AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0374890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374890 MtrunA17_Chr3g0125561 84.536 194 18 3 1 193 31 213 7.90e-93 273
Msa0374890 MtrunA17_Chr1g0159571 44.444 207 82 9 1 190 1 191 3.51e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374890 AT1G21390.1 43.537 147 54 5 59 191 52 183 1.39e-17 79.0
Msa0374890 AT1G76980.1 41.463 164 62 7 50 191 33 184 3.35e-17 77.8
Msa0374890 AT1G76980.2 41.463 164 62 7 50 191 33 184 4.55e-17 77.4

Find 62 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATCATCATGTTTAA+TGG 0.197990 3_1:+74427043 Msa0374890:three_prime_UTR
GAATTAGATAACTTTGCTTC+TGG 0.283440 3_1:+74426987 Msa0374890:three_prime_UTR
ATTTCTGATGGACCAGTAAA+AGG 0.323657 3_1:+74426129 Msa0374890:CDS
ATGGCCTCTGTTCTTGAAAC+AGG 0.336109 3_1:-74425766 None:intergenic
TACAAATCAAAAGAATTCTT+AGG 0.338959 3_1:-74425607 None:intergenic
GAAAGTTCATAGTTTGATTC+GGG 0.347032 3_1:-74425832 None:intergenic
CCAGAGATTATTAGAATTTG+TGG 0.349920 3_1:-74425659 None:intergenic
TGAAAGTTCATAGTTTGATT+CGG 0.368860 3_1:-74425833 None:intergenic
TTCTTTATATGTTTATTTGA+AGG 0.375267 3_1:+74427119 Msa0374890:three_prime_UTR
GCAGAAGGAGGAGGAGGAGA+AGG 0.389816 3_1:+74425909 Msa0374890:CDS
GCCTTCAACACAGCCTCAAT+TGG 0.391567 3_1:+74425681 Msa0374890:CDS
GGCTTCCCATGTTTCATGTC+TGG 0.393616 3_1:-74425638 None:intergenic
AAACTATGAACTTTCATTGA+AGG 0.394782 3_1:+74425840 Msa0374890:CDS
ACTGGTCCATCAGAAATTGA+TGG 0.402064 3_1:-74426123 None:intergenic
AAGAATTCTAGTGTCTATAA+TGG 0.423589 3_1:+74425943 Msa0374890:CDS
ATCACGATGAACAACCTAGA+TGG 0.455248 3_1:+74425710 Msa0374890:CDS
AGCAAAGTTATCTAATTCAA+TGG 0.455720 3_1:-74426982 None:intergenic
ATATCATCATGTTTAATGGC+AGG 0.462367 3_1:+74427047 Msa0374890:three_prime_UTR
CCACAAATTCTAATAATCTC+TGG 0.468437 3_1:+74425659 Msa0374890:CDS
GGAGGAGGAGGAGAAGGAGA+AGG 0.482742 3_1:+74425915 Msa0374890:CDS
AAGAACTCATGGAAATGGTT+CGG 0.483414 3_1:+74425803 Msa0374890:CDS
AGAAAATACAGAGGAGCAGA+AGG 0.490882 3_1:+74425894 Msa0374890:CDS
TCATGTTTAATGGCAGGGCA+TGG 0.506028 3_1:+74427053 Msa0374890:three_prime_UTR
AGAGGAGCAGAAGGAGGAGG+AGG 0.512087 3_1:+74425903 Msa0374890:CDS
CCATCAGAAATTGATGGTCT+TGG 0.516641 3_1:-74426117 None:intergenic
TCCAATTGAGGCTGTGTTGA+AGG 0.516902 3_1:-74425682 None:intergenic
GGTGAGTTCACACTCCATCT+AGG 0.517049 3_1:-74425724 None:intergenic
ATCTTGTGGAACACCACCAC+AGG 0.517846 3_1:+74425863 Msa0374890:CDS
ATAGTACTTTGTTTCATCAA+AGG 0.520728 3_1:-74426840 None:intergenic
ACTTTCATTGAAGGATCTTG+TGG 0.526030 3_1:+74425849 Msa0374890:CDS
CTTTGTTTCATCAAAGGCAT+CGG 0.528423 3_1:-74426834 None:intergenic
TTACAAAGTTTGGATTGGCA+TGG 0.529260 3_1:-74427082 None:intergenic
TACAGAGGAGCAGAAGGAGG+AGG 0.532433 3_1:+74425900 Msa0374890:CDS
AATCAAAAGAATTCTTAGGT+TGG 0.534469 3_1:-74425603 None:intergenic
CCAAGACCATCAATTTCTGA+TGG 0.535937 3_1:+74426117 Msa0374890:CDS
CAAAAGAATTCTTAGGTTGG+TGG 0.537203 3_1:-74425600 None:intergenic
CGAGGTTTCTACCTGAAAGT+GGG 0.539143 3_1:+74426030 Msa0374890:CDS
TTTCTTCTCTGGTCCTGTGG+TGG 0.548885 3_1:-74425876 None:intergenic
