AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1126100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126100 MtrunA17_Chr7g0266721 98.780 164 2 0 1 164 1 164 1.55e-121 339
Msa1126100 MtrunA17_Chr8g0351571 50.331 151 75 0 12 162 3 153 2.26e-53 166
Msa1126100 MtrunA17_Chr2g0321111 52.597 154 72 1 10 162 461 614 1.75e-52 176
Msa1126100 MtrunA17_Chr5g0401141 52.055 146 70 0 12 157 347 492 1.46e-49 167
Msa1126100 MtrunA17_Chr2g0288371 53.247 154 70 2 11 162 14 167 5.05e-43 149
Msa1126100 MtrunA17_Chr1g0193711 50.336 149 68 5 17 160 97 244 4.46e-40 135
Msa1126100 MtrunA17_Chr1g0213971 49.306 144 68 4 18 157 93 235 1.02e-39 134
Msa1126100 MtrunA17_Chr7g0270991 50.000 146 68 4 17 158 108 252 2.18e-39 134
Msa1126100 MtrunA17_Chr4g0045321 48.684 152 65 4 19 158 18 168 4.13e-38 133
Msa1126100 MtrunA17_Chr6g0484281 42.138 159 84 2 12 162 3 161 5.09e-37 134
Msa1126100 MtrunA17_Chr8g0353571 47.552 143 64 4 19 151 32 173 5.65e-37 125
Msa1126100 MtrunA17_Chr7g0274841 47.973 148 65 4 19 155 18 164 9.14e-37 124
Msa1126100 MtrunA17_Chr8g0374621 44.242 165 74 5 10 157 4 167 1.32e-36 129
Msa1126100 MtrunA17_Chr8g0357591 41.509 159 85 2 12 162 3 161 1.30e-35 130
Msa1126100 MtrunA17_Chr3g0145501 51.079 139 52 5 18 143 25 160 4.71e-35 120
Msa1126100 MtrunA17_Chr4g0038431 48.684 152 57 6 10 143 4 152 9.09e-35 119
Msa1126100 MtrunA17_Chr3g0104751 49.624 133 55 4 19 140 48 179 1.07e-34 120
Msa1126100 MtrunA17_Chr7g0260441 47.297 148 64 5 23 158 25 170 5.47e-34 125
Msa1126100 MtrunA17_Chr3g0113441 48.889 135 57 4 17 140 76 209 3.94e-33 117
Msa1126100 MtrunA17_Chr4g0037281 40.945 127 63 3 22 137 64 189 2.61e-26 99.4
Msa1126100 MtrunA17_Chr1g0199071 33.113 151 95 2 13 163 83 227 2.38e-23 92.0
Msa1126100 MtrunA17_Chr3g0138061 38.286 175 89 6 1 158 1 173 5.14e-21 89.0
Msa1126100 MtrunA17_Chr1g0163031 30.263 152 99 3 13 163 82 227 1.09e-19 82.4
Msa1126100 MtrunA17_Chr1g0149061 42.424 132 62 4 24 143 26 155 4.55e-19 83.2
Msa1126100 MtrunA17_Chr3g0122871 46.875 64 34 0 88 151 56 119 1.36e-13 63.5
Msa1126100 MtrunA17_Chr5g0416441 31.544 149 84 5 8 145 24 165 9.38e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126100 AT2G36130.1 86.585 164 22 0 1 164 1 164 3.06e-106 301
Msa1126100 AT1G01940.1 50.331 151 75 0 12 162 3 153 3.80e-53 166
Msa1126100 AT3G44600.1 52.500 160 75 1 4 162 469 628 7.84e-53 177
Msa1126100 AT5G67530.1 49.669 151 76 0 12 162 346 496 1.99e-49 167
Msa1126100 AT4G33060.1 50.968 155 73 3 11 162 14 168 4.50e-44 152
Msa1126100 AT3G62030.3 51.748 143 64 3 19 157 108 249 1.97e-38 132
Msa1126100 AT3G62030.1 51.748 143 64 3 19 157 109 250 2.06e-38 132
Msa1126100 AT1G53720.2 40.994 161 87 3 12 164 3 163 6.50e-38 133
Msa1126100 AT3G62030.2 51.748 143 64 3 19 157 162 303 8.02e-38 132
Msa1126100 AT5G13120.2 48.252 143 69 4 19 157 103 244 1.32e-37 129
Msa1126100 AT5G13120.1 48.252 143 69 4 19 157 107 248 1.61e-37 129
Msa1126100 AT2G21130.1 46.914 162 66 6 7 157 14 166 8.75e-37 125
Msa1126100 AT4G38740.1 48.000 150 65 5 19 157 18 165 1.83e-36 124
Msa1126100 AT3G56070.1 50.360 139 57 3 19 146 18 155 2.68e-36 124
Msa1126100 AT3G56070.2 50.360 139 57 3 19 146 18 155 2.68e-36 124
Msa1126100 AT1G53720.1 40.994 161 87 3 12 164 3 163 2.80e-36 131
Msa1126100 AT2G15790.1 47.020 151 67 4 19 157 18 167 4.64e-36 128
Msa1126100 AT3G63400.2 45.181 166 72 5 10 158 7 170 1.54e-35 127
Msa1126100 AT2G29960.1 48.889 135 57 4 17 140 44 177 3.86e-35 122
Msa1126100 AT2G29960.3 48.889 135 57 4 17 140 44 177 3.86e-35 122
Msa1126100 AT4G34870.1 47.586 145 60 4 16 147 15 156 4.83e-35 120
Msa1126100 AT2G38730.1 46.012 163 72 6 10 157 32 193 6.02e-35 121
