AlfalfaGEDB | Msa1082020

M. sativa cultivar zhongmu-4 / Msa1082020

KEGG: -
GO: GO:0000302 GO:0001101 GO:0002831 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006950 GO:0006970 GO:0006979 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009625 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010150 GO:0022622 GO:0031347 GO:0031668 GO:0032101 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0042221 GO:0042493 GO:0042542 GO:0042631 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048366 GO:0048367 GO:0048580 GO:0048583 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0080134 GO:0090693 GO:0097305 GO:0099402 GO:0104004 GO:1900055 GO:1900150 GO:1900424 GO:1901700 GO:1901701 GO:1902074 GO:2000024 GO:2000026 GO:2000028 GO:2000241
PFAM: PF03242.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082020	GAU11234.1	82.653	98	11	2	1	93	1	97	3.15e-49	161

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082020	sp\|P32292\|ARG2_VIGRR	53.922	102	35	4	1	93	1	99	2.34e-27	98.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082020	A0A2K3LCK8	82.653	98	11	2	1	93	1	97	1.85e-49	160

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0020410	Msa1082020	0.800467	1.421723e-48	-8.615850e-47
Msa0032080	Msa1082020	0.825755	3.943180e-54	-8.615850e-47
Msa0043980	Msa1082020	0.823193	1.580155e-53	-8.615850e-47
Msa0079070	Msa1082020	0.801619	8.264972e-49	-8.615850e-47
Msa0635080	Msa1082020	0.806082	9.777353e-50	-8.615850e-47
Msa0638540	Msa1082020	0.820027	8.521190e-53	-8.615850e-47
Msa0663920	Msa1082020	0.824737	6.862818e-54	-8.615850e-47
Msa0673470	Msa1082020	0.805902	1.066821e-49	-8.615850e-47
Msa0732880	Msa1082020	0.837173	6.075567e-57	-8.615850e-47
Msa0765180	Msa1082020	0.807829	4.175668e-50	-8.615850e-47
Msa0773850	Msa1082020	0.804623	1.976829e-49	-8.615850e-47
Msa0138020	Msa1082020	0.802145	6.447127e-49	-8.615850e-47
Msa0150230	Msa1082020	0.813359	2.663903e-51	-8.615850e-47
Msa0172080	Msa1082020	0.812262	4.631716e-51	-8.615850e-47
Msa0175840	Msa1082020	0.809426	1.903449e-50	-8.615850e-47
Msa0186950	Msa1082020	0.802225	6.205563e-49	-8.615850e-47
Msa0189030	Msa1082020	0.808194	3.491332e-50	-8.615850e-47
Msa0190250	Msa1082020	0.822325	2.515979e-53	-8.615850e-47
Msa0206010	Msa1082020	0.801641	8.181820e-49	-8.615850e-47
Msa1026640	Msa1082020	0.814955	1.183671e-51	-8.615850e-47
Msa1058750	Msa1082020	0.816130	6.480756e-52	-8.615850e-47
Msa1081910	Msa1082020	0.841355	4.998263e-58	-8.615850e-47
Msa1082020	Msa1090030	0.805543	1.268903e-49	-8.615850e-47
