AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0375070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0375070 MtrunA17_Chr3g0125561 84.536 194 18 3 1 193 31 213 7.90e-93 273
Msa0375070 MtrunA17_Chr1g0159571 44.444 207 82 9 1 190 1 191 3.51e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0375070 AT1G21390.1 43.537 147 54 5 59 191 52 183 1.39e-17 79.0
Msa0375070 AT1G76980.1 41.463 164 62 7 50 191 33 184 3.35e-17 77.8
Msa0375070 AT1G76980.2 41.463 164 62 7 50 191 33 184 4.55e-17 77.4

Find 62 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATCATCATGTTTAA+TGG 0.197990 3_1:+74680346 Msa0375070:three_prime_UTR
GAATTAGATAACTTTGCTTC+TGG 0.283440 3_1:+74680290 Msa0375070:three_prime_UTR
ATTTCTGATGGACCAGTAAA+AGG 0.323657 3_1:+74679433 Msa0375070:CDS
ATGGCCTCTGTTCTTGAAAC+AGG 0.336109 3_1:-74679070 None:intergenic
TACAAATCAAAAGAATTCTT+AGG 0.338959 3_1:-74678911 None:intergenic
GAAAGTTCATAGTTTGATTC+GGG 0.347032 3_1:-74679136 None:intergenic
CCAGAGATTATTAGAATTTG+TGG 0.349920 3_1:-74678963 None:intergenic
TGAAAGTTCATAGTTTGATT+CGG 0.368860 3_1:-74679137 None:intergenic
TTCTTTATATGTTTATTTGA+AGG 0.375267 3_1:+74680422 Msa0375070:three_prime_UTR
GCAGAAGGAGGAGGAGGAGA+AGG 0.389816 3_1:+74679213 Msa0375070:CDS
GCCTTCAACACAGCCTCAAT+TGG 0.391567 3_1:+74678985 Msa0375070:CDS
GGCTTCCCATGTTTCATGTC+TGG 0.393616 3_1:-74678942 None:intergenic
AAACTATGAACTTTCATTGA+AGG 0.394782 3_1:+74679144 Msa0375070:CDS
ACTGGTCCATCAGAAATTGA+TGG 0.402064 3_1:-74679427 None:intergenic
AAGAATTCTAGTGTCTATAA+TGG 0.423589 3_1:+74679247 Msa0375070:CDS
ATCACGATGAACAACCTAGA+TGG 0.455248 3_1:+74679014 Msa0375070:CDS
AGCAAAGTTATCTAATTCAA+TGG 0.455720 3_1:-74680285 None:intergenic
ATATCATCATGTTTAATGGC+AGG 0.462367 3_1:+74680350 Msa0375070:three_prime_UTR
CCACAAATTCTAATAATCTC+TGG 0.468437 3_1:+74678963 Msa0375070:CDS
GGAGGAGGAGGAGAAGGAGA+AGG 0.482742 3_1:+74679219 Msa0375070:CDS
AAGAACTCATGGAAATGGTT+CGG 0.483414 3_1:+74679107 Msa0375070:CDS
AGAAAATACAGAGGAGCAGA+AGG 0.490882 3_1:+74679198 Msa0375070:CDS
TCATGTTTAATGGCAGGGCA+TGG 0.506028 3_1:+74680356 Msa0375070:three_prime_UTR
AGAGGAGCAGAAGGAGGAGG+AGG 0.512087 3_1:+74679207 Msa0375070:CDS
CCATCAGAAATTGATGGTCT+TGG 0.516641 3_1:-74679421 None:intergenic
TCCAATTGAGGCTGTGTTGA+AGG 0.516902 3_1:-74678986 None:intergenic
GGTGAGTTCACACTCCATCT+AGG 0.517049 3_1:-74679028 None:intergenic
ATCTTGTGGAACACCACCAC+AGG 0.517846 3_1:+74679167 Msa0375070:CDS
ATAGTACTTTGTTTCATCAA+AGG 0.520728 3_1:-74680143 None:intergenic
ACTTTCATTGAAGGATCTTG+TGG 0.526030 3_1:+74679153 Msa0375070:CDS
CTTTGTTTCATCAAAGGCAT+CGG 0.528423 3_1:-74680137 None:intergenic
TTACAAAGTTTGGATTGGCA+TGG 0.529260 3_1:-74680385 None:intergenic
TACAGAGGAGCAGAAGGAGG+AGG 0.532433 3_1:+74679204 Msa0375070:CDS
AATCAAAAGAATTCTTAGGT+TGG 0.534469 3_1:-74678907 None:intergenic
CCAAGACCATCAATTTCTGA+TGG 0.535937 3_1:+74679421 Msa0375070:CDS
CAAAAGAATTCTTAGGTTGG+TGG 0.537203 3_1:-74678904 None:intergenic
CGAGGTTTCTACCTGAAAGT+GGG 0.539143 3_1:+74679334 Msa0375070:CDS
TTTCTTCTCTGGTCCTGTGG+TGG 0.548885 3_1:-74679180 None:intergenic
CATGTTTAATGGCAGGGCAT+GGG 0.551016 3_1:+74680357 Msa0375070:three_prime_UTR
CAAGAAGATTGATAATAAGA+AGG 0.553116 3_1:+74679273 Msa0375070:CDS
