Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737140 | VAH18507.1 | 100.000 | 137 | 0 | 0 | 1 | 137 | 1 | 137 | 1.56e-93 | 277 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737140 | sp|P68428|H32_WHEAT | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.51e-95 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737140 | A0A446JZI6 | 100.000 | 137 | 0 | 0 | 1 | 137 | 1 | 137 | 7.45e-94 | 277 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0027800 | Msa0737140 | 0.800174 | 1.630772e-48 | -8.615850e-47 |
| Msa0030070 | Msa0737140 | 0.833675 | 4.654461e-56 | -8.615850e-47 |
| Msa0050530 | Msa0737140 | 0.825294 | 5.070525e-54 | -8.615850e-47 |
| Msa0090440 | Msa0737140 | 0.811127 | 8.178926e-51 | -8.615850e-47 |
| Msa0091350 | Msa0737140 | 0.813074 | 3.076331e-51 | -8.615850e-47 |
| Msa0100600 | Msa0737140 | 0.823426 | 1.394013e-53 | -8.615850e-47 |
| Msa0100890 | Msa0737140 | 0.815378 | 9.530968e-52 | -8.615850e-47 |
| Msa0106630 | Msa0737140 | 0.810418 | 1.163982e-50 | -8.615850e-47 |
| Msa0615630 | Msa0737140 | 0.803282 | 3.755563e-49 | -8.615850e-47 |
| Msa0618990 | Msa0737140 | 0.803057 | 4.180227e-49 | -8.615850e-47 |
| Msa0620500 | Msa0737140 | 0.819629 | 1.050563e-52 | -8.615850e-47 |
| Msa0640800 | Msa0737140 | 0.810806 | 9.595544e-51 | -8.615850e-47 |
| Msa0645340 | Msa0737140 | 0.803669 | 3.121926e-49 | -8.615850e-47 |
| Msa0647440 | Msa0737140 | 0.820155 | 7.963415e-53 | -8.615850e-47 |
| Msa0648760 | Msa0737140 | 0.806073 | 9.816644e-50 | -8.615850e-47 |
| Msa0659200 | Msa0737140 | 0.820274 | 7.480574e-53 | -8.615850e-47 |
| Msa0663290 | Msa0737140 | 0.823319 | 1.476593e-53 | -8.615850e-47 |
| Msa0664070 | Msa0737140 | 0.830941 | 2.210952e-55 | -8.615850e-47 |
| Msa0684750 | Msa0737140 | 0.822138 | 2.780071e-53 | -8.615850e-47 |
| Msa0688620 | Msa0737140 | 0.806434 | 8.240852e-50 | -8.615850e-47 |
| Msa0705140 | Msa0737140 | 0.815196 | 1.046305e-51 | -8.615850e-47 |
| Msa0705180 | Msa0737140 | 0.830834 | 2.349237e-55 | -8.615850e-47 |
| Msa0707260 | Msa0737140 | 0.812619 | 3.870799e-51 | -8.615850e-47 |
| Msa0708980 | Msa0737140 | 0.805814 | 1.113252e-49 | -8.615850e-47 |
| Msa0717900 | Msa0737140 | 0.825679 | 4.110874e-54 | -8.615850e-47 |
| Msa0720280 | Msa0737140 | 0.845693 | 3.463400e-59 | -8.615850e-47 |
| Msa0722700 | Msa0737140 | 0.804648 | 1.952839e-49 | -8.615850e-47 |
| Msa0737130 | Msa0737140 | 0.821544 | 3.816268e-53 | -8.615850e-47 |
| Msa0737140 | Msa0749190 | 0.807670 | 4.511683e-50 | -8.615850e-47 |
| Msa0737140 | Msa0750520 | 0.810345 | 1.207074e-50 | -8.615850e-47 |
| Msa0737140 | Msa0751720 | 0.818235 | 2.179518e-52 | -8.615850e-47 |
| Msa0737140 | Msa0778600 | 0.815337 | 9.733885e-52 | -8.615850e-47 |
| Msa0737140 | Msa0803140 | 0.854590 | 1.112613e-61 | -8.615850e-47 |
