AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320290 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0320290 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0320290 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0320290 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0320290 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0320290 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0320290 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0320290 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0320290 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0320290 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0320290 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0320290 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0320290 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0320290 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0320290 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0320290 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320290 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320290 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320290 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320290 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320290 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0320290 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0320290 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0320290 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0320290 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0320290 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0320290 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0320290 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0320290 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0320290 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0320290 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0320290 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 38 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAAGCTCAGTGCTCTTC+TGG 0.304985 2_4:-20525165 None:intergenic
TCACGAAGTGCGACGGTTCC+TGG 0.318785 2_4:-20525130 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 2_4:+20525286 Msa0320290:CDS
GAAGCTTCCATTTCAAAGAT+TGG 0.365481 2_4:+20525192 Msa0320290:CDS
TCTCGAACCAATCTTTGAAA+TGG 0.396886 2_4:-20525199 None:intergenic
GGATTTGCGGGCTGTTTGTT+TGG 0.425629 2_4:-20525011 None:intergenic
CGGAAATCCGCTCCGGCGAC+AGG 0.432465 2_4:+20525079 Msa0320290:CDS
TTCTTCACTCCTCCTGTCGC+CGG 0.451049 2_4:-20525091 None:intergenic
GGATTTCCGAGCGGCCTTTG+TGG 0.451322 2_4:-20525065 None:intergenic
GAGCAGAAACAGCACTACTC+TGG 0.498444 2_4:-20525255 None:intergenic
AAGCCCCACCGTTTCCGTCC+AGG 0.502650 2_4:+20525112 Msa0320290:CDS
CGACGGTTCCTGGACGGAAA+CGG 0.511490 2_4:-20525120 None:intergenic
TTGTGCTATTCATGCTAAGA+GGG 0.513784 2_4:+20525330 Msa0320290:CDS
GGTTCCTGGACGGAAACGGT+GGG 0.514668 2_4:-20525116 None:intergenic
GGCCGCTCGGAAATCCGCTC+CGG 0.522574 2_4:+20525072 Msa0320290:CDS
CAAACAGCCCGCAAATCCAC+CGG 0.524480 2_4:+20525016 Msa0320290:CDS
AGAGCACTGAGCTTCTCATA+AGG 0.531497 2_4:+20525170 Msa0320290:CDS
CGGTTCCTGGACGGAAACGG+TGG 0.532129 2_4:-20525117 None:intergenic
AAACAGCACTACTCTGGAAA+CGG 0.532210 2_4:-20525249 None:intergenic
GAGGGTTACTATTATGCCTA+AGG 0.533362 2_4:+20525348 Msa0320290:CDS
CTCGCAAGCTGAATATCCTT+AGG 0.533935 2_4:-20525364 None:intergenic
TTTGTGCTATTCATGCTAAG+AGG 0.537531 2_4:+20525329 Msa0320290:CDS
AACTCGCCACAAAGGCCGCT+CGG 0.542091 2_4:+20525059 Msa0320290:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.559165 2_4:-20525156 None:intergenic
CACTCCTCCTGTCGCCGGAG+CGG 0.564263 2_4:-20525086 None:intergenic
GAGAAGAATCAGAGGCGAAA+GGG 0.568256 2_4:+20525384 Msa0320290:CDS
CGAGAAGAATCAGAGGCGAA+AGG 0.575054 2_4:+20525383 Msa0320290:CDS
AATCGCTCAGGATTTCAAAA+CGG 0.576806 2_4:+20525222 Msa0320290:CDS
GCGGATTTCACGAAGTGCGA+CGG 0.598942 2_4:-20525137 None:intergenic
GTTCCTGGACGGAAACGGTG+GGG 0.599384 2_4:-20525115 None:intergenic
AAATCCGCTCCGGCGACAGG+AGG 0.606452 2_4:+20525082 Msa0320290:CDS
ATTGGTTCGAGAAATCGCTC+AGG 0.615789 2_4:+20525210 Msa0320290:CDS
ACGCAAACAACTCGCCACAA+AGG 0.618065 2_4:+20525051 Msa0320290:CDS
GAAGTGCGACGGTTCCTGGA+CGG 0.655834 2_4:-20525126 None:intergenic
ACAGCCCGCAAATCCACCGG+CGG 0.662314 2_4:+20525019 Msa0320290:CDS
