AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0532230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532230 MtrunA17_Chr4g0005561 100.000 170 0 0 1 170 1 170 4.65e-124 346
Msa0532230 MtrunA17_Chr7g0243261 84.706 170 26 0 1 170 1 170 1.51e-104 296
Msa0532230 MtrunA17_Chr5g0418311 51.205 166 78 1 8 170 15 180 1.51e-59 183
Msa0532230 MtrunA17_Chr4g0061181 46.552 174 89 1 1 170 1 174 1.47e-55 172
Msa0532230 MtrunA17_Chr7g0269431 41.772 79 44 2 56 133 33 110 7.92e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532230 AT3G08890.1 77.647 170 38 0 1 170 1 170 8.42e-100 285
Msa0532230 AT3G08890.2 77.647 170 38 0 1 170 1 170 8.42e-100 285
Msa0532230 AT5G37070.1 78.824 170 36 0 1 170 1 170 1.06e-98 282
Msa0532230 AT5G01610.2 75.882 170 41 0 1 170 1 170 2.23e-96 276
Msa0532230 AT5G01610.1 75.882 170 41 0 1 170 1 170 2.23e-96 276
Msa0532230 AT2G03350.1 50.000 164 79 1 7 167 12 175 2.94e-53 167
Msa0532230 AT1G02816.1 25.217 115 83 2 57 168 35 149 1.54e-12 62.8
Msa0532230 AT4G02370.1 27.368 95 68 1 56 149 34 128 1.39e-11 60.1
Msa0532230 AT4G02360.1 29.358 109 74 3 51 157 25 132 2.03e-11 59.3

Find 69 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTCTTCACCACTGTTAC+TGG 0.210400 4_1:+6245461 Msa0532230:CDS
TCATATGATATATTGTTATT+AGG 0.213758 4_1:+6245673 Msa0532230:three_prime_UTR
ATCAAGTAACTGTTGCTATT+TGG 0.237961 4_1:+6245868 Msa0532230:three_prime_UTR
CACCACTGTTACTGGTTATC+TGG 0.249379 4_1:+6245469 Msa0532230:CDS
TGAAGACAAAAGTTATTATC+TGG 0.261455 4_1:+6245522 Msa0532230:CDS
CTTCGATCCCTCGGAGAAAA+TGG 0.294455 4_1:-6245558 None:intergenic
GCCATTACCAGAGTTGTTAA+AGG 0.295014 4_1:+6245310 Msa0532230:CDS
ATCAAGGATGTTCATGGTTC+GGG 0.298355 4_1:-6245962 None:intergenic
TGGTTGGTCAACAAAGTAAA+AGG 0.300821 4_1:+6244041 Msa0532230:CDS
TACTTTGTTGACCAACCATT+TGG 0.339849 4_1:-6244036 None:intergenic
AGTACTGATCCTTGGACCTT+GGG 0.351627 4_1:+6245797 Msa0532230:three_prime_UTR
CGTGATACTCAACACGGTTT+GGG 0.367490 4_1:-6245929 None:intergenic
GGATGTTCATGGTTCGGGTT+TGG 0.368989 4_1:-6245957 None:intergenic
ACGTGATACTCAACACGGTT+TGG 0.373063 4_1:-6245930 None:intergenic
GACAGAATTAAGCTCCTTGC+TGG 0.374345 4_1:-6242889 None:intergenic
TACTCAACACGGTTTGGGTT+TGG 0.388586 4_1:-6245924 None:intergenic
ATTGTAGAAAGAAGGAGAAA+TGG 0.390997 4_1:+6242821 Msa0532230:exon
TATCAAGGATGTTCATGGTT+CGG 0.402009 4_1:-6245963 None:intergenic
TCAACAAGGTTGGATCCTAT+TGG 0.402846 4_1:+6242853 Msa0532230:CDS
TCCTTTAACAACTCTGGTAA+TGG 0.418016 4_1:-6245311 None:intergenic
AAGTACTGATCCTTGGACCT+TGG 0.424944 4_1:+6245796 Msa0532230:three_prime_UTR
GGCTTTCTGATTGAACCAAT+AGG 0.431334 4_1:-6242868 None:intergenic
GGTCTGCGAAGTAGGCTATA+AGG 0.432562 4_1:+6245424 Msa0532230:CDS
TTGAGTCTTCTTGTATGGTT+GGG 0.436411 4_1:+6245739 Msa0532230:three_prime_UTR
CCATACAAGAAGACTCAAAA+TGG 0.436737 4_1:-6245734 None:intergenic
TTTGAGTCTTCTTGTATGGT+TGG 0.437027 4_1:+6245738 Msa0532230:three_prime_UTR
TCTAGTATCAAGGATGTTCA+TGG 0.437093 4_1:-6245968 None:intergenic
AAGGAGTATGATCTACCAAT+AGG 0.443273 4_1:+6245329 Msa0532230:CDS
