AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0766310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0766310 MtrunA17_Chr5g0417481 93.976 166 10 0 1 166 1 166 2.23e-115 323
Msa0766310 MtrunA17_Chr2g0302051 34.711 121 75 2 46 163 45 164 2.61e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0766310 AT1G14205.1 61.728 162 61 1 5 165 8 169 6.50e-68 204
Msa0766310 AT1G48350.1 34.127 126 79 2 41 163 44 168 3.81e-18 77.4

Find 86 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTAACTCATCAAAATT+AGG 0.150461 5_2:+27946698 None:intergenic
AAGCTACATTCACACATTTA+TGG 0.170943 5_2:+27946665 None:intergenic
AGAATAAGCACTTCTTATTT+TGG 0.196854 5_2:+27947588 None:intergenic
GAGATTGCTATTTCCAATTA+TGG 0.208902 5_2:-27947278 Msa0766310:CDS
AAAGGGTGTTTAATTATATT+TGG 0.229075 5_2:-27946309 Msa0766310:three_prime_UTR
GGGAGAGTGTGATATGATTA+TGG 0.253558 5_2:-27946344 Msa0766310:three_prime_UTR
CGCAACCAGTGTGTTGGTTC+TGG 0.265562 5_2:+27948921 None:intergenic
TTATGGGAGATGGAGTTAAA+AGG 0.276006 5_2:-27946327 Msa0766310:three_prime_UTR
TCCTATGATCGCAATGGTTT+TGG 0.281803 5_2:-27947326 Msa0766310:CDS
TGAAAGGAAATGAATGGTTT+TGG 0.296469 5_2:+27948814 None:intergenic
AATCACTTCACCTCAGGCTT+TGG 0.302760 5_2:+27946541 None:intergenic
AATAAGAAGTGCTTATTCTA+TGG 0.305092 5_2:-27947584 Msa0766310:CDS
TCTAGGCAAGAATCCATAAT+TGG 0.308302 5_2:+27947265 None:intergenic
AAACAGAAAGATGCAGAAAA+AGG 0.318727 5_2:-27948364 Msa0766310:intron
GAATTAAGAGTCTTAAAATA+TGG 0.323497 5_2:-27948899 Msa0766310:exon
ATCACTTCACCTCAGGCTTT+GGG 0.329702 5_2:+27946542 None:intergenic
GGTTTAACTCATCAAAATTA+GGG 0.340547 5_2:+27946699 None:intergenic
TTCAATACTTCTTCTTTCTT+CGG 0.345225 5_2:-27948844 Msa0766310:CDS
TGAAGGTCTTGGCGTTCTCT+TGG 0.347741 5_2:-27946270 Msa0766310:three_prime_UTR
CTTAAAACATTATATTCCTT+TGG 0.358990 5_2:+27946724 None:intergenic
TTATGGATTCTTGCCTAGAT+AGG 0.359313 5_2:-27947261 Msa0766310:intron
AATAACTTACTTATACTTTG+TGG 0.391351 5_2:+27948790 None:intergenic
GTCTTAAAATATGGTTCTAA+TGG 0.394319 5_2:-27948890 Msa0766310:exon
AGTGTGTTGGTTCTGGAGAT+TGG 0.413895 5_2:+27948928 None:intergenic
CCCGCGTCGGAATACGGTCT+TGG 0.427808 5_2:+27946239 None:intergenic
TAATTATATTTGGAAGAGTC+CGG 0.443365 5_2:-27946299 Msa0766310:three_prime_UTR
TCATGCAATCACTTCACCTC+AGG 0.444320 5_2:+27946535 None:intergenic
AGTTTATAACACATGGGTAA+TGG 0.444531 5_2:-27946422 Msa0766310:three_prime_UTR
GATTTACCCGCGTCGGAATA+CGG 0.444762 5_2:+27946233 None:intergenic
GGAGAGTGTGATATGATTAT+GGG 0.444880 5_2:-27946343 Msa0766310:three_prime_UTR
ACCTCTTTACTTGAAAGGTT+GGG 0.449065 5_2:-27946364 Msa0766310:three_prime_UTR
TATGGGAGATGGAGTTAAAA+GGG 0.451819 5_2:-27946326 Msa0766310:three_prime_UTR
ACCAAAACCATTGCGATCAT+AGG 0.452925 5_2:+27947325 None:intergenic
