AlfalfaGEDB | Msa0881460

M. sativa cultivar zhongmu-4 / Msa0881460

KEGG: K01802
GO: GO:0000413 GO:0003674 GO:0003755 GO:0003824 GO:0005488 GO:0005527 GO:0005528 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006457 GO:0006464 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016859 GO:0018193 GO:0018208 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0061077 GO:0071704 GO:0097159 GO:0140096 GO:1901363 GO:1901564
PFAM: PF00254.31

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0881460	XP_003593250.1	98.214	112	2	0	1	112	1	112	1.21e-74	226

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0881460	sp\|O04287\|FKB12_VICFA	88.393	112	13	0	1	112	1	112	2.44e-71	211

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0881460	B7FFD7	98.214	112	2	0	1	112	1	112	5.78e-75	226

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa0881460	0.838947	2.124365e-57	-8.615850e-47
Msa0003700	Msa0881460	0.806127	9.566527e-50	-8.615850e-47
Msa0017510	Msa0881460	0.801578	8.428393e-49	-8.615850e-47
Msa0044000	Msa0881460	0.804796	1.819020e-49	-8.615850e-47
Msa0056470	Msa0881460	0.817956	2.520256e-52	-8.615850e-47
Msa0064350	Msa0881460	0.826220	3.056980e-54	-8.615850e-47
Msa0064380	Msa0881460	0.802646	5.083523e-49	-8.615850e-47
Msa0075170	Msa0881460	0.808194	3.490992e-50	-8.615850e-47
Msa0090360	Msa0881460	0.803062	4.169273e-49	-8.615850e-47
Msa0602870	Msa0881460	0.852149	5.580466e-61	-8.615850e-47
Msa0607050	Msa0881460	0.819891	9.153305e-53	-8.615850e-47
Msa0608320	Msa0881460	0.811211	7.841227e-51	-8.615850e-47
Msa0615710	Msa0881460	0.848132	7.446149e-60	-8.615850e-47
Msa0643890	Msa0881460	0.848310	6.650738e-60	-8.615850e-47
Msa0661300	Msa0881460	0.805466	1.317088e-49	-8.615850e-47
Msa0683730	Msa0881460	0.805472	1.313250e-49	-8.615850e-47
Msa0690150	Msa0881460	0.817895	2.601332e-52	-8.615850e-47
Msa0692980	Msa0881460	0.828002	1.144962e-54	-8.615850e-47
Msa0702470	Msa0881460	0.802330	5.905213e-49	-8.615850e-47
Msa0709400	Msa0881460	0.828710	7.727374e-55	-8.615850e-47
Msa0716690	Msa0881460	0.807578	4.719706e-50	-8.615850e-47
Msa0729720	Msa0881460	0.807594	4.683694e-50	-8.615850e-47
Msa0801110	Msa0881460	0.800510	1.393423e-48	-8.615850e-47
Msa0123560	Msa0881460	0.803045	4.204423e-49	-8.615850e-47
Msa0127800	Msa0881460	0.809099	2.236525e-50	-8.615850e-47
Msa0140820	Msa0881460	0.843843	1.092047e-58	-8.615850e-47
Msa0144300	Msa0881460	0.811218	7.814948e-51	-8.615850e-47
Msa0157290	Msa0881460	0.800770	1.233177e-48	-8.615850e-47
Msa0205510	Msa0881460	0.810331	1.215629e-50	-8.615850e-47
Msa0226540	Msa0881460	0.801369	9.303874e-49	-8.615850e-47
Msa0229230	Msa0881460	0.800171	1.633505e-48	-8.615850e-47
Msa0231980	Msa0881460	0.810787	9.686850e-51	-8.615850e-47
Msa0232050	Msa0881460	0.815949	7.112878e-52	-8.615850e-47
Msa0366890	Msa0881460	0.810942	8.970064e-51	-8.615850e-47
Msa0376310	Msa0881460	0.806390	8.419851e-50	-8.615850e-47
Msa0391690	Msa0881460	0.810736	9.936278e-51	-8.615850e-47
Msa0402790	Msa0881460	0.800287	1.547192e-48	-8.615850e-47
Msa0436050	Msa0881460	0.824645	7.216556e-54	-8.615850e-47
Msa0468330	Msa0881460	0.822371	2.454918e-53	-8.615850e-47
Msa0469990	Msa0881460	0.837383	5.370197e-57	-8.615850e-47
Msa0243090	Msa0881460	0.811435	7.010148e-51	-8.615850e-47
Msa0298700	Msa0881460	0.809731	1.636582e-50	-8.615850e-47
Msa0315170	Msa0881460	0.807943	3.948523e-50	-8.615850e-47
Msa0326480	Msa0881460	0.801050	1.081088e-48	-8.615850e-47
Msa0334360	Msa0881460	0.814278	1.671585e-51	-8.615850e-47
Msa0334550	Msa0881460	0.802107	6.564435e-49	-8.615850e-47
