AlfalfaGEDB | Msa0320250

M. sativa cultivar zhongmu-4 / Msa0320250

KEGG: K11253
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320250	KYP58465.1	100.000	136	0	0	1	136	5	140	1.76e-92	273

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320250	sp\|P68428\|H32_WHEAT	100.000	136	0	0	1	136	1	136	5.15e-95	272

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030070	Msa0320250	0.872380	3.326365e-67	-8.615850e-47
Msa0030690	Msa0320250	0.804135	2.498494e-49	-8.615850e-47
Msa0032230	Msa0320250	0.802468	5.531367e-49	-8.615850e-47
Msa0050530	Msa0320250	0.811745	6.004429e-51	-8.615850e-47
Msa0067380	Msa0320250	0.807294	5.423622e-50	-8.615850e-47
Msa0090440	Msa0320250	0.817274	3.589434e-52	-8.615850e-47
Msa0091350	Msa0320250	0.823545	1.307735e-53	-8.615850e-47
Msa0100600	Msa0320250	0.813160	2.945225e-51	-8.615850e-47
Msa0100890	Msa0320250	0.801707	7.928837e-49	-8.615850e-47
Msa0113410	Msa0320250	0.818630	1.773212e-52	-8.615850e-47
Msa0125590	Msa0320250	0.864132	1.514608e-64	-8.615850e-47
Msa0127270	Msa0320250	0.807966	3.904914e-50	-8.615850e-47
Msa0127340	Msa0320250	0.829323	5.490473e-55	-8.615850e-47
Msa0158900	Msa0320250	0.804793	1.821780e-49	-8.615850e-47
Msa0165830	Msa0320250	0.801567	8.472487e-49	-8.615850e-47
Msa0170070	Msa0320250	0.857287	1.808703e-62	-8.615850e-47
Msa0186180	Msa0320250	0.846603	1.958047e-59	-8.615850e-47
Msa0204970	Msa0320250	0.839599	1.438795e-57	-8.615850e-47
Msa0225800	Msa0320250	0.801560	8.501950e-49	-8.615850e-47
Msa0249870	Msa0320250	0.834648	2.655058e-56	-8.615850e-47
Msa0260630	Msa0320250	0.805832	1.103408e-49	-8.615850e-47
Msa0281540	Msa0320250	0.822453	2.350115e-53	-8.615850e-47
Msa0320250	Msa0320270	0.999351	3.917050e-305	-8.615850e-47
Msa0320250	Msa0320290	0.996830	7.753177e-233	-8.615850e-47
Msa0320250	Msa0320580	0.835030	2.126988e-56	-8.615850e-47
Msa0320250	Msa0344110	0.836498	9.033556e-57	-8.615850e-47
Msa0320250	Msa0364680	0.804359	2.244154e-49	-8.615850e-47
Msa0320250	Msa0373170	0.847886	8.704571e-60	-8.615850e-47
Msa0320250	Msa0382230	0.817264	3.608081e-52	-8.615850e-47
Msa0320250	Msa0383940	0.804866	1.758554e-49	-8.615850e-47
Msa0320250	Msa0412130	0.834005	3.848206e-56	-8.615850e-47
Msa0320250	Msa0415820	0.833037	6.711632e-56	-8.615850e-47
Msa0320250	Msa0416210	0.803203	3.898912e-49	-8.615850e-47
Msa0320250	Msa0420670	0.808474	3.042743e-50	-8.615850e-47
Msa0320250	Msa0421130	0.802944	4.410697e-49	-8.615850e-47
Msa0320250	Msa0421750	0.834851	2.359658e-56	-8.615850e-47
Msa0320250	Msa0433760	0.846971	1.553315e-59	-8.615850e-47
Msa0320250	Msa0434360	0.820170	7.902551e-53	-8.615850e-47
Msa0320250	Msa0456670	0.833079	6.552854e-56	-8.615850e-47
Msa0320250	Msa0461550	0.812475	4.161136e-51	-8.615850e-47
