AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0667030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0667030 MtrunA17_Chr4g0005561 100.000 170 0 0 1 170 1 170 4.65e-124 346
Msa0667030 MtrunA17_Chr7g0243261 84.706 170 26 0 1 170 1 170 1.51e-104 296
Msa0667030 MtrunA17_Chr5g0418311 51.205 166 78 1 8 170 15 180 1.51e-59 183
Msa0667030 MtrunA17_Chr4g0061181 46.552 174 89 1 1 170 1 174 1.47e-55 172
Msa0667030 MtrunA17_Chr7g0269431 41.772 79 44 2 56 133 33 110 7.92e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0667030 AT3G08890.1 77.647 170 38 0 1 170 1 170 8.42e-100 285
Msa0667030 AT3G08890.2 77.647 170 38 0 1 170 1 170 8.42e-100 285
Msa0667030 AT5G37070.1 78.824 170 36 0 1 170 1 170 1.06e-98 282
Msa0667030 AT5G01610.2 75.882 170 41 0 1 170 1 170 2.23e-96 276
Msa0667030 AT5G01610.1 75.882 170 41 0 1 170 1 170 2.23e-96 276
Msa0667030 AT2G03350.1 50.000 164 79 1 7 167 12 175 2.94e-53 167
Msa0667030 AT1G02816.1 25.217 115 83 2 57 168 35 149 1.54e-12 62.8
Msa0667030 AT4G02370.1 27.368 95 68 1 56 149 34 128 1.39e-11 60.1
Msa0667030 AT4G02360.1 29.358 109 74 3 51 157 25 132 2.03e-11 59.3

Find 69 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTCTTCACCACTGTTAC+TGG 0.210400 4_4:+7257632 Msa0667030:CDS
TCATATGATATATTGTTATT+AGG 0.213758 4_4:+7257844 Msa0667030:three_prime_UTR
ATCAAGTAACTGTTGCTATT+TGG 0.237961 4_4:+7258039 Msa0667030:three_prime_UTR
CACCACTGTTACTGGTTATC+TGG 0.249379 4_4:+7257640 Msa0667030:CDS
TGAAGACAAAAGTTATTATC+TGG 0.261455 4_4:+7257693 Msa0667030:CDS
CTTCGATCCCTCGGAGAAAA+TGG 0.294455 4_4:-7257729 None:intergenic
GCCATTACCAGAGTTGTTAA+AGG 0.295014 4_4:+7257481 Msa0667030:CDS
TGGTTGGTCAACAAAGTAAA+AGG 0.300821 4_4:+7256215 Msa0667030:CDS
ATCAAGGATGTTCATGGTTC+GGG 0.327372 4_4:-7258133 None:intergenic
TACTTTGTTGACCAACCATT+TGG 0.339849 4_4:-7256210 None:intergenic
AGTACTGATCCTTGGACCTT+GGG 0.351627 4_4:+7257968 Msa0667030:three_prime_UTR
TTTAGTATCAAGGATGTTCA+TGG 0.360314 4_4:-7258139 None:intergenic
CGTGATACTCAACACGGTTT+GGG 0.367490 4_4:-7258100 None:intergenic
ACGTGATACTCAACACGGTT+TGG 0.373063 4_4:-7258101 None:intergenic
GACAGAATTAAGCTCCTTGC+TGG 0.374345 4_4:-7255058 None:intergenic
TACTCAACACGGTTTGGGTT+TGG 0.388586 4_4:-7258095 None:intergenic
TATCAAGGATGTTCATGGTT+CGG 0.388822 4_4:-7258134 None:intergenic
ATTGTAGAAAGAAGGAGAAA+TGG 0.390997 4_4:+7254990 Msa0667030:exon
GGATGTTCATGGTTCGGGTT+CGG 0.401982 4_4:-7258128 None:intergenic
TCAACAAGGTTGGATCCTAT+TGG 0.402846 4_4:+7255022 Msa0667030:CDS
TCCTTTAACAACTCTGGTAA+TGG 0.418016 4_4:-7257482 None:intergenic
AAGTACTGATCCTTGGACCT+TGG 0.424944 4_4:+7257967 Msa0667030:three_prime_UTR
GGCTTTCTGATTGAACCAAT+AGG 0.431334 4_4:-7255037 None:intergenic
GGTCTGCGAAGTAGGCTATA+AGG 0.432562 4_4:+7257595 Msa0667030:CDS
TTGAGTCTTCTTGTATGGTT+GGG 0.436411 4_4:+7257910 Msa0667030:three_prime_UTR
CCATACAAGAAGACTCAAAA+TGG 0.436737 4_4:-7257905 None:intergenic
TTTGAGTCTTCTTGTATGGT+TGG 0.437027 4_4:+7257909 Msa0667030:three_prime_UTR
AAGGAGTATGATCTACCAAT+AGG 0.443273 4_4:+7257500 Msa0667030:CDS
