AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0816230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0816230 MtrunA17_Chr5g0410931 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr5g0410951 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr8g0383851 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr8g0383781 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr8g0390361 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr2g0295191 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr7g0237661 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr4g0033041 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr7g0218971 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr4g0046301 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr8g0364801 100.000 136 0 0 72 207 1 136 1.47e-95 274
Msa0816230 MtrunA17_Chr4g0054161 97.059 136 4 0 72 207 1 136 2.15e-92 266
Msa0816230 MtrunA17_Chr8g0363741 97.059 136 4 0 72 207 1 136 2.15e-92 266
Msa0816230 MtrunA17_Chr1g0156021 97.059 136 4 0 72 207 1 136 2.15e-92 266
Msa0816230 MtrunA17_Chr4g0054151 97.059 136 4 0 72 207 4 139 2.21e-92 266
Msa0816230 MtrunA17_Chr8g0388121 96.324 136 5 0 72 207 1 136 7.11e-92 265
Msa0816230 MtrunA17_Chr2g0319141 89.706 136 14 0 72 207 1 136 1.67e-85 249
Msa0816230 MtrunA17_Chr2g0319151 89.706 136 14 0 72 207 1 136 1.67e-85 249
Msa0816230 MtrunA17_Chr2g0319131 85.616 146 11 1 72 207 1 146 8.84e-82 239
Msa0816230 MtrunA17_Chr2g0319121 84.559 136 19 2 72 206 1 135 1.53e-73 218
Msa0816230 MtrunA17_Chr7g0218961 86.607 112 15 0 96 207 2 113 1.83e-64 194
Msa0816230 MtrunA17_Chr7g0222991 67.164 134 43 1 72 205 10 142 2.47e-54 170
Msa0816230 MtrunA17_Chr5g0407311 67.241 116 35 2 72 187 1 113 4.43e-44 143
Msa0816230 MtrunA17_Chr7g0223001 57.343 143 46 6 72 205 10 146 1.73e-41 137
Msa0816230 MtrunA17_Chr7g0240791 100.000 66 0 0 72 137 1 66 5.11e-40 134
Msa0816230 MtrunA17_Chr8g0347231 55.102 98 43 1 108 204 28 125 4.77e-29 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0816230 AT1G09200.1 99.265 136 1 0 72 207 1 136 6.35e-95 273
Msa0816230 AT5G65360.1 99.265 136 1 0 72 207 1 136 6.35e-95 273
Msa0816230 AT5G10390.1 99.265 136 1 0 72 207 1 136 6.35e-95 273
Msa0816230 AT5G10400.1 99.265 136 1 0 72 207 1 136 6.35e-95 273
Msa0816230 AT3G27360.1 99.265 136 1 0 72 207 1 136 6.35e-95 273
Msa0816230 AT4G40030.2 85.366 164 18 1 50 207 1 164 1.76e-93 270
Msa0816230 AT4G40030.3 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT4G40030.1 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT4G40030.4 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT5G10980.1 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT4G40040.2 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT4G40040.3 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT4G40040.1 97.059 136 4 0 72 207 1 136 2.97e-92 266
