AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0374870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374870 MtrunA17_Chr3g0125561 94.175 103 2 2 1 102 31 130 1.11e-63 197
Msa0374870 MtrunA17_Chr1g0159571 42.105 152 74 5 1 145 1 145 1.71e-24 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374870 AT1G21390.1 46.591 88 43 2 59 146 52 135 3.70e-14 68.2
Msa0374870 AT1G76980.1 50.000 72 27 2 58 127 49 113 7.31e-14 67.4
Msa0374870 AT1G76980.2 50.000 72 27 2 58 127 49 113 1.04e-13 67.0

Find 55 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATCATCATGTTTAA+TGG 0.197990 3_1:-74398947 Msa0374870:three_prime_UTR
GAATTAGATAACTTTGCTTC+TGG 0.283440 3_1:-74399003 Msa0374870:three_prime_UTR
ATGGCCTCTGTTCTTGAAAC+AGG 0.336109 3_1:+74400228 None:intergenic
TACAAATCAAAAGAATTCTT+AGG 0.338959 3_1:+74400387 None:intergenic
GAAAGTTCATAGTTTGATTC+GGG 0.347032 3_1:+74400162 None:intergenic
CCAGAGATTATTAGAATTTG+TGG 0.349920 3_1:+74400335 None:intergenic
TGAAAGTTCATAGTTTGATT+CGG 0.368860 3_1:+74400161 None:intergenic
TTCTTTATATGTTTATTTGA+AGG 0.375267 3_1:-74398871 None:intergenic
GCCTTCAACACAGCCTCAAT+TGG 0.391567 3_1:-74400313 Msa0374870:CDS
AGGAGGAGGAGGAGAGGAGA+AGG 0.392151 3_1:-74400080 Msa0374870:CDS
GGCTTCCCATGTTTCATGTC+TGG 0.393616 3_1:+74400356 None:intergenic
AAACTATGAACTTTCATTGA+AGG 0.394782 3_1:-74400154 Msa0374870:CDS
AAGAATTCTAGTGTCTATAA+TGG 0.423589 3_1:-74400046 Msa0374870:CDS
AGCAGAAGGAGGAGGAGGAG+AGG 0.427193 3_1:-74400086 Msa0374870:CDS
ATCACGATGAACAACCTAGA+TGG 0.455248 3_1:-74400284 Msa0374870:CDS
AGCAAAGTTATCTAATTCAA+TGG 0.455720 3_1:+74399008 None:intergenic
ATATCATCATGTTTAATGGC+AGG 0.462367 3_1:-74398943 Msa0374870:three_prime_UTR
CCACAAATTCTAATAATCTC+TGG 0.468437 3_1:-74400335 Msa0374870:CDS
AAGAACTCATGGAAATGGTT+CGG 0.483414 3_1:-74400191 Msa0374870:CDS
AGAAAATACAGAGGAGCAGA+AGG 0.490882 3_1:-74400100 Msa0374870:CDS
TCATGTTTAATGGCAGGGCA+TGG 0.506028 3_1:-74398937 Msa0374870:three_prime_UTR
AGGAGGAGAGGAGAAGGAGA+AGG 0.506195 3_1:-74400074 Msa0374870:CDS
AGAGGAGCAGAAGGAGGAGG+AGG 0.512087 3_1:-74400091 Msa0374870:CDS
TCCAATTGAGGCTGTGTTGA+AGG 0.516902 3_1:+74400312 None:intergenic
GGTGAGTTCACACTCCATCT+AGG 0.517049 3_1:+74400270 None:intergenic
ATCTTGTGGAACACCACCAC+AGG 0.517846 3_1:-74400131 Msa0374870:CDS
ATAGTACTTTGTTTCATCAA+AGG 0.520728 3_1:+74399150 None:intergenic
ACTTTCATTGAAGGATCTTG+TGG 0.526030 3_1:-74400145 Msa0374870:CDS
CTTTGTTTCATCAAAGGCAT+CGG 0.528423 3_1:+74399156 None:intergenic
TTACAAAGTTTGGATTGGCA+TGG 0.529260 3_1:+74398908 None:intergenic
TACAGAGGAGCAGAAGGAGG+AGG 0.532433 3_1:-74400094 Msa0374870:CDS
AATCAAAAGAATTCTTAGGT+TGG 0.534469 3_1:+74400391 None:intergenic
CAAAAGAATTCTTAGGTTGG+TGG 0.537203 3_1:+74400394 None:intergenic
TTTCTTCTCTGGTCCTGTGG+TGG 0.548885 3_1:+74400118 None:intergenic
CATGTTTAATGGCAGGGCAT+GGG 0.551016 3_1:-74398936 Msa0374870:three_prime_UTR
CAAGAAGATTGATAATAAGA+AGG 0.553116 3_1:-74400020 Msa0374870:intron
CTGTTCTTGAAACAGGGGAA+AGG 0.560820 3_1:+74400235 None:intergenic
AGAAGGGTGGAGGTATATGC+TGG 0.561488 3_1:-74399225 Msa0374870:CDS
TGCAGGCGTGAGAAGGGTGG+AGG 0.562031 3_1:-74399235 Msa0374870:intron
TCGTGATACATGTCCAATTG+AGG 0.569340 3_1:+74400300 None:intergenic
GTTTAATGGCAGGGCATGGG+AGG 0.571187 3_1:-74398933 Msa0374870:three_prime_UTR
TATCATCATGTTTAATGGCA+GGG 0.575049 3_1:-74398942 Msa0374870:three_prime_UTR
TGGCCTCTGTTCTTGAAACA+GGG 0.597218 3_1:+74400229 None:intergenic
ATGAAACCAGACATGAAACA+TGG 0.602607 3_1:-74400362 Msa0374870:CDS
AGAACAGAGGCCATAGTGAG+AGG 0.606015 3_1:-74400219 Msa0374870:CDS
AAATGCAGGCGTGAGAAGGG+TGG 0.606501 3_1:-74399238 Msa0374870:intron
