AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437130 MtrunA17_Chr7g0217561 99.078 217 2 0 1 217 1 217 3.95e-159 438
Msa1437130 MtrunA17_Chr6g0453281 63.713 237 66 4 1 217 1 237 1.21e-99 288
Msa1437130 MtrunA17_Chr2g0287451 42.574 101 46 2 120 217 103 194 9.01e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437130 AT3G01170.1 51.339 224 93 5 1 217 1 215 7.15e-67 205
Msa1437130 AT5G15260.2 51.261 238 91 8 1 217 1 234 9.30e-67 205
Msa1437130 AT5G15260.1 51.261 238 91 8 1 217 1 234 9.30e-67 205
Msa1437130 AT5G19025.1 54.082 98 43 1 120 217 150 245 9.54e-26 100
Msa1437130 AT5G19025.2 53.608 97 43 1 120 216 150 244 4.25e-25 99.4
Msa1437130 AT3G06180.1 53.061 98 44 1 120 217 132 227 7.28e-25 98.2
Msa1437130 AT3G01170.2 39.216 102 56 2 1 97 1 101 2.16e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTGTTGTTATTGCTTT+AGG 0.138895 tig0028150:-20514 Msa1437130:CDS
CAAACTTTGTGGTTTGTTTA+TGG 0.153027 tig0028150:-20446 Msa1437130:CDS
TTGATCTTTCTATTCTTCTT+TGG 0.201359 tig0028150:+20052 None:intergenic
TGTTATTGCTTTAGGGTTCT+TGG 0.201503 tig0028150:-20506 Msa1437130:CDS
TAGCTGTTGTTATTGCTTTA+GGG 0.217546 tig0028150:-20513 Msa1437130:CDS
TTCTTCAATCCCTTACAATT+AGG 0.224249 tig0028150:+20301 None:intergenic
GAATAGAAAGATCAAGAAAT+AGG 0.263550 tig0028150:-20044 None:intergenic
CTCATCACAAATTGAATATA+TGG 0.264029 tig0028150:+20478 None:intergenic
TCTTCAATCCCTTACAATTA+GGG 0.277107 tig0028150:+20302 None:intergenic
TGTTGGTAGGTTAGAAGTTC+CGG 0.283392 tig0028150:-20077 Msa1437130:CDS
TGTGAAGAATTCGCCGTCTT+TGG 0.288388 tig0028150:-20239 Msa1437130:CDS
AAGAAGATGGCTCCTGTTAA+TGG 0.295596 tig0028150:-20139 Msa1437130:CDS
AGATGGTGGTGGAATTAAAA+AGG 0.303114 tig0028150:-20212 Msa1437130:CDS
TTGTGGTTTGTTTATGGATT+TGG 0.314324 tig0028150:-20440 Msa1437130:CDS
CATGTGCTGTTGTTGCTACA+TGG 0.391115 tig0028150:-20360 Msa1437130:CDS
GTTGGTAGGTTAGAAGTTCC+GGG 0.396075 tig0028150:-20076 Msa1437130:CDS
AGTGAAAGTTTCAAACTTTG+TGG 0.397466 tig0028150:-20457 Msa1437130:CDS
CCTGTGATTTATGTATCTCT+TGG 0.405703 tig0028150:-20394 Msa1437130:CDS
AGAACAGCTCTTCCATTAAC+AGG 0.409782 tig0028150:+20127 None:intergenic
GTGAAGAATTCGCCGTCTTT+GGG 0.417873 tig0028150:-20238 Msa1437130:CDS
ATGTGGGAACCCTAATTGTA+AGG 0.424650 tig0028150:-20311 Msa1437130:CDS
AGAGCTGTTCTAGTTCTTAG+AGG 0.448409 tig0028150:-20115 Msa1437130:CDS