Msa0320250	Msa0466520	0.812956	3.265554e-51	-8.615850e-47
Msa0320250	Msa0467690	0.844634	6.694065e-59	-8.615850e-47
Msa0320250	Msa0477630	0.833646	4.733326e-56	-8.615850e-47
Msa0320250	Msa0478140	0.831406	1.700210e-55	-8.615850e-47
Msa0320250	Msa0478200	0.827870	1.231814e-54	-8.615850e-47
Msa0320250	Msa0503990	0.849252	3.643978e-60	-8.615850e-47
Msa0320250	Msa0506540	0.819701	1.011286e-52	-8.615850e-47
Msa0320250	Msa0506880	0.843650	1.229890e-58	-8.615850e-47
Msa0320250	Msa0507020	0.807661	4.531734e-50	-8.615850e-47
Msa0320250	Msa0507810	0.852202	5.387975e-61	-8.615850e-47
Msa0320250	Msa0507820	0.823177	1.593929e-53	-8.615850e-47
Msa0320250	Msa0507920	0.850640	1.489647e-60	-8.615850e-47
Msa0320250	Msa0512530	0.809275	2.050625e-50	-8.615850e-47
Msa0320250	Msa0513080	0.801803	7.579523e-49	-8.615850e-47
Msa0320250	Msa0514020	0.840881	6.655378e-58	-8.615850e-47
Msa0320250	Msa0520680	0.818029	2.426020e-52	-8.615850e-47
Msa0320250	Msa0521680	0.831699	1.439411e-55	-8.615850e-47
Msa0320250	Msa0523380	0.800056	1.723850e-48	-8.615850e-47
Msa0320250	Msa0527850	0.835921	1.266483e-56	-8.615850e-47
Msa0320250	Msa0541740	0.832118	1.134184e-55	-8.615850e-47
Msa0320250	Msa0544410	0.848852	4.705726e-60	-8.615850e-47
Msa0320250	Msa0548000	0.864095	1.556232e-64	-8.615850e-47
Msa0320250	Msa0550590	0.813292	2.755090e-51	-8.615850e-47
Msa0320250	Msa0554300	0.842510	2.473959e-58	-8.615850e-47
Msa0320250	Msa0561560	0.814926	1.201402e-51	-8.615850e-47
Msa0320250	Msa0572830	0.824834	6.513140e-54	-8.615850e-47
Msa0320250	Msa0575710	0.804664	1.937646e-49	-8.615850e-47
Msa0320250	Msa0582460	0.831619	1.506657e-55	-8.615850e-47
Msa0320250	Msa0584530	0.821344	4.244897e-53	-8.615850e-47
Msa0320250	Msa0586200	0.839016	2.039229e-57	-8.615850e-47
Msa0320250	Msa0603410	0.827832	1.258037e-54	-8.615850e-47
Msa0320250	Msa0608350	0.804020	2.639508e-49	-8.615850e-47
Msa0320250	Msa0612410	0.818451	1.947123e-52	-8.615850e-47
Msa0320250	Msa0620500	0.820470	6.743780e-53	-8.615850e-47
Msa0320250	Msa0625620	0.836194	1.079387e-56	-8.615850e-47
Msa0320250	Msa0640800	0.858129	1.018517e-62	-8.615850e-47
Msa0320250	Msa0645340	0.831239	1.868265e-55	-8.615850e-47
Msa0320250	Msa0647440	0.824697	7.015690e-54	-8.615850e-47
Msa0320250	Msa0648760	0.845131	4.916529e-59	-8.615850e-47
Msa0320250	Msa0653160	0.819349	1.217234e-52	-8.615850e-47
Msa0320250	Msa0657210	0.809875	1.524541e-50	-8.615850e-47
Msa0320250	Msa0659200	0.860295	2.281721e-63	-8.615850e-47
Msa0320250	Msa0664070	0.805558	1.259804e-49	-8.615850e-47
Msa0320250	Msa0684750	0.802464	5.540185e-49	-8.615850e-47
Msa0320250	Msa0688620	0.843273	1.550792e-58	-8.615850e-47
Msa0320250	Msa0690320	0.834439	2.996285e-56	-8.615850e-47
