AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGGAACACATTCAATTT+TGG 0.182549 tig0046659:+377 Msa1466350:CDS
CTAAGAGAAGTACCGTTAAT+TGG 0.227037 tig0046659:-356 None:intergenic
CTTTGATGTGTAGTTCTTTC+GGG 0.236568 tig0046659:-470 None:intergenic
CTTCGTCGTCATCAGGTTCT+AGG 0.291225 tig0046659:+66 Msa1466350:CDS
GAACTCTAAATTGTGACTTT+TGG 0.313236 tig0046659:+800 Msa1466350:three_prime_UTR
CTCTATGGAGATTACTAAAT+AGG 0.315945 tig0046659:-511 None:intergenic
GCTTTGATGTGTAGTTCTTT+CGG 0.334131 tig0046659:-471 None:intergenic
ATCAAATGCGGGAACCTCTA+TGG 0.338170 tig0046659:-526 None:intergenic
AGTGGTGATGACTGGTTAAT+TGG 0.352807 tig0046659:+107 Msa1466350:CDS
TTAACGGTACTTCTCTTAGT+TGG 0.363400 tig0046659:+360 Msa1466350:CDS
GTTCATTGCAGAGGTAGTTA+TGG 0.424073 tig0046659:+283 Msa1466350:intron
AAATTGATTTAGTGTTTGTT+GGG 0.430422 tig0046659:+584 Msa1466350:three_prime_UTR
TGGTTGTCCACAACTAAAAC+AGG 0.430808 tig0046659:-311 None:intergenic
TAAGAGAAGTACCGTTAATT+GGG 0.442189 tig0046659:-355 None:intergenic
AAAATTGATTTAGTGTTTGT+TGG 0.443100 tig0046659:+583 Msa1466350:three_prime_UTR
TTTCTGAAAGACAGCTGAGC+AGG 0.451510 tig0046659:-653 None:intergenic
AACAGATCTTCGTCGTCATC+AGG 0.453544 tig0046659:+59 Msa1466350:CDS
TGTTTGAAAGAAAATTATAC+AGG 0.453994 tig0046659:-755 None:intergenic
CAATCTGACGCCCGATGCCC+TGG 0.458234 tig0046659:-331 None:intergenic
TTGTGGACAACCAGGGCATC+GGG 0.462191 tig0046659:+321 Msa1466350:CDS
TGAACCACTTAGGCCATCAC+TGG 0.484550 tig0046659:-429 None:intergenic
CGTCAGATTGTCCCAATTAA+CGG 0.488304 tig0046659:+344 Msa1466350:CDS
TAATATTTCTGTGGAGTGAA+TGG 0.497580 tig0046659:-711 None:intergenic
GTTGTGGACAACCAGGGCAT+CGG 0.502872 tig0046659:+320 Msa1466350:CDS
TTAAAACCAGTGGTGATGAC+TGG 0.517463 tig0046659:+99 Msa1466350:CDS
CTATTTAGTAATCTCCATAG+AGG 0.522599 tig0046659:+512 Msa1466350:three_prime_UTR
TTTGTTGGGGCAATGTTACT+CGG 0.528217 tig0046659:+598 Msa1466350:three_prime_UTR
TTCAATTTCTTGAACCACTT+AGG 0.535382 tig0046659:-439 None:intergenic
TTCGTCGTCATCAGGTTCTA+GGG 0.548645 tig0046659:+67 Msa1466350:CDS
CATTGCAGAGGTAGTTATGG+AGG 0.556291 tig0046659:+286 Msa1466350:intron
TACAAAAGCTAATATTTCTG+TGG 0.563248 tig0046659:-720 None:intergenic
AATTGATTTAGTGTTTGTTG+GGG 0.565006 tig0046659:+585 Msa1466350:three_prime_UTR
GGTCCATGTGCATAGTGAGC+AGG 0.569388 tig0046659:-831 None:intergenic
