:intergenic 45.0%
TCTGGGTTTAGGTATACCAG+TGG - chr2_4:61995617-61995636 None:intergenic 45.0%
GTATACCTAAACCCAGACTC+TGG + chr2_4:61995620-61995639 Msa0340650:CDS 45.0%
! CTTTTCCAGAGTCTGGGTTT+AGG - chr2_4:61995628-61995647 None:intergenic 45.0%
GGCACCAAGTGAAGATTCAC+TGG + chr2_4:61994433-61994452 Msa0340650:CDS 50.0%
!! AAAGAGCACCACAAAACCGG+GGG + chr2_4:61994962-61994981 Msa0340650:CDS 50.0%
GAGATGGAGTATATCAGCGC+AGG - chr2_4:61995055-61995074 None:intergenic 50.0%
!! ATGAGATGGATGGTGATGGC+AGG - chr2_4:61995294-61995313 None:intergenic 50.0%
TGCCATCACCATCCATCTCA+TGG + chr2_4:61995293-61995312 Msa0340650:CDS 50.0%
TGCCATGAGATGGATGGTGA+TGG - chr2_4:61995298-61995317 None:intergenic 50.0%
ATCACCATCCATCTCATGGC+AGG + chr2_4:61995297-61995316 Msa0340650:CDS 50.0%
GATTCCTGCCATGAGATGGA+TGG - chr2_4:61995304-61995323 None:intergenic 50.0%
! AACACAGGAGCACACACACT+AGG + chr2_4:61995452-61995471 Msa0340650:intron 50.0%
TGGAAACTCAGACCCAAGCA+TGG + chr2_4:61995526-61995545 Msa0340650:CDS 50.0%
GAAAGGACAAAGTGACCCAC+TGG + chr2_4:61995598-61995617 Msa0340650:CDS 50.0%
TGACACCAAAGAGATCACCG+AGG + chr2_4:61995733-61995752 Msa0340650:CDS 50.0%
!! ATCACCGAGGAATTTGCTGC+TGG + chr2_4:61995746-61995765 Msa0340650:CDS 50.0%
! GAAATCAAGGAGAGGTACGC+CGG + chr2_4:61995837-61995856 Msa0340650:CDS 50.0%
GGCACCAAGTGAAGATTCAC+TGG + chr2_4:61994433-61994452 Msa0340650:CDS 50.0%
!! AAAGAGCACCACAAAACCGG+GGG + chr2_4:61994962-61994981 Msa0340650:CDS 50.0%
GAGATGGAGTATATCAGCGC+AGG - chr2_4:61995055-61995074 None:intergenic 50.0%
!! ATGAGATGGATGGTGATGGC+AGG - chr2_4:61995294-61995313 None:intergenic 50.0%
TGCCATCACCATCCATCTCA+TGG + chr2_4:61995293-61995312 Msa0340650:CDS 50.0%
TGCCATGAGATGGATGGTGA+TGG - chr2_4:61995298-61995317 None:intergenic 50.0%
ATCACCATCCATCTCATGGC+AGG + chr2_4:61995297-61995316 Msa0340650:CDS 50.0%
GATTCCTGCCATGAGATGGA+TGG - chr2_4:61995304-61995323 None:intergenic 50.0%
! AACACAGGAGCACACACACT+AGG + chr2_4:61995452-61995471 Msa0340650:intron 50.0%
TGGAAACTCAGACCCAAGCA+TGG + chr2_4:61995526-61995545 Msa0340650:CDS 50.0%
GAAAGGACAAAGTGACCCAC+TGG + chr2_4:61995598-61995617 Msa0340650:CDS 50.0%
TGACACCAAAGAGATCACCG+AGG + chr2_4:61995733-61995752 Msa0340650:CDS 50.0%
!! ATCACCGAGGAATTTGCTGC+TGG + chr2_4:61995746-61995765 Msa0340650:CDS 50.0%
! GAAATCAAGGAGAGGTACGC+CGG + chr2_4:61995837-61995856 Msa0340650:CDS 50.0%
! GCAGTGTTGAGCTTGTGCCA+TGG + chr2_4:61994330-61994349 Msa0340650:CDS 55.0%
GTACAAATCCTCCAAGCCCC+CGG - chr2_4:61994981-61995000 None:intergenic 55.0%
