AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0468140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0468140 MtrunA17_Chr3g0122371 97.561 123 3 0 1 123 1 123 1.48e-88 252
Msa0468140 MtrunA17_Chr8g0377491 90.244 123 12 0 1 123 1 123 1.13e-81 235
Msa0468140 MtrunA17_Chr2g0308331 80.851 94 12 1 1 94 1 88 2.46e-49 153
Msa0468140 MtrunA17_Chr7g0235151 35.246 122 71 5 3 119 12 130 3.73e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0468140 AT1G27970.1 82.114 123 22 0 1 123 4 126 5.30e-74 216
Msa0468140 AT1G27310.1 82.927 123 20 1 1 123 1 122 2.57e-73 214
Msa0468140 AT1G27970.2 81.356 118 22 0 1 118 4 121 1.22e-69 205
Msa0468140 AT1G11570.2 59.504 121 46 1 6 123 12 132 9.42e-47 147
Msa0468140 AT1G11570.3 59.504 121 46 1 6 123 33 153 1.24e-46 147
Msa0468140 AT1G11570.1 57.426 101 40 1 6 103 12 112 1.57e-34 116
Msa0468140 AT1G11570.4 57.426 101 40 1 6 103 33 133 2.59e-34 116
Msa0468140 AT5G48650.1 37.302 126 70 6 1 120 11 133 2.73e-12 62.4

Find 68 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTTGCATCTAAAATT+TGG 0.111315 3_3:-69632156 Msa0468140:three_prime_UTR
CAGACATTTAAAATTAAAAT+TGG 0.120296 3_3:-69632307 Msa0468140:three_prime_UTR
AGATGCAAATTGCATGAATA+TGG 0.182084 3_3:+69632165 None:intergenic
CTTTGAAGGTCAGCAAATTA+TGG 0.205195 3_3:-69634131 Msa0468140:CDS
AAAATTAAAATTGGGTCTTT+TGG 0.234324 3_3:-69632298 Msa0468140:three_prime_UTR
TGTTGAAGATCAATGAATAT+TGG 0.239280 3_3:-69632211 Msa0468140:three_prime_UTR
GTTGAAGATCAATGAATATT+GGG 0.271194 3_3:-69632210 Msa0468140:three_prime_UTR
ATTGCTTGGATATATATAAT+TGG 0.283570 3_3:-69632027 Msa0468140:three_prime_UTR
TTTAATTTCTGCTTAGTGTT+TGG 0.317853 3_3:-69631829 None:intergenic
CAGCGACAATTCAAATTTAG+AGG 0.334225 3_3:-69631959 Msa0468140:three_prime_UTR
ATACAAGACTTGCTTTAGAT+TGG 0.359930 3_3:+69631900 None:intergenic
TTTGAAGGTCAGCAAATTAT+GGG 0.362536 3_3:-69634130 Msa0468140:CDS
AGCCATTCCAATTGATTGCT+TGG 0.382527 3_3:-69632041 Msa0468140:three_prime_UTR
AATGCCTTTGACAATGCGTT+TGG 0.385167 3_3:+69634232 None:intergenic
ATTGTGTTTAAACTCTTTGA+AGG 0.385926 3_3:-69632240 Msa0468140:three_prime_UTR
CTCTATCTCACTCCTTGGCC+TGG 0.398404 3_3:+69634316 None:intergenic
GGTTCTATGCTCACCTTTGA+AGG 0.402799 3_3:-69634145 Msa0468140:CDS
CGTGTTGTTCGCCGGCGAGC+TGG 0.403242 3_3:+69633981 None:intergenic
TATATATCCAAGCAATCAAT+TGG 0.405869 3_3:+69632034 None:intergenic
CGCATTGTCAAAGGCATTCG+TGG 0.412022 3_3:-69634227 Msa0468140:CDS
CATAATTTGCTGACCTTCAA+AGG 0.412917 3_3:+69634132 None:intergenic
GTTTAAACACAATTCTAAAC+AGG 0.413628 3_3:+69632250 None:intergenic
AGACATTTAAAATTAAAATT+GGG 0.414797 3_3:-69632306 Msa0468140:three_prime_UTR
CTTGCGGTGTTGGTATCAAA+TGG 0.429115 3_3:+69632425 None:intergenic
TTGGTAGAGAGTGGCGAGAT+TGG 0.433392 3_3:+69634171 None:intergenic