Msa0341050	Msa0881460	0.822680	2.080582e-53	-8.615850e-47
Msa0346430	Msa0881460	0.811870	5.639425e-51	-8.615850e-47
Msa0485340	Msa0881460	0.812860	3.427129e-51	-8.615850e-47
Msa0521050	Msa0881460	0.808973	2.379802e-50	-8.615850e-47
Msa0524500	Msa0881460	0.820297	7.389582e-53	-8.615850e-47
Msa0547890	Msa0881460	0.801039	1.086742e-48	-8.615850e-47
Msa0556060	Msa0881460	0.820599	6.298920e-53	-8.615850e-47
Msa0563560	Msa0881460	0.833993	3.876247e-56	-8.615850e-47
Msa0847770	Msa0881460	0.806408	8.345869e-50	-8.615850e-47
Msa0881460	Msa0924470	0.808079	3.694585e-50	-8.615850e-47
Msa0881460	Msa0963270	0.850104	2.107368e-60	-8.615850e-47
Msa0881460	Msa0992020	0.812059	5.128077e-51	-8.615850e-47
Msa0881460	Msa0993290	0.800653	1.303140e-48	-8.615850e-47
Msa0881460	Msa0993730	0.803568	3.276818e-49	-8.615850e-47
Msa0881460	Msa1013400	0.803839	2.878283e-49	-8.615850e-47
Msa0881460	Msa1040400	0.805651	1.204150e-49	-8.615850e-47
Msa0881460	Msa1046020	0.804401	2.198546e-49	-8.615850e-47
Msa0881460	Msa1048160	0.802478	5.504333e-49	-8.615850e-47
Msa0881460	Msa1078170	0.807306	5.390389e-50	-8.615850e-47
Msa0881460	Msa1081930	0.818019	2.439013e-52	-8.615850e-47
Msa0881460	Msa1085800	0.800778	1.228745e-48	-8.615850e-47
Msa0881460	Msa1124960	0.823869	1.098113e-53	-8.615850e-47
Msa0881460	Msa1134110	0.805240	1.468618e-49	-8.615850e-47
Msa0881460	Msa1165940	0.821742	3.435446e-53	-8.615850e-47
Msa0881460	Msa1189070	0.816997	4.144211e-52	-8.615850e-47
Msa0881460	Msa1225700	0.806789	6.934869e-50	-8.615850e-47
Msa0881460	Msa1239410	0.822400	2.417184e-53	-8.615850e-47
Msa0881460	Msa1239910	0.821469	3.970579e-53	-8.615850e-47
Msa0881460	Msa1244600	0.828812	7.302592e-55	-8.615850e-47
Msa0881460	Msa1250530	0.819551	1.094301e-52	-8.615850e-47
Msa0881460	Msa1251160	0.815492	8.990572e-52	-8.615850e-47
Msa0881460	Msa1251730	0.803605	3.218345e-49	-8.615850e-47
Msa0881460	Msa1253020	0.810631	1.046892e-50	-8.615850e-47
Msa0881460	Msa1254730	0.800517	1.388516e-48	-8.615850e-47
Msa0881460	Msa1279400	0.831365	1.739480e-55	-8.615850e-47
Msa0881460	Msa1284550	0.817378	3.401554e-52	-8.615850e-47
Msa0881460	Msa1293510	0.803704	3.069483e-49	-8.615850e-47
Msa0881460	Msa1356140	0.803550	3.304257e-49	-8.615850e-47
Msa0881460	Msa1360440	0.803809	2.919297e-49	-8.615850e-47
Msa0881460	Msa1367590	0.818377	2.022998e-52	-8.615850e-47
Msa0881460	Msa1375560	0.802802	4.719328e-49	-8.615850e-47
Msa0881460	Msa1382540	0.805685	1.184915e-49	-8.615850e-47
Msa0881460	Msa1451160	0.809913	1.495940e-50	-8.615850e-47
Msa0881460	Msa1451550	0.812578	3.951627e-51	-8.615850e-47
Msa0881460	Msa1451560	0.803168	3.964706e-49	-8.615850e-47
Msa0881460	Msa1462290	0.818048	2.402516e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0881460	MtrunA17_Chr2g0279511	98.214	112	2	0	1	112	1	112	1.11e-78	226
Msa0881460	MtrunA17_Chr8g0377771	45.455	110	54	4	2	110	38	142	8.86e-20	83.2
Msa0881460	MtrunA17_Chr8g0377771	36.036	111	65	3	3	110	272	379	6.39e-14	66.2
Msa0881460	MtrunA17_Chr5g0435521	40.000	110	63	3	2	110	12	119	1.16e-19	79.3
Msa0881460	MtrunA17_Chr8g0352971	42.308	104	49	3	11	110	9	105	1.66e-17	76.3
Msa0881460	MtrunA17_Chr7g0234801	45.263	95	47	3	16	110	49	138	2.89e-17	75.9
Msa0881460	MtrunA17_Chr8g0339141	46.316	95	46	3	16	110	55	144	4.92e-17	75.1
Msa0881460	MtrunA17_Chr7g0255551	44.037	109	55	4	2	109	21	124	2.07e-16	73.6
Msa0881460	MtrunA17_Chr4g0052911	36.364	110	56	4	11	110	133	238	2.98e-12	60.8
Msa0881460	MtrunA17_Chr4g0014351	35.644	101	58	3	12	111	774	868	5.15e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0881460	AT5G64350.1	86.607	112	15	0	1	112	1	112	3.53e-71	208
