AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1018960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1018960 MtrunA17_Chr7g0234441 97.576 165 4 0 1 165 1 165 1.28e-112 317
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1018960 AT5G48480.1 53.676 136 53 4 21 152 23 152 1.11e-34 119

Find 57 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTTCTTCTTGGTTCTTT+TGG 0.216551 7_1:-39960305 Msa1018960:three_prime_UTR
GGAGGAAAATTATTATCTTA+TGG 0.272821 7_1:-39960195 Msa1018960:three_prime_UTR
GTTATTATCCCTATGAGTTT+AGG 0.284592 7_1:-39960270 Msa1018960:three_prime_UTR
AGAAAAGACAAGTTTCTTCT+TGG 0.303382 7_1:-39960314 Msa1018960:three_prime_UTR
TCACCAACAGCTTTCTTTGC+AGG 0.304196 7_1:+39960453 None:intergenic
AACAACGGGAGAAGCGGTTT+CGG 0.346996 7_1:+39961435 None:intergenic
TTGAAGGAAGGAAGAGAGAT+TGG 0.349549 7_1:+39961494 None:intergenic
CGCGTGTGGTGGCGGGCGCG+TGG 0.374312 7_1:-39960520 Msa1018960:CDS
CTTGATCAGCCTTACGCTTA+GGG 0.374905 7_1:+39961302 None:intergenic
TCTTGATCAGCCTTACGCTT+AGG 0.390293 7_1:+39961301 None:intergenic
GGTGGTACTTTACTGCGTTA+TGG 0.400467 7_1:-39960216 Msa1018960:three_prime_UTR
GACAGAATACCTAAACTCAT+AGG 0.409718 7_1:+39960261 None:intergenic
TGTGAAGGCGCGTGTGGTGG+CGG 0.419302 7_1:-39960528 Msa1018960:CDS
ACAACAGCGTCGTTAGCTTT+CGG 0.425159 7_1:+39961373 None:intergenic
TGGTGGCGGGCGCGTGGGAA+AGG 0.428433 7_1:-39960514 Msa1018960:CDS
CTGATCCATACGGTTACGTT+TGG 0.429548 7_1:-39960488 Msa1018960:CDS
GTGAAGGCGCGTGTGGTGGC+GGG 0.450780 7_1:-39960527 Msa1018960:CDS
AATGAAATGAGAAGAGTATG+AGG 0.457339 7_1:+39961552 None:intergenic
AAATTTAAATTCAACTTCTA+CGG 0.472728 7_1:+39960387 None:intergenic
TGAGTTTAGGTATTCTGTCT+AGG 0.473176 7_1:-39960257 Msa1018960:three_prime_UTR
GCGTGTGGTGGCGGGCGCGT+GGG 0.474181 7_1:-39960519 Msa1018960:CDS
ATAGCAAAGGCAGTGAGTGC+TGG 0.474235 7_1:-39960579 Msa1018960:CDS
GGGAGAAGCGGTTTCGGAAA+CGG 0.476505 7_1:+39961441 None:intergenic
TCTCTTCCTTCCTTCAACAA+TGG 0.480974 7_1:-39961488 Msa1018960:exon
GTTTCGGAAGCCATTGTTGA+AGG 0.481782 7_1:+39961478 None:intergenic
AAAGCGACGAAGGAAACAAC+GGG 0.492629 7_1:+39961421 None:intergenic
CGGAAGCCATTGTTGAAGGA+AGG 0.501044 7_1:+39961482 None:intergenic
ACAGAATACCTAAACTCATA+GGG 0.559871 7_1:+39960262 None:intergenic
AGTTCAGCAGAGAGAACAAG+AGG 0.563515 7_1:+39961274 None:intergenic
TCTGCTGAACTCAAGATCGC+TGG 0.565708 7_1:-39961262 Msa1018960:CDS
TGGAACAGTGTGAAGAGTGG+TGG 0.570663 7_1:-39960648 Msa1018960:intron
GCTTCCGAAACTCCTCAAAA+CGG 0.583220 7_1:-39961466 Msa1018960:CDS
GAGTGTGAAGGCGCGTGTGG+TGG 0.585208 7_1:-39960531 Msa1018960:CDS