GGGAAAATTGATCGTGATCG+AGG 0.558249 3_1:+74679316 Msa0375070:CDS
CTGTTCTTGAAACAGGGGAA+AGG 0.560820 3_1:-74679063 None:intergenic
AGAAGGGTGGAGGTATATGC+TGG 0.561488 3_1:+74680068 Msa0375070:CDS
TGCAGGCGTGAGAAGGGTGG+AGG 0.562031 3_1:+74680058 Msa0375070:intron
TCGAGGTTTCTACCTGAAAG+TGG 0.562734 3_1:+74679333 Msa0375070:CDS
TCGTGATACATGTCCAATTG+AGG 0.569340 3_1:-74678998 None:intergenic
GTTTAATGGCAGGGCATGGG+AGG 0.571187 3_1:+74680360 Msa0375070:three_prime_UTR
TATCATCATGTTTAATGGCA+GGG 0.575049 3_1:+74680351 Msa0375070:three_prime_UTR
TGGCCTCTGTTCTTGAAACA+GGG 0.597218 3_1:-74679069 None:intergenic
ATGAAACCAGACATGAAACA+TGG 0.602607 3_1:+74678936 Msa0375070:CDS
AGAACAGAGGCCATAGTGAG+AGG 0.606015 3_1:+74679079 Msa0375070:CDS
AAATGCAGGCGTGAGAAGGG+TGG 0.606501 3_1:+74680055 Msa0375070:intron
AATTCTAGTGTCTATAATGG+TGG 0.616757 3_1:+74679250 Msa0375070:CDS
TTCCCCTGTTTCAAGAACAG+AGG 0.619257 3_1:+74679066 Msa0375070:CDS
GGGGAAAGGCTTCTGTAATG+TGG 0.623224 3_1:-74679049 None:intergenic
AAATACAGAGGAGCAGAAGG+AGG 0.627616 3_1:+74679201 Msa0375070:CDS
GATGAAATGCAGGCGTGAGA+AGG 0.636676 3_1:+74680051 Msa0375070:intron
ATGAAATGCAGGCGTGAGAA+GGG 0.643514 3_1:+74680052 Msa0375070:intron
GGCCTCTGTTCTTGAAACAG+GGG 0.643547 3_1:-74679068 None:intergenic
GAGGTTTCTACCTGAAAGTG+GGG 0.662564 3_1:+74679335 Msa0375070:CDS
TGAAACCAGACATGAAACAT+GGG 0.668487 3_1:+74678937 Msa0375070:CDS
GACCAGAGAAGAAAATACAG+AGG 0.687498 3_1:+74679189 Msa0375070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTAATTAATTTGAAGTT+GGG - chr3_1:74679646-74679665 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG - chr3_1:74679647-74679666 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG + chr3_1:74679776-74679795 Msa0375070:intron 15.0%
!!! TAAGAAAATAACTCTTTTAT+TGG + chr3_1:74679959-74679978 Msa0375070:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG - chr3_1:74680109-74680128 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG - chr3_1:74680110-74680129 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG - chr3_1:74680117-74680136 None:intergenic 15.0%
!!! ATGTTAATTAATTTGAAGTT+GGG - chr3_1:74679646-74679665 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG - chr3_1:74679647-74679666 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG + chr3_1:74679776-74679795 Msa0375070:intron 15.0%
!!! TAAGAAAATAACTCTTTTAT+TGG + chr3_1:74679959-74679978 Msa0375070:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG - chr3_1:74680109-74680128 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG - chr3_1:74680110-74680129 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG - chr3_1:74680117-74680136 None:intergenic 15.0%
!! TACAAATCAAAAGAATTCTT+AGG - chr3_1:74678914-74678933 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG - chr3_1:74679364-74679383 None:intergenic 20.0%
!! TAACAAAAATTTCATGTTCA+TGG + chr3_1:74679844-74679863 Msa0375070:intron 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG + chr3_1:74680002-74680021 Msa0375070:intron 20.0%
!! AAAAAAAAATTGTTCTCAGA+TGG - chr3_1:74680229-74680248 None:intergenic 20.0%