GACAGACCTCTTTACTTGAA+AGG 0.458873 5_2:-27946369 Msa0766310:three_prime_UTR
GACCTCTTTACTTGAAAGGT+TGG 0.460294 5_2:-27946365 Msa0766310:three_prime_UTR
AGGAAGCTGCTCAACGTGTA+GGG 0.461921 5_2:-27947397 Msa0766310:CDS
TATCAGTTACATACCTATCT+AGG 0.463186 5_2:+27947248 None:intergenic
ACTACTTACTGCAGTGCTAC+AGG 0.464722 5_2:+27947526 None:intergenic
TTCTTGGCTAGGTACTTCCT+AGG 0.464798 5_2:-27946771 Msa0766310:intron
TTTAGTTAAGAAACCGTTAG+TGG 0.466736 5_2:-27948430 Msa0766310:CDS
AACGCAAGCGTAGTTGGTCG+TGG 0.466857 5_2:-27949005 Msa0766310:five_prime_UTR
ATCTTCAATTAGAAGACCCT+GGG 0.476475 5_2:+27946505 None:intergenic
CTGTATAAGTTTATAACACA+TGG 0.477959 5_2:-27946429 Msa0766310:three_prime_UTR
TCAGCCCGCGAGTCAACATC+TGG 0.484432 5_2:+27946476 None:intergenic
ACTTATACTTTGTGGTTGAA+AGG 0.484617 5_2:+27948798 None:intergenic
TCGGGAAACGCAAGCGTAGT+TGG 0.494457 5_2:-27949011 Msa0766310:five_prime_UTR
CGTAGTTGGTCGTGGGTCGC+CGG 0.503377 5_2:-27948997 Msa0766310:five_prime_UTR
ACATTTATGGATTCATCGAA+TGG 0.508129 5_2:+27946678 None:intergenic
GAGCAAAATACTGAAAGCCT+TGG 0.512963 5_2:+27947644 None:intergenic
TGTGGTTGAAAGGAAATGAA+TGG 0.515986 5_2:+27948808 None:intergenic
TCCCAACCTTTCAAGTAAAG+AGG 0.522822 5_2:+27946363 None:intergenic
TAGGAAGCTGCTCAACGTGT+AGG 0.524121 5_2:-27947398 Msa0766310:CDS
CATCTTCAATTAGAAGACCC+TGG 0.526450 5_2:+27946504 None:intergenic
TGTGATATGATTATGGGAGA+TGG 0.534042 5_2:-27946337 Msa0766310:three_prime_UTR
GCCTTGGCTTTAGCCACTAA+CGG 0.538041 5_2:+27948417 None:intergenic
AGAGAACGCCAAGACCTTCA+AGG 0.541606 5_2:+27946273 None:intergenic
GAGAACGCCAAGACCTTCAA+GGG 0.543756 5_2:+27946274 None:intergenic
GCGTCGGAATACGGTCTTGG+AGG 0.548137 5_2:+27946242 None:intergenic
CTACTTACTGCAGTGCTACA+GGG 0.557559 5_2:+27947527 None:intergenic
GATTGCATGAAGAAATGCCC+AGG 0.560342 5_2:-27946522 Msa0766310:three_prime_UTR
ACCGTTAGTGGCTAAAGCCA+AGG 0.574909 5_2:-27948418 Msa0766310:CDS
TTAATTCGCAACCAGTGTGT+TGG 0.582012 5_2:+27948915 None:intergenic
CTCTCGAGTCTAAACTCCAA+AGG 0.582764 5_2:-27946740 Msa0766310:three_prime_UTR
ATGGTACTGCAACACATCCA+AGG 0.592302 5_2:-27947661 Msa0766310:CDS
AAACTTATACAGACCATGGA+TGG 0.593278 5_2:+27946440 None:intergenic
ACTCGAGAGTTCTCGCACCT+AGG 0.594844 5_2:+27946754 None:intergenic
ATTTATGTTTAGATCAACAC+AGG 0.599501 5_2:+27947358 None:intergenic
AAAACCAGATGTTGACTCGC+GGG 0.600523 5_2:-27946480 Msa0766310:three_prime_UTR
TGCAAAACTAGCACCATCCA+TGG 0.601140 5_2:-27946453 Msa0766310:three_prime_UTR
TTATAAACTTATACAGACCA+TGG 0.609104 5_2:+27946436 None:intergenic
GTGAATGTAGCTTCATCAGT+TGG 0.610439 5_2:-27946655 Msa0766310:three_prime_UTR
ACGCAAGCGTAGTTGGTCGT+GGG 0.613877 5_2:-27949004 Msa0766310:five_prime_UTR
ATTGGAAATAGCAATCTCAA+AGG 0.616550 5_2:+27947283 None:intergenic
