AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1377170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1377170 MtrunA17_Chr2g0323051 87.500 72 9 0 1 72 1 72 1.43e-37 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGCTTTGTTCTTGAATT+TGG 0.098697 tig0019874:+55327 None:intergenic
CAGTGTTGGTTCTTCATTCA+TGG 0.286766 tig0019874:+55624 None:intergenic
CATGAAGAAACGAGTGAAAA+AGG 0.295336 tig0019874:-55699 None:intergenic
TATATGCATTGAAGTGCTTA+AGG 0.345801 tig0019874:-55484 Msa1377170:five_prime_UTR
ATGAATATTATCAAATTTGA+AGG 0.373538 tig0019874:+55305 None:intergenic
CCTTATTTATCTATGATCAT+TGG 0.375895 tig0019874:-55648 Msa1377170:five_prime_UTR
TTCGTATAATTTGCAACATT+AGG 0.381270 tig0019874:-55461 Msa1377170:five_prime_UTR
CCAATGATCATAGATAAATA+AGG 0.417804 tig0019874:+55648 None:intergenic
GATAAAATGGTTACCTCTGC+AGG 0.461646 tig0019874:+55216 None:intergenic
TTGTTGTGTTTGTGTAATGA+AGG 0.462695 tig0019874:+55536 None:intergenic
TTTATGTGTTTCCTAAAACT+TGG 0.489544 tig0019874:-55588 Msa1377170:five_prime_UTR
ATTTGCAACATTAGGAGGAA+AGG 0.504203 tig0019874:-55453 Msa1377170:five_prime_UTR
GTTAATTAGTGTATAAAATG+AGG 0.528418 tig0019874:-55431 Msa1377170:exon
TGTTGTGTTTGTGTAATGAA+GGG 0.535913 tig0019874:+55537 None:intergenic
GTATAATTTGCAACATTAGG+AGG 0.545524 tig0019874:-55458 Msa1377170:five_prime_UTR
AAACACATAAATAAGAGAAG+TGG 0.573088 tig0019874:+55600 None:intergenic
ATAAGAGAAGTGGACAGTGT+TGG 0.612446 tig0019874:+55610 None:intergenic
AGCAATTGCTGAACCTGCAG+AGG 0.618481 tig0019874:-55229 Msa1377170:intron
CTATACGTGCAGATAGAGCA+AGG 0.627427 tig0019874:-55353 Msa1377170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTATTTTTATAATATACA+TGG + tig0019874:55224-55243 None:intergenic 10.0%
!! ATGAATATTATCAAATTTGA+AGG + tig0019874:55483-55502 None:intergenic 15.0%
!!! TATAATTATTTGCTACTATA+TGG + tig0019874:55607-55626 None:intergenic 15.0%
!! GTTAATTAGTGTATAAAATG+AGG - tig0019874:55354-55373 Msa1377170:CDS 20.0%
!! GAAAGGAAAATATGATAAAA+TGG + tig0019874:55585-55604 None:intergenic 20.0%
! CCAATGATCATAGATAAATA+AGG + tig0019874:55140-55159 None:intergenic 25.0%
!! CCTTATTTATCTATGATCAT+TGG - tig0019874:55137-55156 Msa1377170:intron 25.0%
! AAACACATAAATAAGAGAAG+TGG + tig0019874:55188-55207 None:intergenic 25.0%
! TTTATGTGTTTCCTAAAACT+TGG - tig0019874:55197-55216 Msa1377170:intron 25.0%
!! TTCGTATAATTTGCAACATT+AGG - tig0019874:55324-55343 Msa1377170:CDS 25.0%
!! TTATTTGCTACTATATGGAA+AGG + tig0019874:55602-55621 None:intergenic 25.0%
! ATATACATGGACCAAGTTTT+AGG + tig0019874:55211-55230 None:intergenic 30.0%
TGTTGTGTTTGTGTAATGAA+GGG + tig0019874:55251-55270 None:intergenic 30.0%
TTGTTGTGTTTGTGTAATGA+AGG + tig0019874:55252-55271 None:intergenic 30.0%
! TATATGCATTGAAGTGCTTA+AGG - tig0019874:55301-55320 Msa1377170:CDS 30.0%
! GTATAATTTGCAACATTAGG+AGG - tig0019874:55327-55346 Msa1377170:CDS 30.0%
!!! TTCACGAAAGGTATTTTCAA+AGG - tig0019874:55399-55418 Msa1377170:CDS 30.0%
ATTTGCAACATTAGGAGGAA+AGG - tig0019874:55332-55351 Msa1377170:CDS 35.0%
! GACGACGATTAATACTTTTG+AGG - tig0019874:55376-55395 Msa1377170:CDS 35.0%
!!! ATACTTTTGAGGTTCACGAA+AGG - tig0019874:55387-55406 Msa1377170:CDS 35.0%
! GCTTGCTTTGTTCTTGAATT+TGG + tig0019874:55461-55480 None:intergenic 35.0%
! AAAAAGGACACACTGACTGA+CGG - tig0019874:55102-55121 Msa1377170:CDS 40.0%
!! CAGTGTTGGTTCTTCATTCA+TGG + tig0019874:55164-55183 None:intergenic 40.0%
ATAAGAGAAGTGGACAGTGT+TGG + tig0019874:55178-55197 None:intergenic 40.0%
GATAAAATGGTTACCTCTGC+AGG + tig0019874:55572-55591 None:intergenic 40.0%
CTATACGTGCAGATAGAGCA+AGG - tig0019874:55432-55451 Msa1377170:exon 45.0%
AGCAATTGCTGAACCTGCAG+AGG - tig0019874:55556-55575 Msa1377170:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
tig0019874 gene 55100 55707 55100 ID=Msa1377170;Name=Msa1377170
tig0019874 mRNA 55100 55707 55100 ID=Msa1377170-mRNA-1;Parent=Msa1377170;Name=Msa1377170-mRNA-1;_AED=0.46;_eAED=0.46;_QI=271|0|0.5|0.5|1|1|2|0|79
tig0019874 exon 55100 55132 55100 ID=Msa1377170-mRNA-1:exon:5553;Parent=Msa1377170-mRNA-1
tig0019874 exon 55230 55707 55230 ID=Msa1377170-mRNA-1:exon:5552;Parent=Msa1377170-mRNA-1
tig0019874 five_prime_UTR 55437 55707 55437 ID=Msa1377170-mRNA-1:five_prime_utr;Parent=Msa1377170-mRNA-1
tig0019874 CDS 55230 55436 55230 ID=Msa1377170-mRNA-1:cds;Parent=Msa1377170-mRNA-1
tig0019874 CDS 55100 55132 55100 ID=Msa1377170-mRNA-1:cds;Parent=Msa1377170-mRNA-1
Gene Sequence

>Msa1377170

ATGAGGACGACGATTAATACTTTTGAGGTTCACGAAAGGTATTTTCAAAGGAGTAACGAAGCTATACGTGCAGATAGAGCAAGGAAGCCAAATTCAAGAACAAAGCAAGCCTTCAAATTTGATAATATTCATCGTGCTTCTGTCAACAAACAACGTTCTGTGTCTTTGTCAGAATTCAAAGCTACAGCAATTGCTGAACCTGCAGAGATGCATATTCACGCATTGTTGAACTCATTTTAG

Protein sequence

>Msa1377170

MRTTINTFEVHERYFQRSNEAIRADRARKPNSRTKQAFKFDNIHRASVNKQRSVSLSEFKATAIAEPAEMHIHALLNSF*