Msa1082020	Msa1107260	0.831981	1.226373e-55	-8.615850e-47
Msa1082020	Msa1114410	0.813124	3.000730e-51	-8.615850e-47
Msa1082020	Msa1150900	0.830702	2.530435e-55	-8.615850e-47
Msa1082020	Msa1174040	0.806567	7.727441e-50	-8.615850e-47
Msa1082020	Msa1270660	0.800076	1.707376e-48	-8.615850e-47
Msa1082020	Msa1335230	0.801787	7.634961e-49	-8.615850e-47
Msa1082020	Msa1337170	0.800914	1.152215e-48	-8.615850e-47
Msa1082020	Msa1407600	0.800688	1.281505e-48	-8.615850e-47
Msa1082020	Msa1437130	0.802968	4.360354e-49	-8.615850e-47
Msa0287410	Msa1082020	0.802555	5.305836e-49	-8.615850e-47
Msa0309270	Msa1082020	0.806479	8.064600e-50	-8.615850e-47
Msa0326110	Msa1082020	0.805710	1.170373e-49	-8.615850e-47
Msa0512750	Msa1082020	0.802571	5.265676e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082020	MtrunA17_Chr7g0269421	82.828	99	11	1	1	93	1	99	2.56e-52	159
Msa1082020	MtrunA17_Chr1g0195391	53.000	100	31	4	1	93	1	91	1.57e-24	88.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082020	AT4G02380.1	45.098	102	38	3	1	90	1	96	9.46e-18	71.6
Msa1082020	AT1G02820.1	48.421	95	39	4	1	90	1	90	6.94e-17	69.3
Msa1082020	AT4G02380.3	45.098	102	38	3	1	90	96	191	1.03e-16	71.2

Find 45 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATATTATATTATGTATTT+TGG	0.081551	7_2:+81946955	Msa1082020:three_prime_UTR
ACTCTTTCATTCATTCTTTC+GGG	0.201868	7_2:+81946389	Msa1082020:five_prime_UTR
AACTCTTTCATTCATTCTTT+CGG	0.219672	7_2:+81946388	Msa1082020:five_prime_UTR
CGGTGGATCTCATGAGCTTT+TGG	0.249055	7_2:+81946992	Msa1082020:three_prime_UTR
CATTCATTCTTTCGGGCTTA+AGG	0.288947	7_2:+81946396	Msa1082020:five_prime_UTR
AGATGGTGCTTCGACTTATA+AGG	0.290073	7_2:+81946798	Msa1082020:CDS
GCAACTAATCATCATTCTTC+TGG	0.297421	7_2:-81947130	None:intergenic
TTCTCTGCTATTGTCCTTGA+AGG	0.342980	7_2:+81946569	Msa1082020:CDS
CTTCGACTTATAAGGTTTCA+TGG	0.345319	7_2:+81946806	Msa1082020:CDS
AAGATGGGACCCACAAAATC+AGG	0.353277	7_2:+81946772	Msa1082020:CDS
CTATAAGCCTGAGAATATTA+AGG	0.363636	7_2:+81946852	Msa1082020:CDS
TACATCTTACACTATTAGTA+TGG	0.374528	7_2:+81946520	Msa1082020:exon
TAGTAACCAGTGACAGGATC+TGG	0.427005	7_2:-81946832	None:intergenic
TCAGTATCCTTAATATTCTC+AGG	0.439065	7_2:-81946859	None:intergenic
AAGATGTATTGAATGAACTA+TGG	0.444802	7_2:-81946505	None:intergenic
TCGTTCTTTCGCTAACGCTA+AGG	0.475487	7_2:+81946544	Msa1082020:CDS
ACGTGGTTACGCTGCGGCAA+GGG	0.477009	7_2:+81946723	Msa1082020:CDS
GGAGGAGTGGTGTCTGTTGT+TGG	0.485348	7_2:+81946745	Msa1082020:CDS
CCCACAAAATCAGGGGAAGA+TGG	0.498300	7_2:+81946781	Msa1082020:CDS