TCTTTCTGATTGTAGAAAGA+AGG 0.444898 4_1:+6242813 Msa0532230:five_prime_UTR
AGGAAAGCTAGCAGACATAG+AGG 0.449690 4_1:+6245496 Msa0532230:CDS
ATTCCTCAGGTCTGCGAAGT+AGG 0.457321 4_1:+6245416 Msa0532230:CDS
GTATTTCAAGTACTGATCCT+TGG 0.461000 4_1:+6245789 Msa0532230:three_prime_UTR
CACATAGAGCTTCGATCCCT+CGG 0.465720 4_1:-6245567 None:intergenic
TCACGAGGAAAGATTCCTAT+TGG 0.469668 4_1:-6245344 None:intergenic
AAAACAGAAAACAGGGATGA+TGG 0.476992 4_1:-6242779 None:intergenic
GCTTGAAGTCTTCATTCCTC+AGG 0.483186 4_1:+6245403 Msa0532230:CDS
GATCAAGTATTCAACAAGGT+TGG 0.497292 4_1:+6242843 Msa0532230:CDS
GAAGACAAAAGTTATTATCT+GGG 0.508796 4_1:+6245523 Msa0532230:CDS
AAGTTGGCTGCTTATTCGCT+AGG 0.523175 4_1:-6242742 None:intergenic
GCATATGATGTTACAAGAGA+TGG 0.523208 4_1:+6245623 Msa0532230:CDS
TCGAAGCTCTATGTGACTGC+TGG 0.534882 4_1:+6245575 Msa0532230:CDS
GTATCGAAGGCGGAACCAAA+TGG 0.539035 4_1:+6244021 Msa0532230:CDS
TAGCGAATAAGCAGCCAACT+TGG 0.539393 4_1:+6242744 Msa0532230:five_prime_UTR
GTTACTGGTTATCTGGAGAA+AGG 0.540770 4_1:+6245476 Msa0532230:CDS
GCATGAAGAAAACAAGGAGT+AGG 0.544902 4_1:+6245597 Msa0532230:CDS
TCATACTCCTTTAACAACTC+TGG 0.548889 4_1:-6245317 None:intergenic
AGTCTTCTTGTATGGTTGGG+TGG 0.550498 4_1:+6245742 Msa0532230:three_prime_UTR
CTGCTGGCATGAAGAAAACA+AGG 0.561931 4_1:+6245591 Msa0532230:CDS
AAATTACTACTCTAGTATCA+AGG 0.571816 4_1:-6245978 None:intergenic
TACGAATTTAACGAAGAGAC+GGG 0.578620 4_1:+6245377 Msa0532230:CDS
CTACGAATTTAACGAAGAGA+CGG 0.579413 4_1:+6245376 Msa0532230:CDS
TCGTAGTTTGTTGCATCACG+AGG 0.584895 4_1:-6245359 None:intergenic
AAATCACAACCCAAGGTCCA+AGG 0.587281 4_1:-6245806 None:intergenic
AGCAAGGAGCTTAATTCTGT+CGG 0.592969 4_1:+6242891 Msa0532230:CDS
CATGAAGAAAACAAGGAGTA+GGG 0.600568 4_1:+6245598 Msa0532230:CDS
GGAAAGCTAGCAGACATAGA+GGG 0.604204 4_1:+6245497 Msa0532230:CDS
CGAAGGCGGAACCAAATGGT+TGG 0.621013 4_1:+6244025 Msa0532230:CDS
GTTCAATCAGAAAGCCAGCA+AGG 0.622010 4_1:+6242875 Msa0532230:CDS
GGACAACAACGATACCAAGT+TGG 0.626792 4_1:-6242758 None:intergenic
ATTTCTCAAATCACAACCCA+AGG 0.635629 4_1:-6245813 None:intergenic
GGGTTGTGATTTGAGAAATG+TGG 0.644526 4_1:+6245817 Msa0532230:three_prime_UTR
TCACTGTCGAACAGTATCGA+AGG 0.655695 4_1:+6244008 Msa0532230:CDS
TAGCCTACTTCGCAGACCTG+AGG 0.660590 4_1:-6245419 None:intergenic
CTCCAGATAACCAGTAACAG+TGG 0.669904 4_1:-6245471 None:intergenic
GGTCTATAAAGAAACAACAA+AGG 0.670169 4_1:-6245713 None:intergenic
GCTAAACGTGATACTCAACA+CGG 0.683885 4_1:-6245935 None:intergenic
AGTGGTGAAGAAACGCAAGA+CGG 0.697268 4_1:-6245453 None:intergenic
AATGGATCAAGTATTCAACA+AGG 0.730089 4_1:+6242839 Msa0532230:exon
CTGTCGAACAGTATCGAAGG+CGG 0.747673 4_1:+6244011 Msa0532230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAAATTATTAATTTT+TGG + chr4_1:6242987-6243006 Msa0532230:intron 10.0%
!!! TATCCAAAAATTAATAATTT+TGG - chr4_1:6242993-6243012 None:intergenic 10.0%
!! AAAAACTGAAAAATTGAAAA+GGG - chr4_1:6242945-6242964 None:intergenic 15.0%
!! AAAAAACTGAAAAATTGAAA+AGG - chr4_1:6242946-6242965 None:intergenic 15.0%
!! AAAATAATTTAACAGTGAAA+AGG + chr4_1:6244798-6244817 Msa0532230:intron 15.0%