AATTGCCAAACGTAACCGTA+TGG 0.585974 7_1:+39960483 None:intergenic
ATGAAATGAGAAGAGTATGA+GGG 0.586793 7_1:+39961553 None:intergenic
CTAGGAGAGAGAAAATGTGA+GGG 0.591334 7_1:+39961522 None:intergenic
AGTGTGAAGAGTGGTGGAAA+CGG 0.593792 7_1:-39960642 Msa1018960:CDS
GGAGCTTCAACGAAGAGCTG+AGG 0.596211 7_1:+39961394 None:intergenic
CATCAACAGCATCAGCAACA+AGG 0.596823 7_1:+39961230 None:intergenic
TCAGATCATCTTCACATCAT+CGG 0.598872 7_1:+39960410 None:intergenic
CTGAGGCTTGAAAGCGACGA+AGG 0.599489 7_1:+39961411 None:intergenic
AACAGCATCAGCAACAAGGA+AGG 0.600915 7_1:+39961234 None:intergenic
CATTGAAGCTGCGATAGCAA+AGG 0.613057 7_1:-39960592 Msa1018960:CDS
AAAAGAAACTCACGGTTTAG+CGG 0.615319 7_1:+39961204 None:intergenic
GAAGAGAGATTGGAAACCCT+AGG 0.616429 7_1:+39961504 None:intergenic
GAAGGAAACAACGGGAGAAG+CGG 0.617920 7_1:+39961429 None:intergenic
GGTACTTTACTGCGTTATGG+AGG 0.625827 7_1:-39960213 Msa1018960:three_prime_UTR
ACTCCTGCAAAGAAAGCTGT+TGG 0.629499 7_1:-39960456 Msa1018960:CDS
GCTGAGTGTGAAGGCGCGTG+TGG 0.631571 7_1:-39960534 Msa1018960:CDS
GGAAAGGTGACTGATCCATA+CGG 0.651721 7_1:-39960498 Msa1018960:CDS
GAAAGCTGTTGGTGACGTGG+AGG 0.652063 7_1:-39960445 Msa1018960:CDS
CCTAGGAGAGAGAAAATGTG+AGG 0.663056 7_1:+39961521 None:intergenic
GAAAGCGACGAAGGAAACAA+CGG 0.664481 7_1:+39961420 None:intergenic
GGTGAAGTAGCTGAGTGTGA+AGG 0.666417 7_1:-39960543 Msa1018960:CDS
AAAGAAAGCTGTTGGTGACG+TGG 0.670793 7_1:-39960448 Msa1018960:CDS
AGTGCTGGAGCAGTTGCTGA+AGG 0.674380 7_1:-39960564 Msa1018960:CDS
TAGGAGAGAGAAAATGTGAG+GGG 0.687361 7_1:+39961523 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTATAATTATTTT+TGG - chr7_1:39961027-39961046 Msa1018960:intron 0.0%
!!! ATTTTTTTTATAATTATTTT+TGG - chr7_1:39961027-39961046 Msa1018960:intron 0.0%
!! AAAAACAAAATCAAAGATTA+CGG + chr7_1:39960858-39960877 None:intergenic 15.0%
!! AAAACAGTAAAATAAAAGTA+AGG + chr7_1:39960885-39960904 None:intergenic 15.0%
!! AAATTTAAATTCAACTTCTA+CGG + chr7_1:39961311-39961330 None:intergenic 15.0%
!! AAAAACAAAATCAAAGATTA+CGG + chr7_1:39960858-39960877 None:intergenic 15.0%
!! AAAACAGTAAAATAAAAGTA+AGG + chr7_1:39960885-39960904 None:intergenic 15.0%
!! AAATTTAAATTCAACTTCTA+CGG + chr7_1:39961311-39961330 None:intergenic 15.0%
!! CAAATGTAATGAAATAACTA+CGG + chr7_1:39960650-39960669 None:intergenic 20.0%
!! AAAAACTGTAGCATAATATA+AGG + chr7_1:39960811-39960830 None:intergenic 20.0%
!!! TCTAGGTTAATATTTTGATA+TGG - chr7_1:39961455-39961474 Msa1018960:CDS 20.0%
!! CAAATGTAATGAAATAACTA+CGG + chr7_1:39960650-39960669 None:intergenic 20.0%
!! AAAAACTGTAGCATAATATA+AGG + chr7_1:39960811-39960830 None:intergenic 20.0%
