Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa0592680 | 0.815268 | 1.008479e-51 | -8.615850e-47 |
| Msa0067140 | Msa0592680 | 0.812985 | 3.218477e-51 | -8.615850e-47 |
| Msa0110270 | Msa0592680 | 0.800243 | 1.579121e-48 | -8.615850e-47 |
| Msa0131770 | Msa0592680 | 0.811926 | 5.484143e-51 | -8.615850e-47 |
| Msa0142220 | Msa0592680 | 0.822763 | 1.990950e-53 | -8.615850e-47 |
| Msa0153910 | Msa0592680 | 0.823980 | 1.034223e-53 | -8.615850e-47 |
| Msa0394970 | Msa0592680 | 0.801849 | 7.416260e-49 | -8.615850e-47 |
| Msa0402290 | Msa0592680 | 0.808532 | 2.957642e-50 | -8.615850e-47 |
| Msa0436780 | Msa0592680 | 0.801665 | 8.087111e-49 | -8.615850e-47 |
| Msa0270520 | Msa0592680 | 0.805369 | 1.379751e-49 | -8.615850e-47 |
| Msa0273100 | Msa0592680 | 0.809733 | 1.635110e-50 | -8.615850e-47 |
| Msa0309020 | Msa0592680 | 0.818979 | 1.477548e-52 | -8.615850e-47 |
| Msa0592680 | Msa0600700 | 0.820908 | 5.347590e-53 | -8.615850e-47 |
| Msa0592680 | Msa0678930 | 0.809371 | 1.956311e-50 | -8.615850e-47 |
| Msa0592680 | Msa0694370 | 0.805117 | 1.558303e-49 | -8.615850e-47 |
| Msa0592680 | Msa0735170 | 0.801945 | 7.087799e-49 | -8.615850e-47 |
| Msa0592680 | Msa0741990 | 0.815451 | 9.182779e-52 | -8.615850e-47 |
| Msa0592680 | Msa0760130 | 0.801159 | 1.026723e-48 | -8.615850e-47 |
| Msa0592680 | Msa0766630 | 0.816797 | 4.593839e-52 | -8.615850e-47 |
| Msa0592680 | Msa0822650 | 0.810248 | 1.266713e-50 | -8.615850e-47 |
| Msa0592680 | Msa0885280 | 0.813557 | 2.410140e-51 | -8.615850e-47 |
| Msa0592680 | Msa0885290 | 0.834090 | 3.665033e-56 | -8.615850e-47 |
| Msa0592680 | Msa0935910 | 0.822642 | 2.123984e-53 | -8.615850e-47 |
| Msa0592680 | Msa0954910 | 0.804208 | 2.412095e-49 | -8.615850e-47 |
| Msa0592680 | Msa0969450 | 0.809436 | 1.893718e-50 | -8.615850e-47 |
| Msa0592680 | Msa0969520 | 0.805285 | 1.436814e-49 | -8.615850e-47 |
| Msa0592680 | Msa1014540 | 0.812451 | 4.211705e-51 | -8.615850e-47 |
| Msa0592680 | Msa1121480 | 0.804151 | 2.478689e-49 | -8.615850e-47 |
| Msa0592680 | Msa1121490 | 0.811514 | 6.740446e-51 | -8.615850e-47 |
| Msa0592680 | Msa1160670 | 0.808895 | 2.474058e-50 | -8.615850e-47 |
| Msa0592680 | Msa1187950 | 0.804096 | 2.544654e-49 | -8.615850e-47 |
| Msa0592680 | Msa1285930 | 0.800598 | 1.336794e-48 | -8.615850e-47 |
| Msa0592680 | Msa1438250 | 0.823723 | 1.188215e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCTAAATAATGTATCTTT+TGG | 0.234825 | 4_2:+18192316 | None:intergenic |
| CTTAACTAACCATTTGTATC+AGG | 0.282721 | 4_2:+18192641 | None:intergenic |
| CCTTTATCTGCCTTCTAATA+CGG | 0.357251 | 4_2:+18192456 | None:intergenic |
| CCGTATTAGAAGGCAGATAA+AGG | 0.375867 | 4_2:-18192456 | Msa0592680:CDS |
| AAGGGACAAACCGTATTAGA+AGG | 0.460786 | 4_2:-18192466 | Msa0592680:CDS |
| GCGAGGTTCACTGCACTCTC+TGG | 0.485362 | 4_2:-18192373 | Msa0592680:CDS |
| TCACTGGAAAGGTAAGGATT+AGG | 0.493319 | 4_2:-18192418 | Msa0592680:CDS |
| AGCTCTAAACCTGATACAAA+TGG | 0.520906 | 4_2:-18192650 | Msa0592680:intron |
| GTATGATCACTGGAAAGGTA+AGG | 0.565880 | 4_2:-18192424 | Msa0592680:CDS |