AATTAACCAGTCATCACCAC+TGG 0.576337 tig0046659:-105 None:intergenic
AAAAGTCTGATATCAAATGC+GGG 0.579088 tig0046659:-537 None:intergenic
AATTCAGCTGCATCCAGTGA+TGG 0.579381 tig0046659:+416 Msa1466350:CDS
GCATCCAGTGATGGCCTAAG+TGG 0.582998 tig0046659:+425 Msa1466350:CDS
ACTGGAGGTGGACGATCAAG+TGG 0.584590 tig0046659:+23 None:intergenic
ATTGCAGAGGTAGTTATGGA+GGG 0.595367 tig0046659:+287 Msa1466350:intron
ATACCTGCTCACTATGCACA+TGG 0.601321 tig0046659:+828 Msa1466350:three_prime_UTR
AAGTGGTTCAAGAAATTGAA+AGG 0.601500 tig0046659:+442 Msa1466350:exon
TCATCAGGTTCTAGGGAGAG+AGG 0.616285 tig0046659:+74 Msa1466350:CDS
CATACTTTGAAACAAACACA+TGG 0.626477 tig0046659:-852 None:intergenic
GGTGATGACTGGTTAATTGG+AGG 0.643911 tig0046659:+110 Msa1466350:CDS
TTGCAGAGGTAGTTATGGAG+GGG 0.644021 tig0046659:+288 Msa1466350:intron
CTTTCAAACAAGCTTTCCCG+TGG 0.646855 tig0046659:+768 Msa1466350:three_prime_UTR
TGCAGCTGAATTCTTGAACA+AGG 0.665556 tig0046659:-405 None:intergenic
CTGGAGGTGGACGATCAAGT+GGG 0.691338 tig0046659:+24 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATGCAAATATATTTATT+CGG + tig0046659:869-888 Msa1466350:three_prime_UTR 15.0%
!!! ACTAAATCAATTTTACATCA+CGG - tig0046659:577-596 None:intergenic 20.0%
!!! AAAATTGATTTAGTGTTTGT+TGG + tig0046659:583-602 Msa1466350:three_prime_UTR 20.0%
!!! AAATTGATTTAGTGTTTGTT+GGG + tig0046659:584-603 Msa1466350:three_prime_UTR 20.0%
!! TGTTTGAAAGAAAATTATAC+AGG - tig0046659:758-777 None:intergenic 20.0%
!!! ACTAAATCAATTTTACATCA+CGG - tig0046659:577-596 None:intergenic 20.0%
!!! AAAATTGATTTAGTGTTTGT+TGG + tig0046659:583-602 Msa1466350:three_prime_UTR 20.0%
!!! AAATTGATTTAGTGTTTGTT+GGG + tig0046659:584-603 Msa1466350:three_prime_UTR 20.0%
!! TGTTTGAAAGAAAATTATAC+AGG - tig0046659:758-777 None:intergenic 20.0%
! AGATTCGTTTGCTTTATATT+TGG + tig0046659:232-251 Msa1466350:intron 25.0%
! GATTCGTTTGCTTTATATTT+GGG + tig0046659:233-252 Msa1466350:intron 25.0%
! AAAAAGTCTGATATCAAATG+CGG - tig0046659:541-560 None:intergenic 25.0%
!! AATTGATTTAGTGTTTGTTG+GGG + tig0046659:585-604 Msa1466350:three_prime_UTR 25.0%
!! TACAAAAGCTAATATTTCTG+TGG - tig0046659:723-742 None:intergenic 25.0%
!!! ACAGAAATATTAGCTTTTGT+AGG + tig0046659:722-741 Msa1466350:three_prime_UTR 25.0%
! AGATTCGTTTGCTTTATATT+TGG + tig0046659:232-251 Msa1466350:intron 25.0%