TCTTTCTGATTGTAGAAAGA+AGG 0.444898 4_4:+7254982 Msa0667030:five_prime_UTR
ATTACTACTCTTTAGTATCA+AGG 0.447460 4_4:-7258149 None:intergenic
AGGAAAGCTAGCAGACATAG+AGG 0.449690 4_4:+7257667 Msa0667030:CDS
ATTCCTCAGGTCTGCGAAGT+AGG 0.457321 4_4:+7257587 Msa0667030:CDS
GTATTTCAAGTACTGATCCT+TGG 0.461000 4_4:+7257960 Msa0667030:three_prime_UTR
CACATAGAGCTTCGATCCCT+CGG 0.465720 4_4:-7257738 None:intergenic
TCACGAGGAAAGATTCCTAT+TGG 0.469668 4_4:-7257515 None:intergenic
AAAACAGAAAACAGGGATGA+TGG 0.476992 4_4:-7254948 None:intergenic
GCTTGAAGTCTTCATTCCTC+AGG 0.483186 4_4:+7257574 Msa0667030:CDS
GATCAAGTATTCAACAAGGT+TGG 0.497292 4_4:+7255012 Msa0667030:CDS
GAAGACAAAAGTTATTATCT+GGG 0.508796 4_4:+7257694 Msa0667030:CDS
AAGTTGGCTGCTTATTCGCT+AGG 0.523175 4_4:-7254911 None:intergenic
GCATATGATGTTACAAGAGA+TGG 0.523208 4_4:+7257794 Msa0667030:CDS
TCGAAGCTCTATGTGACTGC+TGG 0.534882 4_4:+7257746 Msa0667030:CDS
GTATCGAAGGCGGAACCAAA+TGG 0.539035 4_4:+7256195 Msa0667030:CDS
TAGCGAATAAGCAGCCAACT+TGG 0.539393 4_4:+7254913 Msa0667030:five_prime_UTR
GTTACTGGTTATCTGGAGAA+AGG 0.540770 4_4:+7257647 Msa0667030:CDS
GCATGAAGAAAACAAGGAGT+AGG 0.544902 4_4:+7257768 Msa0667030:CDS
TCATACTCCTTTAACAACTC+TGG 0.548889 4_4:-7257488 None:intergenic
AGTCTTCTTGTATGGTTGGG+TGG 0.550498 4_4:+7257913 Msa0667030:three_prime_UTR
CTGCTGGCATGAAGAAAACA+AGG 0.561931 4_4:+7257762 Msa0667030:CDS
TACGAATTTAACGAAGAGAC+GGG 0.578620 4_4:+7257548 Msa0667030:CDS
CTACGAATTTAACGAAGAGA+CGG 0.579413 4_4:+7257547 Msa0667030:CDS
TCGTAGTTTGTTGCATCACG+AGG 0.584895 4_4:-7257530 None:intergenic
AAATCACAACCCAAGGTCCA+AGG 0.587281 4_4:-7257977 None:intergenic
AGCAAGGAGCTTAATTCTGT+CGG 0.592969 4_4:+7255060 Msa0667030:CDS
CATGAAGAAAACAAGGAGTA+GGG 0.600568 4_4:+7257769 Msa0667030:CDS
GGAAAGCTAGCAGACATAGA+GGG 0.604204 4_4:+7257668 Msa0667030:CDS
CGAAGGCGGAACCAAATGGT+TGG 0.621013 4_4:+7256199 Msa0667030:CDS
GTTCAATCAGAAAGCCAGCA+AGG 0.622010 4_4:+7255044 Msa0667030:CDS
GGACAACAACGATACCAAGT+TGG 0.626792 4_4:-7254927 None:intergenic
ATTTCTCAAATCACAACCCA+AGG 0.635629 4_4:-7257984 None:intergenic
GGGTTGTGATTTGAGAAATG+TGG 0.644526 4_4:+7257988 Msa0667030:three_prime_UTR
TCACTGTCGAACAGTATCGA+AGG 0.655695 4_4:+7256182 Msa0667030:CDS
TAGCCTACTTCGCAGACCTG+AGG 0.660590 4_4:-7257590 None:intergenic
CTCCAGATAACCAGTAACAG+TGG 0.669904 4_4:-7257642 None:intergenic
GGTCTATAAAGAAACAACAA+AGG 0.670169 4_4:-7257884 None:intergenic
AGTGGTGAAGAAACGCAAGA+CGG 0.697268 4_4:-7257624 None:intergenic
AATGGATCAAGTATTCAACA+AGG 0.730089 4_4:+7255008 Msa0667030:exon
GCTAAACGTGATACTCAACA+CGG 0.730728 4_4:-7258106 None:intergenic
CTGTCGAACAGTATCGAAGG+CGG 0.747673 4_4:+7256185 Msa0667030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAAATTATTAATTTT+TGG + chr4_4:7255156-7255175 Msa0667030:intron 10.0%
!!! TATCCAAAAATTAATAATTT+TGG - chr4_4:7255162-7255181 None:intergenic 10.0%
!! AAAAACTGAAAAATTGAAAA+GGG - chr4_4:7255114-7255133 None:intergenic 15.0%
!! AAAAAACTGAAAAATTGAAA+AGG - chr4_4:7255115-7255134 None:intergenic 15.0%
!! AAAATAATTTAACAGTGAAA+AGG + chr4_4:7256972-7256991 Msa0667030:intron 15.0%