| Msa0737140 | Msa0816080 | 0.819482 | 1.135148e-52 | -8.615850e-47 |
| Msa0737140 | Msa0816230 | 0.844942 | 5.531213e-59 | -8.615850e-47 |
| Msa0737140 | Msa0837680 | 0.847629 | 1.024561e-59 | -8.615850e-47 |
| Msa0737140 | Msa0841500 | 0.843538 | 1.317330e-58 | -8.615850e-47 |
| Msa0737140 | Msa0846900 | 0.809931 | 1.482444e-50 | -8.615850e-47 |
| Msa0737140 | Msa0856610 | 0.884719 | 1.507827e-71 | -8.615850e-47 |
| Msa0737140 | Msa0861440 | 0.818723 | 1.689429e-52 | -8.615850e-47 |
| Msa0737140 | Msa0900090 | 0.827064 | 1.922769e-54 | -8.615850e-47 |
| Msa0737140 | Msa0926310 | 0.832019 | 1.200349e-55 | -8.615850e-47 |
| Msa0737140 | Msa0930040 | 0.807408 | 5.128122e-50 | -8.615850e-47 |
| Msa0737140 | Msa1000780 | 0.838950 | 2.120902e-57 | -8.615850e-47 |
| Msa0737140 | Msa1008670 | 0.827166 | 1.817667e-54 | -8.615850e-47 |
| Msa0737140 | Msa1018960 | 0.811917 | 5.507002e-51 | -8.615850e-47 |
| Msa0737140 | Msa1071410 | 0.811183 | 7.953953e-51 | -8.615850e-47 |
| Msa0737140 | Msa1076020 | 0.802771 | 4.788370e-49 | -8.615850e-47 |
| Msa0737140 | Msa1078860 | 0.831321 | 1.784004e-55 | -8.615850e-47 |
| Msa0737140 | Msa1078990 | 0.836257 | 1.040505e-56 | -8.615850e-47 |
| Msa0737140 | Msa1085680 | 0.800876 | 1.173059e-48 | -8.615850e-47 |
| Msa0737140 | Msa1144990 | 0.817651 | 2.953088e-52 | -8.615850e-47 |
| Msa0737140 | Msa1147560 | 0.817210 | 3.711208e-52 | -8.615850e-47 |
| Msa0737140 | Msa1160930 | 0.802296 | 6.000833e-49 | -8.615850e-47 |
| Msa0737140 | Msa1163180 | 0.826617 | 2.459485e-54 | -8.615850e-47 |
| Msa0737140 | Msa1166820 | 0.801252 | 9.827415e-49 | -8.615850e-47 |
| Msa0737140 | Msa1169170 | 0.814856 | 1.245156e-51 | -8.615850e-47 |
| Msa0737140 | Msa1169690 | 0.827439 | 1.563993e-54 | -8.615850e-47 |
| Msa0737140 | Msa1215980 | 0.820803 | 5.654214e-53 | -8.615850e-47 |
| Msa0737140 | Msa1254420 | 0.807322 | 5.350232e-50 | -8.615850e-47 |
| Msa0737140 | Msa1263740 | 0.800096 | 1.692100e-48 | -8.615850e-47 |
| Msa0737140 | Msa1294790 | 0.821624 | 3.657219e-53 | -8.615850e-47 |
| Msa0737140 | Msa1339230 | 0.862007 | 6.871903e-64 | -8.615850e-47 |
| Msa0737140 | Msa1381470 | 0.804761 | 1.849727e-49 | -8.615850e-47 |
| Msa0737140 | Msa1381800 | 0.808891 | 2.478353e-50 | -8.615850e-47 |
| Msa0737140 | Msa1387500 | 0.853100 | 2.986577e-61 | -8.615850e-47 |
| Msa0737140 | Msa1390210 | 0.832173 | 1.099197e-55 | -8.615850e-47 |
| Msa0737140 | Msa1393560 | 0.804129 | 2.505068e-49 | -8.615850e-47 |
| Msa0737140 | Msa1399190 | 0.829421 | 5.196484e-55 | -8.615850e-47 |
| Msa0737140 | Msa1406720 | 0.816511 | 5.326054e-52 | -8.615850e-47 |
| Msa0737140 | Msa1410190 | 0.858423 | 8.323313e-63 | -8.615850e-47 |
| Msa0737140 | Msa1426320 | 0.815512 | 8.900591e-52 | -8.615850e-47 |
| Msa0737140 | Msa1435100 | 0.826565 | 2.529919e-54 | -8.615850e-47 |
