AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0574240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574240 MtrunA17_Chr8g0390511 93.805 226 12 2 1 225 1 225 6.68e-154 426
Msa0574240 MtrunA17_Chr1g0147191 60.930 215 77 2 4 216 1 210 1.61e-100 290
Msa0574240 MtrunA17_Chr6g0465041 27.000 200 114 3 10 206 55 225 5.15e-21 87.8
Msa0574240 MtrunA17_Chr1g0187331 28.415 183 103 4 7 188 8 163 2.92e-17 76.6
Msa0574240 MtrunA17_Chr7g0255351 26.042 192 114 4 9 200 88 251 4.14e-16 75.1
Msa0574240 MtrunA17_Chr1g0207521 24.623 199 122 4 10 207 84 255 2.80e-14 70.1
Msa0574240 MtrunA17_Chr2g0288811 24.479 192 117 4 9 199 10 174 7.28e-13 64.7
Msa0574240 MtrunA17_Chr2g0324001 24.878 205 124 4 9 210 122 299 5.06e-11 61.2
Msa0574240 MtrunA17_Chr2g0318131 24.510 204 128 3 5 208 174 351 8.74e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574240 AT3G24570.1 62.025 237 71 3 4 224 1 234 1.55e-106 306
Msa0574240 AT3G24570.2 56.962 237 70 4 4 224 1 221 1.57e-92 271
Msa0574240 AT5G43140.1 28.358 201 112 4 10 207 81 252 2.40e-19 84.0
Msa0574240 AT4G04470.1 28.421 190 108 4 11 199 14 176 7.18e-18 78.6
Msa0574240 AT4G04470.2 28.421 190 108 4 11 199 14 176 7.18e-18 78.6
Msa0574240 AT4G14305.1 28.866 194 110 4 7 199 8 174 2.40e-17 77.4
Msa0574240 AT5G19750.1 26.000 200 120 3 9 208 116 287 6.31e-15 72.4
Msa0574240 AT2G14860.1 22.959 196 125 2 10 205 76 245 3.35e-14 70.1
Msa0574240 AT4G33905.1 22.564 195 125 3 10 204 85 253 1.21e-12 65.9
Msa0574240 AT4G14305.2 30.075 133 85 4 69 199 36 162 3.02e-11 60.5
Msa0574240 AT1G52870.2 26.238 202 121 4 8 208 170 344 7.87e-11 61.2

Find 68 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTCCAGTTGGCCATTTC+TGG 0.094401 4_1:+77591940 Msa0574240:CDS
GTTGGATTAGATATTCATTT+TGG 0.174535 4_1:-77591374 None:intergenic
TCGCCACCTCCGGCGTCCTT+TGG 0.232906 4_1:+77591662 Msa0574240:CDS
CGCCACCTCCGGCGTCCTTT+GGG 0.300937 4_1:+77591663 Msa0574240:CDS
AAAACGAAATTTAAAAGTTA+AGG 0.324741 4_1:+77591347 Msa0574240:five_prime_UTR
TGTAAACCTGTTCTGCTTGC+TGG 0.334368 4_1:+77592806 Msa0574240:CDS
TGTCTCTTATCGTATTAATC+TGG 0.342115 4_1:-77591483 None:intergenic
CAACCATTTCATTCTTCAAA+TGG 0.346801 4_1:+77592888 Msa0574240:CDS
GAGGAATTATTTCCCAGCTT+TGG 0.352122 4_1:+77592707 Msa0574240:CDS
ATTAAAATTGAAGAACATCT+TGG 0.355071 4_1:-77593038 None:intergenic
TCCGGCGTCCTTTGGGCTGT+TGG 0.355440 4_1:+77591670 Msa0574240:CDS
TTAAAATTGAAGAACATCTT+GGG 0.371624 4_1:-77593037 None:intergenic
TGGATAGTGCTTTCTTGTCT+TGG 0.372265 4_1:+77592826 Msa0574240:CDS
CGTGTTTCTCTGTCTACTGT+TGG 0.376956 4_1:-77591392 None:intergenic
CACTATCCAGCAAGCAGAAC+AGG 0.391334 4_1:-77592812 None:intergenic
GTTACTAGTATGTTTGGAAT+TGG 0.394362 4_1:+77591912 Msa0574240:CDS
GTTGCTGTTACTAGTATGTT+TGG 0.411075 4_1:+77591906 Msa0574240:CDS
TGATATTGAAGGCATGGAAT+TGG 0.416123 4_1:+77591599 Msa0574240:CDS
TTTCCATTTGAAGAATGAAA+TGG 0.417993 4_1:-77592891 None:intergenic
TAATACGATAAGAGACAGTT+AGG 0.423553 4_1:+77591489 Msa0574240:five_prime_UTR
TAGTGCTTTCTTGTCTTGGT+TGG 0.424910 4_1:+77592830 Msa0574240:CDS
ACTCAGTAATTAATTAATAG+TGG 0.430643 4_1:+77593134 Msa0574240:three_prime_UTR
CCACCTTCTAAAACCAAAGC+TGG 0.434992 4_1:-77592720 None:intergenic
GTCAATGATGATGAAGATAC+TGG 0.437620 4_1:+77592919 Msa0574240:exon
TTTCATTCTTCAAATGGAAA+AGG 0.437632 4_1:+77592894 Msa0574240:CDS
CTCTTGTAGGTATGAAGGCT+TGG 0.440685 4_1:+77592515 Msa0574240:intron
ATTGTAATAGATAGATTCAT+AGG 0.441979 4_1:+77592990 Msa0574240:three_prime_UTR
ACTTACATGGGATTGTCTGC+TGG 0.443240 4_1:+77592651 Msa0574240:CDS
TTCATCTCTTGTAGGTATGA+AGG 0.445708 4_1:+77592510 Msa0574240:intron
GTGGCGACTTGGGTCTTCAC+TGG 0.448316 4_1:-77591646 None:intergenic