!! GTTCTGGAATCACGATGCCC+TGG + chr2_4:61995021-61995040 Msa0340650:CDS 55.0%
CAGCGCAGGAGACAATTCCA+GGG - chr2_4:61995041-61995060 None:intergenic 55.0%
TCAGCGCAGGAGACAATTCC+AGG - chr2_4:61995042-61995061 None:intergenic 55.0%
TTCTGCGGAGGGGATAGTGA+AGG - chr2_4:61995132-61995151 None:intergenic 55.0%
CTGCGCAATGAAGCTGTCCT+GGG + chr2_4:61995683-61995702 Msa0340650:CDS 55.0%
! GCAGTGTTGAGCTTGTGCCA+TGG + chr2_4:61994330-61994349 Msa0340650:CDS 55.0%
GTACAAATCCTCCAAGCCCC+CGG - chr2_4:61994981-61995000 None:intergenic 55.0%
!! GTTCTGGAATCACGATGCCC+TGG + chr2_4:61995021-61995040 Msa0340650:CDS 55.0%
CAGCGCAGGAGACAATTCCA+GGG - chr2_4:61995041-61995060 None:intergenic 55.0%
TCAGCGCAGGAGACAATTCC+AGG - chr2_4:61995042-61995061 None:intergenic 55.0%
TTCTGCGGAGGGGATAGTGA+AGG - chr2_4:61995132-61995151 None:intergenic 55.0%
CTGCGCAATGAAGCTGTCCT+GGG + chr2_4:61995683-61995702 Msa0340650:CDS 55.0%
CAATGTATGCCGCGACGCAG+AGG + chr2_4:61994401-61994420 Msa0340650:CDS 60.0%
GGACATACACCTCTGCGTCG+CGG - chr2_4:61994413-61994432 None:intergenic 60.0%
! GCGACGCAGAGGTGTATGTC+CGG + chr2_4:61994412-61994431 Msa0340650:CDS 60.0%
CAGCATCCCTAGCTGCGAGA+TGG - chr2_4:61995071-61995090 None:intergenic 60.0%
TGTCAACAGGCAGGTGCACC+AGG + chr2_4:61995211-61995230 Msa0340650:intron 60.0%
CCAGGACAGCTTCATTGCGC+AGG - chr2_4:61995685-61995704 None:intergenic 60.0%
CCTGCGCAATGAAGCTGTCC+TGG + chr2_4:61995682-61995701 Msa0340650:CDS 60.0%
CAATGTATGCCGCGACGCAG+AGG + chr2_4:61994401-61994420 Msa0340650:CDS 60.0%
GGACATACACCTCTGCGTCG+CGG - chr2_4:61994413-61994432 None:intergenic 60.0%
! GCGACGCAGAGGTGTATGTC+CGG + chr2_4:61994412-61994431 Msa0340650:CDS 60.0%
CAGCATCCCTAGCTGCGAGA+TGG - chr2_4:61995071-61995090 None:intergenic 60.0%
TGTCAACAGGCAGGTGCACC+AGG + chr2_4:61995211-61995230 Msa0340650:intron 60.0%
CCAGGACAGCTTCATTGCGC+AGG - chr2_4:61995685-61995704 None:intergenic 60.0%
CCTGCGCAATGAAGCTGTCC+TGG + chr2_4:61995682-61995701 Msa0340650:CDS 60.0%
GAGCTGCTGCTGCTGCTCCA+TGG - chr2_4:61994350-61994369 None:intergenic 65.0%
GCACCACAAAACCGGGGGCT+TGG + chr2_4:61994967-61994986 Msa0340650:CDS 65.0%
! CCTCCAAGCCCCCGGTTTTG+TGG - chr2_4:61994973-61994992 None:intergenic 65.0%
CCACAAAACCGGGGGCTTGG+AGG + chr2_4:61994970-61994989 Msa0340650:CDS 65.0%
GAGCTGCTGCTGCTGCTCCA+TGG - chr2_4:61994350-61994369 None:intergenic 65.0%
GCACCACAAAACCGGGGGCT+TGG + chr2_4:61994967-61994986 Msa0340650:CDS 65.0%
! CCTCCAAGCCCCCGGTTTTG+TGG - chr2_4:61994973-61994992 None:intergenic 65.0%
