AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320270 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320270 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0320270 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0320270 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0320270 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0320270 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0320270 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0320270 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0320270 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0320270 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0320270 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0320270 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0320270 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0320270 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0320270 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0320270 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320270 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320270 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320270 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320270 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320270 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320270 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320270 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0320270 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0320270 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0320270 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0320270 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0320270 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0320270 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0320270 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0320270 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0320270 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 38 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAAGCTCAGTGCTCTTC+TGG 0.304985 2_4:-20520963 None:intergenic
TCACGAAGTGCGACGGTTCC+TGG 0.318785 2_4:-20520928 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 2_4:+20521084 Msa0320270:CDS
GAAGCTTCCATTTCAAAGAT+TGG 0.365481 2_4:+20520990 Msa0320270:CDS
TCTCGAACCAATCTTTGAAA+TGG 0.396886 2_4:-20520997 None:intergenic
GGATTTGCGGGCTGTTTGTT+TGG 0.425629 2_4:-20520809 None:intergenic
CGGAAATCCGCTCCGGCGAC+AGG 0.432465 2_4:+20520877 Msa0320270:CDS
TTCTTCACTCCTCCTGTCGC+CGG 0.451049 2_4:-20520889 None:intergenic
GGATTTCCGAGCGGCCTTTG+TGG 0.451322 2_4:-20520863 None:intergenic
GAGCAGAAACAGCACTACTC+TGG 0.498444 2_4:-20521053 None:intergenic
AAGCCCCACCGTTTCCGTCC+AGG 0.502650 2_4:+20520910 Msa0320270:CDS
CGACGGTTCCTGGACGGAAA+CGG 0.511490 2_4:-20520918 None:intergenic
TTGTGCTATTCATGCTAAGA+GGG 0.513784 2_4:+20521128 Msa0320270:CDS
GGTTCCTGGACGGAAACGGT+GGG 0.514668 2_4:-20520914 None:intergenic
GGCCGCTCGGAAATCCGCTC+CGG 0.522574 2_4:+20520870 Msa0320270:CDS
CAAACAGCCCGCAAATCCAC+CGG 0.524480 2_4:+20520814 Msa0320270:CDS
AGAGCACTGAGCTTCTCATA+AGG 0.531497 2_4:+20520968 Msa0320270:CDS
CGGTTCCTGGACGGAAACGG+TGG 0.532129 2_4:-20520915 None:intergenic
AAACAGCACTACTCTGGAAA+CGG 0.532210 2_4:-20521047 None:intergenic
GAGGGTTACTATTATGCCTA+AGG 0.533362 2_4:+20521146 Msa0320270:CDS
CTCGCAAGCTGAATATCCTT+AGG 0.533935 2_4:-20521162 None:intergenic
TTTGTGCTATTCATGCTAAG+AGG 0.537531 2_4:+20521127 Msa0320270:CDS
AACTCGCCACAAAGGCCGCT+CGG 0.542091 2_4:+20520857 Msa0320270:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.559165 2_4:-20520954 None:intergenic
CACTCCTCCTGTCGCCGGAG+CGG 0.564263 2_4:-20520884 None:intergenic
GAGAAGAATCAGAGGCGAAA+GGG 0.568256 2_4:+20521182 Msa0320270:CDS
CGAGAAGAATCAGAGGCGAA+AGG 0.575054 2_4:+20521181 Msa0320270:CDS
AATCGCTCAGGATTTCAAAA+CGG 0.576806 2_4:+20521020 Msa0320270:CDS
GCGGATTTCACGAAGTGCGA+CGG 0.598942 2_4:-20520935 None:intergenic
GTTCCTGGACGGAAACGGTG+GGG 0.599384 2_4:-20520913 None:intergenic
AAATCCGCTCCGGCGACAGG+AGG 0.606452 2_4:+20520880 Msa0320270:CDS
ATTGGTTCGAGAAATCGCTC+AGG 0.615789 2_4:+20521008 Msa0320270:CDS
ACGCAAACAACTCGCCACAA+AGG 0.618065 2_4:+20520849 Msa0320270:CDS
GAAGTGCGACGGTTCCTGGA+CGG 0.655834 2_4:-20520924 None:intergenic
ACAGCCCGCAAATCCACCGG+CGG 0.662314 2_4:+20520817 Msa0320270:CDS
