AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1374140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1374140 MtrunA17_Chr1g0155341 90.210 143 12 1 1 141 1 143 1.72e-92 264
Msa1374140 MtrunA17_Chr3g0141671 62.937 143 44 5 1 140 1 137 1.35e-55 170
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1374140 AT1G19530.2 41.538 130 57 3 1 129 1 112 2.73e-18 75.5
Msa1374140 AT1G19530.1 41.538 130 57 3 1 129 1 112 2.73e-18 75.5

Find 39 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGAAATTGATGCTTAT+TGG 0.156482 tig0019465:+130989 Msa1374140:CDS
AATCATATGGTACCTGATTT+AGG 0.175839 tig0019465:-130810 None:intergenic
CTGAATAGTCTTTGACAAAT+TGG 0.206876 tig0019465:-131055 None:intergenic
GAACGTACTTGACACAAATT+TGG 0.215908 tig0019465:+132119 Msa1374140:CDS
GTCAAGTACGTTCATATTAA+AGG 0.224800 tig0019465:-132109 None:intergenic
CTGTTTCAGCCATACTTAAA+AGG 0.239435 tig0019465:+130950 Msa1374140:intron
GCAAATCAACGTGTGTTTCT+TGG 0.281985 tig0019465:+132599 Msa1374140:three_prime_UTR
CAATTTGTCAAAGACTATTC+AGG 0.285232 tig0019465:+131056 Msa1374140:CDS
ACCACAATGCAATTTCTAAA+TGG 0.294299 tig0019465:-130713 None:intergenic
CAGTTTCACGTTGCCAGTTT+GGG 0.309436 tig0019465:+132389 Msa1374140:CDS
GCAGGTGGACTAAGAGTAAC+TGG 0.334701 tig0019465:+132312 Msa1374140:intron
TCAAGTACGTTCATATTAAA+GGG 0.359264 tig0019465:-132108 None:intergenic
ACAGTTTCACGTTGCCAGTT+TGG 0.359415 tig0019465:+132388 Msa1374140:CDS
TCTTCAATTGATCCTAAATC+AGG 0.362673 tig0019465:+130798 Msa1374140:CDS
TTGGATGCAGCTTCCATCAT+TGG 0.363993 tig0019465:-132353 None:intergenic
GGGTTCTCTTATGGCAGGAT+GGG 0.445491 tig0019465:+130770 Msa1374140:CDS
TCTGAATGAGCCACCAATGA+TGG 0.467236 tig0019465:+132340 Msa1374140:CDS
CCATGCCCGTGGCCCACGTA+AGG 0.471373 tig0019465:+132082 Msa1374140:CDS
GGGTCCCTTACGTGGGCCAC+GGG 0.472849 tig0019465:-132087 None:intergenic
GGGGTCCCTTACGTGGGCCA+CGG 0.474803 tig0019465:-132088 None:intergenic
CCTTACGTGGGCCACGGGCA+TGG 0.492032 tig0019465:-132082 None:intergenic
GGTTCTCTTATGGCAGGATG+GGG 0.492223 tig0019465:+130771 Msa1374140:CDS
CAATCACTAACTTCATCTAC+GGG 0.495529 tig0019465:-132428 None:intergenic
GAAATCAAAGAAGAAGATAG+AGG 0.499005 tig0019465:+131011 Msa1374140:CDS
GATGCAGCTTCCATCATTGG+TGG 0.505474 tig0019465:-132350 None:intergenic
TGGGTTCTCTTATGGCAGGA+TGG 0.507870 tig0019465:+130769 Msa1374140:CDS
ACAAATTGGAAATTGCTGTA+AGG 0.512610 tig0019465:-131041 None:intergenic
TCAATCACTAACTTCATCTA+CGG 0.513170 tig0019465:-132429 None:intergenic
AAACTGTGCTGCATACTTGT+TGG 0.515262 tig0019465:-132372 None:intergenic
TATTAAAGGGGTCCCTTACG+TGG 0.530982 tig0019465:-132095 None:intergenic
AAAATGGGTTCTCTTATGGC+AGG 0.548408 tig0019465:+130765 Msa1374140:exon
CATGCCCGTGGCCCACGTAA+GGG 0.568293 tig0019465:+132083 Msa1374140:CDS