| AAATAGTATGATCACTGGAA+AGG | 0.576598 | 4_2:-18192429 | Msa0592680:CDS |
| AATTAGTTCATGTAGAAGAA+GGG | 0.608333 | 4_2:-18192484 | Msa0592680:intron |
| AGCCAATAAAACTTACAGCG+AGG | 0.697440 | 4_2:-18192390 | Msa0592680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAGAAAAATAAACATA+TGG | + | chr4_2:18192456-18192475 | None:intergenic | 15.0% |
| ! | AACTAATTATCAGAAGCAAA+GGG | + | chr4_2:18192485-18192504 | None:intergenic | 25.0% |
| ! | TAATTAGTTCATGTAGAAGA+AGG | - | chr4_2:18192496-18192515 | Msa0592680:intron | 25.0% |
| ! | AATTAGTTCATGTAGAAGAA+GGG | - | chr4_2:18192497-18192516 | Msa0592680:intron | 25.0% |
| ! | GTTAAAAATAGTATGATCAC+TGG | - | chr4_2:18192547-18192566 | Msa0592680:intron | 25.0% |
| !! | GCTAACATTTTTCATATCTA+TGG | - | chr4_2:18192630-18192649 | Msa0592680:intron | 25.0% |
| !!! | TAATGTATCTTTTGGAGTAA+AGG | + | chr4_2:18192660-18192679 | None:intergenic | 25.0% |
| CTTAACTAACCATTTGTATC+AGG | + | chr4_2:18192343-18192362 | None:intergenic | 30.0% | |
| TGGTAGTTATAACGTTATTG+TGG | + | chr4_2:18192436-18192455 | None:intergenic | 30.0% | |
| GAACTAATTATCAGAAGCAA+AGG | + | chr4_2:18192486-18192505 | None:intergenic | 30.0% | |
| AAATAGTATGATCACTGGAA+AGG | - | chr4_2:18192552-18192571 | Msa0592680:intron | 30.0% | |
| !! | AGCTCTAAACCTGATACAAA+TGG | - | chr4_2:18192331-18192350 | Msa0592680:CDS | 35.0% |
| !! | AGAAGACTAAGTTGATCACT+TGG | + | chr4_2:18192407-18192426 | None:intergenic | 35.0% |
| CCTTTATCTGCCTTCTAATA+CGG | + | chr4_2:18192528-18192547 | None:intergenic | 35.0% | |
| !! | AACCTCGCTGTAAGTTTTAT+TGG | + | chr4_2:18192596-18192615 | None:intergenic | 35.0% |
| !!! | GTATCTTTTGGAGTAAAGGT+TGG | + | chr4_2:18192656-18192675 | None:intergenic | 35.0% |
| AAGGGACAAACCGTATTAGA+AGG | - | chr4_2:18192515-18192534 | Msa0592680:intron | 40.0% | |
| CCGTATTAGAAGGCAGATAA+AGG | - | chr4_2:18192525-18192544 | Msa0592680:intron | 40.0% | |
| GTATGATCACTGGAAAGGTA+AGG | - | chr4_2:18192557-18192576 | Msa0592680:intron | 40.0% | |
| TCACTGGAAAGGTAAGGATT+AGG | - | chr4_2:18192563-18192582 | Msa0592680:intron | 40.0% | |
| AGCCAATAAAACTTACAGCG+AGG | - | chr4_2:18192591-18192610 | Msa0592680:intron | 40.0% | |
| GCGAGGTTCACTGCACTCTC+TGG | - | chr4_2:18192608-18192627 | Msa0592680:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 18192319 | 18192684 | 18192319 | ID=Msa0592680;Name=Msa0592680 |
| chr4_2 | mRNA | 18192319 | 18192684 | 18192319 | ID=Msa0592680-mRNA-1;Parent=Msa0592680;Name=Msa0592680-mRNA-1;_AED=0.79;_eAED=1.00;_QI=0|0|0|0.5|0|0.5|2|0|68 |
| chr4_2 | exon | 18192651 | 18192684 | 18192651 | ID=Msa0592680-mRNA-1:exon:5317;Parent=Msa0592680-mRNA-1 |
| chr4_2 | exon | 18192319 | 18192491 | 18192319 | ID=Msa0592680-mRNA-1:exon:5316;Parent=Msa0592680-mRNA-1 |
| chr4_2 | CDS | 18192651 | 18192684 | 18192651 | ID=Msa0592680-mRNA-1:cds;Parent=Msa0592680-mRNA-1 |
| chr4_2 | CDS | 18192319 | 18192491 | 18192319 | ID=Msa0592680-mRNA-1:cds;Parent=Msa0592680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
