AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0921160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0921160 MtrunA17_Chr6g0477741 80.645 217 39 1 4 217 1 217 1.04e-125 354
Msa0921160 MtrunA17_Chr6g0477751 79.167 216 43 1 4 217 1 216 2.83e-125 353
Msa0921160 MtrunA17_Chr6g0452491 78.505 214 42 1 4 217 1 210 4.05e-122 344
Msa0921160 MtrunA17_Chr6g0452521 77.561 205 45 1 2 205 31 235 1.38e-116 332
Msa0921160 MtrunA17_Chr6g0476831 56.872 211 85 3 4 210 1 209 8.17e-68 208
Msa0921160 MtrunA17_Chr6g0476871 57.009 214 86 3 4 211 1 214 1.10e-60 189
Msa0921160 MtrunA17_Chr1g0163381 44.444 216 108 7 4 209 1 214 3.54e-49 160
Msa0921160 MtrunA17_Chr6g0476791 44.907 216 107 6 1 207 1 213 6.67e-47 154
Msa0921160 MtrunA17_Chr6g0476921 41.848 184 94 6 30 206 26 203 2.00e-32 116
Msa0921160 MtrunA17_Chr6g0476951 43.750 208 98 9 4 205 1 195 2.27e-32 116
Msa0921160 MtrunA17_Chr6g0476961 42.439 205 100 8 8 205 1 194 2.07e-30 111
Msa0921160 MtrunA17_Chr6g0477281 41.546 207 89 6 4 205 1 180 4.77e-29 107
Msa0921160 MtrunA17_Chr6g0457121 56.250 96 40 2 4 98 26 120 1.26e-28 104
Msa0921160 MtrunA17_Chr3g0104591 38.421 190 100 7 32 212 29 210 1.47e-22 91.3
Msa0921160 MtrunA17_Chr6g0467611 36.444 225 118 12 4 212 1 216 3.53e-22 90.5
Msa0921160 MtrunA17_Chr6g0467621 37.391 230 109 13 4 212 1 216 6.46e-22 89.7
Msa0921160 MtrunA17_Chr6g0467761 36.889 225 117 12 4 212 1 216 6.75e-22 89.7
Msa0921160 MtrunA17_Chr6g0467771 36.239 218 115 9 4 208 1 207 9.75e-22 89.4
Msa0921160 MtrunA17_Chr6g0467781 36.686 169 81 9 55 209 13 169 1.79e-14 68.6
Msa0921160 MtrunA17_Chr2g0314431 33.175 211 109 12 4 203 5 194 4.94e-13 65.5
Msa0921160 MtrunA17_Chr6g0481911 31.863 204 125 9 10 205 6 203 9.56e-13 64.7
Msa0921160 MtrunA17_Chr6g0476821 40.000 130 68 6 91 215 21 145 3.52e-12 62.0
Msa0921160 MtrunA17_Chr2g0314441 32.394 213 116 11 3 205 4 198 7.31e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTAGGGTTATCACTTTC+TGG 0.161357 6_2:-58018198 None:intergenic
CTGAATCACCAACTTTGTTT+AGG 0.218681 6_2:-58018233 None:intergenic
ATGGATCTGGTAATGTTTAT+AGG 0.227385 6_2:+58018514 Msa0921160:CDS
ATACAAGATATTGCTGTAAT+AGG 0.268557 6_2:+58018273 Msa0921160:CDS
CCTCTGATTGTCTCTATTCC+AGG 0.274561 6_2:-58018471 None:intergenic
ACTGGAGATTGTGCTTATAT+TGG 0.278706 6_2:+58018558 Msa0921160:CDS