AATTCTAGTGTCTATAATGG+TGG 0.616757 3_1:-74400043 Msa0374870:CDS
TTCCCCTGTTTCAAGAACAG+AGG 0.619257 3_1:-74400232 Msa0374870:CDS
GGGGAAAGGCTTCTGTAATG+TGG 0.623224 3_1:+74400249 None:intergenic
AAATACAGAGGAGCAGAAGG+AGG 0.627616 3_1:-74400097 Msa0374870:CDS
GATGAAATGCAGGCGTGAGA+AGG 0.636676 3_1:-74399242 Msa0374870:intron
ATGAAATGCAGGCGTGAGAA+GGG 0.643514 3_1:-74399241 Msa0374870:intron
GGCCTCTGTTCTTGAAACAG+GGG 0.643547 3_1:+74400230 None:intergenic
TGAAACCAGACATGAAACAT+GGG 0.668487 3_1:-74400361 Msa0374870:CDS
GACCAGAGAAGAAAATACAG+AGG 0.687498 3_1:-74400109 Msa0374870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTAATTAATTTGAAGTT+GGG + chr3_1:74399643-74399662 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG + chr3_1:74399644-74399663 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG - chr3_1:74399773-74399792 Msa0374870:intron 15.0%
!!! AAGAAAATAACTCTTTTTAT+TGG - chr3_1:74399956-74399975 Msa0374870:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG + chr3_1:74400106-74400125 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG + chr3_1:74400107-74400126 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG + chr3_1:74400114-74400133 None:intergenic 15.0%
!!! ATGTTAATTAATTTGAAGTT+GGG + chr3_1:74399643-74399662 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG + chr3_1:74399644-74399663 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG - chr3_1:74399773-74399792 Msa0374870:intron 15.0%
!!! AAGAAAATAACTCTTTTTAT+TGG - chr3_1:74399956-74399975 Msa0374870:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG + chr3_1:74400106-74400125 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG + chr3_1:74400107-74400126 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG + chr3_1:74400114-74400133 None:intergenic 15.0%
!! TACAAATCAAAAGAATTCTT+AGG + chr3_1:74398906-74398925 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG + chr3_1:74399361-74399380 None:intergenic 20.0%
!! GTAAAAAAATTTCATGTTCA+TGG - chr3_1:74399840-74399859 Msa0374870:intron 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG - chr3_1:74399999-74400018 Msa0374870:intron 20.0%
!! AAAAAAAAATTGTTCTCAGA+TGG + chr3_1:74400226-74400245 None:intergenic 20.0%
!! ACAAATATCATCATGTTTAA+TGG - chr3_1:74400343-74400362 Msa0374870:CDS 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG + chr3_1:74400390-74400409 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG + chr3_1:74400395-74400414 None:intergenic 20.0%
!! TACAAATCAAAAGAATTCTT+AGG + chr3_1:74398906-74398925 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG + chr3_1:74399361-74399380 None:intergenic 20.0%
!! GTAAAAAAATTTCATGTTCA+TGG - chr3_1:74399840-74399859 Msa0374870:intron 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG - chr3_1:74399999-74400018 Msa0374870:intron 20.0%
!! AAAAAAAAATTGTTCTCAGA+TGG + chr3_1:74400226-74400245 None:intergenic 20.0%
!! ACAAATATCATCATGTTTAA+TGG - chr3_1:74400343-74400362 Msa0374870:CDS 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG + chr3_1:74400390-74400409 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG + chr3_1:74400395-74400414 None:intergenic 20.0%
! AATCAAAAGAATTCTTAGGT+TGG + chr3_1:74398902-74398921 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG + chr3_1:74399132-74399151 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG - chr3_1:74399136-74399155 Msa0374870:three_prime_UTR 25.0%
! AAGAATTCTAGTGTCTATAA+TGG - chr3_1:74399244-74399263 Msa0374870:intron 25.0%
!! CAAGAAGATTGATAATAAGA+AGG - chr3_1:74399270-74399289 Msa0374870:intron 25.0%