!! ACAAATATCATCATGTTTAA+TGG + chr3_1:74680346-74680365 Msa0375070:three_prime_UTR 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG - chr3_1:74680393-74680412 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG - chr3_1:74680398-74680417 None:intergenic 20.0%
!! TACAAATCAAAAGAATTCTT+AGG - chr3_1:74678914-74678933 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG - chr3_1:74679364-74679383 None:intergenic 20.0%
!! TAACAAAAATTTCATGTTCA+TGG + chr3_1:74679844-74679863 Msa0375070:intron 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG + chr3_1:74680002-74680021 Msa0375070:intron 20.0%
!! AAAAAAAAATTGTTCTCAGA+TGG - chr3_1:74680229-74680248 None:intergenic 20.0%
!! ACAAATATCATCATGTTTAA+TGG + chr3_1:74680346-74680365 Msa0375070:three_prime_UTR 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG - chr3_1:74680393-74680412 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG - chr3_1:74680398-74680417 None:intergenic 20.0%
! AATCAAAAGAATTCTTAGGT+TGG - chr3_1:74678910-74678929 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG - chr3_1:74679140-74679159 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG + chr3_1:74679144-74679163 Msa0375070:CDS 25.0%
! AAGAATTCTAGTGTCTATAA+TGG + chr3_1:74679247-74679266 Msa0375070:CDS 25.0%
!! CAAGAAGATTGATAATAAGA+AGG + chr3_1:74679273-74679292 Msa0375070:CDS 25.0%
! TACATGTGAAGAAAAATGAT+GGG + chr3_1:74679296-74679315 Msa0375070:CDS 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG + chr3_1:74679384-74679403 Msa0375070:CDS 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG + chr3_1:74679669-74679688 Msa0375070:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG + chr3_1:74679878-74679897 Msa0375070:intron 25.0%
! TAAGCAAAAAGACATGTATT+TGG - chr3_1:74679996-74680015 None:intergenic 25.0%
!!! ATAGAGTACTGATTAACTTT+TGG + chr3_1:74680029-74680048 Msa0375070:intron 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG - chr3_1:74680146-74680165 None:intergenic 25.0%
! AGCAAAGTTATCTAATTCAA+TGG - chr3_1:74680288-74680307 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG + chr3_1:74680312-74680331 Msa0375070:three_prime_UTR 25.0%
! AATCAAAAGAATTCTTAGGT+TGG - chr3_1:74678910-74678929 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG - chr3_1:74679140-74679159 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG + chr3_1:74679144-74679163 Msa0375070:CDS 25.0%
! AAGAATTCTAGTGTCTATAA+TGG + chr3_1:74679247-74679266 Msa0375070:CDS 25.0%
!! CAAGAAGATTGATAATAAGA+AGG + chr3_1:74679273-74679292 Msa0375070:CDS 25.0%
! TACATGTGAAGAAAAATGAT+GGG + chr3_1:74679296-74679315 Msa0375070:CDS 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG + chr3_1:74679384-74679403 Msa0375070:CDS 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG + chr3_1:74679669-74679688 Msa0375070:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG + chr3_1:74679878-74679897 Msa0375070:intron 25.0%
! TAAGCAAAAAGACATGTATT+TGG - chr3_1:74679996-74680015 None:intergenic 25.0%
!!! ATAGAGTACTGATTAACTTT+TGG + chr3_1:74680029-74680048 Msa0375070:intron 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG - chr3_1:74680146-74680165 None:intergenic 25.0%