CAACAGATGAGATCAAACTA+TGG 0.452999 tig0028150:+20665 None:intergenic
AGTGTGGTTGTTCTGTTGGT+AGG 0.472839 tig0028150:-20090 Msa1437130:CDS
GGAAAGTGTGGTTGTTCTGT+TGG 0.477218 tig0028150:-20094 Msa1437130:CDS
GAAGAGAATTGAGTGTCTTA+CGG 0.499754 tig0028150:+20590 None:intergenic
TCGATCCGCGGTTGTTGATG+TGG 0.500037 tig0028150:-20545 Msa1437130:CDS
CTTATACATCAAGGAAATGT+GGG 0.514031 tig0028150:-20327 Msa1437130:CDS
TTTCTATTCTTCTTTGGACC+CGG 0.518411 tig0028150:+20058 None:intergenic
CGAAGCCGAGCTTAAGAAGA+TGG 0.524432 tig0028150:-20152 Msa1437130:CDS
AGGAGCCATCTTCTTAAGCT+CGG 0.536872 tig0028150:+20147 None:intergenic
GCTTATACATCAAGGAAATG+TGG 0.544775 tig0028150:-20328 Msa1437130:CDS
TGTGGGAACCCTAATTGTAA+GGG 0.554639 tig0028150:-20310 Msa1437130:CDS
TCGCCATGGATGATGATGAT+TGG 0.560084 tig0028150:+20641 None:intergenic
TGCTGAGTTTGATATTCAGT+TGG 0.575183 tig0028150:-20275 Msa1437130:CDS
CTAGTTCTTAGAGGAAAGTG+TGG 0.579987 tig0028150:-20106 Msa1437130:CDS
TCCTCGATTTAGCTAAATCG+AGG 0.583685 tig0028150:+20614 None:intergenic
CCGTCTTTGGGTAAAGATGG+TGG 0.586722 tig0028150:-20226 Msa1437130:CDS
CCAAGAGATACATAAATCAC+AGG 0.588766 tig0028150:+20394 None:intergenic
CCCTCGATTTAGCTAAATCG+AGG 0.592975 tig0028150:-20615 Msa1437130:CDS
TGACCAATCATCATCATCCA+TGG 0.601187 tig0028150:-20644 Msa1437130:CDS
CCTCGATTTAGCTAAATCGA+GGG 0.621163 tig0028150:+20615 None:intergenic
CCACCATCTTTACCCAAAGA+CGG 0.627811 tig0028150:+20226 None:intergenic
GATCGAGATCGCTCGCACTG+CGG 0.651752 tig0028150:+20562 None:intergenic
AGTTCGCGATGATGATCACA+AGG 0.663527 tig0028150:+20175 None:intergenic
TCTTTGGGTAAAGATGGTGG+TGG 0.667680 tig0028150:-20223 Msa1437130:CDS
TAAATCGAGGGAATTCGCCA+TGG 0.679775 tig0028150:+20627 None:intergenic
TATAACCACATCAACAACCG+CGG 0.698235 tig0028150:+20540 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAGCTTATACATCA+AGG - tig0028150:20385-20404 Msa1437130:CDS 20.0%
! CTCATCACAAATTGAATATA+TGG + tig0028150:20246-20265 None:intergenic 25.0%
! TTGATCTTTCTATTCTTCTT+TGG + tig0028150:20672-20691 None:intergenic 25.0%
! ATAGCTGTTGTTATTGCTTT+AGG - tig0028150:20207-20226 Msa1437130:CDS 30.0%
! TAGCTGTTGTTATTGCTTTA+GGG - tig0028150:20208-20227 Msa1437130:CDS 30.0%
AGTGAAAGTTTCAAACTTTG+TGG - tig0028150:20264-20283 Msa1437130:CDS 30.0%
!! CAAACTTTGTGGTTTGTTTA+TGG - tig0028150:20275-20294 Msa1437130:CDS 30.0%
