AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0470360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0470360 MtrunA17_Chr3g0125561 84.021 194 19 3 1 193 31 213 1.47e-91 270
Msa0470360 MtrunA17_Chr1g0159571 42.308 208 85 8 1 190 1 191 5.56e-29 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0470360 AT1G21390.1 43.537 147 54 5 59 191 52 183 1.74e-17 78.6
Msa0470360 AT1G76980.1 41.463 164 62 7 50 191 33 184 3.60e-17 77.8
Msa0470360 AT1G76980.2 41.463 164 62 7 50 191 33 184 5.87e-17 77.0

Find 61 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATCATCATGTTTAA+TGG 0.197990 3_3:-72435707 Msa0470360:three_prime_UTR
GAATTAGACAACTTTGCTTC+TGG 0.302671 3_3:-72435763 Msa0470360:three_prime_UTR
ATTTCTGATGGACCAGTAAA+AGG 0.323657 3_3:-72436621 Msa0470360:intron
ATGGCCTCTGTTCTTGAAAC+AGG 0.336109 3_3:+72436984 None:intergenic
TACAAATCAAAAGAATTCTT+AGG 0.338959 3_3:+72437143 None:intergenic
GAAAGTTCATAGTTTGATTC+GGG 0.347032 3_3:+72436918 None:intergenic
CCAGAGAATATTAGAATTTG+TGG 0.349920 3_3:+72437091 None:intergenic
TGAAAGTTCATAGTTTGATT+CGG 0.368860 3_3:+72436917 None:intergenic
TTCTTTATATGTTTATTTGA+AGG 0.375267 3_3:-72435631 None:intergenic
GCAGAAGGAGGAGGAGGAGA+AGG 0.389816 3_3:-72436841 Msa0470360:CDS
GCCTTCAACACAGCCTCAAT+TGG 0.391567 3_3:-72437069 Msa0470360:CDS
GGCTTCCCATGTTTCATGTC+TGG 0.393616 3_3:+72437112 None:intergenic
AAACTATGAACTTTCATTGA+AGG 0.394782 3_3:-72436910 Msa0470360:CDS
ACTGGTCCATCAGAAATTGA+TGG 0.402064 3_3:+72436627 None:intergenic
AAGAATTCTAGTGTCTATAA+TGG 0.423589 3_3:-72436807 Msa0470360:CDS
AGCAAAGTTGTCTAATTCAA+TGG 0.432684 3_3:+72435768 None:intergenic
ATCACGATGAACAACCTAGA+TGG 0.455248 3_3:-72437040 Msa0470360:CDS
ATATCATCATGTTTAATGGC+AGG 0.462367 3_3:-72435703 Msa0470360:three_prime_UTR
CCACAAATTCTAATATTCTC+TGG 0.470611 3_3:-72437091 Msa0470360:CDS
GGAGGAGGAGGAGAAGGAGA+AGG 0.482742 3_3:-72436835 Msa0470360:CDS
AAGAACTCATGGAAATGGTT+CGG 0.483414 3_3:-72436947 Msa0470360:CDS
AGAAAATACAGAGGAGCAGA+AGG 0.490882 3_3:-72436856 Msa0470360:CDS
TCATGTTTAATGGCAGGGCA+TGG 0.506028 3_3:-72435697 Msa0470360:three_prime_UTR
AGAGGAGCAGAAGGAGGAGG+AGG 0.512087 3_3:-72436847 Msa0470360:CDS
CCATCAGAAATTGATGGTCT+TGG 0.516641 3_3:+72436633 None:intergenic
TCCAATTGAGGCTGTGTTGA+AGG 0.516902 3_3:+72437068 None:intergenic
GGTGAGTTCACACTCCATCT+AGG 0.517049 3_3:+72437026 None:intergenic
ATCTTGTGGAACACCACCAC+AGG 0.517846 3_3:-72436887 Msa0470360:CDS
ATAGTACTTTGTTTCATCAA+AGG 0.520728 3_3:+72435910 None:intergenic
ACTTTCATTGAAGGATCTTG+TGG 0.526030 3_3:-72436901 Msa0470360:CDS
TTACAAAGTTTGGATTGGCA+TGG 0.529260 3_3:+72435668 None:intergenic
TACAGAGGAGCAGAAGGAGG+AGG 0.532433 3_3:-72436850 Msa0470360:CDS
AATCAAAAGAATTCTTAGGT+TGG 0.534469 3_3:+72437147 None:intergenic
CCAAGACCATCAATTTCTGA+TGG 0.535937 3_3:-72436633 Msa0470360:CDS
CAAAAGAATTCTTAGGTTGG+TGG 0.537203 3_3:+72437150 None:intergenic
CGAGGTTTCTACCTGAAAGT+GGG 0.539143 3_3:-72436720 Msa0470360:CDS
TTTCTTCTCTGGTCCTGTGG+TGG 0.548885 3_3:+72436874 None:intergenic
CATGTTTAATGGCAGGGCAT+GGG 0.551016 3_3:-72435696 Msa0470360:three_prime_UTR
CAAGAAGATTGATAATAAGA+AGG 0.553116 3_3:-72436781 Msa0470360:CDS