CATGTTTAATGGCAGGGCAT+GGG 0.551016 3_1:+74427054 Msa0374890:three_prime_UTR
CAAGAAGATTGATAATAAGA+AGG 0.553116 3_1:+74425969 Msa0374890:CDS
GGGAAAATTGATCGTGATCG+AGG 0.558249 3_1:+74426012 Msa0374890:CDS
CTGTTCTTGAAACAGGGGAA+AGG 0.560820 3_1:-74425759 None:intergenic
AGAAGGGTGGAGGTATATGC+TGG 0.561488 3_1:+74426765 Msa0374890:CDS
TGCAGGCGTGAGAAGGGTGG+AGG 0.562031 3_1:+74426755 Msa0374890:intron
TCGAGGTTTCTACCTGAAAG+TGG 0.562734 3_1:+74426029 Msa0374890:CDS
TCGTGATACATGTCCAATTG+AGG 0.569340 3_1:-74425694 None:intergenic
GTTTAATGGCAGGGCATGGG+AGG 0.571187 3_1:+74427057 Msa0374890:three_prime_UTR
TATCATCATGTTTAATGGCA+GGG 0.575049 3_1:+74427048 Msa0374890:three_prime_UTR
TGGCCTCTGTTCTTGAAACA+GGG 0.597218 3_1:-74425765 None:intergenic
ATGAAACCAGACATGAAACA+TGG 0.602607 3_1:+74425632 Msa0374890:CDS
AGAACAGAGGCCATAGTGAG+AGG 0.606015 3_1:+74425775 Msa0374890:CDS
AAATGCAGGCGTGAGAAGGG+TGG 0.606501 3_1:+74426752 Msa0374890:intron
AATTCTAGTGTCTATAATGG+TGG 0.616757 3_1:+74425946 Msa0374890:CDS
TTCCCCTGTTTCAAGAACAG+AGG 0.619257 3_1:+74425762 Msa0374890:CDS
GGGGAAAGGCTTCTGTAATG+TGG 0.623224 3_1:-74425745 None:intergenic
AAATACAGAGGAGCAGAAGG+AGG 0.627616 3_1:+74425897 Msa0374890:CDS
GATGAAATGCAGGCGTGAGA+AGG 0.636676 3_1:+74426748 Msa0374890:intron
ATGAAATGCAGGCGTGAGAA+GGG 0.643514 3_1:+74426749 Msa0374890:intron
GGCCTCTGTTCTTGAAACAG+GGG 0.643547 3_1:-74425764 None:intergenic
GAGGTTTCTACCTGAAAGTG+GGG 0.662564 3_1:+74426031 Msa0374890:CDS
TGAAACCAGACATGAAACAT+GGG 0.668487 3_1:+74425633 Msa0374890:CDS
GACCAGAGAAGAAAATACAG+AGG 0.687498 3_1:+74425885 Msa0374890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTAATTAATTTGAAGTT+GGG - chr3_1:74426342-74426361 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG - chr3_1:74426343-74426362 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG + chr3_1:74426472-74426491 Msa0374890:intron 15.0%
!!! AAGAAAATAACTCTTTTTAT+TGG + chr3_1:74426656-74426675 Msa0374890:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG - chr3_1:74426806-74426825 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG - chr3_1:74426807-74426826 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG - chr3_1:74426814-74426833 None:intergenic 15.0%
!!! ATGTTAATTAATTTGAAGTT+GGG - chr3_1:74426342-74426361 None:intergenic 15.0%
!!! TATGTTAATTAATTTGAAGT+TGG - chr3_1:74426343-74426362 None:intergenic 15.0%
!!! TTCAAATTTATTTGTTTCTT+AGG + chr3_1:74426472-74426491 Msa0374890:intron 15.0%
!!! AAGAAAATAACTCTTTTTAT+TGG + chr3_1:74426656-74426675 Msa0374890:intron 15.0%
!!! TACTTTTTTATTTGGATTTT+GGG - chr3_1:74426806-74426825 None:intergenic 15.0%
!!! TTACTTTTTTATTTGGATTT+TGG - chr3_1:74426807-74426826 None:intergenic 15.0%
!!! TGATCTTTTACTTTTTTATT+TGG - chr3_1:74426814-74426833 None:intergenic 15.0%
!! TACAAATCAAAAGAATTCTT+AGG - chr3_1:74425610-74425629 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG - chr3_1:74426060-74426079 None:intergenic 20.0%
!! TAACAAAAATTTCATGTTCA+TGG + chr3_1:74426540-74426559 Msa0374890:intron 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG + chr3_1:74426699-74426718 Msa0374890:intron 20.0%