!!! TCTAGGTTAATATTTTGATA+TGG - chr7_1:39961455-39961474 Msa1018960:CDS 20.0%
! TTTCAGATCTACAACAAAAA+AGG + chr7_1:39960532-39960551 None:intergenic 25.0%
!!! TTTAGTTTTTTGTTTTGTGC+TGG - chr7_1:39960829-39960848 Msa1018960:intron 25.0%
!!! AGGTTAATATTTTGATATGG+TGG - chr7_1:39961458-39961477 Msa1018960:CDS 25.0%
!!! TTAATATTTTGATATGGTGG+TGG - chr7_1:39961461-39961480 Msa1018960:CDS 25.0%
! GGAGGAAAATTATTATCTTA+TGG - chr7_1:39961500-39961519 Msa1018960:five_prime_UTR 25.0%
!! GATGAATGAATGAATTTTCT+TGG - chr7_1:39961526-39961545 Msa1018960:five_prime_UTR 25.0%
! TTTCAGATCTACAACAAAAA+AGG + chr7_1:39960532-39960551 None:intergenic 25.0%
!!! TTTAGTTTTTTGTTTTGTGC+TGG - chr7_1:39960829-39960848 Msa1018960:intron 25.0%
!!! AGGTTAATATTTTGATATGG+TGG - chr7_1:39961458-39961477 Msa1018960:CDS 25.0%
!!! TTAATATTTTGATATGGTGG+TGG - chr7_1:39961461-39961480 Msa1018960:CDS 25.0%
! GGAGGAAAATTATTATCTTA+TGG - chr7_1:39961500-39961519 Msa1018960:five_prime_UTR 25.0%
!! GATGAATGAATGAATTTTCT+TGG - chr7_1:39961526-39961545 Msa1018960:five_prime_UTR 25.0%
GTTATGTCATGTTTATAGCT+AGG - chr7_1:39960693-39960712 Msa1018960:intron 30.0%
!!! GCTTGATCTGTTTTTAGATA+AGG - chr7_1:39960769-39960788 Msa1018960:intron 30.0%
! AAGAAGAAACTTGTCTTTTC+TGG + chr7_1:39961382-39961401 None:intergenic 30.0%
AGAAAAGACAAGTTTCTTCT+TGG - chr7_1:39961381-39961400 Msa1018960:CDS 30.0%
!! AAGTTTCTTCTTGGTTCTTT+TGG - chr7_1:39961390-39961409 Msa1018960:CDS 30.0%
GTTATTATCCCTATGAGTTT+AGG - chr7_1:39961425-39961444 Msa1018960:CDS 30.0%
ACAGAATACCTAAACTCATA+GGG + chr7_1:39961436-39961455 None:intergenic 30.0%
GTTATGTCATGTTTATAGCT+AGG - chr7_1:39960693-39960712 Msa1018960:intron 30.0%
!!! GCTTGATCTGTTTTTAGATA+AGG - chr7_1:39960769-39960788 Msa1018960:intron 30.0%
! AAGAAGAAACTTGTCTTTTC+TGG + chr7_1:39961382-39961401 None:intergenic 30.0%
AGAAAAGACAAGTTTCTTCT+TGG - chr7_1:39961381-39961400 Msa1018960:CDS 30.0%
!! AAGTTTCTTCTTGGTTCTTT+TGG - chr7_1:39961390-39961409 Msa1018960:CDS 30.0%
GTTATTATCCCTATGAGTTT+AGG - chr7_1:39961425-39961444 Msa1018960:CDS 30.0%
ACAGAATACCTAAACTCATA+GGG + chr7_1:39961436-39961455 None:intergenic 30.0%
AAAAGAAACTCACGGTTTAG+CGG + chr7_1:39960494-39960513 None:intergenic 35.0%
GTAGTGAGAAAAGAAACTCA+CGG + chr7_1:39960502-39960521 None:intergenic 35.0%
CAAAACGAAAATCACGACTA+TGG + chr7_1:39960614-39960633 None:intergenic 35.0%
TCAGATCATCTTCACATCAT+CGG + chr7_1:39961288-39961307 None:intergenic 35.0%
GACAGAATACCTAAACTCAT+AGG + chr7_1:39961437-39961456 None:intergenic 35.0%
TGAGTTTAGGTATTCTGTCT+AGG - chr7_1:39961438-39961457 Msa1018960:CDS 35.0%