GGGAAAATTGATCGTGATCG+AGG 0.558249 3_3:-72436738 Msa0470360:CDS
CTGTTCTTGAAACAGGGGAA+AGG 0.560820 3_3:+72436991 None:intergenic
TCGAGGTTTCTACCTGAAAG+TGG 0.562734 3_3:-72436721 Msa0470360:CDS
AGAAAGGTGGAGGTATATGC+TGG 0.563507 3_3:-72435985 Msa0470360:CDS
TCGTGATACATGTCCAATTG+AGG 0.569340 3_3:+72437056 None:intergenic
GTTTAATGGCAGGGCATGGG+AGG 0.571187 3_3:-72435693 Msa0470360:three_prime_UTR
TATCATCATGTTTAATGGCA+GGG 0.575049 3_3:-72435702 Msa0470360:three_prime_UTR
TGCAGGCGTGAGAAAGGTGG+AGG 0.582682 3_3:-72435995 Msa0470360:intron
ATGAAATGCAGGCGTGAGAA+AGG 0.589952 3_3:-72436001 Msa0470360:intron
AAATGCAGGCGTGAGAAAGG+TGG 0.592081 3_3:-72435998 Msa0470360:intron
TGGCCTCTGTTCTTGAAACA+GGG 0.597218 3_3:+72436985 None:intergenic
ATGTGATTTCATCATAGTCA+TGG 0.597220 3_3:-72435889 Msa0470360:three_prime_UTR
ATGAAACCAGACATGAAACA+TGG 0.602607 3_3:-72437118 Msa0470360:CDS
AGAACAGAGGCCATAGTGAG+AGG 0.606015 3_3:-72436975 Msa0470360:CDS
AATTCTAGTGTCTATAATGG+TGG 0.616757 3_3:-72436804 Msa0470360:CDS
TTCCCCTGTTTCAAGAACAG+AGG 0.619257 3_3:-72436988 Msa0470360:CDS
GGGGAAAGGCTTCTGTAATG+TGG 0.623224 3_3:+72437005 None:intergenic
AAATACAGAGGAGCAGAAGG+AGG 0.627616 3_3:-72436853 Msa0470360:CDS
GGCCTCTGTTCTTGAAACAG+GGG 0.643547 3_3:+72436986 None:intergenic
GAGGTTTCTACCTGAAAGTG+GGG 0.662564 3_3:-72436719 Msa0470360:CDS
TGAAACCAGACATGAAACAT+GGG 0.668487 3_3:-72437117 Msa0470360:CDS
GACCAGAGAAGAAAATACAG+AGG 0.687498 3_3:-72436865 Msa0470360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTAATTAATTTGAAGTT+GGG + chr3_3:72436398-72436417 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG + chr3_3:72436399-72436418 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG - chr3_3:72436528-72436547 Msa0470360:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG + chr3_3:72436862-72436881 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG + chr3_3:72436863-72436882 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG + chr3_3:72436870-72436889 None:intergenic 15.0%
!!! ATGTTAATTAATTTGAAGTT+GGG + chr3_3:72436398-72436417 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG + chr3_3:72436399-72436418 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG - chr3_3:72436528-72436547 Msa0470360:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG + chr3_3:72436862-72436881 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG + chr3_3:72436863-72436882 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG + chr3_3:72436870-72436889 None:intergenic 15.0%
!! TACAAATCAAAAGAATTCTT+AGG + chr3_3:72435666-72435685 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG + chr3_3:72436116-72436135 None:intergenic 20.0%
!! TAACAAAAATTTCATGTTCA+TGG - chr3_3:72436596-72436615 Msa0470360:intron 20.0%
!!! AAGAAAATAACTCTTGTTAT+TGG - chr3_3:72436712-72436731 Msa0470360:CDS 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG - chr3_3:72436755-72436774 Msa0470360:CDS 20.0%
!!! ATAGAGTACTAATTAACTTT+TGG - chr3_3:72436782-72436801 Msa0470360:CDS 20.0%
!! ACAAATATCATCATGTTTAA+TGG - chr3_3:72437099-72437118 Msa0470360:CDS 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG + chr3_3:72437146-72437165 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG + chr3_3:72437151-72437170 None:intergenic 20.0%