!! AAAAAAAAATTGTTCTCAGA+TGG - chr3_1:74426926-74426945 None:intergenic 20.0%
!! ACAAATATCATCATGTTTAA+TGG + chr3_1:74427043-74427062 Msa0374890:three_prime_UTR 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG - chr3_1:74427090-74427109 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG - chr3_1:74427095-74427114 None:intergenic 20.0%
!! TACAAATCAAAAGAATTCTT+AGG - chr3_1:74425610-74425629 None:intergenic 20.0%
!! TTAGATCCTAAAGAAAAAAA+AGG - chr3_1:74426060-74426079 None:intergenic 20.0%
!! TAACAAAAATTTCATGTTCA+TGG + chr3_1:74426540-74426559 Msa0374890:intron 20.0%
!!! TGTCTTTTTGCTTATATTTA+AGG + chr3_1:74426699-74426718 Msa0374890:intron 20.0%
!! AAAAAAAAATTGTTCTCAGA+TGG - chr3_1:74426926-74426945 None:intergenic 20.0%
!! ACAAATATCATCATGTTTAA+TGG + chr3_1:74427043-74427062 Msa0374890:three_prime_UTR 20.0%
!! AAAAATTACAAAGTTTGGAT+TGG - chr3_1:74427090-74427109 None:intergenic 20.0%
!! ATGTCAAAAATTACAAAGTT+TGG - chr3_1:74427095-74427114 None:intergenic 20.0%
! AATCAAAAGAATTCTTAGGT+TGG - chr3_1:74425606-74425625 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG - chr3_1:74425836-74425855 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG + chr3_1:74425840-74425859 Msa0374890:CDS 25.0%
! AAGAATTCTAGTGTCTATAA+TGG + chr3_1:74425943-74425962 Msa0374890:CDS 25.0%
!! CAAGAAGATTGATAATAAGA+AGG + chr3_1:74425969-74425988 Msa0374890:CDS 25.0%
! TACATGTGAAGAAAAATGAT+GGG + chr3_1:74425992-74426011 Msa0374890:CDS 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG + chr3_1:74426080-74426099 Msa0374890:CDS 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG + chr3_1:74426365-74426384 Msa0374890:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG + chr3_1:74426574-74426593 Msa0374890:intron 25.0%
! TAAGCAAAAAGACATGTATT+TGG - chr3_1:74426693-74426712 None:intergenic 25.0%
!!! ATAGAGTACTGATTAACTTT+TGG + chr3_1:74426726-74426745 Msa0374890:intron 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG - chr3_1:74426843-74426862 None:intergenic 25.0%
! AGCAAAGTTATCTAATTCAA+TGG - chr3_1:74426985-74427004 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG + chr3_1:74427009-74427028 Msa0374890:three_prime_UTR 25.0%
! AATCAAAAGAATTCTTAGGT+TGG - chr3_1:74425606-74425625 None:intergenic 25.0%
! TGAAAGTTCATAGTTTGATT+CGG - chr3_1:74425836-74425855 None:intergenic 25.0%
! AAACTATGAACTTTCATTGA+AGG + chr3_1:74425840-74425859 Msa0374890:CDS 25.0%
! AAGAATTCTAGTGTCTATAA+TGG + chr3_1:74425943-74425962 Msa0374890:CDS 25.0%
!! CAAGAAGATTGATAATAAGA+AGG + chr3_1:74425969-74425988 Msa0374890:CDS 25.0%
! TACATGTGAAGAAAAATGAT+GGG + chr3_1:74425992-74426011 Msa0374890:CDS 25.0%
! AGAGAAAAAGAAGAAGAAAA+AGG + chr3_1:74426080-74426099 Msa0374890:CDS 25.0%
!!! ATGTATAGTTGATTAGTTTC+TGG + chr3_1:74426365-74426384 Msa0374890:intron 25.0%
!! ACATTTTTATGCTTCTTTAG+AGG + chr3_1:74426574-74426593 Msa0374890:intron 25.0%
! TAAGCAAAAAGACATGTATT+TGG - chr3_1:74426693-74426712 None:intergenic 25.0%
!!! ATAGAGTACTGATTAACTTT+TGG + chr3_1:74426726-74426745 Msa0374890:intron 25.0%
!! ATAGTACTTTGTTTCATCAA+AGG - chr3_1:74426843-74426862 None:intergenic 25.0%