TTCAAGTCTTCCTTCACCTC+AGG 0.449281 4_1:-77592684 None:intergenic
ATGATAAATTTGTCATCGAT+TGG 0.450538 4_1:+77591877 Msa0574240:CDS
AGCCCAAAGGACGCCGGAGG+TGG 0.459956 4_1:-77591665 None:intergenic
AAAGATGTCGATGATATTGA+AGG 0.473579 4_1:+77591588 Msa0574240:exon
GACGCCGGAGGTGGCGACTT+GGG 0.475559 4_1:-77591656 None:intergenic
GCTACGGATCGTGCAGTTTG+TGG 0.477640 4_1:-77592570 None:intergenic
TGGTGTGTGGCCAATTGTGC+AGG 0.479891 4_1:+77592740 Msa0574240:CDS
AAGACCCAAGTCGCCACCTC+CGG 0.484846 4_1:+77591652 Msa0574240:CDS
ACATACCAGAAATGGCCAAC+TGG 0.486779 4_1:-77591945 None:intergenic
CGTATTAATCTGGATTTCGT+TGG 0.489512 4_1:-77591473 None:intergenic
TACAATTTCTAAAACATCAC+AGG 0.499099 4_1:-77592973 None:intergenic
GGACGCCGGAGGTGGCGACT+TGG 0.499705 4_1:-77591657 None:intergenic
CGAAATTCTGTGCATGTGTA+TGG 0.503898 4_1:-77593110 None:intergenic
CATTCTTCAAATGGAAAAGG+TGG 0.505391 4_1:+77592897 Msa0574240:CDS
TACAGAAGCTGGTACTTGAC+AGG 0.517117 4_1:-77592783 None:intergenic
TAAAATTGAAGAACATCTTG+GGG 0.539160 4_1:-77593036 None:intergenic
AGCAACAGAAAGATGCAGCT+TGG 0.545356 4_1:+77592853 Msa0574240:CDS
ATAACTAATTGCCGAGGACG+TGG 0.546264 4_1:-77591515 None:intergenic
AAAGACTATTCCTGAGGTGA+AGG 0.559012 4_1:+77592674 Msa0574240:CDS
ACAGGTTTACATACAGAAGC+TGG 0.562212 4_1:-77592794 None:intergenic
TGTCGATGATATTGAAGGCA+TGG 0.565785 4_1:+77591593 Msa0574240:CDS
CAGTTAGGTTCCCACGTCCT+CGG 0.576952 4_1:+77591504 Msa0574240:five_prime_UTR
AAGATGCAGCTTGGAAACAA+TGG 0.578706 4_1:+77592862 Msa0574240:CDS
TCCAACAGCCCAAAGGACGC+CGG 0.588788 4_1:-77591671 None:intergenic
CACCTTCTAAAACCAAAGCT+GGG 0.592808 4_1:-77592719 None:intergenic
AGGTGAAGGAAGACTTGAAG+AGG 0.593785 4_1:+77592688 Msa0574240:CDS
CGTAGCTACAAAAGTTGCAA+TGG 0.595127 4_1:+77592587 Msa0574240:CDS
AAGAAAACAAACAAATGCAC+CGG 0.610944 4_1:-77592627 None:intergenic
GCTACAAAAGTTGCAATGGA+CGG 0.615386 4_1:+77592591 Msa0574240:CDS
AACAGCCCAAAGGACGCCGG+AGG 0.618772 4_1:-77591668 None:intergenic
TGCTCAGTACATCACTCACT+CGG 0.629668 4_1:+77591702 Msa0574240:CDS
TAACTAATTGCCGAGGACGT+GGG 0.649745 4_1:-77591514 None:intergenic
GATGAAGATACTGGAAACAA+CGG 0.663149 4_1:+77592928 Msa0574240:three_prime_UTR
TCTTCACTGGATGAATTGAG+AGG 0.675816 4_1:-77591633 None:intergenic
TGACATCTCCAACAGCCCAA+AGG 0.677959 4_1:-77591678 None:intergenic
AAAATTGAAGAACATCTTGG+GGG 0.684075 4_1:-77593035 None:intergenic
ACAACTATAACTAATTGCCG+AGG 0.697334 4_1:-77591521 None:intergenic
TGCTGGAAAGACTATTCCTG+AGG 0.700231 4_1:+77592668 Msa0574240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACGAAATTTAAAAGTTA+AGG + chr4_1:77591347-77591366 Msa0574240:five_prime_UTR 15.0%
!! TTTATAATATTTGTAATTGC+AGG + chr4_1:77591842-77591861 Msa0574240:intron 15.0%
!! AAAACGAAATTTAAAAGTTA+AGG + chr4_1:77591347-77591366 Msa0574240:five_prime_UTR 15.0%
!! TTTATAATATTTGTAATTGC+AGG + chr4_1:77591842-77591861 Msa0574240:intron 15.0%
!! GCAAAAATTGTAATGTAAAT+AGG + chr4_1:77591450-77591469 Msa0574240:five_prime_UTR 20.0%
!!! TTTGTTTTCTTTACTTACAT+GGG + chr4_1:77592639-77592658 Msa0574240:CDS 20.0%
!! ATTGTAATAGATAGATTCAT+AGG + chr4_1:77592990-77593009 Msa0574240:three_prime_UTR 20.0%
!! TTAAAATTGAAGAACATCTT+GGG - chr4_1:77593040-77593059 None:intergenic 20.0%
!! ATTAAAATTGAAGAACATCT+TGG - chr4_1:77593041-77593060 None:intergenic 20.0%
!!! TTCTACTCTTTTAATCTTTT+CGG - chr4_1:77593079-77593098 None:intergenic 20.0%
!! ACTCAGTAATTAATTAATAG+TGG + chr4_1:77593134-77593153 Msa0574240:three_prime_UTR 20.0%
!! GCAAAAATTGTAATGTAAAT+AGG + chr4_1:77591450-77591469 Msa0574240:five_prime_UTR 20.0%
!!! TTTGTTTTCTTTACTTACAT+GGG + chr4_1:77592639-77592658 Msa0574240:CDS 20.0%
