AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0881240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0881240 MtrunA17_Chr4g0063171 100.000 78 0 0 1 78 1 78 8.64e-52 156
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0881240 AT1G68680.1 70.667 75 20 1 4 78 3 75 5.27e-31 103

Find 42 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAAAATTTCTCTATTTA+AGG 0.064089 6_1:-28271436 None:intergenic
AGTAGAATAATTATGGAATT+TGG 0.210855 6_1:+28271549 Msa0881240:three_prime_UTR
CGAGTAAAATATGGTCTAAT+TGG 0.226747 6_1:+28271612 Msa0881240:three_prime_UTR
ATTCTGGTCAATTAAGAAAA+AGG 0.227301 6_1:+28268168 None:intergenic
ACTTGTGAATAATTTATATA+TGG 0.326133 6_1:+28271724 Msa0881240:three_prime_UTR
GACTAACTTAGAGGGAGAAC+AGG 0.369848 6_1:-28268458 None:intergenic
TTTCAGTTCGTTTGGGGTTG+CGG 0.370415 6_1:+28268555 Msa0881240:intron
TACAATTCTCGAATAGTATA+TGG 0.376764 6_1:-28271502 None:intergenic
GAGCATATATGAGAGGAAAA+AGG 0.391598 6_1:-28268436 None:intergenic
GTTATAATGCGAGTAAAATA+TGG 0.396809 6_1:+28271603 Msa0881240:three_prime_UTR
TTGTTCTTCCTTCTTGCAAC+GGG 0.436059 6_1:-28268395 None:intergenic
GAGCAAATAAGATTTATATC+AGG 0.443330 6_1:-28271681 None:intergenic
AACAAACGATCTCTCAAAAC+AGG 0.448254 6_1:-28268584 None:intergenic
TTGTTTACAGCTGTCTTGGC+TGG 0.452873 6_1:+28268602 Msa0881240:CDS
TCGAGAGTTGTTGAAACAGC+AGG 0.457017 6_1:-28268238 None:intergenic
GATATAAATCTTATTTGCTC+TGG 0.460790 6_1:+28271684 Msa0881240:three_prime_UTR
TCGTTTGTTTACAGCTGTCT+TGG 0.473619 6_1:+28268598 Msa0881240:CDS
TCTCTATTTAAGGTGTTGCC+AGG 0.473641 6_1:-28271426 None:intergenic
TAATTTATATATGGTCTAAG+TGG 0.475890 6_1:+28271733 Msa0881240:three_prime_UTR
ATTGTTCTTCCTTCTTGCAA+CGG 0.484891 6_1:-28268396 None:intergenic
TGATTATACAGAGCATGTAT+TGG 0.486189 6_1:+28271327 Msa0881240:exon
GTGTTTGCTGCTCAAAATCC+TGG 0.487476 6_1:+28271408 Msa0881240:three_prime_UTR
CGCATTATAACACTTGGAGT+TGG 0.491659 6_1:-28271591 None:intergenic
ATCGTTTACATCACAACCTC+AGG 0.501379 6_1:+28271363 Msa0881240:three_prime_UTR
AGAAAACGTTACATGTTAGC+AGG 0.515067 6_1:-28268299 None:intergenic
AGAATAATTATGGAATTTGG+AGG 0.519417 6_1:+28271552 Msa0881240:three_prime_UTR
ACAAGTCAAATAATTTATTG+AGG 0.525568 6_1:-28271387 None:intergenic
ATCAGTGTGATTCATTGTAT+CGG 0.527719 6_1:+28268193 Msa0881240:five_prime_UTR
TTTACTCGCATTATAACACT+TGG 0.527900 6_1:-28271597 None:intergenic
ATTATAACACTTGGAGTTGG+AGG 0.531140 6_1:-28271588 None:intergenic
GTTTCAACAACTCTCGATCT+CGG 0.543448 6_1:+28268244 Msa0881240:five_prime_UTR
GGTTGTGATGTAAACGATGC+TGG 0.556905 6_1:-28271358 None:intergenic
AGTGGAGTGACTAACTTAGA+GGG 0.580435 6_1:-28268466 None:intergenic
TTTCAATTTCAGTTCGTTTG+GGG 0.590044 6_1:+28268549 Msa0881240:intron