! AGCAAAGTTATCTAATTCAA+TGG - chr3_1:74426985-74427004 None:intergenic 25.0%
! GAGTTACTAAAAAATGTGTA+AGG + chr3_1:74427009-74427028 Msa0374890:three_prime_UTR 25.0%
CCAGAGATTATTAGAATTTG+TGG - chr3_1:74425662-74425681 None:intergenic 30.0%
CCACAAATTCTAATAATCTC+TGG + chr3_1:74425659-74425678 Msa0374890:CDS 30.0%
ACAAAAAGAACTCATGGAAA+TGG + chr3_1:74425798-74425817 Msa0374890:CDS 30.0%
GAAAGTTCATAGTTTGATTC+GGG - chr3_1:74425835-74425854 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG + chr3_1:74425946-74425965 Msa0374890:CDS 30.0%
GTACATGTGAAGAAAAATGA+TGG + chr3_1:74425991-74426010 Msa0374890:CDS 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG + chr3_1:74426051-74426070 Msa0374890:CDS 30.0%
TCACTCTCTTTATAAACAGA+AGG - chr3_1:74426192-74426211 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG - chr3_1:74426404-74426423 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG + chr3_1:74426547-74426566 Msa0374890:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG - chr3_1:74426635-74426654 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG - chr3_1:74426636-74426655 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG + chr3_1:74426738-74426757 Msa0374890:intron 30.0%
GAATTAGATAACTTTGCTTC+TGG + chr3_1:74426987-74427006 Msa0374890:three_prime_UTR 30.0%
ATATCATCATGTTTAATGGC+AGG + chr3_1:74427047-74427066 Msa0374890:three_prime_UTR 30.0%
TATCATCATGTTTAATGGCA+GGG + chr3_1:74427048-74427067 Msa0374890:three_prime_UTR 30.0%
CCAGAGATTATTAGAATTTG+TGG - chr3_1:74425662-74425681 None:intergenic 30.0%
CCACAAATTCTAATAATCTC+TGG + chr3_1:74425659-74425678 Msa0374890:CDS 30.0%
ACAAAAAGAACTCATGGAAA+TGG + chr3_1:74425798-74425817 Msa0374890:CDS 30.0%
GAAAGTTCATAGTTTGATTC+GGG - chr3_1:74425835-74425854 None:intergenic 30.0%
AATTCTAGTGTCTATAATGG+TGG + chr3_1:74425946-74425965 Msa0374890:CDS 30.0%
GTACATGTGAAGAAAAATGA+TGG + chr3_1:74425991-74426010 Msa0374890:CDS 30.0%
!!! GGGTTACCTTTTTTTTCTTT+AGG + chr3_1:74426051-74426070 Msa0374890:CDS 30.0%
TCACTCTCTTTATAAACAGA+AGG - chr3_1:74426192-74426211 None:intergenic 30.0%
AATTTAAACCAAACAGCCAA+AGG - chr3_1:74426404-74426423 None:intergenic 30.0%
AATTTCATGTTCATGGTTGT+TGG + chr3_1:74426547-74426566 Msa0374890:intron 30.0%
AAATTCTCCAAATCCAACAT+GGG - chr3_1:74426635-74426654 None:intergenic 30.0%
AAAATTCTCCAAATCCAACA+TGG - chr3_1:74426636-74426655 None:intergenic 30.0%
! TTAACTTTTGGATGAAATGC+AGG + chr3_1:74426738-74426757 Msa0374890:intron 30.0%
GAATTAGATAACTTTGCTTC+TGG + chr3_1:74426987-74427006 Msa0374890:three_prime_UTR 30.0%
ATATCATCATGTTTAATGGC+AGG + chr3_1:74427047-74427066 Msa0374890:three_prime_UTR 30.0%
TATCATCATGTTTAATGGCA+GGG + chr3_1:74427048-74427067 Msa0374890:three_prime_UTR 30.0%
!! CAAAAGAATTCTTAGGTTGG+TGG - chr3_1:74425603-74425622 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG + chr3_1:74425632-74425651 Msa0374890:CDS 35.0%
TGAAACCAGACATGAAACAT+GGG + chr3_1:74425633-74425652 Msa0374890:CDS 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG - chr3_1:74425788-74425807 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG + chr3_1:74425803-74425822 Msa0374890:CDS 35.0%