! GATTCGTTTGCTTTATATTT+GGG + tig0046659:233-252 Msa1466350:intron 25.0%
! AAAAAGTCTGATATCAAATG+CGG - tig0046659:541-560 None:intergenic 25.0%
!! AATTGATTTAGTGTTTGTTG+GGG + tig0046659:585-604 Msa1466350:three_prime_UTR 25.0%
!! TACAAAAGCTAATATTTCTG+TGG - tig0046659:723-742 None:intergenic 25.0%
!!! ACAGAAATATTAGCTTTTGT+AGG + tig0046659:722-741 Msa1466350:three_prime_UTR 25.0%
!! TAAGAGAAGTACCGTTAATT+GGG - tig0046659:358-377 None:intergenic 30.0%
! AGTTGGAACACATTCAATTT+TGG + tig0046659:377-396 Msa1466350:CDS 30.0%
TTCAATTTCTTGAACCACTT+AGG - tig0046659:442-461 None:intergenic 30.0%
AAGTGGTTCAAGAAATTGAA+AGG + tig0046659:442-461 Msa1466350:exon 30.0%
CTCTATGGAGATTACTAAAT+AGG - tig0046659:514-533 None:intergenic 30.0%
CTATTTAGTAATCTCCATAG+AGG + tig0046659:512-531 Msa1466350:three_prime_UTR 30.0%
AAAAGTCTGATATCAAATGC+GGG - tig0046659:540-559 None:intergenic 30.0%
! TAATATTTCTGTGGAGTGAA+TGG - tig0046659:714-733 None:intergenic 30.0%
! GAACTCTAAATTGTGACTTT+TGG + tig0046659:800-819 Msa1466350:three_prime_UTR 30.0%
!! CATACTTTGAAACAAACACA+TGG - tig0046659:855-874 None:intergenic 30.0%
!! TAAGAGAAGTACCGTTAATT+GGG - tig0046659:358-377 None:intergenic 30.0%
! AGTTGGAACACATTCAATTT+TGG + tig0046659:377-396 Msa1466350:CDS 30.0%
TTCAATTTCTTGAACCACTT+AGG - tig0046659:442-461 None:intergenic 30.0%
AAGTGGTTCAAGAAATTGAA+AGG + tig0046659:442-461 Msa1466350:exon 30.0%
CTCTATGGAGATTACTAAAT+AGG - tig0046659:514-533 None:intergenic 30.0%
CTATTTAGTAATCTCCATAG+AGG + tig0046659:512-531 Msa1466350:three_prime_UTR 30.0%
AAAAGTCTGATATCAAATGC+GGG - tig0046659:540-559 None:intergenic 30.0%
! TAATATTTCTGTGGAGTGAA+TGG - tig0046659:714-733 None:intergenic 30.0%
! GAACTCTAAATTGTGACTTT+TGG + tig0046659:800-819 Msa1466350:three_prime_UTR 30.0%
!! CATACTTTGAAACAAACACA+TGG - tig0046659:855-874 None:intergenic 30.0%
ATTTCTTGTTACCTCCTGTT+TGG - tig0046659:179-198 None:intergenic 35.0%
!! CTAAGAGAAGTACCGTTAAT+TGG - tig0046659:359-378 None:intergenic 35.0%
TTAACGGTACTTCTCTTAGT+TGG + tig0046659:360-379 Msa1466350:CDS 35.0%
CTTTGATGTGTAGTTCTTTC+GGG - tig0046659:473-492 None:intergenic 35.0%
GCTTTGATGTGTAGTTCTTT+CGG - tig0046659:474-493 None:intergenic 35.0%
! TAGAGTTCATTTTCGTACCA+CGG - tig0046659:788-807 None:intergenic 35.0%
ATTTCTTGTTACCTCCTGTT+TGG - tig0046659:179-198 None:intergenic 35.0%