!! TACAAATCAAAAGAATTCTT+AGG + chr3_3:72435666-72435685 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG + chr3_3:72436116-72436135 None:intergenic 20.0%
!! TAACAAAAATTTCATGTTCA+TGG - chr3_3:72436596-72436615 Msa0470360:intron 20.0%
!!! AAGAAAATAACTCTTGTTAT+TGG - chr3_3:72436712-72436731 Msa0470360:CDS 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG - chr3_3:72436755-72436774 Msa0470360:CDS 20.0%
!!! ATAGAGTACTAATTAACTTT+TGG - chr3_3:72436782-72436801 Msa0470360:CDS 20.0%
!! ACAAATATCATCATGTTTAA+TGG - chr3_3:72437099-72437118 Msa0470360:CDS 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG + chr3_3:72437146-72437165 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG + chr3_3:72437151-72437170 None:intergenic 20.0%
! AATCAAAAGAATTCTTAGGT+TGG + chr3_3:72435662-72435681 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG + chr3_3:72435892-72435911 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG - chr3_3:72435896-72435915 Msa0470360:three_prime_UTR 25.0%
! AAGAATTCTAGTGTCTATAA+TGG - chr3_3:72435999-72436018 Msa0470360:intron 25.0%
!! CAAGAAGATTGATAATAAGA+AGG - chr3_3:72436025-72436044 Msa0470360:intron 25.0%
! TACATGTGAAGAAAAATGAT+GGG - chr3_3:72436048-72436067 Msa0470360:intron 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG - chr3_3:72436136-72436155 Msa0470360:intron 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG - chr3_3:72436421-72436440 Msa0470360:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG - chr3_3:72436630-72436649 Msa0470360:CDS 25.0%
! TAAGCAAAAAGACATGTATT+TGG + chr3_3:72436749-72436768 None:intergenic 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG + chr3_3:72436899-72436918 None:intergenic 25.0%
! AAAAAAAAGTTGTTCTCAGA+TGG + chr3_3:72436982-72437001 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG - chr3_3:72437065-72437084 Msa0470360:CDS 25.0%
! AATCAAAAGAATTCTTAGGT+TGG + chr3_3:72435662-72435681 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG + chr3_3:72435892-72435911 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG - chr3_3:72435896-72435915 Msa0470360:three_prime_UTR 25.0%
! AAGAATTCTAGTGTCTATAA+TGG - chr3_3:72435999-72436018 Msa0470360:intron 25.0%
!! CAAGAAGATTGATAATAAGA+AGG - chr3_3:72436025-72436044 Msa0470360:intron 25.0%
! TACATGTGAAGAAAAATGAT+GGG - chr3_3:72436048-72436067 Msa0470360:intron 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG - chr3_3:72436136-72436155 Msa0470360:intron 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG - chr3_3:72436421-72436440 Msa0470360:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG - chr3_3:72436630-72436649 Msa0470360:CDS 25.0%
! TAAGCAAAAAGACATGTATT+TGG + chr3_3:72436749-72436768 None:intergenic 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG + chr3_3:72436899-72436918 None:intergenic 25.0%
! AAAAAAAAGTTGTTCTCAGA+TGG + chr3_3:72436982-72437001 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG - chr3_3:72437065-72437084 Msa0470360:CDS 25.0%
CCAGAGAATATTAGAATTTG+TGG + chr3_3:72435718-72435737 None:intergenic 30.0%