ACTTTCATTGAAGGATCTTG+TGG + chr3_1:74425849-74425868 Msa0374890:CDS 35.0%
! ATTTCTGATGGACCAGTAAA+AGG + chr3_1:74426129-74426148 Msa0374890:CDS 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG + chr3_1:74426373-74426392 Msa0374890:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG + chr3_1:74426598-74426617 Msa0374890:intron 35.0%
AATCAAATCCCATGTTGGAT+TGG + chr3_1:74426603-74426622 Msa0374890:intron 35.0%
ATCAAATCCCATGTTGGATT+GGG + chr3_1:74426604-74426623 Msa0374890:intron 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG - chr3_1:74426791-74426810 None:intergenic 35.0%
! CTTTGTTTCATCAAAGGCAT+CGG - chr3_1:74426837-74426856 None:intergenic 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG - chr3_1:74427085-74427104 None:intergenic 35.0%
!! CAAAAGAATTCTTAGGTTGG+TGG - chr3_1:74425603-74425622 None:intergenic 35.0%
ATGAAACCAGACATGAAACA+TGG + chr3_1:74425632-74425651 Msa0374890:CDS 35.0%
TGAAACCAGACATGAAACAT+GGG + chr3_1:74425633-74425652 Msa0374890:CDS 35.0%
! TTCTTTTTGTCCTCTCACTA+TGG - chr3_1:74425788-74425807 None:intergenic 35.0%
AAGAACTCATGGAAATGGTT+CGG + chr3_1:74425803-74425822 Msa0374890:CDS 35.0%
ACTTTCATTGAAGGATCTTG+TGG + chr3_1:74425849-74425868 Msa0374890:CDS 35.0%
! ATTTCTGATGGACCAGTAAA+AGG + chr3_1:74426129-74426148 Msa0374890:CDS 35.0%
!! TTGATTAGTTTCTGGCTAGT+GGG + chr3_1:74426373-74426392 Msa0374890:intron 35.0%
GTTCAAATCAAATCCCATGT+TGG + chr3_1:74426598-74426617 Msa0374890:intron 35.0%
AATCAAATCCCATGTTGGAT+TGG + chr3_1:74426603-74426622 Msa0374890:intron 35.0%
ATCAAATCCCATGTTGGATT+GGG + chr3_1:74426604-74426623 Msa0374890:intron 35.0%
!!! ATTTTGGGTTTGCGAAACAA+AGG - chr3_1:74426791-74426810 None:intergenic 35.0%
! CTTTGTTTCATCAAAGGCAT+CGG - chr3_1:74426837-74426856 None:intergenic 35.0%
!! TTACAAAGTTTGGATTGGCA+TGG - chr3_1:74427085-74427104 None:intergenic 35.0%
TCGTGATACATGTCCAATTG+AGG - chr3_1:74425697-74425716 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG + chr3_1:74425710-74425729 Msa0374890:CDS 40.0%
GAGAGGACAAAAAGAACTCA+TGG + chr3_1:74425792-74425811 Msa0374890:CDS 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG - chr3_1:74425882-74425901 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG + chr3_1:74425885-74425904 Msa0374890:CDS 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG - chr3_1:74425890-74425909 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG + chr3_1:74425894-74425913 Msa0374890:CDS 40.0%
AAAAAGGTAACCCCACTTTC+AGG - chr3_1:74426044-74426063 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG - chr3_1:74426120-74426139 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG + chr3_1:74426117-74426136 Msa0374890:CDS 40.0%
ACTGGTCCATCAGAAATTGA+TGG - chr3_1:74426126-74426145 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG - chr3_1:74426144-74426163 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG + chr3_1:74426143-74426162 Msa0374890:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG + chr3_1:74426154-74426173 Msa0374890:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG + chr3_1:74426157-74426176 Msa0374890:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG + chr3_1:74426307-74426326 Msa0374890:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG + chr3_1:74426372-74426391 Msa0374890:intron 40.0%