!! ATTGTAATAGATAGATTCAT+AGG + chr4_1:77592990-77593009 Msa0574240:three_prime_UTR 20.0%
!! TTAAAATTGAAGAACATCTT+GGG - chr4_1:77593040-77593059 None:intergenic 20.0%
!! ATTAAAATTGAAGAACATCT+TGG - chr4_1:77593041-77593060 None:intergenic 20.0%
!!! TTCTACTCTTTTAATCTTTT+CGG - chr4_1:77593079-77593098 None:intergenic 20.0%
!! ACTCAGTAATTAATTAATAG+TGG + chr4_1:77593134-77593153 Msa0574240:three_prime_UTR 20.0%
!!! GTTGGATTAGATATTCATTT+TGG - chr4_1:77591377-77591396 None:intergenic 25.0%
!! ACATCTTTTCTATTCTATTC+AGG - chr4_1:77591576-77591595 None:intergenic 25.0%
! AAAGAGGGTAATATAGAAAT+GGG - chr4_1:77591789-77591808 None:intergenic 25.0%
! ATGATAAATTTGTCATCGAT+TGG + chr4_1:77591877-77591896 Msa0574240:CDS 25.0%
! AAGAAAAAACATACCAGAAA+TGG - chr4_1:77591956-77591975 None:intergenic 25.0%
!! ATGCTGTTTATTTACATTAC+AGG + chr4_1:77591997-77592016 Msa0574240:intron 25.0%
!! AACTTCACATTTTTGTTCAA+AGG + chr4_1:77592070-77592089 Msa0574240:intron 25.0%
!!! ATTTACAAGCTGATTTTGTA+AGG + chr4_1:77592171-77592190 Msa0574240:intron 25.0%
!!! TTTACAAGCTGATTTTGTAA+GGG + chr4_1:77592172-77592191 Msa0574240:intron 25.0%
! TTAACTCAATTACAGAAAAC+CGG - chr4_1:77592306-77592325 None:intergenic 25.0%
! ATTCAGTTTACTAAGAAGAT+TGG + chr4_1:77592403-77592422 Msa0574240:intron 25.0%
!!! GTTTGTTTTCTTTACTTACA+TGG + chr4_1:77592638-77592657 Msa0574240:CDS 25.0%
! AAATTAAAAACCTGCACAAT+TGG - chr4_1:77592753-77592772 None:intergenic 25.0%
!!! TTGTGCAGGTTTTTAATTTT+CGG + chr4_1:77592754-77592773 Msa0574240:CDS 25.0%
! TTTCCATTTGAAGAATGAAA+TGG - chr4_1:77592894-77592913 None:intergenic 25.0%
! TTTCATTCTTCAAATGGAAA+AGG + chr4_1:77592894-77592913 Msa0574240:CDS 25.0%
! TCACAGGAAAAATTATGAAA+TGG - chr4_1:77592960-77592979 None:intergenic 25.0%
!!! TACAATTTCTAAAACATCAC+AGG - chr4_1:77592976-77592995 None:intergenic 25.0%
! TAAAATTGAAGAACATCTTG+GGG - chr4_1:77593039-77593058 None:intergenic 25.0%
!! TCTACTCTTTTAATCTTTTC+GGG - chr4_1:77593078-77593097 None:intergenic 25.0%
!!! GTTGGATTAGATATTCATTT+TGG - chr4_1:77591377-77591396 None:intergenic 25.0%
!! ACATCTTTTCTATTCTATTC+AGG - chr4_1:77591576-77591595 None:intergenic 25.0%
! AAAGAGGGTAATATAGAAAT+GGG - chr4_1:77591789-77591808 None:intergenic 25.0%
! ATGATAAATTTGTCATCGAT+TGG + chr4_1:77591877-77591896 Msa0574240:CDS 25.0%
! AAGAAAAAACATACCAGAAA+TGG - chr4_1:77591956-77591975 None:intergenic 25.0%
!! ATGCTGTTTATTTACATTAC+AGG + chr4_1:77591997-77592016 Msa0574240:intron 25.0%
!! AACTTCACATTTTTGTTCAA+AGG + chr4_1:77592070-77592089 Msa0574240:intron 25.0%
!!! ATTTACAAGCTGATTTTGTA+AGG + chr4_1:77592171-77592190 Msa0574240:intron 25.0%
!!! TTTACAAGCTGATTTTGTAA+GGG + chr4_1:77592172-77592191 Msa0574240:intron 25.0%
! TTAACTCAATTACAGAAAAC+CGG - chr4_1:77592306-77592325 None:intergenic 25.0%
! ATTCAGTTTACTAAGAAGAT+TGG + chr4_1:77592403-77592422 Msa0574240:intron 25.0%
!!! GTTTGTTTTCTTTACTTACA+TGG + chr4_1:77592638-77592657 Msa0574240:CDS 25.0%
! AAATTAAAAACCTGCACAAT+TGG - chr4_1:77592753-77592772 None:intergenic 25.0%
!!! TTGTGCAGGTTTTTAATTTT+CGG + chr4_1:77592754-77592773 Msa0574240:CDS 25.0%
! TTTCCATTTGAAGAATGAAA+TGG - chr4_1:77592894-77592913 None:intergenic 25.0%
! TTTCATTCTTCAAATGGAAA+AGG + chr4_1:77592894-77592913 Msa0574240:CDS 25.0%
! TCACAGGAAAAATTATGAAA+TGG - chr4_1:77592960-77592979 None:intergenic 25.0%
!!! TACAATTTCTAAAACATCAC+AGG - chr4_1:77592976-77592995 None:intergenic 25.0%
! TAAAATTGAAGAACATCTTG+GGG - chr4_1:77593039-77593058 None:intergenic 25.0%
!! TCTACTCTTTTAATCTTTTC+GGG - chr4_1:77593078-77593097 None:intergenic 25.0%
TGTCTCTTATCGTATTAATC+TGG - chr4_1:77591486-77591505 None:intergenic 30.0%