CCAAGACCGTATTCCGACGC+GGG 0.616950 5_2:-27946239 Msa0766310:three_prime_UTR
AGCTGCTCAACGTGTAGGGG+AGG 0.617938 5_2:-27947393 Msa0766310:CDS
GGAAGCTGCTCAACGTGTAG+GGG 0.626803 5_2:-27947396 Msa0766310:CDS
ATATCATCCTATGATCGCAA+TGG 0.637436 5_2:-27947332 Msa0766310:CDS
AAAGCAAGATTTACCCGCGT+CGG 0.647193 5_2:+27946226 None:intergenic
AATCTCCAGAACCAACACAC+TGG 0.647386 5_2:-27948926 Msa0766310:five_prime_UTR
TGTATAAGTTTATAACACAT+GGG 0.663095 5_2:-27946428 Msa0766310:three_prime_UTR
TCCAAGACCGTATTCCGACG+CGG 0.671398 5_2:-27946240 Msa0766310:three_prime_UTR
GATAAAACTCCCAAAGCCTG+AGG 0.687641 5_2:-27946551 Msa0766310:three_prime_UTR
ATTGCATGAAGAAATGCCCA+GGG 0.691999 5_2:-27946521 Msa0766310:three_prime_UTR
AGAACGCCAAGACCTTCAAG+GGG 0.700632 5_2:+27946275 None:intergenic
CTTACTGCAGTGCTACAGGG+AGG 0.759661 5_2:+27947530 None:intergenic
GAACGCCAAGACCTTCAAGG+GGG 0.762989 5_2:+27946276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTATGATATTATCTTA+AGG + chr5_2:27948272-27948291 None:intergenic 10.0%
!!! ATTCATCATAATATCAATTT+TGG - chr5_2:27946780-27946799 Msa0766310:intron 15.0%
!!! ATATTGTATAGTTTTTCTTT+TGG - chr5_2:27946965-27946984 Msa0766310:intron 15.0%
!! ACCATTAAACTAAAAAAAAT+GGG + chr5_2:27947616-27947635 None:intergenic 15.0%
!! TACCATTAAACTAAAAAAAA+TGG + chr5_2:27947617-27947636 None:intergenic 15.0%
!!! TACATGTTAAAATGTTTTAA+TGG - chr5_2:27948373-27948392 Msa0766310:CDS 15.0%
!! GAATTAAGAGTCTTAAAATA+TGG - chr5_2:27946393-27946412 Msa0766310:three_prime_UTR 20.0%
!! AATAACTTACTTATACTTTG+TGG + chr5_2:27946505-27946524 None:intergenic 20.0%
!!! CATGCTTTTCATATTTTATA+AGG - chr5_2:27946583-27946602 Msa0766310:three_prime_UTR 20.0%
!! TTATAAGGTCTATAATTGAT+TGG - chr5_2:27946598-27946617 Msa0766310:three_prime_UTR 20.0%
!! ACATAATTCAATGAAATCAT+AGG - chr5_2:27946694-27946713 Msa0766310:three_prime_UTR 20.0%
!! TGAAATTTGAATTTACTTTC+AGG - chr5_2:27946839-27946858 Msa0766310:intron 20.0%
!!! GATTTTGATAGAATTGATTT+TGG - chr5_2:27947200-27947219 Msa0766310:intron 20.0%
!! ATGGTATTAATAAAAGATAG+AGG + chr5_2:27947584-27947603 None:intergenic 20.0%
!!! TGAAAATTTGCTTAATTAGA+AGG - chr5_2:27948293-27948312 Msa0766310:intron 20.0%
!! ATATGTTACTAACTTAATGA+TGG - chr5_2:27948344-27948363 Msa0766310:intron 20.0%
!! TTATTACATGTCATGATAAT+TGG - chr5_2:27948461-27948480 Msa0766310:intron 20.0%
!! TATTACATGTCATGATAATT+GGG - chr5_2:27948462-27948481 Msa0766310:intron 20.0%
!! CTTAAAACATTATATTCCTT+TGG + chr5_2:27948571-27948590 None:intergenic 20.0%
!!! CAAAGGAATATAATGTTTTA+AGG - chr5_2:27948569-27948588 Msa0766310:intron 20.0%
!! TGTATAAGTTTATAACACAT+GGG - chr5_2:27948864-27948883 Msa0766310:CDS 20.0%
!!! AAAGGGTGTTTAATTATATT+TGG - chr5_2:27948983-27949002 Msa0766310:five_prime_UTR 20.0%