Msa1126100 AT2G16600.1 48.344 151 60 6 17 152 17 164 7.67e-35 120
Msa1126100 AT3G55920.1 44.444 153 72 4 19 160 73 223 2.36e-34 120
Msa1126100 AT3G63400.3 45.181 166 72 5 10 158 7 170 3.13e-34 126
Msa1126100 AT3G63400.4 45.181 166 72 5 10 158 7 170 3.13e-34 126
Msa1126100 AT3G63400.1 45.181 166 72 5 10 158 7 170 3.13e-34 126
Msa1126100 AT5G58710.1 47.407 135 59 3 17 140 47 180 4.84e-33 116
Msa1126100 AT2G29960.2 44.444 135 53 5 17 140 44 167 1.23e-27 102
Msa1126100 AT2G16600.2 45.070 142 56 5 17 152 17 142 5.11e-27 99.8
Msa1126100 AT4G34960.1 40.310 129 65 4 18 135 60 187 3.84e-25 96.7
Msa1126100 AT2G47320.1 30.000 150 101 2 13 162 81 226 3.13e-19 81.3
Msa1126100 AT4G34960.2 41.121 107 51 3 18 113 60 165 4.03e-19 79.7
Msa1126100 AT4G32420.1 35.465 172 92 6 5 159 2 171 2.73e-18 81.3
Msa1126100 AT4G32420.5 35.465 172 92 6 5 159 2 171 2.73e-18 81.3
Msa1126100 AT4G32420.3 35.465 172 92 6 5 159 2 171 2.73e-18 81.3
Msa1126100 AT4G32420.6 35.465 172 92 6 5 159 2 171 2.73e-18 81.3
Msa1126100 AT4G32420.4 34.911 169 91 6 5 156 2 168 1.96e-16 76.3
Msa1126100 AT3G66654.4 28.261 138 98 1 14 151 85 221 5.23e-16 73.2
Msa1126100 AT3G66654.2 28.261 138 98 1 14 151 85 221 5.23e-16 73.2
Msa1126100 AT3G66654.3 28.261 138 98 1 14 151 85 221 5.23e-16 73.2
Msa1126100 AT3G66654.1 28.261 138 98 1 14 151 85 221 5.23e-16 73.2
Msa1126100 AT3G66654.5 28.261 138 98 1 14 151 85 221 5.23e-16 73.2
Msa1126100 AT1G53780.2 41.667 84 48 1 80 162 4 87 1.06e-15 73.9
Msa1126100 AT1G26940.1 30.952 168 105 5 6 164 28 193 4.75e-13 64.7
Msa1126100 AT1G26940.2 30.247 162 102 5 12 164 3 162 9.31e-12 60.8

Find 69 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAAGACATAAATTTATT+TGG 0.107913 7_3:+77568214 None:intergenic
AACTGACTTCCGTTAGTATT+AGG 0.209355 7_3:+77570219 None:intergenic
TCTCGCACCGTGCCCCTCTT+TGG 0.259093 7_3:-77570187 Msa1126100:CDS
ATGAAGTTCCTACAAGTTCT+AGG 0.269440 7_3:+77570888 None:intergenic
ACTGACTTCCGTTAGTATTA+GGG 0.308819 7_3:+77570220 None:intergenic
ACCTGTCATTGTTATCTGTT+TGG 0.338632 7_3:+77569305 None:intergenic
ATGTTTATGACCTATCGTTT+TGG 0.344336 7_3:-77568254 Msa1126100:three_prime_UTR
AGAGGAGGAGAATCCATCTA+TGG 0.352009 7_3:-77570678 Msa1126100:CDS
TAGTTCGAACATTGACATAT+TGG 0.365544 7_3:+77568051 None:intergenic
CCAGGAAAACACACAATATT+CGG 0.372270 7_3:-77569373 Msa1126100:intron
ATGCACCACCACCGCTCTAA+TGG 0.390345 7_3:-77568122 Msa1126100:three_prime_UTR
AGTTAAAGCATACCCATAGA+TGG 0.430770 7_3:+77570665 None:intergenic
ACATAAATTTATTTGGAACT+TGG 0.432020 7_3:+77568221 None:intergenic
AATATTCGGAAGAGTAAGTC+GGG 0.456454 7_3:-77569359 Msa1126100:CDS
GGTGGGGATCCCACTGGGAC+AGG 0.457978 7_3:-77570702 Msa1126100:CDS
GATCCCACTGGGACAGGAAG+AGG 0.463282 7_3:-77570696 Msa1126100:CDS
AAGCGCAGAAGGTGGTGCTC+CGG 0.493778 7_3:-77571063 Msa1126100:CDS
TGGTGGTTCGAGATGTTTGT+TGG 0.501946 7_3:+77567982 None:intergenic
CTGTCATGCACGATTGACAT+AGG 0.502222 7_3:-77568182 Msa1126100:three_prime_UTR
CTGCACCATTAGAGCGGTGG+TGG 0.508784 7_3:+77568117 None:intergenic
GAGCTGAAGCACACTGGAGC+TGG 0.513405 7_3:-77570267 Msa1126100:CDS
CCTCCTCTTCCTGTCCCAGT+GGG 0.517919 7_3:+77570693 None:intergenic
GATAAATTACCATCCAAAGA+GGG 0.534312 7_3:+77570174 None:intergenic
GCACCGTGCCCCTCTTTGGA+TGG 0.536332 7_3:-77570183 Msa1126100:intron
CGCAGAAGGTGGTGCTCCGG+AGG 0.537673 7_3:-77571060 Msa1126100:CDS
CGGAAGAGTAAGTCGGGGAA+TGG 0.538120 7_3:-77569353 Msa1126100:CDS
AAAATGTGGGCAAGCGCAGA+AGG 0.539071 7_3:-77571074 Msa1126100:CDS
GAGTAGGATTTCATAGTACA+AGG 0.541774 7_3:-77570723 Msa1126100:intron
ATCGCTGACATAGTTACGAC+TGG 0.547963 7_3:-77568367 Msa1126100:three_prime_UTR