!! TCTATTAACAAAATTATAGA+AGG - chr4_4:7257103-7257122 None:intergenic 15.0%
!!! TCATATGATATATTGTTATT+AGG + chr4_4:7257844-7257863 Msa0667030:three_prime_UTR 15.0%
!! AAAACTGAAAAATTGAAAAG+GGG - chr4_4:7255113-7255132 None:intergenic 20.0%
!! ATTGAATTGAATTGAATTGA+TGG + chr4_4:7255226-7255245 Msa0667030:intron 20.0%
!! TTGAATTGAATTGAATTGAT+GGG + chr4_4:7255227-7255246 Msa0667030:intron 20.0%
!!! AAGATATCTGAATTTTGAAT+TGG + chr4_4:7255278-7255297 Msa0667030:intron 20.0%
!! TATTTAATTCTGATTAAGCA+AGG + chr4_4:7255935-7255954 Msa0667030:intron 20.0%
!! ATTTAATTCTGATTAAGCAA+GGG + chr4_4:7255936-7255955 Msa0667030:intron 20.0%
!! TTCTATCAATTTCTCAATTT+GGG - chr4_4:7256352-7256371 None:intergenic 20.0%
!! TTTCTATCAATTTCTCAATT+TGG - chr4_4:7256353-7256372 None:intergenic 20.0%
!!! TCACTTATAAATACATTTTC+AGG - chr4_4:7256586-7256605 None:intergenic 20.0%
!! TTAATATACACCTTAGTAAT+TGG + chr4_4:7256729-7256748 Msa0667030:intron 20.0%
!! AAATACATAAGTGTATTTGT+TGG + chr4_4:7257318-7257337 Msa0667030:intron 20.0%
!! AGAATAGATTTCTATATTGA+AGG + chr4_4:7257421-7257440 Msa0667030:intron 20.0%
!! GAATAGATTTCTATATTGAA+GGG + chr4_4:7257422-7257441 Msa0667030:intron 20.0%
! ATGAACAAAAACAGAAAACA+GGG - chr4_4:7254958-7254977 None:intergenic 25.0%
! AATGAACAAAAACAGAAAAC+AGG - chr4_4:7254959-7254978 None:intergenic 25.0%
!! TTTTGGATAATTAAACCTCA+TGG + chr4_4:7255173-7255192 Msa0667030:intron 25.0%
! TAATTAAACCTCATGGAAAT+TGG + chr4_4:7255180-7255199 Msa0667030:intron 25.0%
! AACAAACATAAACATCATTG+TGG - chr4_4:7255316-7255335 None:intergenic 25.0%
! ACAATGATGTTTATGTTTGT+TGG + chr4_4:7255315-7255334 Msa0667030:intron 25.0%
!!! TTTTAGGTGATTTTAAGTAC+TGG + chr4_4:7255634-7255653 Msa0667030:intron 25.0%
! TTAATGAGATGATTCTGTTA+CGG + chr4_4:7255825-7255844 Msa0667030:intron 25.0%
! CATGTCAGTAAACATAATTT+AGG + chr4_4:7255885-7255904 Msa0667030:intron 25.0%
!! AAAGATGAATTTTCATTGCT+TGG + chr4_4:7256120-7256139 Msa0667030:intron 25.0%
! ATAAATGTTTGAGCAACATA+TGG - chr4_4:7256257-7256276 None:intergenic 25.0%
! TTGAGAAATTGATAGAAAAC+TGG + chr4_4:7256355-7256374 Msa0667030:intron 25.0%
!! AATTTACAAGCCTATTTTGT+AGG + chr4_4:7256618-7256637 Msa0667030:intron 25.0%
!! ATTTACAAGCCTATTTTGTA+GGG + chr4_4:7256619-7256638 Msa0667030:intron 25.0%
! TTGGAAGGAAAAATTGAATT+GGG - chr4_4:7256655-7256674 None:intergenic 25.0%
! ATTGGAAGGAAAAATTGAAT+TGG - chr4_4:7256656-7256675 None:intergenic 25.0%
! TTGATGTAATTACATGCAAT+GGG - chr4_4:7256680-7256699 None:intergenic 25.0%
! AAATTTAGTACTTTGAACCA+TGG + chr4_4:7256828-7256847 Msa0667030:intron 25.0%
!! GGTCTGTTTATAAGTTATTT+TGG + chr4_4:7256852-7256871 Msa0667030:intron 25.0%
!! CAAAAGACTTCTAAAAATTG+AGG - chr4_4:7257058-7257077 None:intergenic 25.0%
!!! CAATTTTTAGAAGTCTTTTG+AGG + chr4_4:7257058-7257077 Msa0667030:intron 25.0%
! ATAATAGACAATTCATGAAG+TGG - chr4_4:7257192-7257211 None:intergenic 25.0%
! TGAATTGTCTATTATCCAAA+AGG + chr4_4:7257197-7257216 Msa0667030:intron 25.0%
! AATAAGTATGAACATCATGA+AGG + chr4_4:7257232-7257251 Msa0667030:intron 25.0%