CCACAAATTCTAATATTCTC+TGG - chr3_3:72435715-72435734 Msa0470360:three_prime_UTR 30.0%
ACAAAAAGAACTCATGGAAA+TGG - chr3_3:72435854-72435873 Msa0470360:three_prime_UTR 30.0%
GAAAGTTCATAGTTTGATTC+GGG + chr3_3:72435891-72435910 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG - chr3_3:72436002-72436021 Msa0470360:intron 30.0%
GTACATGTGAAGAAAAATGA+TGG - chr3_3:72436047-72436066 Msa0470360:intron 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG - chr3_3:72436107-72436126 Msa0470360:intron 30.0%
TCACTCTCTTTATAAACAGA+AGG + chr3_3:72436248-72436267 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG + chr3_3:72436460-72436479 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG - chr3_3:72436603-72436622 Msa0470360:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG + chr3_3:72436691-72436710 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG + chr3_3:72436692-72436711 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG - chr3_3:72436794-72436813 Msa0470360:CDS 30.0%
ATGTGATTTCATCATAGTCA+TGG - chr3_3:72436917-72436936 Msa0470360:CDS 30.0%
AGCAAAGTTGTCTAATTCAA+TGG + chr3_3:72437041-72437060 None:intergenic 30.0%
ATATCATCATGTTTAATGGC+AGG - chr3_3:72437103-72437122 Msa0470360:CDS 30.0%
TATCATCATGTTTAATGGCA+GGG - chr3_3:72437104-72437123 Msa0470360:CDS 30.0%
CCAGAGAATATTAGAATTTG+TGG + chr3_3:72435718-72435737 None:intergenic 30.0%
CCACAAATTCTAATATTCTC+TGG - chr3_3:72435715-72435734 Msa0470360:three_prime_UTR 30.0%
ACAAAAAGAACTCATGGAAA+TGG - chr3_3:72435854-72435873 Msa0470360:three_prime_UTR 30.0%
GAAAGTTCATAGTTTGATTC+GGG + chr3_3:72435891-72435910 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG - chr3_3:72436002-72436021 Msa0470360:intron 30.0%
GTACATGTGAAGAAAAATGA+TGG - chr3_3:72436047-72436066 Msa0470360:intron 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG - chr3_3:72436107-72436126 Msa0470360:intron 30.0%
TCACTCTCTTTATAAACAGA+AGG + chr3_3:72436248-72436267 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG + chr3_3:72436460-72436479 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG - chr3_3:72436603-72436622 Msa0470360:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG + chr3_3:72436691-72436710 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG + chr3_3:72436692-72436711 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG - chr3_3:72436794-72436813 Msa0470360:CDS 30.0%
ATGTGATTTCATCATAGTCA+TGG - chr3_3:72436917-72436936 Msa0470360:CDS 30.0%
AGCAAAGTTGTCTAATTCAA+TGG + chr3_3:72437041-72437060 None:intergenic 30.0%
ATATCATCATGTTTAATGGC+AGG - chr3_3:72437103-72437122 Msa0470360:CDS 30.0%
TATCATCATGTTTAATGGCA+GGG - chr3_3:72437104-72437123 Msa0470360:CDS 30.0%
!! CAAAAGAATTCTTAGGTTGG+TGG + chr3_3:72435659-72435678 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG - chr3_3:72435688-72435707 Msa0470360:three_prime_UTR 35.0%
TGAAACCAGACATGAAACAT+GGG - chr3_3:72435689-72435708 Msa0470360:three_prime_UTR 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG + chr3_3:72435844-72435863 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG - chr3_3:72435859-72435878 Msa0470360:three_prime_UTR 35.0%
ACTTTCATTGAAGGATCTTG+TGG - chr3_3:72435905-72435924 Msa0470360:three_prime_UTR 35.0%