TAATACGATAAGAGACAGTT+AGG + chr4_1:77591489-77591508 Msa0574240:five_prime_UTR 30.0%
AAAGATGTCGATGATATTGA+AGG + chr4_1:77591588-77591607 Msa0574240:exon 30.0%
GAAAGAGGGTAATATAGAAA+TGG - chr4_1:77591790-77591809 None:intergenic 30.0%
! GTTACTAGTATGTTTGGAAT+TGG + chr4_1:77591912-77591931 Msa0574240:CDS 30.0%
!!! ATGTTTGGAATTGGTTTTGT+TGG + chr4_1:77591921-77591940 Msa0574240:CDS 30.0%
AGTTAACAACAATTTGACAG+AGG - chr4_1:77592054-77592073 None:intergenic 30.0%
GGTCAATATCTTATTCAATG+TGG - chr4_1:77592115-77592134 None:intergenic 30.0%
!! CTACTTGTAAAGTCATTTTC+AGG - chr4_1:77592136-77592155 None:intergenic 30.0%
CTGAAAATGACTTTACAAGT+AGG + chr4_1:77592134-77592153 Msa0574240:intron 30.0%
CAAAATCAGCTTGTAAATGA+GGG - chr4_1:77592169-77592188 None:intergenic 30.0%
ACAAAATCAGCTTGTAAATG+AGG - chr4_1:77592170-77592189 None:intergenic 30.0%
! GATTTTGTAAGGGTTGAATT+TGG + chr4_1:77592182-77592201 Msa0574240:intron 30.0%
CTAATACCATCTTAGAATCT+AGG - chr4_1:77592213-77592232 None:intergenic 30.0%
! CGGTGATTTCATAAGATTTT+GGG + chr4_1:77592350-77592369 Msa0574240:intron 30.0%
! TGATTTCATAAGATTTTGGG+AGG + chr4_1:77592353-77592372 Msa0574240:intron 30.0%
!! GATTTCATAAGATTTTGGGA+GGG + chr4_1:77592354-77592373 Msa0574240:intron 30.0%
TTGTAATTTCCTGCTACAAA+AGG + chr4_1:77592457-77592476 Msa0574240:intron 30.0%
AAGAAAACAAACAAATGCAC+CGG - chr4_1:77592630-77592649 None:intergenic 30.0%
CAACCATTTCATTCTTCAAA+TGG + chr4_1:77592888-77592907 Msa0574240:CDS 30.0%
AAAATTGAAGAACATCTTGG+GGG - chr4_1:77593038-77593057 None:intergenic 30.0%
TGTCTCTTATCGTATTAATC+TGG - chr4_1:77591486-77591505 None:intergenic 30.0%
TAATACGATAAGAGACAGTT+AGG + chr4_1:77591489-77591508 Msa0574240:five_prime_UTR 30.0%
AAAGATGTCGATGATATTGA+AGG + chr4_1:77591588-77591607 Msa0574240:exon 30.0%
GAAAGAGGGTAATATAGAAA+TGG - chr4_1:77591790-77591809 None:intergenic 30.0%
! GTTACTAGTATGTTTGGAAT+TGG + chr4_1:77591912-77591931 Msa0574240:CDS 30.0%
!!! ATGTTTGGAATTGGTTTTGT+TGG + chr4_1:77591921-77591940 Msa0574240:CDS 30.0%
AGTTAACAACAATTTGACAG+AGG - chr4_1:77592054-77592073 None:intergenic 30.0%
GGTCAATATCTTATTCAATG+TGG - chr4_1:77592115-77592134 None:intergenic 30.0%
!! CTACTTGTAAAGTCATTTTC+AGG - chr4_1:77592136-77592155 None:intergenic 30.0%
CTGAAAATGACTTTACAAGT+AGG + chr4_1:77592134-77592153 Msa0574240:intron 30.0%
CAAAATCAGCTTGTAAATGA+GGG - chr4_1:77592169-77592188 None:intergenic 30.0%
ACAAAATCAGCTTGTAAATG+AGG - chr4_1:77592170-77592189 None:intergenic 30.0%
! GATTTTGTAAGGGTTGAATT+TGG + chr4_1:77592182-77592201 Msa0574240:intron 30.0%
CTAATACCATCTTAGAATCT+AGG - chr4_1:77592213-77592232 None:intergenic 30.0%
! CGGTGATTTCATAAGATTTT+GGG + chr4_1:77592350-77592369 Msa0574240:intron 30.0%
! TGATTTCATAAGATTTTGGG+AGG + chr4_1:77592353-77592372 Msa0574240:intron 30.0%
!! GATTTCATAAGATTTTGGGA+GGG + chr4_1:77592354-77592373 Msa0574240:intron 30.0%
TTGTAATTTCCTGCTACAAA+AGG + chr4_1:77592457-77592476 Msa0574240:intron 30.0%
AAGAAAACAAACAAATGCAC+CGG - chr4_1:77592630-77592649 None:intergenic 30.0%
CAACCATTTCATTCTTCAAA+TGG + chr4_1:77592888-77592907 Msa0574240:CDS 30.0%
AAAATTGAAGAACATCTTGG+GGG - chr4_1:77593038-77593057 None:intergenic 30.0%
CGTATTAATCTGGATTTCGT+TGG - chr4_1:77591476-77591495 None:intergenic 35.0%
ACAACTATAACTAATTGCCG+AGG - chr4_1:77591524-77591543 None:intergenic 35.0%
! TGATATTGAAGGCATGGAAT+TGG + chr4_1:77591599-77591618 Msa0574240:CDS 35.0%
! TGGGTATCGTTAAAGACTTA+CGG - chr4_1:77591770-77591789 None:intergenic 35.0%
! GTTGCTGTTACTAGTATGTT+TGG + chr4_1:77591906-77591925 Msa0574240:CDS 35.0%
! TACATTACAGGCTTTCTAGT+TGG + chr4_1:77592009-77592028 Msa0574240:intron 35.0%