TGAATCACCAACTTTGTTTA+GGG 0.302425 6_2:-58018232 None:intergenic
TCTAGTATGGTGAATTTAAC+TGG 0.302951 6_2:-58018300 None:intergenic
AGCATTGTTTGGTGAAATAT+TGG 0.343828 6_2:-58018112 None:intergenic
GAGGGATAAAATAGGTTTCA+TGG 0.353544 6_2:-58018058 None:intergenic
GAGCAAGTATTGGACATAAA+TGG 0.360089 6_2:+58018138 Msa0921160:CDS
GGACTGCAACTGTTTAGTTC+TGG 0.363850 6_2:+58018579 Msa0921160:CDS
TACTAGAACTCATACTAAAT+AGG 0.365223 6_2:-58018696 None:intergenic
TGGATCTGGTAATGTTTATA+GGG 0.365536 6_2:+58018515 Msa0921160:CDS
GATGCTGCATCTATTAAATC+TGG 0.371349 6_2:+58018660 Msa0921160:CDS
ATATCTTGTATGACAGTTAC+TGG 0.377987 6_2:-58018261 None:intergenic
TCTATTCCAGGATAGTTTGC+AGG 0.388966 6_2:-58018459 None:intergenic
TATTGGTGATAAAAGCAAAT+AGG 0.390605 6_2:-58018095 None:intergenic
TTTGATGATTCAGCACAATC+TGG 0.398835 6_2:-58018381 None:intergenic
ACTTGCTCAACAGCATTGTT+TGG 0.401563 6_2:-58018123 None:intergenic
TCTGGTAATGTTTATAGGGT+TGG 0.416063 6_2:+58018519 Msa0921160:CDS
GAAAGGGTGAGGGATAAAAT+AGG 0.419184 6_2:-58018066 None:intergenic
CAACTAGCTTGTGTTGGTAT+TGG 0.427835 6_2:+58018432 Msa0921160:CDS
CTAGCTTGTGTTGGTATTGG+TGG 0.441922 6_2:+58018435 Msa0921160:CDS
TATTGGCCACCAGGGGTAAG+GGG 0.451454 6_2:-58018165 None:intergenic
GGTGGTCCTGCAAACTATCC+TGG 0.456364 6_2:+58018453 Msa0921160:CDS
TTAATAGTGAAGCATGGATC+TGG 0.460803 6_2:+58018501 Msa0921160:CDS
AGTATTGGCCACCAGGGGTA+AGG 0.463701 6_2:-58018167 None:intergenic
AGGGTTAGTCCTCCAATACT+AGG 0.474991 6_2:-58018213 None:intergenic
ATTGGCCACCAGGGGTAAGG+GGG 0.476496 6_2:-58018164 None:intergenic
AGCAAATAGGAAGAAGGAAA+GGG 0.481208 6_2:-58018082 None:intergenic
CAATGCTGTTGAGCAAGTAT+TGG 0.481709 6_2:+58018128 Msa0921160:CDS
TTTCTGGTAAAATGTAGTAT+TGG 0.484403 6_2:-58018182 None:intergenic
AAGCAAATAGGAAGAAGGAA+AGG 0.491844 6_2:-58018083 None:intergenic
GAAAGTGATAACCCTAGTAT+TGG 0.495230 6_2:+58018201 Msa0921160:CDS
AAAATGTAGTATTGGCCACC+AGG 0.496116 6_2:-58018174 None:intergenic
ATTGTGCTGAATCATCAAAA+TGG 0.497723 6_2:+58018385 Msa0921160:CDS
GTATTGGCCACCAGGGGTAA+GGG 0.502071 6_2:-58018166 None:intergenic
AGTGATAACCCTAGTATTGG+AGG 0.519798 6_2:+58018204 Msa0921160:CDS
GATAAAAGCAAATAGGAAGA+AGG 0.526672 6_2:-58018088 None:intergenic
CTGTTTAGTTCTGGTGAAGG+AGG 0.540270 6_2:+58018588 Msa0921160:CDS