CAGCTTGCGAGAAGAATCAG+AGG 0.668283 2_4:+20525376 Msa0320290:CDS
TTTGTGGCGAGTTGTTTGCG+TGG 0.694492 2_4:-20525049 None:intergenic
CGCCGGAGCGGATTTCCGAG+CGG 0.708755 2_4:-20525074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAAGCTTCCATTTCAAAGAT+TGG + chr2_4:20525192-20525211 Msa0320290:CDS 35.0%
! TCTCGAACCAATCTTTGAAA+TGG - chr2_4:20525202-20525221 None:intergenic 35.0%
AATCGCTCAGGATTTCAAAA+CGG + chr2_4:20525222-20525241 Msa0320290:CDS 35.0%
! TTTGTGCTATTCATGCTAAG+AGG + chr2_4:20525329-20525348 Msa0320290:CDS 35.0%
! TTGTGCTATTCATGCTAAGA+GGG + chr2_4:20525330-20525349 Msa0320290:CDS 35.0%
! AAACAGCACTACTCTGGAAA+CGG - chr2_4:20525252-20525271 None:intergenic 40.0%
GAGGGTTACTATTATGCCTA+AGG + chr2_4:20525348-20525367 Msa0320290:CDS 40.0%
!! AGAGCACTGAGCTTCTCATA+AGG + chr2_4:20525170-20525189 Msa0320290:CDS 45.0%
!! ATTGGTTCGAGAAATCGCTC+AGG + chr2_4:20525210-20525229 Msa0320290:CDS 45.0%
GCTGCTGAAGCTTATCTTGT+TGG + chr2_4:20525286-20525305 Msa0320290:CDS 45.0%
CTCGCAAGCTGAATATCCTT+AGG - chr2_4:20525367-20525386 None:intergenic 45.0%
GAGAAGAATCAGAGGCGAAA+GGG + chr2_4:20525384-20525403 Msa0320290:CDS 45.0%
! GGATTTGCGGGCTGTTTGTT+TGG - chr2_4:20525014-20525033 None:intergenic 50.0%
TTTGTGGCGAGTTGTTTGCG+TGG - chr2_4:20525052-20525071 None:intergenic 50.0%
ACGCAAACAACTCGCCACAA+AGG + chr2_4:20525051-20525070 Msa0320290:CDS 50.0%
CAGTGCTCTTCTGGTACTTG+CGG - chr2_4:20525159-20525178 None:intergenic 50.0%
TGAGAAGCTCAGTGCTCTTC+TGG - chr2_4:20525168-20525187 None:intergenic 50.0%
GAGCAGAAACAGCACTACTC+TGG - chr2_4:20525258-20525277 None:intergenic 50.0%
CAGCTTGCGAGAAGAATCAG+AGG + chr2_4:20525376-20525395 Msa0320290:CDS 50.0%
CGAGAAGAATCAGAGGCGAA+AGG + chr2_4:20525383-20525402 Msa0320290:CDS 50.0%
CAAACAGCCCGCAAATCCAC+CGG + chr2_4:20525016-20525035 Msa0320290:CDS 55.0%
!! TTTTCCGCCGGTGGATTTGC+GGG - chr2_4:20525026-20525045 None:intergenic 55.0%
!! CTTTTCCGCCGGTGGATTTG+CGG - chr2_4:20525027-20525046 None:intergenic 55.0%
! TTTGCGTGGTGCTTTTCCGC+CGG - chr2_4:20525038-20525057 None:intergenic 55.0%
TTCTTCACTCCTCCTGTCGC+CGG - chr2_4:20525094-20525113 None:intergenic 55.0%
GCGGATTTCACGAAGTGCGA+CGG - chr2_4:20525140-20525159 None:intergenic 55.0%
AACTCGCCACAAAGGCCGCT+CGG + chr2_4:20525059-20525078 Msa0320290:CDS 60.0%
GGATTTCCGAGCGGCCTTTG+TGG - chr2_4:20525068-20525087 None:intergenic 60.0%
GTTCCTGGACGGAAACGGTG+GGG - chr2_4:20525118-20525137 None:intergenic 60.0%
GGTTCCTGGACGGAAACGGT+GGG - chr2_4:20525119-20525138 None:intergenic 60.0%
CGACGGTTCCTGGACGGAAA+CGG - chr2_4:20525123-20525142 None:intergenic 60.0%
GAAGTGCGACGGTTCCTGGA+CGG - chr2_4:20525129-20525148 None:intergenic 60.0%
TCACGAAGTGCGACGGTTCC+TGG - chr2_4:20525133-20525152 None:intergenic 60.0%
ACAGCCCGCAAATCCACCGG+CGG + chr2_4:20525019-20525038 Msa0320290:CDS 65.0%
AAATCCGCTCCGGCGACAGG+AGG + chr2_4:20525082-20525101 Msa0320290:CDS 65.0%
AAGCCCCACCGTTTCCGTCC+AGG + chr2_4:20525112-20525131 Msa0320290:CDS 65.0%
CGGTTCCTGGACGGAAACGG+TGG - chr2_4:20525120-20525139 None:intergenic 65.0%
! GCGTGGTGCTTTTCCGCCGG+TGG - chr2_4:20525035-20525054 None:intergenic 70.0%
GGCCGCTCGGAAATCCGCTC+CGG + chr2_4:20525072-20525091 Msa0320290:CDS 70.0%
! CGCCGGAGCGGATTTCCGAG+CGG - chr2_4:20525077-20525096 None:intergenic 70.0%
CGGAAATCCGCTCCGGCGAC+AGG + chr2_4:20525079-20525098 Msa0320290:CDS 70.0%
CACTCCTCCTGTCGCCGGAG+CGG - chr2_4:20525089-20525108 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 20525001 20525411 20525001 ID=Msa0320290;Name=Msa0320290
chr2_4 mRNA 20525001 20525411 20525001 ID=Msa0320290-mRNA-1;Parent=Msa0320290;Name=Msa0320290-mRNA-1;_AED=0.43;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|136
chr2_4 exon 20525001 20525411 20525001 ID=Msa0320290-mRNA-1:exon:7552;Parent=Msa0320290-mRNA-1
chr2_4 CDS 20525001 20525411 20525001 ID=Msa0320290-mRNA-1:cds;Parent=Msa0320290-mRNA-1
Gene Sequence

>Msa0320290

ATGGCACGTACCAAACAAACAGCCCGCAAATCCACCGGCGGAAAAGCACCACGCAAACAACTCGCCACAAAGGCCGCTCGGAAATCCGCTCCGGCGACAGGAGGAGTGAAGAAGCCCCACCGTTTCCGTCCAGGAACCGTCGCACTTCGTGAAATCCGCAAGTACCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCATTTCAAAGATTGGTTCGAGAAATCGCTCAGGATTTCAAAACGGATCTCCGTTTCCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGGGTTACTATTATGCCTAAGGATATTCAGCTTGCGAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>Msa0320290

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*