AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1252200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1252200 MtrunA17_Chr8g0368161 96.205 448 17 0 1 448 1 448 0.0 886
Msa1252200 MtrunA17_Chr5g0413131 69.488 449 115 7 20 448 17 463 0.0 629
Msa1252200 MtrunA17_Chr4g0058771 61.311 473 149 8 8 448 11 481 0.0 594
Msa1252200 MtrunA17_Chr3g0117271 69.509 387 115 3 63 448 112 496 0.0 577
Msa1252200 MtrunA17_Chr8g0368241 64.734 414 143 1 38 448 1 414 0.0 559
Msa1252200 MtrunA17_Chr2g0314461 67.848 395 118 5 57 448 18 406 0.0 549
Msa1252200 MtrunA17_Chr2g0314451 64.951 408 130 6 48 448 4 405 0.0 532
Msa1252200 MtrunA17_Chr2g0314471 63.772 403 135 6 51 448 10 406 0.0 525
Msa1252200 MtrunA17_Chr7g0274951 58.290 386 137 4 86 447 54 439 2.12e-164 470
Msa1252200 MtrunA17_Chr1g0189961 52.436 431 171 6 51 448 22 451 1.21e-159 458
Msa1252200 MtrunA17_Chr8g0380511 53.774 424 160 6 45 447 7 415 1.40e-159 457
Msa1252200 MtrunA17_Chr8g0368291 54.524 420 167 7 38 448 1 405 3.57e-157 451
Msa1252200 MtrunA17_Chr2g0309251 53.886 386 157 3 83 448 42 426 1.23e-154 445
Msa1252200 MtrunA17_Chr8g0368201 59.938 322 111 4 143 448 1 320 1.15e-135 392
Msa1252200 MtrunA17_Chr8g0368341 55.908 347 137 5 103 448 4 335 9.90e-133 385
Msa1252200 MtrunA17_Chr8g0368221 60.140 286 86 3 38 323 1 258 1.26e-115 339
Msa1252200 MtrunA17_Chr2g0326371 57.321 321 121 8 132 448 52 360 1.70e-115 343
Msa1252200 MtrunA17_Chr8g0368351 59.004 261 88 5 1 257 3 248 1.40e-100 301
Msa1252200 MtrunA17_Chr8g0368181 70.984 193 55 1 256 448 2 193 2.69e-96 287
Msa1252200 MtrunA17_Chr6g0453411 41.005 378 182 10 102 448 8 375 6.44e-91 280
Msa1252200 MtrunA17_Chr3g0135381 38.974 390 211 6 79 443 45 432 3.59e-89 279
Msa1252200 MtrunA17_Chr3g0135371 37.644 433 230 11 40 448 19 435 8.06e-89 277
Msa1252200 MtrunA17_Chr3g0129891 56.769 229 88 4 224 447 157 379 1.50e-81 256
Msa1252200 MtrunA17_Chr3g0129891 60.000 190 68 2 15 201 8 192 4.50e-71 229
Msa1252200 MtrunA17_Chr8g0368331 44.404 277 122 4 201 448 13 286 4.84e-77 241
Msa1252200 MtrunA17_Chr8g0368361 56.410 195 82 2 256 448 2 195 2.01e-76 236
Msa1252200 MtrunA17_Chr8g0368281 57.513 193 81 1 256 447 2 194 3.68e-76 236
Msa1252200 MtrunA17_Chr3g0135401 39.564 321 161 7 150 444 33 346 1.06e-70 227
Msa1252200 MtrunA17_Chr1g0151871 39.130 299 170 5 151 448 40 327 5.86e-69 222
Msa1252200 MtrunA17_Chr8g0368171 50.439 228 81 7 38 257 1 204 2.30e-63 203
Msa1252200 MtrunA17_Chr8g0368231 72.800 125 34 0 324 448 8 132 2.03e-60 193
Msa1252200 MtrunA17_Chr8g0368271 59.477 153 59 1 38 187 1 153 3.54e-56 184
Msa1252200 MtrunA17_Chr8g0368261 63.077 130 46 2 320 448 1 129 8.08e-53 173
Msa1252200 MtrunA17_Chr8g0368321 59.603 151 51 3 38 187 1 142 2.13e-51 171
Msa1252200 MtrunA17_Chr8g0368381 61.607 112 43 0 256 367 2 113 2.13e-46 156
Msa1252200 MtrunA17_Chr3g0135431 40.708 226 99 6 82 278 41 260 4.57e-46 164
Msa1252200 MtrunA17_Chr3g0135441 35.433 254 137 6 198 448 3 232 2.96e-45 157
Msa1252200 MtrunA17_Chr3g0138811 30.226 354 219 13 99 444 53 386 1.87e-41 151
Msa1252200 MtrunA17_Chr2g0302691 30.618 356 210 11 100 442 37 368 2.21e-37 140
Msa1252200 MtrunA17_Chr8g0339871 37.083 240 135 9 213 442 4 237 5.72e-37 135
Msa1252200 MtrunA17_Chr8g0391201 34.711 242 138 9 212 442 3 235 1.53e-32 123
Msa1252200 MtrunA17_Chr5g0445181 28.947 342 176 12 46 383 19 297 9.06e-32 123
Msa1252200 MtrunA17_Chr2g0302651 33.028 218 115 8 234 442 1 196 3.24e-23 97.1
Msa1252200 MtrunA17_Chr3g0135451 42.857 126 51 4 82 187 35 159 8.83e-23 95.5
Msa1252200 MtrunA17_Chr5g0434771 38.889 162 91 6 266 425 9 164 9.06e-22 92.4
Msa1252200 MtrunA17_Chr8g0353941 34.574 188 113 6 261 444 1 182 1.20e-21 92.0
Msa1252200 MtrunA17_Chr8g0353971 35.673 171 102 5 276 442 4 170 1.59e-21 91.3
Msa1252200 MtrunA17_Chr8g0353931 30.415 217 125 7 51 257 3 203 1.99e-21 92.0
Msa1252200 MtrunA17_Chr5g0426971 27.027 259 162 6 186 442 23 256 2.63e-17 81.6
Msa1252200 MtrunA17_Chr8g0368191 52.055 73 31 2 48 117 6 77 1.49e-16 75.9
Msa1252200 MtrunA17_Chr8g0353961 36.893 103 62 2 86 185 33 135 3.74e-15 72.8
Msa1252200 MtrunA17_Chr5g0443801 35.135 111 70 2 211 321 21 129 2.56e-14 71.2
Msa1252200 MtrunA17_Chr3g0135411 57.447 47 20 0 82 128 61 107 3.70e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1252200 AT1G10750.1 73.560 382 100 1 67 448 87 467 0.0 613
Msa1252200 AT1G23340.2 67.990 403 127 2 47 448 8 409 0.0 588
Msa1252200 AT1G23340.1 67.990 403 127 2 47 448 8 409 0.0 588
Msa1252200 AT1G70550.1 70.449 379 111 1 70 448 88 465 0.0 582
Msa1252200 AT5G50150.1 69.895 382 112 3 69 448 40 420 0.0 571
Msa1252200 AT1G55360.1 56.477 386 149 5 81 448 38 422 3.87e-159 456
Msa1252200 AT3G13510.1 53.995 413 159 7 53 448 21 419 6.51e-159 456
Msa1252200 AT5G18460.1 56.771 384 140 5 86 447 50 429 6.69e-158 454
Msa1252200 AT5G56530.2 53.699 419 152 7 51 447 21 419 4.26e-154 444
Msa1252200 AT5G56530.1 53.699 419 152 7 51 447 21 419 4.26e-154 444
Msa1252200 AT2G44210.1 51.196 418 174 7 51 448 8 415 2.33e-148 429
Msa1252200 AT2G44210.2 47.991 448 173 8 51 448 8 445 1.33e-142 415
Msa1252200 AT1G23340.3 66.906 278 90 2 47 323 8 284 4.47e-136 393
Msa1252200 AT2G44220.1 48.058 412 185 9 51 448 7 403 3.47e-133 390
Msa1252200 AT2G44240.1 49.737 380 180 7 77 448 26 402 2.27e-124 367
Msa1252200 AT2G17750.1 42.092 411 211 10 47 447 2 395 8.97e-114 340
Msa1252200 AT3G48230.1 47.733 375 184 8 82 448 3 373 2.01e-111 333
Msa1252200 AT2G44250.1 45.721 409 201 10 51 447 9 408 1.99e-109 329