TCCATTTAGAAATTGCATTG+TGG 0.619587 tig0019465:+130712 Msa1374140:five_prime_UTR
GACATAAATATATACATACA+AGG 0.637376 tig0019465:-132478 None:intergenic
GAAGTCAATGACCATGCCCG+TGG 0.649977 tig0019465:+132071 Msa1374140:CDS
CAAGTACGTTCATATTAAAG+GGG 0.651488 tig0019465:-132107 None:intergenic
ATTAAAGGGGTCCCTTACGT+GGG 0.678500 tig0019465:-132094 None:intergenic
CAGGTGGACTAAGAGTAACT+GGG 0.688506 tig0019465:+132313 Msa1374140:intron
AATCACTAACTTCATCTACG+GGG 0.796732 tig0019465:-132427 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTTTGGAAAAATT+TGG - tig0019465:131159-131178 None:intergenic 10.0%
!!! TACAAAATCATATATATTTT+CGG - tig0019465:131831-131850 None:intergenic 10.0%
!! TGAAATAAAAACATTAAAAT+TGG - tig0019465:131972-131991 None:intergenic 10.0%
!!! TTATTTTTTTTGGAAAAATT+TGG - tig0019465:131159-131178 None:intergenic 10.0%
!!! TACAAAATCATATATATTTT+CGG - tig0019465:131831-131850 None:intergenic 10.0%
!! TGAAATAAAAACATTAAAAT+TGG - tig0019465:131972-131991 None:intergenic 10.0%
!! AATAAATAAAATACATACGA+AGG + tig0019465:131174-131193 Msa1374140:intron 15.0%
!!! AAATACATCTTTAATTTCAT+TGG + tig0019465:131332-131351 Msa1374140:intron 15.0%
!! TATAGTATTATATATAGTGT+TGG - tig0019465:131518-131537 None:intergenic 15.0%
!! TTTACACACAATATTAAAAT+TGG - tig0019465:131942-131961 None:intergenic 15.0%
!!! TTAATGTTTTTATTTCATGA+TGG + tig0019465:131975-131994 Msa1374140:intron 15.0%
!! AATAAATAAAATACATACGA+AGG + tig0019465:131174-131193 Msa1374140:intron 15.0%
!!! AAATACATCTTTAATTTCAT+TGG + tig0019465:131332-131351 Msa1374140:intron 15.0%
!! TATAGTATTATATATAGTGT+TGG - tig0019465:131518-131537 None:intergenic 15.0%
!! TTTACACACAATATTAAAAT+TGG - tig0019465:131942-131961 None:intergenic 15.0%
!!! TTAATGTTTTTATTTCATGA+TGG + tig0019465:131975-131994 Msa1374140:intron 15.0%
!! TTGATTCTATACAGAAAAAA+TGG + tig0019465:130749-130768 Msa1374140:exon 20.0%
!! TGATTCTATACAGAAAAAAT+GGG + tig0019465:130750-130769 Msa1374140:exon 20.0%
!!! AAGTATCGTTATTAATCATA+TGG - tig0019465:130826-130845 None:intergenic 20.0%
!! AATCATTATTAGAACTTAGA+CGG + tig0019465:130898-130917 Msa1374140:intron 20.0%
!!! CTACTATTTTCTTTTTCTTT+TGG - tig0019465:131486-131505 None:intergenic 20.0%
!! CTAAACATTAATTTCAAACT+TGG - tig0019465:132015-132034 None:intergenic 20.0%
!!! CAAGTTTGAAATTAATGTTT+AGG + tig0019465:132013-132032 Msa1374140:intron 20.0%
!! CATATGCATAATTCATATAT+AGG - tig0019465:132267-132286 None:intergenic 20.0%
!! CTATATATGAATTATGCATA+TGG + tig0019465:132265-132284 Msa1374140:intron 20.0%
!!! TATTAATTTAATTTGAGTGC+AGG + tig0019465:132294-132313 Msa1374140:intron 20.0%
!! GACATAAATATATACATACA+AGG - tig0019465:132481-132500 None:intergenic 20.0%
!! TTGATTCTATACAGAAAAAA+TGG + tig0019465:130749-130768 Msa1374140:exon 20.0%
