AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0563560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563560 MtrunA17_Chr8g0377361 100.000 74 0 0 1 74 1 74 2.96e-53 160
Msa0563560 MtrunA17_Chr3g0095021 90.000 50 5 0 1 50 53 102 3.26e-29 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563560 AT1G67350.2 74.324 74 19 0 1 74 1 74 2.85e-39 125
Msa0563560 AT1G67350.1 74.324 74 19 0 1 74 1 74 2.85e-39 125

Find 40 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTAAGATTTAACTGTTC+TGG 0.229897 4_1:+63936675 Msa0563560:three_prime_UTR
TAGAACTATGGTGTTATATT+AGG 0.246291 4_1:+63936536 Msa0563560:three_prime_UTR
AGTTACCCTATGCGACCTTA+TGG 0.310546 4_1:+63935180 Msa0563560:CDS
GGGTCTGTAATGAACTATTT+TGG 0.311085 4_1:+63936494 Msa0563560:three_prime_UTR
AATCTCTTTATTTACATAGT+TGG 0.340518 4_1:+63936631 Msa0563560:three_prime_UTR
AATCTACGATCCCTTTCATT+TGG 0.347988 4_1:-63935102 None:intergenic
CTATGCGACCTTATGGGTTC+TGG 0.350279 4_1:+63935187 Msa0563560:CDS
AGTGAAAATGGCTTTCATAA+TGG 0.354869 4_1:+63935041 None:intergenic
TAGTGGCCAATTGTGCTTCC+TGG 0.360865 4_1:+63936583 Msa0563560:three_prime_UTR
ATGAAAGGGATCGTAGATTC+AGG 0.366060 4_1:+63935106 Msa0563560:CDS
AATAAAGTTTCATAGAACTA+TGG 0.369039 4_1:+63936524 Msa0563560:three_prime_UTR
ATCTCTTTATTTACATAGTT+GGG 0.371188 4_1:+63936632 Msa0563560:three_prime_UTR
AATGGAATTCGCTGAGAATC+TGG 0.378487 4_1:+63935059 Msa0563560:CDS
ATATTAGGTGTTTCTCCATT+GGG 0.409394 4_1:+63936551 Msa0563560:three_prime_UTR
AAACAGCCAGGAAGCACAAT+TGG 0.424037 4_1:-63936589 None:intergenic
GTTACCCTATGCGACCTTAT+GGG 0.431260 4_1:+63935181 Msa0563560:CDS
TATATTAGGTGTTTCTCCAT+TGG 0.438705 4_1:+63936550 Msa0563560:three_prime_UTR
GAATCTGGTTCTGAAATTGA+TGG 0.457082 4_1:+63935074 Msa0563560:CDS
CCATTGGGAATATAAATTAG+TGG 0.458740 4_1:+63936566 Msa0563560:three_prime_UTR
CCCAAGCAAGCTGAGAGTTA+TGG 0.460911 4_1:-63935221 None:intergenic
GCCATAACTCTCAGCTTGCT+TGG 0.460971 4_1:+63935220 Msa0563560:CDS
CCATAACTCTCAGCTTGCTT+GGG 0.463504 4_1:+63935221 Msa0563560:CDS
GCTCAGATTAGTCAAGTTGC+TGG 0.473187 4_1:+63935246 Msa0563560:CDS
AATGTAACATGCAACAGAAC+AGG 0.481138 4_1:-63936462 None:intergenic
CCACTAATTTATATTCCCAA+TGG 0.511619 4_1:-63936566 None:intergenic
TCAAATGTCCAGAACCCATA+AGG 0.513007 4_1:-63935195 None:intergenic
GATGGAAGATCCAAATGAAA+GGG 0.524216 4_1:+63935092 Msa0563560:CDS
CATGTTACATTGACTACACC+GGG 0.527959 4_1:+63936474 Msa0563560:three_prime_UTR
TGATGGAAGATCCAAATGAA+AGG 0.535018 4_1:+63935091 Msa0563560:CDS
CATAAAACAGACAAACAGCC+AGG 0.549333 4_1:-63936601 None:intergenic
TGAAAGGGATCGTAGATTCA+GGG 0.568833 4_1:+63935107 Msa0563560:CDS
CAGAACCCATAAGGTCGCAT+AGG 0.571457 4_1:-63935186 None:intergenic
GCGCGAAGACGAAGGAGATG+TGG 0.578349 4_1:+63935157 Msa0563560:CDS
GCATGTTACATTGACTACAC+CGG 0.580850 4_1:+63936473 Msa0563560:three_prime_UTR
AGAACCCATAAGGTCGCATA+GGG 0.582926 4_1:-63935185 None:intergenic
GGATAGGTGCGCGAAGACGA+AGG 0.611249 4_1:+63935149 Msa0563560:CDS
ACGTGTATAAGACGAAGGAT+AGG 0.624854 4_1:+63935133 Msa0563560:CDS