!! TCTATTAACAAAATTATAGA+AGG - chr4_1:6244932-6244951 None:intergenic 15.0%
!!! TCATATGATATATTGTTATT+AGG + chr4_1:6245673-6245692 Msa0532230:three_prime_UTR 15.0%
!! AAAACTGAAAAATTGAAAAG+GGG - chr4_1:6242944-6242963 None:intergenic 20.0%
!! ATCAATTGAATTGAATTGAT+GGG + chr4_1:6243053-6243072 Msa0532230:intron 20.0%
!!! AAGATATCTGAATTTTGAAT+TGG + chr4_1:6243104-6243123 Msa0532230:intron 20.0%
!! TATTTAATTCTGATTAAGCA+AGG + chr4_1:6243761-6243780 Msa0532230:intron 20.0%
!! ATTTAATTCTGATTAAGCAA+GGG + chr4_1:6243762-6243781 Msa0532230:intron 20.0%
!! TTCTATCAATTTCTCAATTT+GGG - chr4_1:6244178-6244197 None:intergenic 20.0%
!! TTTCTATCAATTTCTCAATT+TGG - chr4_1:6244179-6244198 None:intergenic 20.0%
!!! TCACTTATAAATACATTTTC+AGG - chr4_1:6244412-6244431 None:intergenic 20.0%
!! TTAATATACACCTTAGTAAT+TGG + chr4_1:6244555-6244574 Msa0532230:intron 20.0%
!! ATAAATACATGTGTATTTGT+TGG + chr4_1:6245145-6245164 Msa0532230:intron 20.0%
!! AGAATAGATTTCTATATTGA+AGG + chr4_1:6245250-6245269 Msa0532230:intron 20.0%
!! GAATAGATTTCTATATTGAA+GGG + chr4_1:6245251-6245270 Msa0532230:intron 20.0%
! ATGAACAAAAACAGAAAACA+GGG - chr4_1:6242789-6242808 None:intergenic 25.0%
! AATGAACAAAAACAGAAAAC+AGG - chr4_1:6242790-6242809 None:intergenic 25.0%
!! TTTTGGATAATTAAACCTCA+TGG + chr4_1:6243004-6243023 Msa0532230:intron 25.0%
! TAATTAAACCTCATGGAAAT+TGG + chr4_1:6243011-6243030 Msa0532230:intron 25.0%
! GATCAATTGAATTGAATTGA+TGG + chr4_1:6243052-6243071 Msa0532230:intron 25.0%
! AACAAACATAAACATCATTG+TGG - chr4_1:6243142-6243161 None:intergenic 25.0%
! ACAATGATGTTTATGTTTGT+TGG + chr4_1:6243141-6243160 Msa0532230:intron 25.0%
!!! TTTTAGGTGATTTTAAGTAC+TGG + chr4_1:6243460-6243479 Msa0532230:intron 25.0%
! TTAATGAGATGATTCTGTTA+CGG + chr4_1:6243651-6243670 Msa0532230:intron 25.0%
! CATGTCAGTAAACATAATTT+AGG + chr4_1:6243711-6243730 Msa0532230:intron 25.0%
!! AAAGATGAATTTTCATTGCT+TGG + chr4_1:6243946-6243965 Msa0532230:intron 25.0%
! ATAAATGTTTGAGCAACATA+TGG - chr4_1:6244083-6244102 None:intergenic 25.0%
! TTGAGAAATTGATAGAAAAC+TGG + chr4_1:6244181-6244200 Msa0532230:intron 25.0%
!! AATTTACAAGCCTATTTTGT+AGG + chr4_1:6244444-6244463 Msa0532230:intron 25.0%
!! ATTTACAAGCCTATTTTGTA+GGG + chr4_1:6244445-6244464 Msa0532230:intron 25.0%
! TTGGAAGGAAAAATTGAATT+GGG - chr4_1:6244481-6244500 None:intergenic 25.0%
! ATTGGAAGGAAAAATTGAAT+TGG - chr4_1:6244482-6244501 None:intergenic 25.0%
! TTGATGTAATTACATGCAAT+GGG - chr4_1:6244506-6244525 None:intergenic 25.0%
! AAATTTAGTACTTTGAACCA+TGG + chr4_1:6244654-6244673 Msa0532230:intron 25.0%
!! GGTCTGTTTATAAGTTATTT+TGG + chr4_1:6244678-6244697 Msa0532230:intron 25.0%
!! CAAAAGACTTCTAAAAATTG+AGG - chr4_1:6244887-6244906 None:intergenic 25.0%
!!! CAATTTTTAGAAGTCTTTTG+AGG + chr4_1:6244887-6244906 Msa0532230:intron 25.0%
! ATAATAGACAATTCATGAAG+TGG - chr4_1:6245021-6245040 None:intergenic 25.0%
! TGAATTGTCTATTATCCAAA+AGG + chr4_1:6245026-6245045 Msa0532230:intron 25.0%
! AATAAGTATGAACATCATGA+AGG + chr4_1:6245061-6245080 Msa0532230:intron 25.0%