!! TGATTCTATACAGAAAAAAT+GGG + tig0019465:130750-130769 Msa1374140:exon 20.0%
!!! AAGTATCGTTATTAATCATA+TGG - tig0019465:130826-130845 None:intergenic 20.0%
!! AATCATTATTAGAACTTAGA+CGG + tig0019465:130898-130917 Msa1374140:intron 20.0%
!!! CTACTATTTTCTTTTTCTTT+TGG - tig0019465:131486-131505 None:intergenic 20.0%
!! CTAAACATTAATTTCAAACT+TGG - tig0019465:132015-132034 None:intergenic 20.0%
!!! CAAGTTTGAAATTAATGTTT+AGG + tig0019465:132013-132032 Msa1374140:intron 20.0%
!! CATATGCATAATTCATATAT+AGG - tig0019465:132267-132286 None:intergenic 20.0%
!! CTATATATGAATTATGCATA+TGG + tig0019465:132265-132284 Msa1374140:intron 20.0%
!!! TATTAATTTAATTTGAGTGC+AGG + tig0019465:132294-132313 Msa1374140:intron 20.0%
!! GACATAAATATATACATACA+AGG - tig0019465:132481-132500 None:intergenic 20.0%
! AAGATGAAATTGATGCTTAT+TGG + tig0019465:130989-131008 Msa1374140:CDS 25.0%
!!! TTTTTTGGAAAAATTTGGTG+TGG - tig0019465:131154-131173 None:intergenic 25.0%
! TGCTCCAATTAACAATTATT+AGG + tig0019465:131241-131260 Msa1374140:intron 25.0%
! GCTCCAATTAACAATTATTA+GGG + tig0019465:131242-131261 Msa1374140:intron 25.0%
! GAACCCTAATAATTGTTAAT+TGG - tig0019465:131248-131267 None:intergenic 25.0%
! TACACTTGAAAAAATCAACA+AGG + tig0019465:131453-131472 Msa1374140:intron 25.0%
! ACACTTGAAAAAATCAACAA+GGG + tig0019465:131454-131473 Msa1374140:intron 25.0%
!!! TATTATATATAGTGTTGGTG+GGG - tig0019465:131513-131532 None:intergenic 25.0%
!!! GTATTATATATAGTGTTGGT+GGG - tig0019465:131514-131533 None:intergenic 25.0%
!! AGTATTATATATAGTGTTGG+TGG - tig0019465:131515-131534 None:intergenic 25.0%
!! TAGTTTTCTAATATGCAAGA+AGG - tig0019465:131770-131789 None:intergenic 25.0%
!! TATATTTTCGGATTTCACTT+AGG - tig0019465:131819-131838 None:intergenic 25.0%
! TCAAGTACGTTCATATTAAA+GGG - tig0019465:132111-132130 None:intergenic 25.0%
! ACTATAATAATTGGAAGAGT+AGG - tig0019465:132179-132198 None:intergenic 25.0%
! AAGATGAAATTGATGCTTAT+TGG + tig0019465:130989-131008 Msa1374140:CDS 25.0%
!!! TTTTTTGGAAAAATTTGGTG+TGG - tig0019465:131154-131173 None:intergenic 25.0%
! TGCTCCAATTAACAATTATT+AGG + tig0019465:131241-131260 Msa1374140:intron 25.0%
! GCTCCAATTAACAATTATTA+GGG + tig0019465:131242-131261 Msa1374140:intron 25.0%
! GAACCCTAATAATTGTTAAT+TGG - tig0019465:131248-131267 None:intergenic 25.0%
! TACACTTGAAAAAATCAACA+AGG + tig0019465:131453-131472 Msa1374140:intron 25.0%
! ACACTTGAAAAAATCAACAA+GGG + tig0019465:131454-131473 Msa1374140:intron 25.0%
!!! TATTATATATAGTGTTGGTG+GGG - tig0019465:131513-131532 None:intergenic 25.0%
!!! GTATTATATATAGTGTTGGT+GGG - tig0019465:131514-131533 None:intergenic 25.0%
!! AGTATTATATATAGTGTTGG+TGG - tig0019465:131515-131534 None:intergenic 25.0%
!! TAGTTTTCTAATATGCAAGA+AGG - tig0019465:131770-131789 None:intergenic 25.0%