Msa0816230 AT5G65350.1 94.853 136 7 0 72 207 1 136 3.54e-90 261
Msa0816230 AT1G75600.1 93.382 136 9 0 72 207 1 136 2.59e-88 256
Msa0816230 AT1G13370.1 93.382 136 9 0 72 207 1 136 9.34e-88 254
Msa0816230 AT1G19890.1 89.781 137 13 1 72 207 1 137 3.94e-83 243
Msa0816230 AT5G12910.1 71.111 135 34 2 72 206 1 130 2.38e-65 198
Msa0816230 AT1G01370.2 53.982 113 49 2 95 205 63 174 8.04e-30 108
Msa0816230 AT1G01370.1 53.982 113 49 2 95 205 63 174 8.04e-30 108
Msa0816230 AT1G01370.4 53.982 113 49 2 95 205 63 174 8.04e-30 108
Msa0816230 AT1G01370.3 53.982 113 49 2 95 205 63 174 8.04e-30 108

Find 57 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTGAGGGGAAGGGTATT+TGG 0.206841 5_3:+50449415 None:intergenic
CAATTTCTCTAACCAATCTT+TGG 0.237540 5_3:+50449041 None:intergenic
GAGAGGGAAATCTGAAGATT+TGG 0.273601 5_3:+50449334 None:intergenic
TGAAAATAAGAGGAAGTGTT+TGG 0.299623 5_3:+50449280 None:intergenic
TCACGAAGAGCGACTGTTCC+TGG 0.301860 5_3:+50449115 None:intergenic
TATAATGAAATTAATCCTAT+TGG 0.327110 5_3:-50449465 None:intergenic
GCAGCTGAAGCTTATCTTGT+TGG 0.346066 5_3:-50448959 Msa0816230:CDS
TTGAATATCCTTAGGCATAA+TGG 0.349539 5_3:+50448889 None:intergenic
AGAGGGAAATCTGAAGATTT+GGG 0.349815 5_3:+50449335 None:intergenic
AGCTGATTTCCGAGCGGCTT+TGG 0.356003 5_3:+50449177 None:intergenic
GAAGCTTCCTTTCCAAAGAT+TGG 0.376805 5_3:-50449053 Msa0816230:CDS
CGGAAATCAGCTCCGGCGAC+TGG 0.383526 5_3:-50449166 Msa0816230:CDS
GTGAAGCGAATGAAAATAAG+AGG 0.385762 5_3:+50449270 None:intergenic
GAAAATAAGAGGAAGTGTTT+GGG 0.397278 5_3:+50449281 None:intergenic
AAGATTTGGGAAGATGAGAT+TGG 0.409914 5_3:+50449348 None:intergenic
GAAGAATAGTGGCCGTTGAT+AGG 0.414889 5_3:+50449370 None:intergenic
GAGCGGAAACAGCGCTACTC+TGG 0.433957 5_3:+50448990 None:intergenic
TTGGTGGCGAGCTGTTTCCT+TGG 0.436325 5_3:+50449196 None:intergenic
TTCTTCACTCCTCCAGTCGC+CGG 0.441465 5_3:+50449154 None:intergenic
AAGCCCTCTCGCCTCTGATT+CGG 0.461144 5_3:+50448858 None:intergenic
AGGCATAATGGTAACTCTCT+TGG 0.481302 5_3:+50448901 None:intergenic
GGCATGAATAGCACAAAGAT+TGG 0.490509 5_3:+50448922 None:intergenic
TGATTTCCGAGCGGCTTTGG+TGG 0.495094 5_3:+50449180 None:intergenic
CGAGCAAGTTGAATATCCTT+AGG 0.498424 5_3:+50448881 None:intergenic
AGCCGCTCGGAAATCAGCTC+CGG 0.498921 5_3:-50449173 Msa0816230:CDS
AAACAGCGCTACTCTGGAAT+CGG 0.503171 5_3:+50448996 None:intergenic
TTATAGAGAGGAGAAGAATG+AGG 0.504712 5_3:+50449308 None:intergenic
AGATGAGATTGGAAGAATAG+TGG 0.527156 5_3:+50449359 None:intergenic
AGAGCACAGAGCTTCTCATA+AGG 0.533669 5_3:-50449075 Msa0816230:CDS