Msa0320250	Msa0691270	0.813988	1.937213e-51	-8.615850e-47
Msa0320250	Msa0701250	0.829363	5.368364e-55	-8.615850e-47
Msa0320250	Msa0705180	0.862330	5.471272e-64	-8.615850e-47
Msa0320250	Msa0717900	0.832619	8.526872e-56	-8.615850e-47
Msa0320250	Msa0718040	0.813589	2.371118e-51	-8.615850e-47
Msa0320250	Msa0720280	0.878397	2.902258e-69	-8.615850e-47
Msa0320250	Msa0737130	0.805350	1.392746e-49	-8.615850e-47
Msa0320250	Msa0737140	0.829822	4.151546e-55	-8.615850e-47
Msa0320250	Msa0749190	0.838129	3.455098e-57	-8.615850e-47
Msa0320250	Msa0750520	0.845753	3.336080e-59	-8.615850e-47
Msa0320250	Msa0778600	0.803653	3.145598e-49	-8.615850e-47
Msa0320250	Msa0803140	0.881241	2.826228e-70	-8.615850e-47
Msa0320250	Msa0816080	0.824111	9.633470e-54	-8.615850e-47
Msa0320250	Msa0816230	0.817678	2.910794e-52	-8.615850e-47
Msa0320250	Msa0837680	0.805639	1.211402e-49	-8.615850e-47
Msa0320250	Msa0841500	0.894233	2.990486e-75	-8.615850e-47
Msa0320250	Msa0856610	0.820003	8.629681e-53	-8.615850e-47
Msa0320250	Msa0900090	0.817997	2.466714e-52	-8.615850e-47
Msa0320250	Msa0909760	0.833380	5.514487e-56	-8.615850e-47
Msa0320250	Msa0929900	0.803789	2.947811e-49	-8.615850e-47
Msa0320250	Msa0965470	0.813102	3.033975e-51	-8.615850e-47
Msa0320250	Msa1000780	0.803762	2.986889e-49	-8.615850e-47
Msa0320250	Msa1008670	0.844177	8.882702e-59	-8.615850e-47
Msa0320250	Msa1018960	0.851651	7.724132e-61	-8.615850e-47
Msa0320250	Msa1033920	0.820699	5.975245e-53	-8.615850e-47
Msa0320250	Msa1039380	0.828341	9.487575e-55	-8.615850e-47
Msa0320250	Msa1060900	0.808217	3.452563e-50	-8.615850e-47
Msa0320250	Msa1071410	0.844080	9.431725e-59	-8.615850e-47
Msa0320250	Msa1078860	0.807842	4.148495e-50	-8.615850e-47
Msa0320250	Msa1078990	0.807656	4.543666e-50	-8.615850e-47
Msa0320250	Msa1082380	0.816491	5.381285e-52	-8.615850e-47
Msa0320250	Msa1106220	0.806038	9.988514e-50	-8.615850e-47
Msa0320250	Msa1126620	0.809102	2.233333e-50	-8.615850e-47
Msa0320250	Msa1144990	0.831346	1.758909e-55	-8.615850e-47
Msa0320250	Msa1147560	0.865362	6.241797e-65	-8.615850e-47
Msa0320250	Msa1162880	0.847091	1.440155e-59	-8.615850e-47
Msa0320250	Msa1165480	0.800377	1.482975e-48	-8.615850e-47
Msa0320250	Msa1169170	0.814898	1.218628e-51	-8.615850e-47
Msa0320250	Msa1169690	0.802066	6.691940e-49	-8.615850e-47
Msa0320250	Msa1191000	0.831425	1.681596e-55	-8.615850e-47
Msa0320250	Msa1215980	0.821564	3.776841e-53	-8.615850e-47
Msa0320250	Msa1231780	0.818079	2.363366e-52	-8.615850e-47
Msa0320250	Msa1254420	0.809111	2.223386e-50	-8.615850e-47
Msa0320250	Msa1267930	0.829234	5.768511e-55	-8.615850e-47
Msa0320250	Msa1294790	0.810234	1.275613e-50	-8.615850e-47