TGGGTTCCAGATCCTGTCAC+TGG	0.502896	7_2:+81946826	Msa1082020:CDS
CGCTGCGGCAAGGGGAGGAG+TGG	0.505897	7_2:+81946732	Msa1082020:CDS
TTGCGAAGATCCTACTCTGT+TGG	0.512688	7_2:+81947064	Msa1082020:three_prime_UTR
TTCGACTTATAAGGTTTCAT+GGG	0.523405	7_2:+81946807	Msa1082020:CDS
TTAACAGAATCAATCAATCA+AGG	0.537130	7_2:-81947041	None:intergenic
GAGTGTTGGAGAATCCTTCA+AGG	0.545395	7_2:-81946583	None:intergenic
GGCTTATAGTAACCAGTGAC+AGG	0.547449	7_2:-81946838	None:intergenic
GTCTGTTGTTGGCAACAAGA+TGG	0.548598	7_2:+81946756	Msa1082020:CDS
GAAGATCCTACTCTGTTGGT+TGG	0.557582	7_2:+81947068	Msa1082020:three_prime_UTR
GACGTGGTTACGCTGCGGCA+AGG	0.563935	7_2:+81946722	Msa1082020:intron
ACGGTACCTTGTGAGAGTGT+TGG	0.566085	7_2:-81946597	None:intergenic
AGATGGGACCCACAAAATCA+GGG	0.576868	7_2:+81946773	Msa1082020:CDS
TCATTCTTTCGGGCTTAAGG+TGG	0.577395	7_2:+81946399	Msa1082020:five_prime_UTR
CGTGGTTACGCTGCGGCAAG+GGG	0.578056	7_2:+81946724	Msa1082020:CDS
TGGGTTTGTTATTTAAACGG+TGG	0.588214	7_2:+81946975	Msa1082020:three_prime_UTR
GTTGCTGATCATAAGTGTAA+TGG	0.599895	7_2:+81947148	Msa1082020:three_prime_UTR
GGTTACGCTGCGGCAAGGGG+AGG	0.607716	7_2:+81946727	Msa1082020:CDS
TCTGTTGTTGGCAACAAGAT+GGG	0.618684	7_2:+81946757	Msa1082020:CDS
AATGAACCAACCAACAGAGT+AGG	0.638709	7_2:-81947074	None:intergenic
CATAACGATAGTTTAAACTG+TGG	0.657037	7_2:+81946431	Msa1082020:five_prime_UTR
AAGCTTAGCACGTAAATCAG+CGG	0.682540	7_2:-81946884	None:intergenic
GATTCTCCAACACTCTCACA+AGG	0.700768	7_2:+81946591	Msa1082020:CDS
GATGGGACCCACAAAATCAG+GGG	0.703081	7_2:+81946774	Msa1082020:CDS
TGATCATAAGTGTAATGGTG+TGG	0.712483	7_2:+81947153	Msa1082020:three_prime_UTR
CTAACAAAGTAAATAAACAG+AGG	0.722024	7_2:-81947097	None:intergenic
AAACAGACGTGGTTACGCTG+CGG	0.738749	7_2:+81946717	Msa1082020:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTATTTTTAATTAATAAT+TGG	+	chr7_2:81947205-81947224	Msa1082020:three_prime_UTR	0.0%
!!!	TTTTATTTTTAATTAATAAT+TGG	+	chr7_2:81947205-81947224	Msa1082020:three_prime_UTR	0.0%
!!!	TTTTAATTAATAATTGGTTT+CGG	+	chr7_2:81947211-81947230	Msa1082020:three_prime_UTR	10.0%
!!!	TTTTAATTAATAATTGGTTT+CGG	+	chr7_2:81947211-81947230	Msa1082020:three_prime_UTR	10.0%
!!!	TTATTTACTTTGTTAGTTTT+TGG	+	chr7_2:81947104-81947123	Msa1082020:three_prime_UTR	15.0%
!!!	TATTTACTTTGTTAGTTTTT+GGG	+	chr7_2:81947105-81947124	Msa1082020:three_prime_UTR	15.0%
!!!	AAAGAGTTTTAAAATTGAAT+GGG	-	chr7_2:81946376-81946395	None:intergenic	15.0%
!!!	TTATTTACTTTGTTAGTTTT+TGG	+	chr7_2:81947104-81947123	Msa1082020:three_prime_UTR	15.0%
!!!	TATTTACTTTGTTAGTTTTT+GGG	+	chr7_2:81947105-81947124	Msa1082020:three_prime_UTR	15.0%