| Msa0737140 | Msa1439730 | 0.833389 | 5.484028e-56 | -8.615850e-47 |
| Msa0125590 | Msa0737140 | 0.838709 | 2.448220e-57 | -8.615850e-47 |
| Msa0127340 | Msa0737140 | 0.820717 | 5.919655e-53 | -8.615850e-47 |
| Msa0158900 | Msa0737140 | 0.808617 | 2.836484e-50 | -8.615850e-47 |
| Msa0170070 | Msa0737140 | 0.855231 | 7.248286e-62 | -8.615850e-47 |
| Msa0186180 | Msa0737140 | 0.817907 | 2.584589e-52 | -8.615850e-47 |
| Msa0186360 | Msa0737140 | 0.802980 | 4.335884e-49 | -8.615850e-47 |
| Msa0204970 | Msa0737140 | 0.824914 | 6.234504e-54 | -8.615850e-47 |
| Msa0225800 | Msa0737140 | 0.830838 | 2.343496e-55 | -8.615850e-47 |
| Msa0373170 | Msa0737140 | 0.821656 | 3.595135e-53 | -8.615850e-47 |
| Msa0382230 | Msa0737140 | 0.837482 | 5.065767e-57 | -8.615850e-47 |
| Msa0383940 | Msa0737140 | 0.802443 | 5.597213e-49 | -8.615850e-47 |
| Msa0415820 | Msa0737140 | 0.821784 | 3.358683e-53 | -8.615850e-47 |
| Msa0421750 | Msa0737140 | 0.823742 | 1.175673e-53 | -8.615850e-47 |
| Msa0429970 | Msa0737140 | 0.812698 | 3.720050e-51 | -8.615850e-47 |
| Msa0433630 | Msa0737140 | 0.840027 | 1.113254e-57 | -8.615850e-47 |
| Msa0433760 | Msa0737140 | 0.807870 | 4.092854e-50 | -8.615850e-47 |
| Msa0466520 | Msa0737140 | 0.803651 | 3.148805e-49 | -8.615850e-47 |
| Msa0467690 | Msa0737140 | 0.822559 | 2.220137e-53 | -8.615850e-47 |
| Msa0477630 | Msa0737140 | 0.805635 | 1.213724e-49 | -8.615850e-47 |
| Msa0478140 | Msa0737140 | 0.811025 | 8.606227e-51 | -8.615850e-47 |
| Msa0478200 | Msa0737140 | 0.805328 | 1.407366e-49 | -8.615850e-47 |
| Msa0249870 | Msa0737140 | 0.806173 | 9.352509e-50 | -8.615850e-47 |
| Msa0260630 | Msa0737140 | 0.808036 | 3.773004e-50 | -8.615850e-47 |
| Msa0281540 | Msa0737140 | 0.803585 | 3.249254e-49 | -8.615850e-47 |
| Msa0320250 | Msa0737140 | 0.829822 | 4.151546e-55 | -8.615850e-47 |
| Msa0320270 | Msa0737140 | 0.827256 | 1.730072e-54 | -8.615850e-47 |
| Msa0320290 | Msa0737140 | 0.837477 | 5.080717e-57 | -8.615850e-47 |
| Msa0320580 | Msa0737140 | 0.810476 | 1.131349e-50 | -8.615850e-47 |
| Msa0344110 | Msa0737140 | 0.813700 | 2.241274e-51 | -8.615850e-47 |
| Msa0506880 | Msa0737140 | 0.827901 | 1.211072e-54 | -8.615850e-47 |
| Msa0507810 | Msa0737140 | 0.816049 | 6.755756e-52 | -8.615850e-47 |
| Msa0507920 | Msa0737140 | 0.808938 | 2.422072e-50 | -8.615850e-47 |
| Msa0514020 | Msa0737140 | 0.827014 | 1.976054e-54 | -8.615850e-47 |
| Msa0520680 | Msa0737140 | 0.836549 | 8.765595e-57 | -8.615850e-47 |
| Msa0521510 | Msa0737140 | 0.806249 | 9.015683e-50 | -8.615850e-47 |
| Msa0521530 | Msa0737140 | 0.810332 | 1.214773e-50 | -8.615850e-47 |
| Msa0521680 | Msa0737140 | 0.805118 | 1.557331e-49 | -8.615850e-47 |
| Msa0527850 | Msa0737140 | 0.846876 | 1.648491e-59 | -8.615850e-47 |
| Msa0544410 | Msa0737140 | 0.810774 | 9.753176e-51 | -8.615850e-47 |
