AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0313050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313050 MtrunA17_Chr2g0282561 95.614 114 3 1 1 114 1 112 9.82e-75 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313050 AT1G28330.1 59.504 121 41 5 1 114 3 122 2.66e-38 125
Msa0313050 AT1G28330.4 58.678 121 42 5 1 114 3 122 1.85e-37 123
Msa0313050 AT2G33830.2 59.649 114 36 5 5 114 1 108 2.48e-37 122
Msa0313050 AT2G33830.1 57.143 112 40 4 5 114 1 106 1.61e-35 117
Msa0313050 AT5G44300.1 55.172 116 46 4 1 114 3 114 2.35e-35 117
Msa0313050 AT1G28330.5 58.182 110 38 5 1 103 3 111 1.50e-30 105
Msa0313050 AT1G28330.6 58.182 110 38 5 1 103 3 111 1.50e-30 105
Msa0313050 AT1G28330.3 54.167 120 47 5 1 113 3 121 1.96e-30 105
Msa0313050 AT1G28330.2 54.167 120 47 5 1 113 3 121 1.96e-30 105

Find 55 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGTTTGTCTAACATTT+TGG 0.088107 2_4:-8324497 None:intergenic
GATGTGCCACCAAAACTTTC+TGG 0.166653 2_4:+8325335 Msa0313050:exon
TTAGCTAGATAAACCTATTA+AGG 0.299960 2_4:-8325528 None:intergenic
CCAAAATGTTAGACAAACTT+TGG 0.309017 2_4:+8324497 Msa0313050:exon
TGGAGGAGCGTGTTCAATCC+TGG 0.327841 2_4:+8324863 Msa0313050:CDS
TTATCTAGCTTTGCCTTAAT+AGG 0.332499 2_4:+8325515 Msa0313050:three_prime_UTR
CACGTAAAGTTGACAACGTT+TGG 0.347744 2_4:+8324843 Msa0313050:CDS
GCAATCAACCCAGAAAGTTT+TGG 0.376912 2_4:-8325344 None:intergenic
TCAAGGCCACGCTCAGGTTG+AGG 0.384188 2_4:-8324538 None:intergenic
CAAAATGTTAGACAAACTTT+GGG 0.385846 2_4:+8324498 Msa0313050:exon
CTTTGGGATGATACCGTTGC+TGG 0.399829 2_4:+8324514 Msa0313050:CDS
AACTCTGCTACTAAGACAAT+TGG 0.426460 2_4:+8324890 Msa0313050:CDS
GTTTATACCTTTGATGATGC+TGG 0.439938 2_4:-8324581 None:intergenic
ATTCCATCTTGGTAATAGCT+TGG 0.452319 2_4:+8325687 Msa0313050:three_prime_UTR
CAAGGCCACGCTCAGGTTGA+GGG 0.457180 2_4:-8324537 None:intergenic
ATGTGCCACCAAAACTTTCT+GGG 0.468880 2_4:+8325336 Msa0313050:exon
GATGGAATTGCACAGTTACA+TGG 0.475449 2_4:-8325672 None:intergenic
GGAGGAGCGTGTTCAATCCT+GGG 0.490368 2_4:+8324864 Msa0313050:CDS
TGGCAGACGATCAACAAAAC+AGG 0.493537 2_4:-8325380 None:intergenic
GATCAGATCACGACACGTAA+TGG 0.493690 2_4:-8325416 None:intergenic
TCAACTTTACGTGCCGATTG+AGG 0.495412 2_4:-8324833 None:intergenic
GACTCCAACGACACCTCAAT+CGG 0.504134 2_4:+8324820 Msa0313050:CDS
AGCTTCTCAAGGCCACGCTC+AGG 0.504351 2_4:-8324544 None:intergenic
ACCGGTGTTGTCGGTGTCGA+AGG 0.505007 2_4:-8324797 None:intergenic
TGAGGTGTCGTTGGAGTCAC+CGG 0.510899 2_4:-8324815 None:intergenic
TTGTATTTAGGTGTAGATGA+AGG 0.513685 2_4:+8324740 Msa0313050:intron