!!	AATAACAATTAGAAACGTTA+CGG	-	chr7_2:81946619-81946638	None:intergenic	20.0%
!!!	ATATCTGAGATTTTAACATA+TGG	+	chr7_2:81946679-81946698	Msa1082020:intron	20.0%
!!	TTAAAATAATTAAACAGACG+TGG	+	chr7_2:81946706-81946725	Msa1082020:intron	20.0%
!!!	ATTAGTTGTTGAATTTTTTG+CGG	-	chr7_2:81946912-81946931	None:intergenic	20.0%
!!!	TTTTGGGTTTGTTATTTAAA+CGG	+	chr7_2:81946972-81946991	Msa1082020:three_prime_UTR	20.0%
!!!	GAAAGAGTTTTAAAATTGAA+TGG	-	chr7_2:81946377-81946396	None:intergenic	20.0%
!!	AATAACAATTAGAAACGTTA+CGG	-	chr7_2:81946619-81946638	None:intergenic	20.0%
!!!	ATATCTGAGATTTTAACATA+TGG	+	chr7_2:81946679-81946698	Msa1082020:intron	20.0%
!!	TTAAAATAATTAAACAGACG+TGG	+	chr7_2:81946706-81946725	Msa1082020:intron	20.0%
!!!	ATTAGTTGTTGAATTTTTTG+CGG	-	chr7_2:81946912-81946931	None:intergenic	20.0%
!!!	TTTTGGGTTTGTTATTTAAA+CGG	+	chr7_2:81946972-81946991	Msa1082020:three_prime_UTR	20.0%
!	AACTCTTTCATTCATTCTTT+CGG	+	chr7_2:81946388-81946407	Msa1082020:five_prime_UTR	25.0%
!	AAGATGTATTGAATGAACTA+TGG	-	chr7_2:81946508-81946527	None:intergenic	25.0%
!	TACATCTTACACTATTAGTA+TGG	+	chr7_2:81946520-81946539	Msa1082020:exon	25.0%
!	TTAACAGAATCAATCAATCA+AGG	-	chr7_2:81947044-81947063	None:intergenic	25.0%
!	CTAACAAAGTAAATAAACAG+AGG	-	chr7_2:81947100-81947119	None:intergenic	25.0%
!	AACTCTTTCATTCATTCTTT+CGG	+	chr7_2:81946388-81946407	Msa1082020:five_prime_UTR	25.0%
!	AAGATGTATTGAATGAACTA+TGG	-	chr7_2:81946508-81946527	None:intergenic	25.0%
!	TACATCTTACACTATTAGTA+TGG	+	chr7_2:81946520-81946539	Msa1082020:exon	25.0%
!	TTAACAGAATCAATCAATCA+AGG	-	chr7_2:81947044-81947063	None:intergenic	25.0%
!	CTAACAAAGTAAATAAACAG+AGG	-	chr7_2:81947100-81947119	None:intergenic	25.0%
	ACTCTTTCATTCATTCTTTC+GGG	+	chr7_2:81946389-81946408	Msa1082020:five_prime_UTR	30.0%
	CATAACGATAGTTTAAACTG+TGG	+	chr7_2:81946431-81946450	Msa1082020:five_prime_UTR	30.0%
	TTCGACTTATAAGGTTTCAT+GGG	+	chr7_2:81946807-81946826	Msa1082020:CDS	30.0%
	CTATAAGCCTGAGAATATTA+AGG	+	chr7_2:81946852-81946871	Msa1082020:CDS	30.0%
	TCAGTATCCTTAATATTCTC+AGG	-	chr7_2:81946862-81946881	None:intergenic	30.0%
	ACTCTTTCATTCATTCTTTC+GGG	+	chr7_2:81946389-81946408	Msa1082020:five_prime_UTR	30.0%
	CATAACGATAGTTTAAACTG+TGG	+	chr7_2:81946431-81946450	Msa1082020:five_prime_UTR	30.0%
	TTCGACTTATAAGGTTTCAT+GGG	+	chr7_2:81946807-81946826	Msa1082020:CDS	30.0%
	CTATAAGCCTGAGAATATTA+AGG	+	chr7_2:81946852-81946871	Msa1082020:CDS	30.0%
	TCAGTATCCTTAATATTCTC+AGG	-	chr7_2:81946862-81946881	None:intergenic	30.0%