CCACAAAACCGGGGGCTTGG+AGG + chr2_4:61994970-61994989 Msa0340650:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 61994285 61995883 61994285 ID=Msa0340650;Name=Msa0340650
chr2_4 mRNA 61994285 61995883 61994285 ID=Msa0340650-mRNA-1;Parent=Msa0340650;Name=Msa0340650-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|4|0|333
chr2_4 exon 61994285 61994518 61994285 ID=Msa0340650-mRNA-1:exon:19656;Parent=Msa0340650-mRNA-1
chr2_4 exon 61994916 61995098 61994916 ID=Msa0340650-mRNA-1:exon:19657;Parent=Msa0340650-mRNA-1
chr2_4 exon 61995220 61995379 61995220 ID=Msa0340650-mRNA-1:exon:19658;Parent=Msa0340650-mRNA-1
chr2_4 exon 61995459 61995883 61995459 ID=Msa0340650-mRNA-1:exon:19659;Parent=Msa0340650-mRNA-1
chr2_4 CDS 61994285 61994518 61994285 ID=Msa0340650-mRNA-1:cds;Parent=Msa0340650-mRNA-1
chr2_4 CDS 61994916 61995098 61994916 ID=Msa0340650-mRNA-1:cds;Parent=Msa0340650-mRNA-1
chr2_4 CDS 61995220 61995379 61995220 ID=Msa0340650-mRNA-1:cds;Parent=Msa0340650-mRNA-1
chr2_4 CDS 61995459 61995883 61995459 ID=Msa0340650-mRNA-1:cds;Parent=Msa0340650-mRNA-1
Gene Sequence

>Msa0340650

ATGGGTAGACTTATGTATGTGGCTTTCCCACTAATAATAGCTTTTGCAGTGTTGAGCTTGTGCCATGGAGCAGCAGCAGCAGCTCCAAAGCCAAAGTTGCAATGGCATCACTATCACAATGTATGCCGCGACGCAGAGGTGTATGTCCGGCACCAAGTGAAGATTCACTGGGATCAAGACAAAAGTATTACTGCCAAGTTACTACGCTTGGTTTACACCGACTGTTTTGTCAATGGTTGTGATGCATCAATACTGCTTGATGAAGGACCAAATCCAGAAAAAAGAGCACCACAAAACCGGGGGCTTGGAGGATTTGTACTAATTGACAAGATCAAAACTGTTCTGGAATCACGATGCCCTGGAATTGTCTCCTGCGCTGATATACTCCATCTCGCAGCTAGGGATGCTGCTAAAATGGCAGGTGCACCAGGTTATCCAGTTTTCACAGGAAGAAAGGATGGCATGAAATCAGATGCTGCATCAGTAGACCTGCCATCACCATCCATCTCATGGCAGGAATCTCTAGCATACTTTAAATCAAGGGGACTGGACGTATTAGATATGACAACACTCTTAGGAGCACACACACTAGGCCAAACACATTGTAGCCACATTGTTGACAGGCTGTATAATTACAATGGTACTGGAAACTCAGACCCAAGCATGGATGCTACTTTTCGAGACACGTTGAGGGGACTTTGTCCCCCAAGAACAAAGAAAGGACAAAGTGACCCACTGGTATACCTAAACCCAGACTCTGGAAAAGATTACATCTTCAGAGAATCATACTATAAAAGGATCCTGCGCAATGAAGCTGTCCTGGGAATCGATCAACAATTACTATTTGGTGATGACACCAAAGAGATCACCGAGGAATTTGCTGCTGGATTTGAAGATTTTAGGAGATCTTTTGCTCAATCCATGTTCAAAATGGGAAATATCAAAGTTTTAACAGGAAATCAAGGAGAGGTACGCCGGAGTTGTCGATACACAAATAAGTGA
Protein sequence

>Msa0340650

MGRLMYVAFPLIIAFAVLSLCHGAAAAAPKPKLQWHHYHNVCRDAEVYVRHQVKIHWDQDKSITAKLLRLVYTDCFVNGCDASILLDEGPNPEKRAPQNRGLGGFVLIDKIKTVLESRCPGIVSCADILHLAARDAAKMAGAPGYPVFTGRKDGMKSDAASVDLPSPSISWQESLAYFKSRGLDVLDMTTLLGAHTLGQTHCSHIVDRLYNYNGTGNSDPSMDATFRDTLRGLCPPRTKKGQSDPLVYLNPDSGKDYIFRESYYKRILRNEAVLGIDQQLLFGDDTKEITEEFAAGFEDFRRSFAQSMFKMGNIKVLTGNQGEVRRSCRYTNK*