! ATTTCTGATGGACCAGTAAA+AGG - chr3_3:72436185-72436204 Msa0470360:intron 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG - chr3_3:72436429-72436448 Msa0470360:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG - chr3_3:72436654-72436673 Msa0470360:CDS 35.0%
AATCAAATCCCATGTTGGAT+TGG - chr3_3:72436659-72436678 Msa0470360:CDS 35.0%
ATCAAATCCCATGTTGGATT+GGG - chr3_3:72436660-72436679 Msa0470360:CDS 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG + chr3_3:72436847-72436866 None:intergenic 35.0%
GAATTAGACAACTTTGCTTC+TGG - chr3_3:72437043-72437062 Msa0470360:CDS 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG + chr3_3:72437141-72437160 None:intergenic 35.0%
!! CAAAAGAATTCTTAGGTTGG+TGG + chr3_3:72435659-72435678 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG - chr3_3:72435688-72435707 Msa0470360:three_prime_UTR 35.0%
TGAAACCAGACATGAAACAT+GGG - chr3_3:72435689-72435708 Msa0470360:three_prime_UTR 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG + chr3_3:72435844-72435863 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG - chr3_3:72435859-72435878 Msa0470360:three_prime_UTR 35.0%
ACTTTCATTGAAGGATCTTG+TGG - chr3_3:72435905-72435924 Msa0470360:three_prime_UTR 35.0%
! ATTTCTGATGGACCAGTAAA+AGG - chr3_3:72436185-72436204 Msa0470360:intron 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG - chr3_3:72436429-72436448 Msa0470360:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG - chr3_3:72436654-72436673 Msa0470360:CDS 35.0%
AATCAAATCCCATGTTGGAT+TGG - chr3_3:72436659-72436678 Msa0470360:CDS 35.0%
ATCAAATCCCATGTTGGATT+GGG - chr3_3:72436660-72436679 Msa0470360:CDS 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG + chr3_3:72436847-72436866 None:intergenic 35.0%
GAATTAGACAACTTTGCTTC+TGG - chr3_3:72437043-72437062 Msa0470360:CDS 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG + chr3_3:72437141-72437160 None:intergenic 35.0%
TCGTGATACATGTCCAATTG+AGG + chr3_3:72435753-72435772 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG - chr3_3:72435766-72435785 Msa0470360:three_prime_UTR 40.0%
GAGAGGACAAAAAGAACTCA+TGG - chr3_3:72435848-72435867 Msa0470360:three_prime_UTR 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG + chr3_3:72435938-72435957 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG - chr3_3:72435941-72435960 Msa0470360:exon 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG + chr3_3:72435946-72435965 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG - chr3_3:72435950-72435969 Msa0470360:exon 40.0%
AAAAAGGTAACCCCACTTTC+AGG + chr3_3:72436100-72436119 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG + chr3_3:72436176-72436195 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG - chr3_3:72436173-72436192 Msa0470360:intron 40.0%
ACTGGTCCATCAGAAATTGA+TGG + chr3_3:72436182-72436201 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG + chr3_3:72436200-72436219 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG - chr3_3:72436199-72436218 Msa0470360:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG - chr3_3:72436210-72436229 Msa0470360:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG - chr3_3:72436213-72436232 Msa0470360:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG - chr3_3:72436363-72436382 Msa0470360:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG - chr3_3:72436428-72436447 Msa0470360:intron 40.0%