Msa1252200 AT2G19360.1 42.069 435 207 9 44 447 5 425 2.21e-108 327
Msa1252200 AT5G19170.1 45.787 356 169 7 98 442 23 365 6.20e-104 315
Msa1252200 AT1G70550.2 69.268 205 62 1 70 274 88 291 3.32e-102 306
Msa1252200 AT1G10750.2 60.965 228 87 2 67 293 87 313 8.64e-95 290
Msa1252200 AT2G44250.2 49.186 307 143 7 151 447 22 325 1.86e-88 273
Msa1252200 AT5G25950.1 37.385 436 226 9 43 448 14 432 1.42e-82 261
Msa1252200 AT2G20170.1 38.667 375 204 13 83 442 33 396 1.42e-73 237
Msa1252200 AT2G20170.2 38.667 375 204 13 83 442 33 396 1.42e-73 237
Msa1252200 AT5G25960.1 37.500 408 196 8 49 448 1 357 2.70e-73 234
Msa1252200 AT4G23390.1 37.772 368 203 13 90 442 41 397 1.59e-66 218
Msa1252200 AT4G23370.1 35.279 377 219 12 83 442 33 401 3.14e-66 218
Msa1252200 AT2G20170.3 36.267 375 196 13 83 442 33 379 1.43e-64 213
Msa1252200 AT4G23380.1 33.090 411 242 13 46 442 7 398 5.24e-62 206
Msa1252200 AT4G23350.1 31.989 372 234 9 83 442 36 400 3.74e-59 199
Msa1252200 AT4G23365.1 32.527 372 230 11 83 442 36 398 6.22e-59 199
Msa1252200 AT4G23355.1 32.533 375 226 11 83 442 36 398 3.52e-57 194
Msa1252200 AT4G23360.1 32.891 377 223 12 83 442 36 399 3.19e-56 191
Msa1252200 AT2G35250.1 34.604 341 195 10 107 444 24 339 1.13e-51 177
Msa1252200 AT1G10190.1 30.993 413 242 18 52 442 3 394 5.00e-45 161
Msa1252200 AT4G17505.1 30.704 355 191 13 98 448 18 321 5.86e-45 159
Msa1252200 AT4G15050.1 30.769 377 221 17 86 442 38 394 3.19e-41 151
Msa1252200 AT2G24950.1 29.730 407 245 17 51 442 29 409 1.08e-39 147
Msa1252200 AT2G27320.1 33.993 303 169 9 109 407 30 305 1.34e-38 142
Msa1252200 AT4G10220.1 27.791 421 255 15 41 442 8 398 1.40e-37 141
Msa1252200 AT4G15053.1 30.000 430 247 20 38 442 1 401 2.20e-37 141
Msa1252200 AT4G23373.1 35.200 250 130 9 47 295 7 225 2.98e-34 130
Msa1252200 AT5G46810.1 28.000 350 224 11 110 442 11 349 8.22e-33 127
Msa1252200 AT5G05030.1 29.167 360 224 12 94 444 22 359 9.51e-33 127
Msa1252200 AT5G46820.1 28.732 355 225 12 101 442 8 347 1.72e-32 126
Msa1252200 AT5G25410.1 28.056 360 224 13 94 442 28 363 5.37e-32 125
Msa1252200 AT5G60380.1 29.446 343 213 14 110 442 44 367 5.56e-30 120
Msa1252200 AT4G10210.1 27.119 413 247 16 38 427 5 386 1.59e-29 119
Msa1252200 AT5G46200.1 27.532 385 217 15 110 442 31 405 1.70e-29 119
Msa1252200 AT2G38255.1 34.762 210 103 6 110 312 14 196 3.91e-28 111
Msa1252200 AT4G23080.1 27.570 428 255 20 42 444 9 406 4.05e-27 112
Msa1252200 AT5G11660.1 30.717 293 181 11 160 444 26 304 3.15e-26 108
Msa1252200 AT4G17860.1 29.545 352 200 17 110 442 29 351 6.08e-26 108
Msa1252200 AT5G25415.1 32.377 244 144 8 212 444 99 332 4.81e-24 102
Msa1252200 AT5G36680.1 28.477 302 180 12 160 447 52 331 3.29e-23 100
Msa1252200 AT5G36770.1 28.477 302 180 12 160 447 52 331 3.29e-23 100
Msa1252200 AT2G03930.2 53.125 96 42 3 197 289 38 133 4.45e-23 95.9
Msa1252200 AT5G04937.1 29.442 197 112 8 253 442 22 198 6.79e-17 79.3
Msa1252200 AT5G37520.1 28.879 232 124 12 218 443 2 198 6.80e-15 73.6

Find 125 sgRNAs with CRISPR-Local

Find 301 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTATTCACACACTTAA+AGG 0.115660 8_2:-49022568 Msa1252200:CDS
AATATTCTTCTTTCATTTCT+TGG 0.122119 8_2:-49022203 Msa1252200:three_prime_UTR
TATGGATCTGGAAACTTAAT+TGG 0.136885 8_2:-49022603 Msa1252200:CDS
ATTTGAGTGTTCTTATATTT+TGG 0.152354 8_2:+49027158 None:intergenic
ACCCTAGATTCTTCACTTAT+TGG 0.171239 8_2:-49024637 Msa1252200:CDS
GTTTCTTCTCCTTGTTTCTT+TGG 0.178680 8_2:-49027039 Msa1252200:CDS
TCTCTCTCTGAACATATTTC+AGG 0.180748 8_2:+49027315 None:intergenic
TCTTGTATATTATAACAATT+TGG 0.235227 8_2:+49022348 None:intergenic
GGCTTCAAAGGCTTTGAAAA+TGG 0.257111 8_2:-49027274 Msa1252200:five_prime_UTR
TCTTCTTTCATTTCTTGGTT+GGG 0.261381 8_2:-49022198 Msa1252200:three_prime_UTR
ATCCTTTAAGTGTGTGAATA+AGG 0.263709 8_2:+49022566 None:intergenic
ATGAAGAGTTTCACAAGTTT+AGG 0.287405 8_2:-49026962 Msa1252200:CDS
TCTCCAACTTCTACATATAA+TGG 0.288121 8_2:-49024388 Msa1252200:CDS
AATTCAGCTTGTCTCAAATA+TGG 0.288451 8_2:-49025276 Msa1252200:CDS
CCCATTTGCTTATGCTCTCT+TGG 0.302334 8_2:+49026582 None:intergenic
TTGCAAAAGCTGCATACTTT+AGG 0.303212 8_2:-49022440 Msa1252200:CDS
GGGAATTGGTGGCTAGAATA+TGG 0.311539 8_2:-49022621 Msa1252200:CDS
CAAAATGTCCACTACCCATT+TGG 0.312408 8_2:+49022475 None:intergenic
ATACAAGAAGGAACTAATAA+TGG 0.313088 8_2:-49022333 Msa1252200:CDS
TTGGTAGAAAAGTTTGATTT+TGG 0.315468 8_2:+49026985 None:intergenic
TTCTTCTTTCATTTCTTGGT+TGG 0.328319 8_2:-49022199 Msa1252200:three_prime_UTR
AGTGCAACTATGGTTCAATT+TGG 0.335293 8_2:-49022543 Msa1252200:CDS
AGCCACCAATTCCCATGCTT+TGG 0.335334 8_2:+49022630 None:intergenic
TGGATCATCTCTGGTTCATT+TGG 0.338622 8_2:-49025256 Msa1252200:CDS
GCTTGCTCTATTAACACATT+TGG 0.344553 8_2:-49026459 Msa1252200:CDS
TTAGCTACAACATTGAGATT+TGG 0.370876 8_2:+49022375 None:intergenic
ATACATAATTATAATTATAT+AGG 0.371324 8_2:-49022244 Msa1252200:three_prime_UTR
CTTAAAGGATAGTGCAACTA+TGG 0.372236 8_2:-49022553 Msa1252200:CDS
TGCTACAATTTACTTTGCTC+TGG 0.378764 8_2:-49024454 Msa1252200:CDS
GTCCAATAAGTGAAGAATCT+AGG 0.380593 8_2:+49024635 None:intergenic
AAAATGTCCACTACCCATTT+GGG 0.385012 8_2:+49022476 None:intergenic
ACTAGCAGTAATATTGCAAT+TGG 0.395249 8_2:-49024421 Msa1252200:CDS
CTTCTTTCATTTCTTGGTTG+GGG 0.396638 8_2:-49022197 Msa1252200:three_prime_UTR
CTGGAAACTTAATTGGGTAT+TGG 0.396892 8_2:-49022596 Msa1252200:CDS
TTGATATTAGCTTAATGATC+TGG 0.397300 8_2:-49024357 Msa1252200:CDS
TTAGATTGTCTTACAAATAC+TGG 0.397633 8_2:-49027240 Msa1252200:five_prime_UTR