! AGCAAAGTTATCTAATTCAA+TGG - chr3_1:74680288-74680307 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG + chr3_1:74680312-74680331 Msa0375070:three_prime_UTR 25.0%
CCAGAGATTATTAGAATTTG+TGG - chr3_1:74678966-74678985 None:intergenic 30.0%
CCACAAATTCTAATAATCTC+TGG + chr3_1:74678963-74678982 Msa0375070:CDS 30.0%
ACAAAAAGAACTCATGGAAA+TGG + chr3_1:74679102-74679121 Msa0375070:CDS 30.0%
GAAAGTTCATAGTTTGATTC+GGG - chr3_1:74679139-74679158 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG + chr3_1:74679250-74679269 Msa0375070:CDS 30.0%
GTACATGTGAAGAAAAATGA+TGG + chr3_1:74679295-74679314 Msa0375070:CDS 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG + chr3_1:74679355-74679374 Msa0375070:CDS 30.0%
TCACTCTCTTTATAAACAGA+AGG - chr3_1:74679496-74679515 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG - chr3_1:74679708-74679727 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG + chr3_1:74679851-74679870 Msa0375070:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG - chr3_1:74679939-74679958 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG - chr3_1:74679940-74679959 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG + chr3_1:74680041-74680060 Msa0375070:intron 30.0%
GAATTAGATAACTTTGCTTC+TGG + chr3_1:74680290-74680309 Msa0375070:three_prime_UTR 30.0%
ATATCATCATGTTTAATGGC+AGG + chr3_1:74680350-74680369 Msa0375070:three_prime_UTR 30.0%
TATCATCATGTTTAATGGCA+GGG + chr3_1:74680351-74680370 Msa0375070:three_prime_UTR 30.0%
CCAGAGATTATTAGAATTTG+TGG - chr3_1:74678966-74678985 None:intergenic 30.0%
CCACAAATTCTAATAATCTC+TGG + chr3_1:74678963-74678982 Msa0375070:CDS 30.0%
ACAAAAAGAACTCATGGAAA+TGG + chr3_1:74679102-74679121 Msa0375070:CDS 30.0%
GAAAGTTCATAGTTTGATTC+GGG - chr3_1:74679139-74679158 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG + chr3_1:74679250-74679269 Msa0375070:CDS 30.0%
GTACATGTGAAGAAAAATGA+TGG + chr3_1:74679295-74679314 Msa0375070:CDS 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG + chr3_1:74679355-74679374 Msa0375070:CDS 30.0%
TCACTCTCTTTATAAACAGA+AGG - chr3_1:74679496-74679515 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG - chr3_1:74679708-74679727 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG + chr3_1:74679851-74679870 Msa0375070:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG - chr3_1:74679939-74679958 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG - chr3_1:74679940-74679959 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG + chr3_1:74680041-74680060 Msa0375070:intron 30.0%
GAATTAGATAACTTTGCTTC+TGG + chr3_1:74680290-74680309 Msa0375070:three_prime_UTR 30.0%
ATATCATCATGTTTAATGGC+AGG + chr3_1:74680350-74680369 Msa0375070:three_prime_UTR 30.0%
TATCATCATGTTTAATGGCA+GGG + chr3_1:74680351-74680370 Msa0375070:three_prime_UTR 30.0%
!! CAAAAGAATTCTTAGGTTGG+TGG - chr3_1:74678907-74678926 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG + chr3_1:74678936-74678955 Msa0375070:CDS 35.0%
TGAAACCAGACATGAAACAT+GGG + chr3_1:74678937-74678956 Msa0375070:CDS 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG - chr3_1:74679092-74679111 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG + chr3_1:74679107-74679126 Msa0375070:CDS 35.0%
ACTTTCATTGAAGGATCTTG+TGG + chr3_1:74679153-74679172 Msa0375070:CDS 35.0%