GTGATCGCAGGATGTACAGC+GGG 0.519197 2_4:+8325290 Msa0313050:intron
TTACGTGTCGTGATCTGATC+GGG 0.520466 2_4:+8325419 Msa0313050:three_prime_UTR
CTTCTGAATATTGTAACAAA+CGG 0.522502 2_4:+8325624 Msa0313050:three_prime_UTR
GTAACTGTGCAATTCCATCT+TGG 0.524557 2_4:+8325676 Msa0313050:three_prime_UTR
GTTGGAGTCACCGGTGTTGT+CGG 0.528758 2_4:-8324806 None:intergenic
TTAGTAGCAGAGTTACTCCC+AGG 0.531237 2_4:-8324881 None:intergenic
AGCGTGGCCTTGAGAAGCTT+AGG 0.531934 2_4:+8324548 Msa0313050:CDS
GGATGTACAGCGGGGACACA+AGG 0.536816 2_4:+8325299 Msa0313050:intron
CGTGCCGATTGAGGTGTCGT+TGG 0.538925 2_4:-8324824 None:intergenic
AATCCAAGCTATTACCAAGA+TGG 0.547872 2_4:-8325690 None:intergenic
ATTACGTGTCGTGATCTGAT+CGG 0.563489 2_4:+8325418 Msa0313050:three_prime_UTR
ACTAAGATGGAACTAAGACT+TGG 0.571418 2_4:-8324473 None:intergenic
TATTTAGGTGTAGATGAAGG+AGG 0.572856 2_4:+8324743 Msa0313050:intron
CTCAGGTTGAGGGCCAGCAA+CGG 0.587677 2_4:-8324527 None:intergenic
GTAAAGTTGACAACGTTTGG+AGG 0.589302 2_4:+8324846 Msa0313050:CDS
GCTGGCCCTCAACCTGAGCG+TGG 0.589408 2_4:+8324532 Msa0313050:CDS
ACATACCAATCATAGACAGT+GGG 0.591016 2_4:-8324947 None:intergenic
TTAGCTTCCTAAGCTTCTCA+AGG 0.592667 2_4:-8324555 None:intergenic
GCCTTCGACACCGACAACAC+CGG 0.604941 2_4:+8324796 Msa0313050:CDS
TATACCTTTGATGATGCTGG+TGG 0.618859 2_4:-8324578 None:intergenic
CTAACCACCAGCATCATCAA+AGG 0.620330 2_4:+8324574 Msa0313050:CDS
ACAAAACTCAAATTAATACA+AGG 0.623205 2_4:-8325493 None:intergenic
GATTAATTAACACTTACAGA+AGG 0.629797 2_4:-8325564 None:intergenic
AACATACCAATCATAGACAG+TGG 0.664521 2_4:-8324948 None:intergenic
CATACCAATCATAGACAGTG+GGG 0.727901 2_4:-8324946 None:intergenic
TGTGATCGCAGGATGTACAG+CGG 0.734662 2_4:+8325289 Msa0313050:intron
ATACCAATCATAGACAGTGG+GGG 0.749000 2_4:-8324945 None:intergenic
TGATCGCAGGATGTACAGCG+GGG 0.761714 2_4:+8325291 Msa0313050:intron
GTAATGGTGATACAGACACG+TGG 0.778111 2_4:-8325400 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTCATTATATTATAGT+TGG - chr2_4:8324636-8324655 None:intergenic 10.0%
!!! ATTCATTTTAATTTGTATTT+AGG + chr2_4:8324728-8324747 Msa0313050:intron 10.0%
!!! TTGATTCTATATTTTATTTT+TGG + chr2_4:8325081-8325100 Msa0313050:intron 10.0%
!!! TTAAGAATTTTATATTACAA+AGG + chr2_4:8325714-8325733 Msa0313050:three_prime_UTR 10.0%
!!! TAAGAATTTTATATTACAAA+GGG + chr2_4:8325715-8325734 Msa0313050:three_prime_UTR 10.0%
!! TATATTCATTATATTATAGT+TGG - chr2_4:8324636-8324655 None:intergenic 10.0%
!!! ATTCATTTTAATTTGTATTT+AGG + chr2_4:8324728-8324747 Msa0313050:intron 10.0%