Msa0881460	AT3G55520.2	35.780	109	69	1	2	110	12	119	1.85e-17	73.9
Msa0881460	AT3G55520.3	35.780	109	69	1	2	110	12	119	1.85e-17	73.9
Msa0881460	AT3G55520.1	35.780	109	69	1	2	110	12	119	1.85e-17	73.9
Msa0881460	AT5G48570.1	40.000	110	60	4	2	110	47	151	9.73e-16	72.0
Msa0881460	AT3G25230.2	40.367	109	59	4	2	109	39	142	4.68e-15	70.1
Msa0881460	AT3G25230.1	40.367	109	59	4	2	109	39	142	6.01e-15	69.7
Msa0881460	AT4G25340.1	42.157	102	52	4	12	112	382	477	1.62e-14	68.6
Msa0881460	AT5G05420.1	41.176	102	52	4	12	112	49	143	2.40e-13	62.4
Msa0881460	AT5G45680.1	40.367	109	51	4	11	109	101	205	4.62e-13	62.8

Find 50 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCTCCAATATTAATATT+TGG	0.175040	6_1:+28655380	Msa0881460:three_prime_UTR
ATTTGTTGATCAATATTATT+TGG	0.184739	6_1:+28655439	Msa0881460:three_prime_UTR
CGCTAGTGGCTTTCCTGCTT+GGG	0.208113	6_1:+28655147	Msa0881460:CDS
GAAATAAAAGAAAGATCTTT+AGG	0.263241	6_1:-28655525	None:intergenic
GTGACCTTTCTCAGAAATTC+TGG	0.269473	6_1:+28653988	Msa0881460:CDS
TACCAAAGGACAAACTAAAA+AGG	0.281726	6_1:-28655229	None:intergenic
TGCTCTCCTGATTATGCTTA+TGG	0.301017	6_1:+28655124	Msa0881460:CDS
CAACAAATTGATAAAGAAAC+AGG	0.328558	6_1:-28655424	None:intergenic
TCGGAGAAGTTGCTCGTCTT+CGG	0.329775	6_1:+28654538	Msa0881460:CDS
AATACAACCCAACTCTGTTT+TGG	0.332199	6_1:+28655171	Msa0881460:CDS
GCGCTAGTGGCTTTCCTGCT+TGG	0.337892	6_1:+28655146	Msa0881460:CDS
CGGAGAAGTTGCTCGTCTTC+GGG	0.345948	6_1:+28654539	Msa0881460:CDS
ACACTACCAAATATTAATAT+TGG	0.352985	6_1:-28655386	None:intergenic
AATCCATTCACTTTCAAAAT+CGG	0.376739	6_1:+28654118	Msa0881460:CDS
GAAAGAAGGAAGAAGAAAGA+TGG	0.415250	6_1:+28653710	Msa0881460:exon
AAAGAAGGAAGAAGAAAGAT+GGG	0.430314	6_1:+28653711	Msa0881460:exon
GTTGGCTTAGGACCGTTTCC+AGG	0.437240	6_1:-28653756	None:intergenic
TATTTGGCAATGCATGGTCT+TGG	0.437640	6_1:+28655455	Msa0881460:three_prime_UTR
GAGAGAGTGCTCACCGTATC+CGG	0.441515	6_1:-28653808	None:intergenic
CTAGCGCCATAAGCATAATC+AGG	0.449393	6_1:-28655130	None:intergenic
TCAAACTCCAAAACAGAGTT+GGG	0.461799	6_1:-28655178	None:intergenic
CTCAAACTCCAAAACAGAGT+TGG	0.473985	6_1:-28655179	None:intergenic
GGTGTCATTGGCATGCAAAT+CGG	0.478078	6_1:+28654519	Msa0881460:CDS
AAAGTGAATGGATTCTGGCC+AGG	0.495829	6_1:-28654109	None:intergenic
GCAGATATCACTCAAAGTGC+AGG	0.504337	6_1:-28655307	None:intergenic
CAAATCGTTCGACCTGGAAA+CGG	0.515678	6_1:+28653744	Msa0881460:CDS
ACCTCCAGAATTTCTGAGAA+AGG	0.516160	6_1:-28653992	None:intergenic
ACTGTCCACTGCACCGGATA+CGG	0.518197	6_1:+28653795	Msa0881460:CDS
TTCACTTTCAAAATCGGCAA+AGG	0.528436	6_1:+28654124	Msa0881460:CDS
GGATGGGATGAAGGTGTCAT+TGG	0.529844	6_1:+28654507	Msa0881460:intron
CAATATTATTTGGCAATGCA+TGG	0.531070	6_1:+28655449	Msa0881460:three_prime_UTR
GCATAAGATCGAGTGAAAGA+AGG	0.537335	6_1:+28653696	Msa0881460:five_prime_UTR
ATACATGTCCACAGAGATGT+AGG	0.540488	6_1:-28655275	None:intergenic
TATTACAGTACAAAAGATCC+TGG	0.543772	6_1:+28654091	Msa0881460:intron
ATTGGAGAGTTTGAGATCAG+TGG	0.545467	6_1:-28655368	None:intergenic
GATTATGCTTATGGCGCTAG+TGG	0.545830	6_1:+28655133	Msa0881460:CDS
AACGGTCCTAAGCCAACTCC+TGG	0.550713	6_1:+28653762	Msa0881460:CDS
AACGTCACTGTCCACTGCAC+CGG	0.551030	6_1:+28653789	Msa0881460:CDS
TTGTGTGTAGGATGGGATGA+AGG	0.556041	6_1:+28654498	Msa0881460:intron
TTGGGTTGTATTCCCCAAGC+AGG	0.558631	6_1:-28655160	None:intergenic
CAGATATCACTCAAAGTGCA+GGG	0.569024	6_1:-28655306	None:intergenic
GCTAGTGGCTTTCCTGCTTG+GGG	0.575669	6_1:+28655148	Msa0881460:CDS
ACCTTTCTCAGAAATTCTGG+AGG	0.582702	6_1:+28653991	Msa0881460:CDS