TGGTAGAGAGTGGCGAGATT+GGG 0.451654 3_3:+69634172 None:intergenic
TTCTTCACGCATAGTTCAAA+CGG 0.464644 3_3:+69632374 None:intergenic
TGATGGAGTGATGGCACTGC+TGG 0.466864 3_3:+69634068 None:intergenic
AATTGACCTGACTGAATTTG+AGG 0.471209 3_3:+69633957 None:intergenic
ATTTGATTTCATTGCTGAGT+TGG 0.473341 3_3:-69632096 Msa0468140:three_prime_UTR
TTTGAGGGCGTGTTGTTCGC+CGG 0.484969 3_3:+69633973 None:intergenic
TTGAAGATCAATGAATATTG+GGG 0.510000 3_3:-69632209 Msa0468140:three_prime_UTR
GCGCTTATTTATAAGAAGCC+AGG 0.512620 3_3:-69634334 Msa0468140:five_prime_UTR
ACCGTCGATTGTCAACCTTC+TGG 0.514517 3_3:-69634043 Msa0468140:CDS
ATTGGGACGGTTGGTGTCGA+AGG 0.521383 3_3:+69634189 None:intergenic
GAGTGATGGCACTGCTGGAA+AGG 0.524413 3_3:+69634073 None:intergenic
ATTATTGCAGTGTCATGCTT+AGG 0.526314 3_3:-69631860 Msa0468140:three_prime_UTR
TTGACAATCGACGGTGGTGA+TGG 0.528680 3_3:+69634051 None:intergenic
TAATAGCTTCCTTGCGGTGT+TGG 0.531758 3_3:+69632415 None:intergenic
ATTGACCTGACTGAATTTGA+GGG 0.536270 3_3:+69633958 None:intergenic
AGTCTCTCTATCTCACTCCT+TGG 0.546043 3_3:+69634311 None:intergenic
ATTTATAAGAAGCCAGGCCA+AGG 0.547142 3_3:-69634328 Msa0468140:five_prime_UTR
ATGCCGCCGTTAGCGCCAGA+AGG 0.549119 3_3:+69634028 None:intergenic
ATGGCTAGCACACTTTGTGC+TGG 0.551762 3_3:+69632058 None:intergenic
TGTCAACCTTCTGGCGCTAA+CGG 0.557071 3_3:-69634034 Msa0468140:CDS
AGTGGCGAGATTGGGACGGT+TGG 0.563337 3_3:+69634180 None:intergenic
ATCCAAGCAATCAATTGGAA+TGG 0.565549 3_3:+69632039 None:intergenic
GGGACGGTTGGTGTCGAAGG+TGG 0.565989 3_3:+69634192 None:intergenic
AGGTGAGCATAGAACCATCT+TGG 0.568405 3_3:+69634152 None:intergenic
CTCGCCACTCTCTACCAAGA+TGG 0.569246 3_3:-69634166 Msa0468140:CDS
ACACGCCCTCAAATTCAGTC+AGG 0.570302 3_3:-69633963 Msa0468140:intron
CAGAGAAAGAGATCGTGTTA+CGG 0.571732 3_3:-69634274 Msa0468140:five_prime_UTR
GGATCCAAACGCATTGTCAA+AGG 0.574827 3_3:-69634236 Msa0468140:CDS
ACTTTGTGCTGGCTACATAA+CGG 0.577022 3_3:+69632069 None:intergenic
TTACGGCGGCGTAAGAGCGA+TGG 0.580005 3_3:-69634257 Msa0468140:exon
AGTGGTAATCTCCAGCTCGC+CGG 0.581878 3_3:-69633992 Msa0468140:CDS
AGTGATGGCACTGCTGGAAA+GGG 0.591260 3_3:+69634074 None:intergenic
GGCATGCTTGTCTTTGTCAG+TGG 0.596448 3_3:-69634010 Msa0468140:CDS
CATTTGATACCAACACCGCA+AGG 0.621557 3_3:-69632424 Msa0468140:CDS
GCCAGAAGGTTGACAATCGA+CGG 0.623192 3_3:+69634042 None:intergenic
AGAACCATCTTGGTAGAGAG+TGG 0.624728 3_3:+69634162 None:intergenic
CGACGGTGGTGATGGAGTGA+TGG 0.635677 3_3:+69634059 None:intergenic
AACACATAATAGCTTCCTTG+CGG 0.638641 3_3:+69632409 None:intergenic
CAACCTTCTGGCGCTAACGG+CGG 0.643186 3_3:-69634031 Msa0468140:CDS
AGAGAGTGGCGAGATTGGGA+CGG 0.652017 3_3:+69634176 None:intergenic
AGAAAGAGATCGTGTTACGG+CGG 0.704735 3_3:-69634271 Msa0468140:five_prime_UTR
