AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0773850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773850 MtrunA17_Chr5g0427841 93.407 91 6 0 1 91 1 91 1.63e-54 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773850 AT2G38465.1 59.420 69 28 0 19 87 13 81 2.67e-22 82.8

Find 19 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCACAACAAAACTTCTTT+TGG 0.285814 5_2:+49210453 Msa0773850:CDS
GTATCTTCATATTGTTTGTC+TGG 0.325802 5_2:+49210338 Msa0773850:five_prime_UTR
GATATATATTTCATCTCTTA+AGG 0.348244 5_2:+49210377 Msa0773850:five_prime_UTR
CATTAATTTAATTAACTATG+AGG 0.404180 5_2:-49210796 None:intergenic
CTGCTGAACATATCAAGAAT+AGG 0.429170 5_2:+49210764 Msa0773850:CDS
GTCTCTGAACAATTGCTAGA+TGG 0.431183 5_2:+49210221 None:intergenic
AAAAGCCTTCTTTGTGGGTC+AGG 0.435149 5_2:+49210539 Msa0773850:CDS
TATGCAAAAGCCTTCTTTGT+GGG 0.462187 5_2:+49210534 Msa0773850:CDS
AAAAGTAGCTATGGAAGAGA+TGG 0.471774 5_2:+49210404 Msa0773850:exon
TAAGGCAATAAAAGTAGCTA+TGG 0.496433 5_2:+49210395 Msa0773850:exon
ATATGCAAAAGCCTTCTTTG+TGG 0.519860 5_2:+49210533 Msa0773850:CDS
AATTACCTGACCCACAAAGA+AGG 0.535666 5_2:-49210544 None:intergenic
TTTCTATAAAAGAAGGACAA+TGG 0.565158 5_2:+49210305 Msa0773850:five_prime_UTR
AAAGTAGCTATGGAAGAGAT+GGG 0.580832 5_2:+49210405 Msa0773850:exon
TGTCTGGACACACACAAACA+AGG 0.587340 5_2:+49210354 Msa0773850:five_prime_UTR
GGCTTCAAAGAAACAAGTTG+AGG 0.622095 5_2:+49210732 Msa0773850:CDS
AGCAAGTGAAGCTGAACTTG+AGG 0.634101 5_2:+49210711 Msa0773850:CDS
GTCTGGACACACACAAACAA+GGG 0.643617 5_2:+49210355 Msa0773850:five_prime_UTR
AGAATCTTAGAAGACTTACA+AGG 0.654761 5_2:-49210265 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTATTTTTCTATAAAAGA+AGG + chr5_2:49210298-49210317 Msa0773850:five_prime_UTR 15.0%
!! AAAAATTTCTTGTATAGAAA+CGG - chr5_2:49210511-49210530 None:intergenic 15.0%
!! CATTAATTTAATTAACTATG+AGG - chr5_2:49210799-49210818 None:intergenic 15.0%
!! GATATATATTTCATCTCTTA+AGG + chr5_2:49210377-49210396 Msa0773850:five_prime_UTR 20.0%
! TTTCTATAAAAGAAGGACAA+TGG + chr5_2:49210305-49210324 Msa0773850:five_prime_UTR 25.0%
!!! AAAGAAGTTTTGTTGTGATT+TGG - chr5_2:49210453-49210472 None:intergenic 25.0%
!! AATCACAACAAAACTTCTTT+TGG + chr5_2:49210453-49210472 Msa0773850:CDS 25.0%
! AAATAAGTAGTAGCGAAAAA+TGG - chr5_2:49210593-49210612 None:intergenic 25.0%
! TATAACTGCAAAAAATGAGA+AGG + chr5_2:49210687-49210706 Msa0773850:CDS 25.0%
AGAATCTTAGAAGACTTACA+AGG - chr5_2:49210268-49210287 None:intergenic 30.0%
GTATCTTCATATTGTTTGTC+TGG + chr5_2:49210338-49210357 Msa0773850:five_prime_UTR 30.0%
TAAGGCAATAAAAGTAGCTA+TGG + chr5_2:49210395-49210414 Msa0773850:exon 30.0%
TGTGGTGTGGAAAAAAAAAT+AGG + chr5_2:49210657-49210676 Msa0773850:intron 30.0%
AAAAGAAAAACACTTGCATG+AGG - chr5_2:49210896-49210915 None:intergenic 30.0%