ACTGATGCATATCAAGGAAC+TGG 0.402596 8_2:-49024478 Msa1252200:CDS
AACTTGTGAAACTCTTCATT+TGG 0.403131 8_2:+49026966 None:intergenic
CAGGTCAGCCCAGAGCTCTA+TGG 0.408690 8_2:-49024668 Msa1252200:CDS
TCATTAAGCTAATATCAAAT+TGG 0.410645 8_2:+49024362 None:intergenic
TTTCAATTGTGGAGTTTCTC+AGG 0.412909 8_2:-49026543 Msa1252200:CDS
CTAATCGGAATCGTTCCTTC+TGG 0.418990 8_2:+49026510 None:intergenic
TGAGACAAGCTGAATTCATA+TGG 0.421304 8_2:+49025283 None:intergenic
ATAGTGAACACAATGTCAAC+AGG 0.427759 8_2:-49022513 Msa1252200:CDS
AAATATGTTCAGAGAGAGAA+AGG 0.428067 8_2:-49027311 Msa1252200:five_prime_UTR
ACCAAGAGAGCATAAGCAAA+TGG 0.428963 8_2:-49026583 Msa1252200:CDS
GGCTCACACATCAACCCAAA+TGG 0.429520 8_2:-49022490 Msa1252200:CDS
CATATGGACTTTCCACTTGT+GGG 0.431251 8_2:+49025299 None:intergenic
GCCTGCTGTTAAGACAATTC+AGG 0.432132 8_2:-49026904 Msa1252200:intron
CTCTCTCTGAACATATTTCA+GGG 0.432830 8_2:+49027316 None:intergenic
TACAAGAAGGAACTAATAAT+GGG 0.448624 8_2:-49022332 Msa1252200:CDS
TAGCTATCCCCATAGAGCTC+TGG 0.454983 8_2:+49024659 None:intergenic
AAAATTGAAACGTGTTAGAA+TGG 0.456346 8_2:-49026433 Msa1252200:CDS
TACCATTGAAGCTGGTTGGC+AGG 0.466050 8_2:-49025221 Msa1252200:intron
CCTCCATTATATGTAGAAGT+TGG 0.467832 8_2:+49024385 None:intergenic
AGCTATCCCCATAGAGCTCT+GGG 0.473291 8_2:+49024660 None:intergenic
AGGATCCAAAGCATGGGAAT+TGG 0.479599 8_2:-49022635 Msa1252200:CDS
GCAACTATGGTTCAATTTGG+TGG 0.479885 8_2:-49022540 Msa1252200:CDS
TGCCTGCCAACCAGCTTCAA+TGG 0.481927 8_2:+49025219 None:intergenic
AGGTCAGCCCAGAGCTCTAT+GGG 0.482474 8_2:-49024667 Msa1252200:CDS
ACCTGAATTGTCTTAACAGC+AGG 0.484466 8_2:+49026903 None:intergenic
GTGAGTGGGAATGAGTACTA+TGG 0.487457 8_2:-49025349 Msa1252200:CDS
AAAATTATAACTTTCAATTG+TGG 0.487536 8_2:-49026554 Msa1252200:CDS
TGAAGGAGGAGGGAGATGAC+TGG 0.493433 8_2:+49027062 None:intergenic
GGAGAAGAAACTGAAGGAGG+AGG 0.496862 8_2:+49027051 None:intergenic
TTTGATCATCCTATGTTGAA+AGG 0.498574 8_2:-49026737 Msa1252200:CDS
ATGGATCTGGAAACTTAATT+GGG 0.501045 8_2:-49022602 Msa1252200:CDS
TCTCAAATATGGATCATCTC+TGG 0.501761 8_2:-49025265 Msa1252200:CDS
CTTTAGGAATATGCAAGTTG+TGG 0.502120 8_2:-49022424 Msa1252200:CDS
TCCAATAAGTGAAGAATCTA+GGG 0.502742 8_2:+49024636 None:intergenic
TGGTGGCTAGAATATGGATC+TGG 0.504803 8_2:-49022615 Msa1252200:CDS
CCAAGAGAGCATAAGCAAAT+GGG 0.509356 8_2:-49026582 Msa1252200:CDS
AACATAGGATGATCAAAAGC+TGG 0.509432 8_2:+49026743 None:intergenic
TTATGCTCTCTTGGTCTCTC+CGG 0.511099 8_2:+49026591 None:intergenic
TCATATGGACTTTCCACTTG+TGG 0.512196 8_2:+49025298 None:intergenic
TGGGGTTAGATTTGTTCCAA+TGG 0.524090 8_2:-49027187 Msa1252200:CDS
ATCCAAAGCATGGGAATTGG+TGG 0.528426 8_2:-49022632 Msa1252200:CDS
TTGCAGACTGATGCATATCA+AGG 0.537288 8_2:-49024484 Msa1252200:intron
TTTATGTGTATAGGATCCTC+CGG 0.539657 8_2:-49026610 Msa1252200:intron
GCTCACACATCAACCCAAAT+GGG 0.541941 8_2:-49022489 Msa1252200:CDS
CAATCTATGATATCACCATC+AGG 0.544949 8_2:+49026785 None:intergenic
AAGAGTTTCACAAGTTTAGG+AGG 0.546074 8_2:-49026959 Msa1252200:CDS
GAAGGAACTAATAATGGGTG+GGG 0.546366 8_2:-49022327 Msa1252200:CDS
TTGTACCTCATGTCCATCGC+CGG 0.547979 8_2:+49026404 None:intergenic
GATCTCAATACCATTGAAGC+TGG 0.548557 8_2:-49025229 Msa1252200:CDS
TCAATACCATTGAAGCTGGT+TGG 0.549014 8_2:-49025225 Msa1252200:CDS
AGAAGGAACTAATAATGGGT+GGG 0.554565 8_2:-49022328 Msa1252200:CDS
TACAACATTGAGATTTGGCA+AGG 0.560466 8_2:+49022380 None:intergenic
AAGTGTGTGAATAAGGAAGA+TGG 0.561354 8_2:+49022573 None:intergenic
TGCGTGTAGGATCCAAAGCA+TGG 0.565093 8_2:-49022642 Msa1252200:intron
AAATGTGTGGGTCCCACAAG+TGG 0.566747 8_2:-49025311 Msa1252200:CDS
GTATATTATAACAATTTGGA+CGG 0.567975 8_2:+49022352 None:intergenic
TAGATTCTTCACTTATTGGA+CGG 0.568545 8_2:-49024633 Msa1252200:intron
TTTACTATGGTGGTCCTGGA+AGG 0.570062 8_2:-49022296 Msa1252200:CDS
AATGATGCACAGCATGCAGT+TGG 0.572287 8_2:-49025376 Msa1252200:intron
GAAGGAGGAGGGAGATGACT+GGG 0.580270 8_2:+49027063 None:intergenic
ACATCAACCCAAATGGGTAG+TGG 0.582932 8_2:-49022483 Msa1252200:CDS
TGCTCTCTTGGTCTCTCCGG+AGG 0.583271 8_2:+49026594 None:intergenic
CAAAAGCTAGCATAAATGTG+TGG 0.585357 8_2:-49025324 Msa1252200:CDS
AGAATGGACACCACCGGCGA+TGG 0.585596 8_2:-49026417 Msa1252200:CDS
AATTGTTATAATATACAAGA+AGG 0.590507 8_2:-49022345 Msa1252200:CDS
GTTTATTCTTCAGAAACAAG+TGG 0.596638 8_2:-49027008 Msa1252200:CDS
GAGAAGAAACTGAAGGAGGA+GGG 0.598952 8_2:+49027052 None:intergenic
CACCACCGGCGATGGACATG+AGG 0.599402 8_2:-49026409 Msa1252200:intron
GCAAGAAAACAACACAAATG+TGG 0.599836 8_2:-49027122 Msa1252200:CDS
AAACAAGGAGAAGAAACTGA+AGG 0.600954 8_2:+49027045 None:intergenic
CTGATGCATATCAAGGAACT+GGG 0.602205 8_2:-49024477 Msa1252200:CDS
AAGAAGGAACTAATAATGGG+TGG 0.603081 8_2:-49022329 Msa1252200:CDS
AATATGTTCAGAGAGAGAAA+GGG 0.604579 8_2:-49027310 Msa1252200:five_prime_UTR
TACCTCATGTCCATCGCCGG+TGG 0.612456 8_2:+49026407 None:intergenic
AAAAGCTAGCATAAATGTGT+GGG 0.619969 8_2:-49025323 Msa1252200:CDS
TATTAGCTTAATGATCTGGA+AGG 0.620476 8_2:-49024353 Msa1252200:intron
CATGACAAACCAAAGAAACA+AGG 0.620517 8_2:+49027030 None:intergenic
TCAGGTGAATCATGTCCAGA+AGG 0.626410 8_2:-49026525 Msa1252200:CDS
AATACACACAAACACAAACA+TGG 0.626698 8_2:-49027096 Msa1252200:CDS
GCGTGTAGGATCCAAAGCAT+GGG 0.644257 8_2:-49022641 Msa1252200:intron
ACAACATTGAGATTTGGCAA+GGG 0.654257 8_2:+49022381 None:intergenic
CGTGTTAGAATGGACACCAC+CGG 0.654611 8_2:-49026423 Msa1252200:CDS
CAAGGAGAAGAAACTGAAGG+AGG 0.658718 8_2:+49027048 None:intergenic
CCAACTTCTACATATAATGG+AGG 0.662547 8_2:-49024385 Msa1252200:CDS