TGAAGATCAATGAATATTGG+GGG 0.706123 3_3:-69632208 Msa0468140:three_prime_UTR
AGAAGGTTGACAATCGACGG+TGG 0.721439 3_3:+69634045 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTATTTGTTAATTTTA+GGG - chr3_3:69632334-69632353 Msa0468140:three_prime_UTR 10.0%
!!! AGACATTTAAAATTAAAATT+GGG - chr3_3:69633944-69633963 Msa0468140:intron 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr3_3:69633464-69633483 Msa0468140:intron 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr3_3:69633464-69633483 Msa0468140:intron 100.0%
! GGGGGGGGGGGGGGGGGGGG+AGG - chr3_3:69633464-69633483 Msa0468140:intron 100.0%
!!! GTTTTCTATTTGTTAATTTT+AGG - chr3_3:69632333-69632352 Msa0468140:three_prime_UTR 15.0%
!!! TTTCATGATTAAGTTTTAAT+TGG - chr3_3:69632405-69632424 Msa0468140:CDS 15.0%
!!! GTATTATCAATATTCTAAAA+TGG - chr3_3:69632626-69632645 Msa0468140:intron 15.0%
!!! ATTAACATTTTACAATTCTA+AGG + chr3_3:69632653-69632672 None:intergenic 15.0%
!! AGAATTGTAAAATGTTAATT+AGG - chr3_3:69632654-69632673 Msa0468140:intron 15.0%
!!! TTTTATTTTAGTGATCATTT+TGG - chr3_3:69632907-69632926 Msa0468140:intron 15.0%
!! TTGATATAATCAACAATTTA+AGG - chr3_3:69632956-69632975 Msa0468140:intron 15.0%
!! TGATATAATCAACAATTTAA+GGG - chr3_3:69632957-69632976 Msa0468140:intron 15.0%
!! AAATCCATCATAATAATAAT+AGG + chr3_3:69633000-69633019 None:intergenic 15.0%
!!! TATTATTATTATGATGGATT+TGG - chr3_3:69632999-69633018 Msa0468140:intron 15.0%
!!! CAGACATTTAAAATTAAAAT+TGG - chr3_3:69633943-69633962 Msa0468140:intron 15.0%
!! CATGAAAAACTTAAATAACA+CGG + chr3_3:69632392-69632411 None:intergenic 20.0%
!! ATAATACCAATCAAATTTCA+GGG + chr3_3:69632613-69632632 None:intergenic 20.0%
!!! TGGAATATTGTTATTGTAAA+TGG - chr3_3:69632927-69632946 Msa0468140:intron 20.0%
!! TTTGCCTATTATTATTATGA+TGG - chr3_3:69632993-69633012 Msa0468140:intron 20.0%
!!! TAGAGTGAAATTTCTAATTT+TGG - chr3_3:69633561-69633580 Msa0468140:intron 20.0%
!!! AAAATTAAAATTGGGTCTTT+TGG - chr3_3:69633952-69633971 Msa0468140:intron 20.0%
!! ATTGCTTGGATATATATAAT+TGG - chr3_3:69634223-69634242 Msa0468140:CDS 20.0%
!!! CAATAGTATTTTAAAAGAGA+AGG + chr3_3:69634260-69634279 None:intergenic 20.0%
!!! CATGATTAAGTTTTAATTGG+TGG - chr3_3:69632408-69632427 Msa0468140:CDS 25.0%
! GATAATACCAATCAAATTTC+AGG + chr3_3:69632614-69632633 None:intergenic 25.0%
!! AATTAGGTTTTTCTATCTGT+TGG - chr3_3:69632670-69632689 Msa0468140:intron 25.0%
! TTGGAATGTCAAAATTACAT+AGG + chr3_3:69632780-69632799 None:intergenic 25.0%
! AACAATTTAAGGGACTAAAT+TGG - chr3_3:69632967-69632986 Msa0468140:intron 25.0%
! AAAACAACATTGATACAAAG+AGG + chr3_3:69633092-69633111 None:intergenic 25.0%
! TGATGTTGTTGAACATTTAT+TGG - chr3_3:69633136-69633155 Msa0468140:intron 25.0%
! GATGTTGTTGAACATTTATT+GGG - chr3_3:69633137-69633156 Msa0468140:intron 25.0%