TCGTGATACATGTCCAATTG+AGG + chr3_3:72435753-72435772 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG - chr3_3:72435766-72435785 Msa0470360:three_prime_UTR 40.0%
GAGAGGACAAAAAGAACTCA+TGG - chr3_3:72435848-72435867 Msa0470360:three_prime_UTR 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG + chr3_3:72435938-72435957 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG - chr3_3:72435941-72435960 Msa0470360:exon 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG + chr3_3:72435946-72435965 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG - chr3_3:72435950-72435969 Msa0470360:exon 40.0%
AAAAAGGTAACCCCACTTTC+AGG + chr3_3:72436100-72436119 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG + chr3_3:72436176-72436195 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG - chr3_3:72436173-72436192 Msa0470360:intron 40.0%
ACTGGTCCATCAGAAATTGA+TGG + chr3_3:72436182-72436201 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG + chr3_3:72436200-72436219 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG - chr3_3:72436199-72436218 Msa0470360:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG - chr3_3:72436210-72436229 Msa0470360:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG - chr3_3:72436213-72436232 Msa0470360:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG - chr3_3:72436363-72436382 Msa0470360:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG - chr3_3:72436428-72436447 Msa0470360:intron 40.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG + chr3_3:72435645-72435664 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG + chr3_3:72435741-72435760 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG + chr3_3:72435818-72435837 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG - chr3_3:72435818-72435837 Msa0470360:three_prime_UTR 45.0%
TGGCCTCTGTTCTTGAAACA+GGG + chr3_3:72435824-72435843 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG + chr3_3:72435825-72435844 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG - chr3_3:72435953-72435972 Msa0470360:exon 45.0%
GGGAAAATTGATCGTGATCG+AGG - chr3_3:72436068-72436087 Msa0470360:intron 45.0%
TCGAGGTTTCTACCTGAAAG+TGG - chr3_3:72436085-72436104 Msa0470360:intron 45.0%
CGAGGTTTCTACCTGAAAGT+GGG - chr3_3:72436086-72436105 Msa0470360:intron 45.0%
GAGGTTTCTACCTGAAAGTG+GGG - chr3_3:72436087-72436106 Msa0470360:intron 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG - chr3_3:72436441-72436460 Msa0470360:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG - chr3_3:72436449-72436468 Msa0470360:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG + chr3_3:72436670-72436689 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG - chr3_3:72436681-72436700 Msa0470360:CDS 45.0%
ATGAAATGCAGGCGTGAGAA+AGG - chr3_3:72436805-72436824 Msa0470360:CDS 45.0%
! AGAAAGGTGGAGGTATATGC+TGG - chr3_3:72436821-72436840 Msa0470360:CDS 45.0%