! TACATGTGAAGAAAAATGAT+GGG - chr3_1:74399293-74399312 Msa0374870:intron 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG - chr3_1:74399381-74399400 Msa0374870:intron 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG - chr3_1:74399666-74399685 Msa0374870:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG - chr3_1:74399874-74399893 Msa0374870:intron 25.0%
! TAAGCAAAAAGACATGTATT+TGG + chr3_1:74399993-74400012 None:intergenic 25.0%
!!! ATAGAGTACTGATTAACTTT+TGG - chr3_1:74400026-74400045 Msa0374870:CDS 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG + chr3_1:74400143-74400162 None:intergenic 25.0%
! AGCAAAGTTATCTAATTCAA+TGG + chr3_1:74400285-74400304 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG - chr3_1:74400309-74400328 Msa0374870:CDS 25.0%
! AATCAAAAGAATTCTTAGGT+TGG + chr3_1:74398902-74398921 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG + chr3_1:74399132-74399151 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG - chr3_1:74399136-74399155 Msa0374870:three_prime_UTR 25.0%
! AAGAATTCTAGTGTCTATAA+TGG - chr3_1:74399244-74399263 Msa0374870:intron 25.0%
!! CAAGAAGATTGATAATAAGA+AGG - chr3_1:74399270-74399289 Msa0374870:intron 25.0%
! TACATGTGAAGAAAAATGAT+GGG - chr3_1:74399293-74399312 Msa0374870:intron 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG - chr3_1:74399381-74399400 Msa0374870:intron 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG - chr3_1:74399666-74399685 Msa0374870:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG - chr3_1:74399874-74399893 Msa0374870:intron 25.0%
! TAAGCAAAAAGACATGTATT+TGG + chr3_1:74399993-74400012 None:intergenic 25.0%
!!! ATAGAGTACTGATTAACTTT+TGG - chr3_1:74400026-74400045 Msa0374870:CDS 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG + chr3_1:74400143-74400162 None:intergenic 25.0%
! AGCAAAGTTATCTAATTCAA+TGG + chr3_1:74400285-74400304 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG - chr3_1:74400309-74400328 Msa0374870:CDS 25.0%
CCAGAGATTATTAGAATTTG+TGG + chr3_1:74398958-74398977 None:intergenic 30.0%
CCACAAATTCTAATAATCTC+TGG - chr3_1:74398955-74398974 Msa0374870:three_prime_UTR 30.0%
ACAAAAAGAACTCATGGAAA+TGG - chr3_1:74399094-74399113 Msa0374870:three_prime_UTR 30.0%
GAAAGTTCATAGTTTGATTC+GGG + chr3_1:74399131-74399150 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG - chr3_1:74399247-74399266 Msa0374870:intron 30.0%
GTACATGTGAAGAAAAATGA+TGG - chr3_1:74399292-74399311 Msa0374870:intron 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG - chr3_1:74399352-74399371 Msa0374870:intron 30.0%
TCACTCTCTTTATAAACAGA+AGG + chr3_1:74399493-74399512 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG + chr3_1:74399705-74399724 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG - chr3_1:74399847-74399866 Msa0374870:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG + chr3_1:74399935-74399954 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG + chr3_1:74399936-74399955 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG - chr3_1:74400038-74400057 Msa0374870:CDS 30.0%
GAATTAGATAACTTTGCTTC+TGG - chr3_1:74400287-74400306 Msa0374870:CDS 30.0%
ATATCATCATGTTTAATGGC+AGG - chr3_1:74400347-74400366 Msa0374870:CDS 30.0%
TATCATCATGTTTAATGGCA+GGG - chr3_1:74400348-74400367 Msa0374870:CDS 30.0%
CCAGAGATTATTAGAATTTG+TGG + chr3_1:74398958-74398977 None:intergenic 30.0%
CCACAAATTCTAATAATCTC+TGG - chr3_1:74398955-74398974 Msa0374870:three_prime_UTR 30.0%
ACAAAAAGAACTCATGGAAA+TGG - chr3_1:74399094-74399113 Msa0374870:three_prime_UTR 30.0%