AAAATAGTTCATTACAGACC+CGG 0.668711 4_1:-63936492 None:intergenic
CTTGTGAGTTAAGACAACTT+CGG 0.696108 4_1:-63936371 None:intergenic
GGAACACGTGTATAAGACGA+AGG 0.707448 4_1:+63935128 Msa0563560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATAAAAATAACATGTTA+GGG - chr4_1:63935348-63935367 None:intergenic 15.0%
!! TCAAATAAAAATAACATGTT+AGG - chr4_1:63935349-63935368 None:intergenic 15.0%
!!! AACATGTTATTTTTATTTGA+TGG + chr4_1:63935349-63935368 Msa0563560:intron 15.0%
!!! AATTAGTAATAATTTGTTAG+AGG + chr4_1:63935676-63935695 Msa0563560:intron 15.0%
!!! ATTAGTAATAATTTGTTAGA+GGG + chr4_1:63935677-63935696 Msa0563560:intron 15.0%
!! TATAAATTAAGGAAAAAGAT+GGG - chr4_1:63936213-63936232 None:intergenic 15.0%
!! TTATAAATTAAGGAAAAAGA+TGG - chr4_1:63936214-63936233 None:intergenic 15.0%
!! TGAAAGAGATTTATAAATTA+AGG - chr4_1:63936224-63936243 None:intergenic 15.0%
!!! ATTTAATTCAATTTTTGTCT+TGG + chr4_1:63936707-63936726 Msa0563560:three_prime_UTR 15.0%
!! CAAATAAAAATAACATGTTA+GGG - chr4_1:63935348-63935367 None:intergenic 15.0%
!! TCAAATAAAAATAACATGTT+AGG - chr4_1:63935349-63935368 None:intergenic 15.0%
!!! AACATGTTATTTTTATTTGA+TGG + chr4_1:63935349-63935368 Msa0563560:intron 15.0%
!!! AATTAGTAATAATTTGTTAG+AGG + chr4_1:63935676-63935695 Msa0563560:intron 15.0%
!!! ATTAGTAATAATTTGTTAGA+GGG + chr4_1:63935677-63935696 Msa0563560:intron 15.0%
!! TATAAATTAAGGAAAAAGAT+GGG - chr4_1:63936213-63936232 None:intergenic 15.0%
!! TTATAAATTAAGGAAAAAGA+TGG - chr4_1:63936214-63936233 None:intergenic 15.0%
!! TGAAAGAGATTTATAAATTA+AGG - chr4_1:63936224-63936243 None:intergenic 15.0%
!! AAAGATTAAGTTTCTCATAA+AGG - chr4_1:63935799-63935818 None:intergenic 20.0%
!! AATAAAGTTTCATAGAACTA+TGG + chr4_1:63936524-63936543 Msa0563560:three_prime_UTR 20.0%
!!! AATCTCTTTATTTACATAGT+TGG + chr4_1:63936631-63936650 Msa0563560:three_prime_UTR 20.0%
!!! ATCTCTTTATTTACATAGTT+GGG + chr4_1:63936632-63936651 Msa0563560:three_prime_UTR 20.0%
!! AAAGATTAAGTTTCTCATAA+AGG - chr4_1:63935799-63935818 None:intergenic 20.0%
!! AATAAAGTTTCATAGAACTA+TGG + chr4_1:63936524-63936543 Msa0563560:three_prime_UTR 20.0%
!!! AATCTCTTTATTTACATAGT+TGG + chr4_1:63936631-63936650 Msa0563560:three_prime_UTR 20.0%
!!! ATCTCTTTATTTACATAGTT+GGG + chr4_1:63936632-63936651 Msa0563560:three_prime_UTR 20.0%
!! TTTATTTGATGGCTATATGT+TGG + chr4_1:63935360-63935379 Msa0563560:intron 25.0%
! GTTTATCATAGAGTAAATGA+CGG - chr4_1:63935628-63935647 None:intergenic 25.0%
! GAGAAACTTAATCTTTAATG+TGG + chr4_1:63935803-63935822 Msa0563560:intron 25.0%
! ATAACCGTATTTAACAACAA+TGG + chr4_1:63936010-63936029 Msa0563560:intron 25.0%
! CATCAGTTAATAGGAAAAAA+AGG - chr4_1:63936078-63936097 None:intergenic 25.0%
! CATGTTTATCATCAGTTAAT+AGG - chr4_1:63936087-63936106 None:intergenic 25.0%
! TAACTGATGATAAACATGTA+TGG + chr4_1:63936089-63936108 Msa0563560:intron 25.0%
!!! GTTTTCAAGCAATACAATAA+AGG - chr4_1:63936140-63936159 None:intergenic 25.0%
! TGAAAAACTGGAACTATATT+GGG + chr4_1:63936403-63936422 Msa0563560:three_prime_UTR 25.0%
!!! TAGAACTATGGTGTTATATT+AGG + chr4_1:63936536-63936555 Msa0563560:three_prime_UTR 25.0%