| Msa0544430 | Msa0737140 | 0.805878 | 1.079091e-49 | -8.615850e-47 |
| Msa0554300 | Msa0737140 | 0.831366 | 1.738942e-55 | -8.615850e-47 |
| Msa0561560 | Msa0737140 | 0.802141 | 6.459360e-49 | -8.615850e-47 |
| Msa0572830 | Msa0737140 | 0.828223 | 1.013198e-54 | -8.615850e-47 |
| Msa0576330 | Msa0737140 | 0.804207 | 2.413359e-49 | -8.615850e-47 |
| Msa0582460 | Msa0737140 | 0.841922 | 3.542421e-58 | -8.615850e-47 |
| Msa0584530 | Msa0737140 | 0.804485 | 2.112426e-49 | -8.615850e-47 |
| Msa0586200 | Msa0737140 | 0.828348 | 9.453512e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737140 | MtrunA17_Chr5g0410931 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr5g0410951 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr8g0383851 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr8g0383781 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr8g0390361 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr2g0295191 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr7g0237661 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr4g0033041 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr7g0218971 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr4g0046301 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr8g0364801 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.59e-96 | 273 |
| Msa0737140 | MtrunA17_Chr4g0054151 | 97.059 | 136 | 4 | 0 | 1 | 136 | 4 | 139 | 2.67e-93 | 265 |
| Msa0737140 | MtrunA17_Chr4g0054161 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.43e-93 | 265 |
| Msa0737140 | MtrunA17_Chr8g0363741 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.43e-93 | 265 |
| Msa0737140 | MtrunA17_Chr1g0156021 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.43e-93 | 265 |
| Msa0737140 | MtrunA17_Chr8g0388121 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.68e-92 | 263 |
| Msa0737140 | MtrunA17_Chr2g0319141 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.16e-86 | 249 |
| Msa0737140 | MtrunA17_Chr2g0319151 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.16e-86 | 249 |
| Msa0737140 | MtrunA17_Chr2g0319131 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.80e-82 | 238 |
| Msa0737140 | MtrunA17_Chr2g0319121 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 5.63e-75 | 219 |
| Msa0737140 | MtrunA17_Chr7g0218961 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 7.01e-66 | 195 |
| Msa0737140 | MtrunA17_Chr7g0222991 | 67.164 | 134 | 43 | 1 | 1 | 134 | 10 | 142 | 8.00e-56 | 171 |
| Msa0737140 | MtrunA17_Chr5g0407311 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.49e-45 | 144 |
| Msa0737140 | MtrunA17_Chr7g0223001 | 58.647 | 133 | 41 | 3 | 3 | 134 | 27 | 146 | 6.38e-43 | 139 |
| Msa0737140 | MtrunA17_Chr7g0240791 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 1.02e-40 | 133 |