! ATAAGTATGAACATCATGAA+GGG + chr4_1:6245062-6245081 Msa0532230:intron 25.0%
!!! TATCTCTAAAACTGTAGTAA+TGG - chr4_1:6245126-6245145 None:intergenic 25.0%
! TCTATTTGACATAGCAATAA+TGG + chr4_1:6245209-6245228 Msa0532230:intron 25.0%
! ACTTAATTATATCACTTTGC+AGG + chr4_1:6245275-6245294 Msa0532230:intron 25.0%
! TGAAGACAAAAGTTATTATC+TGG + chr4_1:6245522-6245541 Msa0532230:CDS 25.0%
! GAAGACAAAAGTTATTATCT+GGG + chr4_1:6245523-6245542 Msa0532230:CDS 25.0%
!!! AAGTTTTAGAACTTATCTAC+AGG - chr4_1:6245653-6245672 None:intergenic 25.0%
! AAATTACTACTCTAGTATCA+AGG - chr4_1:6245981-6246000 None:intergenic 25.0%
TCTTTCTGATTGTAGAAAGA+AGG + chr4_1:6242813-6242832 Msa0532230:five_prime_UTR 30.0%
ATTGTAGAAAGAAGGAGAAA+TGG + chr4_1:6242821-6242840 Msa0532230:exon 30.0%
AATGGATCAAGTATTCAACA+AGG + chr4_1:6242839-6242858 Msa0532230:exon 30.0%
!! CAATTTTAAGTACTAGACCA+CGG - chr4_1:6243195-6243214 None:intergenic 30.0%
!!! TTTCCTTGTTTTTGTTGAAC+TGG - chr4_1:6243291-6243310 None:intergenic 30.0%
! TTGCTTGTCGTTAGAATTTT+AGG + chr4_1:6243444-6243463 Msa0532230:intron 30.0%
ATTCATCAAAAACTAAGAGC+TGG - chr4_1:6243532-6243551 None:intergenic 30.0%
TGATGAATAAGTCATGACAT+CGG + chr4_1:6243544-6243563 Msa0532230:intron 30.0%
CCACATCTACAAGTAAAATT+AGG - chr4_1:6243592-6243611 None:intergenic 30.0%
! CCTAATTTTACTTGTAGATG+TGG + chr4_1:6243589-6243608 Msa0532230:intron 30.0%
TTATCTCTAACAGGAAGATA+AGG - chr4_1:6243859-6243878 None:intergenic 30.0%
CATTACTCATTATCTCTAAC+AGG - chr4_1:6243868-6243887 None:intergenic 30.0%
TTTGAGCAACATATGGAAAT+AGG - chr4_1:6244076-6244095 None:intergenic 30.0%
!!! ACATTTATCTTTTCAAGTCC+TGG + chr4_1:6244095-6244114 Msa0532230:intron 30.0%
TGAAATCTTAAATGTTGCAC+AGG - chr4_1:6244137-6244156 None:intergenic 30.0%
! TTTCTCAATTTGGGATTACA+AGG - chr4_1:6244169-6244188 None:intergenic 30.0%
CAGAAAGGTAAGGAAAAAAT+TGG - chr4_1:6244303-6244322 None:intergenic 30.0%
TGATGTAATTACATGCAATG+GGG - chr4_1:6244505-6244524 None:intergenic 30.0%
GTTGATGTAATTACATGCAA+TGG - chr4_1:6244507-6244526 None:intergenic 30.0%
! TTACTGACTAACAGTTCATA+CGG - chr4_1:6244832-6244851 None:intergenic 30.0%
ATGTTCATACTTATTGCTTG+AGG - chr4_1:6245056-6245075 None:intergenic 30.0%
TAAGTATGAACATCATGAAG+GGG + chr4_1:6245063-6245082 Msa0532230:intron 30.0%
GAAATGGAGAGAATAAACAA+TGG - chr4_1:6245101-6245120 None:intergenic 30.0%
! AACTGTAGTAATGGAAGAAA+TGG - chr4_1:6245117-6245136 None:intergenic 30.0%
AACTGTGATTTCAAATCATG+TGG - chr4_1:6245190-6245209 None:intergenic 30.0%
GGTCTATAAAGAAACAACAA+AGG - chr4_1:6245716-6245735 None:intergenic 30.0%
! ATCAAGTAACTGTTGCTATT+TGG + chr4_1:6245868-6245887 Msa0532230:three_prime_UTR 30.0%
AAAACAGAAAACAGGGATGA+TGG - chr4_1:6242782-6242801 None:intergenic 35.0%
GATCAAGTATTCAACAAGGT+TGG + chr4_1:6242843-6242862 Msa0532230:CDS 35.0%
! TTGTTGGTAAGCAGATTCTA+TGG + chr4_1:6243157-6243176 Msa0532230:intron 35.0%
! CTTTTCAAAGTAGAAGCTCA+CGG + chr4_1:6243233-6243252 Msa0532230:intron 35.0%
GTACCAGTTCAACAAAAACA+AGG + chr4_1:6243285-6243304 Msa0532230:intron 35.0%
CAAGGAAAAATGAATACGTG+TGG + chr4_1:6243303-6243322 Msa0532230:intron 35.0%