GGCAAAGGTTCTGTCATCAA+AGG	0.586910	6_1:+28654139	Msa0881460:CDS
GCTCACCGTATCCGGTGCAG+TGG	0.596990	6_1:-28653800	None:intergenic
GAGAAGCAAATCGTTCGACC+TGG	0.621651	6_1:+28653738	Msa0881460:CDS
TCATAATAATAATTTACCAA+AGG	0.632106	6_1:-28655243	None:intergenic
TTGAGTATCCTACATCTCTG+TGG	0.668816	6_1:+28655267	Msa0881460:three_prime_UTR
AACAAATTGATAAAGAAACA+GGG	0.676496	6_1:-28655423	None:intergenic
AGATATCACTCAAAGTGCAG+GGG	0.704961	6_1:-28655305	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATATTAAAAACAAAAAA+TGG	-	chr6_1:28654049-28654068	None:intergenic	10.0%
!!	ACAATATTAAAAACAAAAAA+TGG	-	chr6_1:28654049-28654068	None:intergenic	10.0%
!!	TAAAATGAAAGATAAGAAAT+GGG	-	chr6_1:28653854-28653873	None:intergenic	15.0%
!!	TTAAAATGAAAGATAAGAAA+TGG	-	chr6_1:28653855-28653874	None:intergenic	15.0%
!!	ATATAAGCTAAAATAGTTTA+TGG	-	chr6_1:28654706-28654725	None:intergenic	15.0%
!!!	TATGATAAGTGTTTATTTTA+TGG	+	chr6_1:28654827-28654846	Msa0881460:intron	15.0%
!!	TCATAATAATAATTTACCAA+AGG	-	chr6_1:28655246-28655265	None:intergenic	15.0%
!!!	ATTTGTTGATCAATATTATT+TGG	+	chr6_1:28655439-28655458	Msa0881460:three_prime_UTR	15.0%
!!	TAAAATGAAAGATAAGAAAT+GGG	-	chr6_1:28653854-28653873	None:intergenic	15.0%
!!	TTAAAATGAAAGATAAGAAA+TGG	-	chr6_1:28653855-28653874	None:intergenic	15.0%
!!	ATATAAGCTAAAATAGTTTA+TGG	-	chr6_1:28654706-28654725	None:intergenic	15.0%
!!!	TATGATAAGTGTTTATTTTA+TGG	+	chr6_1:28654827-28654846	Msa0881460:intron	15.0%
!!	TCATAATAATAATTTACCAA+AGG	-	chr6_1:28655246-28655265	None:intergenic	15.0%
!!!	ATTTGTTGATCAATATTATT+TGG	+	chr6_1:28655439-28655458	Msa0881460:three_prime_UTR	15.0%
!!	AAAATGAAAGATAAGAAATG+GGG	-	chr6_1:28653853-28653872	None:intergenic	20.0%
!!!	TACTACTTTTACTTTATTTC+TGG	+	chr6_1:28654393-28654412	Msa0881460:intron	20.0%
!!	GTTGATACTTTGTTAAATAA+TGG	+	chr6_1:28655055-28655074	Msa0881460:intron	20.0%
!!	AACTCTCCAATATTAATATT+TGG	+	chr6_1:28655380-28655399	Msa0881460:three_prime_UTR	20.0%
!!	ACACTACCAAATATTAATAT+TGG	-	chr6_1:28655389-28655408	None:intergenic	20.0%
!!	AACAAATTGATAAAGAAACA+GGG	-	chr6_1:28655426-28655445	None:intergenic	20.0%
!!	GAAATAAAAGAAAGATCTTT+AGG	-	chr6_1:28655528-28655547	None:intergenic	20.0%
!!	AAAATGAAAGATAAGAAATG+GGG	-	chr6_1:28653853-28653872	None:intergenic	20.0%
!!!	TACTACTTTTACTTTATTTC+TGG	+	chr6_1:28654393-28654412	Msa0881460:intron	20.0%
!!	GTTGATACTTTGTTAAATAA+TGG	+	chr6_1:28655055-28655074	Msa0881460:intron	20.0%
!!	AACTCTCCAATATTAATATT+TGG	+	chr6_1:28655380-28655399	Msa0881460:three_prime_UTR	20.0%
!!	ACACTACCAAATATTAATAT+TGG	-	chr6_1:28655389-28655408	None:intergenic	20.0%
!!	AACAAATTGATAAAGAAACA+GGG	-	chr6_1:28655426-28655445	None:intergenic	20.0%
!!	GAAATAAAAGAAAGATCTTT+AGG	-	chr6_1:28655528-28655547	None:intergenic	20.0%
!!!	AAATGGGGTTTTTAGTTTTA+GGG	-	chr6_1:28653838-28653857	None:intergenic	25.0%
!	AACAAAAAATGGAATGACAA+GGG	-	chr6_1:28654038-28654057	None:intergenic	25.0%
!	AAACAAAAAATGGAATGACA+AGG	-	chr6_1:28654039-28654058	None:intergenic	25.0%
!	AATCCATTCACTTTCAAAAT+CGG	+	chr6_1:28654118-28654137	Msa0881460:CDS	25.0%
!!!	TTTATAACTTGTTACCACTT+TGG	+	chr6_1:28654589-28654608	Msa0881460:intron	25.0%
!!!	TTATAACTTGTTACCACTTT+GGG	+	chr6_1:28654590-28654609	Msa0881460:intron	25.0%
!	AATGGGAATTCAACATATTA+AGG	-	chr6_1:28654889-28654908	None:intergenic	25.0%
!	CAACAAATTGATAAAGAAAC+AGG	-	chr6_1:28655427-28655446	None:intergenic	25.0%
!!!	TTCAGTTTATATCGTTTTTG+TGG	+	chr6_1:28655500-28655519	Msa0881460:three_prime_UTR	25.0%
!!!	AAATGGGGTTTTTAGTTTTA+GGG	-	chr6_1:28653838-28653857	None:intergenic	25.0%
!	AACAAAAAATGGAATGACAA+GGG	-	chr6_1:28654038-28654057	None:intergenic	25.0%