AGAACCTATACAAAGTCTGT+TGG + chr4_1:77592231-77592250 Msa0574240:intron 35.0%
GAACCTATACAAAGTCTGTT+GGG + chr4_1:77592232-77592251 Msa0574240:intron 35.0%
!! AACCCAAATTCTAAGACTAG+CGG + chr4_1:77592330-77592349 Msa0574240:intron 35.0%
! GCGGTGATTTCATAAGATTT+TGG + chr4_1:77592349-77592368 Msa0574240:intron 35.0%
! CATACAAATCCTTTTGTAGC+AGG - chr4_1:77592469-77592488 None:intergenic 35.0%
TTCATACCTACAAGAGATGA+AGG - chr4_1:77592511-77592530 None:intergenic 35.0%
TTCATCTCTTGTAGGTATGA+AGG + chr4_1:77592510-77592529 Msa0574240:intron 35.0%
! CATTGCAACTTTTGTAGCTA+CGG - chr4_1:77592589-77592608 None:intergenic 35.0%
CATTCTTCAAATGGAAAAGG+TGG + chr4_1:77592897-77592916 Msa0574240:CDS 35.0%
! GTCAATGATGATGAAGATAC+TGG + chr4_1:77592919-77592938 Msa0574240:exon 35.0%
GATGAAGATACTGGAAACAA+CGG + chr4_1:77592928-77592947 Msa0574240:three_prime_UTR 35.0%
CGTATTAATCTGGATTTCGT+TGG - chr4_1:77591476-77591495 None:intergenic 35.0%
ACAACTATAACTAATTGCCG+AGG - chr4_1:77591524-77591543 None:intergenic 35.0%
! TGATATTGAAGGCATGGAAT+TGG + chr4_1:77591599-77591618 Msa0574240:CDS 35.0%
! TGGGTATCGTTAAAGACTTA+CGG - chr4_1:77591770-77591789 None:intergenic 35.0%
! GTTGCTGTTACTAGTATGTT+TGG + chr4_1:77591906-77591925 Msa0574240:CDS 35.0%
! TACATTACAGGCTTTCTAGT+TGG + chr4_1:77592009-77592028 Msa0574240:intron 35.0%
AGAACCTATACAAAGTCTGT+TGG + chr4_1:77592231-77592250 Msa0574240:intron 35.0%
GAACCTATACAAAGTCTGTT+GGG + chr4_1:77592232-77592251 Msa0574240:intron 35.0%
!! AACCCAAATTCTAAGACTAG+CGG + chr4_1:77592330-77592349 Msa0574240:intron 35.0%
! GCGGTGATTTCATAAGATTT+TGG + chr4_1:77592349-77592368 Msa0574240:intron 35.0%
! CATACAAATCCTTTTGTAGC+AGG - chr4_1:77592469-77592488 None:intergenic 35.0%
TTCATACCTACAAGAGATGA+AGG - chr4_1:77592511-77592530 None:intergenic 35.0%
TTCATCTCTTGTAGGTATGA+AGG + chr4_1:77592510-77592529 Msa0574240:intron 35.0%
! CATTGCAACTTTTGTAGCTA+CGG - chr4_1:77592589-77592608 None:intergenic 35.0%
CATTCTTCAAATGGAAAAGG+TGG + chr4_1:77592897-77592916 Msa0574240:CDS 35.0%
! GTCAATGATGATGAAGATAC+TGG + chr4_1:77592919-77592938 Msa0574240:exon 35.0%
GATGAAGATACTGGAAACAA+CGG + chr4_1:77592928-77592947 Msa0574240:three_prime_UTR 35.0%
TGTCGATGATATTGAAGGCA+TGG + chr4_1:77591593-77591612 Msa0574240:CDS 40.0%
TCTTCACTGGATGAATTGAG+AGG - chr4_1:77591636-77591655 None:intergenic 40.0%
GTATCGTTAAAGACTTACGG+TGG - chr4_1:77591767-77591786 None:intergenic 40.0%
!!! ATTGGTTTTGTTGGTCCAGT+TGG + chr4_1:77591930-77591949 Msa0574240:CDS 40.0%
! GCTCAACCTAGATTCTAAGA+TGG + chr4_1:77592204-77592223 Msa0574240:intron 40.0%
GATTCCTTCATCTTACAAGC+CGG + chr4_1:77592284-77592303 Msa0574240:intron 40.0%
!! AAAACCGGCTTGTAAGATGA+AGG - chr4_1:77592291-77592310 None:intergenic 40.0%
!! CACCGCTAGTCTTAGAATTT+GGG - chr4_1:77592335-77592354 None:intergenic 40.0%
!! TCACCGCTAGTCTTAGAATT+TGG - chr4_1:77592336-77592355 None:intergenic 40.0%
AACCAACCTTCATCTCTTGT+AGG + chr4_1:77592502-77592521 Msa0574240:intron 40.0%
TACCTACAAGAGATGAAGGT+TGG - chr4_1:77592507-77592526 None:intergenic 40.0%
CGTAGCTACAAAAGTTGCAA+TGG + chr4_1:77592587-77592606 Msa0574240:CDS 40.0%
GCTACAAAAGTTGCAATGGA+CGG + chr4_1:77592591-77592610 Msa0574240:CDS 40.0%
AAAGACTATTCCTGAGGTGA+AGG + chr4_1:77592674-77592693 Msa0574240:CDS 40.0%
GAGGAATTATTTCCCAGCTT+TGG + chr4_1:77592707-77592726 Msa0574240:CDS 40.0%
!!! TTCCCAGCTTTGGTTTTAGA+AGG + chr4_1:77592717-77592736 Msa0574240:CDS 40.0%
!! CACCTTCTAAAACCAAAGCT+GGG - chr4_1:77592722-77592741 None:intergenic 40.0%
ACAGGTTTACATACAGAAGC+TGG - chr4_1:77592797-77592816 None:intergenic 40.0%
TGGATAGTGCTTTCTTGTCT+TGG + chr4_1:77592826-77592845 Msa0574240:CDS 40.0%