!! TGTTTAAGATTTAACTGTTC+TGG + chr4_1:63936675-63936694 Msa0563560:three_prime_UTR 25.0%
!! TTTATTTGATGGCTATATGT+TGG + chr4_1:63935360-63935379 Msa0563560:intron 25.0%
! GTTTATCATAGAGTAAATGA+CGG - chr4_1:63935628-63935647 None:intergenic 25.0%
! GAGAAACTTAATCTTTAATG+TGG + chr4_1:63935803-63935822 Msa0563560:intron 25.0%
! ATAACCGTATTTAACAACAA+TGG + chr4_1:63936010-63936029 Msa0563560:intron 25.0%
! CATCAGTTAATAGGAAAAAA+AGG - chr4_1:63936078-63936097 None:intergenic 25.0%
! CATGTTTATCATCAGTTAAT+AGG - chr4_1:63936087-63936106 None:intergenic 25.0%
! TAACTGATGATAAACATGTA+TGG + chr4_1:63936089-63936108 Msa0563560:intron 25.0%
!!! GTTTTCAAGCAATACAATAA+AGG - chr4_1:63936140-63936159 None:intergenic 25.0%
! TGAAAAACTGGAACTATATT+GGG + chr4_1:63936403-63936422 Msa0563560:three_prime_UTR 25.0%
!!! TAGAACTATGGTGTTATATT+AGG + chr4_1:63936536-63936555 Msa0563560:three_prime_UTR 25.0%
!! TGTTTAAGATTTAACTGTTC+TGG + chr4_1:63936675-63936694 Msa0563560:three_prime_UTR 25.0%
TGCGAAAGAATTTCATCATA+AGG - chr4_1:63935282-63935301 None:intergenic 30.0%
!! AGTAATAATTTGTTAGAGGG+AGG + chr4_1:63935680-63935699 Msa0563560:intron 30.0%
!! GTAATAATTTGTTAGAGGGA+GGG + chr4_1:63935681-63935700 Msa0563560:intron 30.0%
!! TAATAATTTGTTAGAGGGAG+GGG + chr4_1:63935682-63935701 Msa0563560:intron 30.0%
GTTGTTAAATACGGTTATAG+TGG - chr4_1:63936008-63936027 None:intergenic 30.0%
! TCAGCCATTGTTGTTAAATA+CGG - chr4_1:63936017-63936036 None:intergenic 30.0%
AAGAAAACAATGTTGAACTC+CGG - chr4_1:63936044-63936063 None:intergenic 30.0%
AATGCATTGCATGATATGAT+AGG - chr4_1:63936171-63936190 None:intergenic 30.0%
AAAAAGATGGGAAAAAATGG+TGG - chr4_1:63936201-63936220 None:intergenic 30.0%
AGGAAAAAGATGGGAAAAAA+TGG - chr4_1:63936204-63936223 None:intergenic 30.0%
AAGTGTTCTTTGTGAAAAAC+TGG + chr4_1:63936391-63936410 Msa0563560:three_prime_UTR 30.0%
GTGAAAAACTGGAACTATAT+TGG + chr4_1:63936402-63936421 Msa0563560:three_prime_UTR 30.0%
AAAATAGTTCATTACAGACC+CGG - chr4_1:63936495-63936514 None:intergenic 30.0%
TATATTAGGTGTTTCTCCAT+TGG + chr4_1:63936550-63936569 Msa0563560:three_prime_UTR 30.0%
ATATTAGGTGTTTCTCCATT+GGG + chr4_1:63936551-63936570 Msa0563560:three_prime_UTR 30.0%
CCACTAATTTATATTCCCAA+TGG - chr4_1:63936569-63936588 None:intergenic 30.0%
CCATTGGGAATATAAATTAG+TGG + chr4_1:63936566-63936585 Msa0563560:three_prime_UTR 30.0%
TGCGAAAGAATTTCATCATA+AGG - chr4_1:63935282-63935301 None:intergenic 30.0%
!! AGTAATAATTTGTTAGAGGG+AGG + chr4_1:63935680-63935699 Msa0563560:intron 30.0%
!! GTAATAATTTGTTAGAGGGA+GGG + chr4_1:63935681-63935700 Msa0563560:intron 30.0%
!! TAATAATTTGTTAGAGGGAG+GGG + chr4_1:63935682-63935701 Msa0563560:intron 30.0%
GTTGTTAAATACGGTTATAG+TGG - chr4_1:63936008-63936027 None:intergenic 30.0%
! TCAGCCATTGTTGTTAAATA+CGG - chr4_1:63936017-63936036 None:intergenic 30.0%
AAGAAAACAATGTTGAACTC+CGG - chr4_1:63936044-63936063 None:intergenic 30.0%
AATGCATTGCATGATATGAT+AGG - chr4_1:63936171-63936190 None:intergenic 30.0%
AAAAAGATGGGAAAAAATGG+TGG - chr4_1:63936201-63936220 None:intergenic 30.0%