ATGCAGTTGGATATGTGAGT+GGG 0.674700 8_2:-49025363 Msa1252200:CDS
CATGCAGTTGGATATGTGAG+TGG 0.677188 8_2:-49025364 Msa1252200:CDS
TAGTGAACACAATGTCAACA+GGG 0.694440 8_2:-49022512 Msa1252200:CDS
GGTCAGCCCAGAGCTCTATG+GGG 0.745363 8_2:-49024666 Msa1252200:CDS
AGTGAACACAATGTCAACAG+GGG 0.745728 8_2:-49022511 Msa1252200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTGAAATAATTAATAT+CGG - chr8_2:49023675-49023694 Msa1252200:intron 10.0%
!!! TTTTATCAGAATATTATTTT+TGG + chr8_2:49023884-49023903 None:intergenic 10.0%
!! TGTATTTAAAAAAATGATAA+TGG - chr8_2:49025013-49025032 Msa1252200:intron 10.0%
!! AAAAAATATGATAAATTATG+TGG + chr8_2:49026118-49026137 None:intergenic 10.0%
!! AAAAATAAAGGAATAAAAAA+AGG + chr8_2:49026230-49026249 None:intergenic 10.0%
!!! GTCATATATTATATTTTTGT+TGG - chr8_2:49022312-49022331 Msa1252200:CDS 15.0%
!!! ATATTATATTTTTGTTGGAT+TGG - chr8_2:49022317-49022336 Msa1252200:CDS 15.0%
!!! CATCTATTTTTTCTTAAAAA+AGG + chr8_2:49022684-49022703 None:intergenic 15.0%
!!! CTTTTTTAAGAAAAAATAGA+TGG - chr8_2:49022682-49022701 Msa1252200:intron 15.0%
!!! AGTTTTTTTTATAGATCAAA+AGG - chr8_2:49022840-49022859 Msa1252200:intron 15.0%
!! TATTTCAACTTATAATTGTT+CGG - chr8_2:49022896-49022915 Msa1252200:intron 15.0%
!! AAAATTATAACTTTCAATTG+TGG - chr8_2:49022976-49022995 Msa1252200:intron 15.0%
!!! TTTTATGTTTCTTTTCATTA+TGG - chr8_2:49023214-49023233 Msa1252200:intron 15.0%
!!! TTTTGGCTTATATAAAATTA+CGG - chr8_2:49023383-49023402 Msa1252200:intron 15.0%
!! AAAGAAAATATAGACAAAAA+TGG + chr8_2:49023470-49023489 None:intergenic 15.0%
!! ATTATTTCAAAAGAAATGAA+TGG + chr8_2:49023669-49023688 None:intergenic 15.0%
!! TAAAAAGAAAAGAAAACATT+TGG + chr8_2:49023717-49023736 None:intergenic 15.0%
!! CTATTGAAGATAAAAATAAT+TGG + chr8_2:49024038-49024057 None:intergenic 15.0%
!! ATTTATATTAGATTCAATGT+TGG + chr8_2:49024085-49024104 None:intergenic 15.0%
!!! ATTTTTTACTTGTCATTTTA+AGG - chr8_2:49024426-49024445 Msa1252200:CDS 15.0%
!!! AAAGTATCATTATTAAGTAT+TGG + chr8_2:49024570-49024589 None:intergenic 15.0%
!! TTTATACATATCAAGAAAAA+TGG + chr8_2:49024625-49024644 None:intergenic 15.0%
!! AACAAATAGAACAATTAATT+TGG - chr8_2:49024648-49024667 Msa1252200:CDS 15.0%
!!! CAATGTTTTTAATTTTGTAT+TGG - chr8_2:49024808-49024827 Msa1252200:intron 15.0%
!! TTAAAAATTGTTTATAAACG+TGG - chr8_2:49025396-49025415 Msa1252200:intron 15.0%
!!! AATATTTTGTAACAAATCAA+TGG + chr8_2:49025673-49025692 None:intergenic 15.0%
!! TGAAAAAAAGAAACAAAAAA+GGG + chr8_2:49026095-49026114 None:intergenic 15.0%
!! GTTAAATAAGAGAAAAATAA+AGG + chr8_2:49026242-49026261 None:intergenic 15.0%
!! TTAACATATTAACATGATAA+AGG - chr8_2:49026655-49026674 Msa1252200:intron 15.0%
!!! TACTTTTCGTTTTTAAATTA+CGG + chr8_2:49026736-49026755 None:intergenic 15.0%
!! TCTTGTATATTATAACAATT+TGG + chr8_2:49027185-49027204 None:intergenic 15.0%
!! AATTGTTATAATATACAAGA+AGG - chr8_2:49027185-49027204 Msa1252200:CDS 15.0%
!!! ATGTATTATTCATTTTCTTA+GGG + chr8_2:49027272-49027291 None:intergenic 15.0%
!!! TATGTATTATTCATTTTCTT+AGG + chr8_2:49027273-49027292 None:intergenic 15.0%
!!! ATTTGAGTGTTCTTATATTT+TGG + chr8_2:49022375-49022394 None:intergenic 20.0%
!! AAAACTAAGAAAAATACCAA+TGG + chr8_2:49022826-49022845 None:intergenic 20.0%
!!! TCTTCTTTTATTCTTCTAAT+CGG + chr8_2:49023038-49023057 None:intergenic 20.0%
!!! TCTATGTTAAGTTGTTTTTT+GGG + chr8_2:49023275-49023294 None:intergenic 20.0%
!!! ATCTATGTTAAGTTGTTTTT+TGG + chr8_2:49023276-49023295 None:intergenic 20.0%
!! AACTTAACATAGATAGATAA+TGG - chr8_2:49023282-49023301 Msa1252200:intron 20.0%
!!! CATATGGTAGTATTTTTTTA+GGG - chr8_2:49023317-49023336 Msa1252200:intron 20.0%
!!! ATTTTACGTTAAATGTGTTT+TGG - chr8_2:49023366-49023385 Msa1252200:intron 20.0%
!!! AAATGGTCAAATTTCTTTTA+CGG + chr8_2:49023453-49023472 None:intergenic 20.0%
!!! ATAAAGTACTAACAACAAAT+TGG - chr8_2:49024123-49024142 Msa1252200:intron 20.0%
!!! TCATTATTAAGTATTGGATT+TGG + chr8_2:49024564-49024583 None:intergenic 20.0%
!! TATCAAGAAAAATGGATAAA+TGG + chr8_2:49024617-49024636 None:intergenic 20.0%
!!! AACAATTAATTTGGAAAGAA+GGG - chr8_2:49024657-49024676 Msa1252200:CDS 20.0%
!! ACGAAAATTCTTAAATTGAT+TGG - chr8_2:49024727-49024746 Msa1252200:intron 20.0%
!! ATTCTTAAATTGATTGGATT+TGG - chr8_2:49024733-49024752 Msa1252200:intron 20.0%
!! AAAACATTGATGAAAATGAA+AGG + chr8_2:49024797-49024816 None:intergenic 20.0%
!!! AGTTTAAGTAATTTTTATGC+AGG - chr8_2:49024843-49024862 Msa1252200:intron 20.0%
!! TCATTAAGCTAATATCAAAT+TGG + chr8_2:49025171-49025190 None:intergenic 20.0%
!! AAATTGCTTATAAATGTGAT+CGG + chr8_2:49025295-49025314 None:intergenic 20.0%
!!! AATAATCTCATTGACTAAAT+TGG + chr8_2:49025567-49025586 None:intergenic 20.0%
!!! AAATCAATGGTTAGTTAATT+TGG + chr8_2:49025660-49025679 None:intergenic 20.0%
!! GTGAAAAAAAGAAACAAAAA+AGG + chr8_2:49026096-49026115 None:intergenic 20.0%
!!! GTAAAATACGCAAAATTTTA+GGG + chr8_2:49026269-49026288 None:intergenic 20.0%
!!! AGTAAAATACGCAAAATTTT+AGG + chr8_2:49026270-49026289 None:intergenic 20.0%
!! AAGGTTTATATTTCAATGTT+AGG - chr8_2:49026540-49026559 Msa1252200:CDS 20.0%
!! AGGTTTATATTTCAATGTTA+GGG - chr8_2:49026541-49026560 Msa1252200:CDS 20.0%
!! GTATATTATAACAATTTGGA+CGG + chr8_2:49027181-49027200 None:intergenic 20.0%
!! AATATTCTTCTTTCATTTCT+TGG - chr8_2:49027327-49027346 Msa1252200:five_prime_UTR 20.0%
! TTAGATTGTCTTACAAATAC+TGG - chr8_2:49022290-49022309 Msa1252200:CDS 25.0%
!!! TATTTTTGTTGGATTGGATA+TGG - chr8_2:49022323-49022342 Msa1252200:CDS 25.0%
!!! ATTTTTGTTGGATTGGATAT+GGG - chr8_2:49022324-49022343 Msa1252200:CDS 25.0%