CAGCTTGCGAGAAGAATCAG+AGG 0.668283 2_4:+20521174 Msa0320270:CDS
TTTGTGGCGAGTTGTTTGCG+TGG 0.694492 2_4:-20520847 None:intergenic
CGCCGGAGCGGATTTCCGAG+CGG 0.708755 2_4:-20520872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAAGCTTCCATTTCAAAGAT+TGG + chr2_4:20520990-20521009 Msa0320270:CDS 35.0%
! TCTCGAACCAATCTTTGAAA+TGG - chr2_4:20521000-20521019 None:intergenic 35.0%
AATCGCTCAGGATTTCAAAA+CGG + chr2_4:20521020-20521039 Msa0320270:CDS 35.0%
! TTTGTGCTATTCATGCTAAG+AGG + chr2_4:20521127-20521146 Msa0320270:CDS 35.0%
! TTGTGCTATTCATGCTAAGA+GGG + chr2_4:20521128-20521147 Msa0320270:CDS 35.0%
! AAACAGCACTACTCTGGAAA+CGG - chr2_4:20521050-20521069 None:intergenic 40.0%
GAGGGTTACTATTATGCCTA+AGG + chr2_4:20521146-20521165 Msa0320270:CDS 40.0%
!! AGAGCACTGAGCTTCTCATA+AGG + chr2_4:20520968-20520987 Msa0320270:CDS 45.0%
!! ATTGGTTCGAGAAATCGCTC+AGG + chr2_4:20521008-20521027 Msa0320270:CDS 45.0%
GCTGCTGAAGCTTATCTTGT+TGG + chr2_4:20521084-20521103 Msa0320270:CDS 45.0%
CTCGCAAGCTGAATATCCTT+AGG - chr2_4:20521165-20521184 None:intergenic 45.0%
GAGAAGAATCAGAGGCGAAA+GGG + chr2_4:20521182-20521201 Msa0320270:CDS 45.0%
! GGATTTGCGGGCTGTTTGTT+TGG - chr2_4:20520812-20520831 None:intergenic 50.0%
TTTGTGGCGAGTTGTTTGCG+TGG - chr2_4:20520850-20520869 None:intergenic 50.0%
ACGCAAACAACTCGCCACAA+AGG + chr2_4:20520849-20520868 Msa0320270:CDS 50.0%
CAGTGCTCTTCTGGTACTTG+CGG - chr2_4:20520957-20520976 None:intergenic 50.0%
TGAGAAGCTCAGTGCTCTTC+TGG - chr2_4:20520966-20520985 None:intergenic 50.0%
GAGCAGAAACAGCACTACTC+TGG - chr2_4:20521056-20521075 None:intergenic 50.0%
CAGCTTGCGAGAAGAATCAG+AGG + chr2_4:20521174-20521193 Msa0320270:CDS 50.0%
CGAGAAGAATCAGAGGCGAA+AGG + chr2_4:20521181-20521200 Msa0320270:CDS 50.0%
CAAACAGCCCGCAAATCCAC+CGG + chr2_4:20520814-20520833 Msa0320270:CDS 55.0%
!! TTTTCCGCCGGTGGATTTGC+GGG - chr2_4:20520824-20520843 None:intergenic 55.0%
!! CTTTTCCGCCGGTGGATTTG+CGG - chr2_4:20520825-20520844 None:intergenic 55.0%
! TTTGCGTGGTGCTTTTCCGC+CGG - chr2_4:20520836-20520855 None:intergenic 55.0%
TTCTTCACTCCTCCTGTCGC+CGG - chr2_4:20520892-20520911 None:intergenic 55.0%
GCGGATTTCACGAAGTGCGA+CGG - chr2_4:20520938-20520957 None:intergenic 55.0%
AACTCGCCACAAAGGCCGCT+CGG + chr2_4:20520857-20520876 Msa0320270:CDS 60.0%
GGATTTCCGAGCGGCCTTTG+TGG - chr2_4:20520866-20520885 None:intergenic 60.0%
GTTCCTGGACGGAAACGGTG+GGG - chr2_4:20520916-20520935 None:intergenic 60.0%
GGTTCCTGGACGGAAACGGT+GGG - chr2_4:20520917-20520936 None:intergenic 60.0%
CGACGGTTCCTGGACGGAAA+CGG - chr2_4:20520921-20520940 None:intergenic 60.0%
GAAGTGCGACGGTTCCTGGA+CGG - chr2_4:20520927-20520946 None:intergenic 60.0%
TCACGAAGTGCGACGGTTCC+TGG - chr2_4:20520931-20520950 None:intergenic 60.0%
ACAGCCCGCAAATCCACCGG+CGG + chr2_4:20520817-20520836 Msa0320270:CDS 65.0%
AAATCCGCTCCGGCGACAGG+AGG + chr2_4:20520880-20520899 Msa0320270:CDS 65.0%
AAGCCCCACCGTTTCCGTCC+AGG + chr2_4:20520910-20520929 Msa0320270:CDS 65.0%
CGGTTCCTGGACGGAAACGG+TGG - chr2_4:20520918-20520937 None:intergenic 65.0%
! GCGTGGTGCTTTTCCGCCGG+TGG - chr2_4:20520833-20520852 None:intergenic 70.0%
GGCCGCTCGGAAATCCGCTC+CGG + chr2_4:20520870-20520889 Msa0320270:CDS 70.0%
! CGCCGGAGCGGATTTCCGAG+CGG - chr2_4:20520875-20520894 None:intergenic 70.0%
CGGAAATCCGCTCCGGCGAC+AGG + chr2_4:20520877-20520896 Msa0320270:CDS 70.0%
CACTCCTCCTGTCGCCGGAG+CGG - chr2_4:20520887-20520906 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 20520799 20521209 20520799 ID=Msa0320270;Name=Msa0320270
chr2_4 mRNA 20520799 20521209 20520799 ID=Msa0320270-mRNA-1;Parent=Msa0320270;Name=Msa0320270-mRNA-1;_AED=0.43;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|136
chr2_4 exon 20520799 20521209 20520799 ID=Msa0320270-mRNA-1:exon:7551;Parent=Msa0320270-mRNA-1
chr2_4 CDS 20520799 20521209 20520799 ID=Msa0320270-mRNA-1:cds;Parent=Msa0320270-mRNA-1
Gene Sequence

>Msa0320270

ATGGCACGTACCAAACAAACAGCCCGCAAATCCACCGGCGGAAAAGCACCACGCAAACAACTCGCCACAAAGGCCGCTCGGAAATCCGCTCCGGCGACAGGAGGAGTGAAGAAGCCCCACCGTTTCCGTCCAGGAACCGTCGCACTTCGTGAAATCCGCAAGTACCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCATTTCAAAGATTGGTTCGAGAAATCGCTCAGGATTTCAAAACGGATCTCCGTTTCCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGGGTTACTATTATGCCTAAGGATATTCAGCTTGCGAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>Msa0320270

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*