TGTGGATGAGATATAGACAT+TGG + chr4_1:6243607-6243626 Msa0532230:intron 35.0%
AGTGATGCTGTATGAGAAAT+AGG + chr4_1:6243683-6243702 Msa0532230:intron 35.0%
GTTTGTAACAAAGCTAATGC+TGG - chr4_1:6243837-6243856 None:intergenic 35.0%
TCAAAACTGAACATGTGAGA+AGG - chr4_1:6243892-6243911 None:intergenic 35.0%
GACTGCATATCAAAAGAAGA+AGG - chr4_1:6243990-6244009 None:intergenic 35.0%
TACTTTGTTGACCAACCATT+TGG - chr4_1:6244039-6244058 None:intergenic 35.0%
! TGGTTGGTCAACAAAGTAAA+AGG + chr4_1:6244041-6244060 Msa0532230:CDS 35.0%
GAAAAAATTGGACATGAGAG+GGG - chr4_1:6244291-6244310 None:intergenic 35.0%
GGAAAAAATTGGACATGAGA+GGG - chr4_1:6244292-6244311 None:intergenic 35.0%
AGGAAAAAATTGGACATGAG+AGG - chr4_1:6244293-6244312 None:intergenic 35.0%
CTAACTCATCCCTACAAAAT+AGG - chr4_1:6244457-6244476 None:intergenic 35.0%
! CTATTTTGTAGGGATGAGTT+AGG + chr4_1:6244455-6244474 Msa0532230:intron 35.0%
TAATTACATGCAATGGGGAT+TGG - chr4_1:6244500-6244519 None:intergenic 35.0%
AACCATGTTTAGATGCTTTG+CGG + chr4_1:6244524-6244543 Msa0532230:intron 35.0%
TCGAAAGTCACCAATTACTA+AGG - chr4_1:6244568-6244587 None:intergenic 35.0%
! GTAATTGGTGACTTTCGAAA+CGG + chr4_1:6244570-6244589 Msa0532230:intron 35.0%
TATGTGCCCTAATCTTACAA+TGG + chr4_1:6244594-6244613 Msa0532230:intron 35.0%
GGAGATCCATTGTAAGATTA+GGG - chr4_1:6244603-6244622 None:intergenic 35.0%
TGGAGATCCATTGTAAGATT+AGG - chr4_1:6244604-6244623 None:intergenic 35.0%
TTTAGTACTTTGAACCATGG+AGG + chr4_1:6244657-6244676 Msa0532230:intron 35.0%
AACTTATAAACAGACCTCCA+TGG - chr4_1:6244674-6244693 None:intergenic 35.0%
AAATAAAGCAGAACCATTGG+TGG - chr4_1:6244996-6245015 None:intergenic 35.0%
GAGAAATAAAGCAGAACCAT+TGG - chr4_1:6244999-6245018 None:intergenic 35.0%
! ATTGCTTGAGGAAAACCTTT+TGG - chr4_1:6245044-6245063 None:intergenic 35.0%
GATTTCAAATCATGTGGCAA+AGG - chr4_1:6245184-6245203 None:intergenic 35.0%
TCCTTTAACAACTCTGGTAA+TGG - chr4_1:6245314-6245333 None:intergenic 35.0%
TCATACTCCTTTAACAACTC+TGG - chr4_1:6245320-6245339 None:intergenic 35.0%
! AAGGAGTATGATCTACCAAT+AGG + chr4_1:6245329-6245348 Msa0532230:CDS 35.0%
CTACGAATTTAACGAAGAGA+CGG + chr4_1:6245376-6245395 Msa0532230:CDS 35.0%
TACGAATTTAACGAAGAGAC+GGG + chr4_1:6245377-6245396 Msa0532230:CDS 35.0%
CATGAAGAAAACAAGGAGTA+GGG + chr4_1:6245598-6245617 Msa0532230:CDS 35.0%
GCATATGATGTTACAAGAGA+TGG + chr4_1:6245623-6245642 Msa0532230:CDS 35.0%
CCATACAAGAAGACTCAAAA+TGG - chr4_1:6245737-6245756 None:intergenic 35.0%
!! CCATTTTGAGTCTTCTTGTA+TGG + chr4_1:6245734-6245753 Msa0532230:three_prime_UTR 35.0%
TTTGAGTCTTCTTGTATGGT+TGG + chr4_1:6245738-6245757 Msa0532230:three_prime_UTR 35.0%
TTGAGTCTTCTTGTATGGTT+GGG + chr4_1:6245739-6245758 Msa0532230:three_prime_UTR 35.0%
!! GTATTTCAAGTACTGATCCT+TGG + chr4_1:6245789-6245808 Msa0532230:three_prime_UTR 35.0%
ATTTCTCAAATCACAACCCA+AGG - chr4_1:6245816-6245835 None:intergenic 35.0%
TATCAAGGATGTTCATGGTT+CGG - chr4_1:6245966-6245985 None:intergenic 35.0%
TCTAGTATCAAGGATGTTCA+TGG - chr4_1:6245971-6245990 None:intergenic 35.0%