!! CTAAGAGAAGTACCGTTAAT+TGG - tig0046659:359-378 None:intergenic 35.0%
TTAACGGTACTTCTCTTAGT+TGG + tig0046659:360-379 Msa1466350:CDS 35.0%
CTTTGATGTGTAGTTCTTTC+GGG - tig0046659:473-492 None:intergenic 35.0%
GCTTTGATGTGTAGTTCTTT+CGG - tig0046659:474-493 None:intergenic 35.0%
! TAGAGTTCATTTTCGTACCA+CGG - tig0046659:788-807 None:intergenic 35.0%
!!! GAGAGAGGTTTTAAAACCAG+TGG + tig0046659:89-108 Msa1466350:CDS 40.0%
! TTAAAACCAGTGGTGATGAC+TGG + tig0046659:99-118 Msa1466350:CDS 40.0%
AATTAACCAGTCATCACCAC+TGG - tig0046659:108-127 None:intergenic 40.0%
! AGTGGTGATGACTGGTTAAT+TGG + tig0046659:107-126 Msa1466350:CDS 40.0%
ACAGATCTTCATCACCAAAC+AGG + tig0046659:162-181 Msa1466350:intron 40.0%
CATCTGAATGTTCATTGCAG+AGG + tig0046659:274-293 Msa1466350:intron 40.0%
GTTCATTGCAGAGGTAGTTA+TGG + tig0046659:283-302 Msa1466350:intron 40.0%
ATTGCAGAGGTAGTTATGGA+GGG + tig0046659:287-306 Msa1466350:intron 40.0%
TGGTTGTCCACAACTAAAAC+AGG - tig0046659:314-333 None:intergenic 40.0%
!!! TGTTTTAGTTGTGGACAACC+AGG + tig0046659:313-332 Msa1466350:CDS 40.0%
!!! GTTTTAGTTGTGGACAACCA+GGG + tig0046659:314-333 Msa1466350:CDS 40.0%
CGTCAGATTGTCCCAATTAA+CGG + tig0046659:344-363 Msa1466350:CDS 40.0%
TGCAGCTGAATTCTTGAACA+AGG - tig0046659:408-427 None:intergenic 40.0%
TTTGTTGGGGCAATGTTACT+CGG + tig0046659:598-617 Msa1466350:three_prime_UTR 40.0%
GCTGTCTTTCAGAAAAAGAC+AGG + tig0046659:661-680 Msa1466350:three_prime_UTR 40.0%
! AGAGTTCATTTTCGTACCAC+GGG - tig0046659:787-806 None:intergenic 40.0%
!!! GAGAGAGGTTTTAAAACCAG+TGG + tig0046659:89-108 Msa1466350:CDS 40.0%
! TTAAAACCAGTGGTGATGAC+TGG + tig0046659:99-118 Msa1466350:CDS 40.0%
AATTAACCAGTCATCACCAC+TGG - tig0046659:108-127 None:intergenic 40.0%
! AGTGGTGATGACTGGTTAAT+TGG + tig0046659:107-126 Msa1466350:CDS 40.0%
ACAGATCTTCATCACCAAAC+AGG + tig0046659:162-181 Msa1466350:intron 40.0%
CATCTGAATGTTCATTGCAG+AGG + tig0046659:274-293 Msa1466350:intron 40.0%
GTTCATTGCAGAGGTAGTTA+TGG + tig0046659:283-302 Msa1466350:intron 40.0%
ATTGCAGAGGTAGTTATGGA+GGG + tig0046659:287-306 Msa1466350:intron 40.0%
TGGTTGTCCACAACTAAAAC+AGG - tig0046659:314-333 None:intergenic 40.0%
!!! TGTTTTAGTTGTGGACAACC+AGG + tig0046659:313-332 Msa1466350:CDS 40.0%
!!! GTTTTAGTTGTGGACAACCA+GGG + tig0046659:314-333 Msa1466350:CDS 40.0%
CGTCAGATTGTCCCAATTAA+CGG + tig0046659:344-363 Msa1466350:CDS 40.0%