CAAACTGATTACAATTTGAT+CGG 0.548189 7_3:+77568022 None:intergenic
CATATTGGTTCATGCATCAG+AGG 0.556273 7_3:+77568066 None:intergenic
AGGATTTCATAGTACAAGGT+GGG 0.556519 7_3:-77570719 Msa1126100:CDS
TCCGTAGTATCTTCACATCA+TGG 0.557400 7_3:+77568453 None:intergenic
TTGGTTCATGCATCAGAGGA+TGG 0.560824 7_3:+77568070 None:intergenic
TCCAAACAGATAACAATGAC+AGG 0.562883 7_3:-77569306 Msa1126100:intron
TCCTCCTCTTCCTGTCCCAG+TGG 0.564512 7_3:+77570692 None:intergenic
AATAATAATAATTACCTCGA+CGG 0.570411 7_3:+77571007 None:intergenic
TACAAGGTGGGGATCCCACT+GGG 0.581439 7_3:-77570707 Msa1126100:CDS
GGTTACTCTAGAAACTTCCA+TGG 0.582783 7_3:-77571039 Msa1126100:CDS
TAGTATCTTCACATCATGGA+CGG 0.585917 7_3:+77568457 None:intergenic
GTACAAGGTGGGGATCCCAC+TGG 0.586480 7_3:-77570708 Msa1126100:CDS
AGTATCTTCACATCATGGAC+GGG 0.587817 7_3:+77568458 None:intergenic
AGCATGCACCTAGAACTTGT+AGG 0.588442 7_3:-77570896 Msa1126100:CDS
AAACAAGAGCTGAAGCACAC+TGG 0.591406 7_3:-77570273 Msa1126100:CDS
TCCATGATGTGAAGATACTA+CGG 0.592850 7_3:-77568454 Msa1126100:CDS
GAGGAGGAGAATCCATCTAT+GGG 0.597355 7_3:-77570677 Msa1126100:intron
CAATATTCGGAAGAGTAAGT+CGG 0.597820 7_3:-77569360 Msa1126100:CDS
TAAGTTCCACAGAATCATCA+AGG 0.601613 7_3:-77570832 Msa1126100:intron
AGATAAATTACCATCCAAAG+AGG 0.606617 7_3:+77570173 None:intergenic
ATGGAAATCATCAAAAGACT+TGG 0.607942 7_3:-77569334 Msa1126100:CDS
GAAGTTTCTAGAGTAACCTC+CGG 0.610654 7_3:+77571044 None:intergenic
AAGTACAGTTCCAAAACGAT+AGG 0.613096 7_3:+77568244 None:intergenic
CCCACTGGGACAGGAAGAGG+AGG 0.622286 7_3:-77570693 Msa1126100:CDS
CTCGACGGTGAATGAACCCA+TGG 0.626061 7_3:+77571022 None:intergenic
GGATTTCATAGTACAAGGTG+GGG 0.626995 7_3:-77570718 Msa1126100:CDS
TTCATTGAACTATCTCGTAG+AGG 0.631851 7_3:-77570870 Msa1126100:CDS
TGAATACCTTGATGATTCTG+TGG 0.644502 7_3:+77570826 None:intergenic
AATGCTGGCCCTAATACTAA+CGG 0.646655 7_3:-77570228 Msa1126100:CDS
TAGGATTTCATAGTACAAGG+TGG 0.656984 7_3:-77570720 Msa1126100:CDS
GATACTACGGACATCAGTCA+AGG 0.657156 7_3:-77568441 Msa1126100:CDS
ATGTGGGCAAGCGCAGAAGG+TGG 0.657684 7_3:-77571071 Msa1126100:CDS
CATGGGTTCATTCACCGTCG+AGG 0.657749 7_3:-77571021 Msa1126100:intron
GTTACTCTAGAAACTTCCAT+GGG 0.658204 7_3:-77571038 Msa1126100:CDS
TTACCATCCAAAGAGGGGCA+CGG 0.666255 7_3:+77570180 None:intergenic
ATAAATTACCATCCAAAGAG+GGG 0.671498 7_3:+77570175 None:intergenic
TGAAGTCTGCACCATTAGAG+CGG 0.675093 7_3:+77568111 None:intergenic
AAAACGATAGGTCATAAACA+TGG 0.677534 7_3:+77568256 None:intergenic
AGTCTGCACCATTAGAGCGG+TGG 0.686654 7_3:+77568114 None:intergenic
ATATTCGGAAGAGTAAGTCG+GGG 0.743686 7_3:-77569358 Msa1126100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTCTTAAAAATTAGA+TGG - chr7_3:77568339-77568358 Msa1126100:three_prime_UTR 15.0%
!! TTATATATAGTATCTGTAAA+TGG + chr7_3:77569861-77569880 None:intergenic 15.0%
!!! GGTCTAAAATTTAAAAATAT+AGG - chr7_3:77570084-77570103 Msa1126100:intron 15.0%
!!! TTTTAACTATCATGTTATAA+GGG - chr7_3:77570475-77570494 Msa1126100:intron 15.0%
!! AATAATAATAATTACCTCGA+CGG + chr7_3:77568114-77568133 None:intergenic 20.0%
!! TATGTTAATTGTGTTTGTAA+TGG - chr7_3:77568485-77568504 Msa1126100:intron 20.0%
!!! TAATGGTGTTATCATAATAT+GGG - chr7_3:77568502-77568521 Msa1126100:intron 20.0%
!! AAATGAAGTTGTTTATTCAA+AGG - chr7_3:77568732-77568751 Msa1126100:intron 20.0%
!! AATGAAGTTGTTTATTCAAA+GGG - chr7_3:77568733-77568752 Msa1126100:intron 20.0%
!! AATTTCCAAAGTAAAATAGA+AGG + chr7_3:77568978-77568997 None:intergenic 20.0%
!! TGGAAATTATTAATACATGT+TGG - chr7_3:77568990-77569009 Msa1126100:intron 20.0%
!! TTAAATAAGCTCTACTTATA+TGG + chr7_3:77569085-77569104 None:intergenic 20.0%