!!! GTCTTAAAATATGGTTCTAA+TGG - chr5_2:27946402-27946421 Msa0766310:three_prime_UTR 25.0%
! TTCAATACTTCTTCTTTCTT+CGG - chr5_2:27946448-27946467 Msa0766310:three_prime_UTR 25.0%
!! AAATGGATTTAGTGTATGTT+TGG + chr5_2:27947439-27947458 None:intergenic 25.0%
! ATTGTTTAAATATGCTACAG+TGG - chr5_2:27947487-27947506 Msa0766310:intron 25.0%
! TTAAACTAAAAAAAATGGGC+AGG + chr5_2:27947612-27947631 None:intergenic 25.0%
!!! GCCCATTTTTTTTAGTTTAA+TGG - chr5_2:27947612-27947631 Msa0766310:CDS 25.0%
!!! AGAATAAGCACTTCTTATTT+TGG + chr5_2:27947707-27947726 None:intergenic 25.0%
!!! AATAAGAAGTGCTTATTCTA+TGG - chr5_2:27947708-27947727 Msa0766310:intron 25.0%
!!! CAATATCTGTCTTTAAAATG+TGG + chr5_2:27947795-27947814 None:intergenic 25.0%
!!! TTTGGATTTTTTCTTATGCT+TGG - chr5_2:27947857-27947876 Msa0766310:intron 25.0%
!!! TTGGATTTTTTCTTATGCTT+GGG - chr5_2:27947858-27947877 Msa0766310:intron 25.0%
! ATTTATGTTTAGATCAACAC+AGG + chr5_2:27947937-27947956 None:intergenic 25.0%
! AAGCATTATGATGTTCTTAT+TGG - chr5_2:27948094-27948113 Msa0766310:intron 25.0%
! AGCATTATGATGTTCTTATT+GGG - chr5_2:27948095-27948114 Msa0766310:intron 25.0%
! ATTAGAACTTTGAATAATCC+AGG - chr5_2:27948172-27948191 Msa0766310:intron 25.0%
!! AAATTTGCTTAATTAGAAGG+AGG - chr5_2:27948296-27948315 Msa0766310:intron 25.0%
! ATTACATGTCATGATAATTG+GGG - chr5_2:27948463-27948482 Msa0766310:intron 25.0%
! GGTTTAACTCATCAAAATTA+GGG + chr5_2:27948596-27948615 None:intergenic 25.0%
! TGGTTTAACTCATCAAAATT+AGG + chr5_2:27948597-27948616 None:intergenic 25.0%
! TTATAAACTTATACAGACCA+TGG + chr5_2:27948859-27948878 None:intergenic 25.0%
! CTGTATAAGTTTATAACACA+TGG - chr5_2:27948863-27948882 Msa0766310:CDS 25.0%
! TAATTATATTTGGAAGAGTC+CGG - chr5_2:27948993-27949012 Msa0766310:five_prime_UTR 25.0%
! AATGATTTTCTCACTGTTAC+CGG + chr5_2:27946317-27946336 None:intergenic 30.0%
! GAAAGGAAATGAATGGTTTT+GGG + chr5_2:27946480-27946499 None:intergenic 30.0%
! TGAAAGGAAATGAATGGTTT+TGG + chr5_2:27946481-27946500 None:intergenic 30.0%
ACTTATACTTTGTGGTTGAA+AGG + chr5_2:27946497-27946516 None:intergenic 30.0%
GAAATCATAGGTATTAGTGT+CGG - chr5_2:27946706-27946725 Msa0766310:three_prime_UTR 30.0%
TTTAGTTAAGAAACCGTTAG+TGG - chr5_2:27946862-27946881 Msa0766310:intron 30.0%
AAACAGAAAGATGCAGAAAA+AGG - chr5_2:27946928-27946947 Msa0766310:intron 30.0%
! GACTAAAAGTGATTTGTGTT+TGG - chr5_2:27947237-27947256 Msa0766310:intron 30.0%
! AGAATTGATTCTGACATGTT+TGG + chr5_2:27947397-27947416 None:intergenic 30.0%
AAACATGTCAGAATCAATTC+TGG - chr5_2:27947396-27947415 Msa0766310:CDS 30.0%
!! TATCTGTCTTTAAAATGTGG+TGG + chr5_2:27947792-27947811 None:intergenic 30.0%
! GTTACATTAGACTTTAGCTT+TGG - chr5_2:27947839-27947858 Msa0766310:intron 30.0%
! CTTGGGTGAAATATTGTTTT+AGG - chr5_2:27947875-27947894 Msa0766310:intron 30.0%