GAAAGTTCATAGTTTGATTC+GGG + chr3_1:74399131-74399150 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG - chr3_1:74399247-74399266 Msa0374870:intron 30.0%
GTACATGTGAAGAAAAATGA+TGG - chr3_1:74399292-74399311 Msa0374870:intron 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG - chr3_1:74399352-74399371 Msa0374870:intron 30.0%
TCACTCTCTTTATAAACAGA+AGG + chr3_1:74399493-74399512 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG + chr3_1:74399705-74399724 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG - chr3_1:74399847-74399866 Msa0374870:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG + chr3_1:74399935-74399954 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG + chr3_1:74399936-74399955 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG - chr3_1:74400038-74400057 Msa0374870:CDS 30.0%
GAATTAGATAACTTTGCTTC+TGG - chr3_1:74400287-74400306 Msa0374870:CDS 30.0%
ATATCATCATGTTTAATGGC+AGG - chr3_1:74400347-74400366 Msa0374870:CDS 30.0%
TATCATCATGTTTAATGGCA+GGG - chr3_1:74400348-74400367 Msa0374870:CDS 30.0%
!! CAAAAGAATTCTTAGGTTGG+TGG + chr3_1:74398899-74398918 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG - chr3_1:74398928-74398947 Msa0374870:three_prime_UTR 35.0%
TGAAACCAGACATGAAACAT+GGG - chr3_1:74398929-74398948 Msa0374870:three_prime_UTR 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG + chr3_1:74399084-74399103 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG - chr3_1:74399099-74399118 Msa0374870:three_prime_UTR 35.0%
ACTTTCATTGAAGGATCTTG+TGG - chr3_1:74399145-74399164 Msa0374870:three_prime_UTR 35.0%
! ATTTCTGATGGACCAGTAAA+AGG - chr3_1:74399430-74399449 Msa0374870:intron 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG - chr3_1:74399674-74399693 Msa0374870:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG - chr3_1:74399898-74399917 Msa0374870:intron 35.0%
AATCAAATCCCATGTTGGAT+TGG - chr3_1:74399903-74399922 Msa0374870:intron 35.0%
ATCAAATCCCATGTTGGATT+GGG - chr3_1:74399904-74399923 Msa0374870:intron 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG + chr3_1:74400091-74400110 None:intergenic 35.0%
! CTTTGTTTCATCAAAGGCAT+CGG + chr3_1:74400137-74400156 None:intergenic 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG + chr3_1:74400385-74400404 None:intergenic 35.0%
!! CAAAAGAATTCTTAGGTTGG+TGG + chr3_1:74398899-74398918 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG - chr3_1:74398928-74398947 Msa0374870:three_prime_UTR 35.0%
TGAAACCAGACATGAAACAT+GGG - chr3_1:74398929-74398948 Msa0374870:three_prime_UTR 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG + chr3_1:74399084-74399103 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG - chr3_1:74399099-74399118 Msa0374870:three_prime_UTR 35.0%
ACTTTCATTGAAGGATCTTG+TGG - chr3_1:74399145-74399164 Msa0374870:three_prime_UTR 35.0%
! ATTTCTGATGGACCAGTAAA+AGG - chr3_1:74399430-74399449 Msa0374870:intron 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG - chr3_1:74399674-74399693 Msa0374870:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG - chr3_1:74399898-74399917 Msa0374870:intron 35.0%
AATCAAATCCCATGTTGGAT+TGG - chr3_1:74399903-74399922 Msa0374870:intron 35.0%
ATCAAATCCCATGTTGGATT+GGG - chr3_1:74399904-74399923 Msa0374870:intron 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG + chr3_1:74400091-74400110 None:intergenic 35.0%
! CTTTGTTTCATCAAAGGCAT+CGG + chr3_1:74400137-74400156 None:intergenic 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG + chr3_1:74400385-74400404 None:intergenic 35.0%