AGGAAAAAGATGGGAAAAAA+TGG - chr4_1:63936204-63936223 None:intergenic 30.0%
AAGTGTTCTTTGTGAAAAAC+TGG + chr4_1:63936391-63936410 Msa0563560:three_prime_UTR 30.0%
GTGAAAAACTGGAACTATAT+TGG + chr4_1:63936402-63936421 Msa0563560:three_prime_UTR 30.0%
AAAATAGTTCATTACAGACC+CGG - chr4_1:63936495-63936514 None:intergenic 30.0%
TATATTAGGTGTTTCTCCAT+TGG + chr4_1:63936550-63936569 Msa0563560:three_prime_UTR 30.0%
ATATTAGGTGTTTCTCCATT+GGG + chr4_1:63936551-63936570 Msa0563560:three_prime_UTR 30.0%
CCACTAATTTATATTCCCAA+TGG - chr4_1:63936569-63936588 None:intergenic 30.0%
CCATTGGGAATATAAATTAG+TGG + chr4_1:63936566-63936585 Msa0563560:three_prime_UTR 30.0%
!! GAATCTGGTTCTGAAATTGA+TGG + chr4_1:63935074-63935093 Msa0563560:CDS 35.0%
TGATGGAAGATCCAAATGAA+AGG + chr4_1:63935091-63935110 Msa0563560:CDS 35.0%
GATGGAAGATCCAAATGAAA+GGG + chr4_1:63935092-63935111 Msa0563560:CDS 35.0%
AATCTACGATCCCTTTCATT+TGG - chr4_1:63935105-63935124 None:intergenic 35.0%
TGAAGAGCAAAAACCTCATT+TGG - chr4_1:63935322-63935341 None:intergenic 35.0%
TTGTATGTAACTAGATGCGT+TGG + chr4_1:63935467-63935486 Msa0563560:intron 35.0%
!! AATAATTTGTTAGAGGGAGG+GGG + chr4_1:63935683-63935702 Msa0563560:intron 35.0%
GAATTCAGTGTTGTCAATTG+CGG + chr4_1:63935834-63935853 Msa0563560:intron 35.0%
!! TTCTGAAATAGCAGCTATAG+CGG - chr4_1:63935912-63935931 None:intergenic 35.0%
AGATTGTTGAAAGTTTGACG+AGG + chr4_1:63936249-63936268 Msa0563560:intron 35.0%
ACAGTGCATTAGCAAAACAA+AGG - chr4_1:63936304-63936323 None:intergenic 35.0%
CTTGTGAGTTAAGACAACTT+CGG - chr4_1:63936374-63936393 None:intergenic 35.0%
AATGTAACATGCAACAGAAC+AGG - chr4_1:63936465-63936484 None:intergenic 35.0%
! GGGTCTGTAATGAACTATTT+TGG + chr4_1:63936494-63936513 Msa0563560:three_prime_UTR 35.0%
! TGGCTGTTTGTCTGTTTTAT+GGG + chr4_1:63936603-63936622 Msa0563560:three_prime_UTR 35.0%
!! GAATCTGGTTCTGAAATTGA+TGG + chr4_1:63935074-63935093 Msa0563560:CDS 35.0%
TGATGGAAGATCCAAATGAA+AGG + chr4_1:63935091-63935110 Msa0563560:CDS 35.0%
GATGGAAGATCCAAATGAAA+GGG + chr4_1:63935092-63935111 Msa0563560:CDS 35.0%
AATCTACGATCCCTTTCATT+TGG - chr4_1:63935105-63935124 None:intergenic 35.0%
TGAAGAGCAAAAACCTCATT+TGG - chr4_1:63935322-63935341 None:intergenic 35.0%
TTGTATGTAACTAGATGCGT+TGG + chr4_1:63935467-63935486 Msa0563560:intron 35.0%
!! AATAATTTGTTAGAGGGAGG+GGG + chr4_1:63935683-63935702 Msa0563560:intron 35.0%
GAATTCAGTGTTGTCAATTG+CGG + chr4_1:63935834-63935853 Msa0563560:intron 35.0%
!! TTCTGAAATAGCAGCTATAG+CGG - chr4_1:63935912-63935931 None:intergenic 35.0%
AGATTGTTGAAAGTTTGACG+AGG + chr4_1:63936249-63936268 Msa0563560:intron 35.0%
ACAGTGCATTAGCAAAACAA+AGG - chr4_1:63936304-63936323 None:intergenic 35.0%
CTTGTGAGTTAAGACAACTT+CGG - chr4_1:63936374-63936393 None:intergenic 35.0%
AATGTAACATGCAACAGAAC+AGG - chr4_1:63936465-63936484 None:intergenic 35.0%
! GGGTCTGTAATGAACTATTT+TGG + chr4_1:63936494-63936513 Msa0563560:three_prime_UTR 35.0%
! TGGCTGTTTGTCTGTTTTAT+GGG + chr4_1:63936603-63936622 Msa0563560:three_prime_UTR 35.0%