!! TATATTTTCGGATTTCACTT+AGG - tig0019465:131819-131838 None:intergenic 25.0%
! TCAAGTACGTTCATATTAAA+GGG - tig0019465:132111-132130 None:intergenic 25.0%
! ACTATAATAATTGGAAGAGT+AGG - tig0019465:132179-132198 None:intergenic 25.0%
TCCATTTAGAAATTGCATTG+TGG + tig0019465:130712-130731 Msa1374140:five_prime_UTR 30.0%
ACCACAATGCAATTTCTAAA+TGG - tig0019465:130716-130735 None:intergenic 30.0%
! AGAAAAAATGGGTTCTCTTA+TGG + tig0019465:130761-130780 Msa1374140:exon 30.0%
TCTTCAATTGATCCTAAATC+AGG + tig0019465:130798-130817 Msa1374140:CDS 30.0%
AATCATATGGTACCTGATTT+AGG - tig0019465:130813-130832 None:intergenic 30.0%
! GTGCTTAGTATTTTATGTTG+CGG + tig0019465:130868-130887 Msa1374140:intron 30.0%
AGACGGTACTTATATTTATG+AGG + tig0019465:130915-130934 Msa1374140:intron 30.0%
GACGGTACTTATATTTATGA+GGG + tig0019465:130916-130935 Msa1374140:intron 30.0%
! TTTCATCTTTTGTAAGTGAC+TGG - tig0019465:130978-130997 None:intergenic 30.0%
GAAATCAAAGAAGAAGATAG+AGG + tig0019465:131011-131030 Msa1374140:CDS 30.0%
ACAAATTGGAAATTGCTGTA+AGG - tig0019465:131044-131063 None:intergenic 30.0%
CTGAATAGTCTTTGACAAAT+TGG - tig0019465:131058-131077 None:intergenic 30.0%
CAATTTGTCAAAGACTATTC+AGG + tig0019465:131056-131075 Msa1374140:CDS 30.0%
AATGAAATGTCAGAGAAAGT+TGG + tig0019465:131208-131227 Msa1374140:intron 30.0%
ACTCAAACACACAAGAAAAA+TGG - tig0019465:131391-131410 None:intergenic 30.0%
TTTCTTGTGTGTTTGAGTAA+TGG + tig0019465:131393-131412 Msa1374140:intron 30.0%
TTACTCTAGTAGAAGAATAC+TGG - tig0019465:131640-131659 None:intergenic 30.0%
ACGCGACAAACATTAATTAT+TGG + tig0019465:131671-131690 Msa1374140:intron 30.0%
AATATGCAAGAAGGACTAAA+AGG - tig0019465:131761-131780 None:intergenic 30.0%
CAAGTACGTTCATATTAAAG+GGG - tig0019465:132110-132129 None:intergenic 30.0%
GTCAAGTACGTTCATATTAA+AGG - tig0019465:132112-132131 None:intergenic 30.0%
GAGCAACTTATCAACAAAAA+TGG + tig0019465:132141-132160 Msa1374140:CDS 30.0%
AACTTATCAACAAAAATGGC+TGG + tig0019465:132145-132164 Msa1374140:CDS 30.0%
!! TAATTTAATTTGAGTGCAGG+TGG + tig0019465:132297-132316 Msa1374140:intron 30.0%
TCAATCACTAACTTCATCTA+CGG - tig0019465:132432-132451 None:intergenic 30.0%
TCCATTTAGAAATTGCATTG+TGG + tig0019465:130712-130731 Msa1374140:five_prime_UTR 30.0%
ACCACAATGCAATTTCTAAA+TGG - tig0019465:130716-130735 None:intergenic 30.0%
! AGAAAAAATGGGTTCTCTTA+TGG + tig0019465:130761-130780 Msa1374140:exon 30.0%
TCTTCAATTGATCCTAAATC+AGG + tig0019465:130798-130817 Msa1374140:CDS 30.0%
AATCATATGGTACCTGATTT+AGG - tig0019465:130813-130832 None:intergenic 30.0%
! GTGCTTAGTATTTTATGTTG+CGG + tig0019465:130868-130887 Msa1374140:intron 30.0%
AGACGGTACTTATATTTATG+AGG + tig0019465:130915-130934 Msa1374140:intron 30.0%
GACGGTACTTATATTTATGA+GGG + tig0019465:130916-130935 Msa1374140:intron 30.0%
