AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1339230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1339230 MtrunA17_Chr4g0035621 98.026 152 1 1 1 150 1 152 7.74e-101 285
Msa1339230 MtrunA17_Chr2g0327951 87.333 150 17 1 1 150 38 185 6.83e-84 244
Msa1339230 MtrunA17_Chr4g0031651 95.333 150 5 1 1 150 1 148 1.95e-83 241
Msa1339230 MtrunA17_Chr4g0031001 94.667 150 6 1 1 150 1 148 5.29e-83 240
Msa1339230 MtrunA17_Chr2g0327931 91.558 154 8 2 1 150 1 153 4.45e-82 238
Msa1339230 MtrunA17_Chr4g0031041 93.182 132 8 1 20 150 14 145 1.03e-81 237
Msa1339230 MtrunA17_Chr4g0032301 93.333 150 8 1 1 150 1 148 2.35e-80 234
Msa1339230 MtrunA17_Chr8g0379311 96.212 132 5 0 1 132 1 132 3.37e-75 221
Msa1339230 MtrunA17_Chr7g0272611 79.699 133 26 1 10 142 13 144 3.95e-71 210
Msa1339230 MtrunA17_Chr7g0268831 75.000 128 26 3 4 130 6 128 5.98e-60 182
Msa1339230 MtrunA17_Chr7g0273351 74.219 128 29 1 3 130 2 125 6.20e-60 182
Msa1339230 MtrunA17_Chr7g0259151 73.228 127 31 1 4 130 5 128 7.36e-60 181
Msa1339230 MtrunA17_Chr2g0319191 70.543 129 38 0 11 139 3 131 2.53e-59 180
Msa1339230 MtrunA17_Chr7g0273331 74.219 128 29 1 3 130 58 181 3.63e-59 182
Msa1339230 MtrunA17_Chr2g0319181 73.950 119 31 0 11 129 3 121 1.31e-57 176
Msa1339230 MtrunA17_Chr6g0449661 62.992 127 33 3 24 149 85 198 7.88e-47 150
Msa1339230 MtrunA17_Chr3g0119971 62.698 126 33 3 25 149 81 193 1.89e-46 149
Msa1339230 MtrunA17_Chr4g0031031 75.238 105 21 1 45 149 10 109 2.63e-46 146
Msa1339230 MtrunA17_Chr7g0273341 71.296 108 20 2 23 130 57 153 4.56e-45 145
Msa1339230 MtrunA17_Chr1g0148961 60.714 112 42 2 21 131 23 133 2.82e-36 121
Msa1339230 MtrunA17_Chr1g0148951 60.714 112 42 2 21 131 23 133 2.82e-36 121
Msa1339230 MtrunA17_Chr1g0211651 60.550 109 41 2 21 128 20 127 2.29e-35 119
Msa1339230 MtrunA17_Chr1g0184901 62.500 104 37 2 26 128 28 130 1.21e-34 117
Msa1339230 MtrunA17_Chr1g0183701 58.904 73 30 0 67 139 30 102 5.14e-25 92.4
Msa1339230 MtrunA17_Chr4g0024701 50.476 105 32 2 24 128 26 110 1.94e-22 85.9
Msa1339230 MtrunA17_Chr4g0000531 71.111 45 13 0 85 129 5 49 1.07e-15 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1339230 AT5G02560.1 79.739 153 28 1 1 150 1 153 1.54e-77 227
Msa1339230 AT5G27670.1 76.316 152 32 3 1 150 1 150 3.46e-74 218
Msa1339230 AT5G02560.2 68.927 177 28 2 1 150 1 177 1.11e-72 216
Msa1339230 AT5G59870.1 75.000 148 36 1 4 150 3 150 9.35e-72 212
Msa1339230 AT1G51060.1 76.667 120 28 0 11 130 3 122 9.52e-61 184
Msa1339230 AT4G27230.2 71.538 130 32 1 11 140 3 127 1.49e-59 181