| Msa0737140 | MtrunA17_Chr8g0347231 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 3.92e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737140 | AT1G09200.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.57e-95 | 271 |
| Msa0737140 | AT5G65360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.57e-95 | 271 |
| Msa0737140 | AT5G10390.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.57e-95 | 271 |
| Msa0737140 | AT5G10400.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.57e-95 | 271 |
| Msa0737140 | AT3G27360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.57e-95 | 271 |
| Msa0737140 | AT4G40030.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT4G40030.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT4G40030.4 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT5G10980.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT4G40040.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT4G40040.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT4G40040.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 4.73e-93 | 265 |
| Msa0737140 | AT4G40030.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 8.42e-93 | 265 |
| Msa0737140 | AT5G65350.1 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 6.62e-91 | 260 |
| Msa0737140 | AT1G75600.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.98e-89 | 256 |
| Msa0737140 | AT1G13370.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 7.40e-89 | 254 |
| Msa0737140 | AT1G19890.1 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.04e-83 | 241 |
| Msa0737140 | AT5G12910.1 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 1.12e-66 | 198 |
| Msa0737140 | AT1G01370.2 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 3.68e-31 | 110 |
| Msa0737140 | AT1G01370.1 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 3.68e-31 | 110 |
| Msa0737140 | AT1G01370.4 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 3.68e-31 | 110 |
| Msa0737140 | AT1G01370.3 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 3.68e-31 | 110 |
Find 33 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTTCTCTAACCAATCTT+TGG | 0.237540 | 5_1:+54134539 | None:intergenic |
| TTCTTCACTCCTCCGGTTGC+CGG | 0.280449 | 5_1:+54134652 | None:intergenic |
| TCACGAAGAGCGACTGTTCC+TGG | 0.301860 | 5_1:+54134613 | None:intergenic |
| GCAGCTGAAGCTTATCTTGT+TGG | 0.346066 | 5_1:-54134457 | Msa0737140:CDS |
| AGCTGATTTCCGAGCGGCTT+TGG | 0.356003 | 5_1:+54134675 | None:intergenic |
| GAAGCTTCCTTTCCAAAGAT+TGG | 0.376805 | 5_1:-54134551 | Msa0737140:CDS |
| CGGAAATCAGCTCCGGCAAC+CGG | 0.396553 | 5_1:-54134664 | Msa0737140:CDS |
| GAGCGGAAACAGCGCTACTC+TGG | 0.433957 | 5_1:+54134488 | None:intergenic |