!! AAAAATGTAAAGATAAAACG+AGG + chr7_3:77569226-77569245 None:intergenic 20.0%
!! ATGATTAAATAGCAATATAG+AGG + chr7_3:77569312-77569331 None:intergenic 20.0%
!! AATAAACATATATGCCAAAA+CGG - chr7_3:77569475-77569494 Msa1126100:intron 20.0%
!!! AATTCATATAGTGAAACTTT+TGG - chr7_3:77569691-77569710 Msa1126100:intron 20.0%
!! ATCATAAAATTGCTAGTATT+GGG - chr7_3:77569930-77569949 Msa1126100:intron 20.0%
!!! TATATGATACTGATAATGTT+GGG - chr7_3:77569953-77569972 Msa1126100:intron 20.0%
!! TTTAAAAATATAGGATCAGA+AGG - chr7_3:77570093-77570112 Msa1126100:intron 20.0%
!! TACATTTAAATAAAACACGT+TGG - chr7_3:77570143-77570162 Msa1126100:intron 20.0%
!! TTAAAACTAGCGTATAATAA+GGG + chr7_3:77570461-77570480 None:intergenic 20.0%
!!! GTTTTAACTATCATGTTATA+AGG - chr7_3:77570474-77570493 Msa1126100:intron 20.0%
!!! CAAATATGTCAATTTTTTCA+AGG + chr7_3:77570619-77570638 None:intergenic 20.0%
!!! AGCATAAAAATTGTAACTTT+TGG - chr7_3:77570807-77570826 Msa1126100:intron 20.0%
!!! ACATAAATTTATTTGGAACT+TGG + chr7_3:77570900-77570919 None:intergenic 20.0%
!! CTGAAAGACATAAATTTATT+TGG + chr7_3:77570907-77570926 None:intergenic 20.0%
! AATAACGAACAATGAAAATG+TGG + chr7_3:77568322-77568341 None:intergenic 25.0%
!!! GTAATGGTGTTATCATAATA+TGG - chr7_3:77568501-77568520 Msa1126100:intron 25.0%
!! ACATGTTCATAAACTGTTTT+CGG - chr7_3:77568586-77568605 Msa1126100:intron 25.0%
! ATGAAGTTGTTTATTCAAAG+GGG - chr7_3:77568734-77568753 Msa1126100:intron 25.0%
!! TTCTTCCTTCTATTTTACTT+TGG - chr7_3:77568970-77568989 Msa1126100:intron 25.0%
!!! TAGATGATTTGGTGATTATA+TGG + chr7_3:77569282-77569301 None:intergenic 25.0%
!!! GAGGATTTTTTTAGATGATT+TGG + chr7_3:77569293-77569312 None:intergenic 25.0%
! TCATTATGTAATGTAATCTG+AGG - chr7_3:77569328-77569347 Msa1126100:CDS 25.0%
! TGCTTATAGTAATATGAATG+TGG - chr7_3:77569382-77569401 Msa1126100:intron 25.0%
! TTGTAGTAAGTAACAATTGT+AGG + chr7_3:77569607-77569626 None:intergenic 25.0%
!!! AAGTGTCATTAAACTGTTTT+AGG + chr7_3:77569897-77569916 None:intergenic 25.0%
! TTAATGACACTTTGTGATAT+TGG - chr7_3:77569905-77569924 Msa1126100:intron 25.0%
! TAATGACACTTTGTGATATT+GGG - chr7_3:77569906-77569925 Msa1126100:intron 25.0%
! GATCATAAAATTGCTAGTAT+TGG - chr7_3:77569929-77569948 Msa1126100:intron 25.0%
!! GTATATGATACTGATAATGT+TGG - chr7_3:77569952-77569971 Msa1126100:intron 25.0%
!! TCAATTACAAGCAAAGTTTT+TGG - chr7_3:77570039-77570058 Msa1126100:intron 25.0%
!! CAATTACAAGCAAAGTTTTT+GGG - chr7_3:77570040-77570059 Msa1126100:intron 25.0%
! ATGTAAATTGAATATGCACA+AGG + chr7_3:77570128-77570147 None:intergenic 25.0%
!!! AACTACTTTTTGTTCCATTT+TGG + chr7_3:77570337-77570356 None:intergenic 25.0%
! AATGGAACAAAAAGTAGTTA+TGG - chr7_3:77570338-77570357 Msa1126100:intron 25.0%
! ATGGAACAAAAAGTAGTTAT+GGG - chr7_3:77570339-77570358 Msa1126100:intron 25.0%
! GTTAAAACTAGCGTATAATA+AGG + chr7_3:77570462-77570481 None:intergenic 25.0%
! TTTCATAAAATTCCATTTCC+TGG + chr7_3:77570507-77570526 None:intergenic 25.0%
! CCAAGAAAAAAAGTTCATAA+AGG + chr7_3:77570577-77570596 None:intergenic 25.0%
!!! CCTTTATGAACTTTTTTTCT+TGG - chr7_3:77570574-77570593 Msa1126100:intron 25.0%
!!! TGTTCTATTTCTACTTATTC+AGG - chr7_3:77570637-77570656 Msa1126100:intron 25.0%
! CAAACTGATTACAATTTGAT+CGG + chr7_3:77571099-77571118 None:intergenic 25.0%
!!! TGTGTTTTGAGAGAAAAATG+TGG - chr7_3:77568030-77568049 Msa1126100:three_prime_UTR 30.0%
!!! GTGTTTTGAGAGAAAAATGT+GGG - chr7_3:77568031-77568050 Msa1126100:three_prime_UTR 30.0%
TTAAACCCTGATACAGAATT+AGG - chr7_3:77568155-77568174 Msa1126100:three_prime_UTR 30.0%
TAAACCCTGATACAGAATTA+GGG - chr7_3:77568156-77568175 Msa1126100:three_prime_UTR 30.0%
AAAACCCTAATTCTGTATCA+GGG + chr7_3:77568163-77568182 None:intergenic 30.0%