ATAGGAAGAAGGAAAGGGTG+AGG 0.540395 6_2:-58018077 None:intergenic
GGACTAACCCTAAACAAAGT+TGG 0.540688 6_2:+58018225 Msa0921160:CDS
GGGTTAGTCCTCCAATACTA+GGG 0.545234 6_2:-58018212 None:intergenic
ATCACTAGTAACATCGTGAC+AGG 0.548264 6_2:+58018327 Msa0921160:CDS
ATACAACAGAATAAGCATCA+AGG 0.559870 6_2:-58018632 None:intergenic
TAGGAAGAAGGAAAGGGTGA+GGG 0.562566 6_2:-58018076 None:intergenic
TACAACAGAATAAGCATCAA+GGG 0.562914 6_2:-58018631 None:intergenic
TTGGCCACCAGGGGTAAGGG+GGG 0.568615 6_2:-58018163 None:intergenic
AGTGAACTCGATCTCAAGAT+CGG 0.599342 6_2:-58018352 None:intergenic
AATACTCAACTAGCTTGTGT+TGG 0.603192 6_2:+58018426 Msa0921160:CDS
CAACTGTTTAGTTCTGGTGA+AGG 0.626732 6_2:+58018585 Msa0921160:CDS
AAATGTAGTATTGGCCACCA+GGG 0.645099 6_2:-58018173 None:intergenic
CCTGGAATAGAGACAATCAG+AGG 0.673236 6_2:+58018471 Msa0921160:CDS
AATGTAGTATTGGCCACCAG+GGG 0.700291 6_2:-58018172 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGGTGATAAAAGCAAAT+AGG - chr6_2:58018098-58018117 None:intergenic 25.0%
!! TTTCTGGTAAAATGTAGTAT+TGG - chr6_2:58018185-58018204 None:intergenic 25.0%
! ATACAAGATATTGCTGTAAT+AGG + chr6_2:58018273-58018292 Msa0921160:CDS 25.0%
!! AAGTTTTTAATAGTGAAGCA+TGG + chr6_2:58018495-58018514 Msa0921160:CDS 25.0%
GATAAAAGCAAATAGGAAGA+AGG - chr6_2:58018091-58018110 None:intergenic 30.0%
! AGCATTGTTTGGTGAAATAT+TGG - chr6_2:58018115-58018134 None:intergenic 30.0%
! TGAATCACCAACTTTGTTTA+GGG - chr6_2:58018235-58018254 None:intergenic 30.0%
ATATCTTGTATGACAGTTAC+TGG - chr6_2:58018264-58018283 None:intergenic 30.0%
TCTAGTATGGTGAATTTAAC+TGG - chr6_2:58018303-58018322 None:intergenic 30.0%
!! ACTAGTGATGTTTTCTAGTA+TGG - chr6_2:58018316-58018335 None:intergenic 30.0%
ATTGTGCTGAATCATCAAAA+TGG + chr6_2:58018385-58018404 Msa0921160:CDS 30.0%
! ATGGATCTGGTAATGTTTAT+AGG + chr6_2:58018514-58018533 Msa0921160:CDS 30.0%
! TGGATCTGGTAATGTTTATA+GGG + chr6_2:58018515-58018534 Msa0921160:CDS 30.0%
! TTATAGGGTTGGATTTTGTT+TGG + chr6_2:58018530-58018549 Msa0921160:CDS 30.0%
TACAACAGAATAAGCATCAA+GGG - chr6_2:58018634-58018653 None:intergenic 30.0%
ATACAACAGAATAAGCATCA+AGG - chr6_2:58018635-58018654 None:intergenic 30.0%
GAGGGATAAAATAGGTTTCA+TGG - chr6_2:58018061-58018080 None:intergenic 35.0%
AGCAAATAGGAAGAAGGAAA+GGG - chr6_2:58018085-58018104 None:intergenic 35.0%