ATTGGAAATAGCAATCTCAA+AGG + chr5_2:27948012-27948031 None:intergenic 30.0%
!! GAGATTGCTATTTCCAATTA+TGG - chr5_2:27948014-27948033 Msa0766310:intron 30.0%
TATCAGTTACATACCTATCT+AGG + chr5_2:27948047-27948066 None:intergenic 30.0%
GACTTGATGAGTAAGAAAAT+GGG - chr5_2:27948209-27948228 Msa0766310:intron 30.0%
GTTACTAACTTAATGATGGT+AGG - chr5_2:27948348-27948367 Msa0766310:intron 30.0%
!!! TGTAATTTCCCTTGTTTTCT+TGG - chr5_2:27948505-27948524 Msa0766310:intron 30.0%
ACATTTATGGATTCATCGAA+TGG + chr5_2:27948617-27948636 None:intergenic 30.0%
AAGCTACATTCACACATTTA+TGG + chr5_2:27948630-27948649 None:intergenic 30.0%
AGTTTATAACACATGGGTAA+TGG - chr5_2:27948870-27948889 Msa0766310:CDS 30.0%
!!! CGATAGTTTCGTTGTTTTGA+AGG + chr5_2:27946259-27946278 None:intergenic 35.0%
AAACAACGAAACTATCGCAT+CGG - chr5_2:27946262-27946281 Msa0766310:three_prime_UTR 35.0%
TGTGGTTGAAAGGAAATGAA+TGG + chr5_2:27946487-27946506 None:intergenic 35.0%
TCACATATACTCCTCGAATA+AGG + chr5_2:27946761-27946780 None:intergenic 35.0%
! TTCTTTTCTTGCATTTGCCT+TGG + chr5_2:27946894-27946913 None:intergenic 35.0%
!! CTTTTGGTTGTTAAATGTGC+TGG - chr5_2:27946981-27947000 Msa0766310:intron 35.0%
! TGTTAAATGTGCTGGTGTAA+TGG - chr5_2:27946989-27947008 Msa0766310:intron 35.0%
TTCTAACTGAAATGCGTGTT+TGG + chr5_2:27947044-27947063 None:intergenic 35.0%
TTTCCTTAGTTAGCAGTAGT+TGG - chr5_2:27947119-27947138 Msa0766310:intron 35.0%
AAACCAACTACTGCTAACTA+AGG + chr5_2:27947125-27947144 None:intergenic 35.0%
AACTAAGTGTGTGTTTCGTT+AGG - chr5_2:27947163-27947182 Msa0766310:intron 35.0%
! AATTGATTCTCGTTGAAGCT+AGG + chr5_2:27947337-27947356 None:intergenic 35.0%
AAAAAATGGGCAGGATAACA+TGG + chr5_2:27947603-27947622 None:intergenic 35.0%
AGACAGATATTGTATGAGAC+TGG - chr5_2:27947803-27947822 Msa0766310:intron 35.0%
GACAGATATTGTATGAGACT+GGG - chr5_2:27947804-27947823 Msa0766310:intron 35.0%
ATATCATCCTATGATCGCAA+TGG - chr5_2:27947960-27947979 Msa0766310:intron 35.0%
TCTAGGCAAGAATCCATAAT+TGG + chr5_2:27948030-27948049 None:intergenic 35.0%
TTATGGATTCTTGCCTAGAT+AGG - chr5_2:27948031-27948050 Msa0766310:intron 35.0%
AATCCAGGCATCAAATGTTA+TGG - chr5_2:27948187-27948206 Msa0766310:intron 35.0%
GGACTTGATGAGTAAGAAAA+TGG - chr5_2:27948208-27948227 Msa0766310:intron 35.0%
TGATGAGTAAGAAAATGGGA+TGG - chr5_2:27948213-27948232 Msa0766310:intron 35.0%
GATAATTGGGGTAAAGTTGA+AGG - chr5_2:27948475-27948494 Msa0766310:intron 35.0%
!!! GCTTTGGGAGTTTTATCAAA+TGG + chr5_2:27948738-27948757 None:intergenic 35.0%
ATCTTCAATTAGAAGACCCT+GGG + chr5_2:27948790-27948809 None:intergenic 35.0%
AAACTTATACAGACCATGGA+TGG + chr5_2:27948855-27948874 None:intergenic 35.0%
ACCTCTTTACTTGAAAGGTT+GGG - chr5_2:27948928-27948947 Msa0766310:five_prime_UTR 35.0%
! GGAGAGTGTGATATGATTAT+GGG - chr5_2:27948949-27948968 Msa0766310:five_prime_UTR 35.0%