	CTTCGACTTATAAGGTTTCA+TGG	+	chr7_2:81946806-81946825	Msa1082020:CDS	35.0%
!!	TGGGTTTGTTATTTAAACGG+TGG	+	chr7_2:81946975-81946994	Msa1082020:three_prime_UTR	35.0%
!!!	CTTTTGGGATCGATTTTAGA+TGG	+	chr7_2:81947008-81947027	Msa1082020:three_prime_UTR	35.0%
	GCAACTAATCATCATTCTTC+TGG	-	chr7_2:81947133-81947152	None:intergenic	35.0%
	GTTGCTGATCATAAGTGTAA+TGG	+	chr7_2:81947148-81947167	Msa1082020:three_prime_UTR	35.0%
	TGATCATAAGTGTAATGGTG+TGG	+	chr7_2:81947153-81947172	Msa1082020:three_prime_UTR	35.0%
	CTTCGACTTATAAGGTTTCA+TGG	+	chr7_2:81946806-81946825	Msa1082020:CDS	35.0%
!!	TGGGTTTGTTATTTAAACGG+TGG	+	chr7_2:81946975-81946994	Msa1082020:three_prime_UTR	35.0%
!!!	CTTTTGGGATCGATTTTAGA+TGG	+	chr7_2:81947008-81947027	Msa1082020:three_prime_UTR	35.0%
	GCAACTAATCATCATTCTTC+TGG	-	chr7_2:81947133-81947152	None:intergenic	35.0%
	GTTGCTGATCATAAGTGTAA+TGG	+	chr7_2:81947148-81947167	Msa1082020:three_prime_UTR	35.0%
	TGATCATAAGTGTAATGGTG+TGG	+	chr7_2:81947153-81947172	Msa1082020:three_prime_UTR	35.0%
	CATTCATTCTTTCGGGCTTA+AGG	+	chr7_2:81946396-81946415	Msa1082020:five_prime_UTR	40.0%
	TTCTCTGCTATTGTCCTTGA+AGG	+	chr7_2:81946569-81946588	Msa1082020:CDS	40.0%
	TCTGTTGTTGGCAACAAGAT+GGG	+	chr7_2:81946757-81946776	Msa1082020:CDS	40.0%
	AGATGGTGCTTCGACTTATA+AGG	+	chr7_2:81946798-81946817	Msa1082020:CDS	40.0%
	AAGCTTAGCACGTAAATCAG+CGG	-	chr7_2:81946887-81946906	None:intergenic	40.0%
	AATGAACCAACCAACAGAGT+AGG	-	chr7_2:81947077-81947096	None:intergenic	40.0%
	CATTCATTCTTTCGGGCTTA+AGG	+	chr7_2:81946396-81946415	Msa1082020:five_prime_UTR	40.0%
	TTCTCTGCTATTGTCCTTGA+AGG	+	chr7_2:81946569-81946588	Msa1082020:CDS	40.0%
	TCTGTTGTTGGCAACAAGAT+GGG	+	chr7_2:81946757-81946776	Msa1082020:CDS	40.0%
	AGATGGTGCTTCGACTTATA+AGG	+	chr7_2:81946798-81946817	Msa1082020:CDS	40.0%
	AAGCTTAGCACGTAAATCAG+CGG	-	chr7_2:81946887-81946906	None:intergenic	40.0%
	AATGAACCAACCAACAGAGT+AGG	-	chr7_2:81947077-81947096	None:intergenic	40.0%
	TCATTCTTTCGGGCTTAAGG+TGG	+	chr7_2:81946399-81946418	Msa1082020:five_prime_UTR	45.0%
	TCGTTCTTTCGCTAACGCTA+AGG	+	chr7_2:81946544-81946563	Msa1082020:CDS	45.0%
!!	GAGTGTTGGAGAATCCTTCA+AGG	-	chr7_2:81946586-81946605	None:intergenic	45.0%
	GATTCTCCAACACTCTCACA+AGG	+	chr7_2:81946591-81946610	Msa1082020:CDS	45.0%
	GTCTGTTGTTGGCAACAAGA+TGG	+	chr7_2:81946756-81946775	Msa1082020:CDS	45.0%
	AAGATGGGACCCACAAAATC+AGG	+	chr7_2:81946772-81946791	Msa1082020:CDS	45.0%
	AGATGGGACCCACAAAATCA+GGG	+	chr7_2:81946773-81946792	Msa1082020:CDS	45.0%
!	CCATCTTCCCCTGATTTTGT+GGG	-	chr7_2:81946784-81946803	None:intergenic	45.0%