! ATAAGTATGAACATCATGAA+GGG + chr4_4:7257233-7257252 Msa0667030:intron 25.0%
!!! TATCTCTAAAACTGTAGTAA+TGG - chr4_4:7257297-7257316 None:intergenic 25.0%
! TCTATTTGACATAGCAATAA+TGG + chr4_4:7257382-7257401 Msa0667030:intron 25.0%
! ACTTAATTATATCACTTTGC+AGG + chr4_4:7257446-7257465 Msa0667030:intron 25.0%
! TGAAGACAAAAGTTATTATC+TGG + chr4_4:7257693-7257712 Msa0667030:CDS 25.0%
! GAAGACAAAAGTTATTATCT+GGG + chr4_4:7257694-7257713 Msa0667030:CDS 25.0%
!!! AAGTTTTAGAACTTATCTAC+AGG - chr4_4:7257824-7257843 None:intergenic 25.0%
! ATTACTACTCTTTAGTATCA+AGG - chr4_4:7258152-7258171 None:intergenic 25.0%
TCTTTCTGATTGTAGAAAGA+AGG + chr4_4:7254982-7255001 Msa0667030:five_prime_UTR 30.0%
ATTGTAGAAAGAAGGAGAAA+TGG + chr4_4:7254990-7255009 Msa0667030:exon 30.0%
AATGGATCAAGTATTCAACA+AGG + chr4_4:7255008-7255027 Msa0667030:exon 30.0%
!! CAATTTTAAGTACTAGACCA+CGG - chr4_4:7255369-7255388 None:intergenic 30.0%
!!! TTTCCTTGTTTTTGTTGAAC+TGG - chr4_4:7255465-7255484 None:intergenic 30.0%
! TTGCTTGTCGTTAGAATTTT+AGG + chr4_4:7255618-7255637 Msa0667030:intron 30.0%
ATTCATCAAAAACTAAGAGC+TGG - chr4_4:7255706-7255725 None:intergenic 30.0%
TGATGAATAAGTCATGACAT+CGG + chr4_4:7255718-7255737 Msa0667030:intron 30.0%
CCACATCTACAAGTAAAATT+AGG - chr4_4:7255766-7255785 None:intergenic 30.0%
! CCTAATTTTACTTGTAGATG+TGG + chr4_4:7255763-7255782 Msa0667030:intron 30.0%
TTATCTCTAACAGGAAGATA+AGG - chr4_4:7256033-7256052 None:intergenic 30.0%
CATTACTCATTATCTCTAAC+AGG - chr4_4:7256042-7256061 None:intergenic 30.0%
TTTGAGCAACATATGGAAAT+AGG - chr4_4:7256250-7256269 None:intergenic 30.0%
!!! ACATTTATCTTTTCAAGTCC+TGG + chr4_4:7256269-7256288 Msa0667030:intron 30.0%
TGAAATCTTAAATGTTGCAC+AGG - chr4_4:7256311-7256330 None:intergenic 30.0%
! TTTCTCAATTTGGGATTACA+AGG - chr4_4:7256343-7256362 None:intergenic 30.0%
CAGAAAGGTAAGGAAAAAAT+TGG - chr4_4:7256477-7256496 None:intergenic 30.0%
TGATGTAATTACATGCAATG+GGG - chr4_4:7256679-7256698 None:intergenic 30.0%
GTTGATGTAATTACATGCAA+TGG - chr4_4:7256681-7256700 None:intergenic 30.0%
! TTACTGACTAACAGTTCATA+CGG - chr4_4:7257006-7257025 None:intergenic 30.0%
ATGTTCATACTTATTGCTTG+AGG - chr4_4:7257227-7257246 None:intergenic 30.0%
TAAGTATGAACATCATGAAG+GGG + chr4_4:7257234-7257253 Msa0667030:intron 30.0%
GAAATGGAGAGAATAAACAA+TGG - chr4_4:7257272-7257291 None:intergenic 30.0%
! AACTGTAGTAATGGAAGAAA+TGG - chr4_4:7257288-7257307 None:intergenic 30.0%
AACTGTGATTTCAAATCATG+TGG - chr4_4:7257363-7257382 None:intergenic 30.0%
GGTCTATAAAGAAACAACAA+AGG - chr4_4:7257887-7257906 None:intergenic 30.0%
! ATCAAGTAACTGTTGCTATT+TGG + chr4_4:7258039-7258058 Msa0667030:three_prime_UTR 30.0%
TTTAGTATCAAGGATGTTCA+TGG - chr4_4:7258142-7258161 None:intergenic 30.0%
AAAACAGAAAACAGGGATGA+TGG - chr4_4:7254951-7254970 None:intergenic 35.0%
GATCAAGTATTCAACAAGGT+TGG + chr4_4:7255012-7255031 Msa0667030:CDS 35.0%
! TTGTTGGTAAGCAGATTCTA+TGG + chr4_4:7255331-7255350 Msa0667030:intron 35.0%
! CTTTTCAAAGTAGAAGCTCA+CGG + chr4_4:7255407-7255426 Msa0667030:intron 35.0%