GGAGAAGAATGAGGAATGAG+AGG 0.533875 5_3:+50449317 None:intergenic
CAACTTGCTCGCCGAATCAG+AGG 0.534605 5_3:-50448869 Msa0816230:CDS
GGCATGTGAAAGTAACCAAT+AGG 0.535172 5_3:+50449450 None:intergenic
GAGAGTTACCATTATGCCTA+AGG 0.538018 5_3:-50448897 Msa0816230:CDS
GCTATAAGTTTCTGAGGGGA+AGG 0.539979 5_3:+50449407 None:intergenic
ATTGGTTAGAGAAATTGCTC+AGG 0.542546 5_3:-50449035 Msa0816230:CDS
TCTCTAACCAATCTTTGGAA+AGG 0.542941 5_3:+50449046 None:intergenic
AGCTCGCCACCAAAGCCGCT+CGG 0.547091 5_3:-50449186 Msa0816230:CDS
CTACGGCTATAAGTTTCTGA+GGG 0.569010 5_3:+50449402 None:intergenic
TTGGTTACTTTCACATGCCA+CGG 0.575110 5_3:-50449446 Msa0816230:CDS
TTCAGCTGCTTCTTGAAGAG+CGG 0.576635 5_3:+50448973 None:intergenic
TCTACGGCTATAAGTTTCTG+AGG 0.580456 5_3:+50449401 None:intergenic
GAGAAGAATGAGGAATGAGA+GGG 0.584507 5_3:+50449318 None:intergenic
GGTATTTGGATCGCGATCCG+TGG 0.593508 5_3:+50449429 None:intergenic
TCGCCGAATCAGAGGCGAGA+GGG 0.598725 5_3:-50448861 Msa0816230:CDS
CTATAAGTTTCTGAGGGGAA+GGG 0.616763 5_3:+50449408 None:intergenic
CAAACAGCAAGAAAATCCAC+CGG 0.620184 5_3:-50449229 Msa0816230:CDS
TAGATAACCATTCCTATCAA+CGG 0.627853 5_3:-50449382 Msa0816230:CDS
AAATCAGCTCCGGCGACTGG+AGG 0.630058 5_3:-50449163 Msa0816230:CDS
AAGCCACACAGATTCCGTCC+AGG 0.632383 5_3:-50449133 Msa0816230:CDS
CTCGCCGAATCAGAGGCGAG+AGG 0.633846 5_3:-50448862 Msa0816230:CDS
ATAGTGGCCGTTGATAGGAA+TGG 0.661463 5_3:+50449375 None:intergenic
GAAGAGCGACTGTTCCTGGA+CGG 0.668213 5_3:+50449119 None:intergenic
GTTCCTGGACGGAATCTGTG+TGG 0.677012 5_3:+50449130 None:intergenic
CCACCGGAGGAAAAGCACCA+AGG 0.683879 5_3:-50449213 Msa0816230:CDS
ACAGCAAGAAAATCCACCGG+AGG 0.684797 5_3:-50449226 Msa0816230:CDS
CGCCGGAGCTGATTTCCGAG+CGG 0.685230 5_3:+50449171 None:intergenic
TACGGCTATAAGTTTCTGAG+GGG 0.701189 5_3:+50449403 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATAGGAATGGTTATCTA+CGG + chr5_3:50448935-50448954 None:intergenic 30.0%
TAGATAACCATTCCTATCAA+CGG - chr5_3:50448935-50448954 Msa0816230:CDS 30.0%
!!! TGTTTGGGTTTTTTATAGAG+AGG + chr5_3:50449024-50449043 None:intergenic 30.0%
!! GAAAATAAGAGGAAGTGTTT+GGG + chr5_3:50449039-50449058 None:intergenic 30.0%
!! TGAAAATAAGAGGAAGTGTT+TGG + chr5_3:50449040-50449059 None:intergenic 30.0%
! TTTTCATTCGCTTCACAAAA+TGG - chr5_3:50449054-50449073 Msa0816230:CDS 30.0%
CAATTTCTCTAACCAATCTT+TGG + chr5_3:50449279-50449298 None:intergenic 30.0%
TTGAATATCCTTAGGCATAA+TGG + chr5_3:50449431-50449450 None:intergenic 30.0%
AGATGAGATTGGAAGAATAG+TGG + chr5_3:50448961-50448980 None:intergenic 35.0%
AAGATTTGGGAAGATGAGAT+TGG + chr5_3:50448972-50448991 None:intergenic 35.0%
AGAGGGAAATCTGAAGATTT+GGG + chr5_3:50448985-50449004 None:intergenic 35.0%