! ATGCAAGTGTTTTTCTTTTC+TGG + chr5_2:49210897-49210916 Msa0773850:three_prime_UTR 30.0%
AAAAGTAGCTATGGAAGAGA+TGG + chr5_2:49210404-49210423 Msa0773850:exon 35.0%
AAAGTAGCTATGGAAGAGAT+GGG + chr5_2:49210405-49210424 Msa0773850:exon 35.0%
ATATGCAAAAGCCTTCTTTG+TGG + chr5_2:49210533-49210552 Msa0773850:CDS 35.0%
TATGCAAAAGCCTTCTTTGT+GGG + chr5_2:49210534-49210553 Msa0773850:CDS 35.0%
!! AAATGGAAAGGGGTTGTTTT+TGG - chr5_2:49210576-49210595 None:intergenic 35.0%
TAGTAGCGAAAAATGGAAAG+GGG - chr5_2:49210586-49210605 None:intergenic 35.0%
GTAGTAGCGAAAAATGGAAA+GGG - chr5_2:49210587-49210606 None:intergenic 35.0%
AGTAGTAGCGAAAAATGGAA+AGG - chr5_2:49210588-49210607 None:intergenic 35.0%
!! GAAATTGCCTTGTTTGATTG+AGG + chr5_2:49210631-49210650 Msa0773850:intron 35.0%
CACAATTCCTCAATCAAACA+AGG - chr5_2:49210641-49210660 None:intergenic 35.0%
CTTGTTTGATTGAGGAATTG+TGG + chr5_2:49210639-49210658 Msa0773850:intron 35.0%
CTGCTGAACATATCAAGAAT+AGG + chr5_2:49210764-49210783 Msa0773850:CDS 35.0%
AATTACCTGACCCACAAAGA+AGG - chr5_2:49210547-49210566 None:intergenic 40.0%
TTGATTGAGGAATTGTGGTG+TGG + chr5_2:49210644-49210663 Msa0773850:intron 40.0%
GGCTTCAAAGAAACAAGTTG+AGG + chr5_2:49210732-49210751 Msa0773850:CDS 40.0%
TGTCTGGACACACACAAACA+AGG + chr5_2:49210354-49210373 Msa0773850:five_prime_UTR 45.0%
GTCTGGACACACACAAACAA+GGG + chr5_2:49210355-49210374 Msa0773850:five_prime_UTR 45.0%
AAAAGCCTTCTTTGTGGGTC+AGG + chr5_2:49210539-49210558 Msa0773850:CDS 45.0%
AGCAAGTGAAGCTGAACTTG+AGG + chr5_2:49210711-49210730 Msa0773850:CDS 45.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 49210224 49210957 49210224 ID=Msa0773850;Name=Msa0773850
chr5_2 mRNA 49210224 49210957 49210224 ID=Msa0773850-mRNA-1;Parent=Msa0773850;Name=Msa0773850-mRNA-1;_AED=0.00;_eAED=0.00;_QI=190|1|1|1|1|1|2|150|91
chr5_2 exon 49210224 49210560 49210224 ID=Msa0773850-mRNA-1:exon:14752;Parent=Msa0773850-mRNA-1
chr5_2 exon 49210679 49210957 49210679 ID=Msa0773850-mRNA-1:exon:14753;Parent=Msa0773850-mRNA-1
chr5_2 five_prime_UTR 49210224 49210413 49210224 ID=Msa0773850-mRNA-1:five_prime_utr;Parent=Msa0773850-mRNA-1
chr5_2 CDS 49210414 49210560 49210414 ID=Msa0773850-mRNA-1:cds;Parent=Msa0773850-mRNA-1
chr5_2 CDS 49210679 49210807 49210679 ID=Msa0773850-mRNA-1:cds;Parent=Msa0773850-mRNA-1
chr5_2 three_prime_UTR 49210808 49210957 49210808 ID=Msa0773850-mRNA-1:three_prime_utr;Parent=Msa0773850-mRNA-1
Gene Sequence

>Msa0773850

ATGGAAGAGATGGGTTCTTCATCTACAACATCTTTGCCAAATCACAACAAAACTTCTTTTGGTACTCGTTTGAAGATCAGTTGCTTATCTTTTGCCGTTTCTATACAAGAAATTTTTAGATATGCAAAAGCCTTCTTTGTGGGTCAGGCAAAGACTATAACTGCAAAAAATGAGAAGGAAGCAAGTGAAGCTGAACTTGAGGCTTCAAAGAAACAAGTTGAGGCTGCTGATGCTGCTGAACATATCAAGAATAGGCTCAATGATCCTCATAGTTAA

Protein sequence

>Msa0773850

MEEMGSSSTTSLPNHNKTSFGTRLKISCLSFAVSIQEIFRYAKAFFVGQAKTITAKNEKEASEAELEASKKQVEAADAAEHIKNRLNDPHS*