!	ACCATCTTCCCCTGATTTTG+TGG	-	chr7_2:81946785-81946804	None:intergenic	45.0%
	TAGTAACCAGTGACAGGATC+TGG	-	chr7_2:81946835-81946854	None:intergenic	45.0%
	GGCTTATAGTAACCAGTGAC+AGG	-	chr7_2:81946841-81946860	None:intergenic	45.0%
!	GGTGGATCTCATGAGCTTTT+GGG	+	chr7_2:81946993-81947012	Msa1082020:three_prime_UTR	45.0%
	TTGCGAAGATCCTACTCTGT+TGG	+	chr7_2:81947064-81947083	Msa1082020:three_prime_UTR	45.0%
	GAAGATCCTACTCTGTTGGT+TGG	+	chr7_2:81947068-81947087	Msa1082020:three_prime_UTR	45.0%
	TCATTCTTTCGGGCTTAAGG+TGG	+	chr7_2:81946399-81946418	Msa1082020:five_prime_UTR	45.0%
	TCGTTCTTTCGCTAACGCTA+AGG	+	chr7_2:81946544-81946563	Msa1082020:CDS	45.0%
!!	GAGTGTTGGAGAATCCTTCA+AGG	-	chr7_2:81946586-81946605	None:intergenic	45.0%
	GATTCTCCAACACTCTCACA+AGG	+	chr7_2:81946591-81946610	Msa1082020:CDS	45.0%
	GTCTGTTGTTGGCAACAAGA+TGG	+	chr7_2:81946756-81946775	Msa1082020:CDS	45.0%
	AAGATGGGACCCACAAAATC+AGG	+	chr7_2:81946772-81946791	Msa1082020:CDS	45.0%
	AGATGGGACCCACAAAATCA+GGG	+	chr7_2:81946773-81946792	Msa1082020:CDS	45.0%
!	CCATCTTCCCCTGATTTTGT+GGG	-	chr7_2:81946784-81946803	None:intergenic	45.0%
!	ACCATCTTCCCCTGATTTTG+TGG	-	chr7_2:81946785-81946804	None:intergenic	45.0%
	TAGTAACCAGTGACAGGATC+TGG	-	chr7_2:81946835-81946854	None:intergenic	45.0%
	GGCTTATAGTAACCAGTGAC+AGG	-	chr7_2:81946841-81946860	None:intergenic	45.0%
!	GGTGGATCTCATGAGCTTTT+GGG	+	chr7_2:81946993-81947012	Msa1082020:three_prime_UTR	45.0%
	TTGCGAAGATCCTACTCTGT+TGG	+	chr7_2:81947064-81947083	Msa1082020:three_prime_UTR	45.0%
	GAAGATCCTACTCTGTTGGT+TGG	+	chr7_2:81947068-81947087	Msa1082020:three_prime_UTR	45.0%
!!!	TTATATTATATTATGTATTT+TGG	+	chr7_2:81946955-81946974	Msa1082020:three_prime_UTR	5.0%
!!!	TATATTATATTATGTATTTT+GGG	+	chr7_2:81946956-81946975	Msa1082020:three_prime_UTR	5.0%
!!!	TTATATTATATTATGTATTT+TGG	+	chr7_2:81946955-81946974	Msa1082020:three_prime_UTR	5.0%
!!!	TATATTATATTATGTATTTT+GGG	+	chr7_2:81946956-81946975	Msa1082020:three_prime_UTR	5.0%
!	ACGGTACCTTGTGAGAGTGT+TGG	-	chr7_2:81946600-81946619	None:intergenic	50.0%
	AAACAGACGTGGTTACGCTG+CGG	+	chr7_2:81946717-81946736	Msa1082020:intron	50.0%
	GATGGGACCCACAAAATCAG+GGG	+	chr7_2:81946774-81946793	Msa1082020:CDS	50.0%
	CCCACAAAATCAGGGGAAGA+TGG	+	chr7_2:81946781-81946800	Msa1082020:CDS	50.0%
!	CGGTGGATCTCATGAGCTTT+TGG	+	chr7_2:81946992-81947011	Msa1082020:three_prime_UTR	50.0%
!	ACGGTACCTTGTGAGAGTGT+TGG	-	chr7_2:81946600-81946619	None:intergenic	50.0%
	AAACAGACGTGGTTACGCTG+CGG	+	chr7_2:81946717-81946736	Msa1082020:intron	50.0%
	GATGGGACCCACAAAATCAG+GGG	+	chr7_2:81946774-81946793	Msa1082020:CDS	50.0%