!!! TATATTTTGGTTTTGTCCAT+TGG + chr8_2:49022362-49022381 None:intergenic 25.0%
!!! TTGGTAGAAAAGTTTGATTT+TGG + chr8_2:49022548-49022567 None:intergenic 25.0%
! AAAATTGAAACGTGTTAGAA+TGG - chr8_2:49023097-49023116 Msa1252200:intron 25.0%
!! CTTTTCATTATGGTAAAGTA+TGG - chr8_2:49023224-49023243 Msa1252200:intron 25.0%
!!! CATAGATAGATAATGGAAAT+TGG - chr8_2:49023289-49023308 Msa1252200:intron 25.0%
!!! GCATATGGTAGTATTTTTTT+AGG - chr8_2:49023316-49023335 Msa1252200:intron 25.0%
!!! TTTTTTTAGGGTAAAGCATA+TGG - chr8_2:49023329-49023348 Msa1252200:intron 25.0%
!!! AGTCCTTTTTCAATTTAAAG+TGG - chr8_2:49023965-49023984 Msa1252200:intron 25.0%
! TGACCACTTTAAATTGAAAA+AGG + chr8_2:49023971-49023990 None:intergenic 25.0%
! CTTCTCAGTTTATTAATATG+TGG - chr8_2:49024011-49024030 Msa1252200:intron 25.0%
! ATGACAAGTAAAAAATGAGT+GGG + chr8_2:49024422-49024441 None:intergenic 25.0%
! AATGACAAGTAAAAAATGAG+TGG + chr8_2:49024423-49024442 None:intergenic 25.0%
!! GAACAATTAATTTGGAAAGA+AGG - chr8_2:49024656-49024675 Msa1252200:CDS 25.0%
!!! GATTTGGTTTTGCATTAAAT+AGG - chr8_2:49024749-49024768 Msa1252200:intron 25.0%
! TAAGTAAATTGATTGAAGCA+AGG - chr8_2:49024920-49024939 Msa1252200:intron 25.0%
! AAGTAAATTGATTGAAGCAA+GGG - chr8_2:49024921-49024940 Msa1252200:intron 25.0%
!! TTGATATTAGCTTAATGATC+TGG - chr8_2:49025173-49025192 Msa1252200:intron 25.0%
!!! ATAATCTTCTAAGGTCATTT+TGG + chr8_2:49025222-49025241 None:intergenic 25.0%
! AAAATGACCTTAGAAGATTA+TGG - chr8_2:49025221-49025240 Msa1252200:intron 25.0%
! AAATGACCTTAGAAGATTAT+GGG - chr8_2:49025222-49025241 Msa1252200:CDS 25.0%
! AAATTACCCATAATCTTCTA+AGG + chr8_2:49025231-49025250 None:intergenic 25.0%
! TTAGAAGATTATGGGTAATT+TGG - chr8_2:49025230-49025249 Msa1252200:CDS 25.0%
! GAAAATTTAAGCTGAAAATG+TGG - chr8_2:49025719-49025738 Msa1252200:intron 25.0%
! GTTCAAATGTTAGTGAATTT+AGG - chr8_2:49025950-49025969 Msa1252200:intron 25.0%
! AGTAGTAAAGTGAGTAAATA+TGG + chr8_2:49025978-49025997 None:intergenic 25.0%
! ATTTACTCACTTTACTACTT+TGG - chr8_2:49025979-49025998 Msa1252200:intron 25.0%
!! GAAAGAGATTAAAAGCATAA+GGG + chr8_2:49026042-49026061 None:intergenic 25.0%
!! TGAAAGAGATTAAAAGCATA+AGG + chr8_2:49026043-49026062 None:intergenic 25.0%
!!! CTTTAAGTTGTAAAAAGCAA+AGG + chr8_2:49026183-49026202 None:intergenic 25.0%
! TAAAGGAATAAAAAAAGGTG+TGG + chr8_2:49026225-49026244 None:intergenic 25.0%
! AGTCAAAAAGAAGAAAACAA+AGG + chr8_2:49026306-49026325 None:intergenic 25.0%
! AACACCATACATAACATTAA+AGG + chr8_2:49026803-49026822 None:intergenic 25.0%
! ATACAAGAAGGAACTAATAA+TGG - chr8_2:49027197-49027216 Msa1252200:exon 25.0%
! TACAAGAAGGAACTAATAAT+GGG - chr8_2:49027198-49027217 Msa1252200:exon 25.0%
AAATATGTTCAGAGAGAGAA+AGG - chr8_2:49022219-49022238 Msa1252200:three_prime_UTR 30.0%
AATATGTTCAGAGAGAGAAA+GGG - chr8_2:49022220-49022239 Msa1252200:three_prime_UTR 30.0%
!! TTTTTGTTGGATTGGATATG+GGG - chr8_2:49022325-49022344 Msa1252200:CDS 30.0%
AATACACACAAACACAAACA+TGG - chr8_2:49022434-49022453 Msa1252200:CDS 30.0%
GTTTATTCTTCAGAAACAAG+TGG - chr8_2:49022522-49022541 Msa1252200:CDS 30.0%
AACTTGTGAAACTCTTCATT+TGG + chr8_2:49022567-49022586 None:intergenic 30.0%
ATGAAGAGTTTCACAAGTTT+AGG - chr8_2:49022568-49022587 Msa1252200:CDS 30.0%
TTTGATCATCCTATGTTGAA+AGG - chr8_2:49022793-49022812 Msa1252200:intron 30.0%
!! TTGTTCGGTTTTATGTGTAT+AGG - chr8_2:49022911-49022930 Msa1252200:intron 30.0%
ATGGAAATTGGTAAAGCATA+TGG - chr8_2:49023301-49023320 Msa1252200:intron 30.0%
TGGCTTATATAAAATTACGG+TGG - chr8_2:49023386-49023405 Msa1252200:intron 30.0%
! AGACTAACTTTTCTTTTGGA+AGG + chr8_2:49023430-49023449 None:intergenic 30.0%
!!! GTGATTTTAGCTTCTACATA+AGG - chr8_2:49023500-49023519 Msa1252200:intron 30.0%
TGTATTAATGTGAGACTACA+AGG + chr8_2:49023834-49023853 None:intergenic 30.0%
AAAAGCTAGCATAAATGTGT+GGG - chr8_2:49024207-49024226 Msa1252200:intron 30.0%
AATTCAGCTTGTCTCAAATA+TGG - chr8_2:49024254-49024273 Msa1252200:intron 30.0%
!!! TTTGCACTGCTTTTTTTAGT+AGG - chr8_2:49024392-49024411 Msa1252200:CDS 30.0%
TGATGAAAATGAAAGGTAGT+TGG + chr8_2:49024790-49024809 None:intergenic 30.0%
TCCAATAAGTGAAGAATCTA+GGG + chr8_2:49024897-49024916 None:intergenic 30.0%
TAGATTCTTCACTTATTGGA+CGG - chr8_2:49024897-49024916 Msa1252200:intron 30.0%
ACTAGCAGTAATATTGCAAT+TGG - chr8_2:49025109-49025128 Msa1252200:intron 30.0%
TCTCCAACTTCTACATATAA+TGG - chr8_2:49025142-49025161 Msa1252200:intron 30.0%
! TATTAGCTTAATGATCTGGA+AGG - chr8_2:49025177-49025196 Msa1252200:intron 30.0%
TAGTAAATACCAGAGAAACT+TGG + chr8_2:49025267-49025286 None:intergenic 30.0%
TAATGAGACTTCCAAACTAA+TGG + chr8_2:49025773-49025792 None:intergenic 30.0%
TAGAATTGTAGAAGAAGCAA+GGG - chr8_2:49025822-49025841 Msa1252200:intron 30.0%
TGTATCGTTGAAGATTTACT+AGG - chr8_2:49026412-49026431 Msa1252200:CDS 30.0%
GTATCGTTGAAGATTTACTA+GGG - chr8_2:49026413-49026432 Msa1252200:CDS 30.0%
TAATATAACCACATTGAACC+CGG + chr8_2:49026487-49026506 None:intergenic 30.0%
! CATAACATTAAAGGTGTGTT+TGG + chr8_2:49026794-49026813 None:intergenic 30.0%
CACACCTTTAATGTTATGTA+TGG - chr8_2:49026796-49026815 Msa1252200:intron 30.0%
TATGGATCTGGAAACTTAAT+TGG - chr8_2:49026927-49026946 Msa1252200:CDS 30.0%
ATGGATCTGGAAACTTAATT+GGG - chr8_2:49026928-49026947 Msa1252200:CDS 30.0%
TTCCTTATTCACACACTTAA+AGG - chr8_2:49026962-49026981 Msa1252200:CDS 30.0%
ATCCTTTAAGTGTGTGAATA+AGG + chr8_2:49026967-49026986 None:intergenic 30.0%
TTAGCTACAACATTGAGATT+TGG + chr8_2:49027158-49027177 None:intergenic 30.0%