AATAATTTATTGAGGACCTG+AGG 0.609349 6_1:-28271379 None:intergenic
ACAGAGAACCCGTTGCAAGA+AGG 0.610499 6_1:+28268387 Msa0881240:intron
GATCTCGGTCAATTCCGCCA+TGG 0.632156 6_1:+28268259 Msa0881240:exon
CGAGAGTTGTTGAAACAGCA+GGG 0.636152 6_1:-28268237 None:intergenic
AGAACAGGAGCATATATGAG+AGG 0.644560 6_1:-28268443 None:intergenic
GAACGACGTCGGCGCCATGG+CGG 0.678846 6_1:-28268273 None:intergenic
GTTGCAAGAAGGAAGAACAA+TGG 0.706424 6_1:+28268398 Msa0881240:CDS
TTAGCAGGATCGAACGACGT+CGG 0.711088 6_1:-28268284 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAATAAATAATATAA+TGG - chr6_1:28270829-28270848 None:intergenic 0.0%
!! ATTATATTATTTATTTAATT+AGG + chr6_1:28270828-28270847 Msa0881240:intron 0.0%
!! TAGAATAAATTAAAGAAAAA+AGG - chr6_1:28268659-28268678 None:intergenic 10.0%
!!! ACTGTATTTATCTTTTTTTT+AGG - chr6_1:28269507-28269526 None:intergenic 15.0%
!!! AGTCAAATTGTTTTTATATA+AGG + chr6_1:28269524-28269543 Msa0881240:intron 15.0%
!!! GTCATTTTTTTTTTTTTTTG+AGG - chr6_1:28270608-28270627 None:intergenic 15.0%
!! TGATAAAATTTCTCTATTTA+AGG - chr6_1:28271439-28271458 None:intergenic 15.0%
!!! ATTTTTTTTTCCCAATTTTT+TGG + chr6_1:28271466-28271485 Msa0881240:three_prime_UTR 15.0%
!!! TTTTTTTTTCCCAATTTTTT+GGG + chr6_1:28271467-28271486 Msa0881240:three_prime_UTR 15.0%
!! ACTTGTGAATAATTTATATA+TGG + chr6_1:28271724-28271743 Msa0881240:three_prime_UTR 15.0%
!!! TTTGAACATTTAGAATAGTT+TGG + chr6_1:28268861-28268880 Msa0881240:intron 20.0%
!!! TTGAACATTTAGAATAGTTT+GGG + chr6_1:28268862-28268881 Msa0881240:intron 20.0%
!! TGGTATATGATAGAAAATTA+TGG + chr6_1:28269282-28269301 Msa0881240:intron 20.0%
!!! ACTATTTTCATAAGTTATCT+TGG + chr6_1:28269552-28269571 Msa0881240:intron 20.0%
!! TTAATTGATATCAGAATCAA+TGG - chr6_1:28270653-28270672 None:intergenic 20.0%
!! TAGAATGAAATTACATTGAT+TGG + chr6_1:28271136-28271155 Msa0881240:intron 20.0%
!! ACAAGTCAAATAATTTATTG+AGG - chr6_1:28271390-28271409 None:intergenic 20.0%
!! AATAATCTTCCCAAAAAATT+GGG - chr6_1:28271479-28271498 None:intergenic 20.0%
!!! AGCTTTTAGTAGAATAATTA+TGG + chr6_1:28271542-28271561 Msa0881240:three_prime_UTR 20.0%
!! AGTAGAATAATTATGGAATT+TGG + chr6_1:28271549-28271568 Msa0881240:three_prime_UTR 20.0%
! ATTCATTGTATCGGAAAAAA+GGG + chr6_1:28268202-28268221 Msa0881240:five_prime_UTR 25.0%
! AGCAGTTAAAGAATTGATTA+AGG - chr6_1:28268362-28268381 None:intergenic 25.0%
! AATGAATTGAATGAAGAAAG+TGG - chr6_1:28268487-28268506 None:intergenic 25.0%
!! ATTTTCAATTTCAGTTCGTT+TGG + chr6_1:28268547-28268566 Msa0881240:intron 25.0%
!! TTTTCAATTTCAGTTCGTTT+GGG + chr6_1:28268548-28268567 Msa0881240:intron 25.0%
!! TGAACATTTAGAATAGTTTG+GGG + chr6_1:28268863-28268882 Msa0881240:intron 25.0%