TCAACAAGGTTGGATCCTAT+TGG + chr4_1:6242853-6242872 Msa0532230:CDS 40.0%
GGCTTTCTGATTGAACCAAT+AGG - chr4_1:6242871-6242890 None:intergenic 40.0%
AGCAAGGAGCTTAATTCTGT+CGG + chr4_1:6242891-6242910 Msa0532230:CDS 40.0%
!! TTTTAAGTACTAGACCACGG+TGG - chr4_1:6243192-6243211 None:intergenic 40.0%
! CGTGAGCTTCTACTTTGAAA+AGG - chr4_1:6243235-6243254 None:intergenic 40.0%
TTGTCTAACCGATAACGATC+AGG + chr4_1:6243362-6243381 Msa0532230:intron 40.0%
ACCGTTAACTCTTACTTCAG+AGG + chr4_1:6243414-6243433 Msa0532230:intron 40.0%
!! GCCTCTGAAGTAAGAGTTAA+CGG - chr4_1:6243418-6243437 None:intergenic 40.0%
!!! TTTTAAGTACTGGTCCACTG+TGG + chr4_1:6243470-6243489 Msa0532230:intron 40.0%
TGGTAAATGCAACACCAACA+GGG - chr4_1:6243512-6243531 None:intergenic 40.0%
AATAAGTCATGACATCGGTG+AGG + chr4_1:6243549-6243568 Msa0532230:intron 40.0%
ATAAGTCATGACATCGGTGA+GGG + chr4_1:6243550-6243569 Msa0532230:intron 40.0%
AGATGATTCTGTTACGGTGA+GGG + chr4_1:6243657-6243676 Msa0532230:intron 40.0%
GCTAATGCTGGATTATGACA+CGG - chr4_1:6243825-6243844 None:intergenic 40.0%
GGATCAAAATACTCGCAGTT+AGG + chr4_1:6244202-6244221 Msa0532230:intron 40.0%
! TTTTTCTGTGCATCAGCGTA+GGG + chr4_1:6244242-6244261 Msa0532230:intron 40.0%
TCAATGCGAGACTCAACAAA+GGG - chr4_1:6244378-6244397 None:intergenic 40.0%
TTCAATGCGAGACTCAACAA+AGG - chr4_1:6244379-6244398 None:intergenic 40.0%
! AACCGCAAAGCATCTAAACA+TGG - chr4_1:6244529-6244548 None:intergenic 40.0%
CTAAACCAGTTTGACACTAG+TGG + chr4_1:6244958-6244977 Msa0532230:intron 40.0%
TCATACCACTAGTGTCAAAC+TGG - chr4_1:6244966-6244985 None:intergenic 40.0%
ATGAACATCATGAAGGGGTT+AGG + chr4_1:6245068-6245087 Msa0532230:intron 40.0%
GGAGAGAATAAACAATGGAG+AGG - chr4_1:6245096-6245115 None:intergenic 40.0%
GCCATTACCAGAGTTGTTAA+AGG + chr4_1:6245310-6245329 Msa0532230:CDS 40.0%
TCACGAGGAAAGATTCCTAT+TGG - chr4_1:6245347-6245366 None:intergenic 40.0%
! GTTACTGGTTATCTGGAGAA+AGG + chr4_1:6245476-6245495 Msa0532230:CDS 40.0%
GCATGAAGAAAACAAGGAGT+AGG + chr4_1:6245597-6245616 Msa0532230:CDS 40.0%
GGGTTGTGATTTGAGAAATG+TGG + chr4_1:6245817-6245836 Msa0532230:three_prime_UTR 40.0%
GCTAAACGTGATACTCAACA+CGG - chr4_1:6245938-6245957 None:intergenic 40.0%
ATCAAGGATGTTCATGGTTC+GGG - chr4_1:6245965-6245984 None:intergenic 40.0%
AAGTTGGCTGCTTATTCGCT+AGG - chr4_1:6242745-6242764 None:intergenic 45.0%
TAGCGAATAAGCAGCCAACT+TGG + chr4_1:6242744-6242763 Msa0532230:five_prime_UTR 45.0%
GGACAACAACGATACCAAGT+TGG - chr4_1:6242761-6242780 None:intergenic 45.0%
GTTCAATCAGAAAGCCAGCA+AGG + chr4_1:6242875-6242894 Msa0532230:CDS 45.0%
GACAGAATTAAGCTCCTTGC+TGG - chr4_1:6242892-6242911 None:intergenic 45.0%
GCGTGTTTCCAATTTCCATG+AGG - chr4_1:6243022-6243041 None:intergenic 45.0%
GACAGTGACCTGATCGTTAT+CGG - chr4_1:6243373-6243392 None:intergenic 45.0%
! TCTTAGCCATGATGACGAGT+AGG + chr4_1:6243391-6243410 Msa0532230:intron 45.0%
TAAATGCAACACCAACAGGG+TGG - chr4_1:6243509-6243528 None:intergenic 45.0%
CTGGTAAATGCAACACCAAC+AGG - chr4_1:6243513-6243532 None:intergenic 45.0%
TAAGTCATGACATCGGTGAG+GGG + chr4_1:6243551-6243570 Msa0532230:intron 45.0%