Msa0320250	Msa1339230	0.888893	3.940857e-73	-8.615850e-47
Msa0320250	Msa1361930	0.821594	3.715321e-53	-8.615850e-47
Msa0320250	Msa1381470	0.825115	5.589620e-54	-8.615850e-47
Msa0320250	Msa1381800	0.843748	1.157937e-58	-8.615850e-47
Msa0320250	Msa1387500	0.844251	8.489204e-59	-8.615850e-47
Msa0320250	Msa1390210	0.816072	6.677473e-52	-8.615850e-47
Msa0320250	Msa1393560	0.805676	1.190156e-49	-8.615850e-47
Msa0320250	Msa1406720	0.818505	1.892754e-52	-8.615850e-47
Msa0320250	Msa1410190	0.910377	2.005383e-82	-8.615850e-47
Msa0320250	Msa1424370	0.874419	6.856805e-68	-8.615850e-47
Msa0320250	Msa1434640	0.807471	4.973129e-50	-8.615850e-47
Msa0320250	Msa1435100	0.803043	4.208075e-49	-8.615850e-47
Msa0320250	Msa1439730	0.812220	4.730759e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320250	MtrunA17_Chr5g0410931	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr5g0410951	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr8g0383851	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr8g0383781	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr8g0390361	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr2g0295191	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr7g0237661	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr4g0033041	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr7g0218971	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr4g0046301	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr8g0364801	100.000	136	0	0	1	136	1	136	3.79e-96	272
Msa0320250	MtrunA17_Chr4g0054161	97.059	136	4	0	1	136	1	136	2.60e-93	265
Msa0320250	MtrunA17_Chr8g0363741	97.059	136	4	0	1	136	1	136	2.60e-93	265
Msa0320250	MtrunA17_Chr1g0156021	97.059	136	4	0	1	136	1	136	2.60e-93	265
Msa0320250	MtrunA17_Chr4g0054151	97.059	136	4	0	1	136	4	139	2.61e-93	265
Msa0320250	MtrunA17_Chr8g0388121	96.324	136	5	0	1	136	1	136	1.09e-92	264
Msa0320250	MtrunA17_Chr2g0319141	89.706	136	14	0	1	136	1	136	1.10e-86	248
Msa0320250	MtrunA17_Chr2g0319151	89.706	136	14	0	1	136	1	136	1.10e-86	248
Msa0320250	MtrunA17_Chr2g0319131	85.616	146	11	1	1	136	1	146	1.38e-82	238
Msa0320250	MtrunA17_Chr2g0319121	84.559	136	19	2	1	135	1	135	5.82e-75	219
Msa0320250	MtrunA17_Chr7g0218961	86.607	112	15	0	25	136	2	113	8.01e-66	195
Msa0320250	MtrunA17_Chr7g0222991	67.164	134	43	1	1	134	10	142	5.47e-56	171
Msa0320250	MtrunA17_Chr5g0407311	67.241	116	35	2	1	116	1	113	1.29e-45	144
Msa0320250	MtrunA17_Chr7g0223001	58.647	133	41	3	3	134	27	146	5.46e-43	139
Msa0320250	MtrunA17_Chr7g0240791	100.000	66	0	0	1	66	1	66	9.61e-41	133
Msa0320250	MtrunA17_Chr8g0347231	55.102	98	43	1	37	133	28	125	3.80e-30	105

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0320250	AT1G09200.1	99.265	136	1	0	1	136	1	136	1.62e-95	271