!	AAACAAAAAATGGAATGACA+AGG	-	chr6_1:28654039-28654058	None:intergenic	25.0%
!	AATCCATTCACTTTCAAAAT+CGG	+	chr6_1:28654118-28654137	Msa0881460:CDS	25.0%
!!!	TTTATAACTTGTTACCACTT+TGG	+	chr6_1:28654589-28654608	Msa0881460:intron	25.0%
!!!	TTATAACTTGTTACCACTTT+GGG	+	chr6_1:28654590-28654609	Msa0881460:intron	25.0%
!	AATGGGAATTCAACATATTA+AGG	-	chr6_1:28654889-28654908	None:intergenic	25.0%
!	CAACAAATTGATAAAGAAAC+AGG	-	chr6_1:28655427-28655446	None:intergenic	25.0%
!!!	TTCAGTTTATATCGTTTTTG+TGG	+	chr6_1:28655500-28655519	Msa0881460:three_prime_UTR	25.0%
	AAAGAAGGAAGAAGAAAGAT+GGG	+	chr6_1:28653711-28653730	Msa0881460:exon	30.0%
!!!	GAAATGGGGTTTTTAGTTTT+AGG	-	chr6_1:28653839-28653858	None:intergenic	30.0%
	TATTACAGTACAAAAGATCC+TGG	+	chr6_1:28654091-28654110	Msa0881460:intron	30.0%
!!	TTTTGAAAGTGAATGGATTC+TGG	-	chr6_1:28654117-28654136	None:intergenic	30.0%
!	GTGTAGAGTTTTTACACTAT+CGG	+	chr6_1:28654211-28654230	Msa0881460:intron	30.0%
	CAATGTATATTCAGATGTGT+TGG	+	chr6_1:28654439-28654458	Msa0881460:intron	30.0%
	CAACATATTAAGGTCATGTT+TGG	-	chr6_1:28654879-28654898	None:intergenic	30.0%
	GAGTAAAAACAAACAGCAAT+GGG	-	chr6_1:28654906-28654925	None:intergenic	30.0%
	TGAGTAAAAACAAACAGCAA+TGG	-	chr6_1:28654907-28654926	None:intergenic	30.0%
!!!	AACCTTTTTAGTTTGTCCTT+TGG	+	chr6_1:28655227-28655246	Msa0881460:three_prime_UTR	30.0%
	TACCAAAGGACAAACTAAAA+AGG	-	chr6_1:28655232-28655251	None:intergenic	30.0%
	CAATATTATTTGGCAATGCA+TGG	+	chr6_1:28655449-28655468	Msa0881460:three_prime_UTR	30.0%
	AAAGAAGGAAGAAGAAAGAT+GGG	+	chr6_1:28653711-28653730	Msa0881460:exon	30.0%
!!!	GAAATGGGGTTTTTAGTTTT+AGG	-	chr6_1:28653839-28653858	None:intergenic	30.0%
	TATTACAGTACAAAAGATCC+TGG	+	chr6_1:28654091-28654110	Msa0881460:intron	30.0%
!!	TTTTGAAAGTGAATGGATTC+TGG	-	chr6_1:28654117-28654136	None:intergenic	30.0%
!	GTGTAGAGTTTTTACACTAT+CGG	+	chr6_1:28654211-28654230	Msa0881460:intron	30.0%
	CAATGTATATTCAGATGTGT+TGG	+	chr6_1:28654439-28654458	Msa0881460:intron	30.0%
	CAACATATTAAGGTCATGTT+TGG	-	chr6_1:28654879-28654898	None:intergenic	30.0%
	GAGTAAAAACAAACAGCAAT+GGG	-	chr6_1:28654906-28654925	None:intergenic	30.0%
	TGAGTAAAAACAAACAGCAA+TGG	-	chr6_1:28654907-28654926	None:intergenic	30.0%
!!!	AACCTTTTTAGTTTGTCCTT+TGG	+	chr6_1:28655227-28655246	Msa0881460:three_prime_UTR	30.0%
	TACCAAAGGACAAACTAAAA+AGG	-	chr6_1:28655232-28655251	None:intergenic	30.0%
	CAATATTATTTGGCAATGCA+TGG	+	chr6_1:28655449-28655468	Msa0881460:three_prime_UTR	30.0%
	GAAAGAAGGAAGAAGAAAGA+TGG	+	chr6_1:28653710-28653729	Msa0881460:exon	35.0%
!!	TGATTGTGTTGATGATGATC+AGG	+	chr6_1:28653957-28653976	Msa0881460:intron	35.0%
!	TGATGATGATCAGGCAAAAA+CGG	+	chr6_1:28653966-28653985	Msa0881460:intron	35.0%
!!!	TTGCCGATTTTGAAAGTGAA+TGG	-	chr6_1:28654124-28654143	None:intergenic	35.0%
	TTCACTTTCAAAATCGGCAA+AGG	+	chr6_1:28654124-28654143	Msa0881460:CDS	35.0%
	TTACACACTTGTGAGACTTT+AGG	-	chr6_1:28654303-28654322	None:intergenic	35.0%
	CAATTCAATAAAGCCCAAAG+TGG	-	chr6_1:28654606-28654625	None:intergenic	35.0%
!!	CACTTTGGGCTTTATTGAAT+TGG	+	chr6_1:28654604-28654623	Msa0881460:intron	35.0%
!	TTTTTACTCACGCACATACA+AGG	+	chr6_1:28654917-28654936	Msa0881460:intron	35.0%
	TATCAACAAGGTCAATGACA+GGG	-	chr6_1:28655095-28655114	None:intergenic	35.0%
	TTATCAACAAGGTCAATGAC+AGG	-	chr6_1:28655096-28655115	None:intergenic	35.0%
!	AATACAACCCAACTCTGTTT+TGG	+	chr6_1:28655171-28655190	Msa0881460:CDS	35.0%
	TCAAACTCCAAAACAGAGTT+GGG	-	chr6_1:28655181-28655200	None:intergenic	35.0%
!!!	TGTTTTGGAGTTTGAGATTG+AGG	+	chr6_1:28655186-28655205	Msa0881460:CDS	35.0%