AATGGAATTCGCTGAGAATC+TGG + chr4_1:63935059-63935078 Msa0563560:CDS 40.0%
ATGAAAGGGATCGTAGATTC+AGG + chr4_1:63935106-63935125 Msa0563560:CDS 40.0%
TGAAAGGGATCGTAGATTCA+GGG + chr4_1:63935107-63935126 Msa0563560:CDS 40.0%
ACGTGTATAAGACGAAGGAT+AGG + chr4_1:63935133-63935152 Msa0563560:CDS 40.0%
TCAAATGTCCAGAACCCATA+AGG - chr4_1:63935198-63935217 None:intergenic 40.0%
AAAACCTCATTTGGACGATG+AGG - chr4_1:63935313-63935332 None:intergenic 40.0%
GCACGTTTGATATGAAGTTC+CGG + chr4_1:63935505-63935524 Msa0563560:intron 40.0%
! ATTGCTAGAAGGGTTTGGTT+GGG + chr4_1:63935534-63935553 Msa0563560:intron 40.0%
TTGGCTAAGATTAGAGTCGA+TGG + chr4_1:63935571-63935590 Msa0563560:intron 40.0%
AGTTGTTTGCAAGAAGTCCA+AGG - chr4_1:63935732-63935751 None:intergenic 40.0%
CTTCTTGCAAACAACTCCTT+TGG + chr4_1:63935736-63935755 Msa0563560:intron 40.0%
! TGTTGTCAATTGCGGATTGT+GGG + chr4_1:63935842-63935861 Msa0563560:intron 40.0%
GTTGCATTGCGGATTTGAAA+AGG - chr4_1:63935877-63935896 None:intergenic 40.0%
GGCGCTATAACACTATTGAA+TGG - chr4_1:63935987-63936006 None:intergenic 40.0%
AACAACAATGGCTGAGTTAC+CGG + chr4_1:63936022-63936041 Msa0563560:intron 40.0%
TTGTTGAAAGTTTGACGAGG+AGG + chr4_1:63936252-63936271 Msa0563560:intron 40.0%
TGTTGAAAGTTTGACGAGGA+GGG + chr4_1:63936253-63936272 Msa0563560:intron 40.0%
! GCATGTTACATTGACTACAC+CGG + chr4_1:63936473-63936492 Msa0563560:three_prime_UTR 40.0%
! CATGTTACATTGACTACACC+GGG + chr4_1:63936474-63936493 Msa0563560:three_prime_UTR 40.0%
CATAAAACAGACAAACAGCC+AGG - chr4_1:63936604-63936623 None:intergenic 40.0%
! CTGGCTGTTTGTCTGTTTTA+TGG + chr4_1:63936602-63936621 Msa0563560:three_prime_UTR 40.0%
AATGGAATTCGCTGAGAATC+TGG + chr4_1:63935059-63935078 Msa0563560:CDS 40.0%
ATGAAAGGGATCGTAGATTC+AGG + chr4_1:63935106-63935125 Msa0563560:CDS 40.0%
TGAAAGGGATCGTAGATTCA+GGG + chr4_1:63935107-63935126 Msa0563560:CDS 40.0%
ACGTGTATAAGACGAAGGAT+AGG + chr4_1:63935133-63935152 Msa0563560:CDS 40.0%
TCAAATGTCCAGAACCCATA+AGG - chr4_1:63935198-63935217 None:intergenic 40.0%
AAAACCTCATTTGGACGATG+AGG - chr4_1:63935313-63935332 None:intergenic 40.0%
GCACGTTTGATATGAAGTTC+CGG + chr4_1:63935505-63935524 Msa0563560:intron 40.0%
! ATTGCTAGAAGGGTTTGGTT+GGG + chr4_1:63935534-63935553 Msa0563560:intron 40.0%
TTGGCTAAGATTAGAGTCGA+TGG + chr4_1:63935571-63935590 Msa0563560:intron 40.0%
AGTTGTTTGCAAGAAGTCCA+AGG - chr4_1:63935732-63935751 None:intergenic 40.0%
CTTCTTGCAAACAACTCCTT+TGG + chr4_1:63935736-63935755 Msa0563560:intron 40.0%
! TGTTGTCAATTGCGGATTGT+GGG + chr4_1:63935842-63935861 Msa0563560:intron 40.0%
GTTGCATTGCGGATTTGAAA+AGG - chr4_1:63935877-63935896 None:intergenic 40.0%
GGCGCTATAACACTATTGAA+TGG - chr4_1:63935987-63936006 None:intergenic 40.0%
AACAACAATGGCTGAGTTAC+CGG + chr4_1:63936022-63936041 Msa0563560:intron 40.0%
TTGTTGAAAGTTTGACGAGG+AGG + chr4_1:63936252-63936271 Msa0563560:intron 40.0%
TGTTGAAAGTTTGACGAGGA+GGG + chr4_1:63936253-63936272 Msa0563560:intron 40.0%
! GCATGTTACATTGACTACAC+CGG + chr4_1:63936473-63936492 Msa0563560:three_prime_UTR 40.0%