!! TGGGGAAATTACTTTTACTA+TGG - chr8_2:49027221-49027240 Msa1252200:five_prime_UTR 30.0%
!! GGAAATTACTTTTACTATGG+TGG - chr8_2:49027224-49027243 Msa1252200:five_prime_UTR 30.0%
TTCTTCTTTCATTTCTTGGT+TGG - chr8_2:49027331-49027350 Msa1252200:five_prime_UTR 30.0%
TCTTCTTTCATTTCTTGGTT+GGG - chr8_2:49027332-49027351 Msa1252200:five_prime_UTR 30.0%
CTCTCTCTGAACATATTTCA+GGG + chr8_2:49022217-49022236 None:intergenic 35.0%
TCTCTCTCTGAACATATTTC+AGG + chr8_2:49022218-49022237 None:intergenic 35.0%
GCAAGAAAACAACACAAATG+TGG - chr8_2:49022408-49022427 Msa1252200:CDS 35.0%
AAACAAGGAGAAGAAACTGA+AGG + chr8_2:49022488-49022507 None:intergenic 35.0%
! GTTTCTTCTCCTTGTTTCTT+TGG - chr8_2:49022491-49022510 Msa1252200:CDS 35.0%
CATGACAAACCAAAGAAACA+AGG + chr8_2:49022503-49022522 None:intergenic 35.0%
AAGAGTTTCACAAGTTTAGG+AGG - chr8_2:49022571-49022590 Msa1252200:CDS 35.0%
CCATAAAAATGCATCTTCGA+CGG - chr8_2:49022595-49022614 Msa1252200:CDS 35.0%
CAATCTATGATATCACCATC+AGG + chr8_2:49022748-49022767 None:intergenic 35.0%
AACATAGGATGATCAAAAGC+TGG + chr8_2:49022790-49022809 None:intergenic 35.0%
! GGTTTTTGTCCTTTCAACAT+AGG + chr8_2:49022805-49022824 None:intergenic 35.0%
GTTGAAAGGACAAAAACCAT+TGG - chr8_2:49022807-49022826 Msa1252200:intron 35.0%
TTTATGTGTATAGGATCCTC+CGG - chr8_2:49022920-49022939 Msa1252200:intron 35.0%
TTTCAATTGTGGAGTTTCTC+AGG - chr8_2:49022987-49023006 Msa1252200:intron 35.0%
GCTTGCTCTATTAACACATT+TGG - chr8_2:49023071-49023090 Msa1252200:intron 35.0%
!!! GTTTCTGTTGTTTTCTGTGA+TGG + chr8_2:49023154-49023173 None:intergenic 35.0%
! ACGGAGACTAACTTTTCTTT+TGG + chr8_2:49023434-49023453 None:intergenic 35.0%
ATTAATGTGAGACTACAAGG+AGG + chr8_2:49023831-49023850 None:intergenic 35.0%
AAAGTGGTCACTGAATTTCA+TGG - chr8_2:49023981-49024000 Msa1252200:intron 35.0%
CAAAAGCTAGCATAAATGTG+TGG - chr8_2:49024206-49024225 Msa1252200:intron 35.0%
TGAGACAAGCTGAATTCATA+TGG + chr8_2:49024250-49024269 None:intergenic 35.0%
TCTCAAATATGGATCATCTC+TGG - chr8_2:49024265-49024284 Msa1252200:intron 35.0%
ACCCTAGATTCTTCACTTAT+TGG - chr8_2:49024893-49024912 Msa1252200:intron 35.0%
GTCCAATAAGTGAAGAATCT+AGG + chr8_2:49024898-49024917 None:intergenic 35.0%
TAAATTGATTGAAGCAAGGG+AGG - chr8_2:49024924-49024943 Msa1252200:intron 35.0%
TGCTACAATTTACTTTGCTC+TGG - chr8_2:49025076-49025095 Msa1252200:intron 35.0%
CCTCCATTATATGTAGAAGT+TGG + chr8_2:49025148-49025167 None:intergenic 35.0%
CCAACTTCTACATATAATGG+AGG - chr8_2:49025145-49025164 Msa1252200:intron 35.0%
AAATACCAGAGAAACTTGGA+GGG + chr8_2:49025263-49025282 None:intergenic 35.0%
TAAATACCAGAGAAACTTGG+AGG + chr8_2:49025264-49025283 None:intergenic 35.0%
TTGCTTATAAATGTGATCGG+AGG + chr8_2:49025292-49025311 None:intergenic 35.0%
TGTTTATAAACGTGGCAAGA+TGG - chr8_2:49025404-49025423 Msa1252200:intron 35.0%
TCTCATAGTCAAAGTCTCAA+AGG - chr8_2:49025697-49025716 Msa1252200:intron 35.0%
GTAGAATTGTAGAAGAAGCA+AGG - chr8_2:49025821-49025840 Msa1252200:intron 35.0%
! AAAGTGCTCAGAAAGAATGA+GGG + chr8_2:49026146-49026165 None:intergenic 35.0%
! AAAAAAAGGTGTGGCGTTTA+TGG + chr8_2:49026216-49026235 None:intergenic 35.0%
!! AGAAGAAAACAAAGGTACCA+AGG + chr8_2:49026298-49026317 None:intergenic 35.0%
TCAGATGATTAACAAGTGTC+CGG - chr8_2:49026465-49026484 Msa1252200:CDS 35.0%
TTTCAATGTTAGGGTGTGTT+TGG - chr8_2:49026550-49026569 Msa1252200:CDS 35.0%
! TAAAGGTGTGTTTGGATTGA+GGG + chr8_2:49026786-49026805 None:intergenic 35.0%
! TTAAAGGTGTGTTTGGATTG+AGG + chr8_2:49026787-49026806 None:intergenic 35.0%
CTGGAAACTTAATTGGGTAT+TGG - chr8_2:49026934-49026953 Msa1252200:CDS 35.0%
AAGTGTGTGAATAAGGAAGA+TGG + chr8_2:49026960-49026979 None:intergenic 35.0%
CTTAAAGGATAGTGCAACTA+TGG - chr8_2:49026977-49026996 Msa1252200:CDS 35.0%
AGTGCAACTATGGTTCAATT+TGG - chr8_2:49026987-49027006 Msa1252200:CDS 35.0%
ATAGTGAACACAATGTCAAC+AGG - chr8_2:49027017-49027036 Msa1252200:CDS 35.0%
TAGTGAACACAATGTCAACA+GGG - chr8_2:49027018-49027037 Msa1252200:CDS 35.0%
AAAATGTCCACTACCCATTT+GGG + chr8_2:49027057-49027076 None:intergenic 35.0%
TTGCAAAAGCTGCATACTTT+AGG - chr8_2:49027090-49027109 Msa1252200:CDS 35.0%
! CTTTAGGAATATGCAAGTTG+TGG - chr8_2:49027106-49027125 Msa1252200:CDS 35.0%
ACAACATTGAGATTTGGCAA+GGG + chr8_2:49027152-49027171 None:intergenic 35.0%
TACAACATTGAGATTTGGCA+AGG + chr8_2:49027153-49027172 None:intergenic 35.0%
! AAGAAGGAACTAATAATGGG+TGG - chr8_2:49027201-49027220 Msa1252200:exon 35.0%
!! AGAAGGAACTAATAATGGGT+GGG - chr8_2:49027202-49027221 Msa1252200:exon 35.0%
!!! AGAAAGGGAGAGCATTTTCT+TGG - chr8_2:49022235-49022254 Msa1252200:three_prime_UTR 40.0%
! GAGCATTTTCTTGGCTTCAA+AGG - chr8_2:49022244-49022263 Msa1252200:three_prime_UTR 40.0%
!! GGCTTCAAAGGCTTTGAAAA+TGG - chr8_2:49022256-49022275 Msa1252200:three_prime_UTR 40.0%
! TGGGGTTAGATTTGTTCCAA+TGG - chr8_2:49022343-49022362 Msa1252200:CDS 40.0%
! CCGTCGAAGATGCATTTTTA+TGG + chr8_2:49022598-49022617 None:intergenic 40.0%
ACCTGAATTGTCTTAACAGC+AGG + chr8_2:49022630-49022649 None:intergenic 40.0%
! ACCAAGAGAGCATAAGCAAA+TGG - chr8_2:49022947-49022966 Msa1252200:intron 40.0%
! CCAAGAGAGCATAAGCAAAT+GGG - chr8_2:49022948-49022967 Msa1252200:intron 40.0%
ATGCAGTTGGATATGTGAGT+GGG - chr8_2:49024167-49024186 Msa1252200:intron 40.0%
CATATGGACTTTCCACTTGT+GGG + chr8_2:49024234-49024253 None:intergenic 40.0%
TCATATGGACTTTCCACTTG+TGG + chr8_2:49024235-49024254 None:intergenic 40.0%
! TGGATCATCTCTGGTTCATT+TGG - chr8_2:49024274-49024293 Msa1252200:intron 40.0%
GATCTCAATACCATTGAAGC+TGG - chr8_2:49024301-49024320 Msa1252200:intron 40.0%