TGCAGCTGAATTCTTGAACA+AGG - tig0046659:408-427 None:intergenic 40.0%
TTTGTTGGGGCAATGTTACT+CGG + tig0046659:598-617 Msa1466350:three_prime_UTR 40.0%
GCTGTCTTTCAGAAAAAGAC+AGG + tig0046659:661-680 Msa1466350:three_prime_UTR 40.0%
! AGAGTTCATTTTCGTACCAC+GGG - tig0046659:787-806 None:intergenic 40.0%
AACAGATCTTCGTCGTCATC+AGG + tig0046659:59-78 Msa1466350:CDS 45.0%
TTCGTCGTCATCAGGTTCTA+GGG + tig0046659:67-86 Msa1466350:CDS 45.0%
! GGTGATGACTGGTTAATTGG+AGG + tig0046659:110-129 Msa1466350:CDS 45.0%
ATTGGAGGTAGACGATCAAG+CGG + tig0046659:125-144 Msa1466350:intron 45.0%
GATCTTCATCACCAAACAGG+AGG + tig0046659:165-184 Msa1466350:intron 45.0%
TTGGGTGTGCGTATAACTCA+TGG + tig0046659:251-270 Msa1466350:intron 45.0%
CATTGCAGAGGTAGTTATGG+AGG + tig0046659:286-305 Msa1466350:intron 45.0%
TTGCAGAGGTAGTTATGGAG+GGG + tig0046659:288-307 Msa1466350:intron 45.0%
AATTCAGCTGCATCCAGTGA+TGG + tig0046659:416-435 Msa1466350:CDS 45.0%
ATCAAATGCGGGAACCTCTA+TGG - tig0046659:529-548 None:intergenic 45.0%
! TTTCTGAAAGACAGCTGAGC+AGG - tig0046659:656-675 None:intergenic 45.0%
CTTTCAAACAAGCTTTCCCG+TGG + tig0046659:768-787 Msa1466350:three_prime_UTR 45.0%
ATACCTGCTCACTATGCACA+TGG + tig0046659:828-847 Msa1466350:three_prime_UTR 45.0%
AACAGATCTTCGTCGTCATC+AGG + tig0046659:59-78 Msa1466350:CDS 45.0%
TTCGTCGTCATCAGGTTCTA+GGG + tig0046659:67-86 Msa1466350:CDS 45.0%
! GGTGATGACTGGTTAATTGG+AGG + tig0046659:110-129 Msa1466350:CDS 45.0%
ATTGGAGGTAGACGATCAAG+CGG + tig0046659:125-144 Msa1466350:intron 45.0%
GATCTTCATCACCAAACAGG+AGG + tig0046659:165-184 Msa1466350:intron 45.0%
TTGGGTGTGCGTATAACTCA+TGG + tig0046659:251-270 Msa1466350:intron 45.0%
CATTGCAGAGGTAGTTATGG+AGG + tig0046659:286-305 Msa1466350:intron 45.0%
TTGCAGAGGTAGTTATGGAG+GGG + tig0046659:288-307 Msa1466350:intron 45.0%
AATTCAGCTGCATCCAGTGA+TGG + tig0046659:416-435 Msa1466350:CDS 45.0%
ATCAAATGCGGGAACCTCTA+TGG - tig0046659:529-548 None:intergenic 45.0%
! TTTCTGAAAGACAGCTGAGC+AGG - tig0046659:656-675 None:intergenic 45.0%
CTTTCAAACAAGCTTTCCCG+TGG + tig0046659:768-787 Msa1466350:three_prime_UTR 45.0%
ATACCTGCTCACTATGCACA+TGG + tig0046659:828-847 Msa1466350:three_prime_UTR 45.0%
CTTCGTCGTCATCAGGTTCT+AGG + tig0046659:66-85 Msa1466350:CDS 50.0%
!! TCATCAGGTTCTAGGGAGAG+AGG + tig0046659:74-93 Msa1466350:CDS 50.0%