! CATGTTACATTGACTACACC+GGG + chr4_1:63936474-63936493 Msa0563560:three_prime_UTR 40.0%
CATAAAACAGACAAACAGCC+AGG - chr4_1:63936604-63936623 None:intergenic 40.0%
! CTGGCTGTTTGTCTGTTTTA+TGG + chr4_1:63936602-63936621 Msa0563560:three_prime_UTR 40.0%
GGAACACGTGTATAAGACGA+AGG + chr4_1:63935128-63935147 Msa0563560:CDS 45.0%
AGTTACCCTATGCGACCTTA+TGG + chr4_1:63935180-63935199 Msa0563560:CDS 45.0%
GTTACCCTATGCGACCTTAT+GGG + chr4_1:63935181-63935200 Msa0563560:CDS 45.0%
AGAACCCATAAGGTCGCATA+GGG - chr4_1:63935188-63935207 None:intergenic 45.0%
! CCATAACTCTCAGCTTGCTT+GGG + chr4_1:63935221-63935240 Msa0563560:CDS 45.0%
! GCTCAGATTAGTCAAGTTGC+TGG + chr4_1:63935246-63935265 Msa0563560:CDS 45.0%
! TTAGTCAAGTTGCTGGTCGT+AGG + chr4_1:63935253-63935272 Msa0563560:intron 45.0%
! TAGTCAAGTTGCTGGTCGTA+GGG + chr4_1:63935254-63935273 Msa0563560:intron 45.0%
!! GTTGGTAAGAAGCACTGACA+TGG + chr4_1:63935378-63935397 Msa0563560:intron 45.0%
! GTAACTAGATGCGTTGGTGT+CGG + chr4_1:63935473-63935492 Msa0563560:intron 45.0%
GCTACATTGCTAGAAGGGTT+TGG + chr4_1:63935529-63935548 Msa0563560:intron 45.0%
! CATTGCTAGAAGGGTTTGGT+TGG + chr4_1:63935533-63935552 Msa0563560:intron 45.0%
! TAGAAGGGTTTGGTTGGGAT+GGG + chr4_1:63935539-63935558 Msa0563560:intron 45.0%
!! AGGGGGAAATGTTGGTAGTA+GGG + chr4_1:63935700-63935719 Msa0563560:intron 45.0%
TTTCTCATAAAGGTCCCCGA+AGG - chr4_1:63935789-63935808 None:intergenic 45.0%
! GTGTTGTCAATTGCGGATTG+TGG + chr4_1:63935841-63935860 Msa0563560:intron 45.0%
! TTGCGGATTGTGGGTAATAG+TGG + chr4_1:63935851-63935870 Msa0563560:intron 45.0%
! AAACAGCCAGGAAGCACAAT+TGG - chr4_1:63936592-63936611 None:intergenic 45.0%
GGAACACGTGTATAAGACGA+AGG + chr4_1:63935128-63935147 Msa0563560:CDS 45.0%
AGTTACCCTATGCGACCTTA+TGG + chr4_1:63935180-63935199 Msa0563560:CDS 45.0%
GTTACCCTATGCGACCTTAT+GGG + chr4_1:63935181-63935200 Msa0563560:CDS 45.0%
AGAACCCATAAGGTCGCATA+GGG - chr4_1:63935188-63935207 None:intergenic 45.0%
! CCATAACTCTCAGCTTGCTT+GGG + chr4_1:63935221-63935240 Msa0563560:CDS 45.0%
! GCTCAGATTAGTCAAGTTGC+TGG + chr4_1:63935246-63935265 Msa0563560:CDS 45.0%
! TTAGTCAAGTTGCTGGTCGT+AGG + chr4_1:63935253-63935272 Msa0563560:intron 45.0%
! TAGTCAAGTTGCTGGTCGTA+GGG + chr4_1:63935254-63935273 Msa0563560:intron 45.0%
!! GTTGGTAAGAAGCACTGACA+TGG + chr4_1:63935378-63935397 Msa0563560:intron 45.0%
! GTAACTAGATGCGTTGGTGT+CGG + chr4_1:63935473-63935492 Msa0563560:intron 45.0%
GCTACATTGCTAGAAGGGTT+TGG + chr4_1:63935529-63935548 Msa0563560:intron 45.0%
! CATTGCTAGAAGGGTTTGGT+TGG + chr4_1:63935533-63935552 Msa0563560:intron 45.0%
! TAGAAGGGTTTGGTTGGGAT+GGG + chr4_1:63935539-63935558 Msa0563560:intron 45.0%
!! AGGGGGAAATGTTGGTAGTA+GGG + chr4_1:63935700-63935719 Msa0563560:intron 45.0%
TTTCTCATAAAGGTCCCCGA+AGG - chr4_1:63935789-63935808 None:intergenic 45.0%
! GTGTTGTCAATTGCGGATTG+TGG + chr4_1:63935841-63935860 Msa0563560:intron 45.0%
! TTGCGGATTGTGGGTAATAG+TGG + chr4_1:63935851-63935870 Msa0563560:intron 45.0%
! AAACAGCCAGGAAGCACAAT+TGG - chr4_1:63936592-63936611 None:intergenic 45.0%