! TGATTGTCTCAAATTATTGT+CGG - chr6_1:28269080-28269099 None:intergenic 25.0%
! TTTCTATCATATACCATGTT+TGG - chr6_1:28269278-28269297 None:intergenic 25.0%
! AACATTTAGACTATCAAACA+GGG - chr6_1:28269392-28269411 None:intergenic 25.0%
! TACAAATTGTTGTCAAATAG+CGG - chr6_1:28270001-28270020 None:intergenic 25.0%
! CATTATCATTAGTCCAAATT+TGG + chr6_1:28270468-28270487 Msa0881240:intron 25.0%
!!! ACGTCAAAATTATTTTGTGA+AGG - chr6_1:28270546-28270565 None:intergenic 25.0%
!! CAATTAATCTTCATTCGTTT+TGG + chr6_1:28270666-28270685 Msa0881240:intron 25.0%
! TTGAGTTATGACAGTAAATA+AGG + chr6_1:28270919-28270938 Msa0881240:intron 25.0%
! AATACAGCAACACTAATAAT+AGG + chr6_1:28271018-28271037 Msa0881240:intron 25.0%
! ATACAGCAACACTAATAATA+GGG + chr6_1:28271019-28271038 Msa0881240:intron 25.0%
! TCTAGTTATACAATTCAGAA+AGG - chr6_1:28271120-28271139 None:intergenic 25.0%
!! ATGAAATTACATTGATTGGA+TGG + chr6_1:28271140-28271159 Msa0881240:intron 25.0%
! GGATGGTAACAAAAAATTTA+CGG + chr6_1:28271157-28271176 Msa0881240:intron 25.0%
! CAATAATCTTCCCAAAAAAT+TGG - chr6_1:28271480-28271499 None:intergenic 25.0%
! TACAATTCTCGAATAGTATA+TGG - chr6_1:28271505-28271524 None:intergenic 25.0%
! AGAATAATTATGGAATTTGG+AGG + chr6_1:28271552-28271571 Msa0881240:three_prime_UTR 25.0%
! GTTATAATGCGAGTAAAATA+TGG + chr6_1:28271603-28271622 Msa0881240:three_prime_UTR 25.0%
! GAGCAAATAAGATTTATATC+AGG - chr6_1:28271660-28271679 None:intergenic 25.0%
!! GATATAAATCTTATTTGCTC+TGG + chr6_1:28271660-28271679 Msa0881240:three_prime_UTR 25.0%
ATCAGTGTGATTCATTGTAT+CGG + chr6_1:28268193-28268212 Msa0881240:five_prime_UTR 30.0%
GATTCATTGTATCGGAAAAA+AGG + chr6_1:28268201-28268220 Msa0881240:five_prime_UTR 30.0%
TTTCAATTTCAGTTCGTTTG+GGG + chr6_1:28268549-28268568 Msa0881240:intron 30.0%
! CTTTATGCGTGACTTAATTT+TGG + chr6_1:28268763-28268782 Msa0881240:intron 30.0%
! CTCATTTAGAGTTAAGAAAC+CGG + chr6_1:28268805-28268824 Msa0881240:intron 30.0%
! TTTTGTGTAGGATTGCAAAA+TGG + chr6_1:28268837-28268856 Msa0881240:intron 30.0%
TTTGTGTAGGATTGCAAAAT+GGG + chr6_1:28268838-28268857 Msa0881240:intron 30.0%
! GAACATTTAGAATAGTTTGG+GGG + chr6_1:28268864-28268883 Msa0881240:intron 30.0%
TACAACATCTTTCCTACTAT+AGG - chr6_1:28268965-28268984 None:intergenic 30.0%
AAGATGTTGTAACTAAGCTT+AGG + chr6_1:28268974-28268993 Msa0881240:intron 30.0%
! TAATGAGTTGAATCCAAACA+TGG + chr6_1:28269262-28269281 Msa0881240:intron 30.0%
CAACATTTAGACTATCAAAC+AGG - chr6_1:28269393-28269412 None:intergenic 30.0%
ATAGTCTAAATGTTGTCAAG+TGG + chr6_1:28269398-28269417 Msa0881240:intron 30.0%
TATGCTATAAGGGCTTAATT+AGG - chr6_1:28269428-28269447 None:intergenic 30.0%
AATTAAGCCCTTATAGCATA+AGG + chr6_1:28269428-28269447 Msa0881240:intron 30.0%