! ATTTCTGATGGACCAGTAAA+AGG + chr3_1:74679433-74679452 Msa0375070:CDS 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG + chr3_1:74679677-74679696 Msa0375070:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG + chr3_1:74679902-74679921 Msa0375070:intron 35.0%
AATCAAATCCCATGTTGGAT+TGG + chr3_1:74679907-74679926 Msa0375070:intron 35.0%
ATCAAATCCCATGTTGGATT+GGG + chr3_1:74679908-74679927 Msa0375070:intron 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG - chr3_1:74680094-74680113 None:intergenic 35.0%
! CTTTGTTTCATCAAAGGCAT+CGG - chr3_1:74680140-74680159 None:intergenic 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG - chr3_1:74680388-74680407 None:intergenic 35.0%
!! CAAAAGAATTCTTAGGTTGG+TGG - chr3_1:74678907-74678926 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG + chr3_1:74678936-74678955 Msa0375070:CDS 35.0%
TGAAACCAGACATGAAACAT+GGG + chr3_1:74678937-74678956 Msa0375070:CDS 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG - chr3_1:74679092-74679111 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG + chr3_1:74679107-74679126 Msa0375070:CDS 35.0%
ACTTTCATTGAAGGATCTTG+TGG + chr3_1:74679153-74679172 Msa0375070:CDS 35.0%
! ATTTCTGATGGACCAGTAAA+AGG + chr3_1:74679433-74679452 Msa0375070:CDS 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG + chr3_1:74679677-74679696 Msa0375070:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG + chr3_1:74679902-74679921 Msa0375070:intron 35.0%
AATCAAATCCCATGTTGGAT+TGG + chr3_1:74679907-74679926 Msa0375070:intron 35.0%
ATCAAATCCCATGTTGGATT+GGG + chr3_1:74679908-74679927 Msa0375070:intron 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG - chr3_1:74680094-74680113 None:intergenic 35.0%
! CTTTGTTTCATCAAAGGCAT+CGG - chr3_1:74680140-74680159 None:intergenic 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG - chr3_1:74680388-74680407 None:intergenic 35.0%
TCGTGATACATGTCCAATTG+AGG - chr3_1:74679001-74679020 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG + chr3_1:74679014-74679033 Msa0375070:CDS 40.0%
GAGAGGACAAAAAGAACTCA+TGG + chr3_1:74679096-74679115 Msa0375070:CDS 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG - chr3_1:74679186-74679205 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG + chr3_1:74679189-74679208 Msa0375070:CDS 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG - chr3_1:74679194-74679213 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG + chr3_1:74679198-74679217 Msa0375070:CDS 40.0%
AAAAAGGTAACCCCACTTTC+AGG - chr3_1:74679348-74679367 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG - chr3_1:74679424-74679443 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG + chr3_1:74679421-74679440 Msa0375070:CDS 40.0%
ACTGGTCCATCAGAAATTGA+TGG - chr3_1:74679430-74679449 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG - chr3_1:74679448-74679467 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG + chr3_1:74679447-74679466 Msa0375070:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG + chr3_1:74679458-74679477 Msa0375070:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG + chr3_1:74679461-74679480 Msa0375070:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG + chr3_1:74679611-74679630 Msa0375070:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG + chr3_1:74679676-74679695 Msa0375070:intron 40.0%