GTACCAGTTCAACAAAAACA+AGG + chr4_4:7255459-7255478 Msa0667030:intron 35.0%
CAAGGAAAAATGAATACGTG+TGG + chr4_4:7255477-7255496 Msa0667030:intron 35.0%
TGTGGATGAGATATAGACAT+TGG + chr4_4:7255781-7255800 Msa0667030:intron 35.0%
AGTGATGCTGTATGAGAAAT+AGG + chr4_4:7255857-7255876 Msa0667030:intron 35.0%
GTTTGTAACAAAGCTAATGC+TGG - chr4_4:7256011-7256030 None:intergenic 35.0%
TCAAAACTGAACATGTGAGA+AGG - chr4_4:7256066-7256085 None:intergenic 35.0%
GACTGCATATCAAAAGAAGA+AGG - chr4_4:7256164-7256183 None:intergenic 35.0%
TACTTTGTTGACCAACCATT+TGG - chr4_4:7256213-7256232 None:intergenic 35.0%
! TGGTTGGTCAACAAAGTAAA+AGG + chr4_4:7256215-7256234 Msa0667030:CDS 35.0%
GAAAAAATTGGACATGAGAG+GGG - chr4_4:7256465-7256484 None:intergenic 35.0%
GGAAAAAATTGGACATGAGA+GGG - chr4_4:7256466-7256485 None:intergenic 35.0%
AGGAAAAAATTGGACATGAG+AGG - chr4_4:7256467-7256486 None:intergenic 35.0%
CTAACTCATCCCTACAAAAT+AGG - chr4_4:7256631-7256650 None:intergenic 35.0%
! CTATTTTGTAGGGATGAGTT+AGG + chr4_4:7256629-7256648 Msa0667030:intron 35.0%
TAATTACATGCAATGGGGAT+TGG - chr4_4:7256674-7256693 None:intergenic 35.0%
AACCATGTTTAGATGCTTTG+CGG + chr4_4:7256698-7256717 Msa0667030:intron 35.0%
TCGAAAGTCACCAATTACTA+AGG - chr4_4:7256742-7256761 None:intergenic 35.0%
! GTAATTGGTGACTTTCGAAA+CGG + chr4_4:7256744-7256763 Msa0667030:intron 35.0%
TATGTGCCCTAATCTTACAA+TGG + chr4_4:7256768-7256787 Msa0667030:intron 35.0%
GGAGATCCATTGTAAGATTA+GGG - chr4_4:7256777-7256796 None:intergenic 35.0%
TGGAGATCCATTGTAAGATT+AGG - chr4_4:7256778-7256797 None:intergenic 35.0%
TTTAGTACTTTGAACCATGG+AGG + chr4_4:7256831-7256850 Msa0667030:intron 35.0%
AACTTATAAACAGACCTCCA+TGG - chr4_4:7256848-7256867 None:intergenic 35.0%
AAATAAAGCAGAACCATTGG+TGG - chr4_4:7257167-7257186 None:intergenic 35.0%
GAGAAATAAAGCAGAACCAT+TGG - chr4_4:7257170-7257189 None:intergenic 35.0%
! ATTGCTTGAGGAAAACCTTT+TGG - chr4_4:7257215-7257234 None:intergenic 35.0%
GATTTCAAATCATGTGGCAA+AGG - chr4_4:7257357-7257376 None:intergenic 35.0%
TCCTTTAACAACTCTGGTAA+TGG - chr4_4:7257485-7257504 None:intergenic 35.0%
TCATACTCCTTTAACAACTC+TGG - chr4_4:7257491-7257510 None:intergenic 35.0%
! AAGGAGTATGATCTACCAAT+AGG + chr4_4:7257500-7257519 Msa0667030:CDS 35.0%
CTACGAATTTAACGAAGAGA+CGG + chr4_4:7257547-7257566 Msa0667030:CDS 35.0%
TACGAATTTAACGAAGAGAC+GGG + chr4_4:7257548-7257567 Msa0667030:CDS 35.0%
CATGAAGAAAACAAGGAGTA+GGG + chr4_4:7257769-7257788 Msa0667030:CDS 35.0%
GCATATGATGTTACAAGAGA+TGG + chr4_4:7257794-7257813 Msa0667030:CDS 35.0%
CCATACAAGAAGACTCAAAA+TGG - chr4_4:7257908-7257927 None:intergenic 35.0%
!! CCATTTTGAGTCTTCTTGTA+TGG + chr4_4:7257905-7257924 Msa0667030:three_prime_UTR 35.0%
TTTGAGTCTTCTTGTATGGT+TGG + chr4_4:7257909-7257928 Msa0667030:three_prime_UTR 35.0%
TTGAGTCTTCTTGTATGGTT+GGG + chr4_4:7257910-7257929 Msa0667030:three_prime_UTR 35.0%
!! GTATTTCAAGTACTGATCCT+TGG + chr4_4:7257960-7257979 Msa0667030:three_prime_UTR 35.0%
ATTTCTCAAATCACAACCCA+AGG - chr4_4:7257987-7258006 None:intergenic 35.0%
TATCAAGGATGTTCATGGTT+CGG - chr4_4:7258137-7258156 None:intergenic 35.0%