! TGAACAAATCAAAGCATAAA+AGG + chr3_3:69633244-69633263 None:intergenic 25.0%
! CATATTAACTAGCATTAGAT+TGG + chr3_3:69633267-69633286 None:intergenic 25.0%
!!! AAATATTGGTTAACTTGTCA+GGG - chr3_3:69633419-69633438 Msa0468140:intron 25.0%
! ATTTCACTCTAATCATGAAT+TGG + chr3_3:69633552-69633571 None:intergenic 25.0%
!! AGTTTTCCTCATATTGATAA+TGG - chr3_3:69633584-69633603 Msa0468140:intron 25.0%
! AAAACACCATTATCAATATG+AGG + chr3_3:69633593-69633612 None:intergenic 25.0%
! CTTCATATGTTACTGAAATT+TGG + chr3_3:69633635-69633654 None:intergenic 25.0%
! CAAATTTCAGTAACATATGA+AGG - chr3_3:69633633-69633652 Msa0468140:intron 25.0%
! TCTATCTGGACAATAATAAA+TGG + chr3_3:69633678-69633697 None:intergenic 25.0%
! GTTAAATCATCAATATCACT+TGG - chr3_3:69633707-69633726 Msa0468140:intron 25.0%
! GTTTAAACACAATTCTAAAC+AGG + chr3_3:69634003-69634022 None:intergenic 25.0%
! ATTGTGTTTAAACTCTTTGA+AGG - chr3_3:69634010-69634029 Msa0468140:CDS 25.0%
! TGTTGAAGATCAATGAATAT+TGG - chr3_3:69634039-69634058 Msa0468140:CDS 25.0%
! GTTGAAGATCAATGAATATT+GGG - chr3_3:69634040-69634059 Msa0468140:CDS 25.0%
! TTGAAGATCAATGAATATTG+GGG - chr3_3:69634041-69634060 Msa0468140:CDS 25.0%
!! TGCAATTTGCATCTAAAATT+TGG - chr3_3:69634094-69634113 Msa0468140:CDS 25.0%
! TATATATCCAAGCAATCAAT+TGG + chr3_3:69634219-69634238 None:intergenic 25.0%
TTTGAAGGTCAGCAAATTAT+GGG - chr3_3:69632120-69632139 Msa0468140:three_prime_UTR 30.0%
! TTGCAACTTTATCTGGTTTA+GGG - chr3_3:69632490-69632509 Msa0468140:intron 30.0%
TCAAATTTCAGGGATCAAAT+TGG + chr3_3:69632603-69632622 None:intergenic 30.0%
TTTGATCCCTGAAATTTGAT+TGG - chr3_3:69632604-69632623 Msa0468140:intron 30.0%
TGTCATGTTAGAAATGCATT+AGG + chr3_3:69632817-69632836 None:intergenic 30.0%
TTCTAACATGACAAACGTAT+CGG - chr3_3:69632824-69632843 Msa0468140:intron 30.0%
AGTTAGATTCATCTCTTTGT+TGG + chr3_3:69632870-69632889 None:intergenic 30.0%
!!! AATGTTGTTTTCTCTAGTCT+AGG - chr3_3:69633101-69633120 Msa0468140:intron 30.0%
!!! ATGTTGTTTTCTCTAGTCTA+GGG - chr3_3:69633102-69633121 Msa0468140:intron 30.0%
! GAAATAACTAAGAAAGCACA+TGG + chr3_3:69633320-69633339 None:intergenic 30.0%
! AGTTATTTCACTCAGATTTC+AGG - chr3_3:69633331-69633350 Msa0468140:intron 30.0%
TATTTCGTATAGAAGTAGGA+TGG + chr3_3:69633358-69633377 None:intergenic 30.0%
TCACTATTTCGTATAGAAGT+AGG + chr3_3:69633362-69633381 None:intergenic 30.0%
!! CAAATATTGGTTAACTTGTC+AGG - chr3_3:69633418-69633437 Msa0468140:intron 30.0%
! TGAAATTTGGTCTTTCTCTA+TGG + chr3_3:69633622-69633641 None:intergenic 30.0%
ATTTCAGTAACATATGAAGG+CGG - chr3_3:69633636-69633655 Msa0468140:intron 30.0%
TTAACAATCACAGTTCTATC+TGG + chr3_3:69633692-69633711 None:intergenic 30.0%
CAAAATTGAGAAATAAGAGC+AGG + chr3_3:69633767-69633786 None:intergenic 30.0%