Msa0320250	AT5G65360.1	99.265	136	1	0	1	136	1	136	1.62e-95	271
Msa0320250	AT5G10390.1	99.265	136	1	0	1	136	1	136	1.62e-95	271
Msa0320250	AT5G10400.1	99.265	136	1	0	1	136	1	136	1.62e-95	271
Msa0320250	AT3G27360.1	99.265	136	1	0	1	136	1	136	1.62e-95	271
Msa0320250	AT4G40030.3	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT4G40030.1	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT4G40030.4	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT5G10980.1	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT4G40040.2	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT4G40040.3	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT4G40040.1	97.059	136	4	0	1	136	1	136	3.59e-93	265
Msa0320250	AT4G40030.2	97.059	136	4	0	1	136	29	164	7.05e-93	266
Msa0320250	AT5G65350.1	94.853	136	7	0	1	136	1	136	5.30e-91	260
Msa0320250	AT1G75600.1	93.382	136	9	0	1	136	1	136	1.25e-89	256
Msa0320250	AT1G13370.1	93.382	136	9	0	1	136	1	136	5.55e-89	254
Msa0320250	AT1G19890.1	89.781	137	13	1	1	136	1	137	1.07e-83	241
Msa0320250	AT5G12910.1	71.111	135	34	2	1	135	1	130	9.90e-67	198
Msa0320250	AT1G01370.2	53.982	113	49	2	24	134	63	174	2.97e-31	110
Msa0320250	AT1G01370.1	53.982	113	49	2	24	134	63	174	2.97e-31	110
Msa0320250	AT1G01370.4	53.982	113	49	2	24	134	63	174	2.97e-31	110
Msa0320250	AT1G01370.3	53.982	113	49	2	24	134	63	174	2.97e-31	110

Find 38 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGAAGCTCAGTGCTCTTC+TGG	0.304985	2_4:-20516758	None:intergenic
TCACGAAGTGCGACGGTTCC+TGG	0.318785	2_4:-20516723	None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG	0.357332	2_4:+20516879	Msa0320250:CDS
GAAGCTTCCATTTCAAAGAT+TGG	0.365481	2_4:+20516785	Msa0320250:CDS
TCTCGAACCAATCTTTGAAA+TGG	0.396886	2_4:-20516792	None:intergenic
GGATTTGCGGGCTGTTTGTT+TGG	0.425629	2_4:-20516604	None:intergenic
CGGAAATCCGCTCCGGCGAC+AGG	0.432465	2_4:+20516672	Msa0320250:CDS
TTCTTCACTCCTCCTGTCGC+CGG	0.451049	2_4:-20516684	None:intergenic
GGATTTCCGAGCGGCCTTTG+TGG	0.451322	2_4:-20516658	None:intergenic
GAGCAGAAACAGCACTACTC+TGG	0.498444	2_4:-20516848	None:intergenic
AAGCCCCACCGTTTCCGTCC+AGG	0.502650	2_4:+20516705	Msa0320250:CDS
CGACGGTTCCTGGACGGAAA+CGG	0.511490	2_4:-20516713	None:intergenic
TTGTGCTATTCATGCTAAGA+GGG	0.513784	2_4:+20516923	Msa0320250:CDS
GGTTCCTGGACGGAAACGGT+GGG	0.514668	2_4:-20516709	None:intergenic
GGCCGCTCGGAAATCCGCTC+CGG	0.522574	2_4:+20516665	Msa0320250:CDS
CAAACAGCCCGCAAATCCAC+CGG	0.524480	2_4:+20516609	Msa0320250:CDS
AGAGCACTGAGCTTCTCATA+AGG	0.531497	2_4:+20516763	Msa0320250:CDS
CGGTTCCTGGACGGAAACGG+TGG	0.532129	2_4:-20516710	None:intergenic
AAACAGCACTACTCTGGAAA+CGG	0.532210	2_4:-20516842	None:intergenic