TTGGAGGTAGACGATCAAGC+GGG + tig0046659:126-145 Msa1466350:intron 50.0%
TGAACCACTTAGGCCATCAC+TGG - tig0046659:432-451 None:intergenic 50.0%
CTTCGTCGTCATCAGGTTCT+AGG + tig0046659:66-85 Msa1466350:CDS 50.0%
!! TCATCAGGTTCTAGGGAGAG+AGG + tig0046659:74-93 Msa1466350:CDS 50.0%
TTGGAGGTAGACGATCAAGC+GGG + tig0046659:126-145 Msa1466350:intron 50.0%
TGAACCACTTAGGCCATCAC+TGG - tig0046659:432-451 None:intergenic 50.0%
!!! GGAGGGGCCTGTTTTAGTTG+TGG + tig0046659:304-323 Msa1466350:CDS 55.0%
GTTGTGGACAACCAGGGCAT+CGG + tig0046659:320-339 Msa1466350:CDS 55.0%
! TTGTGGACAACCAGGGCATC+GGG + tig0046659:321-340 Msa1466350:CDS 55.0%
GCATCCAGTGATGGCCTAAG+TGG + tig0046659:425-444 Msa1466350:CDS 55.0%
GGTCCATGTGCATAGTGAGC+AGG - tig0046659:834-853 None:intergenic 55.0%
!!! GGAGGGGCCTGTTTTAGTTG+TGG + tig0046659:304-323 Msa1466350:CDS 55.0%
GTTGTGGACAACCAGGGCAT+CGG + tig0046659:320-339 Msa1466350:CDS 55.0%
! TTGTGGACAACCAGGGCATC+GGG + tig0046659:321-340 Msa1466350:CDS 55.0%
GCATCCAGTGATGGCCTAAG+TGG + tig0046659:425-444 Msa1466350:CDS 55.0%
GGTCCATGTGCATAGTGAGC+AGG - tig0046659:834-853 None:intergenic 55.0%
CAATCTGACGCCCGATGCCC+TGG - tig0046659:334-353 None:intergenic 65.0%
CAATCTGACGCCCGATGCCC+TGG - tig0046659:334-353 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0046659 gene 32 889 32 ID=Msa1466350;Name=Msa1466350
tig0046659 mRNA 32 889 32 ID=Msa1466350-mRNA-1;Parent=Msa1466350;Name=Msa1466350-mRNA-1;_AED=0.05;_eAED=0.19;_QI=0|0|0.5|1|1|0.5|2|429|88
tig0046659 exon 32 131 32 ID=Msa1466350-mRNA-1:exon:25057;Parent=Msa1466350-mRNA-1
tig0046659 exon 294 889 294 ID=Msa1466350-mRNA-1:exon:25058;Parent=Msa1466350-mRNA-1
tig0046659 CDS 32 131 32 ID=Msa1466350-mRNA-1:cds;Parent=Msa1466350-mRNA-1
tig0046659 CDS 294 460 294 ID=Msa1466350-mRNA-1:cds;Parent=Msa1466350-mRNA-1
tig0046659 three_prime_UTR 461 889 461 ID=Msa1466350-mRNA-1:three_prime_utr;Parent=Msa1466350-mRNA-1
Gene Sequence

>Msa1466350

GGACGATCAAGTGGGTATTCATCATCAAACAGATCTTCGTCGTCATCAGGTTCTAGGGAGAGAGGTTTTAAAACCAGTGGTGATGACTGGTTAATTGGAGAGGTAGTTATGGAGGGGCCTGTTTTAGTTGTGGACAACCAGGGCATCGGGCGTCAGATTGTCCCAATTAACGGTACTTCTCTTAGTTGGAACACATTCAATTTTGGCGTCACCTTGTTCAAGAATTCAGCTGCATCCAGTGATGGCCTAAGTGGTTCAAGAAATTGA

Protein sequence

>Msa1466350

GRSSGYSSSNRSSSSSGSRERGFKTSGDDWLIGEVVMEGPVLVVDNQGIGRQIVPINGTSLSWNTFNFGVTLFKNSAASSDGLSGSRN*