!!! ATAAACATTTTTTTTTTTAT+AGG + chr4_1:63935642-63935661 Msa0563560:intron 5.0%
!!! ATAAACATTTTTTTTTTTAT+AGG + chr4_1:63935642-63935661 Msa0563560:intron 5.0%
CAGAACCCATAAGGTCGCAT+AGG - chr4_1:63935189-63935208 None:intergenic 50.0%
CTATGCGACCTTATGGGTTC+TGG + chr4_1:63935187-63935206 Msa0563560:CDS 50.0%
! GCCATAACTCTCAGCTTGCT+TGG + chr4_1:63935220-63935239 Msa0563560:CDS 50.0%
CCCAAGCAAGCTGAGAGTTA+TGG - chr4_1:63935224-63935243 None:intergenic 50.0%
CTCATTTGGACGATGAGGCA+GGG - chr4_1:63935308-63935327 None:intergenic 50.0%
!! TCCGGTGCTACATTGCTAGA+AGG + chr4_1:63935523-63935542 Msa0563560:intron 50.0%
CCCTTCTAGCAATGTAGCAC+CGG - chr4_1:63935527-63935546 None:intergenic 50.0%
!! CCGGTGCTACATTGCTAGAA+GGG + chr4_1:63935524-63935543 Msa0563560:intron 50.0%
! CTAGAAGGGTTTGGTTGGGA+TGG + chr4_1:63935538-63935557 Msa0563560:intron 50.0%
GTTTGGTTGGGATGGGATTG+TGG + chr4_1:63935546-63935565 Msa0563560:intron 50.0%
TTGGGATGGGATTGTGGACT+TGG + chr4_1:63935552-63935571 Msa0563560:intron 50.0%
TTAGAGGGAGGGGGAAATGT+TGG + chr4_1:63935692-63935711 Msa0563560:intron 50.0%
!! GAGGGGGAAATGTTGGTAGT+AGG + chr4_1:63935699-63935718 Msa0563560:intron 50.0%
!! GGGGGAAATGTTGGTAGTAG+GGG + chr4_1:63935701-63935720 Msa0563560:intron 50.0%
TGGTAGTAGGGGTTGAACCT+TGG + chr4_1:63935712-63935731 Msa0563560:intron 50.0%
AAACCACCGAGCTACTCCTT+CGG + chr4_1:63935770-63935789 Msa0563560:intron 50.0%
GCTACAGCACTGTTGCATTG+CGG - chr4_1:63935888-63935907 None:intergenic 50.0%
TAGTGGCCAATTGTGCTTCC+TGG + chr4_1:63936583-63936602 Msa0563560:three_prime_UTR 50.0%
CAGAACCCATAAGGTCGCAT+AGG - chr4_1:63935189-63935208 None:intergenic 50.0%
CTATGCGACCTTATGGGTTC+TGG + chr4_1:63935187-63935206 Msa0563560:CDS 50.0%
! GCCATAACTCTCAGCTTGCT+TGG + chr4_1:63935220-63935239 Msa0563560:CDS 50.0%
CCCAAGCAAGCTGAGAGTTA+TGG - chr4_1:63935224-63935243 None:intergenic 50.0%
CTCATTTGGACGATGAGGCA+GGG - chr4_1:63935308-63935327 None:intergenic 50.0%
!! TCCGGTGCTACATTGCTAGA+AGG + chr4_1:63935523-63935542 Msa0563560:intron 50.0%
CCCTTCTAGCAATGTAGCAC+CGG - chr4_1:63935527-63935546 None:intergenic 50.0%
!! CCGGTGCTACATTGCTAGAA+GGG + chr4_1:63935524-63935543 Msa0563560:intron 50.0%
! CTAGAAGGGTTTGGTTGGGA+TGG + chr4_1:63935538-63935557 Msa0563560:intron 50.0%
GTTTGGTTGGGATGGGATTG+TGG + chr4_1:63935546-63935565 Msa0563560:intron 50.0%
TTGGGATGGGATTGTGGACT+TGG + chr4_1:63935552-63935571 Msa0563560:intron 50.0%
TTAGAGGGAGGGGGAAATGT+TGG + chr4_1:63935692-63935711 Msa0563560:intron 50.0%
!! GAGGGGGAAATGTTGGTAGT+AGG + chr4_1:63935699-63935718 Msa0563560:intron 50.0%
!! GGGGGAAATGTTGGTAGTAG+GGG + chr4_1:63935701-63935720 Msa0563560:intron 50.0%
TGGTAGTAGGGGTTGAACCT+TGG + chr4_1:63935712-63935731 Msa0563560:intron 50.0%
AAACCACCGAGCTACTCCTT+CGG + chr4_1:63935770-63935789 Msa0563560:intron 50.0%
GCTACAGCACTGTTGCATTG+CGG - chr4_1:63935888-63935907 None:intergenic 50.0%
TAGTGGCCAATTGTGCTTCC+TGG + chr4_1:63936583-63936602 Msa0563560:three_prime_UTR 50.0%
CCTCATTTGGACGATGAGGC+AGG - chr4_1:63935309-63935328 None:intergenic 55.0%