! TTTCATCTTTTGTAAGTGAC+TGG - tig0019465:130978-130997 None:intergenic 30.0%
GAAATCAAAGAAGAAGATAG+AGG + tig0019465:131011-131030 Msa1374140:CDS 30.0%
ACAAATTGGAAATTGCTGTA+AGG - tig0019465:131044-131063 None:intergenic 30.0%
CTGAATAGTCTTTGACAAAT+TGG - tig0019465:131058-131077 None:intergenic 30.0%
CAATTTGTCAAAGACTATTC+AGG + tig0019465:131056-131075 Msa1374140:CDS 30.0%
AATGAAATGTCAGAGAAAGT+TGG + tig0019465:131208-131227 Msa1374140:intron 30.0%
ACTCAAACACACAAGAAAAA+TGG - tig0019465:131391-131410 None:intergenic 30.0%
TTTCTTGTGTGTTTGAGTAA+TGG + tig0019465:131393-131412 Msa1374140:intron 30.0%
TTACTCTAGTAGAAGAATAC+TGG - tig0019465:131640-131659 None:intergenic 30.0%
ACGCGACAAACATTAATTAT+TGG + tig0019465:131671-131690 Msa1374140:intron 30.0%
AATATGCAAGAAGGACTAAA+AGG - tig0019465:131761-131780 None:intergenic 30.0%
CAAGTACGTTCATATTAAAG+GGG - tig0019465:132110-132129 None:intergenic 30.0%
GTCAAGTACGTTCATATTAA+AGG - tig0019465:132112-132131 None:intergenic 30.0%
GAGCAACTTATCAACAAAAA+TGG + tig0019465:132141-132160 Msa1374140:CDS 30.0%
AACTTATCAACAAAAATGGC+TGG + tig0019465:132145-132164 Msa1374140:CDS 30.0%
!! TAATTTAATTTGAGTGCAGG+TGG + tig0019465:132297-132316 Msa1374140:intron 30.0%
TCAATCACTAACTTCATCTA+CGG - tig0019465:132432-132451 None:intergenic 30.0%
CTGTTTCAGCCATACTTAAA+AGG + tig0019465:130950-130969 Msa1374140:intron 35.0%
!! TGACTGGTTCCTTTTAAGTA+TGG - tig0019465:130962-130981 None:intergenic 35.0%
!! TTTGGAAAAATTTGGTGTGG+CGG - tig0019465:131151-131170 None:intergenic 35.0%
!! TGACTAAGAGCACTACAATT+TGG + tig0019465:131541-131560 Msa1374140:intron 35.0%
! ATTTTCGGATTTCACTTAGG+TGG - tig0019465:131816-131835 None:intergenic 35.0%
!! TGAAACTTCTTTTCAGGATC+AGG - tig0019465:132051-132070 None:intergenic 35.0%
!!! GACTTCTGAAACTTCTTTTC+AGG - tig0019465:132057-132076 None:intergenic 35.0%
GAACGTACTTGACACAAATT+TGG + tig0019465:132119-132138 Msa1374140:CDS 35.0%
AATCACTAACTTCATCTACG+GGG - tig0019465:132430-132449 None:intergenic 35.0%
CAATCACTAACTTCATCTAC+GGG - tig0019465:132431-132450 None:intergenic 35.0%
CTGTTTCAGCCATACTTAAA+AGG + tig0019465:130950-130969 Msa1374140:intron 35.0%
!! TGACTGGTTCCTTTTAAGTA+TGG - tig0019465:130962-130981 None:intergenic 35.0%
!! TTTGGAAAAATTTGGTGTGG+CGG - tig0019465:131151-131170 None:intergenic 35.0%
!! TGACTAAGAGCACTACAATT+TGG + tig0019465:131541-131560 Msa1374140:intron 35.0%
! ATTTTCGGATTTCACTTAGG+TGG - tig0019465:131816-131835 None:intergenic 35.0%
!! TGAAACTTCTTTTCAGGATC+AGG - tig0019465:132051-132070 None:intergenic 35.0%
!!! GACTTCTGAAACTTCTTTTC+AGG - tig0019465:132057-132076 None:intergenic 35.0%
GAACGTACTTGACACAAATT+TGG + tig0019465:132119-132138 Msa1374140:CDS 35.0%