!! TTGTGGTTTGTTTATGGATT+TGG - tig0028150:20281-20300 Msa1437130:CDS 30.0%
CTTATACATCAAGGAAATGT+GGG - tig0028150:20394-20413 Msa1437130:CDS 30.0%
TCTTCAATCCCTTACAATTA+GGG + tig0028150:20422-20441 None:intergenic 30.0%
TTCTTCAATCCCTTACAATT+AGG + tig0028150:20423-20442 None:intergenic 30.0%
CAACAGATGAGATCAAACTA+TGG + tig0028150:20059-20078 None:intergenic 35.0%
GAAGAGAATTGAGTGTCTTA+CGG + tig0028150:20134-20153 None:intergenic 35.0%
!! TGTTATTGCTTTAGGGTTCT+TGG - tig0028150:20215-20234 Msa1437130:CDS 35.0%
CCAAGAGATACATAAATCAC+AGG + tig0028150:20330-20349 None:intergenic 35.0%
CCTGTGATTTATGTATCTCT+TGG - tig0028150:20327-20346 Msa1437130:CDS 35.0%
GCTTATACATCAAGGAAATG+TGG - tig0028150:20393-20412 Msa1437130:CDS 35.0%
TGCTGAGTTTGATATTCAGT+TGG - tig0028150:20446-20465 Msa1437130:CDS 35.0%
!! AGATGGTGGTGGAATTAAAA+AGG - tig0028150:20509-20528 Msa1437130:CDS 35.0%
!! GATGGTGGTGGAATTAAAAA+GGG - tig0028150:20510-20529 Msa1437130:CDS 35.0%
TTTCTATTCTTCTTTGGACC+CGG + tig0028150:20666-20685 None:intergenic 35.0%
TGACCAATCATCATCATCCA+TGG - tig0028150:20077-20096 Msa1437130:CDS 40.0%
CCTCGATTTAGCTAAATCGA+GGG + tig0028150:20107-20126 None:intergenic 40.0%
TCCTCGATTTAGCTAAATCG+AGG + tig0028150:20110-20129 None:intergenic 40.0%
TATAACCACATCAACAACCG+CGG + tig0028150:20184-20203 None:intergenic 40.0%
ATGTGGGAACCCTAATTGTA+AGG - tig0028150:20410-20429 Msa1437130:CDS 40.0%
TGTGGGAACCCTAATTGTAA+GGG - tig0028150:20411-20430 Msa1437130:CDS 40.0%
AAGAAGATGGCTCCTGTTAA+TGG - tig0028150:20582-20601 Msa1437130:CDS 40.0%
AGAACAGCTCTTCCATTAAC+AGG + tig0028150:20597-20616 None:intergenic 40.0%
!! AGAGCTGTTCTAGTTCTTAG+AGG - tig0028150:20606-20625 Msa1437130:CDS 40.0%
! CTAGTTCTTAGAGGAAAGTG+TGG - tig0028150:20615-20634 Msa1437130:CDS 40.0%
! TGTTGGTAGGTTAGAAGTTC+CGG - tig0028150:20644-20663 Msa1437130:CDS 40.0%
! TCGCCATGGATGATGATGAT+TGG + tig0028150:20083-20102 None:intergenic 45.0%
TAAATCGAGGGAATTCGCCA+TGG + tig0028150:20097-20116 None:intergenic 45.0%
CCCTCGATTTAGCTAAATCG+AGG - tig0028150:20106-20125 Msa1437130:CDS 45.0%
! CATGTGCTGTTGTTGCTACA+TGG - tig0028150:20361-20380 Msa1437130:CDS 45.0%
TGTGAAGAATTCGCCGTCTT+TGG - tig0028150:20482-20501 Msa1437130:CDS 45.0%
GTGAAGAATTCGCCGTCTTT+GGG - tig0028150:20483-20502 Msa1437130:CDS 45.0%
CCACCATCTTTACCCAAAGA+CGG + tig0028150:20498-20517 None:intergenic 45.0%