Msa1339230 AT4G27230.1 71.538 130 32 1 11 140 3 127 1.49e-59 181
Msa1339230 AT3G20670.1 75.630 119 29 0 11 129 3 121 4.96e-59 179
Msa1339230 AT1G08880.1 71.654 127 33 1 7 130 2 128 3.61e-58 177
Msa1339230 AT5G54640.1 74.790 119 30 0 11 129 3 121 4.69e-58 177
Msa1339230 AT1G54690.1 72.868 129 29 3 3 130 5 128 1.24e-57 176
Msa1339230 AT2G38810.1 60.714 112 42 2 21 131 25 135 7.23e-37 123
Msa1339230 AT2G38810.4 60.714 112 42 2 21 131 25 135 7.23e-37 123
Msa1339230 AT2G38810.3 60.714 112 42 2 21 131 25 135 7.23e-37 123
Msa1339230 AT2G38810.2 60.714 112 42 2 21 131 25 135 7.23e-37 123
Msa1339230 AT1G52740.1 62.857 105 37 2 25 128 27 130 6.41e-35 118
Msa1339230 AT3G54560.1 60.185 108 41 2 25 131 29 135 1.29e-34 118
Msa1339230 AT3G54560.2 60.185 108 41 2 25 131 29 135 1.29e-34 118
Msa1339230 AT4G13570.1 48.913 92 45 2 32 122 28 118 2.04e-20 81.3
Msa1339230 AT4G13570.2 48.352 91 45 2 33 122 35 124 5.83e-20 80.1

Find 56 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.241789 tig0003042:-83313 None:intergenic
AATCAGATCTAAGCCTTCTT+TGG 0.242120 tig0003042:-83334 None:intergenic
GTCTTCTTAGGCAACAGAAT+TGG 0.279189 tig0003042:-83262 None:intergenic
TTAGCCCTTTCAGTCTTCTT+AGG 0.279305 tig0003042:-83274 None:intergenic
CGATTCTTCCGACTGGGAAT+TGG 0.285182 tig0003042:-82994 None:intergenic
CATTCCAAGGCATTTGTTGC+TGG 0.294047 tig0003042:+83159 Msa1339230:CDS
CTTGTCACCGACTTCTTCCT+TGG 0.303917 tig0003042:-82953 None:intergenic
GGCTCCCTTCTTTGTTGTCT+TGG 0.304409 tig0003042:-82909 None:intergenic
GAGCATATCGACCTTTCTTC+AGG 0.357439 tig0003042:-83024 None:intergenic
TGCTGCTGAGGTTCTTGAAT+TGG 0.361111 tig0003042:+83099 Msa1339230:CDS
GAGCTTGGGAAGTTGCTTGC+TGG 0.378028 tig0003042:+83199 Msa1339230:CDS
GGCATTTGTTGCTGGCTGTT+AGG 0.378218 tig0003042:+83167 Msa1339230:CDS
TCTTCTTAGGCAACAGAATT+GGG 0.398360 tig0003042:-83261 None:intergenic
GAAGGGAGCCGGTGGAAGAA+AGG 0.417242 tig0003042:+82922 Msa1339230:CDS
AACAGAATTGGGTTGATGTT+CGG 0.418504 tig0003042:-83250 None:intergenic
AAGGGAGCCGGTGGAAGAAA+GGG 0.429527 tig0003042:+82923 Msa1339230:CDS
CAATTCCCAGTCGGAAGAAT+CGG 0.431861 tig0003042:+82995 Msa1339230:CDS
AGAACTGCTGCTAGGTAAAC+CGG 0.439504 tig0003042:-83067 None:intergenic
ACCTCCTCCCTTTCTTCCAC+CGG 0.471747 tig0003042:-82930 None:intergenic
GCGTGTCGGCACCGGTGCTC+CGG 0.486643 tig0003042:+83048 Msa1339230:CDS
GAATTGGAGACCGGCTCTGA+TGG 0.486869 tig0003042:-82978 None:intergenic
TATGCTCAGCGTGTCGGCAC+CGG 0.489995 tig0003042:+83040 Msa1339230:CDS