TCATGTTTAATGGCAGGGCA+TGG - chr3_3:72437109-72437128 Msa0470360:CDS 45.0%
CATGTTTAATGGCAGGGCAT+GGG - chr3_3:72437110-72437129 Msa0470360:CDS 45.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG + chr3_3:72435645-72435664 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG + chr3_3:72435741-72435760 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG + chr3_3:72435818-72435837 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG - chr3_3:72435818-72435837 Msa0470360:three_prime_UTR 45.0%
TGGCCTCTGTTCTTGAAACA+GGG + chr3_3:72435824-72435843 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG + chr3_3:72435825-72435844 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG - chr3_3:72435953-72435972 Msa0470360:exon 45.0%
GGGAAAATTGATCGTGATCG+AGG - chr3_3:72436068-72436087 Msa0470360:intron 45.0%
TCGAGGTTTCTACCTGAAAG+TGG - chr3_3:72436085-72436104 Msa0470360:intron 45.0%
CGAGGTTTCTACCTGAAAGT+GGG - chr3_3:72436086-72436105 Msa0470360:intron 45.0%
GAGGTTTCTACCTGAAAGTG+GGG - chr3_3:72436087-72436106 Msa0470360:intron 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG - chr3_3:72436441-72436460 Msa0470360:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG - chr3_3:72436449-72436468 Msa0470360:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG + chr3_3:72436670-72436689 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG - chr3_3:72436681-72436700 Msa0470360:CDS 45.0%
ATGAAATGCAGGCGTGAGAA+AGG - chr3_3:72436805-72436824 Msa0470360:CDS 45.0%
! AGAAAGGTGGAGGTATATGC+TGG - chr3_3:72436821-72436840 Msa0470360:CDS 45.0%
TCATGTTTAATGGCAGGGCA+TGG - chr3_3:72437109-72437128 Msa0470360:CDS 45.0%
CATGTTTAATGGCAGGGCAT+GGG - chr3_3:72437110-72437129 Msa0470360:CDS 45.0%
GGCTTCCCATGTTTCATGTC+TGG + chr3_3:72435697-72435716 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG - chr3_3:72435737-72435756 Msa0470360:three_prime_UTR 50.0%
GGTGAGTTCACACTCCATCT+AGG + chr3_3:72435783-72435802 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG + chr3_3:72435804-72435823 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG + chr3_3:72435823-72435842 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG - chr3_3:72435831-72435850 Msa0470360:three_prime_UTR 50.0%
ATCTTGTGGAACACCACCAC+AGG - chr3_3:72435919-72435938 Msa0470360:three_prime_UTR 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG + chr3_3:72435935-72435954 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG - chr3_3:72436281-72436300 Msa0470360:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG + chr3_3:72436671-72436690 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG - chr3_3:72436675-72436694 Msa0470360:CDS 50.0%
AAATGCAGGCGTGAGAAAGG+TGG - chr3_3:72436808-72436827 Msa0470360:CDS 50.0%
GGCTTCCCATGTTTCATGTC+TGG + chr3_3:72435697-72435716 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG - chr3_3:72435737-72435756 Msa0470360:three_prime_UTR 50.0%
GGTGAGTTCACACTCCATCT+AGG + chr3_3:72435783-72435802 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG + chr3_3:72435804-72435823 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG + chr3_3:72435823-72435842 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG - chr3_3:72435831-72435850 Msa0470360:three_prime_UTR 50.0%