TCGTGATACATGTCCAATTG+AGG + chr3_1:74398993-74399012 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG - chr3_1:74399006-74399025 Msa0374870:three_prime_UTR 40.0%
GAGAGGACAAAAAGAACTCA+TGG - chr3_1:74399088-74399107 Msa0374870:three_prime_UTR 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG + chr3_1:74399178-74399197 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG - chr3_1:74399181-74399200 Msa0374870:exon 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG + chr3_1:74399186-74399205 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG - chr3_1:74399190-74399209 Msa0374870:CDS 40.0%
AAAAAGGTAACCCCACTTTC+AGG + chr3_1:74399345-74399364 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG + chr3_1:74399421-74399440 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG - chr3_1:74399418-74399437 Msa0374870:intron 40.0%
ACTGGTCCATCAGAAATTGA+TGG + chr3_1:74399427-74399446 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG + chr3_1:74399445-74399464 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG - chr3_1:74399444-74399463 Msa0374870:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG - chr3_1:74399455-74399474 Msa0374870:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG - chr3_1:74399458-74399477 Msa0374870:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG - chr3_1:74399608-74399627 Msa0374870:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG - chr3_1:74399673-74399692 Msa0374870:intron 40.0%
TCGTGATACATGTCCAATTG+AGG + chr3_1:74398993-74399012 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG - chr3_1:74399006-74399025 Msa0374870:three_prime_UTR 40.0%
GAGAGGACAAAAAGAACTCA+TGG - chr3_1:74399088-74399107 Msa0374870:three_prime_UTR 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG + chr3_1:74399178-74399197 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG - chr3_1:74399181-74399200 Msa0374870:exon 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG + chr3_1:74399186-74399205 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG - chr3_1:74399190-74399209 Msa0374870:CDS 40.0%
AAAAAGGTAACCCCACTTTC+AGG + chr3_1:74399345-74399364 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG + chr3_1:74399421-74399440 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG - chr3_1:74399418-74399437 Msa0374870:intron 40.0%
ACTGGTCCATCAGAAATTGA+TGG + chr3_1:74399427-74399446 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG + chr3_1:74399445-74399464 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG - chr3_1:74399444-74399463 Msa0374870:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG - chr3_1:74399455-74399474 Msa0374870:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG - chr3_1:74399458-74399477 Msa0374870:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG - chr3_1:74399608-74399627 Msa0374870:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG - chr3_1:74399673-74399692 Msa0374870:intron 40.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG + chr3_1:74398885-74398904 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG + chr3_1:74398981-74399000 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG + chr3_1:74399058-74399077 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG - chr3_1:74399058-74399077 Msa0374870:three_prime_UTR 45.0%
TGGCCTCTGTTCTTGAAACA+GGG + chr3_1:74399064-74399083 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG + chr3_1:74399065-74399084 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG - chr3_1:74399193-74399212 Msa0374870:CDS 45.0%
GGGAAAATTGATCGTGATCG+AGG - chr3_1:74399313-74399332 Msa0374870:intron 45.0%