TCAACAAGGTTGGATCCTAT+TGG + chr4_4:7255022-7255041 Msa0667030:CDS 40.0%
GGCTTTCTGATTGAACCAAT+AGG - chr4_4:7255040-7255059 None:intergenic 40.0%
AGCAAGGAGCTTAATTCTGT+CGG + chr4_4:7255060-7255079 Msa0667030:CDS 40.0%
!! TTTTAAGTACTAGACCACGG+TGG - chr4_4:7255366-7255385 None:intergenic 40.0%
! CGTGAGCTTCTACTTTGAAA+AGG - chr4_4:7255409-7255428 None:intergenic 40.0%
TTGTCTAACCGATAACGATC+AGG + chr4_4:7255536-7255555 Msa0667030:intron 40.0%
ACCGTTAACTCTTACTTCAG+AGG + chr4_4:7255588-7255607 Msa0667030:intron 40.0%
!! GCCTCTGAAGTAAGAGTTAA+CGG - chr4_4:7255592-7255611 None:intergenic 40.0%
!!! TTTTAAGTACTGGTCCACTG+TGG + chr4_4:7255644-7255663 Msa0667030:intron 40.0%
TGGTAAATGCAACACCAACA+GGG - chr4_4:7255686-7255705 None:intergenic 40.0%
AATAAGTCATGACATCGGTG+AGG + chr4_4:7255723-7255742 Msa0667030:intron 40.0%
ATAAGTCATGACATCGGTGA+GGG + chr4_4:7255724-7255743 Msa0667030:intron 40.0%
AGATGATTCTGTTACGGTGA+GGG + chr4_4:7255831-7255850 Msa0667030:intron 40.0%
GCTAATGCTGGATTATGACA+CGG - chr4_4:7255999-7256018 None:intergenic 40.0%
GGATCAAAATACTCGCAGTT+AGG + chr4_4:7256376-7256395 Msa0667030:intron 40.0%
! TTTTTCTGTGCATCAGCGTA+GGG + chr4_4:7256416-7256435 Msa0667030:intron 40.0%
TCAATGCGAGACTCAACAAA+GGG - chr4_4:7256552-7256571 None:intergenic 40.0%
TTCAATGCGAGACTCAACAA+AGG - chr4_4:7256553-7256572 None:intergenic 40.0%
! AACCGCAAAGCATCTAAACA+TGG - chr4_4:7256703-7256722 None:intergenic 40.0%
CTAAACCAGTTTGACACTAG+TGG + chr4_4:7257129-7257148 Msa0667030:intron 40.0%
TCATACCACTAGTGTCAAAC+TGG - chr4_4:7257137-7257156 None:intergenic 40.0%
ATGAACATCATGAAGGGGTT+AGG + chr4_4:7257239-7257258 Msa0667030:intron 40.0%
GGAGAGAATAAACAATGGAG+AGG - chr4_4:7257267-7257286 None:intergenic 40.0%
GCCATTACCAGAGTTGTTAA+AGG + chr4_4:7257481-7257500 Msa0667030:CDS 40.0%
TCACGAGGAAAGATTCCTAT+TGG - chr4_4:7257518-7257537 None:intergenic 40.0%
! GTTACTGGTTATCTGGAGAA+AGG + chr4_4:7257647-7257666 Msa0667030:CDS 40.0%
GCATGAAGAAAACAAGGAGT+AGG + chr4_4:7257768-7257787 Msa0667030:CDS 40.0%
GGGTTGTGATTTGAGAAATG+TGG + chr4_4:7257988-7258007 Msa0667030:three_prime_UTR 40.0%
GCTAAACGTGATACTCAACA+CGG - chr4_4:7258109-7258128 None:intergenic 40.0%
ATCAAGGATGTTCATGGTTC+GGG - chr4_4:7258136-7258155 None:intergenic 40.0%
AAGTTGGCTGCTTATTCGCT+AGG - chr4_4:7254914-7254933 None:intergenic 45.0%
TAGCGAATAAGCAGCCAACT+TGG + chr4_4:7254913-7254932 Msa0667030:five_prime_UTR 45.0%
GGACAACAACGATACCAAGT+TGG - chr4_4:7254930-7254949 None:intergenic 45.0%
GTTCAATCAGAAAGCCAGCA+AGG + chr4_4:7255044-7255063 Msa0667030:CDS 45.0%
GACAGAATTAAGCTCCTTGC+TGG - chr4_4:7255061-7255080 None:intergenic 45.0%
GCGTGTTTCCAATTTCCATG+AGG - chr4_4:7255191-7255210 None:intergenic 45.0%
GACAGTGACCTGATCGTTAT+CGG - chr4_4:7255547-7255566 None:intergenic 45.0%
! TCTTAGCCATGATGACGAGT+AGG + chr4_4:7255565-7255584 Msa0667030:intron 45.0%
TAAATGCAACACCAACAGGG+TGG - chr4_4:7255683-7255702 None:intergenic 45.0%
CTGGTAAATGCAACACCAAC+AGG - chr4_4:7255687-7255706 None:intergenic 45.0%
TAAGTCATGACATCGGTGAG+GGG + chr4_4:7255725-7255744 Msa0667030:intron 45.0%