	GAAAGAAGGAAGAAGAAAGA+TGG	+	chr6_1:28653710-28653729	Msa0881460:exon	35.0%
!!	TGATTGTGTTGATGATGATC+AGG	+	chr6_1:28653957-28653976	Msa0881460:intron	35.0%
!	TGATGATGATCAGGCAAAAA+CGG	+	chr6_1:28653966-28653985	Msa0881460:intron	35.0%
!!!	TTGCCGATTTTGAAAGTGAA+TGG	-	chr6_1:28654124-28654143	None:intergenic	35.0%
	TTCACTTTCAAAATCGGCAA+AGG	+	chr6_1:28654124-28654143	Msa0881460:CDS	35.0%
	TTACACACTTGTGAGACTTT+AGG	-	chr6_1:28654303-28654322	None:intergenic	35.0%
	CAATTCAATAAAGCCCAAAG+TGG	-	chr6_1:28654606-28654625	None:intergenic	35.0%
!!	CACTTTGGGCTTTATTGAAT+TGG	+	chr6_1:28654604-28654623	Msa0881460:intron	35.0%
!	TTTTTACTCACGCACATACA+AGG	+	chr6_1:28654917-28654936	Msa0881460:intron	35.0%
	TATCAACAAGGTCAATGACA+GGG	-	chr6_1:28655095-28655114	None:intergenic	35.0%
	TTATCAACAAGGTCAATGAC+AGG	-	chr6_1:28655096-28655115	None:intergenic	35.0%
!	AATACAACCCAACTCTGTTT+TGG	+	chr6_1:28655171-28655190	Msa0881460:CDS	35.0%
	TCAAACTCCAAAACAGAGTT+GGG	-	chr6_1:28655181-28655200	None:intergenic	35.0%
!!!	TGTTTTGGAGTTTGAGATTG+AGG	+	chr6_1:28655186-28655205	Msa0881460:CDS	35.0%
	GCATAAGATCGAGTGAAAGA+AGG	+	chr6_1:28653696-28653715	Msa0881460:five_prime_UTR	40.0%
	GTGACCTTTCTCAGAAATTC+TGG	+	chr6_1:28653988-28654007	Msa0881460:CDS	40.0%
	ACCTTTCTCAGAAATTCTGG+AGG	+	chr6_1:28653991-28654010	Msa0881460:CDS	40.0%
!	ACCTCCAGAATTTCTGAGAA+AGG	-	chr6_1:28653995-28654014	None:intergenic	40.0%
!	TTCTCAGAAATTCTGGAGGT+TGG	+	chr6_1:28653995-28654014	Msa0881460:intron	40.0%
	TCCAACTATCTGTTGTGTGT+AGG	+	chr6_1:28654486-28654505	Msa0881460:intron	40.0%
	TCCTACACACAACAGATAGT+TGG	-	chr6_1:28654490-28654509	None:intergenic	40.0%
	ACTATCTGTTGTGTGTAGGA+TGG	+	chr6_1:28654490-28654509	Msa0881460:intron	40.0%
	CTATCTGTTGTGTGTAGGAT+GGG	+	chr6_1:28654491-28654510	Msa0881460:intron	40.0%
	GCACTGCAAGATTATCAACA+AGG	-	chr6_1:28655107-28655126	None:intergenic	40.0%
	TGCTCTCCTGATTATGCTTA+TGG	+	chr6_1:28655124-28655143	Msa0881460:CDS	40.0%
	CTCAAACTCCAAAACAGAGT+TGG	-	chr6_1:28655182-28655201	None:intergenic	40.0%
	TTGAGTATCCTACATCTCTG+TGG	+	chr6_1:28655267-28655286	Msa0881460:three_prime_UTR	40.0%
	ATACATGTCCACAGAGATGT+AGG	-	chr6_1:28655278-28655297	None:intergenic	40.0%
	AGATATCACTCAAAGTGCAG+GGG	-	chr6_1:28655308-28655327	None:intergenic	40.0%
	CAGATATCACTCAAAGTGCA+GGG	-	chr6_1:28655309-28655328	None:intergenic	40.0%
!	ATTGGAGAGTTTGAGATCAG+TGG	-	chr6_1:28655371-28655390	None:intergenic	40.0%
	TATTTGGCAATGCATGGTCT+TGG	+	chr6_1:28655455-28655474	Msa0881460:three_prime_UTR	40.0%
	GCATAAGATCGAGTGAAAGA+AGG	+	chr6_1:28653696-28653715	Msa0881460:five_prime_UTR	40.0%
	GTGACCTTTCTCAGAAATTC+TGG	+	chr6_1:28653988-28654007	Msa0881460:CDS	40.0%
	ACCTTTCTCAGAAATTCTGG+AGG	+	chr6_1:28653991-28654010	Msa0881460:CDS	40.0%
!	ACCTCCAGAATTTCTGAGAA+AGG	-	chr6_1:28653995-28654014	None:intergenic	40.0%
!	TTCTCAGAAATTCTGGAGGT+TGG	+	chr6_1:28653995-28654014	Msa0881460:intron	40.0%
	TCCAACTATCTGTTGTGTGT+AGG	+	chr6_1:28654486-28654505	Msa0881460:intron	40.0%
	TCCTACACACAACAGATAGT+TGG	-	chr6_1:28654490-28654509	None:intergenic	40.0%
	ACTATCTGTTGTGTGTAGGA+TGG	+	chr6_1:28654490-28654509	Msa0881460:intron	40.0%
	CTATCTGTTGTGTGTAGGAT+GGG	+	chr6_1:28654491-28654510	Msa0881460:intron	40.0%
	GCACTGCAAGATTATCAACA+AGG	-	chr6_1:28655107-28655126	None:intergenic	40.0%
	TGCTCTCCTGATTATGCTTA+TGG	+	chr6_1:28655124-28655143	Msa0881460:CDS	40.0%
	CTCAAACTCCAAAACAGAGT+TGG	-	chr6_1:28655182-28655201	None:intergenic	40.0%
	TTGAGTATCCTACATCTCTG+TGG	+	chr6_1:28655267-28655286	Msa0881460:three_prime_UTR	40.0%
	ATACATGTCCACAGAGATGT+AGG	-	chr6_1:28655278-28655297	None:intergenic	40.0%