TGTGATATGATTATGGGAGA+TGG - chr5_2:27948955-27948974 Msa0766310:five_prime_UTR 35.0%
TTATGGGAGATGGAGTTAAA+AGG - chr5_2:27948965-27948984 Msa0766310:five_prime_UTR 35.0%
TATGGGAGATGGAGTTAAAA+GGG - chr5_2:27948966-27948985 Msa0766310:five_prime_UTR 35.0%
AACAACGAAACTATCGCATC+GGG - chr5_2:27946263-27946282 Msa0766310:three_prime_UTR 40.0%
TTAATTCGCAACCAGTGTGT+TGG + chr5_2:27946380-27946399 None:intergenic 40.0%
TAGAAGTTTCAGTGCTGCAT+AGG - chr5_2:27947059-27947078 Msa0766310:intron 40.0%
GCTTCAACGAGAATCAATTC+TGG - chr5_2:27947338-27947357 Msa0766310:CDS 40.0%
TCAACGAGAATCAATTCTGG+AGG - chr5_2:27947341-27947360 Msa0766310:CDS 40.0%
! AATTCTGGTCTCTCTAGAAG+TGG - chr5_2:27947411-27947430 Msa0766310:intron 40.0%
GAGCAAAATACTGAAAGCCT+TGG + chr5_2:27947651-27947670 None:intergenic 40.0%
! TCCTATGATCGCAATGGTTT+TGG - chr5_2:27947966-27947985 Msa0766310:intron 40.0%
ACCAAAACCATTGCGATCAT+AGG + chr5_2:27947970-27947989 None:intergenic 40.0%
AGTCCATAACATTTGATGCC+TGG + chr5_2:27948193-27948212 None:intergenic 40.0%
!!! TTTCCCTTGTTTTCTTGGCT+AGG - chr5_2:27948510-27948529 Msa0766310:intron 40.0%
GTACCTAGCCAAGAAAACAA+GGG + chr5_2:27948516-27948535 None:intergenic 40.0%
AGTACCTAGCCAAGAAAACA+AGG + chr5_2:27948517-27948536 None:intergenic 40.0%
GTGAATGTAGCTTCATCAGT+TGG - chr5_2:27948637-27948656 Msa0766310:intron 40.0%
ATTGCATGAAGAAATGCCCA+GGG - chr5_2:27948771-27948790 Msa0766310:intron 40.0%
CATCTTCAATTAGAAGACCC+TGG + chr5_2:27948791-27948810 None:intergenic 40.0%
!! AAAAACCAGATGTTGACTCG+CGG - chr5_2:27948811-27948830 Msa0766310:CDS 40.0%
GACAGACCTCTTTACTTGAA+AGG - chr5_2:27948923-27948942 Msa0766310:five_prime_UTR 40.0%
GACCTCTTTACTTGAAAGGT+TGG - chr5_2:27948927-27948946 Msa0766310:five_prime_UTR 40.0%
! TCCCAACCTTTCAAGTAAAG+AGG + chr5_2:27948932-27948951 None:intergenic 40.0%
! GGGAGAGTGTGATATGATTA+TGG - chr5_2:27948948-27948967 Msa0766310:five_prime_UTR 40.0%
!! AGTGTGTTGGTTCTGGAGAT+TGG + chr5_2:27946367-27946386 None:intergenic 45.0%
AATCTCCAGAACCAACACAC+TGG - chr5_2:27946366-27946385 Msa0766310:three_prime_UTR 45.0%
!! GCGTGTTTGGTGTTCGATAT+GGG + chr5_2:27947031-27947050 None:intergenic 45.0%
!! TGCGTGTTTGGTGTTCGATA+TGG + chr5_2:27947032-27947051 None:intergenic 45.0%
GTGTGTGTTTCGTTAGGCAA+TGG - chr5_2:27947169-27947188 Msa0766310:intron 45.0%
ATGGTACTGCAACACATCCA+AGG - chr5_2:27947631-27947650 Msa0766310:CDS 45.0%
CTACTTACTGCAGTGCTACA+GGG + chr5_2:27947768-27947787 None:intergenic 45.0%
ACTACTTACTGCAGTGCTAC+AGG + chr5_2:27947769-27947788 None:intergenic 45.0%
!!! ATGGTTTTGGTCGTGGAGAA+AGG - chr5_2:27947979-27947998 Msa0766310:intron 45.0%
TTCTTGGCTAGGTACTTCCT+AGG - chr5_2:27948521-27948540 Msa0766310:intron 45.0%
CTCTCGAGTCTAAACTCCAA+AGG - chr5_2:27948552-27948571 Msa0766310:intron 45.0%