TAAAACCCTAATTCTGTATC+AGG + chr7_3:77568164-77568183 None:intergenic 30.0%
!! GTTCTTTACATTGGTTTATG+TGG - chr7_3:77568366-77568385 Msa1126100:three_prime_UTR 30.0%
TATCATAATATGGGTCTGTT+TGG - chr7_3:77568511-77568530 Msa1126100:intron 30.0%
ATCAACTGACATAAACTGTT+TGG - chr7_3:77568552-77568571 Msa1126100:intron 30.0%
TCAACTGACATAAACTGTTT+GGG - chr7_3:77568553-77568572 Msa1126100:intron 30.0%
! CATTTTTGTATAAGCTGTCT+TGG + chr7_3:77568630-77568649 None:intergenic 30.0%
TGAAGTTGTTTATTCAAAGG+GGG - chr7_3:77568735-77568754 Msa1126100:intron 30.0%
!! TTTAGCCACTTTGTTGTTTA+TGG - chr7_3:77568797-77568816 Msa1126100:intron 30.0%
! ATTTTATCAATGGCTAATGC+TGG - chr7_3:77568875-77568894 Msa1126100:intron 30.0%
ATAAATTACCATCCAAAGAG+GGG + chr7_3:77568946-77568965 None:intergenic 30.0%
GATAAATTACCATCCAAAGA+GGG + chr7_3:77568947-77568966 None:intergenic 30.0%
AGATAAATTACCATCCAAAG+AGG + chr7_3:77568948-77568967 None:intergenic 30.0%
CCAAACAAAAATCATCAACA+AGG + chr7_3:77569017-77569036 None:intergenic 30.0%
!!! CCTTGTTGATGATTTTTGTT+TGG - chr7_3:77569014-77569033 Msa1126100:intron 30.0%
CCAAAAACAAACCAATAGTT+GGG + chr7_3:77569120-77569139 None:intergenic 30.0%
TCCAAAAACAAACCAATAGT+TGG + chr7_3:77569121-77569140 None:intergenic 30.0%
AAATTTGAACCCAATAAGCA+TGG + chr7_3:77569151-77569170 None:intergenic 30.0%
! GTTTCATTGACTAAATCTGT+AGG - chr7_3:77569526-77569545 Msa1126100:intron 30.0%
TGTAGGAATGCAAAAAACAA+GGG - chr7_3:77569543-77569562 Msa1126100:intron 30.0%
ATCATCTGATGCTAATGTTA+TGG + chr7_3:77569661-77569680 None:intergenic 30.0%
ATGGAAATCATCAAAAGACT+TGG - chr7_3:77569784-77569803 Msa1126100:intron 30.0%
AATGACACTTTGTGATATTG+GGG - chr7_3:77569907-77569926 Msa1126100:intron 30.0%
AAATAAAACACGTTGGATCT+AGG - chr7_3:77570150-77570169 Msa1126100:intron 30.0%
ATCTAGGTTTGGATATATAG+TGG - chr7_3:77570166-77570185 Msa1126100:intron 30.0%
TTGGATATATAGTGGCTAAT+TGG - chr7_3:77570174-77570193 Msa1126100:intron 30.0%
TGGATATATAGTGGCTAATT+GGG - chr7_3:77570175-77570194 Msa1126100:intron 30.0%
! AAAACCGAGATTTTGTGTAA+AGG - chr7_3:77570258-77570277 Msa1126100:CDS 30.0%
GATGCCACAAAATAAGTTAA+AGG - chr7_3:77570370-77570389 Msa1126100:intron 30.0%
!!! TGGTCCTTTAACTTATTTTG+TGG + chr7_3:77570377-77570396 None:intergenic 30.0%
AAAGGACCAAATGTATGATT+TGG - chr7_3:77570388-77570407 Msa1126100:intron 30.0%
AAGGACCAAATGTATGATTT+GGG - chr7_3:77570389-77570408 Msa1126100:intron 30.0%
GAAAAATGTCATGGCATATT+TGG - chr7_3:77570773-77570792 Msa1126100:intron 30.0%
!!! GGTTTTTAGTTTTCATTGTG+TGG + chr7_3:77570844-77570863 None:intergenic 30.0%
AAAACGATAGGTCATAAACA+TGG + chr7_3:77570865-77570884 None:intergenic 30.0%
! ATGTTTATGACCTATCGTTT+TGG - chr7_3:77570864-77570883 Msa1126100:CDS 30.0%
TAGTTCGAACATTGACATAT+TGG + chr7_3:77571070-77571089 None:intergenic 30.0%
! GTTACTCTAGAAACTTCCAT+GGG - chr7_3:77568080-77568099 Msa1126100:three_prime_UTR 35.0%
ATGAAGTTCCTACAAGTTCT+AGG + chr7_3:77568233-77568252 None:intergenic 35.0%
TTCATTGAACTATCTCGTAG+AGG - chr7_3:77568248-77568267 Msa1126100:three_prime_UTR 35.0%
TAAGTTCCACAGAATCATCA+AGG - chr7_3:77568286-77568305 Msa1126100:three_prime_UTR 35.0%
! TGAATACCTTGATGATTCTG+TGG + chr7_3:77568295-77568314 None:intergenic 35.0%
!! GATGGTGTTGTTCTTTACAT+TGG - chr7_3:77568357-77568376 Msa1126100:three_prime_UTR 35.0%
!! GTTTATGTGGTTTGTTGAGT+AGG - chr7_3:77568379-77568398 Msa1126100:three_prime_UTR 35.0%
GAGTAGGATTTCATAGTACA+AGG - chr7_3:77568395-77568414 Msa1126100:three_prime_UTR 35.0%
TAGGATTTCATAGTACAAGG+TGG - chr7_3:77568398-77568417 Msa1126100:three_prime_UTR 35.0%
AGGATTTCATAGTACAAGGT+GGG - chr7_3:77568399-77568418 Msa1126100:three_prime_UTR 35.0%