	AGATATCACTCAAAGTGCAG+GGG	-	chr6_1:28655308-28655327	None:intergenic	40.0%
	CAGATATCACTCAAAGTGCA+GGG	-	chr6_1:28655309-28655328	None:intergenic	40.0%
!	ATTGGAGAGTTTGAGATCAG+TGG	-	chr6_1:28655371-28655390	None:intergenic	40.0%
	TATTTGGCAATGCATGGTCT+TGG	+	chr6_1:28655455-28655474	Msa0881460:three_prime_UTR	40.0%
	CAAATCGTTCGACCTGGAAA+CGG	+	chr6_1:28653744-28653763	Msa0881460:CDS	45.0%
!!	TTTTGACCAGGAGTTGGCTT+AGG	-	chr6_1:28653771-28653790	None:intergenic	45.0%
!	AAAGTGAATGGATTCTGGCC+AGG	-	chr6_1:28654112-28654131	None:intergenic	45.0%
	GGCAAAGGTTCTGTCATCAA+AGG	+	chr6_1:28654139-28654158	Msa0881460:CDS	45.0%
!!	GAGACTTTAGGACTTACGAG+TGG	-	chr6_1:28654291-28654310	None:intergenic	45.0%
	TTGTGTGTAGGATGGGATGA+AGG	+	chr6_1:28654498-28654517	Msa0881460:intron	45.0%
!!	GGTGTCATTGGCATGCAAAT+CGG	+	chr6_1:28654519-28654538	Msa0881460:CDS	45.0%
	TGGATGCGCAGGTCAATTAA+GGG	-	chr6_1:28654859-28654878	None:intergenic	45.0%
	TTGGATGCGCAGGTCAATTA+AGG	-	chr6_1:28654860-28654879	None:intergenic	45.0%
	ATACACGCGACTCGATTAAG+TGG	-	chr6_1:28654950-28654969	None:intergenic	45.0%
	CTAGCGCCATAAGCATAATC+AGG	-	chr6_1:28655133-28655152	None:intergenic	45.0%
	GATTATGCTTATGGCGCTAG+TGG	+	chr6_1:28655133-28655152	Msa0881460:CDS	45.0%
	GCAGATATCACTCAAAGTGC+AGG	-	chr6_1:28655310-28655329	None:intergenic	45.0%
	CAAATCGTTCGACCTGGAAA+CGG	+	chr6_1:28653744-28653763	Msa0881460:CDS	45.0%
!!	TTTTGACCAGGAGTTGGCTT+AGG	-	chr6_1:28653771-28653790	None:intergenic	45.0%
!	AAAGTGAATGGATTCTGGCC+AGG	-	chr6_1:28654112-28654131	None:intergenic	45.0%
	GGCAAAGGTTCTGTCATCAA+AGG	+	chr6_1:28654139-28654158	Msa0881460:CDS	45.0%
!!	GAGACTTTAGGACTTACGAG+TGG	-	chr6_1:28654291-28654310	None:intergenic	45.0%
	TTGTGTGTAGGATGGGATGA+AGG	+	chr6_1:28654498-28654517	Msa0881460:intron	45.0%
!!	GGTGTCATTGGCATGCAAAT+CGG	+	chr6_1:28654519-28654538	Msa0881460:CDS	45.0%
	TGGATGCGCAGGTCAATTAA+GGG	-	chr6_1:28654859-28654878	None:intergenic	45.0%
	TTGGATGCGCAGGTCAATTA+AGG	-	chr6_1:28654860-28654879	None:intergenic	45.0%
	ATACACGCGACTCGATTAAG+TGG	-	chr6_1:28654950-28654969	None:intergenic	45.0%
	CTAGCGCCATAAGCATAATC+AGG	-	chr6_1:28655133-28655152	None:intergenic	45.0%
	GATTATGCTTATGGCGCTAG+TGG	+	chr6_1:28655133-28655152	Msa0881460:CDS	45.0%
	GCAGATATCACTCAAAGTGC+AGG	-	chr6_1:28655310-28655329	None:intergenic	45.0%
!!	AATTTAATTCAAATTATAAA+GGG	-	chr6_1:28653881-28653900	None:intergenic	5.0%
!!	TAATTTAATTCAAATTATAA+AGG	-	chr6_1:28653882-28653901	None:intergenic	5.0%
!!	AATTTAATTCAAATTATAAA+GGG	-	chr6_1:28653881-28653900	None:intergenic	5.0%
!!	TAATTTAATTCAAATTATAA+AGG	-	chr6_1:28653882-28653901	None:intergenic	5.0%
	GAGAAGCAAATCGTTCGACC+TGG	+	chr6_1:28653738-28653757	Msa0881460:CDS	50.0%
!!	GTGACGTTTTGACCAGGAGT+TGG	-	chr6_1:28653777-28653796	None:intergenic	50.0%
!!	TGGACAGTGACGTTTTGACC+AGG	-	chr6_1:28653783-28653802	None:intergenic	50.0%
!!	GGATGGGATGAAGGTGTCAT+TGG	+	chr6_1:28654507-28654526	Msa0881460:intron	50.0%
	TCGGAGAAGTTGCTCGTCTT+CGG	+	chr6_1:28654538-28654557	Msa0881460:CDS	50.0%
	AAGGTCATGTTTGGATGCGC+AGG	-	chr6_1:28654870-28654889	None:intergenic	50.0%
!	TTGGGTTGTATTCCCCAAGC+AGG	-	chr6_1:28655163-28655182	None:intergenic	50.0%
	GAGAAGCAAATCGTTCGACC+TGG	+	chr6_1:28653738-28653757	Msa0881460:CDS	50.0%
!!	GTGACGTTTTGACCAGGAGT+TGG	-	chr6_1:28653777-28653796	None:intergenic	50.0%
!!	TGGACAGTGACGTTTTGACC+AGG	-	chr6_1:28653783-28653802	None:intergenic	50.0%
!!	GGATGGGATGAAGGTGTCAT+TGG	+	chr6_1:28654507-28654526	Msa0881460:intron	50.0%
	TCGGAGAAGTTGCTCGTCTT+CGG	+	chr6_1:28654538-28654557	Msa0881460:CDS	50.0%