GATAAAACTCCCAAAGCCTG+AGG - chr5_2:27948741-27948760 Msa0766310:intron 45.0%
ATCACTTCACCTCAGGCTTT+GGG + chr5_2:27948753-27948772 None:intergenic 45.0%
AATCACTTCACCTCAGGCTT+TGG + chr5_2:27948754-27948773 None:intergenic 45.0%
TCATGCAATCACTTCACCTC+AGG + chr5_2:27948760-27948779 None:intergenic 45.0%
GATTGCATGAAGAAATGCCC+AGG - chr5_2:27948770-27948789 Msa0766310:intron 45.0%
!! AAAACCAGATGTTGACTCGC+GGG - chr5_2:27948812-27948831 Msa0766310:CDS 45.0%
TGCAAAACTAGCACCATCCA+TGG - chr5_2:27948839-27948858 Msa0766310:CDS 45.0%
!! AAAAAAAAAAAAAACAAATT+GGG + chr5_2:27946546-27946565 None:intergenic 5.0%
!! AAAAAAAAAAAAAAACAAAT+TGG + chr5_2:27946547-27946566 None:intergenic 5.0%
!!! ATGTATTTTTATATTTAAAA+TGG + chr5_2:27947456-27947475 None:intergenic 5.0%
CTCAGACACAGACACAGACA+CGG - chr5_2:27946644-27946663 Msa0766310:three_prime_UTR 50.0%
AATAAGGCGTGTCTCAGGGT+CGG + chr5_2:27946745-27946764 None:intergenic 50.0%
CTCGAATAAGGCGTGTCTCA+GGG + chr5_2:27946749-27946768 None:intergenic 50.0%
ACCGTTAGTGGCTAAAGCCA+AGG - chr5_2:27946874-27946893 Msa0766310:intron 50.0%
!! GCCTTGGCTTTAGCCACTAA+CGG + chr5_2:27946878-27946897 None:intergenic 50.0%
TAGGAAGCTGCTCAACGTGT+AGG - chr5_2:27947894-27947913 Msa0766310:intron 50.0%
AGGAAGCTGCTCAACGTGTA+GGG - chr5_2:27947895-27947914 Msa0766310:intron 50.0%
!!! GATCGCAATGGTTTTGGTCG+TGG - chr5_2:27947972-27947991 Msa0766310:intron 50.0%
AGAACGCCAAGACCTTCAAG+GGG + chr5_2:27949020-27949039 None:intergenic 50.0%
GAGAACGCCAAGACCTTCAA+GGG + chr5_2:27949021-27949040 None:intergenic 50.0%
AGAGAACGCCAAGACCTTCA+AGG + chr5_2:27949022-27949041 None:intergenic 50.0%
TGAAGGTCTTGGCGTTCTCT+TGG - chr5_2:27949022-27949041 Msa0766310:intron 50.0%
TCGGGAAACGCAAGCGTAGT+TGG - chr5_2:27946281-27946300 Msa0766310:three_prime_UTR 55.0%
AACGCAAGCGTAGTTGGTCG+TGG - chr5_2:27946287-27946306 Msa0766310:three_prime_UTR 55.0%
ACGCAAGCGTAGTTGGTCGT+GGG - chr5_2:27946288-27946307 Msa0766310:three_prime_UTR 55.0%
!! CGCAACCAGTGTGTTGGTTC+TGG + chr5_2:27946374-27946393 None:intergenic 55.0%
CCTCGAATAAGGCGTGTCTC+AGG + chr5_2:27946750-27946769 None:intergenic 55.0%
! CCTGAGACACGCCTTATTCG+AGG - chr5_2:27946747-27946766 Msa0766310:three_prime_UTR 55.0%
CTTACTGCAGTGCTACAGGG+AGG + chr5_2:27947765-27947784 None:intergenic 55.0%
GGAAGCTGCTCAACGTGTAG+GGG - chr5_2:27947896-27947915 Msa0766310:intron 55.0%
ACTCGAGAGTTCTCGCACCT+AGG + chr5_2:27948541-27948560 None:intergenic 55.0%
GAACGCCAAGACCTTCAAGG+GGG + chr5_2:27949019-27949038 None:intergenic 55.0%
TCCAAGACCGTATTCCGACG+CGG - chr5_2:27949052-27949071 Msa0766310:five_prime_UTR 55.0%
AGCTGCTCAACGTGTAGGGG+AGG - chr5_2:27947899-27947918 Msa0766310:intron 60.0%
TCAGCCCGCGAGTCAACATC+TGG + chr5_2:27948819-27948838 None:intergenic 60.0%