TCGTGATACATGTCCAATTG+AGG - chr3_1:74679001-74679020 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG + chr3_1:74679014-74679033 Msa0375070:CDS 40.0%
GAGAGGACAAAAAGAACTCA+TGG + chr3_1:74679096-74679115 Msa0375070:CDS 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG - chr3_1:74679186-74679205 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG + chr3_1:74679189-74679208 Msa0375070:CDS 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG - chr3_1:74679194-74679213 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG + chr3_1:74679198-74679217 Msa0375070:CDS 40.0%
AAAAAGGTAACCCCACTTTC+AGG - chr3_1:74679348-74679367 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG - chr3_1:74679424-74679443 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG + chr3_1:74679421-74679440 Msa0375070:CDS 40.0%
ACTGGTCCATCAGAAATTGA+TGG - chr3_1:74679430-74679449 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG - chr3_1:74679448-74679467 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG + chr3_1:74679447-74679466 Msa0375070:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG + chr3_1:74679458-74679477 Msa0375070:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG + chr3_1:74679461-74679480 Msa0375070:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG + chr3_1:74679611-74679630 Msa0375070:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG + chr3_1:74679676-74679695 Msa0375070:intron 40.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG - chr3_1:74678893-74678912 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG - chr3_1:74678989-74679008 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG - chr3_1:74679066-74679085 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG + chr3_1:74679066-74679085 Msa0375070:CDS 45.0%
TGGCCTCTGTTCTTGAAACA+GGG - chr3_1:74679072-74679091 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG - chr3_1:74679073-74679092 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG + chr3_1:74679201-74679220 Msa0375070:CDS 45.0%
GGGAAAATTGATCGTGATCG+AGG + chr3_1:74679316-74679335 Msa0375070:CDS 45.0%
TCGAGGTTTCTACCTGAAAG+TGG + chr3_1:74679333-74679352 Msa0375070:CDS 45.0%
CGAGGTTTCTACCTGAAAGT+GGG + chr3_1:74679334-74679353 Msa0375070:CDS 45.0%
GAGGTTTCTACCTGAAAGTG+GGG + chr3_1:74679335-74679354 Msa0375070:CDS 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG + chr3_1:74679689-74679708 Msa0375070:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG + chr3_1:74679697-74679716 Msa0375070:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG - chr3_1:74679918-74679937 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG + chr3_1:74679929-74679948 Msa0375070:intron 45.0%
ATGAAATGCAGGCGTGAGAA+GGG + chr3_1:74680052-74680071 Msa0375070:intron 45.0%
TCATGTTTAATGGCAGGGCA+TGG + chr3_1:74680356-74680375 Msa0375070:three_prime_UTR 45.0%