| TTGGTGGCGAGCTGTTTCCT+TGG | 0.436325 | 5_1:+54134694 | None:intergenic |
| AAGCCCTCTCGCCTCTGATT+CGG | 0.477567 | 5_1:+54134356 | None:intergenic |
| ATGTGGCTTCTTCACTCCTC+CGG | 0.485740 | 5_1:+54134645 | None:intergenic |
| AGCCGCTCGGAAATCAGCTC+CGG | 0.492100 | 5_1:-54134671 | Msa0737140:CDS |
| AGCATGAATAGCACAAAGAT+TGG | 0.492949 | 5_1:+54134420 | None:intergenic |
| TGATTTCCGAGCGGCTTTGG+TGG | 0.495094 | 5_1:+54134678 | None:intergenic |
| CGAGCAAGTTGAATATCCTT+AGG | 0.498424 | 5_1:+54134379 | None:intergenic |
| AAACAGCGCTACTCTGGAAT+CGG | 0.503171 | 5_1:+54134494 | None:intergenic |
| GAGAGTTACTATTATGCCTA+AGG | 0.517685 | 5_1:-54134395 | Msa0737140:CDS |
| AGAGCACAGAGCTTCTCATA+AGG | 0.533669 | 5_1:-54134573 | Msa0737140:CDS |
| CAACTTGCTCGCCGAATCAG+AGG | 0.534605 | 5_1:-54134367 | Msa0737140:CDS |
| ATTGGTTAGAGAAATTGCTC+AGG | 0.542546 | 5_1:-54134533 | Msa0737140:CDS |
| TCTCTAACCAATCTTTGGAA+AGG | 0.542941 | 5_1:+54134544 | None:intergenic |
| AGCTCGCCACCAAAGCCGCT+CGG | 0.547091 | 5_1:-54134684 | Msa0737140:CDS |
| TTCAGCTGCTTCTTGAAGAG+CGG | 0.576635 | 5_1:+54134471 | None:intergenic |
| TCGCCGAATCAGAGGCGAGA+GGG | 0.598725 | 5_1:-54134359 | Msa0737140:CDS |
| AAATCAGCTCCGGCAACCGG+AGG | 0.609979 | 5_1:-54134661 | Msa0737140:CDS |
| AAGCCACATAGATTCCGTCC+AGG | 0.611045 | 5_1:-54134631 | Msa0737140:CDS |
| CAAACAGCAAGAAAATCCAC+CGG | 0.620184 | 5_1:-54134727 | Msa0737140:CDS |
| CTCGCCGAATCAGAGGCGAG+AGG | 0.633846 | 5_1:-54134360 | Msa0737140:CDS |
| GTTCCTGGACGGAATCTATG+TGG | 0.636510 | 5_1:+54134628 | None:intergenic |
| GAAGAGCGACTGTTCCTGGA+CGG | 0.668213 | 5_1:+54134617 | None:intergenic |
| CCACCGGAGGAAAAGCACCA+AGG | 0.683879 | 5_1:-54134711 | Msa0737140:CDS |
| ACAGCAAGAAAATCCACCGG+AGG | 0.684797 | 5_1:-54134724 | Msa0737140:CDS |
| TGCCGGAGCTGATTTCCGAG+CGG | 0.724331 | 5_1:+54134669 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAATTTCTCTAACCAATCTT+TGG | + | chr5_1:54134551-54134570 | None:intergenic | 30.0% | |
| !!! | GGATTTTCTTGCTGTTTGTT+TGG | + | chr5_1:54134358-54134377 | None:intergenic | 35.0% |
| ! | TCTCTAACCAATCTTTGGAA+AGG | + | chr5_1:54134546-54134565 | None:intergenic | 35.0% |
| ATTGGTTAGAGAAATTGCTC+AGG | - | chr5_1:54134554-54134573 | Msa0737140:CDS | 35.0% | |
| AGCATGAATAGCACAAAGAT+TGG | + | chr5_1:54134670-54134689 | None:intergenic | 35.0% | |
| GAGAGTTACTATTATGCCTA+AGG | - | chr5_1:54134692-54134711 | Msa0737140:CDS | 35.0% | |
| CAAACAGCAAGAAAATCCAC+CGG | - | chr5_1:54134360-54134379 | Msa0737140:CDS | 40.0% | |
| GAAGCTTCCTTTCCAAAGAT+TGG | - | chr5_1:54134536-54134555 | Msa0737140:CDS | 40.0% | |
| ! | CGAGCAAGTTGAATATCCTT+AGG | + | chr5_1:54134711-54134730 | None:intergenic | 40.0% |
| !!! | TTTCCTTGGTGCTTTTCCTC+CGG | + | chr5_1:54134382-54134401 | None:intergenic | 45.0% |
| AGAGCACAGAGCTTCTCATA+AGG | - | chr5_1:54134514-54134533 | Msa0737140:CDS | 45.0% | |