AGTTAAAGCATACCCATAGA+TGG + chr7_3:77568456-77568475 None:intergenic 35.0%
TAATATGGGTCTGTTTGGAT+CGG - chr7_3:77568516-77568535 Msa1126100:intron 35.0%
CAACTGACATAAACTGTTTG+GGG - chr7_3:77568554-77568573 Msa1126100:intron 35.0%
AACTGACATAAACTGTTTGG+GGG - chr7_3:77568555-77568574 Msa1126100:intron 35.0%
TAAGTCACCCAAAAAAACCT+AGG + chr7_3:77568761-77568780 None:intergenic 35.0%
! TGGAGCTGGTATTTTATCAA+TGG - chr7_3:77568865-77568884 Msa1126100:intron 35.0%
! ACTGACTTCCGTTAGTATTA+GGG + chr7_3:77568901-77568920 None:intergenic 35.0%
! AACTGACTTCCGTTAGTATT+AGG + chr7_3:77568902-77568921 None:intergenic 35.0%
!! TGATAAGAGTACCCAACTAT+TGG - chr7_3:77569106-77569125 Msa1126100:intron 35.0%
!!! CCCAACTATTGGTTTGTTTT+TGG - chr7_3:77569117-77569136 Msa1126100:intron 35.0%
GGCAAACACAATAGATAATC+AGG - chr7_3:77569359-77569378 Msa1126100:CDS 35.0%
TGAATGTGGATGATTATCTC+TGG - chr7_3:77569396-77569415 Msa1126100:intron 35.0%
GAATGTGGATGATTATCTCT+GGG - chr7_3:77569397-77569416 Msa1126100:intron 35.0%
CTGTAGGAATGCAAAAAACA+AGG - chr7_3:77569542-77569561 Msa1126100:intron 35.0%
ACTCAATTTCTGTTTCAACC+AGG - chr7_3:77569727-77569746 Msa1126100:intron 35.0%
CCAGGAAAACACACAATATT+CGG - chr7_3:77569745-77569764 Msa1126100:intron 35.0%
CAATATTCGGAAGAGTAAGT+CGG - chr7_3:77569758-77569777 Msa1126100:intron 35.0%
AATATTCGGAAGAGTAAGTC+GGG - chr7_3:77569759-77569778 Msa1126100:intron 35.0%
TCCAAACAGATAACAATGAC+AGG - chr7_3:77569812-77569831 Msa1126100:intron 35.0%
ACCTGTCATTGTTATCTGTT+TGG + chr7_3:77569816-77569835 None:intergenic 35.0%
!! ACTGTTTTAGGCAAGATTGT+TGG + chr7_3:77569885-77569904 None:intergenic 35.0%
GAATATTGTAACAGTGCATG+AGG + chr7_3:77570021-77570040 None:intergenic 35.0%
! AATTTTAGACCTCGACAGAT+AGG + chr7_3:77570075-77570094 None:intergenic 35.0%
!! AGTAGTTGATTTGGGTTAGA+AGG - chr7_3:77570233-77570252 Msa1126100:CDS 35.0%
!! GTAGTTGATTTGGGTTAGAA+GGG - chr7_3:77570234-77570253 Msa1126100:CDS 35.0%
!! TAGTTGATTTGGGTTAGAAG+GGG - chr7_3:77570235-77570254 Msa1126100:CDS 35.0%
! GTAAAGTTAGGGTACCAAAA+TGG - chr7_3:77570320-77570339 Msa1126100:intron 35.0%
!!! CTTATTTTGTGGCATCACTT+TGG + chr7_3:77570366-77570385 None:intergenic 35.0%
CAAAGCCCAAATCATACATT+TGG + chr7_3:77570397-77570416 None:intergenic 35.0%
! AATAAGGGTGGTAAAGTGTT+GGG + chr7_3:77570446-77570465 None:intergenic 35.0%
TAATAAGGGTGGTAAAGTGT+TGG + chr7_3:77570447-77570466 None:intergenic 35.0%
AAACTAGCGTATAATAAGGG+TGG + chr7_3:77570458-77570477 None:intergenic 35.0%
TAGTATCTTCACATCATGGA+CGG + chr7_3:77570664-77570683 None:intergenic 35.0%
TCCATGATGTGAAGATACTA+CGG - chr7_3:77570664-77570683 Msa1126100:intron 35.0%
TTACGACTGGAAAAATGTCA+TGG - chr7_3:77570764-77570783 Msa1126100:intron 35.0%
! TGTAACTTTTGGCACTAGAT+TGG - chr7_3:77570818-77570837 Msa1126100:intron 35.0%
AAGTACAGTTCCAAAACGAT+AGG + chr7_3:77570877-77570896 None:intergenic 35.0%
!! GAAGTTTCTAGAGTAACCTC+CGG + chr7_3:77568077-77568096 None:intergenic 40.0%
! GGTTACTCTAGAAACTTCCA+TGG - chr7_3:77568079-77568098 Msa1126100:three_prime_UTR 40.0%
GGATTTCATAGTACAAGGTG+GGG - chr7_3:77568400-77568419 Msa1126100:three_prime_UTR 40.0%
CACTGCCATAAACAACAAAG+TGG + chr7_3:77568805-77568824 None:intergenic 40.0%
AATGCTGGCCCTAATACTAA+CGG - chr7_3:77568890-77568909 Msa1126100:intron 40.0%
GACAATGCACCATGCTTATT+GGG - chr7_3:77569139-77569158 Msa1126100:intron 40.0%
! ATGTAATCTGAGGTCACTGA+TGG - chr7_3:77569338-77569357 Msa1126100:CDS 40.0%
! CAGCTGTCTAATATCCGTTT+TGG + chr7_3:77569492-77569511 None:intergenic 40.0%
! CCGAATATTGTGTGTTTTCC+TGG + chr7_3:77569748-77569767 None:intergenic 40.0%