TGAAGATCAATGAATATTGG+GGG - chr3_3:69634042-69634061 Msa0468140:CDS 30.0%
AGATGCAAATTGCATGAATA+TGG + chr3_3:69634088-69634107 None:intergenic 30.0%
! ATTTGATTTCATTGCTGAGT+TGG - chr3_3:69634154-69634173 Msa0468140:CDS 30.0%
! ATACAAGACTTGCTTTAGAT+TGG + chr3_3:69634353-69634372 None:intergenic 30.0%
!! CATAATTTGCTGACCTTCAA+AGG + chr3_3:69632121-69632140 None:intergenic 35.0%
! CTTTGAAGGTCAGCAAATTA+TGG - chr3_3:69632119-69632138 Msa0468140:three_prime_UTR 35.0%
ATTGACCTGACTGAATTTGA+GGG + chr3_3:69632295-69632314 None:intergenic 35.0%
AATTGACCTGACTGAATTTG+AGG + chr3_3:69632296-69632315 None:intergenic 35.0%
! GTTGCAACTTTATCTGGTTT+AGG - chr3_3:69632489-69632508 Msa0468140:intron 35.0%
!! GTTAATAGTTTTGCTGTCGT+AGG - chr3_3:69632555-69632574 Msa0468140:intron 35.0%
CTGTCGTAGGATTTCAATAT+TGG - chr3_3:69632568-69632587 Msa0468140:intron 35.0%
TCATTGTTCATTTGCATGCT+TGG - chr3_3:69632722-69632741 Msa0468140:intron 35.0%
TTAGGAGCTAAGTGTGTATT+TGG + chr3_3:69632799-69632818 None:intergenic 35.0%
GATACAAAGAGGAATCTACA+TGG + chr3_3:69633081-69633100 None:intergenic 35.0%
GTTGTTGAACATTTATTGGG+TGG - chr3_3:69633140-69633159 Msa0468140:intron 35.0%
GAGAGCAATTTAACAAAACC+AGG + chr3_3:69633187-69633206 None:intergenic 35.0%
TCACTTCCTTTGCGATAAAA+GGG + chr3_3:69633394-69633413 None:intergenic 35.0%
TTCACTTCCTTTGCGATAAA+AGG + chr3_3:69633395-69633414 None:intergenic 35.0%
TTTATCGCAAAGGAAGTGAA+TGG - chr3_3:69633395-69633414 Msa0468140:intron 35.0%
AGGAAGTGAATGGCAAATAT+TGG - chr3_3:69633405-69633424 Msa0468140:intron 35.0%
ATCACTAAATTCTCGTTCTG+TGG - chr3_3:69633497-69633516 Msa0468140:intron 35.0%
TAAATTCTCGTTCTGTGGTT+GGG - chr3_3:69633502-69633521 Msa0468140:intron 35.0%
TCACTCTAATCATGAATTGG+TGG + chr3_3:69633549-69633568 None:intergenic 35.0%
TGAGAAATAAGAGCAGGATT+AGG + chr3_3:69633761-69633780 None:intergenic 35.0%
AACACATAATAGCTTCCTTG+CGG + chr3_3:69633844-69633863 None:intergenic 35.0%
TTCTTCACGCATAGTTCAAA+CGG + chr3_3:69633879-69633898 None:intergenic 35.0%
ATCCAAGCAATCAATTGGAA+TGG + chr3_3:69634214-69634233 None:intergenic 35.0%
CAGCGACAATTCAAATTTAG+AGG - chr3_3:69634291-69634310 Msa0468140:five_prime_UTR 35.0%
ATTATTGCAGTGTCATGCTT+AGG - chr3_3:69634390-69634409 Msa0468140:five_prime_UTR 35.0%
GCGCTTATTTATAAGAAGCC+AGG - chr3_3:69631916-69631935 Msa0468140:three_prime_UTR 40.0%
ATTTATAAGAAGCCAGGCCA+AGG - chr3_3:69631922-69631941 Msa0468140:three_prime_UTR 40.0%
CAGAGAAAGAGATCGTGTTA+CGG - chr3_3:69631976-69631995 Msa0468140:three_prime_UTR 40.0%
AATGCCTTTGACAATGCGTT+TGG + chr3_3:69632021-69632040 None:intergenic 40.0%
TCTAGCGTTGCAACTTTATC+TGG - chr3_3:69632483-69632502 Msa0468140:intron 40.0%
CATTTGCATGCTTGGATTTG+CGG - chr3_3:69632730-69632749 Msa0468140:intron 40.0%