AATCACTAACTTCATCTACG+GGG - tig0019465:132430-132449 None:intergenic 35.0%
CAATCACTAACTTCATCTAC+GGG - tig0019465:132431-132450 None:intergenic 35.0%
! AAAATGGGTTCTCTTATGGC+AGG + tig0019465:130765-130784 Msa1374140:exon 40.0%
!! ATCCAACTTATGGTTTGGTG+CGG + tig0019465:131122-131141 Msa1374140:intron 40.0%
AAAGAAGAAGCAGACAGAGT+TGG - tig0019465:131366-131385 None:intergenic 40.0%
ATGTTTGTCGCGTCTCAAAT+AGG - tig0019465:131664-131683 None:intergenic 40.0%
AAACTGTGCTGCATACTTGT+TGG - tig0019465:132375-132394 None:intergenic 40.0%
! CAATTTTGTCGATCCCAAAC+TGG - tig0019465:132405-132424 None:intergenic 40.0%
CCAAAAACGAACAACGCTAT+TGG - tig0019465:132537-132556 None:intergenic 40.0%
! CCAATAGCGTTGTTCGTTTT+TGG + tig0019465:132534-132553 Msa1374140:three_prime_UTR 40.0%
GCAAATCAACGTGTGTTTCT+TGG + tig0019465:132599-132618 Msa1374140:three_prime_UTR 40.0%
! AAAATGGGTTCTCTTATGGC+AGG + tig0019465:130765-130784 Msa1374140:exon 40.0%
!! ATCCAACTTATGGTTTGGTG+CGG + tig0019465:131122-131141 Msa1374140:intron 40.0%
AAAGAAGAAGCAGACAGAGT+TGG - tig0019465:131366-131385 None:intergenic 40.0%
ATGTTTGTCGCGTCTCAAAT+AGG - tig0019465:131664-131683 None:intergenic 40.0%
AAACTGTGCTGCATACTTGT+TGG - tig0019465:132375-132394 None:intergenic 40.0%
! CAATTTTGTCGATCCCAAAC+TGG - tig0019465:132405-132424 None:intergenic 40.0%
CCAAAAACGAACAACGCTAT+TGG - tig0019465:132537-132556 None:intergenic 40.0%
! CCAATAGCGTTGTTCGTTTT+TGG + tig0019465:132534-132553 Msa1374140:three_prime_UTR 40.0%
GCAAATCAACGTGTGTTTCT+TGG + tig0019465:132599-132618 Msa1374140:three_prime_UTR 40.0%
!! TACTTGCCACTTTCCAGCAT+GGG + tig0019465:131096-131115 Msa1374140:intron 45.0%
GGCAGATCCAACTTATGGTT+TGG + tig0019465:131117-131136 Msa1374140:intron 45.0%
! AAGAAGCAGACAGAGTTGGT+GGG - tig0019465:131362-131381 None:intergenic 45.0%
ATTAAAGGGGTCCCTTACGT+GGG - tig0019465:132097-132116 None:intergenic 45.0%
TATTAAAGGGGTCCCTTACG+TGG - tig0019465:132098-132117 None:intergenic 45.0%
!! CAGGTGGACTAAGAGTAACT+GGG + tig0019465:132313-132332 Msa1374140:intron 45.0%
TCTGAATGAGCCACCAATGA+TGG + tig0019465:132340-132359 Msa1374140:CDS 45.0%
TTGGATGCAGCTTCCATCAT+TGG - tig0019465:132356-132375 None:intergenic 45.0%
ACAGTTTCACGTTGCCAGTT+TGG + tig0019465:132388-132407 Msa1374140:CDS 45.0%
CAGTTTCACGTTGCCAGTTT+GGG + tig0019465:132389-132408 Msa1374140:CDS 45.0%
!! TACTTGCCACTTTCCAGCAT+GGG + tig0019465:131096-131115 Msa1374140:intron 45.0%
GGCAGATCCAACTTATGGTT+TGG + tig0019465:131117-131136 Msa1374140:intron 45.0%
! AAGAAGCAGACAGAGTTGGT+GGG - tig0019465:131362-131381 None:intergenic 45.0%
ATTAAAGGGGTCCCTTACGT+GGG - tig0019465:132097-132116 None:intergenic 45.0%
TATTAAAGGGGTCCCTTACG+TGG - tig0019465:132098-132117 None:intergenic 45.0%
!! CAGGTGGACTAAGAGTAACT+GGG + tig0019465:132313-132332 Msa1374140:intron 45.0%