AAAAGAAACTCACGGTTTAG+CGG + chr7_1:39960494-39960513 None:intergenic 35.0%
GTAGTGAGAAAAGAAACTCA+CGG + chr7_1:39960502-39960521 None:intergenic 35.0%
CAAAACGAAAATCACGACTA+TGG + chr7_1:39960614-39960633 None:intergenic 35.0%
TCAGATCATCTTCACATCAT+CGG + chr7_1:39961288-39961307 None:intergenic 35.0%
GACAGAATACCTAAACTCAT+AGG + chr7_1:39961437-39961456 None:intergenic 35.0%
TGAGTTTAGGTATTCTGTCT+AGG - chr7_1:39961438-39961457 Msa1018960:CDS 35.0%
TAGGAGAGAGAAAATGTGAG+GGG + chr7_1:39960175-39960194 None:intergenic 40.0%
CTAGGAGAGAGAAAATGTGA+GGG + chr7_1:39960176-39960195 None:intergenic 40.0%
! CTCACATTTTCTCTCTCCTA+GGG - chr7_1:39960175-39960194 Msa1018960:three_prime_UTR 40.0%
TTGAAGGAAGGAAGAGAGAT+TGG + chr7_1:39960204-39960223 None:intergenic 40.0%
TCTCTTCCTTCCTTCAACAA+TGG - chr7_1:39960207-39960226 Msa1018960:three_prime_UTR 40.0%
!!! TTTTGGAACAGTGTGAAGAG+TGG - chr7_1:39961044-39961063 Msa1018960:intron 40.0%
AATTGCCAAACGTAACCGTA+TGG + chr7_1:39961215-39961234 None:intergenic 40.0%
TAGGAGAGAGAAAATGTGAG+GGG + chr7_1:39960175-39960194 None:intergenic 40.0%
CTAGGAGAGAGAAAATGTGA+GGG + chr7_1:39960176-39960195 None:intergenic 40.0%
! CTCACATTTTCTCTCTCCTA+GGG - chr7_1:39960175-39960194 Msa1018960:three_prime_UTR 40.0%
TTGAAGGAAGGAAGAGAGAT+TGG + chr7_1:39960204-39960223 None:intergenic 40.0%
TCTCTTCCTTCCTTCAACAA+TGG - chr7_1:39960207-39960226 Msa1018960:three_prime_UTR 40.0%
!!! TTTTGGAACAGTGTGAAGAG+TGG - chr7_1:39961044-39961063 Msa1018960:intron 40.0%
AATTGCCAAACGTAACCGTA+TGG + chr7_1:39961215-39961234 None:intergenic 40.0%
CCTAGGAGAGAGAAAATGTG+AGG + chr7_1:39960177-39960196 None:intergenic 45.0%
! CCTCACATTTTCTCTCTCCT+AGG - chr7_1:39960174-39960193 Msa1018960:three_prime_UTR 45.0%
GAAGAGAGATTGGAAACCCT+AGG + chr7_1:39960194-39960213 None:intergenic 45.0%
GTTTCGGAAGCCATTGTTGA+AGG + chr7_1:39960220-39960239 None:intergenic 45.0%
GCTTCCGAAACTCCTCAAAA+CGG - chr7_1:39960229-39960248 Msa1018960:three_prime_UTR 45.0%
AAAGCGACGAAGGAAACAAC+GGG + chr7_1:39960277-39960296 None:intergenic 45.0%
GAAAGCGACGAAGGAAACAA+CGG + chr7_1:39960278-39960297 None:intergenic 45.0%
!! ACAACAGCGTCGTTAGCTTT+CGG + chr7_1:39960325-39960344 None:intergenic 45.0%
CTTGATCAGCCTTACGCTTA+GGG + chr7_1:39960396-39960415 None:intergenic 45.0%
TCTTGATCAGCCTTACGCTT+AGG + chr7_1:39960397-39960416 None:intergenic 45.0%
AGTTCAGCAGAGAGAACAAG+AGG + chr7_1:39960424-39960443 None:intergenic 45.0%
AACAGCATCAGCAACAAGGA+AGG + chr7_1:39960464-39960483 None:intergenic 45.0%
CATCAACAGCATCAGCAACA+AGG + chr7_1:39960468-39960487 None:intergenic 45.0%