!!! TTGATTCTATATTTTATTTT+TGG + chr2_4:8325081-8325100 Msa0313050:intron 10.0%
!!! TTAAGAATTTTATATTACAA+AGG + chr2_4:8325714-8325733 Msa0313050:three_prime_UTR 10.0%
!!! TAAGAATTTTATATTACAAA+GGG + chr2_4:8325715-8325734 Msa0313050:three_prime_UTR 10.0%
!! ACAAACATATAAAAATGAAT+TGG + chr2_4:8325260-8325279 Msa0313050:intron 15.0%
!! CAAACATATAAAAATGAATT+GGG + chr2_4:8325261-8325280 Msa0313050:intron 15.0%
!!! AAGAATTTTATATTACAAAG+GGG + chr2_4:8325716-8325735 Msa0313050:three_prime_UTR 15.0%
!! ACAAACATATAAAAATGAAT+TGG + chr2_4:8325260-8325279 Msa0313050:intron 15.0%
!! CAAACATATAAAAATGAATT+GGG + chr2_4:8325261-8325280 Msa0313050:intron 15.0%
!!! AAGAATTTTATATTACAAAG+GGG + chr2_4:8325716-8325735 Msa0313050:three_prime_UTR 15.0%
!! ACAAAACTCAAATTAATACA+AGG - chr2_4:8325496-8325515 None:intergenic 20.0%
!! AATATTCAGAAGAAGAAAAA+AGG - chr2_4:8325616-8325635 None:intergenic 20.0%
!! ACAAAACTCAAATTAATACA+AGG - chr2_4:8325496-8325515 None:intergenic 20.0%
!! AATATTCAGAAGAAGAAAAA+AGG - chr2_4:8325616-8325635 None:intergenic 20.0%
! CAAAATGTTAGACAAACTTT+GGG + chr2_4:8324498-8324517 Msa0313050:exon 25.0%
! TGTATAGTTCTTCTAACAAA+AGG - chr2_4:8325149-8325168 None:intergenic 25.0%
! TTAGCTAGATAAACCTATTA+AGG - chr2_4:8325531-8325550 None:intergenic 25.0%
! GATTAATTAACACTTACAGA+AGG - chr2_4:8325567-8325586 None:intergenic 25.0%
! CTTCTGAATATTGTAACAAA+CGG + chr2_4:8325624-8325643 Msa0313050:three_prime_UTR 25.0%
! CAAAATGTTAGACAAACTTT+GGG + chr2_4:8324498-8324517 Msa0313050:exon 25.0%
! TGTATAGTTCTTCTAACAAA+AGG - chr2_4:8325149-8325168 None:intergenic 25.0%
! TTAGCTAGATAAACCTATTA+AGG - chr2_4:8325531-8325550 None:intergenic 25.0%
! GATTAATTAACACTTACAGA+AGG - chr2_4:8325567-8325586 None:intergenic 25.0%
! CTTCTGAATATTGTAACAAA+CGG + chr2_4:8325624-8325643 Msa0313050:three_prime_UTR 25.0%
!!! CTAACATTTTGGAACTAAGA+TGG - chr2_4:8324489-8324508 None:intergenic 30.0%
! CCAAAGTTTGTCTAACATTT+TGG - chr2_4:8324500-8324519 None:intergenic 30.0%
CCAAAATGTTAGACAAACTT+TGG + chr2_4:8324497-8324516 Msa0313050:exon 30.0%
TTGTATTTAGGTGTAGATGA+AGG + chr2_4:8324740-8324759 Msa0313050:intron 30.0%
TGTTAGAAGAACTATACATG+TGG + chr2_4:8325151-8325170 Msa0313050:intron 30.0%
!! TTATCTAGCTTTGCCTTAAT+AGG + chr2_4:8325515-8325534 Msa0313050:three_prime_UTR 30.0%
!!! CTAACATTTTGGAACTAAGA+TGG - chr2_4:8324489-8324508 None:intergenic 30.0%
! CCAAAGTTTGTCTAACATTT+TGG - chr2_4:8324500-8324519 None:intergenic 30.0%
CCAAAATGTTAGACAAACTT+TGG + chr2_4:8324497-8324516 Msa0313050:exon 30.0%