TCTGAATGAGCCACCAATGA+TGG + tig0019465:132340-132359 Msa1374140:CDS 45.0%
TTGGATGCAGCTTCCATCAT+TGG - tig0019465:132356-132375 None:intergenic 45.0%
ACAGTTTCACGTTGCCAGTT+TGG + tig0019465:132388-132407 Msa1374140:CDS 45.0%
CAGTTTCACGTTGCCAGTTT+GGG + tig0019465:132389-132408 Msa1374140:CDS 45.0%
!!! TGTATTTTATTTATTTTTTT+TGG - tig0019465:131169-131188 None:intergenic 5.0%
!! TATTATATAACTATAATAAT+TGG - tig0019465:132188-132207 None:intergenic 5.0%
!! TATTATAGTTATATAATAAT+TGG + tig0019465:132190-132209 Msa1374140:intron 5.0%
!!! TGTATTTTATTTATTTTTTT+TGG - tig0019465:131169-131188 None:intergenic 5.0%
!! TATTATATAACTATAATAAT+TGG - tig0019465:132188-132207 None:intergenic 5.0%
!! TATTATAGTTATATAATAAT+TGG + tig0019465:132190-132209 Msa1374140:intron 5.0%
TGGGTTCTCTTATGGCAGGA+TGG + tig0019465:130769-130788 Msa1374140:CDS 50.0%
GGGTTCTCTTATGGCAGGAT+GGG + tig0019465:130770-130789 Msa1374140:CDS 50.0%
GGTTCTCTTATGGCAGGATG+GGG + tig0019465:130771-130790 Msa1374140:CDS 50.0%
TGCTGGAAAGTGGCAAGTAG+AGG - tig0019465:131095-131114 None:intergenic 50.0%
!! CTACTTGCCACTTTCCAGCA+TGG + tig0019465:131095-131114 Msa1374140:intron 50.0%
! TAAGTTGGATCTGCCCATGC+TGG - tig0019465:131112-131131 None:intergenic 50.0%
GCATGGGCAGATCCAACTTA+TGG + tig0019465:131112-131131 Msa1374140:intron 50.0%
AGCCGCACCAAACCATAAGT+TGG - tig0019465:131127-131146 None:intergenic 50.0%
!! AGAAGCAGACAGAGTTGGTG+GGG - tig0019465:131361-131380 None:intergenic 50.0%
GAAGAAGCAGACAGAGTTGG+TGG - tig0019465:131363-131382 None:intergenic 50.0%
!! GCAGGTGGACTAAGAGTAAC+TGG + tig0019465:132312-132331 Msa1374140:intron 50.0%
GATGCAGCTTCCATCATTGG+TGG - tig0019465:132353-132372 None:intergenic 50.0%
TGGGTTCTCTTATGGCAGGA+TGG + tig0019465:130769-130788 Msa1374140:CDS 50.0%
GGGTTCTCTTATGGCAGGAT+GGG + tig0019465:130770-130789 Msa1374140:CDS 50.0%
GGTTCTCTTATGGCAGGATG+GGG + tig0019465:130771-130790 Msa1374140:CDS 50.0%
TGCTGGAAAGTGGCAAGTAG+AGG - tig0019465:131095-131114 None:intergenic 50.0%
!! CTACTTGCCACTTTCCAGCA+TGG + tig0019465:131095-131114 Msa1374140:intron 50.0%
! TAAGTTGGATCTGCCCATGC+TGG - tig0019465:131112-131131 None:intergenic 50.0%
GCATGGGCAGATCCAACTTA+TGG + tig0019465:131112-131131 Msa1374140:intron 50.0%
AGCCGCACCAAACCATAAGT+TGG - tig0019465:131127-131146 None:intergenic 50.0%
!! AGAAGCAGACAGAGTTGGTG+GGG - tig0019465:131361-131380 None:intergenic 50.0%
GAAGAAGCAGACAGAGTTGG+TGG - tig0019465:131363-131382 None:intergenic 50.0%
!! GCAGGTGGACTAAGAGTAAC+TGG + tig0019465:132312-132331 Msa1374140:intron 50.0%
GATGCAGCTTCCATCATTGG+TGG - tig0019465:132353-132372 None:intergenic 50.0%
GATCTGCCCATGCTGGAAAG+TGG - tig0019465:131105-131124 None:intergenic 55.0%
GAAGTCAATGACCATGCCCG+TGG + tig0019465:132071-132090 Msa1374140:CDS 55.0%