	AAGGTCATGTTTGGATGCGC+AGG	-	chr6_1:28654870-28654889	None:intergenic	50.0%
!	TTGGGTTGTATTCCCCAAGC+AGG	-	chr6_1:28655163-28655182	None:intergenic	50.0%
!	GTTGGCTTAGGACCGTTTCC+AGG	-	chr6_1:28653759-28653778	None:intergenic	55.0%
	AACGGTCCTAAGCCAACTCC+TGG	+	chr6_1:28653762-28653781	Msa0881460:CDS	55.0%
	AACGTCACTGTCCACTGCAC+CGG	+	chr6_1:28653789-28653808	Msa0881460:CDS	55.0%
	ACTGTCCACTGCACCGGATA+CGG	+	chr6_1:28653795-28653814	Msa0881460:CDS	55.0%
!!	GAGAGAGTGCTCACCGTATC+CGG	-	chr6_1:28653811-28653830	None:intergenic	55.0%
	CGGAGAAGTTGCTCGTCTTC+GGG	+	chr6_1:28654539-28654558	Msa0881460:CDS	55.0%
	CGCTAGTGGCTTTCCTGCTT+GGG	+	chr6_1:28655147-28655166	Msa0881460:CDS	55.0%
	GCTAGTGGCTTTCCTGCTTG+GGG	+	chr6_1:28655148-28655167	Msa0881460:CDS	55.0%
!	GTTGGCTTAGGACCGTTTCC+AGG	-	chr6_1:28653759-28653778	None:intergenic	55.0%
	AACGGTCCTAAGCCAACTCC+TGG	+	chr6_1:28653762-28653781	Msa0881460:CDS	55.0%
	AACGTCACTGTCCACTGCAC+CGG	+	chr6_1:28653789-28653808	Msa0881460:CDS	55.0%
	ACTGTCCACTGCACCGGATA+CGG	+	chr6_1:28653795-28653814	Msa0881460:CDS	55.0%
!!	GAGAGAGTGCTCACCGTATC+CGG	-	chr6_1:28653811-28653830	None:intergenic	55.0%
	CGGAGAAGTTGCTCGTCTTC+GGG	+	chr6_1:28654539-28654558	Msa0881460:CDS	55.0%
	CGCTAGTGGCTTTCCTGCTT+GGG	+	chr6_1:28655147-28655166	Msa0881460:CDS	55.0%
	GCTAGTGGCTTTCCTGCTTG+GGG	+	chr6_1:28655148-28655167	Msa0881460:CDS	55.0%
	GCGCTAGTGGCTTTCCTGCT+TGG	+	chr6_1:28655146-28655165	Msa0881460:CDS	60.0%
	GCGCTAGTGGCTTTCCTGCT+TGG	+	chr6_1:28655146-28655165	Msa0881460:CDS	60.0%
	GCTCACCGTATCCGGTGCAG+TGG	-	chr6_1:28653803-28653822	None:intergenic	65.0%
	GCTCACCGTATCCGGTGCAG+TGG	-	chr6_1:28653803-28653822	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	28653635	28655550	28653635	ID=Msa0881460;Name=Msa0881460
chr6_1	mRNA	28653635	28655550	28653635	ID=Msa0881460-mRNA-1;Parent=Msa0881460;Name=Msa0881460-mRNA-1;_AED=0.01;_eAED=0.01;_QI=94\|1\|1\|1\|1\|1\|5\|325\|112
chr6_1	exon	28653635	28653816	28653635	ID=Msa0881460-mRNA-1:exon:7258;Parent=Msa0881460-mRNA-1
chr6_1	exon	28653979	28654012	28653979	ID=Msa0881460-mRNA-1:exon:7259;Parent=Msa0881460-mRNA-1
chr6_1	exon	28654099	28654160	28654099	ID=Msa0881460-mRNA-1:exon:7260;Parent=Msa0881460-mRNA-1
chr6_1	exon	28654508	28654560	28654508	ID=Msa0881460-mRNA-1:exon:7261;Parent=Msa0881460-mRNA-1
chr6_1	exon	28655124	28655550	28655124	ID=Msa0881460-mRNA-1:exon:7262;Parent=Msa0881460-mRNA-1
chr6_1	five_prime_UTR	28653635	28653728	28653635	ID=Msa0881460-mRNA-1:five_prime_utr;Parent=Msa0881460-mRNA-1
chr6_1	CDS	28653729	28653816	28653729	ID=Msa0881460-mRNA-1:cds;Parent=Msa0881460-mRNA-1
chr6_1	CDS	28653979	28654012	28653979	ID=Msa0881460-mRNA-1:cds;Parent=Msa0881460-mRNA-1
chr6_1	CDS	28654099	28654160	28654099	ID=Msa0881460-mRNA-1:cds;Parent=Msa0881460-mRNA-1
chr6_1	CDS	28654508	28654560	28654508	ID=Msa0881460-mRNA-1:cds;Parent=Msa0881460-mRNA-1
chr6_1	CDS	28655124	28655225	28655124	ID=Msa0881460-mRNA-1:cds;Parent=Msa0881460-mRNA-1
chr6_1	three_prime_UTR	28655226	28655550	28655226	ID=Msa0881460-mRNA-1:three_prime_utr;Parent=Msa0881460-mRNA-1

Gene Sequence	>Msa0881460 ATGGGAGTTGAGAAGCAAATCGTTCGACCTGGAAACGGTCCTAAGCCAACTCCTGGTCAAAACGTCACTGTCCACTGCACCGGATACGGCAAAAACGGTGACCTTTCTCAGAAATTCTGGAGTACAAAAGATCCTGGCCAGAATCCATTCACTTTCAAAATCGGCAAAGGTTCTGTCATCAAAGGATGGGATGAAGGTGTCATTGGCATGCAAATCGGAGAAGTTGCTCGTCTTCGGTGCTCTCCTGATTATGCTTATGGCGCTAGTGGCTTTCCTGCTTGGGGAATACAACCCAACTCTGTTTTGGAGTTTGAGATTGAGGTCTTGAGTGTAGAATGA
Protein sequence	>Msa0881460 MGVEKQIVRPGNGPKPTPGQNVTVHCTGYGKNGDLSQKFWSTKDPGQNPFTFKIGKGSVIKGWDEGVIGMQIGEVARLRCSPDYAYGASGFPAWGIQPNSVLEFEIEVLSVE*