TTGTATTTAGGTGTAGATGA+AGG + chr2_4:8324740-8324759 Msa0313050:intron 30.0%
TGTTAGAAGAACTATACATG+TGG + chr2_4:8325151-8325170 Msa0313050:intron 30.0%
!! TTATCTAGCTTTGCCTTAAT+AGG + chr2_4:8325515-8325534 Msa0313050:three_prime_UTR 30.0%
ACTAAGATGGAACTAAGACT+TGG - chr2_4:8324476-8324495 None:intergenic 35.0%
!! GTTTATACCTTTGATGATGC+TGG - chr2_4:8324584-8324603 None:intergenic 35.0%
TATTTAGGTGTAGATGAAGG+AGG + chr2_4:8324743-8324762 Msa0313050:intron 35.0%
AACTCTGCTACTAAGACAAT+TGG + chr2_4:8324890-8324909 Msa0313050:CDS 35.0%
ACATACCAATCATAGACAGT+GGG - chr2_4:8324950-8324969 None:intergenic 35.0%
AACATACCAATCATAGACAG+TGG - chr2_4:8324951-8324970 None:intergenic 35.0%
ACGCAAATTATAAGAGTGGT+TGG - chr2_4:8324991-8325010 None:intergenic 35.0%
ATGCACGCAAATTATAAGAG+TGG - chr2_4:8324995-8325014 None:intergenic 35.0%
! ATTCCATCTTGGTAATAGCT+TGG + chr2_4:8325687-8325706 Msa0313050:three_prime_UTR 35.0%
AATCCAAGCTATTACCAAGA+TGG - chr2_4:8325693-8325712 None:intergenic 35.0%
ACTAAGATGGAACTAAGACT+TGG - chr2_4:8324476-8324495 None:intergenic 35.0%
!! GTTTATACCTTTGATGATGC+TGG - chr2_4:8324584-8324603 None:intergenic 35.0%
TATTTAGGTGTAGATGAAGG+AGG + chr2_4:8324743-8324762 Msa0313050:intron 35.0%
AACTCTGCTACTAAGACAAT+TGG + chr2_4:8324890-8324909 Msa0313050:CDS 35.0%
ACATACCAATCATAGACAGT+GGG - chr2_4:8324950-8324969 None:intergenic 35.0%
AACATACCAATCATAGACAG+TGG - chr2_4:8324951-8324970 None:intergenic 35.0%
ACGCAAATTATAAGAGTGGT+TGG - chr2_4:8324991-8325010 None:intergenic 35.0%
ATGCACGCAAATTATAAGAG+TGG - chr2_4:8324995-8325014 None:intergenic 35.0%
! ATTCCATCTTGGTAATAGCT+TGG + chr2_4:8325687-8325706 Msa0313050:three_prime_UTR 35.0%
AATCCAAGCTATTACCAAGA+TGG - chr2_4:8325693-8325712 None:intergenic 35.0%
TTAGCTTCCTAAGCTTCTCA+AGG - chr2_4:8324558-8324577 None:intergenic 40.0%
!! TATACCTTTGATGATGCTGG+TGG - chr2_4:8324581-8324600 None:intergenic 40.0%
CACGTAAAGTTGACAACGTT+TGG + chr2_4:8324843-8324862 Msa0313050:CDS 40.0%
GTAAAGTTGACAACGTTTGG+AGG + chr2_4:8324846-8324865 Msa0313050:CDS 40.0%
ATACCAATCATAGACAGTGG+GGG - chr2_4:8324948-8324967 None:intergenic 40.0%
CATACCAATCATAGACAGTG+GGG - chr2_4:8324949-8324968 None:intergenic 40.0%
ATGTGCCACCAAAACTTTCT+GGG + chr2_4:8325336-8325355 Msa0313050:exon 40.0%
!! ATCAACCCAGAAAGTTTTGG+TGG - chr2_4:8325344-8325363 None:intergenic 40.0%
! GCAATCAACCCAGAAAGTTT+TGG - chr2_4:8325347-8325366 None:intergenic 40.0%
ATTACGTGTCGTGATCTGAT+CGG + chr2_4:8325418-8325437 Msa0313050:three_prime_UTR 40.0%
GATGGAATTGCACAGTTACA+TGG - chr2_4:8325675-8325694 None:intergenic 40.0%