GAGATGATTCTGTTACGGTG+AGG + chr4_1:6243656-6243675 Msa0532230:intron 45.0%
ATGCAGTTTGAGTGCGTTGT+AGG + chr4_1:6243736-6243755 Msa0532230:intron 45.0%
! TCACTGTCGAACAGTATCGA+AGG + chr4_1:6244008-6244027 Msa0532230:CDS 45.0%
! GTTTTTCTGTGCATCAGCGT+AGG + chr4_1:6244241-6244260 Msa0532230:intron 45.0%
ATGAGAGGGGTGAGAATACA+GGG - chr4_1:6244278-6244297 None:intergenic 45.0%
CGAGAGAAGACAGAAAGGTA+AGG - chr4_1:6244313-6244332 None:intergenic 45.0%
AGAGACGAGAGAAGACAGAA+AGG - chr4_1:6244318-6244337 None:intergenic 45.0%
TACATGCAATGGGGATTGGA+AGG - chr4_1:6244496-6244515 None:intergenic 45.0%
ATGGATCTCCACAGTTAGTG+TGG + chr4_1:6244613-6244632 Msa0532230:intron 45.0%
GTATGAAGCCAAACCACCAA+TGG + chr4_1:6244980-6244999 Msa0532230:intron 45.0%
! AAGCAGAACCATTGGTGGTT+TGG - chr4_1:6244991-6245010 None:intergenic 45.0%
!! TCGTAGTTTGTTGCATCACG+AGG - chr4_1:6245362-6245381 None:intergenic 45.0%
GCTTGAAGTCTTCATTCCTC+AGG + chr4_1:6245403-6245422 Msa0532230:CDS 45.0%
AGTGGTGAAGAAACGCAAGA+CGG - chr4_1:6245456-6245475 None:intergenic 45.0%
CGTTTCTTCACCACTGTTAC+TGG + chr4_1:6245461-6245480 Msa0532230:CDS 45.0%
! CACCACTGTTACTGGTTATC+TGG + chr4_1:6245469-6245488 Msa0532230:CDS 45.0%
CTCCAGATAACCAGTAACAG+TGG - chr4_1:6245474-6245493 None:intergenic 45.0%
AGGAAAGCTAGCAGACATAG+AGG + chr4_1:6245496-6245515 Msa0532230:CDS 45.0%
GGAAAGCTAGCAGACATAGA+GGG + chr4_1:6245497-6245516 Msa0532230:CDS 45.0%
! AGTGACAACCATTTTCTCCG+AGG + chr4_1:6245550-6245569 Msa0532230:CDS 45.0%
! GTGACAACCATTTTCTCCGA+GGG + chr4_1:6245551-6245570 Msa0532230:CDS 45.0%
CTGCTGGCATGAAGAAAACA+AGG + chr4_1:6245591-6245610 Msa0532230:CDS 45.0%
AGTCTTCTTGTATGGTTGGG+TGG + chr4_1:6245742-6245761 Msa0532230:three_prime_UTR 45.0%
!! AAGTACTGATCCTTGGACCT+TGG + chr4_1:6245796-6245815 Msa0532230:three_prime_UTR 45.0%
!! AGTACTGATCCTTGGACCTT+GGG + chr4_1:6245797-6245816 Msa0532230:three_prime_UTR 45.0%
AAATCACAACCCAAGGTCCA+AGG - chr4_1:6245809-6245828 None:intergenic 45.0%
! TACTCAACACGGTTTGGGTT+TGG - chr4_1:6245927-6245946 None:intergenic 45.0%
CGTGATACTCAACACGGTTT+GGG - chr4_1:6245932-6245951 None:intergenic 45.0%
ACGTGATACTCAACACGGTT+TGG - chr4_1:6245933-6245952 None:intergenic 45.0%
ATCAAGTCACCGTCCTAGCT+AGG - chr4_1:6243263-6243282 None:intergenic 50.0%
AGCTAGGACGGTGACTTGAT+CGG + chr4_1:6243263-6243282 Msa0532230:intron 50.0%
AACGGTCCTACTCGTCATCA+TGG - chr4_1:6243400-6243419 None:intergenic 50.0%
GATTCTATGGTGGTCCACAG+TGG - chr4_1:6243487-6243506 None:intergenic 50.0%
! CAACAGGGTGGATTCTATGG+TGG - chr4_1:6243497-6243516 None:intergenic 50.0%
CACCATAGAATCCACCCTGT+TGG + chr4_1:6243495-6243514 Msa0532230:intron 50.0%
! CACCAACAGGGTGGATTCTA+TGG - chr4_1:6243500-6243519 None:intergenic 50.0%
TTAAGCAAGGGAAAGACGCG+AGG + chr4_1:6243774-6243793 Msa0532230:intron 50.0%
! CTGTCGAACAGTATCGAAGG+CGG + chr4_1:6244011-6244030 Msa0532230:CDS 50.0%
GTATCGAAGGCGGAACCAAA+TGG + chr4_1:6244021-6244040 Msa0532230:CDS 50.0%
!!! CTTTTCAAGTCCTGGACTCC+TGG + chr4_1:6244103-6244122 Msa0532230:intron 50.0%
GTTGCACAGGTTTCATAGCC+AGG - chr4_1:6244124-6244143 None:intergenic 50.0%