GAGGGTTACTATTATGCCTA+AGG	0.533362	2_4:+20516941	Msa0320250:CDS
CTCGCAAGCTGAATATCCTT+AGG	0.533935	2_4:-20516957	None:intergenic
TTTGTGCTATTCATGCTAAG+AGG	0.537531	2_4:+20516922	Msa0320250:CDS
AACTCGCCACAAAGGCCGCT+CGG	0.542091	2_4:+20516652	Msa0320250:CDS
CAGTGCTCTTCTGGTACTTG+CGG	0.559165	2_4:-20516749	None:intergenic
CACTCCTCCTGTCGCCGGAG+CGG	0.564263	2_4:-20516679	None:intergenic
GAGAAGAATCAGAGGCGAAA+GGG	0.568256	2_4:+20516977	Msa0320250:CDS
CGAGAAGAATCAGAGGCGAA+AGG	0.575054	2_4:+20516976	Msa0320250:CDS
AATCGCTCAGGATTTCAAAA+CGG	0.576806	2_4:+20516815	Msa0320250:CDS
GCGGATTTCACGAAGTGCGA+CGG	0.598942	2_4:-20516730	None:intergenic
GTTCCTGGACGGAAACGGTG+GGG	0.599384	2_4:-20516708	None:intergenic
AAATCCGCTCCGGCGACAGG+AGG	0.606452	2_4:+20516675	Msa0320250:CDS
ATTGGTTCGAGAAATCGCTC+AGG	0.615789	2_4:+20516803	Msa0320250:CDS
ACGCAAACAACTCGCCACAA+AGG	0.618065	2_4:+20516644	Msa0320250:CDS
GAAGTGCGACGGTTCCTGGA+CGG	0.655834	2_4:-20516719	None:intergenic
ACAGCCCGCAAATCCACCGG+CGG	0.662314	2_4:+20516612	Msa0320250:CDS
CAGCTTGCGAGAAGAATCAG+AGG	0.668283	2_4:+20516969	Msa0320250:CDS
TTTGTGGCGAGTTGTTTGCG+TGG	0.694492	2_4:-20516642	None:intergenic
CGCCGGAGCGGATTTCCGAG+CGG	0.708755	2_4:-20516667	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAGCTTCCATTTCAAAGAT+TGG	+	chr2_4:20516785-20516804	Msa0320250:CDS	35.0%
!	TCTCGAACCAATCTTTGAAA+TGG	-	chr2_4:20516795-20516814	None:intergenic	35.0%
	AATCGCTCAGGATTTCAAAA+CGG	+	chr2_4:20516815-20516834	Msa0320250:CDS	35.0%
!	TTTGTGCTATTCATGCTAAG+AGG	+	chr2_4:20516922-20516941	Msa0320250:CDS	35.0%
!	TTGTGCTATTCATGCTAAGA+GGG	+	chr2_4:20516923-20516942	Msa0320250:CDS	35.0%
!	AAACAGCACTACTCTGGAAA+CGG	-	chr2_4:20516845-20516864	None:intergenic	40.0%
	GAGGGTTACTATTATGCCTA+AGG	+	chr2_4:20516941-20516960	Msa0320250:CDS	40.0%
!!	AGAGCACTGAGCTTCTCATA+AGG	+	chr2_4:20516763-20516782	Msa0320250:CDS	45.0%
!!	ATTGGTTCGAGAAATCGCTC+AGG	+	chr2_4:20516803-20516822	Msa0320250:CDS	45.0%
	GCTGCTGAAGCTTATCTTGT+TGG	+	chr2_4:20516879-20516898	Msa0320250:CDS	45.0%
	CTCGCAAGCTGAATATCCTT+AGG	-	chr2_4:20516960-20516979	None:intergenic	45.0%
	GAGAAGAATCAGAGGCGAAA+GGG	+	chr2_4:20516977-20516996	Msa0320250:CDS	45.0%
!	GGATTTGCGGGCTGTTTGTT+TGG	-	chr2_4:20516607-20516626	None:intergenic	50.0%
	TTTGTGGCGAGTTGTTTGCG+TGG	-	chr2_4:20516645-20516664	None:intergenic	50.0%
	ACGCAAACAACTCGCCACAA+AGG	+	chr2_4:20516644-20516663	Msa0320250:CDS	50.0%
	CAGTGCTCTTCTGGTACTTG+CGG	-	chr2_4:20516752-20516771	None:intergenic	50.0%
	TGAGAAGCTCAGTGCTCTTC+TGG	-	chr2_4:20516761-20516780	None:intergenic	50.0%
	GAGCAGAAACAGCACTACTC+TGG	-	chr2_4:20516851-20516870	None:intergenic	50.0%
	CAGCTTGCGAGAAGAATCAG+AGG	+	chr2_4:20516969-20516988	Msa0320250:CDS	50.0%