ATATTCGGAAGAGTAAGTCG+GGG - chr7_3:77569760-77569779 Msa1126100:intron 40.0%
! TCAGATGACCAACATTTTCG+AGG + chr7_3:77569982-77570001 None:intergenic 40.0%
TCGAAAATGTTGGTCATCTG+AGG - chr7_3:77569981-77570000 Msa1126100:intron 40.0%
AAACACGTTGGATCTAGGTT+TGG - chr7_3:77570155-77570174 Msa1126100:intron 40.0%
GTCGCCTTTACACAAAATCT+CGG + chr7_3:77570265-77570284 None:intergenic 40.0%
TTTGTGTAAAGGCGACAGAT+TGG - chr7_3:77570269-77570288 Msa1126100:CDS 40.0%
TGACCAACTGAGGTAAAGTT+AGG - chr7_3:77570308-77570327 Msa1126100:intron 40.0%
GACCAACTGAGGTAAAGTTA+GGG - chr7_3:77570309-77570328 Msa1126100:intron 40.0%
TACCCTAACTTTACCTCAGT+TGG + chr7_3:77570314-77570333 None:intergenic 40.0%
!! ATGTTATAAGGGTGTCTTCC+AGG - chr7_3:77570486-77570505 Msa1126100:intron 40.0%
AGTATCTTCACATCATGGAC+GGG + chr7_3:77570663-77570682 None:intergenic 40.0%
TCCGTAGTATCTTCACATCA+TGG + chr7_3:77570668-77570687 None:intergenic 40.0%
!! CATATTGGTTCATGCATCAG+AGG + chr7_3:77571055-77571074 None:intergenic 40.0%
AGCATGCACCTAGAACTTGT+AGG - chr7_3:77568222-77568241 Msa1126100:three_prime_UTR 45.0%
AGAGGAGGAGAATCCATCTA+TGG - chr7_3:77568440-77568459 Msa1126100:CDS 45.0%
GAGGAGGAGAATCCATCTAT+GGG - chr7_3:77568441-77568460 Msa1126100:CDS 45.0%
TGTTTATTCAAAGGGGGCCT+AGG - chr7_3:77568741-77568760 Msa1126100:intron 45.0%
!!! AAAGGGGGCCTAGGTTTTTT+TGG - chr7_3:77568750-77568769 Msa1126100:intron 45.0%
!!! AAGGGGGCCTAGGTTTTTTT+GGG - chr7_3:77568751-77568770 Msa1126100:intron 45.0%
AAACAAGAGCTGAAGCACAC+TGG - chr7_3:77568845-77568864 Msa1126100:intron 45.0%
CACCATGCAAGTGAGTAACA+CGG + chr7_3:77569042-77569061 None:intergenic 45.0%
TGTTACTCACTTGCATGGTG+AGG - chr7_3:77569042-77569061 Msa1126100:intron 45.0%
GGACAATGCACCATGCTTAT+TGG - chr7_3:77569138-77569157 Msa1126100:intron 45.0%
TTGGGTGTCCTCGAAAATGT+TGG - chr7_3:77569971-77569990 Msa1126100:intron 45.0%
TCCACGCTGAGTAGTTGATT+TGG - chr7_3:77570224-77570243 Msa1126100:CDS 45.0%
! CCACGCTGAGTAGTTGATTT+GGG - chr7_3:77570225-77570244 Msa1126100:CDS 45.0%
TGTAAAGGCGACAGATTGGA+CGG - chr7_3:77570273-77570292 Msa1126100:CDS 45.0%
!! TAAGGGTGTCTTCCAGGAAA+TGG - chr7_3:77570492-77570511 Msa1126100:intron 45.0%
GATACTACGGACATCAGTCA+AGG - chr7_3:77570677-77570696 Msa1126100:intron 45.0%
ATCGCTGACATAGTTACGAC+TGG - chr7_3:77570751-77570770 Msa1126100:intron 45.0%
CTGTCATGCACGATTGACAT+AGG - chr7_3:77570936-77570955 Msa1126100:intron 45.0%
TGAAGTCTGCACCATTAGAG+CGG + chr7_3:77571010-77571029 None:intergenic 45.0%
TTGGTTCATGCATCAGAGGA+TGG + chr7_3:77571051-77571070 None:intergenic 45.0%
!!! TTAACATTTTTTTTTTTTTT+TGG - chr7_3:77568181-77568200 Msa1126100:three_prime_UTR 5.0%
!!! TAACATTTTTTTTTTTTTTT+GGG - chr7_3:77568182-77568201 Msa1126100:three_prime_UTR 5.0%
GAGAGAGAGAGAGAGAGTGA+AGG - chr7_3:77567994-77568013 Msa1126100:three_prime_UTR 50.0%
AAAATGTGGGCAAGCGCAGA+AGG - chr7_3:77568044-77568063 Msa1126100:three_prime_UTR 50.0%
TTACCATCCAAAGAGGGGCA+CGG + chr7_3:77568941-77568960 None:intergenic 50.0%
! CACCGTGTTACTCACTTGCA+TGG - chr7_3:77569037-77569056 Msa1126100:intron 50.0%
CAACGAAAGCCTATCTGTCG+AGG - chr7_3:77570063-77570082 Msa1126100:intron 50.0%
CCCAAATCAACTACTCAGCG+TGG + chr7_3:77570228-77570247 None:intergenic 50.0%
AGGGCTAACTTGACCAACTG+AGG - chr7_3:77570298-77570317 Msa1126100:CDS 50.0%
CTCGACGGTGAATGAACCCA+TGG + chr7_3:77568099-77568118 None:intergenic 55.0%
CATGGGTTCATTCACCGTCG+AGG - chr7_3:77568097-77568116 Msa1126100:three_prime_UTR 55.0%
TACAAGGTGGGGATCCCACT+GGG - chr7_3:77568411-77568430 Msa1126100:three_prime_UTR 55.0%
CGGAAGAGTAAGTCGGGGAA+TGG - chr7_3:77569765-77569784 Msa1126100:intron 55.0%
AGGCGACAGATTGGACGGAA+AGG - chr7_3:77570278-77570297 Msa1126100:CDS 55.0%