TCTTGAATATCTTGCTGCTG+AGG 0.496391 tig0003042:+83087 Msa1339230:CDS
AAGCACCAAGACAACAAAGA+AGG 0.501816 tig0003042:+82904 Msa1339230:CDS
GTTAGGAATGATGAAGAGCT+TGG 0.506747 tig0003042:+83184 Msa1339230:CDS
AGCAAGGAACCAAAGAAGGC+TGG 0.510603 tig0003042:+83304 Msa1339230:CDS
GCTGAGGTTCTTGAATTGGC+AGG 0.519701 tig0003042:+83103 Msa1339230:CDS
CTCGTGATAACAAGAAGAAT+AGG 0.522113 tig0003042:+83134 Msa1339230:CDS
TTAGGAATGATGAAGAGCTT+GGG 0.524474 tig0003042:+83185 Msa1339230:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 tig0003042:+83321 Msa1339230:CDS
ACAGCCAGCAACAAATGCCT+TGG 0.526781 tig0003042:-83163 None:intergenic
TGCTAGGTAAACCGGAGCAC+CGG 0.537567 tig0003042:-83059 None:intergenic
ATCGGTCGATACCTGAAGAA+AGG 0.548682 tig0003042:+83013 Msa1339230:CDS
TATCGACCGATTCTTCCGAC+TGG 0.549955 tig0003042:-83001 None:intergenic
TGGTAGCAAGGAACCAAAGA+AGG 0.550474 tig0003042:+83300 Msa1339230:CDS
TGTTGCCTAAGAAGACTGAA+AGG 0.556495 tig0003042:+83269 Msa1339230:CDS
AGCACCAAGACAACAAAGAA+GGG 0.558080 tig0003042:+82905 Msa1339230:CDS
GAAGAAAGGGAGGAGGTCCA+AGG 0.558674 tig0003042:+82936 Msa1339230:CDS
TCCGACTGGGAATTGGAGAC+CGG 0.563841 tig0003042:-82987 None:intergenic
AAGAACACCACCATGAGCTA+TGG 0.573393 tig0003042:-83227 None:intergenic
AAGACAACAAAGAAGGGAGC+CGG 0.578427 tig0003042:+82911 Msa1339230:CDS
GATATTCAAGAACTGCTGCT+AGG 0.583613 tig0003042:-83075 None:intergenic
GCTGGTGTTACCATAGCTCA+TGG 0.586669 tig0003042:+83217 Msa1339230:CDS
GTGACAAGATCCATCAGAGC+CGG 0.589197 tig0003042:+82968 Msa1339230:CDS
GTTGCCTAAGAAGACTGAAA+GGG 0.589268 tig0003042:+83270 Msa1339230:CDS
GCCGGTGGAAGAAAGGGAGG+AGG 0.593863 tig0003042:+82929 Msa1339230:CDS
AAGGGCTAACACTGGTAGCA+AGG 0.594830 tig0003042:+83288 Msa1339230:CDS
ACAACAAAGAAGGGAGCCGG+TGG 0.595449 tig0003042:+82914 Msa1339230:CDS
GGAGCCGGTGGAAGAAAGGG+AGG 0.604037 tig0003042:+82926 Msa1339230:CDS
GGTCGATATGCTCAGCGTGT+CGG 0.611319 tig0003042:+83034 Msa1339230:CDS
ATCGACCGATTCTTCCGACT+GGG 0.613102 tig0003042:-83000 None:intergenic
AGGAGGTCCAAGGAAGAAGT+CGG 0.620460 tig0003042:+82946 Msa1339230:CDS
GCCGGTCTCCAATTCCCAGT+CGG 0.630576 tig0003042:+82986 Msa1339230:CDS
AGAAGAATAGGATCATTCCA+AGG 0.652341 tig0003042:+83146 Msa1339230:CDS
AAGACTGAAAGGGCTAACAC+TGG 0.665786 tig0003042:+83280 Msa1339230:CDS
GGTGTTACCATAGCTCATGG+TGG 0.742529 tig0003042:+83220 Msa1339230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTCGTGATAACAAGAAGAAT+AGG + tig0003042:83134-83153 Msa1339230:CDS 35.0%
AGAAGAATAGGATCATTCCA+AGG + tig0003042:83146-83165 Msa1339230:CDS 35.0%