ATTAAGCCCTTATAGCATAA+GGG + chr6_1:28269429-28269448 Msa0881240:intron 30.0%
AAGTTATCTTGGAGAGTTTA+TGG + chr6_1:28269563-28269582 Msa0881240:intron 30.0%
GCTTATGAACATATCAGTAA+CGG + chr6_1:28269602-28269621 Msa0881240:intron 30.0%
! GCTAATTTGAAATTATCTGC+TGG - chr6_1:28269670-28269689 None:intergenic 30.0%
TCAAATTAGCCAATTCAAAC+AGG + chr6_1:28269680-28269699 Msa0881240:intron 30.0%
AAATGCAAAAGATTAGGGTT+AGG - chr6_1:28269706-28269725 None:intergenic 30.0%
! AACCCTAATCTTTTGCATTT+TGG + chr6_1:28269706-28269725 Msa0881240:intron 30.0%
! ACCCTAATCTTTTGCATTTT+GGG + chr6_1:28269707-28269726 Msa0881240:intron 30.0%
TCCCAAAATGCAAAAGATTA+GGG - chr6_1:28269711-28269730 None:intergenic 30.0%
ATCCCAAAATGCAAAAGATT+AGG - chr6_1:28269712-28269731 None:intergenic 30.0%
!! ACTTGTTAACTTTTGCTTTG+TGG + chr6_1:28269815-28269834 Msa0881240:intron 30.0%
! CTTGTTAACTTTTGCTTTGT+GGG + chr6_1:28269816-28269835 Msa0881240:intron 30.0%
TTGTATTAAATAGCGTTTCG+TGG + chr6_1:28270016-28270035 Msa0881240:intron 30.0%
AGATGTCTCGGAAAAATTAT+GGG - chr6_1:28270150-28270169 None:intergenic 30.0%
TAGATGTCTCGGAAAAATTA+TGG - chr6_1:28270151-28270170 None:intergenic 30.0%
AAACAGTCATTTAGATGTCT+CGG - chr6_1:28270162-28270181 None:intergenic 30.0%
! AAAAAAAGTGCAAACTGAAC+AGG + chr6_1:28270380-28270399 Msa0881240:intron 30.0%
! GAATTTTTCTGATGCTAAAC+TGG + chr6_1:28270426-28270445 Msa0881240:intron 30.0%
ATCATTAGTCCAAATTTGGT+TGG + chr6_1:28270472-28270491 Msa0881240:intron 30.0%
TCAGAATAGAATCACAACTT+AGG + chr6_1:28270504-28270523 Msa0881240:intron 30.0%
!! TTATTTTGTGAAGGAATCCA+AGG - chr6_1:28270537-28270556 None:intergenic 30.0%
! TTAGACTTTAGAGAAAGTAG+TGG + chr6_1:28270704-28270723 Msa0881240:intron 30.0%
TTCATTATAAGATGCCAGTT+TGG + chr6_1:28270759-28270778 Msa0881240:intron 30.0%
!! TATAATGGCATTGACTTCAA+AGG - chr6_1:28270814-28270833 None:intergenic 30.0%
CAAGCCAAAATGATACTAAA+AGG + chr6_1:28270948-28270967 Msa0881240:intron 30.0%
! AGCACCTTTTAGTATCATTT+TGG - chr6_1:28270955-28270974 None:intergenic 30.0%
! TGCTCGTTCTTTGTTAATTT+TGG + chr6_1:28270971-28270990 Msa0881240:intron 30.0%
ACTAATAATAGGGAGAAGTA+TGG + chr6_1:28271029-28271048 Msa0881240:intron 30.0%
! CTAATAATAGGGAGAAGTAT+GGG + chr6_1:28271030-28271049 Msa0881240:intron 30.0%
TACAATTCAGAAAGGAAACA+TGG - chr6_1:28271112-28271131 None:intergenic 30.0%
TGGTCAATTGCAAATTTACA+AGG - chr6_1:28271202-28271221 None:intergenic 30.0%
TTGACTAAAAAAACAGTCGA+TGG - chr6_1:28271236-28271255 None:intergenic 30.0%
TGATCTGGATATTGTCATTA+AGG - chr6_1:28271283-28271302 None:intergenic 30.0%
TGATTATACAGAGCATGTAT+TGG + chr6_1:28271327-28271346 Msa0881240:exon 30.0%
AATAATTTATTGAGGACCTG+AGG - chr6_1:28271382-28271401 None:intergenic 30.0%