ATCTTGTGGAACACCACCAC+AGG - chr3_3:72435919-72435938 Msa0470360:three_prime_UTR 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG + chr3_3:72435935-72435954 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG - chr3_3:72436281-72436300 Msa0470360:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG + chr3_3:72436671-72436690 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG - chr3_3:72436675-72436694 Msa0470360:CDS 50.0%
AAATGCAGGCGTGAGAAAGG+TGG - chr3_3:72436808-72436827 Msa0470360:CDS 50.0%
TACAGAGGAGCAGAAGGAGG+AGG - chr3_3:72435956-72435975 Msa0470360:CDS 55.0%
GTTTAATGGCAGGGCATGGG+AGG - chr3_3:72437113-72437132 Msa0470360:CDS 55.0%
TACAGAGGAGCAGAAGGAGG+AGG - chr3_3:72435956-72435975 Msa0470360:CDS 55.0%
GTTTAATGGCAGGGCATGGG+AGG - chr3_3:72437113-72437132 Msa0470360:CDS 55.0%
AGAGGAGCAGAAGGAGGAGG+AGG - chr3_3:72435959-72435978 Msa0470360:CDS 60.0%
GCAGAAGGAGGAGGAGGAGA+AGG - chr3_3:72435965-72435984 Msa0470360:CDS 60.0%
GGAGGAGGAGGAGAAGGAGA+AGG - chr3_3:72435971-72435990 Msa0470360:CDS 60.0%
TGCAGGCGTGAGAAAGGTGG+AGG - chr3_3:72436811-72436830 Msa0470360:CDS 60.0%
AGAGGAGCAGAAGGAGGAGG+AGG - chr3_3:72435959-72435978 Msa0470360:CDS 60.0%
GCAGAAGGAGGAGGAGGAGA+AGG - chr3_3:72435965-72435984 Msa0470360:CDS 60.0%
GGAGGAGGAGGAGAAGGAGA+AGG - chr3_3:72435971-72435990 Msa0470360:CDS 60.0%
TGCAGGCGTGAGAAAGGTGG+AGG - chr3_3:72436811-72436830 Msa0470360:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 72435633 72437195 72435633 ID=Msa0470360;Name=Msa0470360
chr3_3 mRNA 72435633 72437195 72435633 ID=Msa0470360-mRNA-1;Parent=Msa0470360;Name=Msa0470360-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0.5|1|0|1|2|321|210
chr3_3 exon 72435633 72436012 72435633 ID=Msa0470360-mRNA-1:exon:18105;Parent=Msa0470360-mRNA-1
chr3_3 exon 72436622 72437195 72436622 ID=Msa0470360-mRNA-1:exon:18104;Parent=Msa0470360-mRNA-1
chr3_3 CDS 72436622 72437195 72436622 ID=Msa0470360-mRNA-1:cds;Parent=Msa0470360-mRNA-1
chr3_3 CDS 72435954 72436012 72435954 ID=Msa0470360-mRNA-1:cds;Parent=Msa0470360-mRNA-1
chr3_3 three_prime_UTR 72435633 72435953 72435633 ID=Msa0470360-mRNA-1:three_prime_utr;Parent=Msa0470360-mRNA-1
Gene Sequence

>Msa0470360

ATGCTTGATCCAACAAGAAAACACCACCAACCTAAGAATTCTTTTGATTTGTACGATGAAACCAGACATGAAACATGGGAAGCCACAAATTCTAATATTCTCTGGCCTTCAACACAGCCTCAATTGGACATGTATCACGATGAACAACCTAGATGGAGTGTGAACTCACCACATTACAGAAGCCTTTCCCCTGTTTCAAGAACAGAGGCCATAGTGAGAGGACAAAAAGAACTCATGGAAATGGTTCGGAACATGCCCGAATCAAACTATGAACTTTCATTGAAGGATCTTGTGGAACACCACCACAGGACCAGAGAAGAAAATACAGAGGAGCAGAAGGAGGAGGAGGAGAAGGAGAAGGAGAAGAAGAATTCTAGTGTCTATAATGGTGGCAAGAAGATTGATAATAAGAAGGTACATGTGAAGAAAAATGATGGGAAAATTGATCGTGATCGAGGTTTCTACCTGAAAGTGGGGTTACCTTTTTTTTCTTTAGGATCTAAAGAGAAAAAGAAGAAGAAAAAGGAGTCAAAAGTTTCACCAAGACCATCAATTTCTGATGGACCAGTAAAAGGCGTGAGAAAGGTGGAGGTATATGCTGGCCTTTGTTTCGCAAACCCAAAATCCAAATAA
Protein sequence

>Msa0470360

MLDPTRKHHQPKNSFDLYDETRHETWEATNSNILWPSTQPQLDMYHDEQPRWSVNSPHYRSLSPVSRTEAIVRGQKELMEMVRNMPESNYELSLKDLVEHHHRTREENTEEQKEEEEKEKEKKNSSVYNGGKKIDNKKVHVKKNDGKIDRDRGFYLKVGLPFFSLGSKEKKKKKKESKVSPRPSISDGPVKGVRKVEVYAGLCFANPKSK*