TCGAGGTTTCTACCTGAAAG+TGG - chr3_1:74399330-74399349 Msa0374870:intron 45.0%
CGAGGTTTCTACCTGAAAGT+GGG - chr3_1:74399331-74399350 Msa0374870:intron 45.0%
GAGGTTTCTACCTGAAAGTG+GGG - chr3_1:74399332-74399351 Msa0374870:intron 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG - chr3_1:74399686-74399705 Msa0374870:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG - chr3_1:74399694-74399713 Msa0374870:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG + chr3_1:74399914-74399933 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG - chr3_1:74399925-74399944 Msa0374870:intron 45.0%
ATGAAATGCAGGCGTGAGAA+GGG - chr3_1:74400049-74400068 Msa0374870:CDS 45.0%
TCATGTTTAATGGCAGGGCA+TGG - chr3_1:74400353-74400372 Msa0374870:CDS 45.0%
CATGTTTAATGGCAGGGCAT+GGG - chr3_1:74400354-74400373 Msa0374870:CDS 45.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG + chr3_1:74398885-74398904 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG + chr3_1:74398981-74399000 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG + chr3_1:74399058-74399077 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG - chr3_1:74399058-74399077 Msa0374870:three_prime_UTR 45.0%
TGGCCTCTGTTCTTGAAACA+GGG + chr3_1:74399064-74399083 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG + chr3_1:74399065-74399084 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG - chr3_1:74399193-74399212 Msa0374870:CDS 45.0%
GGGAAAATTGATCGTGATCG+AGG - chr3_1:74399313-74399332 Msa0374870:intron 45.0%
TCGAGGTTTCTACCTGAAAG+TGG - chr3_1:74399330-74399349 Msa0374870:intron 45.0%
CGAGGTTTCTACCTGAAAGT+GGG - chr3_1:74399331-74399350 Msa0374870:intron 45.0%
GAGGTTTCTACCTGAAAGTG+GGG - chr3_1:74399332-74399351 Msa0374870:intron 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG - chr3_1:74399686-74399705 Msa0374870:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG - chr3_1:74399694-74399713 Msa0374870:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG + chr3_1:74399914-74399933 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG - chr3_1:74399925-74399944 Msa0374870:intron 45.0%
ATGAAATGCAGGCGTGAGAA+GGG - chr3_1:74400049-74400068 Msa0374870:CDS 45.0%
TCATGTTTAATGGCAGGGCA+TGG - chr3_1:74400353-74400372 Msa0374870:CDS 45.0%
CATGTTTAATGGCAGGGCAT+GGG - chr3_1:74400354-74400373 Msa0374870:CDS 45.0%
GGCTTCCCATGTTTCATGTC+TGG + chr3_1:74398937-74398956 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG - chr3_1:74398977-74398996 Msa0374870:three_prime_UTR 50.0%
GGTGAGTTCACACTCCATCT+AGG + chr3_1:74399023-74399042 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG + chr3_1:74399044-74399063 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG + chr3_1:74399063-74399082 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG - chr3_1:74399071-74399090 Msa0374870:three_prime_UTR 50.0%
ATCTTGTGGAACACCACCAC+AGG - chr3_1:74399159-74399178 Msa0374870:three_prime_UTR 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG + chr3_1:74399175-74399194 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG - chr3_1:74399526-74399545 Msa0374870:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG + chr3_1:74399915-74399934 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG - chr3_1:74399919-74399938 Msa0374870:intron 50.0%
GATGAAATGCAGGCGTGAGA+AGG - chr3_1:74400048-74400067 Msa0374870:CDS 50.0%
! AGAAGGGTGGAGGTATATGC+TGG - chr3_1:74400065-74400084 Msa0374870:CDS 50.0%
GGCTTCCCATGTTTCATGTC+TGG + chr3_1:74398937-74398956 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG - chr3_1:74398977-74398996 Msa0374870:three_prime_UTR 50.0%