CATGTTTAATGGCAGGGCAT+GGG + chr3_1:74680357-74680376 Msa0375070:three_prime_UTR 45.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG - chr3_1:74678893-74678912 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG - chr3_1:74678989-74679008 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG - chr3_1:74679066-74679085 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG + chr3_1:74679066-74679085 Msa0375070:CDS 45.0%
TGGCCTCTGTTCTTGAAACA+GGG - chr3_1:74679072-74679091 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG - chr3_1:74679073-74679092 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG + chr3_1:74679201-74679220 Msa0375070:CDS 45.0%
GGGAAAATTGATCGTGATCG+AGG + chr3_1:74679316-74679335 Msa0375070:CDS 45.0%
TCGAGGTTTCTACCTGAAAG+TGG + chr3_1:74679333-74679352 Msa0375070:CDS 45.0%
CGAGGTTTCTACCTGAAAGT+GGG + chr3_1:74679334-74679353 Msa0375070:CDS 45.0%
GAGGTTTCTACCTGAAAGTG+GGG + chr3_1:74679335-74679354 Msa0375070:CDS 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG + chr3_1:74679689-74679708 Msa0375070:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG + chr3_1:74679697-74679716 Msa0375070:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG - chr3_1:74679918-74679937 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG + chr3_1:74679929-74679948 Msa0375070:intron 45.0%
ATGAAATGCAGGCGTGAGAA+GGG + chr3_1:74680052-74680071 Msa0375070:intron 45.0%
TCATGTTTAATGGCAGGGCA+TGG + chr3_1:74680356-74680375 Msa0375070:three_prime_UTR 45.0%
CATGTTTAATGGCAGGGCAT+GGG + chr3_1:74680357-74680376 Msa0375070:three_prime_UTR 45.0%
GGCTTCCCATGTTTCATGTC+TGG - chr3_1:74678945-74678964 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG + chr3_1:74678985-74679004 Msa0375070:CDS 50.0%
GGTGAGTTCACACTCCATCT+AGG - chr3_1:74679031-74679050 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG - chr3_1:74679052-74679071 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG - chr3_1:74679071-74679090 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG + chr3_1:74679079-74679098 Msa0375070:CDS 50.0%
ATCTTGTGGAACACCACCAC+AGG + chr3_1:74679167-74679186 Msa0375070:CDS 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG - chr3_1:74679183-74679202 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG + chr3_1:74679529-74679548 Msa0375070:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG - chr3_1:74679919-74679938 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG + chr3_1:74679923-74679942 Msa0375070:intron 50.0%
GATGAAATGCAGGCGTGAGA+AGG + chr3_1:74680051-74680070 Msa0375070:intron 50.0%
! AGAAGGGTGGAGGTATATGC+TGG + chr3_1:74680068-74680087 Msa0375070:CDS 50.0%
GGCTTCCCATGTTTCATGTC+TGG - chr3_1:74678945-74678964 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG + chr3_1:74678985-74679004 Msa0375070:CDS 50.0%
GGTGAGTTCACACTCCATCT+AGG - chr3_1:74679031-74679050 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG - chr3_1:74679052-74679071 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG - chr3_1:74679071-74679090 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG + chr3_1:74679079-74679098 Msa0375070:CDS 50.0%