TCGTGATACATGTCCAATTG+AGG - chr3_1:74425697-74425716 None:intergenic 40.0%
ATCACGATGAACAACCTAGA+TGG + chr3_1:74425710-74425729 Msa0374890:CDS 40.0%
GAGAGGACAAAAAGAACTCA+TGG + chr3_1:74425792-74425811 Msa0374890:CDS 40.0%
! TATTTTCTTCTCTGGTCCTG+TGG - chr3_1:74425882-74425901 None:intergenic 40.0%
GACCAGAGAAGAAAATACAG+AGG + chr3_1:74425885-74425904 Msa0374890:CDS 40.0%
! CTCCTCTGTATTTTCTTCTC+TGG - chr3_1:74425890-74425909 None:intergenic 40.0%
AGAAAATACAGAGGAGCAGA+AGG + chr3_1:74425894-74425913 Msa0374890:CDS 40.0%
AAAAAGGTAACCCCACTTTC+AGG - chr3_1:74426044-74426063 None:intergenic 40.0%
! CCATCAGAAATTGATGGTCT+TGG - chr3_1:74426120-74426139 None:intergenic 40.0%
CCAAGACCATCAATTTCTGA+TGG + chr3_1:74426117-74426136 Msa0374890:CDS 40.0%
ACTGGTCCATCAGAAATTGA+TGG - chr3_1:74426126-74426145 None:intergenic 40.0%
! TTATCAGCAGCACCTTTTAC+TGG - chr3_1:74426144-74426163 None:intergenic 40.0%
!! AGTAAAAGGTGCTGCTGATA+AGG + chr3_1:74426143-74426162 Msa0374890:intron 40.0%
CTGCTGATAAGGAAAAAGAG+TGG + chr3_1:74426154-74426173 Msa0374890:intron 40.0%
CTGATAAGGAAAAAGAGTGG+TGG + chr3_1:74426157-74426176 Msa0374890:intron 40.0%
GACTAAGAAGCAATAGCATC+AGG + chr3_1:74426307-74426326 Msa0374890:intron 40.0%
!! GTTGATTAGTTTCTGGCTAG+TGG + chr3_1:74426372-74426391 Msa0374890:intron 40.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG - chr3_1:74425589-74425608 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG - chr3_1:74425685-74425704 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG - chr3_1:74425762-74425781 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG + chr3_1:74425762-74425781 Msa0374890:CDS 45.0%
TGGCCTCTGTTCTTGAAACA+GGG - chr3_1:74425768-74425787 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG - chr3_1:74425769-74425788 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG + chr3_1:74425897-74425916 Msa0374890:CDS 45.0%
GGGAAAATTGATCGTGATCG+AGG + chr3_1:74426012-74426031 Msa0374890:CDS 45.0%
TCGAGGTTTCTACCTGAAAG+TGG + chr3_1:74426029-74426048 Msa0374890:CDS 45.0%
CGAGGTTTCTACCTGAAAGT+GGG + chr3_1:74426030-74426049 Msa0374890:CDS 45.0%
GAGGTTTCTACCTGAAAGTG+GGG + chr3_1:74426031-74426050 Msa0374890:CDS 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG + chr3_1:74426385-74426404 Msa0374890:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG + chr3_1:74426393-74426412 Msa0374890:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG - chr3_1:74426614-74426633 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG + chr3_1:74426625-74426644 Msa0374890:intron 45.0%
ATGAAATGCAGGCGTGAGAA+GGG + chr3_1:74426749-74426768 Msa0374890:intron 45.0%
TCATGTTTAATGGCAGGGCA+TGG + chr3_1:74427053-74427072 Msa0374890:three_prime_UTR 45.0%
CATGTTTAATGGCAGGGCAT+GGG + chr3_1:74427054-74427073 Msa0374890:three_prime_UTR 45.0%