TGAACATTTATTGGGTGGAG+TGG - chr3_3:69633145-69633164 Msa0468140:intron 40.0%
GAACATTTATTGGGTGGAGT+GGG - chr3_3:69633146-69633165 Msa0468140:intron 40.0%
AACATTTATTGGGTGGAGTG+GGG - chr3_3:69633147-69633166 Msa0468140:intron 40.0%
TTCCTTTGCGATAAAAGGGA+TGG + chr3_3:69633390-69633409 None:intergenic 40.0%
CTAAATTCTCGTTCTGTGGT+TGG - chr3_3:69633501-69633520 Msa0468140:intron 40.0%
GGGGGTTGAAAACTTTATAC+TGG - chr3_3:69634060-69634079 Msa0468140:CDS 40.0%
!! ACTTTGTGCTGGCTACATAA+CGG + chr3_3:69634184-69634203 None:intergenic 40.0%
AGCCATTCCAATTGATTGCT+TGG - chr3_3:69634209-69634228 Msa0468140:CDS 40.0%
AGTCTCTCTATCTCACTCCT+TGG + chr3_3:69631942-69631961 None:intergenic 45.0%
AGAAAGAGATCGTGTTACGG+CGG - chr3_3:69631979-69631998 Msa0468140:three_prime_UTR 45.0%
GGATCCAAACGCATTGTCAA+AGG - chr3_3:69632014-69632033 Msa0468140:three_prime_UTR 45.0%
AGAACCATCTTGGTAGAGAG+TGG + chr3_3:69632091-69632110 None:intergenic 45.0%
AGGTGAGCATAGAACCATCT+TGG + chr3_3:69632101-69632120 None:intergenic 45.0%
GGTTCTATGCTCACCTTTGA+AGG - chr3_3:69632105-69632124 Msa0468140:three_prime_UTR 45.0%
TCAATTCCCTCTCTCTCTCT+AGG - chr3_3:69632310-69632329 Msa0468140:three_prime_UTR 45.0%
CAATTCCCTCTCTCTCTCTA+GGG - chr3_3:69632311-69632330 Msa0468140:three_prime_UTR 45.0%
GAAAACCCTAGAGAGAGAGA+GGG + chr3_3:69632319-69632338 None:intergenic 45.0%
AGAAAACCCTAGAGAGAGAG+AGG + chr3_3:69632320-69632339 None:intergenic 45.0%
ACATTTATTGGGTGGAGTGG+GGG - chr3_3:69633148-69633167 Msa0468140:intron 45.0%
AGGCACGTTCTTTCTAGACA+TGG + chr3_3:69633224-69633243 None:intergenic 45.0%
GCTAGTTAATATGCGCCTTG+TGG - chr3_3:69633274-69633293 Msa0468140:intron 45.0%
AATATGCGCCTTGTGGTCAA+TGG - chr3_3:69633281-69633300 Msa0468140:intron 45.0%
TCCAAGAACCATTGACCACA+AGG + chr3_3:69633292-69633311 None:intergenic 45.0%
! GACCATCCCTTTTATCGCAA+AGG - chr3_3:69633385-69633404 Msa0468140:intron 45.0%
TGGTTGGGCAATGGGAATTA+GGG - chr3_3:69633517-69633536 Msa0468140:intron 45.0%
!! CTTGCGGTGTTGGTATCAAA+TGG + chr3_3:69633828-69633847 None:intergenic 45.0%
CATTTGATACCAACACCGCA+AGG - chr3_3:69633826-69633845 Msa0468140:intron 45.0%
TAATAGCTTCCTTGCGGTGT+TGG + chr3_3:69633838-69633857 None:intergenic 45.0%
!! CGCATTGTCAAAGGCATTCG+TGG - chr3_3:69632023-69632042 Msa0468140:three_prime_UTR 50.0%
TGGTAGAGAGTGGCGAGATT+GGG + chr3_3:69632081-69632100 None:intergenic 50.0%
TTGGTAGAGAGTGGCGAGAT+TGG + chr3_3:69632082-69632101 None:intergenic 50.0%
AGTGATGGCACTGCTGGAAA+GGG + chr3_3:69632179-69632198 None:intergenic 50.0%
TTGACAATCGACGGTGGTGA+TGG + chr3_3:69632202-69632221 None:intergenic 50.0%
AGAAGGTTGACAATCGACGG+TGG + chr3_3:69632208-69632227 None:intergenic 50.0%
! ACCGTCGATTGTCAACCTTC+TGG - chr3_3:69632207-69632226 Msa0468140:three_prime_UTR 50.0%