GGCGACAGATTGGACGGAAA+GGG - chr7_3:77570279-77570298 Msa1126100:CDS 55.0%
ATGCACCACCACCGCTCTAA+TGG - chr7_3:77570996-77571015 Msa1126100:intron 55.0%
AGTCTGCACCATTAGAGCGG+TGG + chr7_3:77571007-77571026 None:intergenic 55.0%
ATGTGGGCAAGCGCAGAAGG+TGG - chr7_3:77568047-77568066 Msa1126100:three_prime_UTR 60.0%
AAGCGCAGAAGGTGGTGCTC+CGG - chr7_3:77568055-77568074 Msa1126100:three_prime_UTR 60.0%
GTACAAGGTGGGGATCCCAC+TGG - chr7_3:77568410-77568429 Msa1126100:three_prime_UTR 60.0%
GATCCCACTGGGACAGGAAG+AGG - chr7_3:77568422-77568441 Msa1126100:exon 60.0%
CCTCCTCTTCCTGTCCCAGT+GGG + chr7_3:77568428-77568447 None:intergenic 60.0%
TCCTCCTCTTCCTGTCCCAG+TGG + chr7_3:77568429-77568448 None:intergenic 60.0%
GAGCTGAAGCACACTGGAGC+TGG - chr7_3:77568851-77568870 Msa1126100:intron 60.0%
CTGCACCATTAGAGCGGTGG+TGG + chr7_3:77571004-77571023 None:intergenic 60.0%
CCCACTGGGACAGGAAGAGG+AGG - chr7_3:77568425-77568444 Msa1126100:exon 65.0%
TCTCGCACCGTGCCCCTCTT+TGG - chr7_3:77568931-77568950 Msa1126100:intron 65.0%
GCACCGTGCCCCTCTTTGGA+TGG - chr7_3:77568935-77568954 Msa1126100:intron 65.0%
CGCAGAAGGTGGTGCTCCGG+AGG - chr7_3:77568058-77568077 Msa1126100:three_prime_UTR 70.0%
GGTGGGGATCCCACTGGGAC+AGG - chr7_3:77568416-77568435 Msa1126100:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 77567993 77571147 77567993 ID=Msa1126100;Name=Msa1126100
chr7_3 mRNA 77567993 77571147 77567993 ID=Msa1126100-mRNA-1;Parent=Msa1126100;Name=Msa1126100-mRNA-1;_AED=0.09;_eAED=0.09;_QI=54|1|1|1|0.8|1|6|443|164
chr7_3 exon 77567993 77568481 77567993 ID=Msa1126100-mRNA-1:exon:21469;Parent=Msa1126100-mRNA-1
chr7_3 exon 77569307 77569391 77569307 ID=Msa1126100-mRNA-1:exon:21468;Parent=Msa1126100-mRNA-1
chr7_3 exon 77570184 77570317 77570184 ID=Msa1126100-mRNA-1:exon:21467;Parent=Msa1126100-mRNA-1
chr7_3 exon 77570678 77570739 77570678 ID=Msa1126100-mRNA-1:exon:21466;Parent=Msa1126100-mRNA-1
chr7_3 exon 77570833 77570928 77570833 ID=Msa1126100-mRNA-1:exon:21465;Parent=Msa1126100-mRNA-1
chr7_3 exon 77571022 77571147 77571022 ID=Msa1126100-mRNA-1:exon:21464;Parent=Msa1126100-mRNA-1
chr7_3 five_prime_UTR 77571094 77571147 77571094 ID=Msa1126100-mRNA-1:five_prime_utr;Parent=Msa1126100-mRNA-1
chr7_3 CDS 77571022 77571093 77571022 ID=Msa1126100-mRNA-1:cds;Parent=Msa1126100-mRNA-1
chr7_3 CDS 77570833 77570928 77570833 ID=Msa1126100-mRNA-1:cds;Parent=Msa1126100-mRNA-1
chr7_3 CDS 77570678 77570739 77570678 ID=Msa1126100-mRNA-1:cds;Parent=Msa1126100-mRNA-1
chr7_3 CDS 77570184 77570317 77570184 ID=Msa1126100-mRNA-1:cds;Parent=Msa1126100-mRNA-1
chr7_3 CDS 77569307 77569391 77569307 ID=Msa1126100-mRNA-1:cds;Parent=Msa1126100-mRNA-1
chr7_3 CDS 77568436 77568481 77568436 ID=Msa1126100-mRNA-1:cds;Parent=Msa1126100-mRNA-1
chr7_3 three_prime_UTR 77567993 77568435 77567993 ID=Msa1126100-mRNA-1:three_prime_utr;Parent=Msa1126100-mRNA-1
Gene Sequence

>Msa1126100

ATGTGGGCAAGCGCAGAAGGTGGTGCTCCGGAGGTTACTCTAGAAACTTCCATGGGTTCATTCACCGTCGAGTTGTACTACAAGCATGCACCTAGAACTTGTAGGAACTTCATTGAACTATCTCGTAGAGGTTATTACGACAACGTTAAGTTCCACAGAATCATCAAGGATTTCATAGTACAAGGTGGGGATCCCACTGGGACAGGAAGAGGAGGAGAATCCATCTATGGTGCTAAATTTGAAGATGAGATTAAACAAGAGCTGAAGCACACTGGAGCTGGTATTTTATCAATGGCTAATGCTGGCCCTAATACTAACGGAAGTCAGTTCTTTATTACTCTCGCACCGTGCCCCTCTTTGGATGGAAAACACACAATATTCGGAAGAGTAAGTCGGGGAATGGAAATCATCAAAAGACTTGGCAGTGTCCAAACAGATAACAATGACAGGCCCGTCCATGATGTGAAGATACTACGGACATCAGTCAAGGATTGA
Protein sequence

>Msa1126100

MWASAEGGAPEVTLETSMGSFTVELYYKHAPRTCRNFIELSRRGYYDNVKFHRIIKDFIVQGGDPTGTGRGGESIYGAKFEDEIKQELKHTGAGILSMANAGPNTNGSQFFITLAPCPSLDGKHTIFGRVSRGMEIIKRLGSVQTDNNDRPVHDVKILRTSVKD*