GTAACTGTGCAATTCCATCT+TGG + chr2_4:8325676-8325695 Msa0313050:three_prime_UTR 40.0%
TTAGCTTCCTAAGCTTCTCA+AGG - chr2_4:8324558-8324577 None:intergenic 40.0%
!! TATACCTTTGATGATGCTGG+TGG - chr2_4:8324581-8324600 None:intergenic 40.0%
CACGTAAAGTTGACAACGTT+TGG + chr2_4:8324843-8324862 Msa0313050:CDS 40.0%
GTAAAGTTGACAACGTTTGG+AGG + chr2_4:8324846-8324865 Msa0313050:CDS 40.0%
ATACCAATCATAGACAGTGG+GGG - chr2_4:8324948-8324967 None:intergenic 40.0%
CATACCAATCATAGACAGTG+GGG - chr2_4:8324949-8324968 None:intergenic 40.0%
ATGTGCCACCAAAACTTTCT+GGG + chr2_4:8325336-8325355 Msa0313050:exon 40.0%
!! ATCAACCCAGAAAGTTTTGG+TGG - chr2_4:8325344-8325363 None:intergenic 40.0%
! GCAATCAACCCAGAAAGTTT+TGG - chr2_4:8325347-8325366 None:intergenic 40.0%
ATTACGTGTCGTGATCTGAT+CGG + chr2_4:8325418-8325437 Msa0313050:three_prime_UTR 40.0%
GATGGAATTGCACAGTTACA+TGG - chr2_4:8325675-8325694 None:intergenic 40.0%
GTAACTGTGCAATTCCATCT+TGG + chr2_4:8325676-8325695 Msa0313050:three_prime_UTR 40.0%
CTAACCACCAGCATCATCAA+AGG + chr2_4:8324574-8324593 Msa0313050:CDS 45.0%
!! TCAACTTTACGTGCCGATTG+AGG - chr2_4:8324836-8324855 None:intergenic 45.0%
TTAGTAGCAGAGTTACTCCC+AGG - chr2_4:8324884-8324903 None:intergenic 45.0%
! ATTGGGTTGTATGTGATCGC+AGG + chr2_4:8325278-8325297 Msa0313050:intron 45.0%
!! AAGTTTTGGTGGCACATCAG+CGG - chr2_4:8325333-8325352 None:intergenic 45.0%
GATGTGCCACCAAAACTTTC+TGG + chr2_4:8325335-8325354 Msa0313050:exon 45.0%
TGGCAGACGATCAACAAAAC+AGG - chr2_4:8325383-8325402 None:intergenic 45.0%
GTAATGGTGATACAGACACG+TGG - chr2_4:8325403-8325422 None:intergenic 45.0%
GATCAGATCACGACACGTAA+TGG - chr2_4:8325419-8325438 None:intergenic 45.0%
TTACGTGTCGTGATCTGATC+GGG + chr2_4:8325419-8325438 Msa0313050:three_prime_UTR 45.0%
CTAACCACCAGCATCATCAA+AGG + chr2_4:8324574-8324593 Msa0313050:CDS 45.0%
!! TCAACTTTACGTGCCGATTG+AGG - chr2_4:8324836-8324855 None:intergenic 45.0%
TTAGTAGCAGAGTTACTCCC+AGG - chr2_4:8324884-8324903 None:intergenic 45.0%
! ATTGGGTTGTATGTGATCGC+AGG + chr2_4:8325278-8325297 Msa0313050:intron 45.0%
!! AAGTTTTGGTGGCACATCAG+CGG - chr2_4:8325333-8325352 None:intergenic 45.0%
GATGTGCCACCAAAACTTTC+TGG + chr2_4:8325335-8325354 Msa0313050:exon 45.0%
TGGCAGACGATCAACAAAAC+AGG - chr2_4:8325383-8325402 None:intergenic 45.0%
GTAATGGTGATACAGACACG+TGG - chr2_4:8325403-8325422 None:intergenic 45.0%
GATCAGATCACGACACGTAA+TGG - chr2_4:8325419-8325438 None:intergenic 45.0%
TTACGTGTCGTGATCTGATC+GGG + chr2_4:8325419-8325438 Msa0313050:three_prime_UTR 45.0%
!! CTTTGGGATGATACCGTTGC+TGG + chr2_4:8324514-8324533 Msa0313050:CDS 50.0%