TTAGGAATGATGAAGAGCTT+GGG + tig0003042:83185-83204 Msa1339230:CDS 35.0%
!! AACAGAATTGGGTTGATGTT+CGG - tig0003042:83253-83272 None:intergenic 35.0%
TCTTCTTAGGCAACAGAATT+GGG - tig0003042:83264-83283 None:intergenic 35.0%
AAGCACCAAGACAACAAAGA+AGG + tig0003042:82904-82923 Msa1339230:CDS 40.0%
AGCACCAAGACAACAAAGAA+GGG + tig0003042:82905-82924 Msa1339230:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG - tig0003042:83078-83097 None:intergenic 40.0%
TCTTGAATATCTTGCTGCTG+AGG + tig0003042:83087-83106 Msa1339230:CDS 40.0%
GTTAGGAATGATGAAGAGCT+TGG + tig0003042:83184-83203 Msa1339230:CDS 40.0%
GTCTTCTTAGGCAACAGAAT+TGG - tig0003042:83265-83284 None:intergenic 40.0%
TGTTGCCTAAGAAGACTGAA+AGG + tig0003042:83269-83288 Msa1339230:CDS 40.0%
GTTGCCTAAGAAGACTGAAA+GGG + tig0003042:83270-83289 Msa1339230:CDS 40.0%
TTAGCCCTTTCAGTCTTCTT+AGG - tig0003042:83277-83296 None:intergenic 40.0%
AAGACAACAAAGAAGGGAGC+CGG + tig0003042:82911-82930 Msa1339230:CDS 45.0%
CAATTCCCAGTCGGAAGAAT+CGG + tig0003042:82995-83014 Msa1339230:CDS 45.0%
ATCGGTCGATACCTGAAGAA+AGG + tig0003042:83013-83032 Msa1339230:CDS 45.0%
GAGCATATCGACCTTTCTTC+AGG - tig0003042:83027-83046 None:intergenic 45.0%
AGAACTGCTGCTAGGTAAAC+CGG - tig0003042:83070-83089 None:intergenic 45.0%
TGCTGCTGAGGTTCTTGAAT+TGG + tig0003042:83099-83118 Msa1339230:CDS 45.0%
! CATTCCAAGGCATTTGTTGC+TGG + tig0003042:83159-83178 Msa1339230:CDS 45.0%
AAGAACACCACCATGAGCTA+TGG - tig0003042:83230-83249 None:intergenic 45.0%
AAGACTGAAAGGGCTAACAC+TGG + tig0003042:83280-83299 Msa1339230:CDS 45.0%
TGGTAGCAAGGAACCAAAGA+AGG + tig0003042:83300-83319 Msa1339230:CDS 45.0%
! GGCTCCCTTCTTTGTTGTCT+TGG - tig0003042:82912-82931 None:intergenic 50.0%
AGGAGGTCCAAGGAAGAAGT+CGG + tig0003042:82946-82965 Msa1339230:CDS 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG - tig0003042:82956-82975 None:intergenic 50.0%
GTGACAAGATCCATCAGAGC+CGG + tig0003042:82968-82987 Msa1339230:CDS 50.0%
CGATTCTTCCGACTGGGAAT+TGG - tig0003042:82997-83016 None:intergenic 50.0%
! ATCGACCGATTCTTCCGACT+GGG - tig0003042:83003-83022 None:intergenic 50.0%
! TATCGACCGATTCTTCCGAC+TGG - tig0003042:83004-83023 None:intergenic 50.0%
GCTGAGGTTCTTGAATTGGC+AGG + tig0003042:83103-83122 Msa1339230:CDS 50.0%
ACAGCCAGCAACAAATGCCT+TGG - tig0003042:83166-83185 None:intergenic 50.0%
! GGCATTTGTTGCTGGCTGTT+AGG + tig0003042:83167-83186 Msa1339230:CDS 50.0%
!! GCTGGTGTTACCATAGCTCA+TGG + tig0003042:83217-83236 Msa1339230:CDS 50.0%