| !! | AAACAGCGCTACTCTGGAAT+CGG | + | chr5_1:54134596-54134615 | None:intergenic | 45.0% |
| TTCAGCTGCTTCTTGAAGAG+CGG | + | chr5_1:54134619-54134638 | None:intergenic | 45.0% | |
| GCAGCTGAAGCTTATCTTGT+TGG | - | chr5_1:54134630-54134649 | Msa0737140:CDS | 45.0% | |
| ACAGCAAGAAAATCCACCGG+AGG | - | chr5_1:54134363-54134382 | Msa0737140:CDS | 50.0% | |
| ATGTGGCTTCTTCACTCCTC+CGG | + | chr5_1:54134445-54134464 | None:intergenic | 50.0% | |
| AAGCCACATAGATTCCGTCC+AGG | - | chr5_1:54134456-54134475 | Msa0737140:CDS | 50.0% | |
| GTTCCTGGACGGAATCTATG+TGG | + | chr5_1:54134462-54134481 | None:intergenic | 50.0% | |
| !! | TTGGTGGCGAGCTGTTTCCT+TGG | + | chr5_1:54134396-54134415 | None:intergenic | 55.0% |
| ! | TGATTTCCGAGCGGCTTTGG+TGG | + | chr5_1:54134412-54134431 | None:intergenic | 55.0% |
| ! | AGCTGATTTCCGAGCGGCTT+TGG | + | chr5_1:54134415-54134434 | None:intergenic | 55.0% |
| TTCTTCACTCCTCCGGTTGC+CGG | + | chr5_1:54134438-54134457 | None:intergenic | 55.0% | |
| GAAGAGCGACTGTTCCTGGA+CGG | + | chr5_1:54134473-54134492 | None:intergenic | 55.0% | |
| TCACGAAGAGCGACTGTTCC+TGG | + | chr5_1:54134477-54134496 | None:intergenic | 55.0% | |
| ! | CAACTTGCTCGCCGAATCAG+AGG | - | chr5_1:54134720-54134739 | Msa0737140:CDS | 55.0% |
| AAGCCCTCTCGCCTCTGATT+CGG | + | chr5_1:54134734-54134753 | None:intergenic | 55.0% | |
| !!! | CCTTGGTGCTTTTCCTCCGG+TGG | + | chr5_1:54134379-54134398 | None:intergenic | 60.0% |
| !! | CCACCGGAGGAAAAGCACCA+AGG | - | chr5_1:54134376-54134395 | Msa0737140:CDS | 60.0% |
| AGCCGCTCGGAAATCAGCTC+CGG | - | chr5_1:54134416-54134435 | Msa0737140:CDS | 60.0% | |
| ! | TGCCGGAGCTGATTTCCGAG+CGG | + | chr5_1:54134421-54134440 | None:intergenic | 60.0% |
| CGGAAATCAGCTCCGGCAAC+CGG | - | chr5_1:54134423-54134442 | Msa0737140:CDS | 60.0% | |
| AAATCAGCTCCGGCAACCGG+AGG | - | chr5_1:54134426-54134445 | Msa0737140:CDS | 60.0% | |
| GAGCGGAAACAGCGCTACTC+TGG | + | chr5_1:54134602-54134621 | None:intergenic | 60.0% | |
| TCGCCGAATCAGAGGCGAGA+GGG | - | chr5_1:54134728-54134747 | Msa0737140:CDS | 60.0% | |
| AGCTCGCCACCAAAGCCGCT+CGG | - | chr5_1:54134403-54134422 | Msa0737140:CDS | 65.0% | |
| CTCGCCGAATCAGAGGCGAG+AGG | - | chr5_1:54134727-54134746 | Msa0737140:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 54134345 | 54134764 | 54134345 | ID=Msa0737140;Name=Msa0737140 |
| chr5_1 | mRNA | 54134345 | 54134764 | 54134345 | ID=Msa0737140-mRNA-1;Parent=Msa0737140;Name=Msa0737140-mRNA-1;_AED=0.36;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|139 |
| chr5_1 | exon | 54134345 | 54134764 | 54134345 | ID=Msa0737140-mRNA-1:exon:15239;Parent=Msa0737140-mRNA-1 |
| chr5_1 | CDS | 54134345 | 54134764 | 54134345 | ID=Msa0737140-mRNA-1:cds;Parent=Msa0737140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