! AGTGTGAAGAGTGGTGGAAA+CGG - chr7_1:39961053-39961072 Msa1018960:intron 45.0%
CATTGAAGCTGCGATAGCAA+AGG - chr7_1:39961103-39961122 Msa1018960:intron 45.0%
GGAAAGGTGACTGATCCATA+CGG - chr7_1:39961197-39961216 Msa1018960:intron 45.0%
CTGATCCATACGGTTACGTT+TGG - chr7_1:39961207-39961226 Msa1018960:intron 45.0%
ACTCCTGCAAAGAAAGCTGT+TGG - chr7_1:39961239-39961258 Msa1018960:CDS 45.0%
TCACCAACAGCTTTCTTTGC+AGG + chr7_1:39961245-39961264 None:intergenic 45.0%
!! AAAGAAAGCTGTTGGTGACG+TGG - chr7_1:39961247-39961266 Msa1018960:CDS 45.0%
GGTGGTACTTTACTGCGTTA+TGG - chr7_1:39961479-39961498 Msa1018960:exon 45.0%
GGTACTTTACTGCGTTATGG+AGG - chr7_1:39961482-39961501 Msa1018960:exon 45.0%
CCTAGGAGAGAGAAAATGTG+AGG + chr7_1:39960177-39960196 None:intergenic 45.0%
! CCTCACATTTTCTCTCTCCT+AGG - chr7_1:39960174-39960193 Msa1018960:three_prime_UTR 45.0%
GAAGAGAGATTGGAAACCCT+AGG + chr7_1:39960194-39960213 None:intergenic 45.0%
GTTTCGGAAGCCATTGTTGA+AGG + chr7_1:39960220-39960239 None:intergenic 45.0%
GCTTCCGAAACTCCTCAAAA+CGG - chr7_1:39960229-39960248 Msa1018960:three_prime_UTR 45.0%
AAAGCGACGAAGGAAACAAC+GGG + chr7_1:39960277-39960296 None:intergenic 45.0%
GAAAGCGACGAAGGAAACAA+CGG + chr7_1:39960278-39960297 None:intergenic 45.0%
!! ACAACAGCGTCGTTAGCTTT+CGG + chr7_1:39960325-39960344 None:intergenic 45.0%
CTTGATCAGCCTTACGCTTA+GGG + chr7_1:39960396-39960415 None:intergenic 45.0%
TCTTGATCAGCCTTACGCTT+AGG + chr7_1:39960397-39960416 None:intergenic 45.0%
AGTTCAGCAGAGAGAACAAG+AGG + chr7_1:39960424-39960443 None:intergenic 45.0%
AACAGCATCAGCAACAAGGA+AGG + chr7_1:39960464-39960483 None:intergenic 45.0%
CATCAACAGCATCAGCAACA+AGG + chr7_1:39960468-39960487 None:intergenic 45.0%
! AGTGTGAAGAGTGGTGGAAA+CGG - chr7_1:39961053-39961072 Msa1018960:intron 45.0%
CATTGAAGCTGCGATAGCAA+AGG - chr7_1:39961103-39961122 Msa1018960:intron 45.0%
GGAAAGGTGACTGATCCATA+CGG - chr7_1:39961197-39961216 Msa1018960:intron 45.0%
CTGATCCATACGGTTACGTT+TGG - chr7_1:39961207-39961226 Msa1018960:intron 45.0%
ACTCCTGCAAAGAAAGCTGT+TGG - chr7_1:39961239-39961258 Msa1018960:CDS 45.0%
TCACCAACAGCTTTCTTTGC+AGG + chr7_1:39961245-39961264 None:intergenic 45.0%
!! AAAGAAAGCTGTTGGTGACG+TGG - chr7_1:39961247-39961266 Msa1018960:CDS 45.0%
GGTGGTACTTTACTGCGTTA+TGG - chr7_1:39961479-39961498 Msa1018960:exon 45.0%
GGTACTTTACTGCGTTATGG+AGG - chr7_1:39961482-39961501 Msa1018960:exon 45.0%
!! AAAAAAATGAATAAATATTA+AGG + chr7_1:39961015-39961034 None:intergenic 5.0%
!! AAAAAAATGAATAAATATTA+AGG + chr7_1:39961015-39961034 None:intergenic 5.0%
CGGAAGCCATTGTTGAAGGA+AGG + chr7_1:39960216-39960235 None:intergenic 50.0%