GACTCCAACGACACCTCAAT+CGG + chr2_4:8324820-8324839 Msa0313050:CDS 50.0%
!! TCATAGACAGTGGGGGTGTT+GGG - chr2_4:8324941-8324960 None:intergenic 50.0%
! ATCATAGACAGTGGGGGTGT+TGG - chr2_4:8324942-8324961 None:intergenic 50.0%
ACACCCCCACTGTCTATGAT+TGG + chr2_4:8324942-8324961 Msa0313050:CDS 50.0%
TGTGATCGCAGGATGTACAG+CGG + chr2_4:8325289-8325308 Msa0313050:intron 50.0%
!! CTTTGGGATGATACCGTTGC+TGG + chr2_4:8324514-8324533 Msa0313050:CDS 50.0%
GACTCCAACGACACCTCAAT+CGG + chr2_4:8324820-8324839 Msa0313050:CDS 50.0%
!! TCATAGACAGTGGGGGTGTT+GGG - chr2_4:8324941-8324960 None:intergenic 50.0%
! ATCATAGACAGTGGGGGTGT+TGG - chr2_4:8324942-8324961 None:intergenic 50.0%
ACACCCCCACTGTCTATGAT+TGG + chr2_4:8324942-8324961 Msa0313050:CDS 50.0%
TGTGATCGCAGGATGTACAG+CGG + chr2_4:8325289-8325308 Msa0313050:intron 50.0%
! AGCGTGGCCTTGAGAAGCTT+AGG + chr2_4:8324548-8324567 Msa0313050:CDS 55.0%
!! GTTGGAGTCACCGGTGTTGT+CGG - chr2_4:8324809-8324828 None:intergenic 55.0%
!! TGAGGTGTCGTTGGAGTCAC+CGG - chr2_4:8324818-8324837 None:intergenic 55.0%
! TGGAGGAGCGTGTTCAATCC+TGG + chr2_4:8324863-8324882 Msa0313050:CDS 55.0%
! GGAGGAGCGTGTTCAATCCT+GGG + chr2_4:8324864-8324883 Msa0313050:CDS 55.0%
!! CATAGACAGTGGGGGTGTTG+GGG - chr2_4:8324940-8324959 None:intergenic 55.0%
GTGATCGCAGGATGTACAGC+GGG + chr2_4:8325290-8325309 Msa0313050:intron 55.0%
TGATCGCAGGATGTACAGCG+GGG + chr2_4:8325291-8325310 Msa0313050:intron 55.0%
! AGCGTGGCCTTGAGAAGCTT+AGG + chr2_4:8324548-8324567 Msa0313050:CDS 55.0%
!! GTTGGAGTCACCGGTGTTGT+CGG - chr2_4:8324809-8324828 None:intergenic 55.0%
!! TGAGGTGTCGTTGGAGTCAC+CGG - chr2_4:8324818-8324837 None:intergenic 55.0%
! TGGAGGAGCGTGTTCAATCC+TGG + chr2_4:8324863-8324882 Msa0313050:CDS 55.0%
! GGAGGAGCGTGTTCAATCCT+GGG + chr2_4:8324864-8324883 Msa0313050:CDS 55.0%
!! CATAGACAGTGGGGGTGTTG+GGG - chr2_4:8324940-8324959 None:intergenic 55.0%
GTGATCGCAGGATGTACAGC+GGG + chr2_4:8325290-8325309 Msa0313050:intron 55.0%
TGATCGCAGGATGTACAGCG+GGG + chr2_4:8325291-8325310 Msa0313050:intron 55.0%
!! CTCAGGTTGAGGGCCAGCAA+CGG - chr2_4:8324530-8324549 None:intergenic 60.0%
!! CAAGGCCACGCTCAGGTTGA+GGG - chr2_4:8324540-8324559 None:intergenic 60.0%
! TCAAGGCCACGCTCAGGTTG+AGG - chr2_4:8324541-8324560 None:intergenic 60.0%
AGCTTCTCAAGGCCACGCTC+AGG - chr2_4:8324547-8324566 None:intergenic 60.0%
GCCTTCGACACCGACAACAC+CGG + chr2_4:8324796-8324815 Msa0313050:CDS 60.0%
!! ACCGGTGTTGTCGGTGTCGA+AGG - chr2_4:8324800-8324819 None:intergenic 60.0%
!! CGTGCCGATTGAGGTGTCGT+TGG - chr2_4:8324827-8324846 None:intergenic 60.0%