TTATAGAGAGGAGAAGAATG+AGG + chr5_3:50449012-50449031 None:intergenic 35.0%
GTGAAGCGAATGAAAATAAG+AGG + chr5_3:50449050-50449069 None:intergenic 35.0%
!!! GGATTTTCTTGCTGTTTGTT+TGG + chr5_3:50449086-50449105 None:intergenic 35.0%
! TCTCTAACCAATCTTTGGAA+AGG + chr5_3:50449274-50449293 None:intergenic 35.0%
ATTGGTTAGAGAAATTGCTC+AGG - chr5_3:50449282-50449301 Msa0816230:CDS 35.0%
GGCATGTGAAAGTAACCAAT+AGG + chr5_3:50448870-50448889 None:intergenic 40.0%
! TTGGTTACTTTCACATGCCA+CGG - chr5_3:50448871-50448890 Msa0816230:CDS 40.0%
! CTATAAGTTTCTGAGGGGAA+GGG + chr5_3:50448912-50448931 None:intergenic 40.0%
TACGGCTATAAGTTTCTGAG+GGG + chr5_3:50448917-50448936 None:intergenic 40.0%
CTACGGCTATAAGTTTCTGA+GGG + chr5_3:50448918-50448937 None:intergenic 40.0%
TCTACGGCTATAAGTTTCTG+AGG + chr5_3:50448919-50448938 None:intergenic 40.0%
GAGAGGGAAATCTGAAGATT+TGG + chr5_3:50448986-50449005 None:intergenic 40.0%
GAGAAGAATGAGGAATGAGA+GGG + chr5_3:50449002-50449021 None:intergenic 40.0%
CAAACAGCAAGAAAATCCAC+CGG - chr5_3:50449088-50449107 Msa0816230:CDS 40.0%
GAAGCTTCCTTTCCAAAGAT+TGG - chr5_3:50449264-50449283 Msa0816230:CDS 40.0%
GGCATGAATAGCACAAAGAT+TGG + chr5_3:50449398-50449417 None:intergenic 40.0%
AGGCATAATGGTAACTCTCT+TGG + chr5_3:50449419-50449438 None:intergenic 40.0%
GAGAGTTACCATTATGCCTA+AGG - chr5_3:50449420-50449439 Msa0816230:CDS 40.0%
! CGAGCAAGTTGAATATCCTT+AGG + chr5_3:50449439-50449458 None:intergenic 40.0%
!! TTTCTGAGGGGAAGGGTATT+TGG + chr5_3:50448905-50448924 None:intergenic 45.0%
! GCTATAAGTTTCTGAGGGGA+AGG + chr5_3:50448913-50448932 None:intergenic 45.0%
! ATAGTGGCCGTTGATAGGAA+TGG + chr5_3:50448945-50448964 None:intergenic 45.0%
GAAGAATAGTGGCCGTTGAT+AGG + chr5_3:50448950-50448969 None:intergenic 45.0%
GGAGAAGAATGAGGAATGAG+AGG + chr5_3:50449003-50449022 None:intergenic 45.0%
!!! TTTCCTTGGTGCTTTTCCTC+CGG + chr5_3:50449110-50449129 None:intergenic 45.0%
AGAGCACAGAGCTTCTCATA+AGG - chr5_3:50449242-50449261 Msa0816230:CDS 45.0%
!! AAACAGCGCTACTCTGGAAT+CGG + chr5_3:50449324-50449343 None:intergenic 45.0%
TTCAGCTGCTTCTTGAAGAG+CGG + chr5_3:50449347-50449366 None:intergenic 45.0%
GCAGCTGAAGCTTATCTTGT+TGG - chr5_3:50449358-50449377 Msa0816230:CDS 45.0%
ACAGCAAGAAAATCCACCGG+AGG - chr5_3:50449091-50449110 Msa0816230:CDS 50.0%
GGTATTTGGATCGCGATCCG+TGG + chr5_3:50448891-50448910 None:intergenic 55.0%
!! TTGGTGGCGAGCTGTTTCCT+TGG + chr5_3:50449124-50449143 None:intergenic 55.0%
! TGATTTCCGAGCGGCTTTGG+TGG + chr5_3:50449140-50449159 None:intergenic 55.0%
! AGCTGATTTCCGAGCGGCTT+TGG + chr5_3:50449143-50449162 None:intergenic 55.0%
TTCTTCACTCCTCCAGTCGC+CGG + chr5_3:50449166-50449185 None:intergenic 55.0%