!!! GGCACCGTTTTGAGGAGTTT+CGG + chr7_1:39960236-39960255 None:intergenic 50.0%
AACAACGGGAGAAGCGGTTT+CGG + chr7_1:39960263-39960282 None:intergenic 50.0%
GAAGGAAACAACGGGAGAAG+CGG + chr7_1:39960269-39960288 None:intergenic 50.0%
! GAACGCTTTTGCTGCTGAAG+AGG - chr7_1:39960354-39960373 Msa1018960:three_prime_UTR 50.0%
TGCGCTTAACCCTAAGCGTA+AGG - chr7_1:39960384-39960403 Msa1018960:three_prime_UTR 50.0%
TCTGCTGAACTCAAGATCGC+TGG - chr7_1:39960433-39960452 Msa1018960:exon 50.0%
TGGAACAGTGTGAAGAGTGG+TGG - chr7_1:39961047-39961066 Msa1018960:intron 50.0%
ATAGCAAAGGCAGTGAGTGC+TGG - chr7_1:39961116-39961135 Msa1018960:intron 50.0%
GGTGAAGTAGCTGAGTGTGA+AGG - chr7_1:39961152-39961171 Msa1018960:intron 50.0%
CGGAAGCCATTGTTGAAGGA+AGG + chr7_1:39960216-39960235 None:intergenic 50.0%
!!! GGCACCGTTTTGAGGAGTTT+CGG + chr7_1:39960236-39960255 None:intergenic 50.0%
AACAACGGGAGAAGCGGTTT+CGG + chr7_1:39960263-39960282 None:intergenic 50.0%
GAAGGAAACAACGGGAGAAG+CGG + chr7_1:39960269-39960288 None:intergenic 50.0%
! GAACGCTTTTGCTGCTGAAG+AGG - chr7_1:39960354-39960373 Msa1018960:three_prime_UTR 50.0%
TGCGCTTAACCCTAAGCGTA+AGG - chr7_1:39960384-39960403 Msa1018960:three_prime_UTR 50.0%
TCTGCTGAACTCAAGATCGC+TGG - chr7_1:39960433-39960452 Msa1018960:exon 50.0%
TGGAACAGTGTGAAGAGTGG+TGG - chr7_1:39961047-39961066 Msa1018960:intron 50.0%
ATAGCAAAGGCAGTGAGTGC+TGG - chr7_1:39961116-39961135 Msa1018960:intron 50.0%
GGTGAAGTAGCTGAGTGTGA+AGG - chr7_1:39961152-39961171 Msa1018960:intron 50.0%
! TCGGAAACGGCACCGTTTTG+AGG + chr7_1:39960244-39960263 None:intergenic 55.0%
GGGAGAAGCGGTTTCGGAAA+CGG + chr7_1:39960257-39960276 None:intergenic 55.0%
CTGAGGCTTGAAAGCGACGA+AGG + chr7_1:39960287-39960306 None:intergenic 55.0%
GGAGCTTCAACGAAGAGCTG+AGG + chr7_1:39960304-39960323 None:intergenic 55.0%
! AGTGCTGGAGCAGTTGCTGA+AGG - chr7_1:39961131-39961150 Msa1018960:intron 55.0%
!! GAAAGCTGTTGGTGACGTGG+AGG - chr7_1:39961250-39961269 Msa1018960:CDS 55.0%
! TCGGAAACGGCACCGTTTTG+AGG + chr7_1:39960244-39960263 None:intergenic 55.0%
GGGAGAAGCGGTTTCGGAAA+CGG + chr7_1:39960257-39960276 None:intergenic 55.0%
CTGAGGCTTGAAAGCGACGA+AGG + chr7_1:39960287-39960306 None:intergenic 55.0%
GGAGCTTCAACGAAGAGCTG+AGG + chr7_1:39960304-39960323 None:intergenic 55.0%
! AGTGCTGGAGCAGTTGCTGA+AGG - chr7_1:39961131-39961150 Msa1018960:intron 55.0%
!! GAAAGCTGTTGGTGACGTGG+AGG - chr7_1:39961250-39961269 Msa1018960:CDS 55.0%
! GCTGAGTGTGAAGGCGCGTG+TGG - chr7_1:39961161-39961180 Msa1018960:intron 65.0%
! GAGTGTGAAGGCGCGTGTGG+TGG - chr7_1:39961164-39961183 Msa1018960:intron 65.0%