CCTGCCTCATCGTCCAAATG+AGG + chr4_1:63935306-63935325 Msa0563560:intron 55.0%
!! GATGCGTTGGTGTCGGACAT+TGG + chr4_1:63935480-63935499 Msa0563560:intron 55.0%
!! GGTTTGGCTTGGGACACCAA+AGG - chr4_1:63935755-63935774 None:intergenic 55.0%
! GTAGCTCGGTGGTTTGGCTT+GGG - chr4_1:63935765-63935784 None:intergenic 55.0%
! AGTAGCTCGGTGGTTTGGCT+TGG - chr4_1:63935766-63935785 None:intergenic 55.0%
! GAAGGAGTAGCTCGGTGGTT+TGG - chr4_1:63935771-63935790 None:intergenic 55.0%
AACCACCGAGCTACTCCTTC+GGG + chr4_1:63935771-63935790 Msa0563560:intron 55.0%
CCTCATTTGGACGATGAGGC+AGG - chr4_1:63935309-63935328 None:intergenic 55.0%
CCTGCCTCATCGTCCAAATG+AGG + chr4_1:63935306-63935325 Msa0563560:intron 55.0%
!! GATGCGTTGGTGTCGGACAT+TGG + chr4_1:63935480-63935499 Msa0563560:intron 55.0%
!! GGTTTGGCTTGGGACACCAA+AGG - chr4_1:63935755-63935774 None:intergenic 55.0%
! GTAGCTCGGTGGTTTGGCTT+GGG - chr4_1:63935765-63935784 None:intergenic 55.0%
! AGTAGCTCGGTGGTTTGGCT+TGG - chr4_1:63935766-63935785 None:intergenic 55.0%
! GAAGGAGTAGCTCGGTGGTT+TGG - chr4_1:63935771-63935790 None:intergenic 55.0%
AACCACCGAGCTACTCCTTC+GGG + chr4_1:63935771-63935790 Msa0563560:intron 55.0%
GGATAGGTGCGCGAAGACGA+AGG + chr4_1:63935149-63935168 Msa0563560:CDS 60.0%
GCGCGAAGACGAAGGAGATG+TGG + chr4_1:63935157-63935176 Msa0563560:CDS 60.0%
ACCACCGAGCTACTCCTTCG+GGG + chr4_1:63935772-63935791 Msa0563560:intron 60.0%
AGGTCCCCGAAGGAGTAGCT+CGG - chr4_1:63935779-63935798 None:intergenic 60.0%
GGATAGGTGCGCGAAGACGA+AGG + chr4_1:63935149-63935168 Msa0563560:CDS 60.0%
GCGCGAAGACGAAGGAGATG+TGG + chr4_1:63935157-63935176 Msa0563560:CDS 60.0%
ACCACCGAGCTACTCCTTCG+GGG + chr4_1:63935772-63935791 Msa0563560:intron 60.0%
AGGTCCCCGAAGGAGTAGCT+CGG - chr4_1:63935779-63935798 None:intergenic 60.0%
TCCCCGAAGGAGTAGCTCGG+TGG - chr4_1:63935776-63935795 None:intergenic 65.0%
TCCCCGAAGGAGTAGCTCGG+TGG - chr4_1:63935776-63935795 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 63935048 63936729 63935048 ID=Msa0563560;Name=Msa0563560
chr4_1 mRNA 63935048 63936729 63935048 ID=Msa0563560-mRNA-1;Parent=Msa0563560;Name=Msa0563560-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0.5|1|1|1|2|344|80
chr4_1 exon 63935048 63935270 63935048 ID=Msa0563560-mRNA-1:exon:20372;Parent=Msa0563560-mRNA-1
chr4_1 exon 63936366 63936729 63936366 ID=Msa0563560-mRNA-1:exon:20373;Parent=Msa0563560-mRNA-1
chr4_1 CDS 63935048 63935270 63935048 ID=Msa0563560-mRNA-1:cds;Parent=Msa0563560-mRNA-1
chr4_1 CDS 63936366 63936385 63936366 ID=Msa0563560-mRNA-1:cds;Parent=Msa0563560-mRNA-1
chr4_1 three_prime_UTR 63936386 63936729 63936386 ID=Msa0563560-mRNA-1:three_prime_utr;Parent=Msa0563560-mRNA-1
Gene Sequence

>Msa0563560

ATGGCTTTCATAATGGAATTCGCTGAGAATCTGGTTCTGAAATTGATGGAAGATCCAAATGAAAGGGATCGTAGATTCAGGGAACACGTGTATAAGACGAAGGATAGGTGCGCGAAGACGAAGGAGATGTGGAGTTACCCTATGCGACCTTATGGGTTCTGGACATTTGAACGCCATAACTCTCAGCTTGCTTGGGATGCTCAGATTAGTCAAGTTGCTGGTCTGATGCCGAAGTTGTCTTAA
Protein sequence

>Msa0563560

MAFIMEFAENLVLKLMEDPNERDRRFREHVYKTKDRCAKTKEMWSYPMRPYGFWTFERHNSQLAWDAQISQVAGLMPKLS*