TAGTGCTTTCTTGTCTTGGT+TGG + chr4_1:77592830-77592849 Msa0574240:CDS 40.0%
AAGATGCAGCTTGGAAACAA+TGG + chr4_1:77592862-77592881 Msa0574240:CDS 40.0%
CGAAATTCTGTGCATGTGTA+TGG - chr4_1:77593113-77593132 None:intergenic 40.0%
TGTCGATGATATTGAAGGCA+TGG + chr4_1:77591593-77591612 Msa0574240:CDS 40.0%
TCTTCACTGGATGAATTGAG+AGG - chr4_1:77591636-77591655 None:intergenic 40.0%
GTATCGTTAAAGACTTACGG+TGG - chr4_1:77591767-77591786 None:intergenic 40.0%
!!! ATTGGTTTTGTTGGTCCAGT+TGG + chr4_1:77591930-77591949 Msa0574240:CDS 40.0%
! GCTCAACCTAGATTCTAAGA+TGG + chr4_1:77592204-77592223 Msa0574240:intron 40.0%
GATTCCTTCATCTTACAAGC+CGG + chr4_1:77592284-77592303 Msa0574240:intron 40.0%
!! AAAACCGGCTTGTAAGATGA+AGG - chr4_1:77592291-77592310 None:intergenic 40.0%
!! CACCGCTAGTCTTAGAATTT+GGG - chr4_1:77592335-77592354 None:intergenic 40.0%
!! TCACCGCTAGTCTTAGAATT+TGG - chr4_1:77592336-77592355 None:intergenic 40.0%
AACCAACCTTCATCTCTTGT+AGG + chr4_1:77592502-77592521 Msa0574240:intron 40.0%
TACCTACAAGAGATGAAGGT+TGG - chr4_1:77592507-77592526 None:intergenic 40.0%
CGTAGCTACAAAAGTTGCAA+TGG + chr4_1:77592587-77592606 Msa0574240:CDS 40.0%
GCTACAAAAGTTGCAATGGA+CGG + chr4_1:77592591-77592610 Msa0574240:CDS 40.0%
AAAGACTATTCCTGAGGTGA+AGG + chr4_1:77592674-77592693 Msa0574240:CDS 40.0%
GAGGAATTATTTCCCAGCTT+TGG + chr4_1:77592707-77592726 Msa0574240:CDS 40.0%
!!! TTCCCAGCTTTGGTTTTAGA+AGG + chr4_1:77592717-77592736 Msa0574240:CDS 40.0%
!! CACCTTCTAAAACCAAAGCT+GGG - chr4_1:77592722-77592741 None:intergenic 40.0%
ACAGGTTTACATACAGAAGC+TGG - chr4_1:77592797-77592816 None:intergenic 40.0%
TGGATAGTGCTTTCTTGTCT+TGG + chr4_1:77592826-77592845 Msa0574240:CDS 40.0%
TAGTGCTTTCTTGTCTTGGT+TGG + chr4_1:77592830-77592849 Msa0574240:CDS 40.0%
AAGATGCAGCTTGGAAACAA+TGG + chr4_1:77592862-77592881 Msa0574240:CDS 40.0%
CGAAATTCTGTGCATGTGTA+TGG - chr4_1:77593113-77593132 None:intergenic 40.0%
CGTGTTTCTCTGTCTACTGT+TGG - chr4_1:77591395-77591414 None:intergenic 45.0%
TAACTAATTGCCGAGGACGT+GGG - chr4_1:77591517-77591536 None:intergenic 45.0%
ATAACTAATTGCCGAGGACG+TGG - chr4_1:77591518-77591537 None:intergenic 45.0%
TGCTCAGTACATCACTCACT+CGG + chr4_1:77591702-77591721 Msa0574240:CDS 45.0%
GACAGACAGAGAGAGAAAGA+GGG - chr4_1:77591804-77591823 None:intergenic 45.0%
AGACAGACAGAGAGAGAAAG+AGG - chr4_1:77591805-77591824 None:intergenic 45.0%
ACATACCAGAAATGGCCAAC+TGG - chr4_1:77591948-77591967 None:intergenic 45.0%
TGGCCCAACAGACTTTGTAT+AGG - chr4_1:77592238-77592257 None:intergenic 45.0%
CTCTTGTAGGTATGAAGGCT+TGG + chr4_1:77592515-77592534 Msa0574240:intron 45.0%
! GCAATGGACGGCTTGATTTT+CGG + chr4_1:77592603-77592622 Msa0574240:CDS 45.0%
ACTTACATGGGATTGTCTGC+TGG + chr4_1:77592651-77592670 Msa0574240:CDS 45.0%
TGCTGGAAAGACTATTCCTG+AGG + chr4_1:77592668-77592687 Msa0574240:CDS 45.0%
TTCAAGTCTTCCTTCACCTC+AGG - chr4_1:77592687-77592706 None:intergenic 45.0%
AGGTGAAGGAAGACTTGAAG+AGG + chr4_1:77592688-77592707 Msa0574240:CDS 45.0%
!! CCACCTTCTAAAACCAAAGC+TGG - chr4_1:77592723-77592742 None:intergenic 45.0%
!!! CCAGCTTTGGTTTTAGAAGG+TGG + chr4_1:77592720-77592739 Msa0574240:CDS 45.0%
!!! TGGTTTTAGAAGGTGGTGTG+TGG + chr4_1:77592727-77592746 Msa0574240:CDS 45.0%
TACAGAAGCTGGTACTTGAC+AGG - chr4_1:77592786-77592805 None:intergenic 45.0%
TGTAAACCTGTTCTGCTTGC+TGG + chr4_1:77592806-77592825 Msa0574240:CDS 45.0%
AGCAACAGAAAGATGCAGCT+TGG + chr4_1:77592853-77592872 Msa0574240:CDS 45.0%
CGTGTTTCTCTGTCTACTGT+TGG - chr4_1:77591395-77591414 None:intergenic 45.0%
TAACTAATTGCCGAGGACGT+GGG - chr4_1:77591517-77591536 None:intergenic 45.0%
ATAACTAATTGCCGAGGACG+TGG - chr4_1:77591518-77591537 None:intergenic 45.0%