TTTACTCGCATTATAACACT+TGG - chr6_1:28271600-28271619 None:intergenic 30.0%
CGAGTAAAATATGGTCTAAT+TGG + chr6_1:28271612-28271631 Msa0881240:three_prime_UTR 30.0%
AGAAAACGTTACATGTTAGC+AGG - chr6_1:28268302-28268321 None:intergenic 35.0%
ATTGTTCTTCCTTCTTGCAA+CGG - chr6_1:28268399-28268418 None:intergenic 35.0%
GAGCATATATGAGAGGAAAA+AGG - chr6_1:28268439-28268458 None:intergenic 35.0%
AACAAACGATCTCTCAAAAC+AGG - chr6_1:28268587-28268606 None:intergenic 35.0%
! TAGTATTTCTGTGCAACTTG+TGG + chr6_1:28268736-28268755 Msa0881240:intron 35.0%
CTACACAAAACACTAAACAC+CGG - chr6_1:28268827-28268846 None:intergenic 35.0%
ATGTTGTAACTAAGCTTAGG+TGG + chr6_1:28268977-28268996 Msa0881240:intron 35.0%
TAACTAAGCTTAGGTGGTTT+GGG + chr6_1:28268983-28269002 Msa0881240:intron 35.0%
GTAGTTATATTTGTCCGTGT+CGG + chr6_1:28269111-28269130 Msa0881240:intron 35.0%
GATAAGCCCTTATGCTATAA+GGG - chr6_1:28269438-28269457 None:intergenic 35.0%
TGATAAGCCCTTATGCTATA+AGG - chr6_1:28269439-28269458 None:intergenic 35.0%
AAGACTGCTTAGACAGTTTA+TGG - chr6_1:28269631-28269650 None:intergenic 35.0%
CAGAGAAGCAATTCAAACTT+TGG - chr6_1:28269769-28269788 None:intergenic 35.0%
AAGTTTGAATTGCTTCTCTG+AGG + chr6_1:28269769-28269788 Msa0881240:intron 35.0%
GAAAGGGGAAATAACATGAT+AGG + chr6_1:28269857-28269876 Msa0881240:intron 35.0%
AGTCTATGTATCCTTGATCT+CGG + chr6_1:28269918-28269937 Msa0881240:intron 35.0%
TCTCGGAAAAATTATGGGAA+GGG - chr6_1:28270145-28270164 None:intergenic 35.0%
GAGACATCTAAATGACTGTT+TGG + chr6_1:28270161-28270180 Msa0881240:intron 35.0%
CTTAAACTTTCAGTGTGGTT+AGG + chr6_1:28270404-28270423 Msa0881240:intron 35.0%
! TAGTCCAAATTTGGTTGGTA+AGG + chr6_1:28270477-28270496 Msa0881240:intron 35.0%
!! AGTCCAAATTTGGTTGGTAA+GGG + chr6_1:28270478-28270497 Msa0881240:intron 35.0%
! ACTTTAGAGAAAGTAGTGGA+AGG + chr6_1:28270708-28270727 Msa0881240:intron 35.0%
! TGGAAGGTTTAAGATCAGTT+AGG + chr6_1:28270724-28270743 Msa0881240:intron 35.0%
! GGAAGGTTTAAGATCAGTTA+GGG + chr6_1:28270725-28270744 Msa0881240:intron 35.0%
ACTCAATTAGTGTCAGTTTG+TGG - chr6_1:28270905-28270924 None:intergenic 35.0%
GACAAGACAAACATGTAAGA+GGG - chr6_1:28271073-28271092 None:intergenic 35.0%
AGACAAGACAAACATGTAAG+AGG - chr6_1:28271074-28271093 None:intergenic 35.0%
!! AGTCGATGGATTGTTGAATT+TGG - chr6_1:28271222-28271241 None:intergenic 35.0%
AGCATCATACAAATCTGATC+TGG - chr6_1:28271298-28271317 None:intergenic 35.0%
ATTATAACACTTGGAGTTGG+AGG - chr6_1:28271591-28271610 None:intergenic 35.0%
GTTTCAACAACTCTCGATCT+CGG + chr6_1:28268244-28268263 Msa0881240:five_prime_UTR 40.0%
TTGTTCTTCCTTCTTGCAAC+GGG - chr6_1:28268398-28268417 None:intergenic 40.0%
GTTGCAAGAAGGAAGAACAA+TGG + chr6_1:28268398-28268417 Msa0881240:CDS 40.0%