!!! GGTTGGTGGTGTTTTCTTGT+TGG - chr3_1:74425589-74425608 None:intergenic 45.0%
TCCAATTGAGGCTGTGTTGA+AGG - chr3_1:74425685-74425704 None:intergenic 45.0%
CTGTTCTTGAAACAGGGGAA+AGG - chr3_1:74425762-74425781 None:intergenic 45.0%
TTCCCCTGTTTCAAGAACAG+AGG + chr3_1:74425762-74425781 Msa0374890:CDS 45.0%
TGGCCTCTGTTCTTGAAACA+GGG - chr3_1:74425768-74425787 None:intergenic 45.0%
ATGGCCTCTGTTCTTGAAAC+AGG - chr3_1:74425769-74425788 None:intergenic 45.0%
AAATACAGAGGAGCAGAAGG+AGG + chr3_1:74425897-74425916 Msa0374890:CDS 45.0%
GGGAAAATTGATCGTGATCG+AGG + chr3_1:74426012-74426031 Msa0374890:CDS 45.0%
TCGAGGTTTCTACCTGAAAG+TGG + chr3_1:74426029-74426048 Msa0374890:CDS 45.0%
CGAGGTTTCTACCTGAAAGT+GGG + chr3_1:74426030-74426049 Msa0374890:CDS 45.0%
GAGGTTTCTACCTGAAAGTG+GGG + chr3_1:74426031-74426050 Msa0374890:CDS 45.0%
!!! TGGCTAGTGGGTTTTTCCTT+TGG + chr3_1:74426385-74426404 Msa0374890:intron 45.0%
! GGGTTTTTCCTTTGGCTGTT+TGG + chr3_1:74426393-74426412 Msa0374890:intron 45.0%
GGATTCTCCCAATCCAACAT+GGG - chr3_1:74426614-74426633 None:intergenic 45.0%
GGAGAATCCCATGTTGGATT+TGG + chr3_1:74426625-74426644 Msa0374890:intron 45.0%
ATGAAATGCAGGCGTGAGAA+GGG + chr3_1:74426749-74426768 Msa0374890:intron 45.0%
TCATGTTTAATGGCAGGGCA+TGG + chr3_1:74427053-74427072 Msa0374890:three_prime_UTR 45.0%
CATGTTTAATGGCAGGGCAT+GGG + chr3_1:74427054-74427073 Msa0374890:three_prime_UTR 45.0%
GGCTTCCCATGTTTCATGTC+TGG - chr3_1:74425641-74425660 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG + chr3_1:74425681-74425700 Msa0374890:CDS 50.0%
GGTGAGTTCACACTCCATCT+AGG - chr3_1:74425727-74425746 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG - chr3_1:74425748-74425767 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG - chr3_1:74425767-74425786 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG + chr3_1:74425775-74425794 Msa0374890:CDS 50.0%
ATCTTGTGGAACACCACCAC+AGG + chr3_1:74425863-74425882 Msa0374890:CDS 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG - chr3_1:74425879-74425898 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG + chr3_1:74426225-74426244 Msa0374890:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG - chr3_1:74426615-74426634 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG + chr3_1:74426619-74426638 Msa0374890:intron 50.0%
GATGAAATGCAGGCGTGAGA+AGG + chr3_1:74426748-74426767 Msa0374890:intron 50.0%
! AGAAGGGTGGAGGTATATGC+TGG + chr3_1:74426765-74426784 Msa0374890:CDS 50.0%
GGCTTCCCATGTTTCATGTC+TGG - chr3_1:74425641-74425660 None:intergenic 50.0%
GCCTTCAACACAGCCTCAAT+TGG + chr3_1:74425681-74425700 Msa0374890:CDS 50.0%
GGTGAGTTCACACTCCATCT+AGG - chr3_1:74425727-74425746 None:intergenic 50.0%
GGGGAAAGGCTTCTGTAATG+TGG - chr3_1:74425748-74425767 None:intergenic 50.0%
GGCCTCTGTTCTTGAAACAG+GGG - chr3_1:74425767-74425786 None:intergenic 50.0%
AGAACAGAGGCCATAGTGAG+AGG + chr3_1:74425775-74425794 Msa0374890:CDS 50.0%
ATCTTGTGGAACACCACCAC+AGG + chr3_1:74425863-74425882 Msa0374890:CDS 50.0%
TTTCTTCTCTGGTCCTGTGG+TGG - chr3_1:74425879-74425898 None:intergenic 50.0%