GGATGTACAGCGGGGACACA+AGG + chr2_4:8325299-8325318 Msa0313050:intron 60.0%
!! CTCAGGTTGAGGGCCAGCAA+CGG - chr2_4:8324530-8324549 None:intergenic 60.0%
!! CAAGGCCACGCTCAGGTTGA+GGG - chr2_4:8324540-8324559 None:intergenic 60.0%
! TCAAGGCCACGCTCAGGTTG+AGG - chr2_4:8324541-8324560 None:intergenic 60.0%
AGCTTCTCAAGGCCACGCTC+AGG - chr2_4:8324547-8324566 None:intergenic 60.0%
GCCTTCGACACCGACAACAC+CGG + chr2_4:8324796-8324815 Msa0313050:CDS 60.0%
!! ACCGGTGTTGTCGGTGTCGA+AGG - chr2_4:8324800-8324819 None:intergenic 60.0%
!! CGTGCCGATTGAGGTGTCGT+TGG - chr2_4:8324827-8324846 None:intergenic 60.0%
GGATGTACAGCGGGGACACA+AGG + chr2_4:8325299-8325318 Msa0313050:intron 60.0%
!! ACAGTGGGGGTGTTGGGGAG+TGG - chr2_4:8324935-8324954 None:intergenic 65.0%
!! ACAGTGGGGGTGTTGGGGAG+TGG - chr2_4:8324935-8324954 None:intergenic 65.0%
GCTGGCCCTCAACCTGAGCG+TGG + chr2_4:8324532-8324551 Msa0313050:CDS 70.0%
GCTGGCCCTCAACCTGAGCG+TGG + chr2_4:8324532-8324551 Msa0313050:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 8324442 8325739 8324442 ID=Msa0313050;Name=Msa0313050
chr2_4 mRNA 8324442 8325739 8324442 ID=Msa0313050-mRNA-1;Parent=Msa0313050;Name=Msa0313050-mRNA-1;_AED=0.02;_eAED=0.02;_QI=60|1|1|1|1|1|3|403|114
chr2_4 exon 8324442 8324595 8324442 ID=Msa0313050-mRNA-1:exon:3189;Parent=Msa0313050-mRNA-1
chr2_4 exon 8324750 8324963 8324750 ID=Msa0313050-mRNA-1:exon:3190;Parent=Msa0313050-mRNA-1
chr2_4 exon 8325300 8325739 8325300 ID=Msa0313050-mRNA-1:exon:3191;Parent=Msa0313050-mRNA-1
chr2_4 five_prime_UTR 8324442 8324501 8324442 ID=Msa0313050-mRNA-1:five_prime_utr;Parent=Msa0313050-mRNA-1
chr2_4 CDS 8324502 8324595 8324502 ID=Msa0313050-mRNA-1:cds;Parent=Msa0313050-mRNA-1
chr2_4 CDS 8324750 8324963 8324750 ID=Msa0313050-mRNA-1:cds;Parent=Msa0313050-mRNA-1
chr2_4 CDS 8325300 8325336 8325300 ID=Msa0313050-mRNA-1:cds;Parent=Msa0313050-mRNA-1
chr2_4 three_prime_UTR 8325337 8325739 8325337 ID=Msa0313050-mRNA-1:three_prime_utr;Parent=Msa0313050-mRNA-1
Gene Sequence

>Msa0313050

ATGTTAGACAAACTTTGGGATGATACCGTTGCTGGCCCTCAACCTGAGCGTGGCCTTGAGAAGCTTAGGAAGCTAACCACCAGCATCATCAAAGGTGTAGATGAAGGAGGAAGTGCTCAGTTAATGAGAAACACGTCGTTGCCTTCGACACCGACAACACCGGTGACTCCAACGACACCTCAATCGGCACGTAAAGTTGACAACGTTTGGAGGAGCGTGTTCAATCCTGGGAGTAACTCTGCTACTAAGACAATTGGTGCTCATATGTTTGACAAACCACTCCCCAACACCCCCACTGTCTATGATTGGATGTACAGCGGGGACACAAGGAGCAAGCACCGCTGA
Protein sequence

>Msa0313050

MLDKLWDDTVAGPQPERGLEKLRKLTTSIIKGVDEGGSAQLMRNTSLPSTPTTPVTPTTPQSARKVDNVWRSVFNPGSNSATKTIGAHMFDKPLPNTPTVYDWMYSGDTRSKHR*