GCGTCGGAATACGGTCTTGG+AGG + chr5_2:27949053-27949072 None:intergenic 60.0%
CGTAGTTGGTCGTGGGTCGC+CGG - chr5_2:27946295-27946314 Msa0766310:three_prime_UTR 65.0%
GAAGAGTCCGGCCCCCTTGA+AGG - chr5_2:27949005-27949024 Msa0766310:five_prime_UTR 65.0%
TCCGGCCCCCTTGAAGGTCT+TGG - chr5_2:27949011-27949030 Msa0766310:five_prime_UTR 65.0%
GCCAAGACCTTCAAGGGGGC+CGG + chr5_2:27949015-27949034 None:intergenic 65.0%
CCCGCGTCGGAATACGGTCT+TGG + chr5_2:27949056-27949075 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 27946239 27949075 27946239 ID=Msa0766310;Name=Msa0766310
chr5_2 mRNA 27946239 27949075 27946239 ID=Msa0766310-mRNA-1;Parent=Msa0766310;Name=Msa0766310-mRNA-1;_AED=0.12;_eAED=0.12;_QI=153|1|1|1|0.6|0.5|6|544|166
chr5_2 exon 27946239 27946782 27946239 ID=Msa0766310-mRNA-1:exon:10880;Parent=Msa0766310-mRNA-1
chr5_2 exon 27947262 27947417 27947262 ID=Msa0766310-mRNA-1:exon:10879;Parent=Msa0766310-mRNA-1
chr5_2 exon 27947535 27947687 27947535 ID=Msa0766310-mRNA-1:exon:10878;Parent=Msa0766310-mRNA-1
chr5_2 exon 27948365 27948453 27948365 ID=Msa0766310-mRNA-1:exon:10877;Parent=Msa0766310-mRNA-1
chr5_2 exon 27948800 27949030 27948800 ID=Msa0766310-mRNA-1:exon:10876;Parent=Msa0766310-mRNA-1
chr5_2 exon 27949051 27949075 27949051 ID=Msa0766310-mRNA-1:exon:10875;Parent=Msa0766310-mRNA-1
chr5_2 five_prime_UTR 27949051 27949075 27949051 ID=Msa0766310-mRNA-1:five_prime_utr;Parent=Msa0766310-mRNA-1
chr5_2 five_prime_UTR 27948903 27949030 27948903 ID=Msa0766310-mRNA-1:five_prime_utr;Parent=Msa0766310-mRNA-1
chr5_2 CDS 27948800 27948902 27948800 ID=Msa0766310-mRNA-1:cds;Parent=Msa0766310-mRNA-1
chr5_2 CDS 27948365 27948453 27948365 ID=Msa0766310-mRNA-1:cds;Parent=Msa0766310-mRNA-1
chr5_2 CDS 27947535 27947687 27947535 ID=Msa0766310-mRNA-1:cds;Parent=Msa0766310-mRNA-1
chr5_2 CDS 27947262 27947417 27947262 ID=Msa0766310-mRNA-1:cds;Parent=Msa0766310-mRNA-1
chr5_2 three_prime_UTR 27946239 27946782 27946239 ID=Msa0766310-mRNA-1:three_prime_utr;Parent=Msa0766310-mRNA-1
Gene Sequence

>Msa0766310

ATGGTTCTAATGGTGAATTCATTTGAACTATTTCAATTCAATACTTCTTCTTTCTTCGGAGTTATCCCAAAACCATTCATTTCCTTTCAACCACAAAGTATAAGTTTAGTTAAGAAACCGTTAGTGGCTAAAGCCAAGGCAAATGCAAGAAAAGAAAGTGCTAAAATTCGAAACAGAAAGATGCAGAAAAAGTTTAATGGTACTGCAACACATCCAAGGCTTTCAGTATTTTGCTCAGATAAGCAATTGTATGCAATGCTTGTAGATGACCAAAATAAGAAGTGCTTATTCTATGGAAGCACGCTGCAGAAATCGTTTCGAGAAAATCCTCCCTGTAGCACTGCAGAAGCTGCTCAACGTGTAGGGGAGGCACTTGTGAAAGCCTGTGTTGATCTAAACATAAATGAAATATCATCCTATGATCGCAATGGTTTTGGTCGTGGAGAAAGGTTAAACGCCTTTGAGATTGCTATTTCCAATTATGGATTCTTGCCTAGATAG
Protein sequence

>Msa0766310

MVLMVNSFELFQFNTSSFFGVIPKPFISFQPQSISLVKKPLVAKAKANARKESAKIRNRKMQKKFNGTATHPRLSVFCSDKQLYAMLVDDQNKKCLFYGSTLQKSFRENPPCSTAEAAQRVGEALVKACVDLNINEISSYDRNGFGRGERLNAFEIAISNYGFLPR*