GCATCGAGCAGCAACAGTAA+TGG + chr3_1:74426225-74426244 Msa0374890:intron 50.0%
GGGATTCTCCCAATCCAACA+TGG - chr3_1:74426615-74426634 None:intergenic 50.0%
GGATTGGGAGAATCCCATGT+TGG + chr3_1:74426619-74426638 Msa0374890:intron 50.0%
GATGAAATGCAGGCGTGAGA+AGG + chr3_1:74426748-74426767 Msa0374890:intron 50.0%
! AGAAGGGTGGAGGTATATGC+TGG + chr3_1:74426765-74426784 Msa0374890:CDS 50.0%
TACAGAGGAGCAGAAGGAGG+AGG + chr3_1:74425900-74425919 Msa0374890:CDS 55.0%
AAATGCAGGCGTGAGAAGGG+TGG + chr3_1:74426752-74426771 Msa0374890:intron 55.0%
GTTTAATGGCAGGGCATGGG+AGG + chr3_1:74427057-74427076 Msa0374890:three_prime_UTR 55.0%
TACAGAGGAGCAGAAGGAGG+AGG + chr3_1:74425900-74425919 Msa0374890:CDS 55.0%
AAATGCAGGCGTGAGAAGGG+TGG + chr3_1:74426752-74426771 Msa0374890:intron 55.0%
GTTTAATGGCAGGGCATGGG+AGG + chr3_1:74427057-74427076 Msa0374890:three_prime_UTR 55.0%
AGAGGAGCAGAAGGAGGAGG+AGG + chr3_1:74425903-74425922 Msa0374890:CDS 60.0%
GCAGAAGGAGGAGGAGGAGA+AGG + chr3_1:74425909-74425928 Msa0374890:CDS 60.0%
GGAGGAGGAGGAGAAGGAGA+AGG + chr3_1:74425915-74425934 Msa0374890:CDS 60.0%
AGAGGAGCAGAAGGAGGAGG+AGG + chr3_1:74425903-74425922 Msa0374890:CDS 60.0%
GCAGAAGGAGGAGGAGGAGA+AGG + chr3_1:74425909-74425928 Msa0374890:CDS 60.0%
GGAGGAGGAGGAGAAGGAGA+AGG + chr3_1:74425915-74425934 Msa0374890:CDS 60.0%
TGCAGGCGTGAGAAGGGTGG+AGG + chr3_1:74426755-74426774 Msa0374890:intron 65.0%
TGCAGGCGTGAGAAGGGTGG+AGG + chr3_1:74426755-74426774 Msa0374890:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 74425577 74427139 74425577 ID=Msa0374890;Name=Msa0374890
chr3_1 mRNA 74425577 74427139 74425577 ID=Msa0374890-mRNA-1;Parent=Msa0374890;Name=Msa0374890-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0.5|1|0|0.5|2|321|210
chr3_1 exon 74425577 74426150 74425577 ID=Msa0374890-mRNA-1:exon:17212;Parent=Msa0374890-mRNA-1
chr3_1 exon 74426760 74427139 74426760 ID=Msa0374890-mRNA-1:exon:17213;Parent=Msa0374890-mRNA-1
chr3_1 CDS 74425577 74426150 74425577 ID=Msa0374890-mRNA-1:cds;Parent=Msa0374890-mRNA-1
chr3_1 CDS 74426760 74426818 74426760 ID=Msa0374890-mRNA-1:cds;Parent=Msa0374890-mRNA-1
chr3_1 three_prime_UTR 74426819 74427139 74426819 ID=Msa0374890-mRNA-1:three_prime_utr;Parent=Msa0374890-mRNA-1
Gene Sequence

>Msa0374890

ATGCTTGATCCAACAAGAAAACACCACCAACCTAAGAATTCTTTTGATTTGTACGATGAAACCAGACATGAAACATGGGAAGCCACAAATTCTAATAATCTCTGGCCTTCAACACAGCCTCAATTGGACATGTATCACGATGAACAACCTAGATGGAGTGTGAACTCACCACATTACAGAAGCCTTTCCCCTGTTTCAAGAACAGAGGCCATAGTGAGAGGACAAAAAGAACTCATGGAAATGGTTCGGAACATGCCCGAATCAAACTATGAACTTTCATTGAAGGATCTTGTGGAACACCACCACAGGACCAGAGAAGAAAATACAGAGGAGCAGAAGGAGGAGGAGGAGAAGGAGAAGGAGAAGAAGAATTCTAGTGTCTATAATGGTGGCAAGAAGATTGATAATAAGAAGGTACATGTGAAGAAAAATGATGGGAAAATTGATCGTGATCGAGGTTTCTACCTGAAAGTGGGGTTACCTTTTTTTTCTTTAGGATCTAAAGAGAAAAAGAAGAAGAAAAAGGAGTCAAAAGTTTCACCAAGACCATCAATTTCTGATGGACCAGTAAAAGGCGTGAGAAGGGTGGAGGTATATGCTGGCCTTTGTTTCGCAAACCCAAAATCCAAATAA
Protein sequence

>Msa0374890

MLDPTRKHHQPKNSFDLYDETRHETWEATNSNNLWPSTQPQLDMYHDEQPRWSVNSPHYRSLSPVSRTEAIVRGQKELMEMVRNMPESNYELSLKDLVEHHHRTREENTEEQKEEEEKEKEKKNSSVYNGGKKIDNKKVHVKKNDGKIDRDRGFYLKVGLPFFSLGSKEKKKKKKESKVSPRPSISDGPVKGVRRVEVYAGLCFANPKSK*