	CGAGAAGAATCAGAGGCGAA+AGG	+	chr2_4:20516976-20516995	Msa0320250:CDS	50.0%
	CAAACAGCCCGCAAATCCAC+CGG	+	chr2_4:20516609-20516628	Msa0320250:CDS	55.0%
!!	TTTTCCGCCGGTGGATTTGC+GGG	-	chr2_4:20516619-20516638	None:intergenic	55.0%
!!	CTTTTCCGCCGGTGGATTTG+CGG	-	chr2_4:20516620-20516639	None:intergenic	55.0%
!	TTTGCGTGGTGCTTTTCCGC+CGG	-	chr2_4:20516631-20516650	None:intergenic	55.0%
	TTCTTCACTCCTCCTGTCGC+CGG	-	chr2_4:20516687-20516706	None:intergenic	55.0%
	GCGGATTTCACGAAGTGCGA+CGG	-	chr2_4:20516733-20516752	None:intergenic	55.0%
	AACTCGCCACAAAGGCCGCT+CGG	+	chr2_4:20516652-20516671	Msa0320250:CDS	60.0%
	GGATTTCCGAGCGGCCTTTG+TGG	-	chr2_4:20516661-20516680	None:intergenic	60.0%
	GTTCCTGGACGGAAACGGTG+GGG	-	chr2_4:20516711-20516730	None:intergenic	60.0%
	GGTTCCTGGACGGAAACGGT+GGG	-	chr2_4:20516712-20516731	None:intergenic	60.0%
	CGACGGTTCCTGGACGGAAA+CGG	-	chr2_4:20516716-20516735	None:intergenic	60.0%
	GAAGTGCGACGGTTCCTGGA+CGG	-	chr2_4:20516722-20516741	None:intergenic	60.0%
	TCACGAAGTGCGACGGTTCC+TGG	-	chr2_4:20516726-20516745	None:intergenic	60.0%
	ACAGCCCGCAAATCCACCGG+CGG	+	chr2_4:20516612-20516631	Msa0320250:CDS	65.0%
	AAATCCGCTCCGGCGACAGG+AGG	+	chr2_4:20516675-20516694	Msa0320250:CDS	65.0%
	AAGCCCCACCGTTTCCGTCC+AGG	+	chr2_4:20516705-20516724	Msa0320250:CDS	65.0%
	CGGTTCCTGGACGGAAACGG+TGG	-	chr2_4:20516713-20516732	None:intergenic	65.0%
!	GCGTGGTGCTTTTCCGCCGG+TGG	-	chr2_4:20516628-20516647	None:intergenic	70.0%
	GGCCGCTCGGAAATCCGCTC+CGG	+	chr2_4:20516665-20516684	Msa0320250:CDS	70.0%
!	CGCCGGAGCGGATTTCCGAG+CGG	-	chr2_4:20516670-20516689	None:intergenic	70.0%
	CGGAAATCCGCTCCGGCGAC+AGG	+	chr2_4:20516672-20516691	Msa0320250:CDS	70.0%
	CACTCCTCCTGTCGCCGGAG+CGG	-	chr2_4:20516682-20516701	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	20516594	20517004	20516594	ID=Msa0320250;Name=Msa0320250
chr2_4	mRNA	20516594	20517004	20516594	ID=Msa0320250-mRNA-1;Parent=Msa0320250;Name=Msa0320250-mRNA-1;_AED=0.43;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
chr2_4	exon	20516594	20517004	20516594	ID=Msa0320250-mRNA-1:exon:7550;Parent=Msa0320250-mRNA-1
chr2_4	CDS	20516594	20517004	20516594	ID=Msa0320250-mRNA-1:cds;Parent=Msa0320250-mRNA-1

Gene Sequence

>Msa0320250

ATGGCACGTACCAAACAAACAGCCCGCAAATCCACCGGCGGAAAAGCACCACGCAAACAACTCGCCACAAAGGCCGCTCGGAAATCCGCTCCGGCGACAGGAGGAGTGAAGAAGCCCCACCGTTTCCGTCCAGGAACCGTCGCACTTCGTGAAATCCGCAAGTACCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCATTTCAAAGATTGGTTCGAGAAATCGCTCAGGATTTCAAAACGGATCTCCGTTTCCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGGGTTACTATTATGCCTAAGGATATTCAGCTTGCGAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>Msa0320250

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*