GGTGAGTTCACACTCCATCT+AGG + chr3_1:74399023-74399042 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG + chr3_1:74399044-74399063 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG + chr3_1:74399063-74399082 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG - chr3_1:74399071-74399090 Msa0374870:three_prime_UTR 50.0%
ATCTTGTGGAACACCACCAC+AGG - chr3_1:74399159-74399178 Msa0374870:three_prime_UTR 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG + chr3_1:74399175-74399194 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG - chr3_1:74399526-74399545 Msa0374870:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG + chr3_1:74399915-74399934 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG - chr3_1:74399919-74399938 Msa0374870:intron 50.0%
GATGAAATGCAGGCGTGAGA+AGG - chr3_1:74400048-74400067 Msa0374870:CDS 50.0%
! AGAAGGGTGGAGGTATATGC+TGG - chr3_1:74400065-74400084 Msa0374870:CDS 50.0%
TACAGAGGAGCAGAAGGAGG+AGG - chr3_1:74399196-74399215 Msa0374870:CDS 55.0%
AGGAGGAGAGGAGAAGGAGA+AGG - chr3_1:74399216-74399235 Msa0374870:CDS 55.0%
AAATGCAGGCGTGAGAAGGG+TGG - chr3_1:74400052-74400071 Msa0374870:CDS 55.0%
GTTTAATGGCAGGGCATGGG+AGG - chr3_1:74400357-74400376 Msa0374870:CDS 55.0%
TACAGAGGAGCAGAAGGAGG+AGG - chr3_1:74399196-74399215 Msa0374870:CDS 55.0%
AGGAGGAGAGGAGAAGGAGA+AGG - chr3_1:74399216-74399235 Msa0374870:CDS 55.0%
AAATGCAGGCGTGAGAAGGG+TGG - chr3_1:74400052-74400071 Msa0374870:CDS 55.0%
GTTTAATGGCAGGGCATGGG+AGG - chr3_1:74400357-74400376 Msa0374870:CDS 55.0%
AGAGGAGCAGAAGGAGGAGG+AGG - chr3_1:74399199-74399218 Msa0374870:CDS 60.0%
AGCAGAAGGAGGAGGAGGAG+AGG - chr3_1:74399204-74399223 Msa0374870:CDS 60.0%
AGGAGGAGGAGGAGAGGAGA+AGG - chr3_1:74399210-74399229 Msa0374870:CDS 60.0%
AGAGGAGCAGAAGGAGGAGG+AGG - chr3_1:74399199-74399218 Msa0374870:CDS 60.0%
AGCAGAAGGAGGAGGAGGAG+AGG - chr3_1:74399204-74399223 Msa0374870:CDS 60.0%
AGGAGGAGGAGGAGAGGAGA+AGG - chr3_1:74399210-74399229 Msa0374870:CDS 60.0%
TGCAGGCGTGAGAAGGGTGG+AGG - chr3_1:74400055-74400074 Msa0374870:CDS 65.0%
TGCAGGCGTGAGAAGGGTGG+AGG - chr3_1:74400055-74400074 Msa0374870:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 74398873 74400439 74398873 ID=Msa0374870;Name=Msa0374870
chr3_1 mRNA 74398873 74400439 74398873 ID=Msa0374870-mRNA-1;Parent=Msa0374870;Name=Msa0374870-mRNA-1;_AED=0.19;_eAED=0.22;_QI=0|0|0.5|1|0|0|2|313|161
chr3_1 exon 74398873 74399252 74398873 ID=Msa0374870-mRNA-1:exon:17261;Parent=Msa0374870-mRNA-1
chr3_1 exon 74400021 74400439 74400021 ID=Msa0374870-mRNA-1:exon:17260;Parent=Msa0374870-mRNA-1
chr3_1 CDS 74400021 74400439 74400021 ID=Msa0374870-mRNA-1:cds;Parent=Msa0374870-mRNA-1
chr3_1 CDS 74399186 74399252 74399186 ID=Msa0374870-mRNA-1:cds;Parent=Msa0374870-mRNA-1
chr3_1 three_prime_UTR 74398873 74399185 74398873 ID=Msa0374870-mRNA-1:three_prime_utr;Parent=Msa0374870-mRNA-1
Gene Sequence

>Msa0374870

ATGCTTGATCCAACAAGAAAACACCACCAACCTAAGAATTCTTTTGATTTGTACGATGAAACCAGACATGAAACATGGGAAGCCACAAATTCTAATAATCTCTGGCCTTCAACACAGCCTCAATTGGACATGTATCACGATGAACAACCTAGATGGAGTGTGAACTCACCACATTACAGAAGCCTTTCCCCTGTTTCAAGAACAGAGGCCATAGTGAGAGGACAAAAAGAACTCATGGAAATGGTTCGGAACATGCCCGAATCAAACTATGAACTTTCATTGAAGGATCTTGTGGAACACCACCACAGGACCAGAGAAGAAAATACAGAGGAGCAGAAGGAGGAGGAGGAGAGGAGAAGGAGAAGGAGAAGAAGAATTCTAGTGTCTATAATGGTGGCAAGAAGATTGATAATAAGAAGGCGTGAGAAGGGTGGAGGTATATGCTGGCCTTTGTTTCGCAAACCCAAAATCCAAATAAAAAAGTAA
Protein sequence

>Msa0374870

MLDPTRKHHQPKNSFDLYDETRHETWEATNSNNLWPSTQPQLDMYHDEQPRWSVNSPHYRSLSPVSRTEAIVRGQKELMEMVRNMPESNYELSLKDLVEHHHRTREENTEEQKEEEERRRRRRRRILVSIMVARRLIIRRREKGGGICWPLFRKPKIQIKK*