GCCAGAAGGTTGACAATCGA+CGG + chr3_3:69632211-69632230 None:intergenic 50.0%
TGTCAACCTTCTGGCGCTAA+CGG - chr3_3:69632216-69632235 Msa0468140:three_prime_UTR 50.0%
GGCATGCTTGTCTTTGTCAG+TGG - chr3_3:69632240-69632259 Msa0468140:three_prime_UTR 50.0%
ACACGCCCTCAAATTCAGTC+AGG - chr3_3:69632287-69632306 Msa0468140:three_prime_UTR 50.0%
GCTTGGATTTGCGGTGAGTT+TGG - chr3_3:69632739-69632758 Msa0468140:intron 50.0%
GCCTTGTGGTCAATGGTTCT+TGG - chr3_3:69633288-69633307 Msa0468140:intron 50.0%
ACCCATCTTGAAGGGTCTGT+TGG - chr3_3:69633443-69633462 Msa0468140:intron 50.0%
CCCAACAGACCCTTCAAGAT+GGG + chr3_3:69633447-69633466 None:intergenic 50.0%
CCCATCTTGAAGGGTCTGTT+GGG - chr3_3:69633444-69633463 Msa0468140:intron 50.0%
CCATCTTGAAGGGTCTGTTG+GGG - chr3_3:69633445-69633464 Msa0468140:intron 50.0%
CATCTTGAAGGGTCTGTTGG+GGG - chr3_3:69633446-69633465 Msa0468140:intron 50.0%
ATCTTGAAGGGTCTGTTGGG+GGG - chr3_3:69633447-69633466 Msa0468140:intron 50.0%
TCGTTCTGTGGTTGGGCAAT+GGG - chr3_3:69633509-69633528 Msa0468140:intron 50.0%
GTGGTTGGGCAATGGGAATT+AGG - chr3_3:69633516-69633535 Msa0468140:intron 50.0%
ATGGCTAGCACACTTTGTGC+TGG + chr3_3:69634195-69634214 None:intergenic 50.0%
CTCTATCTCACTCCTTGGCC+TGG + chr3_3:69631937-69631956 None:intergenic 55.0%
!! ATTGGGACGGTTGGTGTCGA+AGG + chr3_3:69632064-69632083 None:intergenic 55.0%
AGAGAGTGGCGAGATTGGGA+CGG + chr3_3:69632077-69632096 None:intergenic 55.0%
CTCGCCACTCTCTACCAAGA+TGG - chr3_3:69632084-69632103 Msa0468140:three_prime_UTR 55.0%
GAGTGATGGCACTGCTGGAA+AGG + chr3_3:69632180-69632199 None:intergenic 55.0%
TGATGGAGTGATGGCACTGC+TGG + chr3_3:69632185-69632204 None:intergenic 55.0%
AGTGGTAATCTCCAGCTCGC+CGG - chr3_3:69632258-69632277 Msa0468140:three_prime_UTR 55.0%
! TTTGAGGGCGTGTTGTTCGC+CGG + chr3_3:69632280-69632299 None:intergenic 55.0%
!! TGGAGTGGGGGAAGTGTTTG+TGG - chr3_3:69633160-69633179 Msa0468140:intron 55.0%
! TGTCAGGGCACCCATCTTGA+AGG - chr3_3:69633434-69633453 Msa0468140:intron 55.0%
! GTCAGGGCACCCATCTTGAA+GGG - chr3_3:69633435-69633454 Msa0468140:intron 55.0%
CCCCAACAGACCCTTCAAGA+TGG + chr3_3:69633448-69633467 None:intergenic 55.0%
TCTTGAAGGGTCTGTTGGGG+GGG - chr3_3:69633448-69633467 Msa0468140:intron 55.0%
CTCGTTCTGTGGTTGGGCAA+TGG - chr3_3:69633508-69633527 Msa0468140:intron 55.0%
TTACGGCGGCGTAAGAGCGA+TGG - chr3_3:69631993-69632012 Msa0468140:three_prime_UTR 60.0%
AGTGGCGAGATTGGGACGGT+TGG + chr3_3:69632073-69632092 None:intergenic 60.0%
!! CGACGGTGGTGATGGAGTGA+TGG + chr3_3:69632194-69632213 None:intergenic 60.0%
CAACCTTCTGGCGCTAACGG+CGG - chr3_3:69632219-69632238 Msa0468140:three_prime_UTR 60.0%
!! GGGGGAAGTGTTTGTGGTCC+TGG - chr3_3:69633166-69633185 Msa0468140:intron 60.0%
CTTGAAGGGTCTGTTGGGGG+GGG - chr3_3:69633449-69633468 Msa0468140:intron 60.0%