AGAGGAAAAAGGAACTTGAG+AGG - chr6_1:28268428-28268447 None:intergenic 40.0%
! AGAACAGGAGCATATATGAG+AGG - chr6_1:28268446-28268465 None:intergenic 40.0%
AGTGGAGTGACTAACTTAGA+GGG - chr6_1:28268469-28268488 None:intergenic 40.0%
AAGTGGAGTGACTAACTTAG+AGG - chr6_1:28268470-28268489 None:intergenic 40.0%
TCGTTTGTTTACAGCTGTCT+TGG + chr6_1:28268598-28268617 Msa0881240:CDS 40.0%
! CGGTGTTTAGTGTTTTGTGT+AGG + chr6_1:28268825-28268844 Msa0881240:intron 40.0%
GTAACTAAGCTTAGGTGGTT+TGG + chr6_1:28268982-28269001 Msa0881240:intron 40.0%
AGTAAGGAGGGTAGATCATA+TGG + chr6_1:28269035-28269054 Msa0881240:intron 40.0%
TATGAAGCACAAACTCTCCT+TGG - chr6_1:28269173-28269192 None:intergenic 40.0%
AAGGAGAGTTTGTGCTTCAT+AGG + chr6_1:28269172-28269191 Msa0881240:intron 40.0%
AGAGTTTGTGCTTCATAGGA+TGG + chr6_1:28269176-28269195 Msa0881240:intron 40.0%
TGTCTAAGCAGTCTTACAAG+TGG + chr6_1:28269636-28269655 Msa0881240:intron 40.0%
TGTGTGAGTAGACAAGGAAA+GGG + chr6_1:28269841-28269860 Msa0881240:intron 40.0%
TCCTTGATCTCGGAAAATAG+AGG + chr6_1:28269928-28269947 Msa0881240:intron 40.0%
! ACCTCTATTTTCCGAGATCA+AGG - chr6_1:28269932-28269951 None:intergenic 40.0%
CATATGGCATTGTAGCATAG+CGG - chr6_1:28269966-28269985 None:intergenic 40.0%
TTGTCAAATAGCGGCTATAC+TGG - chr6_1:28269992-28270011 None:intergenic 40.0%
TGCGCTATAACGCAATAACA+CGG - chr6_1:28270068-28270087 None:intergenic 40.0%
TGACAACACTAGTCAATGCT+AGG + chr6_1:28270102-28270121 Msa0881240:intron 40.0%
CAATGCTAGGCAAGAAAAGT+AGG + chr6_1:28270115-28270134 Msa0881240:intron 40.0%
GTCTCGGAAAAATTATGGGA+AGG - chr6_1:28270146-28270165 None:intergenic 40.0%
CAGGTCTTAAACTTTCAGTG+TGG + chr6_1:28270399-28270418 Msa0881240:intron 40.0%
!! GTCCAAATTTGGTTGGTAAG+GGG + chr6_1:28270479-28270498 Msa0881240:intron 40.0%
!! TCCAAATTTGGTTGGTAAGG+GGG + chr6_1:28270480-28270499 Msa0881240:intron 40.0%
ACAACTTAGGCATCTTTCCT+TGG + chr6_1:28270517-28270536 Msa0881240:intron 40.0%
!! TAAGATCAGTTAGGGTGTTG+AGG + chr6_1:28270733-28270752 Msa0881240:intron 40.0%
TAAGAGCAAGAATGCCAAAC+TGG - chr6_1:28270776-28270795 None:intergenic 40.0%
!! TGGCATTGACTTCAAAGGAA+TGG - chr6_1:28270809-28270828 None:intergenic 40.0%
ATCGTTTACATCACAACCTC+AGG + chr6_1:28271363-28271382 Msa0881240:three_prime_UTR 40.0%
!! TCTCTATTTAAGGTGTTGCC+AGG - chr6_1:28271429-28271448 None:intergenic 40.0%
CGCATTATAACACTTGGAGT+TGG - chr6_1:28271594-28271613 None:intergenic 40.0%
CGAGAGTTGTTGAAACAGCA+GGG - chr6_1:28268240-28268259 None:intergenic 45.0%
TCGAGAGTTGTTGAAACAGC+AGG - chr6_1:28268241-28268260 None:intergenic 45.0%
GGAAAAAGGAACTTGAGAGG+AGG - chr6_1:28268425-28268444 None:intergenic 45.0%
GACTAACTTAGAGGGAGAAC+AGG - chr6_1:28268461-28268480 None:intergenic 45.0%