TGCTCAGTACATCACTCACT+CGG + chr4_1:77591702-77591721 Msa0574240:CDS 45.0%
GACAGACAGAGAGAGAAAGA+GGG - chr4_1:77591804-77591823 None:intergenic 45.0%
AGACAGACAGAGAGAGAAAG+AGG - chr4_1:77591805-77591824 None:intergenic 45.0%
ACATACCAGAAATGGCCAAC+TGG - chr4_1:77591948-77591967 None:intergenic 45.0%
TGGCCCAACAGACTTTGTAT+AGG - chr4_1:77592238-77592257 None:intergenic 45.0%
CTCTTGTAGGTATGAAGGCT+TGG + chr4_1:77592515-77592534 Msa0574240:intron 45.0%
! GCAATGGACGGCTTGATTTT+CGG + chr4_1:77592603-77592622 Msa0574240:CDS 45.0%
ACTTACATGGGATTGTCTGC+TGG + chr4_1:77592651-77592670 Msa0574240:CDS 45.0%
TGCTGGAAAGACTATTCCTG+AGG + chr4_1:77592668-77592687 Msa0574240:CDS 45.0%
TTCAAGTCTTCCTTCACCTC+AGG - chr4_1:77592687-77592706 None:intergenic 45.0%
AGGTGAAGGAAGACTTGAAG+AGG + chr4_1:77592688-77592707 Msa0574240:CDS 45.0%
!! CCACCTTCTAAAACCAAAGC+TGG - chr4_1:77592723-77592742 None:intergenic 45.0%
!!! CCAGCTTTGGTTTTAGAAGG+TGG + chr4_1:77592720-77592739 Msa0574240:CDS 45.0%
!!! TGGTTTTAGAAGGTGGTGTG+TGG + chr4_1:77592727-77592746 Msa0574240:CDS 45.0%
TACAGAAGCTGGTACTTGAC+AGG - chr4_1:77592786-77592805 None:intergenic 45.0%
TGTAAACCTGTTCTGCTTGC+TGG + chr4_1:77592806-77592825 Msa0574240:CDS 45.0%
AGCAACAGAAAGATGCAGCT+TGG + chr4_1:77592853-77592872 Msa0574240:CDS 45.0%
TGACATCTCCAACAGCCCAA+AGG - chr4_1:77591681-77591700 None:intergenic 50.0%
TTGGTCCAGTTGGCCATTTC+TGG + chr4_1:77591940-77591959 Msa0574240:CDS 50.0%
CACTATCCAGCAAGCAGAAC+AGG - chr4_1:77592815-77592834 None:intergenic 50.0%
TGACATCTCCAACAGCCCAA+AGG - chr4_1:77591681-77591700 None:intergenic 50.0%
TTGGTCCAGTTGGCCATTTC+TGG + chr4_1:77591940-77591959 Msa0574240:CDS 50.0%
CACTATCCAGCAAGCAGAAC+AGG - chr4_1:77592815-77592834 None:intergenic 50.0%
CAGTTAGGTTCCCACGTCCT+CGG + chr4_1:77591504-77591523 Msa0574240:five_prime_UTR 55.0%
TCTGTTGGGCCACCAGCTAT+CGG + chr4_1:77592246-77592265 Msa0574240:intron 55.0%
! TGGGAGGGTTCTGCTTTGAG+AGG + chr4_1:77592369-77592388 Msa0574240:intron 55.0%
!! GGGAGGGTTCTGCTTTGAGA+GGG + chr4_1:77592370-77592389 Msa0574240:intron 55.0%
GCTACGGATCGTGCAGTTTG+TGG - chr4_1:77592573-77592592 None:intergenic 55.0%
! GGACGGCTTGATTTTCGGTC+CGG + chr4_1:77592608-77592627 Msa0574240:CDS 55.0%
TGGTGTGTGGCCAATTGTGC+AGG + chr4_1:77592740-77592759 Msa0574240:CDS 55.0%
CAGTTAGGTTCCCACGTCCT+CGG + chr4_1:77591504-77591523 Msa0574240:five_prime_UTR 55.0%
TCTGTTGGGCCACCAGCTAT+CGG + chr4_1:77592246-77592265 Msa0574240:intron 55.0%
! TGGGAGGGTTCTGCTTTGAG+AGG + chr4_1:77592369-77592388 Msa0574240:intron 55.0%
!! GGGAGGGTTCTGCTTTGAGA+GGG + chr4_1:77592370-77592389 Msa0574240:intron 55.0%
GCTACGGATCGTGCAGTTTG+TGG - chr4_1:77592573-77592592 None:intergenic 55.0%
! GGACGGCTTGATTTTCGGTC+CGG + chr4_1:77592608-77592627 Msa0574240:CDS 55.0%
TGGTGTGTGGCCAATTGTGC+AGG + chr4_1:77592740-77592759 Msa0574240:CDS 55.0%
! GTGGCGACTTGGGTCTTCAC+TGG - chr4_1:77591649-77591668 None:intergenic 60.0%
AAGACCCAAGTCGCCACCTC+CGG + chr4_1:77591652-77591671 Msa0574240:CDS 60.0%
TCCAACAGCCCAAAGGACGC+CGG - chr4_1:77591674-77591693 None:intergenic 60.0%
CTGTTGGGCCACCAGCTATC+GGG + chr4_1:77592247-77592266 Msa0574240:intron 60.0%
TCGCATGACCCGATAGCTGG+TGG - chr4_1:77592258-77592277 None:intergenic 60.0%
ACCAGCTATCGGGTCATGCG+AGG + chr4_1:77592257-77592276 Msa0574240:intron 60.0%
CCAGCTATCGGGTCATGCGA+GGG + chr4_1:77592258-77592277 Msa0574240:intron 60.0%
CAGCTATCGGGTCATGCGAG+GGG + chr4_1:77592259-77592278 Msa0574240:intron 60.0%
! GTGGCGACTTGGGTCTTCAC+TGG - chr4_1:77591649-77591668 None:intergenic 60.0%
AAGACCCAAGTCGCCACCTC+CGG + chr4_1:77591652-77591671 Msa0574240:CDS 60.0%
TCCAACAGCCCAAAGGACGC+CGG - chr4_1:77591674-77591693 None:intergenic 60.0%