	CCCACAAAATCAGGGGAAGA+TGG	+	chr7_2:81946781-81946800	Msa1082020:CDS	50.0%
!	CGGTGGATCTCATGAGCTTT+TGG	+	chr7_2:81946992-81947011	Msa1082020:three_prime_UTR	50.0%
	GGAGGAGTGGTGTCTGTTGT+TGG	+	chr7_2:81946745-81946764	Msa1082020:CDS	55.0%
	TGGGTTCCAGATCCTGTCAC+TGG	+	chr7_2:81946826-81946845	Msa1082020:CDS	55.0%
	GGAGGAGTGGTGTCTGTTGT+TGG	+	chr7_2:81946745-81946764	Msa1082020:CDS	55.0%
	TGGGTTCCAGATCCTGTCAC+TGG	+	chr7_2:81946826-81946845	Msa1082020:CDS	55.0%
	ACGTGGTTACGCTGCGGCAA+GGG	+	chr7_2:81946723-81946742	Msa1082020:CDS	60.0%
	ACGTGGTTACGCTGCGGCAA+GGG	+	chr7_2:81946723-81946742	Msa1082020:CDS	60.0%
	GACGTGGTTACGCTGCGGCA+AGG	+	chr7_2:81946722-81946741	Msa1082020:intron	65.0%
	CGTGGTTACGCTGCGGCAAG+GGG	+	chr7_2:81946724-81946743	Msa1082020:CDS	65.0%
	GACGTGGTTACGCTGCGGCA+AGG	+	chr7_2:81946722-81946741	Msa1082020:intron	65.0%
	CGTGGTTACGCTGCGGCAAG+GGG	+	chr7_2:81946724-81946743	Msa1082020:CDS	65.0%
	GGTTACGCTGCGGCAAGGGG+AGG	+	chr7_2:81946727-81946746	Msa1082020:CDS	70.0%
	GGTTACGCTGCGGCAAGGGG+AGG	+	chr7_2:81946727-81946746	Msa1082020:CDS	70.0%
	CGCTGCGGCAAGGGGAGGAG+TGG	+	chr7_2:81946732-81946751	Msa1082020:CDS	75.0%
	CGCTGCGGCAAGGGGAGGAG+TGG	+	chr7_2:81946732-81946751	Msa1082020:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	81946376	81947267	81946376	ID=Msa1082020;Name=Msa1082020
chr7_2	mRNA	81946376	81947267	81946376	ID=Msa1082020-mRNA-1;Parent=Msa1082020;Name=Msa1082020-mRNA-1;_AED=0.03;_eAED=0.03;_QI=163\|1\|1\|1\|1\|1\|2\|337\|93
chr7_2	exon	81946376	81946612	81946376	ID=Msa1082020-mRNA-1:exon:21098;Parent=Msa1082020-mRNA-1
chr7_2	exon	81946723	81947267	81946723	ID=Msa1082020-mRNA-1:exon:21099;Parent=Msa1082020-mRNA-1
chr7_2	five_prime_UTR	81946376	81946538	81946376	ID=Msa1082020-mRNA-1:five_prime_utr;Parent=Msa1082020-mRNA-1
chr7_2	CDS	81946539	81946612	81946539	ID=Msa1082020-mRNA-1:cds;Parent=Msa1082020-mRNA-1
chr7_2	CDS	81946723	81946930	81946723	ID=Msa1082020-mRNA-1:cds;Parent=Msa1082020-mRNA-1
chr7_2	three_prime_UTR	81946931	81947267	81946931	ID=Msa1082020-mRNA-1:three_prime_utr;Parent=Msa1082020-mRNA-1

Gene Sequence	>Msa1082020 ATGGCTCGTTCTTTCGCTAACGCTAAGGCTTTCTCTGCTATTGTCCTTGAAGGATTCTCCAACACTCTCACAAGACGTGGTTACGCTGCGGCAAGGGGAGGAGTGGTGTCTGTTGTTGGCAACAAGATGGGACCCACAAAATCAGGGGAAGATGGTGCTTCGACTTATAAGGTTTCATGGGTTCCAGATCCTGTCACTGGTTACTATAAGCCTGAGAATATTAAGGATACTGATGCCGCTGATTTACGTGCTAAGCTTCTCCGCAAAAAATTCAACAACTAA
Protein sequence	>Msa1082020 MARSFANAKAFSAIVLEGFSNTLTRRGYAAARGGVVSVVGNKMGPTKSGEDGASTYKVSWVPDPVTGYYKPENIKDTDAADLRAKLLRKKFNN*