TTGAAGGGTCTGTTGGGGGG+GGG - chr3_3:69633450-69633469 Msa0468140:intron 60.0%
!! GGGACGGTTGGTGTCGAAGG+TGG + chr3_3:69632061-69632080 None:intergenic 65.0%
ATGCCGCCGTTAGCGCCAGA+AGG + chr3_3:69632225-69632244 None:intergenic 65.0%
TGAAGGGTCTGTTGGGGGGG+GGG - chr3_3:69633451-69633470 Msa0468140:intron 65.0%
! CGTGTTGTTCGCCGGCGAGC+TGG + chr3_3:69632272-69632291 None:intergenic 70.0%
GAAGGGTCTGTTGGGGGGGG+GGG - chr3_3:69633452-69633471 Msa0468140:intron 70.0%
AAGGGTCTGTTGGGGGGGGG+GGG - chr3_3:69633453-69633472 Msa0468140:intron 70.0%
AGGGTCTGTTGGGGGGGGGG+GGG - chr3_3:69633454-69633473 Msa0468140:intron 75.0%
! GGGTCTGTTGGGGGGGGGGG+GGG - chr3_3:69633455-69633474 Msa0468140:intron 80.0%
! GGTCTGTTGGGGGGGGGGGG+GGG - chr3_3:69633456-69633475 Msa0468140:intron 80.0%
! GTCTGTTGGGGGGGGGGGGG+GGG - chr3_3:69633457-69633476 Msa0468140:intron 80.0%
! TCTGTTGGGGGGGGGGGGGG+GGG - chr3_3:69633458-69633477 Msa0468140:intron 80.0%
! CTGTTGGGGGGGGGGGGGGG+GGG - chr3_3:69633459-69633478 Msa0468140:intron 85.0%
! TGTTGGGGGGGGGGGGGGGG+GGG - chr3_3:69633460-69633479 Msa0468140:intron 85.0%
! GTTGGGGGGGGGGGGGGGGG+GGG - chr3_3:69633461-69633480 Msa0468140:intron 90.0%
! TTGGGGGGGGGGGGGGGGGG+GGG - chr3_3:69633462-69633481 Msa0468140:intron 90.0%
! TGGGGGGGGGGGGGGGGGGG+GGG - chr3_3:69633463-69633482 Msa0468140:intron 95.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 69631830 69634442 69631830 ID=Msa0468140;Name=Msa0468140
chr3_3 mRNA 69631830 69634442 69631830 ID=Msa0468140-mRNA-1;Parent=Msa0468140;Name=Msa0468140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=182|1|1|1|1|1|2|548|123
chr3_3 exon 69631830 69632452 69631830 ID=Msa0468140-mRNA-1:exon:16534;Parent=Msa0468140-mRNA-1
chr3_3 exon 69633964 69634442 69633964 ID=Msa0468140-mRNA-1:exon:16533;Parent=Msa0468140-mRNA-1
chr3_3 five_prime_UTR 69634261 69634442 69634261 ID=Msa0468140-mRNA-1:five_prime_utr;Parent=Msa0468140-mRNA-1
chr3_3 CDS 69633964 69634260 69633964 ID=Msa0468140-mRNA-1:cds;Parent=Msa0468140-mRNA-1
chr3_3 CDS 69632378 69632452 69632378 ID=Msa0468140-mRNA-1:cds;Parent=Msa0468140-mRNA-1
chr3_3 three_prime_UTR 69631830 69632377 69631830 ID=Msa0468140-mRNA-1:three_prime_utr;Parent=Msa0468140-mRNA-1
Gene Sequence

>Msa0468140

ATGGATCCAAACGCATTGTCAAAGGCATTCGTGGAACACTACTACACCACCTTCGACACCAACCGTCCCAATCTCGCCACTCTCTACCAAGATGGTTCTATGCTCACCTTTGAAGGTCAGCAAATTATGGGATCTCAGAATATCGTCGCTAAACTCACTTCTCTCCCTTTCCAGCAGTGCCATCACTCCATCACCACCGTCGATTGTCAACCTTCTGGCGCTAACGGCGGCATGCTTGTCTTTGTCAGTGGTAATCTCCAGCTCGCCGGCGAACAACACGCCCTCAAATTCAGTCAGATGTTCCATTTGATACCAACACCGCAAGGAAGCTATTATGTGTTGAATGACATATTCCGTTTGAACTATGCGTGA
Protein sequence

>Msa0468140

MDPNALSKAFVEHYYTTFDTNRPNLATLYQDGSMLTFEGQQIMGSQNIVAKLTSLPFQQCHHSITTVDCQPSGANGGMLVFVSGNLQLAGEQHALKFSQMFHLIPTPQGSYYVLNDIFRLNYA*