CTGTTGGGCCACCAGCTATC+GGG + chr4_1:77592247-77592266 Msa0574240:intron 60.0%
TCGCATGACCCGATAGCTGG+TGG - chr4_1:77592258-77592277 None:intergenic 60.0%
ACCAGCTATCGGGTCATGCG+AGG + chr4_1:77592257-77592276 Msa0574240:intron 60.0%
CCAGCTATCGGGTCATGCGA+GGG + chr4_1:77592258-77592277 Msa0574240:intron 60.0%
CAGCTATCGGGTCATGCGAG+GGG + chr4_1:77592259-77592278 Msa0574240:intron 60.0%
AACAGCCCAAAGGACGCCGG+AGG - chr4_1:77591671-77591690 None:intergenic 65.0%
TCCGGCGTCCTTTGGGCTGT+TGG + chr4_1:77591670-77591689 Msa0574240:CDS 65.0%
CCCTCGCATGACCCGATAGC+TGG - chr4_1:77592261-77592280 None:intergenic 65.0%
AACAGCCCAAAGGACGCCGG+AGG - chr4_1:77591671-77591690 None:intergenic 65.0%
TCCGGCGTCCTTTGGGCTGT+TGG + chr4_1:77591670-77591689 Msa0574240:CDS 65.0%
CCCTCGCATGACCCGATAGC+TGG - chr4_1:77592261-77592280 None:intergenic 65.0%
GACGCCGGAGGTGGCGACTT+GGG - chr4_1:77591659-77591678 None:intergenic 70.0%
TCGCCACCTCCGGCGTCCTT+TGG + chr4_1:77591662-77591681 Msa0574240:CDS 70.0%
CGCCACCTCCGGCGTCCTTT+GGG + chr4_1:77591663-77591682 Msa0574240:CDS 70.0%
AGCCCAAAGGACGCCGGAGG+TGG - chr4_1:77591668-77591687 None:intergenic 70.0%
GACGCCGGAGGTGGCGACTT+GGG - chr4_1:77591659-77591678 None:intergenic 70.0%
TCGCCACCTCCGGCGTCCTT+TGG + chr4_1:77591662-77591681 Msa0574240:CDS 70.0%
CGCCACCTCCGGCGTCCTTT+GGG + chr4_1:77591663-77591682 Msa0574240:CDS 70.0%
AGCCCAAAGGACGCCGGAGG+TGG - chr4_1:77591668-77591687 None:intergenic 70.0%
GGACGCCGGAGGTGGCGACT+TGG - chr4_1:77591660-77591679 None:intergenic 75.0%
GGACGCCGGAGGTGGCGACT+TGG - chr4_1:77591660-77591679 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 77591335 77593157 77591335 ID=Msa0574240;Name=Msa0574240
chr4_1 mRNA 77591335 77593157 77591335 ID=Msa0574240-mRNA-1;Parent=Msa0574240;Name=Msa0574240-mRNA-1;_AED=0.06;_eAED=0.06;_QI=257|1|1|1|1|1|3|231|225
chr4_1 exon 77591335 77591768 77591335 ID=Msa0574240-mRNA-1:exon:27385;Parent=Msa0574240-mRNA-1
chr4_1 exon 77591864 77591961 77591864 ID=Msa0574240-mRNA-1:exon:27386;Parent=Msa0574240-mRNA-1
chr4_1 exon 77592524 77593157 77592524 ID=Msa0574240-mRNA-1:exon:27387;Parent=Msa0574240-mRNA-1
chr4_1 five_prime_UTR 77591335 77591591 77591335 ID=Msa0574240-mRNA-1:five_prime_utr;Parent=Msa0574240-mRNA-1
chr4_1 CDS 77591592 77591768 77591592 ID=Msa0574240-mRNA-1:cds;Parent=Msa0574240-mRNA-1
chr4_1 CDS 77591864 77591961 77591864 ID=Msa0574240-mRNA-1:cds;Parent=Msa0574240-mRNA-1
chr4_1 CDS 77592524 77592926 77592524 ID=Msa0574240-mRNA-1:cds;Parent=Msa0574240-mRNA-1
chr4_1 three_prime_UTR 77592927 77593157 77592927 ID=Msa0574240-mRNA-1:three_prime_utr;Parent=Msa0574240-mRNA-1
Gene Sequence

>Msa0574240

ATGTCGATGATATTGAAGGCATGGAATTGGTATCAGAATAGCCTCTCAATTCATCCAGTGAAGACCCAAGTCGCCACCTCCGGCGTCCTTTGGGCTGTTGGAGATGTCACTGCTCAGTACATCACTCACTCGGCAGCTGCAGCATCTTCTAAAAAGCGTCTTCAATTATCTGCCACCGAAGAAGCAACAGATGATAAATTTGTCATCGATTGGAGACGTGTTGCTGTTACTAGTATGTTTGGAATTGGTTTTGTTGGTCCAGTTGGCCATTTCTGGTATGAAGGCTTGGAAAAATTCATAAGTCACAAGCTTCATCTAATGCCACAAACTGCACGATCCGTAGCTACAAAAGTTGCAATGGACGGCTTGATTTTCGGTCCGGTGCATTTGTTTGTTTTCTTTACTTACATGGGATTGTCTGCTGGAAAGACTATTCCTGAGGTGAAGGAAGACTTGAAGAGGAATTATTTCCCAGCTTTGGTTTTAGAAGGTGGTGTGTGGCCAATTGTGCAGGTTTTTAATTTTCGGTATGTTCCTGTCAAGTACCAGCTTCTGTATGTAAACCTGTTCTGCTTGCTGGATAGTGCTTTCTTGTCTTGGTTGGAGCAACAGAAAGATGCAGCTTGGAAACAATGGTTTCAACCATTTCATTCTTCAAATGGAAAAGGTGGTCAATGA
Protein sequence

>Msa0574240

MSMILKAWNWYQNSLSIHPVKTQVATSGVLWAVGDVTAQYITHSAAAASSKKRLQLSATEEATDDKFVIDWRRVAVTSMFGIGFVGPVGHFWYEGLEKFISHKLHLMPQTARSVATKVAMDGLIFGPVHLFVFFTYMGLSAGKTIPEVKEDLKRNYFPALVLEGGVWPIVQVFNFRYVPVKYQLLYVNLFCLLDSAFLSWLEQQKDAAWKQWFQPFHSSNGKGGQ*