! TTTCAGTTCGTTTGGGGTTG+CGG + chr6_1:28268555-28268574 Msa0881240:intron 45.0%
TTGTTTACAGCTGTCTTGGC+TGG + chr6_1:28268602-28268621 Msa0881240:CDS 45.0%
! TTGGCTGGTGCTTTTCTTCA+TGG + chr6_1:28268617-28268636 Msa0881240:CDS 45.0%
AGAGAGAGAGAGAGAGAGTT+GGG + chr6_1:28268927-28268946 Msa0881240:intron 45.0%
AAGGAGGGTAGATCATATGG+AGG + chr6_1:28269038-28269057 Msa0881240:intron 45.0%
GCACAAACTCTCCTTGGATT+AGG - chr6_1:28269167-28269186 None:intergenic 45.0%
GTTTGTGCTTCATAGGATGG+AGG + chr6_1:28269179-28269198 Msa0881240:intron 45.0%
ACTAAGTGGGGTCAGTTACA+TGG - chr6_1:28269319-28269338 None:intergenic 45.0%
ATGTAACTGACCCCACTTAG+TGG + chr6_1:28269318-28269337 Msa0881240:intron 45.0%
TGTAACTGACCCCACTTAGT+GGG + chr6_1:28269319-28269338 Msa0881240:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr6_1:28269331-28269350 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr6_1:28269332-28269351 None:intergenic 45.0%
! TCAAGCCTTATCCCACTAAG+TGG - chr6_1:28269333-28269352 None:intergenic 45.0%
AGGGTTAGGCCTGTTTGAAT+TGG - chr6_1:28269692-28269711 None:intergenic 45.0%
!! AATTGCTTCTCTGAGGCTAC+TGG + chr6_1:28269776-28269795 Msa0881240:intron 45.0%
CTGTGTGAGTAGACAAGGAA+AGG + chr6_1:28269840-28269859 Msa0881240:intron 45.0%
GTGTGAGTAGACAAGGAAAG+GGG + chr6_1:28269842-28269861 Msa0881240:intron 45.0%
CGCTATGCTACAATGCCATA+TGG + chr6_1:28269964-28269983 Msa0881240:intron 45.0%
GCTATAACGCAATAACACGG+CGG - chr6_1:28270065-28270084 None:intergenic 45.0%
GGTTGTGATGTAAACGATGC+TGG - chr6_1:28271361-28271380 None:intergenic 45.0%
! GTGTTTGCTGCTCAAAATCC+TGG + chr6_1:28271408-28271427 Msa0881240:three_prime_UTR 45.0%
TTAGCAGGATCGAACGACGT+CGG - chr6_1:28268287-28268306 None:intergenic 50.0%
ACAGAGAACCCGTTGCAAGA+AGG + chr6_1:28268387-28268406 Msa0881240:intron 50.0%
! GCTGGTGCTTTTCTTCATGG+AGG + chr6_1:28268620-28268639 Msa0881240:CDS 50.0%
GAGAGAGAGAGAGAGAGAGT+TGG + chr6_1:28268926-28268945 Msa0881240:intron 50.0%
GAGAGAGAGAGAGAGAGTTG+GGG + chr6_1:28268928-28268947 Msa0881240:intron 50.0%
AGAGAGAGAGAGTTGGGGTT+GGG + chr6_1:28268933-28268952 Msa0881240:intron 50.0%
GCATGTAGAGAGAAGACACG+TGG + chr6_1:28269005-28269024 Msa0881240:intron 50.0%
GACACGTGGATTCTGCAGTA+AGG + chr6_1:28269019-28269038 Msa0881240:intron 50.0%
ACGTGGATTCTGCAGTAAGG+AGG + chr6_1:28269022-28269041 Msa0881240:intron 50.0%
CGTGGATTCTGCAGTAAGGA+GGG + chr6_1:28269023-28269042 Msa0881240:intron 50.0%
GATTAGGCATGTGCCAGTGT+CGG - chr6_1:28269151-28269170 None:intergenic 50.0%
ACTGGCACATGCCTAATCCA+AGG + chr6_1:28269153-28269172 Msa0881240:intron 50.0%
TGACCCCACTTAGTGGGATA+AGG + chr6_1:28269325-28269344 Msa0881240:intron 50.0%