AAGCAAATAGGAAGAAGGAA+AGG - chr6_2:58018086-58018105 None:intergenic 35.0%
!! GAGCAAGTATTGGACATAAA+TGG + chr6_2:58018138-58018157 Msa0921160:CDS 35.0%
ATACTAGGGTTATCACTTTC+TGG - chr6_2:58018201-58018220 None:intergenic 35.0%
GAAAGTGATAACCCTAGTAT+TGG + chr6_2:58018201-58018220 Msa0921160:CDS 35.0%
! CTGAATCACCAACTTTGTTT+AGG - chr6_2:58018236-58018255 None:intergenic 35.0%
! TTTGATGATTCAGCACAATC+TGG - chr6_2:58018384-58018403 None:intergenic 35.0%
! AATACTCAACTAGCTTGTGT+TGG + chr6_2:58018426-58018445 Msa0921160:CDS 35.0%
TTAATAGTGAAGCATGGATC+TGG + chr6_2:58018501-58018520 Msa0921160:CDS 35.0%
! TCTGGTAATGTTTATAGGGT+TGG + chr6_2:58018519-58018538 Msa0921160:CDS 35.0%
!! GGATTTTGTTTGGATACAAC+TGG + chr6_2:58018540-58018559 Msa0921160:CDS 35.0%
ACTGGAGATTGTGCTTATAT+TGG + chr6_2:58018558-58018577 Msa0921160:CDS 35.0%
GATGCTGCATCTATTAAATC+TGG + chr6_2:58018660-58018679 Msa0921160:CDS 35.0%
GAAAGGGTGAGGGATAAAAT+AGG - chr6_2:58018069-58018088 None:intergenic 40.0%
ACTTGCTCAACAGCATTGTT+TGG - chr6_2:58018126-58018145 None:intergenic 40.0%
CAATGCTGTTGAGCAAGTAT+TGG + chr6_2:58018128-58018147 Msa0921160:CDS 40.0%
!! AAATGTAGTATTGGCCACCA+GGG - chr6_2:58018176-58018195 None:intergenic 40.0%
!! AAAATGTAGTATTGGCCACC+AGG - chr6_2:58018177-58018196 None:intergenic 40.0%
! AGTGATAACCCTAGTATTGG+AGG + chr6_2:58018204-58018223 Msa0921160:CDS 40.0%
GGACTAACCCTAAACAAAGT+TGG + chr6_2:58018225-58018244 Msa0921160:CDS 40.0%
ATCACTAGTAACATCGTGAC+AGG + chr6_2:58018327-58018346 Msa0921160:CDS 40.0%
AGTGAACTCGATCTCAAGAT+CGG - chr6_2:58018355-58018374 None:intergenic 40.0%
! CAACTAGCTTGTGTTGGTAT+TGG + chr6_2:58018432-58018451 Msa0921160:CDS 40.0%
! TCTATTCCAGGATAGTTTGC+AGG - chr6_2:58018462-58018481 None:intergenic 40.0%
!! CAACTGTTTAGTTCTGGTGA+AGG + chr6_2:58018585-58018604 Msa0921160:CDS 40.0%
TAGGAAGAAGGAAAGGGTGA+GGG - chr6_2:58018079-58018098 None:intergenic 45.0%
ATAGGAAGAAGGAAAGGGTG+AGG - chr6_2:58018080-58018099 None:intergenic 45.0%
!! AATGTAGTATTGGCCACCAG+GGG - chr6_2:58018175-58018194 None:intergenic 45.0%
!! GGGTTAGTCCTCCAATACTA+GGG - chr6_2:58018215-58018234 None:intergenic 45.0%
!! AGGGTTAGTCCTCCAATACT+AGG - chr6_2:58018216-58018235 None:intergenic 45.0%
! CTAGCTTGTGTTGGTATTGG+TGG + chr6_2:58018435-58018454 Msa0921160:CDS 45.0%