ATCTTGTGGAACACCACCAC+AGG + chr3_1:74679167-74679186 Msa0375070:CDS 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG - chr3_1:74679183-74679202 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG + chr3_1:74679529-74679548 Msa0375070:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG - chr3_1:74679919-74679938 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG + chr3_1:74679923-74679942 Msa0375070:intron 50.0%
GATGAAATGCAGGCGTGAGA+AGG + chr3_1:74680051-74680070 Msa0375070:intron 50.0%
! AGAAGGGTGGAGGTATATGC+TGG + chr3_1:74680068-74680087 Msa0375070:CDS 50.0%
TACAGAGGAGCAGAAGGAGG+AGG + chr3_1:74679204-74679223 Msa0375070:CDS 55.0%
AAATGCAGGCGTGAGAAGGG+TGG + chr3_1:74680055-74680074 Msa0375070:intron 55.0%
GTTTAATGGCAGGGCATGGG+AGG + chr3_1:74680360-74680379 Msa0375070:three_prime_UTR 55.0%
TACAGAGGAGCAGAAGGAGG+AGG + chr3_1:74679204-74679223 Msa0375070:CDS 55.0%
AAATGCAGGCGTGAGAAGGG+TGG + chr3_1:74680055-74680074 Msa0375070:intron 55.0%
GTTTAATGGCAGGGCATGGG+AGG + chr3_1:74680360-74680379 Msa0375070:three_prime_UTR 55.0%
AGAGGAGCAGAAGGAGGAGG+AGG + chr3_1:74679207-74679226 Msa0375070:CDS 60.0%
GCAGAAGGAGGAGGAGGAGA+AGG + chr3_1:74679213-74679232 Msa0375070:CDS 60.0%
GGAGGAGGAGGAGAAGGAGA+AGG + chr3_1:74679219-74679238 Msa0375070:CDS 60.0%
AGAGGAGCAGAAGGAGGAGG+AGG + chr3_1:74679207-74679226 Msa0375070:CDS 60.0%
GCAGAAGGAGGAGGAGGAGA+AGG + chr3_1:74679213-74679232 Msa0375070:CDS 60.0%
GGAGGAGGAGGAGAAGGAGA+AGG + chr3_1:74679219-74679238 Msa0375070:CDS 60.0%
TGCAGGCGTGAGAAGGGTGG+AGG + chr3_1:74680058-74680077 Msa0375070:intron 65.0%
TGCAGGCGTGAGAAGGGTGG+AGG + chr3_1:74680058-74680077 Msa0375070:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 74678881 74680442 74678881 ID=Msa0375070;Name=Msa0375070
chr3_1 mRNA 74678881 74680442 74678881 ID=Msa0375070-mRNA-1;Parent=Msa0375070;Name=Msa0375070-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0.5|1|1|1|2|321|210
chr3_1 exon 74678881 74679454 74678881 ID=Msa0375070-mRNA-1:exon:17345;Parent=Msa0375070-mRNA-1
chr3_1 exon 74680063 74680442 74680063 ID=Msa0375070-mRNA-1:exon:17346;Parent=Msa0375070-mRNA-1
chr3_1 CDS 74678881 74679454 74678881 ID=Msa0375070-mRNA-1:cds;Parent=Msa0375070-mRNA-1
chr3_1 CDS 74680063 74680121 74680063 ID=Msa0375070-mRNA-1:cds;Parent=Msa0375070-mRNA-1
chr3_1 three_prime_UTR 74680122 74680442 74680122 ID=Msa0375070-mRNA-1:three_prime_utr;Parent=Msa0375070-mRNA-1
Gene Sequence

>Msa0375070

ATGCTTGATCCAACAAGAAAACACCACCAACCTAAGAATTCTTTTGATTTGTACGATGAAACCAGACATGAAACATGGGAAGCCACAAATTCTAATAATCTCTGGCCTTCAACACAGCCTCAATTGGACATGTATCACGATGAACAACCTAGATGGAGTGTGAACTCACCACATTACAGAAGCCTTTCCCCTGTTTCAAGAACAGAGGCCATAGTGAGAGGACAAAAAGAACTCATGGAAATGGTTCGGAACATGCCCGAATCAAACTATGAACTTTCATTGAAGGATCTTGTGGAACACCACCACAGGACCAGAGAAGAAAATACAGAGGAGCAGAAGGAGGAGGAGGAGAAGGAGAAGGAGAAGAAGAATTCTAGTGTCTATAATGGTGGCAAGAAGATTGATAATAAGAAGGTACATGTGAAGAAAAATGATGGGAAAATTGATCGTGATCGAGGTTTCTACCTGAAAGTGGGGTTACCTTTTTTTTCTTTAGGATCTAAAGAGAAAAAGAAGAAGAAAAAGGAGTCAAAAGTTTCACCAAGACCATCAATTTCTGATGGACCAGTAAAAGGCGTGAGAAGGGTGGAGGTATATGCTGGCCTTTGTTTCGCAAACCCAAAATCCAAATAA
Protein sequence

>Msa0375070

MLDPTRKHHQPKNSFDLYDETRHETWEATNSNNLWPSTQPQLDMYHDEQPRWSVNSPHYRSLSPVSRTEAIVRGQKELMEMVRNMPESNYELSLKDLVEHHHRTREENTEEQKEEEEKEKEKKNSSVYNGGKKIDNKKVHVKKNDGKIDRDRGFYLKVGLPFFSLGSKEKKKKKKESKVSPRPSISDGPVKGVRRVEVYAGLCFANPKSK*