CATGAGAGGGGTGAGAATAC+AGG - chr4_1:6244279-6244298 None:intergenic 50.0%
TGCTCAGTCCACACTAACTG+TGG - chr4_1:6244624-6244643 None:intergenic 50.0%
ATTCCTCAGGTCTGCGAAGT+AGG + chr4_1:6245416-6245435 Msa0532230:CDS 50.0%
GGTCTGCGAAGTAGGCTATA+AGG + chr4_1:6245424-6245443 Msa0532230:CDS 50.0%
CTTCGATCCCTCGGAGAAAA+TGG - chr4_1:6245561-6245580 None:intergenic 50.0%
CACATAGAGCTTCGATCCCT+CGG - chr4_1:6245570-6245589 None:intergenic 50.0%
TCGAAGCTCTATGTGACTGC+TGG + chr4_1:6245575-6245594 Msa0532230:CDS 50.0%
! GGATGTTCATGGTTCGGGTT+TGG - chr4_1:6245960-6245979 None:intergenic 50.0%
TATGGTCACGTTGTCCACCG+TGG + chr4_1:6243175-6243194 Msa0532230:intron 55.0%
AAGTCACCGTCCTAGCTAGG+AGG - chr4_1:6243260-6243279 None:intergenic 55.0%
GACATCGGTGAGGGGAAATG+TGG + chr4_1:6243559-6243578 Msa0532230:intron 55.0%
GACGCGAGGATCTGCAACTT+AGG + chr4_1:6243788-6243807 Msa0532230:intron 55.0%
CGAAGGCGGAACCAAATGGT+TGG + chr4_1:6244025-6244044 Msa0532230:CDS 55.0%
GGTTTCATAGCCAGGAGTCC+AGG - chr4_1:6244116-6244135 None:intergenic 55.0%
TAGCCTACTTCGCAGACCTG+AGG - chr4_1:6245422-6245441 None:intergenic 55.0%
AGCTCACGGTCCTCCTAGCT+AGG + chr4_1:6243247-6243266 Msa0532230:intron 60.0%
CACGGTCCTCCTAGCTAGGA+CGG + chr4_1:6243251-6243270 Msa0532230:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 6242727 6246023 6242727 ID=Msa0532230;Name=Msa0532230
chr4_1 mRNA 6242727 6246023 6242727 ID=Msa0532230-mRNA-1;Parent=Msa0532230;Name=Msa0532230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=113|1|1|1|1|1|3|356|170
chr4_1 exon 6242727 6242926 6242727 ID=Msa0532230-mRNA-1:exon:2218;Parent=Msa0532230-mRNA-1
chr4_1 exon 6244008 6244062 6244008 ID=Msa0532230-mRNA-1:exon:2219;Parent=Msa0532230-mRNA-1
chr4_1 exon 6245297 6246023 6245297 ID=Msa0532230-mRNA-1:exon:2220;Parent=Msa0532230-mRNA-1
chr4_1 five_prime_UTR 6242727 6242839 6242727 ID=Msa0532230-mRNA-1:five_prime_utr;Parent=Msa0532230-mRNA-1
chr4_1 CDS 6242840 6242926 6242840 ID=Msa0532230-mRNA-1:cds;Parent=Msa0532230-mRNA-1
chr4_1 CDS 6244008 6244062 6244008 ID=Msa0532230-mRNA-1:cds;Parent=Msa0532230-mRNA-1
chr4_1 CDS 6245297 6245667 6245297 ID=Msa0532230-mRNA-1:cds;Parent=Msa0532230-mRNA-1
chr4_1 three_prime_UTR 6245668 6246023 6245668 ID=Msa0532230-mRNA-1:three_prime_utr;Parent=Msa0532230-mRNA-1
Gene Sequence

>Msa0532230

ATGGATCAAGTATTCAACAAGGTTGGATCCTATTGGTTCAATCAGAAAGCCAGCAAGGAGCTTAATTCTGTCGGTGATGAAATCAATTCACTGTCGAACAGTATCGAAGGCGGAACCAAATGGTTGGTCAACAAAGTAAAAGGAAAAATGCAGAAGCCATTACCAGAGTTGTTAAAGGAGTATGATCTACCAATAGGAATCTTTCCTCGTGATGCAACAAACTACGAATTTAACGAAGAGACGGGAAAGCTTGAAGTCTTCATTCCTCAGGTCTGCGAAGTAGGCTATAAGGATTCATCCGTCTTGCGTTTCTTCACCACTGTTACTGGTTATCTGGAGAAAGGAAAGCTAGCAGACATAGAGGGAATGAAGACAAAAGTTATTATCTGGGTAAAAGTGACAACCATTTTCTCCGAGGGATCGAAGCTCTATGTGACTGCTGGCATGAAGAAAACAAGGAGTAGGGAAGCATATGATGTTACAAGAGATGGCGTACCTGTAGATAAGTTCTAA
Protein sequence

>Msa0532230

MDQVFNKVGSYWFNQKASKELNSVGDEINSLSNSIEGGTKWLVNKVKGKMQKPLPELLKEYDLPIGIFPRDATNYEFNEETGKLEVFIPQVCEVGYKDSSVLRFFTTVTGYLEKGKLADIEGMKTKVIIWVKVTTIFSEGSKLYVTAGMKKTRSREAYDVTRDGVPVDKF*