GATCTGCCCATGCTGGAAAG+TGG - tig0019465:131105-131124 None:intergenic 55.0%
GAAGTCAATGACCATGCCCG+TGG + tig0019465:132071-132090 Msa1374140:CDS 55.0%
CATGCCCGTGGCCCACGTAA+GGG + tig0019465:132083-132102 Msa1374140:CDS 65.0%
CATGCCCGTGGCCCACGTAA+GGG + tig0019465:132083-132102 Msa1374140:CDS 65.0%
CCTTACGTGGGCCACGGGCA+TGG - tig0019465:132085-132104 None:intergenic 70.0%
CCATGCCCGTGGCCCACGTA+AGG + tig0019465:132082-132101 Msa1374140:CDS 70.0%
GGGTCCCTTACGTGGGCCAC+GGG - tig0019465:132090-132109 None:intergenic 70.0%
GGGGTCCCTTACGTGGGCCA+CGG - tig0019465:132091-132110 None:intergenic 70.0%
CCTTACGTGGGCCACGGGCA+TGG - tig0019465:132085-132104 None:intergenic 70.0%
CCATGCCCGTGGCCCACGTA+AGG + tig0019465:132082-132101 Msa1374140:CDS 70.0%
GGGTCCCTTACGTGGGCCAC+GGG - tig0019465:132090-132109 None:intergenic 70.0%
GGGGTCCCTTACGTGGGCCA+CGG - tig0019465:132091-132110 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0019465 gene 130704 132664 130704 ID=Msa1374140;Name=Msa1374140
tig0019465 mRNA 130704 132664 130704 ID=Msa1374140-mRNA-1;Parent=Msa1374140;Name=Msa1374140-mRNA-1;_AED=0.07;_eAED=0.07;_QI=64|1|1|1|1|1|4|213|141
tig0019465 exon 130704 130819 130704 ID=Msa1374140-mRNA-1:exon:3681;Parent=Msa1374140-mRNA-1
tig0019465 exon 130959 131077 130959 ID=Msa1374140-mRNA-1:exon:3682;Parent=Msa1374140-mRNA-1
tig0019465 exon 132048 132166 132048 ID=Msa1374140-mRNA-1:exon:3683;Parent=Msa1374140-mRNA-1
tig0019465 exon 132316 132664 132316 ID=Msa1374140-mRNA-1:exon:3684;Parent=Msa1374140-mRNA-1
tig0019465 five_prime_UTR 130704 130767 130704 ID=Msa1374140-mRNA-1:five_prime_utr;Parent=Msa1374140-mRNA-1
tig0019465 CDS 130768 130819 130768 ID=Msa1374140-mRNA-1:cds;Parent=Msa1374140-mRNA-1
tig0019465 CDS 130959 131077 130959 ID=Msa1374140-mRNA-1:cds;Parent=Msa1374140-mRNA-1
tig0019465 CDS 132048 132166 132048 ID=Msa1374140-mRNA-1:cds;Parent=Msa1374140-mRNA-1
tig0019465 CDS 132316 132451 132316 ID=Msa1374140-mRNA-1:cds;Parent=Msa1374140-mRNA-1
tig0019465 three_prime_UTR 132452 132664 132452 ID=Msa1374140-mRNA-1:three_prime_utr;Parent=Msa1374140-mRNA-1
Gene Sequence

>Msa1374140

ATGGGTTCTCTTATGGCAGGATGGGGCTCTTCTTCAATTGATCCTAAATCAGCCATACTTAAAAGGAACCAGTCACTTACAAAAGATGAAATTGATGCTTATTGGAAATCAAAGAAGAAGATAGAGGAAGAACACCTTACAGCAATTTCCAATTTGTCAAAGACTATTCAGCCTGATCCTGAAAAGAAGTTTCAGAAGTCAATGACCATGCCCGTGGCCCACGTAAGGGACCCCTTTAATATGAACGTACTTGACACAAATTTGGAGCAACTTATCAACAAAAATGGCTGGTGGACTAAGAGTAACTGGGCATTTCTGAATGAGCCACCAATGATGGAAGCTGCATCCAACAAGTATGCAGCACAGTTTCACGTTGCCAGTTTGGGATCGACAAAATTGAACCCCGTAGATGAAGTTAGTGATTGA
Protein sequence

>Msa1374140

MGSLMAGWGSSSIDPKSAILKRNQSLTKDEIDAYWKSKKKIEEEHLTAISNLSKTIQPDPEKKFQKSMTMPVAHVRDPFNMNVLDTNLEQLINKNGWWTKSNWAFLNEPPMMEAASNKYAAQFHVASLGSTKLNPVDEVSD*