! TGTGAAGGCGCGTGTGGTGG+CGG - chr7_1:39961167-39961186 Msa1018960:intron 65.0%
! GCTGAGTGTGAAGGCGCGTG+TGG - chr7_1:39961161-39961180 Msa1018960:intron 65.0%
! GAGTGTGAAGGCGCGTGTGG+TGG - chr7_1:39961164-39961183 Msa1018960:intron 65.0%
! TGTGAAGGCGCGTGTGGTGG+CGG - chr7_1:39961167-39961186 Msa1018960:intron 65.0%
! GTGAAGGCGCGTGTGGTGGC+GGG - chr7_1:39961168-39961187 Msa1018960:intron 70.0%
! GTGAAGGCGCGTGTGGTGGC+GGG - chr7_1:39961168-39961187 Msa1018960:intron 70.0%
!! TGGTGGCGGGCGCGTGGGAA+AGG - chr7_1:39961181-39961200 Msa1018960:intron 75.0%
!! TGGTGGCGGGCGCGTGGGAA+AGG - chr7_1:39961181-39961200 Msa1018960:intron 75.0%
!!! GCGTGTGGTGGCGGGCGCGT+GGG - chr7_1:39961176-39961195 Msa1018960:intron 80.0%
!!! GCGTGTGGTGGCGGGCGCGT+GGG - chr7_1:39961176-39961195 Msa1018960:intron 80.0%
!!! CGCGTGTGGTGGCGGGCGCG+TGG - chr7_1:39961175-39961194 Msa1018960:intron 85.0%
!!! CGCGTGTGGTGGCGGGCGCG+TGG - chr7_1:39961175-39961194 Msa1018960:intron 85.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 39960145 39961572 39960145 ID=Msa1018960;Name=Msa1018960
chr7_1 mRNA 39960145 39961572 39960145 ID=Msa1018960-mRNA-1;Parent=Msa1018960;Name=Msa1018960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=81|1|1|1|1|1|2|295|165
chr7_1 exon 39960145 39960662 39960145 ID=Msa1018960-mRNA-1:exon:7732;Parent=Msa1018960-mRNA-1
chr7_1 exon 39961217 39961572 39961217 ID=Msa1018960-mRNA-1:exon:7731;Parent=Msa1018960-mRNA-1
chr7_1 five_prime_UTR 39961492 39961572 39961492 ID=Msa1018960-mRNA-1:five_prime_utr;Parent=Msa1018960-mRNA-1
chr7_1 CDS 39961217 39961491 39961217 ID=Msa1018960-mRNA-1:cds;Parent=Msa1018960-mRNA-1
chr7_1 CDS 39960440 39960662 39960440 ID=Msa1018960-mRNA-1:cds;Parent=Msa1018960-mRNA-1
chr7_1 three_prime_UTR 39960145 39960439 39960145 ID=Msa1018960-mRNA-1:three_prime_utr;Parent=Msa1018960-mRNA-1
Gene Sequence

>Msa1018960

ATGGCTTCCGAAACTCCTCAAAACGGTGCCGTTTCCGAAACCGCTTCTCCCGTTGTTTCCTTCGTCGCTTTCAAGCCTCAGCTCTTCGTTGAAGCTCCGAAAGCTAACGACGCTGTTGTGTTCTACAAGAACGCTTTTGCTGCTGAAGAGGTTTCTCGTGCGCTTAACCCTAAGCGTAAGGCTGATCAAGAGCTTCCTCTTGTTCTCTCTGCTGAACTCAAGATCGCTGGTGCTTCCTTCCTTGTTGCTGATGCTGTTGATGATTCCGCTAAACCTGTGAAGAGTGGTGGAAACGGTGTCGTTTTCTGTCTTGAATCTGAGAACATTGAAGCTGCGATAGCAAAGGCAGTGAGTGCTGGAGCAGTTGCTGAAGGTGAAGTAGCTGAGTGTGAAGGCGCGTGTGGTGGCGGGCGCGTGGGAAAGGTGACTGATCCATACGGTTACGTTTGGCAATTCTGCACTCCTGCAAAGAAAGCTGTTGGTGACGTGGAGGCTTGA
Protein sequence

>Msa1018960

MASETPQNGAVSETASPVVSFVAFKPQLFVEAPKANDAVVFYKNAFAAEEVSRALNPKRKADQELPLVLSAELKIAGASFLVADAVDDSAKPVKSGGNGVVFCLESENIEAAIAKAVSAGAVAEGEVAECEGACGGGRVGKVTDPYGYVWQFCTPAKKAVGDVEA*