AAGCCACACAGATTCCGTCC+AGG - chr5_3:50449184-50449203 Msa0816230:CDS 55.0%
GTTCCTGGACGGAATCTGTG+TGG + chr5_3:50449190-50449209 None:intergenic 55.0%
GAAGAGCGACTGTTCCTGGA+CGG + chr5_3:50449201-50449220 None:intergenic 55.0%
TCACGAAGAGCGACTGTTCC+TGG + chr5_3:50449205-50449224 None:intergenic 55.0%
! CAACTTGCTCGCCGAATCAG+AGG - chr5_3:50449448-50449467 Msa0816230:CDS 55.0%
AAGCCCTCTCGCCTCTGATT+CGG + chr5_3:50449462-50449481 None:intergenic 55.0%
!!! CCTTGGTGCTTTTCCTCCGG+TGG + chr5_3:50449107-50449126 None:intergenic 60.0%
!! CCACCGGAGGAAAAGCACCA+AGG - chr5_3:50449104-50449123 Msa0816230:CDS 60.0%
AGCCGCTCGGAAATCAGCTC+CGG - chr5_3:50449144-50449163 Msa0816230:CDS 60.0%
AAATCAGCTCCGGCGACTGG+AGG - chr5_3:50449154-50449173 Msa0816230:CDS 60.0%
GAGCGGAAACAGCGCTACTC+TGG + chr5_3:50449330-50449349 None:intergenic 60.0%
TCGCCGAATCAGAGGCGAGA+GGG - chr5_3:50449456-50449475 Msa0816230:CDS 60.0%
AGCTCGCCACCAAAGCCGCT+CGG - chr5_3:50449131-50449150 Msa0816230:CDS 65.0%
! CGCCGGAGCTGATTTCCGAG+CGG + chr5_3:50449149-50449168 None:intergenic 65.0%
CGGAAATCAGCTCCGGCGAC+TGG - chr5_3:50449151-50449170 Msa0816230:CDS 65.0%
CTCGCCGAATCAGAGGCGAG+AGG - chr5_3:50449455-50449474 Msa0816230:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 50448856 50449483 50448856 ID=Msa0816230;Name=Msa0816230
chr5_3 mRNA 50448856 50449483 50448856 ID=Msa0816230-mRNA-1;Parent=Msa0816230;Name=Msa0816230-mRNA-1;_AED=0.18;_eAED=0.23;_QI=0|0|0|1|0|0|2|0|207
chr5_3 exon 50449406 50449483 50449406 ID=Msa0816230-mRNA-1:exon:15152;Parent=Msa0816230-mRNA-1
chr5_3 exon 50448856 50449401 50448856 ID=Msa0816230-mRNA-1:exon:15151;Parent=Msa0816230-mRNA-1
chr5_3 CDS 50449406 50449483 50449406 ID=Msa0816230-mRNA-1:cds;Parent=Msa0816230-mRNA-1
chr5_3 CDS 50448856 50449401 50448856 ID=Msa0816230-mRNA-1:cds;Parent=Msa0816230-mRNA-1
Gene Sequence

>Msa0816230

ATGAAATTAATCCTATTGGTTACTTTCACATGCCACGGATCGCGATCCAAATACCCTTCCCCTCAGAAACTTATAGCCATAACCATTCCTATCAACGGCCACTATTCTTCCAATCTCATCTTCCCAAATCTTCAGATTTCCCTCTCATTCCTCATTCTTCTCCTCTCTATAAAAAACCCAAACACTTCCTCTTATTTTCATTCGCTTCACAAAATGGCACGTACCAAACAAACAGCAAGAAAATCCACCGGAGGAAAAGCACCAAGGAAACAGCTCGCCACCAAAGCCGCTCGGAAATCAGCTCCGGCGACTGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACAGTCGCTCTTCGTGAAATCAGAAAGTATCAGAAGAGCACAGAGCTTCTCATAAGGAAGCTTCCTTTCCAAAGATTGGTTAGAGAAATTGCTCAGGATTTCAAAACTGATCTCCGATTCCAGAGTAGCGCTGTTTCCGCTCTTCAAGAAGCAGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACCAATCTTTGTGCTATTCATGCCAAGAGAGTTACCATTATGCCTAAGGATATTCAACTTGCTCGCCGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>Msa0816230

MKLILLVTFTCHGSRSKYPSPQKLIAITIPINGHYSSNLIFPNLQISLSFLILLLSIKNPNTSSYFHSLHKMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*