! TCTTTGGGTAAAGATGGTGG+TGG - tig0028150:20498-20517 Msa1437130:CDS 45.0%
AGTTCGCGATGATGATCACA+AGG + tig0028150:20549-20568 None:intergenic 45.0%
AGGAGCCATCTTCTTAAGCT+CGG + tig0028150:20577-20596 None:intergenic 45.0%
! GGAAAGTGTGGTTGTTCTGT+TGG - tig0028150:20627-20646 Msa1437130:CDS 45.0%
AGTGTGGTTGTTCTGTTGGT+AGG - tig0028150:20631-20650 Msa1437130:CDS 45.0%
! GTTGGTAGGTTAGAAGTTCC+GGG - tig0028150:20645-20664 Msa1437130:CDS 45.0%
TCGCCGTCTTTGGGTAAAGA+TGG - tig0028150:20492-20511 Msa1437130:CDS 50.0%
CCGTCTTTGGGTAAAGATGG+TGG - tig0028150:20495-20514 Msa1437130:CDS 50.0%
CGAAGCCGAGCTTAAGAAGA+TGG - tig0028150:20569-20588 Msa1437130:CDS 50.0%
TCGATCCGCGGTTGTTGATG+TGG - tig0028150:20176-20195 Msa1437130:CDS 55.0%
GATCGAGATCGCTCGCACTG+CGG + tig0028150:20162-20181 None:intergenic 60.0%
GTGCGAGCGATCTCGATCCG+CGG - tig0028150:20164-20183 Msa1437130:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0028150 gene 20045 20698 20045 ID=Msa1437130;Name=Msa1437130
tig0028150 mRNA 20045 20698 20045 ID=Msa1437130-mRNA-1;Parent=Msa1437130;Name=Msa1437130-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|217
tig0028150 exon 20045 20698 20045 ID=Msa1437130-mRNA-1:exon:10500;Parent=Msa1437130-mRNA-1
tig0028150 CDS 20045 20698 20045 ID=Msa1437130-mRNA-1:cds;Parent=Msa1437130-mRNA-1
Gene Sequence

>Msa1437130

ATGGTAAATTTCCATAGTTTGATCTCATCTGTTGACCAATCATCATCATCCATGGCGAATTCCCTCGATTTAGCTAAATCGAGGAACCGTAAGACACTCAATTCTCTTCAGATTCCGCAGTGCGAGCGATCTCGATCCGCGGTTGTTGATGTGGTTATATTTATAGCTGTTGTTATTGCTTTAGGGTTCTTGGTTTTTCCATATATTCAATTTGTGATGAGTGAAAGTTTCAAACTTTGTGGTTTGTTTATGGATTTGGTGAAAGAAGAAGTTGCTGTTGCTCCTGTGATTTATGTATCTCTTGGAGTTAGTGTTTCATGTGCTGTTGTTGCTACATGGTTTTTTATAGCTTATACATCAAGGAAATGTGGGAACCCTAATTGTAAGGGATTGAAGAATGCTGCTGAGTTTGATATTCAGTTGGAAACTGAAGATTGTGTGAAGAATTCGCCGTCTTTGGGTAAAGATGGTGGTGGAATTAAAAAGGGTCTTTTTAAGATTCCTTGTGATCATCATCGCGAACTCGAAGCCGAGCTTAAGAAGATGGCTCCTGTTAATGGAAGAGCTGTTCTAGTTCTTAGAGGAAAGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGTCCAAAGAAGAATAGAAAGATCAAGAAATAG

Protein sequence

>Msa1437130

MVNFHSLISSVDQSSSSMANSLDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVVIALGFLVFPYIQFVMSESFKLCGLFMDLVKEEVAVAPVIYVSLGVSVSCAVVATWFFIAYTSRKCGNPNCKGLKNAAEFDIQLETEDCVKNSPSLGKDGGGIKKGLFKIPCDHHRELEAELKKMAPVNGRAVLVLRGKCGCSVGRLEVPGPKKNRKIKK*