!! TCTCTGAGGCTACTGGAGAT+TGG + chr6_1:28269783-28269802 Msa0881240:intron 50.0%
TGGGTCTGTGTGAGTAGACA+AGG + chr6_1:28269835-28269854 Msa0881240:intron 50.0%
GCCCCCTTACCAACCAAATT+TGG - chr6_1:28270484-28270503 None:intergenic 50.0%
GATCTCGGTCAATTCCGCCA+TGG + chr6_1:28268259-28268278 Msa0881240:exon 55.0%
GAGAGAGAGAGAGTTGGGGT+TGG + chr6_1:28268932-28268951 Msa0881240:intron 55.0%
GAGAGAGAGAGTTGGGGTTG+GGG + chr6_1:28268934-28268953 Msa0881240:intron 55.0%
GTTGGGGTGGCACCTATAGT+AGG + chr6_1:28268950-28268969 Msa0881240:intron 55.0%
GCGGCTATACTGGCACCATA+TGG - chr6_1:28269982-28270001 None:intergenic 55.0%
AGAGAGAGTTGGGGTTGGGG+TGG + chr6_1:28268937-28268956 Msa0881240:intron 60.0%
ATCGAACGACGTCGGCGCCA+TGG - chr6_1:28268279-28268298 None:intergenic 65.0%
! ACACGCGTCGGTGTCCGACA+CGG - chr6_1:28269128-28269147 None:intergenic 65.0%
! TGCCAGTGTCGGACACGCGT+CGG - chr6_1:28269140-28269159 None:intergenic 65.0%
GAACGACGTCGGCGCCATGG+CGG - chr6_1:28268276-28268295 None:intergenic 70.0%
CACCGACGCGTGTCCGACAC+TGG + chr6_1:28269135-28269154 Msa0881240:intron 70.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 28268173 28271752 28268173 ID=Msa0881240;Name=Msa0881240
chr6_1 mRNA 28268173 28271752 28268173 ID=Msa0881240-mRNA-1;Parent=Msa0881240;Name=Msa0881240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|1|1|1|0.66|0.75|4|423|78
chr6_1 exon 28268173 28268309 28268173 ID=Msa0881240-mRNA-1:exon:7095;Parent=Msa0881240-mRNA-1
chr6_1 exon 28268391 28268473 28268391 ID=Msa0881240-mRNA-1:exon:7096;Parent=Msa0881240-mRNA-1
chr6_1 exon 28268561 28268651 28268561 ID=Msa0881240-mRNA-1:exon:7097;Parent=Msa0881240-mRNA-1
chr6_1 exon 28271299 28271752 28271299 ID=Msa0881240-mRNA-1:exon:7098;Parent=Msa0881240-mRNA-1
chr6_1 five_prime_UTR 28268173 28268277 28268173 ID=Msa0881240-mRNA-1:five_prime_utr;Parent=Msa0881240-mRNA-1
chr6_1 CDS 28268278 28268309 28268278 ID=Msa0881240-mRNA-1:cds;Parent=Msa0881240-mRNA-1
chr6_1 CDS 28268391 28268473 28268391 ID=Msa0881240-mRNA-1:cds;Parent=Msa0881240-mRNA-1
chr6_1 CDS 28268561 28268651 28268561 ID=Msa0881240-mRNA-1:cds;Parent=Msa0881240-mRNA-1
chr6_1 CDS 28271299 28271329 28271299 ID=Msa0881240-mRNA-1:cds;Parent=Msa0881240-mRNA-1
chr6_1 three_prime_UTR 28271330 28271752 28271330 ID=Msa0881240-mRNA-1:three_prime_utr;Parent=Msa0881240-mRNA-1
Gene Sequence

>Msa0881240

ATGGCGCCGACGTCGTTCGATCCTGCTAACATAGAACCCGTTGCAAGAAGGAAGAACAATGGTCCTCCTCTCAAGTTCCTTTTTCCTCTCATATATGCTCCTGTTCTCCCTCTAATTCGTTTGGGGTTGCGGCATAAACCTGTTTTGAGAGATCGTTTGTTTACAGCTGTCTTGGCTGGTGCTTTTCTTCATGGAGGCTATCTTGTATCAGATTTGTATGATGCTGAGAGCAAATGA
Protein sequence

>Msa0881240

MAPTSFDPANIEPVARRKNNGPPLKFLFPLIYAPVLPLIRLGLRHKPVLRDRLFTAVLAGAFLHGGYLVSDLYDAESK*