TCAATACCATTGAAGCTGGT+TGG - chr8_2:49024305-49024324 Msa1252200:intron 40.0%
TTGCAGACTGATGCATATCA+AGG - chr8_2:49025046-49025065 Msa1252200:intron 40.0%
ACTGATGCATATCAAGGAAC+TGG - chr8_2:49025052-49025071 Msa1252200:intron 40.0%
CTGATGCATATCAAGGAACT+GGG - chr8_2:49025053-49025072 Msa1252200:intron 40.0%
TATATCCCTCCAAGTTTCTC+TGG - chr8_2:49025255-49025274 Msa1252200:CDS 40.0%
! CTCATTGACTAAATTGGCAC+TGG + chr8_2:49025561-49025580 None:intergenic 40.0%
CTGAAAATGTGGTCAATGCA+TGG - chr8_2:49025730-49025749 Msa1252200:intron 40.0%
AAAATGTGGTCAATGCATGG+TGG - chr8_2:49025733-49025752 Msa1252200:intron 40.0%
AAATGTGGTCAATGCATGGT+GGG - chr8_2:49025734-49025753 Msa1252200:intron 40.0%
! CAAAGTGCTCAGAAAGAATG+AGG + chr8_2:49026147-49026166 None:intergenic 40.0%
! GCGTATTTTACTTACCACCT+TGG - chr8_2:49026278-49026297 Msa1252200:intron 40.0%
!! AGAAAACAAAGGTACCAAGG+TGG + chr8_2:49026295-49026314 None:intergenic 40.0%
TCTGACACACATTCAATCAG+AGG + chr8_2:49026450-49026469 None:intergenic 40.0%
CAGATGATTAACAAGTGTCC+GGG - chr8_2:49026466-49026485 Msa1252200:CDS 40.0%
AATGTTAGGGTGTGTTTGGA+TGG - chr8_2:49026554-49026573 Msa1252200:CDS 40.0%
TTGGATGGAGGATTTAGAAG+AGG - chr8_2:49026569-49026588 Msa1252200:CDS 40.0%
ATGGAGGATTTAGAAGAGGA+AGG - chr8_2:49026573-49026592 Msa1252200:CDS 40.0%
TGGAGGATTTAGAAGAGGAA+GGG - chr8_2:49026574-49026593 Msa1252200:CDS 40.0%
TGTGTTTGGATTGAGGGTTT+AGG + chr8_2:49026780-49026799 None:intergenic 40.0%
TTCCTTCTATTTGTGCGTGT+AGG - chr8_2:49026875-49026894 Msa1252200:intron 40.0%
ATCCTACACGCACAAATAGA+AGG + chr8_2:49026880-49026899 None:intergenic 40.0%
GCAACTATGGTTCAATTTGG+TGG - chr8_2:49026990-49027009 Msa1252200:CDS 40.0%
AGTGAACACAATGTCAACAG+GGG - chr8_2:49027019-49027038 Msa1252200:CDS 40.0%
CAAAATGTCCACTACCCATT+TGG + chr8_2:49027058-49027077 None:intergenic 40.0%
!! TAGTGGACATTTTGCTGAAG+AGG - chr8_2:49027064-49027083 Msa1252200:CDS 40.0%
! AGTGGACATTTTGCTGAAGA+GGG - chr8_2:49027065-49027084 Msa1252200:CDS 40.0%
!! GAAGGAACTAATAATGGGTG+GGG - chr8_2:49027203-49027222 Msa1252200:exon 40.0%
! TACTTTTACTATGGTGGTCC+TGG - chr8_2:49027230-49027249 Msa1252200:five_prime_UTR 40.0%
! GGACATTTTACATTCCTTCC+AGG + chr8_2:49027251-49027270 None:intergenic 40.0%
GAGAAGAAACTGAAGGAGGA+GGG + chr8_2:49022481-49022500 None:intergenic 45.0%
CAAGGAGAAGAAACTGAAGG+AGG + chr8_2:49022485-49022504 None:intergenic 45.0%
GCCTGCTGTTAAGACAATTC+AGG - chr8_2:49022626-49022645 Msa1252200:CDS 45.0%
ATATCACCATCAGGACTCTG+CGG + chr8_2:49022739-49022758 None:intergenic 45.0%
TTATGCTCTCTTGGTCTCTC+CGG + chr8_2:49022942-49022961 None:intergenic 45.0%
CCCATTTGCTTATGCTCTCT+TGG + chr8_2:49022951-49022970 None:intergenic 45.0%
TCAGGTGAATCATGTCCAGA+AGG - chr8_2:49023005-49023024 Msa1252200:intron 45.0%
CTAATCGGAATCGTTCCTTC+TGG + chr8_2:49023023-49023042 None:intergenic 45.0%
! AGACTACAAGGAGGATTGGT+TGG + chr8_2:49023822-49023841 None:intergenic 45.0%
TGTGAGACTACAAGGAGGAT+TGG + chr8_2:49023826-49023845 None:intergenic 45.0%
AATGATGCACAGCATGCAGT+TGG - chr8_2:49024154-49024173 Msa1252200:intron 45.0%
CATGCAGTTGGATATGTGAG+TGG - chr8_2:49024166-49024185 Msa1252200:intron 45.0%
GTGAGTGGGAATGAGTACTA+TGG - chr8_2:49024181-49024200 Msa1252200:intron 45.0%
ATTGATTGAAGCAAGGGAGG+TGG - chr8_2:49024927-49024946 Msa1252200:intron 45.0%
AATGTGGTCAATGCATGGTG+GGG - chr8_2:49025735-49025754 Msa1252200:intron 45.0%
AAGGTGTGGCGTTTATGGAA+TGG + chr8_2:49026211-49026230 None:intergenic 45.0%
AGGATTTAGAAGAGGAAGGG+AGG - chr8_2:49026577-49026596 Msa1252200:CDS 45.0%
GGATTTAGAAGAGGAAGGGA+GGG - chr8_2:49026578-49026597 Msa1252200:CDS 45.0%
GATTTAGAAGAGGAAGGGAG+GGG - chr8_2:49026579-49026598 Msa1252200:CDS 45.0%
TTGAGGGTTTAGGAGGAGAA+TGG + chr8_2:49026770-49026789 None:intergenic 45.0%
GTTTGGATTGAGGGTTTAGG+AGG + chr8_2:49026777-49026796 None:intergenic 45.0%
!!! TGGTGTTTTTTGTGTGTGCG+CGG - chr8_2:49026816-49026835 Msa1252200:intron 45.0%
AGGATCCAAAGCATGGGAAT+TGG - chr8_2:49026895-49026914 Msa1252200:intron 45.0%
ATCCAAAGCATGGGAATTGG+TGG - chr8_2:49026898-49026917 Msa1252200:intron 45.0%
! GGGAATTGGTGGCTAGAATA+TGG - chr8_2:49026909-49026928 Msa1252200:CDS 45.0%
TGGTGGCTAGAATATGGATC+TGG - chr8_2:49026915-49026934 Msa1252200:CDS 45.0%
GCTCACACATCAACCCAAAT+GGG - chr8_2:49027041-49027060 Msa1252200:CDS 45.0%
ACATCAACCCAAATGGGTAG+TGG - chr8_2:49027047-49027066 Msa1252200:CDS 45.0%
TTTACTATGGTGGTCCTGGA+AGG - chr8_2:49027234-49027253 Msa1252200:five_prime_UTR 45.0%
!!! TAAATACATTTTTTATTAAT+GGG + chr8_2:49025001-49025020 None:intergenic 5.0%
!!! TTAAATACATTTTTTATTAA+TGG + chr8_2:49025002-49025021 None:intergenic 5.0%
!!! ATTTTTTTAAACTATTAAAA+AGG - chr8_2:49026521-49026540 Msa1252200:CDS 5.0%
!! ATACATAATTATAATTATAT+AGG - chr8_2:49027286-49027305 Msa1252200:five_prime_UTR 5.0%
GGAGAAGAAACTGAAGGAGG+AGG + chr8_2:49022482-49022501 None:intergenic 50.0%
! GTTTTTCCGCAGAGTCCTGA+TGG - chr8_2:49022730-49022749 Msa1252200:intron 50.0%
CGTGTTAGAATGGACACCAC+CGG - chr8_2:49023107-49023126 Msa1252200:intron 50.0%
TTGTACCTCATGTCCATCGC+CGG + chr8_2:49023129-49023148 None:intergenic 50.0%
AAATGTGTGGGTCCCACAAG+TGG - chr8_2:49024219-49024238 Msa1252200:intron 50.0%
TACCATTGAAGCTGGTTGGC+AGG - chr8_2:49024309-49024328 Msa1252200:intron 50.0%
AGCTATCCCCATAGAGCTCT+GGG + chr8_2:49024873-49024892 None:intergenic 50.0%
TAGCTATCCCCATAGAGCTC+TGG + chr8_2:49024874-49024893 None:intergenic 50.0%
ATGTGGTCAATGCATGGTGG+GGG - chr8_2:49025736-49025755 Msa1252200:intron 50.0%