GAGATGATTCTGTTACGGTG+AGG + chr4_4:7255830-7255849 Msa0667030:intron 45.0%
ATGCAGTTTGAGTGCGTTGT+AGG + chr4_4:7255910-7255929 Msa0667030:intron 45.0%
! TCACTGTCGAACAGTATCGA+AGG + chr4_4:7256182-7256201 Msa0667030:CDS 45.0%
! GTTTTTCTGTGCATCAGCGT+AGG + chr4_4:7256415-7256434 Msa0667030:intron 45.0%
ATGAGAGGGGTGAGAATACA+GGG - chr4_4:7256452-7256471 None:intergenic 45.0%
CGAGAGAAGACAGAAAGGTA+AGG - chr4_4:7256487-7256506 None:intergenic 45.0%
AGAGACGAGAGAAGACAGAA+AGG - chr4_4:7256492-7256511 None:intergenic 45.0%
TACATGCAATGGGGATTGGA+AGG - chr4_4:7256670-7256689 None:intergenic 45.0%
ATGGATCTCCACAGTTAGTG+TGG + chr4_4:7256787-7256806 Msa0667030:intron 45.0%
GTATGAAGCCAAACCACCAA+TGG + chr4_4:7257151-7257170 Msa0667030:intron 45.0%
! AAGCAGAACCATTGGTGGTT+TGG - chr4_4:7257162-7257181 None:intergenic 45.0%
!! TCGTAGTTTGTTGCATCACG+AGG - chr4_4:7257533-7257552 None:intergenic 45.0%
GCTTGAAGTCTTCATTCCTC+AGG + chr4_4:7257574-7257593 Msa0667030:CDS 45.0%
AGTGGTGAAGAAACGCAAGA+CGG - chr4_4:7257627-7257646 None:intergenic 45.0%
CGTTTCTTCACCACTGTTAC+TGG + chr4_4:7257632-7257651 Msa0667030:CDS 45.0%
! CACCACTGTTACTGGTTATC+TGG + chr4_4:7257640-7257659 Msa0667030:CDS 45.0%
CTCCAGATAACCAGTAACAG+TGG - chr4_4:7257645-7257664 None:intergenic 45.0%
AGGAAAGCTAGCAGACATAG+AGG + chr4_4:7257667-7257686 Msa0667030:CDS 45.0%
GGAAAGCTAGCAGACATAGA+GGG + chr4_4:7257668-7257687 Msa0667030:CDS 45.0%
! AGTGACAACCATTTTCTCCG+AGG + chr4_4:7257721-7257740 Msa0667030:CDS 45.0%
! GTGACAACCATTTTCTCCGA+GGG + chr4_4:7257722-7257741 Msa0667030:CDS 45.0%
CTGCTGGCATGAAGAAAACA+AGG + chr4_4:7257762-7257781 Msa0667030:CDS 45.0%
AGTCTTCTTGTATGGTTGGG+TGG + chr4_4:7257913-7257932 Msa0667030:three_prime_UTR 45.0%
!! AAGTACTGATCCTTGGACCT+TGG + chr4_4:7257967-7257986 Msa0667030:three_prime_UTR 45.0%
!! AGTACTGATCCTTGGACCTT+GGG + chr4_4:7257968-7257987 Msa0667030:three_prime_UTR 45.0%
AAATCACAACCCAAGGTCCA+AGG - chr4_4:7257980-7257999 None:intergenic 45.0%
! TACTCAACACGGTTTGGGTT+TGG - chr4_4:7258098-7258117 None:intergenic 45.0%
CGTGATACTCAACACGGTTT+GGG - chr4_4:7258103-7258122 None:intergenic 45.0%
ACGTGATACTCAACACGGTT+TGG - chr4_4:7258104-7258123 None:intergenic 45.0%
ATCAAGTCACCGTCCTAGCT+AGG - chr4_4:7255437-7255456 None:intergenic 50.0%
AGCTAGGACGGTGACTTGAT+CGG + chr4_4:7255437-7255456 Msa0667030:intron 50.0%
AACGGTCCTACTCGTCATCA+TGG - chr4_4:7255574-7255593 None:intergenic 50.0%
GATTCTATGGTGGTCCACAG+TGG - chr4_4:7255661-7255680 None:intergenic 50.0%
! CAACAGGGTGGATTCTATGG+TGG - chr4_4:7255671-7255690 None:intergenic 50.0%
CACCATAGAATCCACCCTGT+TGG + chr4_4:7255669-7255688 Msa0667030:intron 50.0%
! CACCAACAGGGTGGATTCTA+TGG - chr4_4:7255674-7255693 None:intergenic 50.0%
TTAAGCAAGGGAAAGACGCG+AGG + chr4_4:7255948-7255967 Msa0667030:intron 50.0%
! CTGTCGAACAGTATCGAAGG+CGG + chr4_4:7256185-7256204 Msa0667030:CDS 50.0%
GTATCGAAGGCGGAACCAAA+TGG + chr4_4:7256195-7256214 Msa0667030:CDS 50.0%
!!! CTTTTCAAGTCCTGGACTCC+TGG + chr4_4:7256277-7256296 Msa0667030:intron 50.0%
GTTGCACAGGTTTCATAGCC+AGG - chr4_4:7256298-7256317 None:intergenic 50.0%
CATGAGAGGGGTGAGAATAC+AGG - chr4_4:7256453-7256472 None:intergenic 50.0%
TGCTCAGTCCACACTAACTG+TGG - chr4_4:7256798-7256817 None:intergenic 50.0%
ATTCCTCAGGTCTGCGAAGT+AGG + chr4_4:7257587-7257606 Msa0667030:CDS 50.0%
GGTCTGCGAAGTAGGCTATA+AGG + chr4_4:7257595-7257614 Msa0667030:CDS 50.0%
CTTCGATCCCTCGGAGAAAA+TGG - chr4_4:7257732-7257751 None:intergenic 50.0%
CACATAGAGCTTCGATCCCT+CGG - chr4_4:7257741-7257760 None:intergenic 50.0%
TCGAAGCTCTATGTGACTGC+TGG + chr4_4:7257746-7257765 Msa0667030:CDS 50.0%
! GGATGTTCATGGTTCGGGTT+CGG - chr4_4:7258131-7258150 None:intergenic 50.0%
TATGGTCACGTTGTCCACCG+TGG + chr4_4:7255349-7255368 Msa0667030:intron 55.0%
AAGTCACCGTCCTAGCTAGG+AGG - chr4_4:7255434-7255453 None:intergenic 55.0%
GACATCGGTGAGGGGAAATG+TGG + chr4_4:7255733-7255752 Msa0667030:intron 55.0%
GACGCGAGGATCTGCAACTT+AGG + chr4_4:7255962-7255981 Msa0667030:intron 55.0%
CGAAGGCGGAACCAAATGGT+TGG + chr4_4:7256199-7256218 Msa0667030:CDS 55.0%
GGTTTCATAGCCAGGAGTCC+AGG - chr4_4:7256290-7256309 None:intergenic 55.0%
TAGCCTACTTCGCAGACCTG+AGG - chr4_4:7257593-7257612 None:intergenic 55.0%
AGCTCACGGTCCTCCTAGCT+AGG + chr4_4:7255421-7255440 Msa0667030:intron 60.0%
CACGGTCCTCCTAGCTAGGA+CGG + chr4_4:7255425-7255444 Msa0667030:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 7254896 7258196 7254896 ID=Msa0667030;Name=Msa0667030
chr4_4 mRNA 7254896 7258196 7254896 ID=Msa0667030-mRNA-1;Parent=Msa0667030;Name=Msa0667030-mRNA-1;_AED=0.01;_eAED=0.01;_QI=113|1|1|1|1|1|3|358|170
chr4_4 exon 7254896 7255095 7254896 ID=Msa0667030-mRNA-1:exon:2344;Parent=Msa0667030-mRNA-1
chr4_4 exon 7256182 7256236 7256182 ID=Msa0667030-mRNA-1:exon:2345;Parent=Msa0667030-mRNA-1
chr4_4 exon 7257468 7258196 7257468 ID=Msa0667030-mRNA-1:exon:2346;Parent=Msa0667030-mRNA-1
chr4_4 five_prime_UTR 7254896 7255008 7254896 ID=Msa0667030-mRNA-1:five_prime_utr;Parent=Msa0667030-mRNA-1
chr4_4 CDS 7255009 7255095 7255009 ID=Msa0667030-mRNA-1:cds;Parent=Msa0667030-mRNA-1
chr4_4 CDS 7256182 7256236 7256182 ID=Msa0667030-mRNA-1:cds;Parent=Msa0667030-mRNA-1
chr4_4 CDS 7257468 7257838 7257468 ID=Msa0667030-mRNA-1:cds;Parent=Msa0667030-mRNA-1
chr4_4 three_prime_UTR 7257839 7258196 7257839 ID=Msa0667030-mRNA-1:three_prime_utr;Parent=Msa0667030-mRNA-1
Gene Sequence

>Msa0667030

ATGGATCAAGTATTCAACAAGGTTGGATCCTATTGGTTCAATCAGAAAGCCAGCAAGGAGCTTAATTCTGTCGGTGATGAAATCAATTCACTGTCGAACAGTATCGAAGGCGGAACCAAATGGTTGGTCAACAAAGTAAAAGGAAAAATGCAGAAGCCATTACCAGAGTTGTTAAAGGAGTATGATCTACCAATAGGAATCTTTCCTCGTGATGCAACAAACTACGAATTTAACGAAGAGACGGGAAAGCTTGAAGTCTTCATTCCTCAGGTCTGCGAAGTAGGCTATAAGGATTCATCCGTCTTGCGTTTCTTCACCACTGTTACTGGTTATCTGGAGAAAGGAAAGCTAGCAGACATAGAGGGAATGAAGACAAAAGTTATTATCTGGGTAAAAGTGACAACCATTTTCTCCGAGGGATCGAAGCTCTATGTGACTGCTGGCATGAAGAAAACAAGGAGTAGGGAAGCATATGATGTTACAAGAGATGGCGTACCTGTAGATAAGTTCTAA
Protein sequence

>Msa0667030

MDQVFNKVGSYWFNQKASKELNSVGDEINSLSNSIEGGTKWLVNKVKGKMQKPLPELLKEYDLPIGIFPRDATNYEFNEETGKLEVFIPQVCEVGYKDSSVLRFFTTVTGYLEKGKLADIEGMKTKVIIWVKVTTIFSEGSKLYVTAGMKKTRSREAYDVTRDGVPVDKF*