CCTCTGATTGTCTCTATTCC+AGG - chr6_2:58018474-58018493 None:intergenic 45.0%
CCTGGAATAGAGACAATCAG+AGG + chr6_2:58018471-58018490 Msa0921160:CDS 45.0%
! GGACTGCAACTGTTTAGTTC+TGG + chr6_2:58018579-58018598 Msa0921160:CDS 45.0%
!! CTGTTTAGTTCTGGTGAAGG+AGG + chr6_2:58018588-58018607 Msa0921160:CDS 45.0%
TATTGGCCACCAGGGGTAAG+GGG - chr6_2:58018168-58018187 None:intergenic 55.0%
! GTATTGGCCACCAGGGGTAA+GGG - chr6_2:58018169-58018188 None:intergenic 55.0%
!! AGTATTGGCCACCAGGGGTA+AGG - chr6_2:58018170-58018189 None:intergenic 55.0%
GGTGGTCCTGCAAACTATCC+TGG + chr6_2:58018453-58018472 Msa0921160:CDS 55.0%
AATGGTACCCCCCTTACCCC+TGG + chr6_2:58018156-58018175 Msa0921160:CDS 60.0%
ATTGGCCACCAGGGGTAAGG+GGG - chr6_2:58018167-58018186 None:intergenic 60.0%
TTGGCCACCAGGGGTAAGGG+GGG - chr6_2:58018166-58018185 None:intergenic 65.0%
GGTACCCCCCTTACCCCTGG+TGG + chr6_2:58018159-58018178 Msa0921160:CDS 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 58018051 58018704 58018051 ID=Msa0921160;Name=Msa0921160
chr6_2 mRNA 58018051 58018704 58018051 ID=Msa0921160-mRNA-1;Parent=Msa0921160;Name=Msa0921160-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|-1|0|1|-1|1|1|0|217
chr6_2 exon 58018051 58018704 58018051 ID=Msa0921160-mRNA-1:exon:8640;Parent=Msa0921160-mRNA-1
chr6_2 CDS 58018051 58018704 58018051 ID=Msa0921160-mRNA-1:cds;Parent=Msa0921160-mRNA-1
Gene Sequence

>Msa0921160

ATGGAAACCATGAAACCTATTTTATCCCTCACCCTTTCCTTCTTCCTATTTGCTTTTATCACCAATATTTCACCAAACAATGCTGTTGAGCAAGTATTGGACATAAATGGTACCCCCCTTACCCCTGGTGGCCAATACTACATTTTACCAGAAAGTGATAACCCTAGTATTGGAGGACTAACCCTAAACAAAGTTGGTGATTCAGAATGTCCAGTAACTGTCATACAAGATATTGCTGTAATAGGTTTACCAGTTAAATTCACCATACTAGAAAACATCACTAGTAACATCGTGACAGGTACCGATCTTGAGATCGAGTTCACTAAGAAGCCAGATTGTGCTGAATCATCAAAATGGTTGATGTTTGTTGATCATAATACTCAACTAGCTTGTGTTGGTATTGGTGGTCCTGCAAACTATCCTGGAATAGAGACAATCAGAGGAAAGTTTTTAATAGTGAAGCATGGATCTGGTAATGTTTATAGGGTTGGATTTTGTTTGGATACAACTGGAGATTGTGCTTATATTGGACTGCAACTGTTTAGTTCTGGTGAAGGAGGATCACGTTTAATTTTGACTGCCCTTGATGCTTATTCTGTTGTATTTGTTGATGCTGCATCTATTAAATCTGGATCACGTGCATTGCCTATTTAG

Protein sequence

>Msa0921160

METMKPILSLTLSFFLFAFITNISPNNAVEQVLDINGTPLTPGGQYYILPESDNPSIGGLTLNKVGDSECPVTVIQDIAVIGLPVKFTILENITSNIVTGTDLEIEFTKKPDCAESSKWLMFVDHNTQLACVGIGGPANYPGIETIRGKFLIVKHGSGNVYRVGFCLDTTGDCAYIGLQLFSSGEGGSRLILTALDAYSVVFVDAASIKSGSRALPI*