! CATGGGGCACTCCATTAGTT+TGG - chr8_2:49025759-49025778 Msa1252200:intron 50.0%
ACAAGTGTCCGGGTTCAATG+TGG - chr8_2:49026476-49026495 Msa1252200:CDS 50.0%
GTTAGGGTGTGTTTGGATGG+AGG - chr8_2:49026557-49026576 Msa1252200:CDS 50.0%
AGGGTTTAGGAGGAGAATGG+AGG + chr8_2:49026767-49026786 None:intergenic 50.0%
!!! GGTGTTTTTTGTGTGTGCGC+GGG - chr8_2:49026817-49026836 Msa1252200:intron 50.0%
TGCGTGTAGGATCCAAAGCA+TGG - chr8_2:49026888-49026907 Msa1252200:intron 50.0%
GCGTGTAGGATCCAAAGCAT+GGG - chr8_2:49026889-49026908 Msa1252200:intron 50.0%
AGCCACCAATTCCCATGCTT+TGG + chr8_2:49026903-49026922 None:intergenic 50.0%
GGCTCACACATCAACCCAAA+TGG - chr8_2:49027040-49027059 Msa1252200:CDS 50.0%
GAAGGAGGAGGGAGATGACT+GGG + chr8_2:49022470-49022489 None:intergenic 55.0%
TGAAGGAGGAGGGAGATGAC+TGG + chr8_2:49022471-49022490 None:intergenic 55.0%
TGCCTGCCAACCAGCTTCAA+TGG + chr8_2:49024314-49024333 None:intergenic 55.0%
AGGTCAGCCCAGAGCTCTAT+GGG - chr8_2:49024863-49024882 Msa1252200:intron 55.0%
! TCAATGCATGGTGGGGGCAT+GGG - chr8_2:49025742-49025761 Msa1252200:intron 55.0%
TTAGAAGAGGAAGGGAGGGG+AGG - chr8_2:49026582-49026601 Msa1252200:CDS 55.0%
TAGAAGAGGAAGGGAGGGGA+GGG - chr8_2:49026583-49026602 Msa1252200:CDS 55.0%
TGCTCTCTTGGTCTCTCCGG+AGG + chr8_2:49022939-49022958 None:intergenic 60.0%
AGAATGGACACCACCGGCGA+TGG - chr8_2:49023113-49023132 Msa1252200:intron 60.0%
TACCTCATGTCCATCGCCGG+TGG + chr8_2:49023126-49023145 None:intergenic 60.0%
CAGGTCAGCCCAGAGCTCTA+TGG - chr8_2:49024862-49024881 Msa1252200:intron 60.0%
GGTCAGCCCAGAGCTCTATG+GGG - chr8_2:49024864-49024883 Msa1252200:intron 60.0%
! GTCAATGCATGGTGGGGGCA+TGG - chr8_2:49025741-49025760 Msa1252200:intron 60.0%
! CAATGCATGGTGGGGGCATG+GGG - chr8_2:49025743-49025762 Msa1252200:intron 60.0%
CACCACCGGCGATGGACATG+AGG - chr8_2:49023121-49023140 Msa1252200:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 49022197 49027355 49022197 ID=Msa1252200;Name=Msa1252200
chr8_2 mRNA 49022197 49027355 49022197 ID=Msa1252200-mRNA-1;Parent=Msa1252200;Name=Msa1252200-mRNA-1;_AED=0.07;_eAED=0.07;_QI=145|1|1|1|1|1|7|81|448
chr8_2 exon 49022197 49022655 49022197 ID=Msa1252200-mRNA-1:exon:14836;Parent=Msa1252200-mRNA-1
chr8_2 exon 49024354 49024500 49024354 ID=Msa1252200-mRNA-1:exon:14835;Parent=Msa1252200-mRNA-1
chr8_2 exon 49024634 49024687 49024634 ID=Msa1252200-mRNA-1:exon:14834;Parent=Msa1252200-mRNA-1
chr8_2 exon 49025222 49025386 49025222 ID=Msa1252200-mRNA-1:exon:14833;Parent=Msa1252200-mRNA-1
chr8_2 exon 49026410 49026619 49026410 ID=Msa1252200-mRNA-1:exon:14832;Parent=Msa1252200-mRNA-1
chr8_2 exon 49026724 49026810 49026724 ID=Msa1252200-mRNA-1:exon:14831;Parent=Msa1252200-mRNA-1
chr8_2 exon 49026905 49027355 49026905 ID=Msa1252200-mRNA-1:exon:14830;Parent=Msa1252200-mRNA-1
chr8_2 five_prime_UTR 49027211 49027355 49027211 ID=Msa1252200-mRNA-1:five_prime_utr;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49026905 49027210 49026905 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49026724 49026810 49026724 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49026410 49026619 49026410 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49025222 49025386 49025222 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49024634 49024687 49024634 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49024354 49024500 49024354 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 CDS 49022278 49022655 49022278 ID=Msa1252200-mRNA-1:cds;Parent=Msa1252200-mRNA-1
chr8_2 three_prime_UTR 49022197 49022277 49022197 ID=Msa1252200-mRNA-1:three_prime_utr;Parent=Msa1252200-mRNA-1
Gene Sequence

>Msa1252200

ATGGGGTTAGATTTGTTCCAATGGACAAAACCAAAATATAAGAACACTCAAATTTGTTTTCTTGATGCAAGAAAACAACACAAATGTGGAGAAATACACACAAACACAAACATGGATTTCAGCTTCCCAGTCATCTCCCTCCTCCTTCAGTTTCTTCTCCTTGTTTCTTTGGTTTGTCATGTTTATTCTTCAGAAACAAGTGGCCAAAATCAAACTTTTCTACCAAATGAAGAGTTTCACAAGTTTAGGAGGACCATAAAAATGCATCTTCGACGGATCAACAAGCCTGCTGTTAAGACAATTCAGAGTCCTGATGGTGATATCATAGATTGTGTTATGTCTCATAAACAACCAGCTTTTGATCATCCTATGTTGAAAGGACAAAAACCATTGGATCCTCCGGAGAGACCAAGAGAGCATAAGCAAATGGGCATTGAAAATTATAACTTTCAATTGTGGAGTTTCTCAGGTGAATCATGTCCAGAAGGAACGATTCCGATTAGAAGAATAAAAGAAGATGACATGTTAAGAGCTTGCTCTATTAACACATTTGGAAGAAAATTGAAACGTGTTAGAATGGACACCACCGGCGATGGACATGAGCATGCAGTTGGATATGTGAGTGGGAATGAGTACTATGGAGCAAAAGCTAGCATAAATGTGTGGGTCCCACAAGTGGAAAGTCCATATGAATTCAGCTTGTCTCAAATATGGATCATCTCTGGTTCATTTGGAGTAGATCTCAATACCATTGAAGCTGGTTGGCAGGTCAGCCCAGAGCTCTATGGGGATAGCTACCCTAGATTCTTCACTTATTGGACGACTGATGCATATCAAGGAACTGGGTGCTACAATTTACTTTGCTCTGGCTTTGTTCAAACTAGCAGTAATATTGCAATTGGAGCAGCAATTTCTCCAACTTCTACATATAATGGAGGCCAATTTGATATTAGCTTAATGATCTGGAAGGATCCAAAGCATGGGAATTGGTGGCTAGAATATGGATCTGGAAACTTAATTGGGTATTGGCCATCTTCCTTATTCACACACTTAAAGGATAGTGCAACTATGGTTCAATTTGGTGGAGAAATAGTGAACACAATGTCAACAGGGGCTCACACATCAACCCAAATGGGTAGTGGACATTTTGCTGAAGAGGGTTTTGCAAAAGCTGCATACTTTAGGAATATGCAAGTTGTGGATTCTGATAATAGCTTGATTCCCTTGCCAAATCTCAATGTTGTAGCTAACCGTCCAAATTGTTATAATATACAAGAAGGAACTAATAATGGGTGGGGAAATTACTTTTACTATGGTGGTCCTGGAAGGAATGTAAAATGTCCCTAA
Protein sequence

>Msa1252200

MGLDLFQWTKPKYKNTQICFLDARKQHKCGEIHTNTNMDFSFPVISLLLQFLLLVSLVCHVYSSETSGQNQTFLPNEEFHKFRRTIKMHLRRINKPAVKTIQSPDGDIIDCVMSHKQPAFDHPMLKGQKPLDPPERPREHKQMGIENYNFQLWSFSGESCPEGTIPIRRIKEDDMLRACSINTFGRKLKRVRMDTTGDGHEHAVGYVSGNEYYGAKASINVWVPQVESPYEFSLSQIWIISGSFGVDLNTIEAGWQVSPELYGDSYPRFFTYWTTDAYQGTGCYNLLCSGFVQTSSNIAIGAAISPTSTYNGGQFDISLMIWKDPKHGNWWLEYGSGNLIGYWPSSLFTHLKDSATMVQFGGEIVNTMSTGAHTSTQMGSGHFAEEGFAKAAYFRNMQVVDSDNSLIPLPNLNVVANRPNCYNIQEGTNNGWGNYFYYGGPGRNVKCP*