! GGTGTTACCATAGCTCATGG+TGG + tig0003042:83220-83239 Msa1339230:CDS 50.0%
AAGGGCTAACACTGGTAGCA+AGG + tig0003042:83288-83307 Msa1339230:CDS 50.0%
AGCAAGGAACCAAAGAAGGC+TGG + tig0003042:83304-83323 Msa1339230:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG - tig0003042:83316-83335 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG + tig0003042:83321-83340 Msa1339230:CDS 50.0%
ACAACAAAGAAGGGAGCCGG+TGG + tig0003042:82914-82933 Msa1339230:CDS 55.0%
AAGGGAGCCGGTGGAAGAAA+GGG + tig0003042:82923-82942 Msa1339230:CDS 55.0%
ACCTCCTCCCTTTCTTCCAC+CGG - tig0003042:82933-82952 None:intergenic 55.0%
GAAGAAAGGGAGGAGGTCCA+AGG + tig0003042:82936-82955 Msa1339230:CDS 55.0%
! GAATTGGAGACCGGCTCTGA+TGG - tig0003042:82981-83000 None:intergenic 55.0%
TCCGACTGGGAATTGGAGAC+CGG - tig0003042:82990-83009 None:intergenic 55.0%
GGTCGATATGCTCAGCGTGT+CGG + tig0003042:83034-83053 Msa1339230:CDS 55.0%
TGCTAGGTAAACCGGAGCAC+CGG - tig0003042:83062-83081 None:intergenic 55.0%
GAGCTTGGGAAGTTGCTTGC+TGG + tig0003042:83199-83218 Msa1339230:CDS 55.0%
GAAGGGAGCCGGTGGAAGAA+AGG + tig0003042:82922-82941 Msa1339230:CDS 60.0%
GCCGGTCTCCAATTCCCAGT+CGG + tig0003042:82986-83005 Msa1339230:CDS 60.0%
! TATGCTCAGCGTGTCGGCAC+CGG + tig0003042:83040-83059 Msa1339230:CDS 60.0%
GGAGCCGGTGGAAGAAAGGG+AGG + tig0003042:82926-82945 Msa1339230:CDS 65.0%
GCCGGTGGAAGAAAGGGAGG+AGG + tig0003042:82929-82948 Msa1339230:CDS 65.0%
!! GCGTGTCGGCACCGGTGCTC+CGG + tig0003042:83048-83067 Msa1339230:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0003042 gene 82896 83348 82896 ID=Msa1339230;Name=Msa1339230
tig0003042 mRNA 82896 83348 82896 ID=Msa1339230-mRNA-1;Parent=Msa1339230;Name=Msa1339230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|150
tig0003042 exon 82896 83348 82896 ID=Msa1339230-mRNA-1:exon:1979;Parent=Msa1339230-mRNA-1
tig0003042 CDS 82896 83348 82896 ID=Msa1339230-mRNA-1:cds;Parent=Msa1339230-mRNA-1
Gene Sequence

>Msa1339230

ATGGACGCAAGCACCAAGACAACAAAGAAGGGAGCCGGTGGAAGAAAGGGAGGAGGTCCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTCCAATTCCCAGTCGGAAGAATCGGTCGATACCTGAAGAAAGGTCGATATGCTCAGCGTGTCGGCACCGGTGCTCCGGTTTACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAATTGGCAGGAAACGCTGCTCGTGATAACAAGAAGAATAGGATCATTCCAAGGCATTTGTTGCTGGCTGTTAGGAATGATGAAGAGCTTGGGAAGTTGCTTGCTGGTGTTACCATAGCTCATGGTGGTGTTCTTCCGAACATCAACCCAATTCTGTTGCCTAAGAAGACTGAAAGGGCTAACACTGGTAGCAAGGAACCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>Msa1339230

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTERANTGSKEPKKAGKSPKKA*