AlfalfaGEDB | Msa0632420

M. sativa cultivar zhongmu-4 / Msa0632420

KEGG: K08511|K08515
GO: GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0097708 GO:0098796
PFAM: PF13774.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632420	XP_003604990.1	100.000	219	0	0	1	219	1	219	6.51e-162	456

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632420	sp\|Q9MAS5\|VA726_ARATH	80.365	219	43	0	1	219	1	219	2.21e-132	374

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632420	G7JUL3	100.000	219	0	0	1	219	1	219	3.11e-162	456

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0032710	Msa0632420	0.801419	9.085245e-49	-8.615850e-47
Msa0043850	Msa0632420	0.826859	2.152683e-54	-8.615850e-47
Msa0061220	Msa0632420	0.828308	9.664975e-55	-8.615850e-47
Msa0076290	Msa0632420	0.808189	3.499740e-50	-8.615850e-47
Msa0077370	Msa0632420	0.805732	1.158188e-49	-8.615850e-47
Msa0082420	Msa0632420	0.815247	1.019622e-51	-8.615850e-47
Msa0090510	Msa0632420	0.816452	5.490716e-52	-8.615850e-47
Msa0097570	Msa0632420	0.811170	8.003002e-51	-8.615850e-47
Msa0137980	Msa0632420	0.821763	3.396168e-53	-8.615850e-47
Msa0139670	Msa0632420	0.830647	2.610650e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632420	MtrunA17_Chr4g0010271	100.000	219	0	0	1	219	1	219	5.99e-166	456
Msa0632420	MtrunA17_Chr1g0169861	78.995	219	46	0	1	219	51	269	2.50e-138	388
Msa0632420	MtrunA17_Chr7g0237561	84.018	219	35	0	1	219	1	219	1.72e-129	363
Msa0632420	MtrunA17_Chr2g0296021	71.233	219	63	0	1	219	1	219	1.10e-122	346
Msa0632420	MtrunA17_Chr4g0043521	79.275	193	40	0	3	195	5	197	3.93e-121	342
Msa0632420	MtrunA17_Chr2g0291651	74.419	215	55	0	2	216	6	220	5.40e-115	327
Msa0632420	MtrunA17_Chr7g0237521	75.385	195	48	0	1	195	1	195	1.39e-111	318
Msa0632420	MtrunA17_Chr1g0210521	56.303	238	85	1	1	219	1	238	7.10e-102	294
Msa0632420	MtrunA17_Chr4g0010211	95.370	108	5	0	64	171	3	110	1.90e-71	213
Msa0632420	MtrunA17_Chr1g0169851	69.178	146	30	1	57	187	8	153	4.04e-70	211

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0632420	AT1G04760.2	80.365	219	43	0	1	219	1	219	2.25e-133	374
Msa0632420	AT1G04760.1	80.365	219	43	0	1	219	1	219	2.25e-133	374
Msa0632420	AT1G04750.1	83.105	219	37	0	1	219	1	219	3.29e-129	363
Msa0632420	AT2G32670.1	82.192	219	39	0	1	219	66	284	5.30e-129	365
Msa0632420	AT2G33120.1	83.105	219	37	0	1	219	1	219	1.24e-128	362
Msa0632420	AT2G33120.2	79.736	227	38	1	1	219	1	227	2.07e-125	354
Msa0632420	AT3G54300.3	56.723	238	84	2	1	219	1	238	8.02e-101	292
Msa0632420	AT3G54300.2	56.723	238	84	2	1	219	1	238	8.02e-101	292
Msa0632420	AT3G54300.1	56.723	238	84	2	1	219	1	238	8.02e-101	292
Msa0632420	AT2G33110.1	67.580	219	67	1	1	219	1	215	5.90e-97	281

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Find 66 sgRNAs with CRISPR-Local

Find 406 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCTTAACAAAGAATT+TGG	0.112791	4_3:-16386644	Msa0632420:CDS
AAAATGATGAAGAAATGAAA+AGG	0.501975	4_3:+16388242	None:intergenic
AAACTGTATTGTTTCATTTA+TGG	0.092767	4_3:-16384354	Msa0632420:three_prime_UTR
AAAGGAGTGAAAACAGGAAA+AGG	0.500312	4_3:+16388307	None:intergenic
AAAGTCTTAACAAAGAATTT+GGG	0.498770	4_3:-16386643	Msa0632420:intron
AACAATACAGTTTAAAGAGT+AGG	0.602534	4_3:+16384364	None:intergenic
AACTCCCTGCTTCCAACAAC+AGG	0.382219	4_3:-16388023	Msa0632420:CDS
AACTGAGAACCTTCGCTCTC+AGG	0.417900	4_3:-16385340	Msa0632420:intron
AAGACACTGTAAAGCAATGG+TGG	0.700756	4_3:+16388050	None:intergenic
ACCACATCTTTCTTCTCAAC+TGG	0.279528	4_3:+16384661	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTGAATTGTGATCATTCA+TGG	-	chr4_3:16384407-16384426	Msa0632420:three_prime_UTR	30.0%
TGTGAATTGTGATCATTCAT+GGG	-	chr4_3:16384408-16384427	Msa0632420:three_prime_UTR	30.0%
TATGTGGAACAATAACTAGT+TGG	-	chr4_3:16385162-16385181	Msa0632420:intron	30.0%
TGCATAAAATATGCAAGGTT+CGG	+	chr4_3:16385402-16385421	None:intergenic	30.0%
TGAACATGTTTCATGATTAG+TGG	-	chr4_3:16385765-16385784	Msa0632420:intron	30.0%
ATACGTTCAAGAAAAGCTAT+TGG	+	chr4_3:16385842-16385861	None:intergenic	30.0%
TTTAGATTGAAGAACCGAAT+TGG	-	chr4_3:16386276-16386295	Msa0632420:intron	30.0%
AAAAATCCATGCAAAATCGT+AGG	-	chr4_3:16386442-16386461	Msa0632420:CDS	30.0%
TATTTATAGAACAGGATCAC+AGG	+	chr4_3:16386541-16386560	None:intergenic	30.0%
TATGGCTACTAAAGAATATC+TGG	-	chr4_3:16386829-16386848	Msa0632420:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	16384242	16388341	16384242	ID=Msa0632420;Name=Msa0632420
chr4_3	mRNA	16384242	16388341	16384242	ID=Msa0632420-mRNA-1;Parent=Msa0632420;Name=Msa0632420-mRNA-1;_AED=0.10;_eAED=0.10;_QI=184\|1\|1\|1\|1\|1\|5\|324\|219
chr4_3	exon	16384242	16384709	16384242	ID=Msa0632420-mRNA-1:exon:4646;Parent=Msa0632420-mRNA-1
chr4_3	exon	16385341	16385403	16385341	ID=Msa0632420-mRNA-1:exon:4645;Parent=Msa0632420-mRNA-1
chr4_3	exon	16386438	16386558	16386438	ID=Msa0632420-mRNA-1:exon:4644;Parent=Msa0632420-mRNA-1
chr4_3	exon	16386644	16386785	16386644	ID=Msa0632420-mRNA-1:exon:4643;Parent=Msa0632420-mRNA-1
chr4_3	exon	16387968	16388341	16387968	ID=Msa0632420-mRNA-1:exon:4642;Parent=Msa0632420-mRNA-1
chr4_3	five_prime_UTR	16388158	16388341	16388158	ID=Msa0632420-mRNA-1:five_prime_utr;Parent=Msa0632420-mRNA-1
chr4_3	CDS	16387968	16388157	16387968	ID=Msa0632420-mRNA-1:cds;Parent=Msa0632420-mRNA-1
chr4_3	CDS	16386644	16386785	16386644	ID=Msa0632420-mRNA-1:cds;Parent=Msa0632420-mRNA-1
chr4_3	CDS	16386438	16386558	16386438	ID=Msa0632420-mRNA-1:cds;Parent=Msa0632420-mRNA-1
chr4_3	CDS	16385341	16385403	16385341	ID=Msa0632420-mRNA-1:cds;Parent=Msa0632420-mRNA-1
chr4_3	CDS	16384566	16384709	16384566	ID=Msa0632420-mRNA-1:cds;Parent=Msa0632420-mRNA-1
chr4_3	three_prime_UTR	16384242	16384565	16384242	ID=Msa0632420-mRNA-1:three_prime_utr;Parent=Msa0632420-mRNA-1

Gene Sequence

>Msa0632420

ATGGGACAACAATCATTGATCTATAGCTTTGTGGCTCGTGGTACCGTGATTCTTGCGGAGTACAGTGACTTCACAGGAAACTTCACCACCATTGCTTTACAGTGTCTTCAAAAACTCCCTGCTTCCAACAACAGGTTTACTTACAACTGTGATGGACATACCTTCAGCTTCCTCGTCGATAATGGATTCACTTACTGTGTTGTGGCAGTGGAGTCTGTTGGTAGACAAATTCCAATAGCTTTTCTTGAACGTATTAAGGATGATTTTAATAAAAGATATGGTGGAGGAAGAGCTACAACAGCAACTGCTAAAAGTCTTAACAAAGAATTTGGACCAAAGTTGAAGGAGCAGATGCAATATTGTGTGGAACATCCAGAGGAAGTTAGCAAACTTGCTAAAGTGAAAGCTCAAGTTTCTGAAGTCAAAGGAGTTATGATGGAAAATATCGACAAGGTTATTGATAGGGGAGAGAAGATTGAGGTTTTGGTGGATAAAACTGAGAACCTTCGCTCTCAGGCACAAGATTTCAGGCAGCAGGGAACCCAGTTGAGAAGAAAGATGTGGTATCAAAACATGAAGATCAAGCTAATAGTCCTTGCTATCATAATTGCATTGATTCTCATTATTGTTCTTTCTGTGTGCCATGGCTTCAGTTGTTAA

Protein sequence

>Msa0632420

MGQQSLIYSFVARGTVILAEYSDFTGNFTTIALQCLQKLPASNNRFTYNCDGHTFSFLVDNGFTYCVVAVESVGRQIPIAFLERIKDDFNKRYGGGRATTATAKSLNKEFGPKLKEQMQYCVEHPEEVSKLAKVKAQVSEVKGVMMENIDKVIDRGEKIEVLVDKTENLRSQAQDFRQQGTQLRRKMWYQNMKIKLIVLAIIIALILIIVLSVCHGFSC*